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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 14, 95 |
| AciI | CCGC | 2 cut(s) | 148, 194 |
| AclWI | GGATC | 5 cut(s) | 227, 707, 1169, 1475, 1784 |
| AcoI | YGGCCR | 2 cut(s) | 1322, 1937 |
| AcsI | RAATTY | 1 cut(s) | 1405 |
| AcuI | CTGAAG | 3 cut(s) | 660, 1479, 1578 |
| AflIII | ACRYGT | 1 cut(s) | 1550 |
| AgeI | ACCGGT | 1 cut(s) | 56 |
| AgsI | TTSAA | 8 cut(s) | 230, 247, 308, 314, 779, 1429, 1601, 1785 |
| AjnI | CCWGG | 2 cut(s) | 853, 1792 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1593, 1625, 1902, 1934 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1697, 1729 |
| Alw21I | GWGCWC | 1 cut(s) | 933 |
| Alw26I | GTCTC | 4 cut(s) | 113, 1010, 1062, 1567 |
| AlwI | GGATC | 5 cut(s) | 227, 707, 1169, 1475, 1784 |
| Ama87I | CYCGRG | 1 cut(s) | 1077 |
| AoxI | GGCC | 6 cut(s) | 373, 1268, 1322, 1628, 1883, 1937 |
| ApaI | GGGCCC | 1 cut(s) | 377 |
| ApeKI | GCWGC | 2 cut(s) | 458, 641 |
| ApoI | RAATTY | 1 cut(s) | 1405 |
| AseI | ATTAAT | 1 cut(s) | 1001 |
| AsiGI | ACCGGT | 1 cut(s) | 56 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 431, 480, 1257 |
| AspS9I | GGNCC | 9 cut(s) | 158, 196, 260, 289, 373, 374, 1208, 1514, 1823 |
| AsuHPI | GGTGA | 3 cut(s) | 341, 761, 780 |
| AvaI | CYCGRG | 1 cut(s) | 1077 |
| AvaII | GGWCC | 7 cut(s) | 158, 196, 260, 289, 1208, 1514, 1823 |
| BaeGI | GKGCMC | 1 cut(s) | 377 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 717, 750 |
| BalI | TGGCCA | 2 cut(s) | 1324, 1939 |
| BanII | GRGCYC | 1 cut(s) | 377 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 127, 159, 868, 900 |
| BbsI | GAAGAC | 1 cut(s) | 1773 |
| Bbv12I | GWGCWC | 1 cut(s) | 933 |
| BbvI | GCAGC | 2 cut(s) | 470, 653 |
| BccI | CCATC | 4 cut(s) | 149, 679, 716, 1948 |
| BciT130I | CCWGG | 2 cut(s) | 855, 1794 |
| BcoDI | GTCTC | 4 cut(s) | 113, 1010, 1062, 1567 |
| BfaI | CTAG | 3 cut(s) | 915, 938, 1103 |
| BfuAI | ACCTGC | 2 cut(s) | 14, 95 |
| BisI | GCNGC | 2 cut(s) | 459, 642 |
| BlsI | GCNGC | 2 cut(s) | 460, 643 |
| Bme1390I | CCNGG | 2 cut(s) | 855, 1794 |
| Bme18I | GGWCC | 7 cut(s) | 158, 196, 260, 289, 1208, 1514, 1823 |
| BmeT110I | CYCGRG | 1 cut(s) | 1077 |
| BmgT120I | GGNCC | 9 cut(s) | 158, 196, 260, 289, 373, 374, 1208, 1514, 1823 |
| BmiI | GGNNCC | 3 cut(s) | 291, 375, 1863 |
| BmrFI | CCNGG | 2 cut(s) | 855, 1794 |
| BmsI | GCATC | 6 cut(s) | 70, 181, 218, 811, 1479, 1903 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1777 |
| BpiI | GAAGAC | 1 cut(s) | 1773 |
| BpmI | CTGGAG | 1 cut(s) | 917 |
| BpuEI | CTTGAG | 2 cut(s) | 1044, 1287 |
| BsaBI | GATNNNNATC | 1 cut(s) | 93 |
| BsaI | GGTCTC | 3 cut(s) | 113, 1062, 1567 |
| BsaJI | CCNNGG | 1 cut(s) | 1856 |
