DNA-directed 5'-3' RNA polymerase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 294 |
| Acc36I | ACCTGC | 1 cut(s) | 217 |
| AciI | CCGC | 3 cut(s) | 14, 67, 770 |
| AclWI | GGATC | 1 cut(s) | 745 |
| AcoI | YGGCCR | 5 cut(s) | 68, 456, 767, 942, 1098 |
| AcsI | RAATTY | 2 cut(s) | 634, 755 |
| AcuI | CTGAAG | 4 cut(s) | 348, 586, 792, 1095 |
| AcyI | GRCGYC | 1 cut(s) | 291 |
| AfaI | GTAC | 3 cut(s) | 74, 143, 1193 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 458, 620, 776 |
| AflII | CTTAAG | 1 cut(s) | 233 |
| AhlI | ACTAGT | 1 cut(s) | 26 |
| AluBI | AGCT | 6 cut(s) | 326, 523, 660, 793, 911, 1213 |
| AluI | AGCT | 6 cut(s) | 326, 523, 660, 793, 911, 1213 |
| Alw21I | GWGCWC | 1 cut(s) | 1323 |
| Alw26I | GTCTC | 3 cut(s) | 298, 915, 1152 |
| AlwI | GGATC | 1 cut(s) | 745 |
| Ama87I | CYCGRG | 2 cut(s) | 741, 1083 |
| AoxI | GGCC | 6 cut(s) | 68, 77, 456, 767, 942, 1098 |
| ApeKI | GCWGC | 3 cut(s) | 239, 323, 1288 |
| ApoI | RAATTY | 2 cut(s) | 634, 755 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 706, 738 |
| AspLEI | GCGC | 1 cut(s) | 1293 |
| AspS9I | GGNCC | 1 cut(s) | 78 |
| AsuHPI | GGTGA | 2 cut(s) | 122, 603 |
| AvaI | CYCGRG | 2 cut(s) | 741, 1083 |
| AxyI | CCTNAGG | 1 cut(s) | 83 |
| BalI | TGGCCA | 2 cut(s) | 944, 1100 |
| BbsI | GAAGAC | 2 cut(s) | 721, 858 |
| Bbv12I | GWGCWC | 1 cut(s) | 1323 |
| BbvI | GCAGC | 3 cut(s) | 226, 335, 1275 |
| BccI | CCATC | 3 cut(s) | 47, 355, 1109 |
| BceAI | ACGGC | 1 cut(s) | 55 |
| BcoDI | GTCTC | 3 cut(s) | 298, 915, 1152 |
| BcuI | ACTAGT | 1 cut(s) | 26 |
| BfaI | CTAG | 3 cut(s) | 27, 425, 858 |
| BfrI | CTTAAG | 1 cut(s) | 233 |
| BfuAI | ACCTGC | 1 cut(s) | 217 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 76, 1106 |
| BglII | AGATCT | 1 cut(s) | 264 |
| BisI | GCNGC | 6 cut(s) | 14, 68, 240, 324, 770, 1289 |
| BlpI | GCTNAGC | 2 cut(s) | 686, 1214 |
| BlsI | GCNGC | 6 cut(s) | 15, 69, 241, 325, 771, 1290 |
| BmeT110I | CYCGRG | 2 cut(s) | 741, 1083 |
| BmgT120I | GGNCC | 1 cut(s) | 78 |
| BmiI | GGNNCC | 3 cut(s) | 80, 161, 1068 |
| BmsI | GCATC | 5 cut(s) | 355, 484, 616, 878, 987 |
| BpiI | GAAGAC | 2 cut(s) | 721, 858 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 686, 1214 |
| BpuEI | CTTGAG | 3 cut(s) | 540, 923, 1309 |
| BsaHI | GRCGYC | 1 cut(s) | 291 |
| BsaI | GGTCTC | 1 cut(s) | 1152 |
| BsaJI | CCNNGG | 2 cut(s) | 285, 1162 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 458, 620, 776 |
| Bse118I | RCCGGY | 1 cut(s) | 75 |
| Bse1I | ACTGG | 3 cut(s) | 274, 495, 968 |
| Bse21I | CCTNAGG | 1 cut(s) | 83 |