| BsaWI | WCCGGW | 1 cut(s) | 56 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1697, 1727 |
| Bse118I | RCCGGY | 1 cut(s) | 56 |
| Bse1I | ACTGG | 1 cut(s) | 1170 |
| Bse3DI | GCAATG | 1 cut(s) | 1224 |
| Bse8I | GATNNNNATC | 1 cut(s) | 93 |
| BseBI | CCWGG | 2 cut(s) | 855, 1794 |
| BseDI | CCNNGG | 1 cut(s) | 1856 |
| BseGI | GGATG | 3 cut(s) | 94, 794, 1144 |
| BseJI | GATNNNNATC | 1 cut(s) | 93 |
| BseMI | GCAATG | 1 cut(s) | 1224 |
| BseMII | CTCAG | 3 cut(s) | 285, 1353, 1884 |
| BseNI | ACTGG | 1 cut(s) | 1170 |
| BseRI | GAGGAG | 2 cut(s) | 894, 1191 |
| BseSI | GKGCMC | 1 cut(s) | 377 |
| BseXI | GCAGC | 2 cut(s) | 470, 653 |
| BshFI | GGCC | 6 cut(s) | 375, 1270, 1324, 1630, 1885, 1939 |
| BshTI | ACCGGT | 1 cut(s) | 56 |
| BsiHKAI | GWGCWC | 1 cut(s) | 933 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1077 |
| BsiSI | CCGG | 1 cut(s) | 57 |
| BslFI | GGGAC | 1 cut(s) | 932 |
| BsmAI | GTCTC | 4 cut(s) | 113, 1010, 1062, 1567 |
| BsmFI | GGGAC | 1 cut(s) | 932 |
| BsnI | GGCC | 6 cut(s) | 375, 1270, 1324, 1630, 1885, 1939 |
| Bso31I | GGTCTC | 3 cut(s) | 113, 1062, 1567 |
| BsoBI | CYCGRG | 1 cut(s) | 1077 |
| Bsp120I | GGGCCC | 1 cut(s) | 373 |
| Bsp1286I | GDGCHC | 2 cut(s) | 377, 933 |
| Bsp1407I | TGTACA | 1 cut(s) | 214 |
| Bsp143I | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| BspACI | CCGC | 2 cut(s) | 148, 194 |
| BspANI | GGCC | 6 cut(s) | 375, 1270, 1324, 1630, 1885, 1939 |
| BspCNI | CTCAG | 3 cut(s) | 286, 1354, 1885 |
| BspHI | TCATGA | 1 cut(s) | 1012 |
| BspLI | GGNNCC | 3 cut(s) | 291, 375, 1863 |
| BspMI | ACCTGC | 2 cut(s) | 14, 95 |
| BspPI | GGATC | 5 cut(s) | 227, 707, 1169, 1475, 1784 |
| BspQI | GCTCTTC | 1 cut(s) | 936 |
| BspTNI | GGTCTC | 3 cut(s) | 113, 1062, 1567 |
| BsrDI | GCAATG | 1 cut(s) | 1224 |
| BsrFI | RCCGGY | 1 cut(s) | 56 |
| BsrGI | TGTACA | 1 cut(s) | 214 |
| BsrI | ACTGG | 1 cut(s) | 1170 |
| BssAI | RCCGGY | 1 cut(s) | 56 |
| BssECI | CCNNGG | 1 cut(s) | 1856 |
| BssMI | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| BssT1I | CCWWGG | 1 cut(s) | 1856 |
| Bst2UI | CCWGG | 2 cut(s) | 855, 1794 |
| Bst4CI | ACNGT | 2 cut(s) | 226, 912 |
| Bst6I | CTCTTC | 2 cut(s) | 871, 936 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1631 |
| BstAUI | TGTACA | 1 cut(s) | 214 |
| BstC8I | GCNNGC | 4 cut(s) | 1115, 1326, 1632, 1799 |
| BstDEI | CTNAG | 5 cut(s) | 48, 294, 988, 1362, 1893 |
| BstF5I | GGATG | 3 cut(s) | 94, 794, 1144 |
| BstKTI | GATC | 5 cut(s) | 235, 702, 1177, 1483, 1792 |
| BstMAI | GTCTC | 4 cut(s) | 113, 1010, 1062, 1567 |
| BstMBI | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 943, 1325, 1631 |
| BstNI | CCWGG | 2 cut(s) | 855, 1794 |
| BstNSI | RCATGY | 4 cut(s) | 395, 615, 929, 1554 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1777 |