| BseDI | CCNNGG | 2 cut(s) | 285, 1162 |
| BseGI | GGATG | 2 cut(s) | 370, 499 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 458, 620, 776 |
| BseMII | CTCAG | 4 cut(s) | 74, 272, 1027, 1105 |
| BseNI | ACTGG | 3 cut(s) | 274, 495, 968 |
| BseRI | GAGGAG | 7 cut(s) | 163, 166, 169, 172, 803, 1059, 1168 |
| BseX3I | CGGCCG | 1 cut(s) | 68 |
| BseXI | GCAGC | 3 cut(s) | 226, 335, 1275 |
| Bsh1285I | CGRYCG | 2 cut(s) | 64, 71 |
| BshFI | GGCC | 6 cut(s) | 70, 79, 458, 769, 944, 1100 |
| BsiEI | CGRYCG | 2 cut(s) | 64, 71 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1323 |
| BsiHKCI | CYCGRG | 2 cut(s) | 741, 1083 |
| BsiSI | CCGG | 2 cut(s) | 76, 459 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 458, 620, 776 |
| BsmAI | GTCTC | 3 cut(s) | 298, 915, 1152 |
| BsmBI | CGTCTC | 1 cut(s) | 298 |
| BsmI | GAATGC | 1 cut(s) | 453 |
| BsnI | GGCC | 6 cut(s) | 70, 79, 458, 769, 944, 1100 |
| Bso31I | GGTCTC | 1 cut(s) | 1152 |
| BsoBI | CYCGRG | 2 cut(s) | 741, 1083 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1323 |
| Bsp143I | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 686, 1214 |
| Bsp19I | CCATGG | 1 cut(s) | 1162 |
| BspACI | CCGC | 3 cut(s) | 14, 67, 770 |
| BspANI | GGCC | 6 cut(s) | 70, 79, 458, 769, 944, 1100 |
| BspCNI | CTCAG | 4 cut(s) | 75, 271, 1026, 1104 |
| BspHI | TCATGA | 1 cut(s) | 1033 |
| BspLI | GGNNCC | 3 cut(s) | 80, 161, 1068 |
| BspMI | ACCTGC | 1 cut(s) | 217 |
| BspPI | GGATC | 1 cut(s) | 745 |
| BspTI | CTTAAG | 1 cut(s) | 233 |
| BspTNI | GGTCTC | 1 cut(s) | 1152 |
| BsrFI | RCCGGY | 1 cut(s) | 75 |
| BsrI | ACTGG | 3 cut(s) | 274, 495, 968 |
| BssAI | RCCGGY | 1 cut(s) | 75 |
| BssECI | CCNNGG | 2 cut(s) | 285, 1162 |
| BssMI | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| BssNI | GRCGYC | 1 cut(s) | 291 |
| BssT1I | CCWWGG | 2 cut(s) | 285, 1162 |
| Bst4CI | ACNGT | 4 cut(s) | 56, 439, 818, 894 |
| Bst6I | CTCTTC | 1 cut(s) | 1239 |
| BstACI | GRCGYC | 1 cut(s) | 291 |
| BstAFI | CTTAAG | 1 cut(s) | 233 |
| BstDEI | CTNAG | 9 cut(s) | 83, 258, 649, 656, 686, 719, 1013, 1091, 1214 |
| BstDSI | CCRYGG | 1 cut(s) | 1162 |
| BstEII | GGTNACC | 1 cut(s) | 609 |
| BstF5I | GGATG | 2 cut(s) | 370, 499 |
| BstHHI | GCGC | 1 cut(s) | 1293 |
| BstKTI | GATC | 7 cut(s) | 64, 94, 267, 740, 765, 863, 983 |
| BstMAI | GTCTC | 3 cut(s) | 298, 915, 1152 |
| BstMBI | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| BstMCI | CGRYCG | 2 cut(s) | 64, 71 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 76, 185, 344, 1106 |
| BstNSI | RCATGY | 1 cut(s) | 974 |
| BstPI | GGTNACC | 1 cut(s) | 609 |