| BstSCI | CCNGG | 2 cut(s) | 853, 1792 |
| BstSLI | GKGCMC | 1 cut(s) | 377 |
| BstV1I | GCAGC | 2 cut(s) | 470, 653 |
| BstV2I | GAAGAC | 1 cut(s) | 1773 |
| BstX2I | RGATCY | 1 cut(s) | 232 |
| BstYI | RGATCY | 1 cut(s) | 232 |
| BsuRI | GGCC | 6 cut(s) | 375, 1270, 1324, 1630, 1885, 1939 |
| BtsCI | GGATG | 3 cut(s) | 94, 794, 1144 |
| BtsIMutI | CAGTG | 3 cut(s) | 684, 951, 1177 |
| BveI | ACCTGC | 2 cut(s) | 14, 95 |
| Cac8I | GCNNGC | 4 cut(s) | 1115, 1326, 1632, 1799 |
| CciI | TCATGA | 1 cut(s) | 1012 |
| Cfr10I | RCCGGY | 1 cut(s) | 56 |
| Cfr13I | GGNCC | 9 cut(s) | 158, 196, 260, 289, 373, 374, 1208, 1514, 1823 |
| CspAI | ACCGGT | 1 cut(s) | 56 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1417, 1452 |
| DdeI | CTNAG | 5 cut(s) | 48, 294, 988, 1362, 1893 |
| DpnI | GATC | 5 cut(s) | 234, 701, 1176, 1482, 1791 |
| DpnII | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| EaeI | YGGCCR | 2 cut(s) | 1322, 1937 |
| Eam1104I | CTCTTC | 2 cut(s) | 871, 936 |
| EarI | CTCTTC | 2 cut(s) | 871, 936 |
| Eco130I | CCWWGG | 1 cut(s) | 1856 |
| Eco147I | AGGCCT | 1 cut(s) | 1270 |
| Eco24I | GRGCYC | 1 cut(s) | 377 |
| Eco31I | GGTCTC | 3 cut(s) | 113, 1062, 1567 |
| Eco32I | GATATC | 2 cut(s) | 1543, 1852 |
| Eco47I | GGWCC | 7 cut(s) | 158, 196, 260, 289, 1208, 1514, 1823 |
| Eco57I | CTGAAG | 3 cut(s) | 660, 1479, 1578 |
| Eco88I | CYCGRG | 1 cut(s) | 1077 |
| EcoO109I | RGGNCCY | 2 cut(s) | 374, 1823 |
| EcoRI | GAATTC | 1 cut(s) | 1405 |
| EcoRII | CCWGG | 2 cut(s) | 853, 1792 |
| EcoRV | GATATC | 2 cut(s) | 1543, 1852 |
| EcoT14I | CCWWGG | 1 cut(s) | 1856 |
| EcoT22I | ATGCAT | 1 cut(s) | 211 |
| EcoT38I | GRGCYC | 1 cut(s) | 377 |
| ErhI | CCWWGG | 1 cut(s) | 1856 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 465, 497, 810, 842, 1245, 1277, 1715, 1747 |
| FaqI | GGGAC | 1 cut(s) | 932 |
| FauI | CCCGC | 1 cut(s) | 155 |
| FauNDI | CATATG | 1 cut(s) | 337 |
| Fnu4HI | GCNGC | 2 cut(s) | 459, 642 |
| FokI | GGATG | 3 cut(s) | 101, 781, 1151 |
| FriOI | GRGCYC | 1 cut(s) | 377 |
| Fsp4HI | GCNGC | 2 cut(s) | 459, 642 |
| FspBI | CTAG | 3 cut(s) | 915, 938, 1103 |
| GluI | GCNGC | 2 cut(s) | 459, 642 |
| GsuI | CTGGAG | 1 cut(s) | 917 |
| HaeIII | GGCC | 6 cut(s) | 375, 1270, 1324, 1630, 1885, 1939 |
| HapII | CCGG | 1 cut(s) | 57 |
| HincII | GTYRAC | 1 cut(s) | 1066 |
| HindII | GTYRAC | 1 cut(s) | 1066 |
| HindIII | AAGCTT | 3 cut(s) | 141, 355, 1254 |
| HinfI | GANTC | 4 cut(s) | 94, 298, 304, 969 |
| HpaII | CCGG | 1 cut(s) | 57 |
| HphI | GGTGA | 3 cut(s) | 341, 761, 780 |
| Hpy166II | GTNNAC | 5 cut(s) | 332, 596, 728, 1066, 1705 |
| Hpy188I | TCNGA | 7 cut(s) | 184, 303, 437, 1179, 1363, 1514, 1894 |
| Hpy188III | TCNNGA | 7 cut(s) | 274, 535, 1013, 1077, 1103, 1556, 1752 |