| BstV1I | GCAGC | 3 cut(s) | 226, 335, 1275 |
| BstV2I | GAAGAC | 2 cut(s) | 721, 858 |
| BstX2I | RGATCY | 2 cut(s) | 264, 737 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1143 |
| BstYI | RGATCY | 2 cut(s) | 264, 737 |
| BstZI | CGGCCG | 1 cut(s) | 68 |
| Bsu36I | CCTNAGG | 1 cut(s) | 83 |
| BsuRI | GGCC | 6 cut(s) | 70, 79, 458, 769, 944, 1100 |
| BtgI | CCRYGG | 1 cut(s) | 1162 |
| BtsCI | GGATG | 2 cut(s) | 370, 499 |
| BtsI | GCAGTG | 1 cut(s) | 576 |
| BtsIMutI | CAGTG | 5 cut(s) | 17, 576, 890, 961, 1053 |
| BveI | ACCTGC | 1 cut(s) | 217 |
| CciI | TCATGA | 1 cut(s) | 1033 |
| CfoI | GCGC | 1 cut(s) | 1293 |
| Cfr10I | RCCGGY | 1 cut(s) | 75 |
| Cfr13I | GGNCC | 1 cut(s) | 78 |
| Csp6I | GTAC | 3 cut(s) | 73, 142, 1192 |
| CviAII | CATG | 9 cut(s) | 473, 971, 989, 1034, 1105, 1163, 1168, 1225, 1305 |
| CviQI | GTAC | 3 cut(s) | 73, 142, 1192 |
| DdeI | CTNAG | 9 cut(s) | 83, 258, 649, 656, 686, 719, 1013, 1091, 1214 |
| DpnI | GATC | 7 cut(s) | 63, 93, 266, 739, 764, 862, 982 |
| DpnII | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| EaeI | YGGCCR | 5 cut(s) | 68, 456, 767, 942, 1098 |
| EagI | CGGCCG | 1 cut(s) | 68 |
| Eam1104I | CTCTTC | 1 cut(s) | 1239 |
| EarI | CTCTTC | 1 cut(s) | 1239 |
| EclXI | CGGCCG | 1 cut(s) | 68 |
| Eco130I | CCWWGG | 2 cut(s) | 285, 1162 |
| Eco31I | GGTCTC | 1 cut(s) | 1152 |
| Eco32I | GATATC | 1 cut(s) | 782 |
| Eco52I | CGGCCG | 1 cut(s) | 68 |
| Eco57I | CTGAAG | 4 cut(s) | 348, 586, 792, 1095 |
| Eco81I | CCTNAGG | 1 cut(s) | 83 |
| Eco88I | CYCGRG | 2 cut(s) | 741, 1083 |
| Eco91I | GGTNACC | 1 cut(s) | 609 |
| EcoO65I | GGTNACC | 1 cut(s) | 609 |
| EcoRI | GAATTC | 1 cut(s) | 634 |
| EcoRV | GATATC | 1 cut(s) | 782 |
| EcoT14I | CCWWGG | 2 cut(s) | 285, 1162 |
| EcoT22I | ATGCAT | 1 cut(s) | 1226 |
| ErhI | CCWWGG | 2 cut(s) | 285, 1162 |
| Esp3I | CGTCTC | 1 cut(s) | 298 |
| FaeI | CATG | 9 cut(s) | 476, 974, 992, 1037, 1108, 1166, 1171, 1228, 1308 |
| FatI | CATG | 9 cut(s) | 472, 970, 988, 1033, 1104, 1162, 1167, 1224, 1304 |
| FauNDI | CATATG | 1 cut(s) | 1220 |
| Fnu4HI | GCNGC | 6 cut(s) | 14, 68, 240, 324, 770, 1289 |
| FokI | GGATG | 2 cut(s) | 377, 506 |
| Fsp4HI | GCNGC | 6 cut(s) | 14, 68, 240, 324, 770, 1289 |
| FspBI | CTAG | 3 cut(s) | 27, 425, 858 |
| GlaI | GCGC | 1 cut(s) | 1292 |
| GluI | GCNGC | 6 cut(s) | 14, 68, 240, 324, 770, 1289 |
| HaeIII | GGCC | 6 cut(s) | 70, 79, 458, 769, 944, 1100 |
| HapII | CCGG | 2 cut(s) | 76, 459 |
| HhaI | GCGC | 1 cut(s) | 1293 |
| Hin1I | GRCGYC | 1 cut(s) | 291 |
| Hin1II | CATG | 9 cut(s) | 476, 974, 992, 1037, 1108, 1166, 1171, 1228, 1308 |
| Hin6I | GCGC | 1 cut(s) | 1291 |
| HinP1I | GCGC | 1 cut(s) | 1291 |
| HincII | GTYRAC | 1 cut(s) | 1331 |
| HindII | GTYRAC | 1 cut(s) | 1331 |
| HinfI | GANTC | 4 cut(s) | 590, 1058, 1126, 1298 |
| HpaII | CCGG | 2 cut(s) | 76, 459 |
| HphI | GGTGA | 2 cut(s) | 122, 603 |
| Hpy166II | GTNNAC | 3 cut(s) | 255, 1024, 1331 |
| Hpy188I | TCNGA | 4 cut(s) | 605, 726, 874, 1114 |
| Hpy8I | GTNNAC | 3 cut(s) | 255, 1024, 1331 |
| HpyAV | CCTTC | 4 cut(s) | 280, 600, 834, 1192 |
| HpyCH4III | ACNGT | 4 cut(s) | 56, 439, 818, 894 |
| HpyCH4IV | ACGT | 2 cut(s) | 291, 1020 |
| HpyCH4V | TGCA | 7 cut(s) | 302, 629, 646, 677, 827, 1132, 1224 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 76, 185, 344, 1106 |
| HpyF3I | CTNAG | 9 cut(s) | 83, 258, 649, 656, 686, 719, 1013, 1091, 1214 |
| HpySE526I | ACGT | 2 cut(s) | 291, 1020 |
| Hsp92I | GRCGYC | 1 cut(s) | 291 |
| Hsp92II | CATG | 9 cut(s) | 476, 974, 992, 1037, 1108, 1166, 1171, 1228, 1308 |
| HspAI | GCGC | 1 cut(s) | 1291 |
| Kzo9I | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| LmnI | GCTCC | 4 cut(s) | 50, 159, 790, 1072 |
| Lsp1109I | GCAGC | 3 cut(s) | 226, 335, 1275 |
| LweI | GCATC | 5 cut(s) | 355, 484, 616, 878, 987 |
| MaeI | CTAG | 3 cut(s) | 27, 425, 858 |
| MaeII | ACGT | 2 cut(s) | 291, 1020 |
| MaeIII | GTNAC | 4 cut(s) | 56, 609, 802, 1074 |
| MalI | GATC | 7 cut(s) | 63, 93, 266, 739, 764, 862, 982 |
| MboI | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| MboII | GAAGA | 8 cut(s) | 127, 174, 341, 726, 764, 863, 1253, 1256 |
| MfeI | CAATTG | 1 cut(s) | 828 |
| MflI | RGATCY | 2 cut(s) | 264, 737 |
| MhlI | GDGCHC | 1 cut(s) | 1323 |
| MlsI | TGGCCA | 2 cut(s) | 944, 1100 |
| MluNI | TGGCCA | 2 cut(s) | 944, 1100 |
| MlyI | GAGTC | 1 cut(s) | 599 |
| MmeI | TCCRAC | 1 cut(s) | 77 |
| Mox20I | TGGCCA | 2 cut(s) | 944, 1100 |
| Mph1103I | ATGCAT | 1 cut(s) | 1226 |
| MscI | TGGCCA | 2 cut(s) | 944, 1100 |
| MseI | TTAA | 4 cut(s) | 234, 351, 878, 1345 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1223 |
| Msp20I | TGGCCA | 2 cut(s) | 944, 1100 |
| MspA1I | CMGCKG | 1 cut(s) | 326 |
| MspCI | CTTAAG | 1 cut(s) | 233 |
| MspI | CCGG | 2 cut(s) | 76, 459 |
| MunI | CAATTG | 1 cut(s) | 828 |
| Mva1269I | GAATGC | 1 cut(s) | 453 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 76, 185, 344, 1106 |
| NcoI | CCATGG | 1 cut(s) | 1162 |
| NdeI | CATATG | 1 cut(s) | 1220 |
| NdeII | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| NlaIII | CATG | 9 cut(s) | 476, 974, 992, 1037, 1108, 1166, 1171, 1228, 1308 |
| NlaIV | GGNNCC | 3 cut(s) | 80, 161, 1068 |
| NmuCI | GTSAC | 2 cut(s) | 56, 609 |
| NsiI | ATGCAT | 1 cut(s) | 1226 |
| NspI | RCATGY | 1 cut(s) | 974 |
| PaeR7I | CTCGAG | 1 cut(s) | 741 |
| PagI | TCATGA | 1 cut(s) | 1033 |
| PctI | GAATGC | 1 cut(s) | 453 |
| PfeI | GAWTC | 3 cut(s) | 1058, 1126, 1298 |
| PflFI | GACNNNGTC | 2 cut(s) | 720, 816 |
| PkrI | GCNGC | 6 cut(s) | 15, 69, 241, 325, 771, 1290 |
| Ple19I | CGATCG | 1 cut(s) | 64 |
| PleI | GAGTC | 1 cut(s) | 598 |
| PpsI | GAGTC | 1 cut(s) | 598 |
| PspEI | GGTNACC | 1 cut(s) | 609 |
| PspN4I | GGNNCC | 3 cut(s) | 80, 161, 1068 |
| PspPI | GGNCC | 1 cut(s) | 78 |
| PsuI | RGATCY | 2 cut(s) | 264, 737 |
| PsyI | GACNNNGTC | 2 cut(s) | 720, 816 |
| PvuI | CGATCG | 1 cut(s) | 64 |
| PvuII | CAGCTG | 1 cut(s) | 326 |
| RsaI | GTAC | 3 cut(s) | 74, 143, 1193 |
| RsaNI | GTAC | 3 cut(s) | 73, 142, 1192 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SaqAI | TTAA | 4 cut(s) | 234, 351, 878, 1345 |
| SatI | GCNGC | 6 cut(s) | 14, 68, 240, 324, 770, 1289 |
| Sau3AI | GATC | 7 cut(s) | 61, 91, 264, 737, 762, 860, 980 |
| Sau96I | GGNCC | 1 cut(s) | 78 |
| SchI | GAGTC | 1 cut(s) | 599 |
| SduI | GDGCHC | 1 cut(s) | 1323 |
| SfaNI | GCATC | 5 cut(s) | 355, 484, 616, 878, 987 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 76 |
| Sfr274I | CTCGAG | 1 cut(s) | 741 |
| SlaI | CTCGAG | 1 cut(s) | 741 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SmlI | CTYRAG | 5 cut(s) | 233, 555, 741, 902, 1324 |
| SmoI | CTYRAG | 5 cut(s) | 233, 555, 741, 902, 1324 |
| SpeI | ACTAGT | 1 cut(s) | 26 |
| SsiI | CCGC | 3 cut(s) | 14, 67, 770 |
| SspMI | CTAG | 3 cut(s) | 27, 425, 858 |
| StyI | CCWWGG | 2 cut(s) | 285, 1162 |
| TaaI | ACNGT | 4 cut(s) | 56, 439, 818, 894 |
| TaiI | ACGT | 2 cut(s) | 294, 1023 |
| TaqI | TCGA | 5 cut(s) | 128, 355, 742, 849, 1151 |
| TauI | GCSGC | 3 cut(s) | 16, 70, 772 |
| TfiI | GAWTC | 3 cut(s) | 1058, 1126, 1298 |
| Tru1I | TTAA | 4 cut(s) | 234, 351, 878, 1345 |
| Tru9I | TTAA | 4 cut(s) | 234, 351, 878, 1345 |
| TscAI | CASTG | 5 cut(s) | 24, 576, 897, 968, 1060 |
| TseFI | GTSAC | 2 cut(s) | 56, 609 |
| TseI | GCWGC | 3 cut(s) | 239, 323, 1288 |
| Tsp45I | GTSAC | 2 cut(s) | 56, 609 |
| TspDTI | ATGAA | 3 cut(s) | 368, 399, 627 |
| TspRI | CASTG | 5 cut(s) | 24, 576, 897, 968, 1060 |
| Tth111I | GACNNNGTC | 2 cut(s) | 720, 816 |
| Vha464I | CTTAAG | 1 cut(s) | 233 |
| XapI | RAATTY | 2 cut(s) | 634, 755 |
| XceI | RCATGY | 1 cut(s) | 974 |
| XhoI | CTCGAG | 1 cut(s) | 741 |
| XspI | CTAG | 3 cut(s) | 27, 425, 858 |
| ZraI | GACGTC | 1 cut(s) | 292 |
| Zsp2I | ATGCAT | 1 cut(s) | 1226 |