| Hpy8I | GTNNAC | 5 cut(s) | 332, 596, 728, 1066, 1705 |
| HpyAV | CCTTC | 6 cut(s) | 913, 1148, 1398, 1454, 1704, 1763 |
| HpyCH4III | ACNGT | 2 cut(s) | 226, 912 |
| HpyCH4IV | ACGT | 1 cut(s) | 1159 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 943, 1325, 1631 |
| HpyF3I | CTNAG | 5 cut(s) | 48, 294, 988, 1362, 1893 |
| HpySE526I | ACGT | 1 cut(s) | 1159 |
| Kzo9I | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| LguI | GCTCTTC | 1 cut(s) | 936 |
| LmnI | GCTCC | 1 cut(s) | 936 |
| Lsp1109I | GCAGC | 2 cut(s) | 470, 653 |
| LweI | GCATC | 6 cut(s) | 70, 181, 218, 811, 1479, 1903 |
| MaeI | CTAG | 3 cut(s) | 915, 938, 1103 |
| MaeII | ACGT | 1 cut(s) | 1159 |
| MaeIII | GTNAC | 2 cut(s) | 569, 883 |
| MalI | GATC | 5 cut(s) | 234, 701, 1176, 1482, 1791 |
| MboI | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| MfeI | CAATTG | 1 cut(s) | 1429 |
| MflI | RGATCY | 1 cut(s) | 232 |
| MhlI | GDGCHC | 2 cut(s) | 377, 933 |
| MlsI | TGGCCA | 2 cut(s) | 1324, 1939 |
| MluNI | TGGCCA | 2 cut(s) | 1324, 1939 |
| MlyI | GAGTC | 1 cut(s) | 307 |
| MmeI | TCCRAC | 4 cut(s) | 281, 287, 575, 1657 |
| Mox20I | TGGCCA | 2 cut(s) | 1324, 1939 |
| Mph1103I | ATGCAT | 1 cut(s) | 211 |
| MroXI | GAANNNNTTC | 3 cut(s) | 431, 480, 1257 |
| MscI | TGGCCA | 2 cut(s) | 1324, 1939 |
| MseI | TTAA | 8 cut(s) | 318, 410, 558, 690, 767, 863, 1001, 1497 |
| MslI | CAYNNNNRTG | 4 cut(s) | 610, 956, 1567, 1905 |
| Msp20I | TGGCCA | 2 cut(s) | 1324, 1939 |
| MspA1I | CMGCKG | 2 cut(s) | 644, 1328 |
| MspI | CCGG | 1 cut(s) | 57 |
| MspR9I | CCNGG | 2 cut(s) | 855, 1794 |
| MunI | CAATTG | 1 cut(s) | 1429 |
| MvaI | CCWGG | 2 cut(s) | 855, 1794 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 943, 1325, 1631 |
| NdeI | CATATG | 1 cut(s) | 337 |
| NdeII | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| NlaIV | GGNNCC | 3 cut(s) | 291, 375, 1863 |
| NmuCI | GTSAC | 1 cut(s) | 569 |
| NsiI | ATGCAT | 1 cut(s) | 211 |
| NspI | RCATGY | 4 cut(s) | 395, 615, 929, 1554 |
| PaeR7I | CTCGAG | 1 cut(s) | 1077 |
| PagI | TCATGA | 1 cut(s) | 1012 |
| PceI | AGGCCT | 1 cut(s) | 1270 |
| PciI | ACATGT | 1 cut(s) | 1550 |
| PciSI | GCTCTTC | 1 cut(s) | 936 |
| PdmI | GAANNNNTTC | 3 cut(s) | 431, 480, 1257 |
| PfeI | GAWTC | 3 cut(s) | 94, 304, 969 |
| PinAI | ACCGGT | 1 cut(s) | 56 |
| PkrI | GCNGC | 2 cut(s) | 460, 643 |
| PleI | GAGTC | 1 cut(s) | 306 |
| PpsI | GAGTC | 1 cut(s) | 306 |
| PpuMI | RGGWCCY | 1 cut(s) | 1823 |
| PscI | ACATGT | 1 cut(s) | 1550 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1777 |
| PshBI | ATTAAT | 1 cut(s) | 1001 |
| Psp5II | RGGWCCY | 1 cut(s) | 1823 |
| Psp6I | CCWGG | 2 cut(s) | 853, 1792 |
| PspGI | CCWGG | 2 cut(s) | 853, 1792 |
| PspN4I | GGNNCC | 3 cut(s) | 291, 375, 1863 |
| PspOMI | GGGCCC | 1 cut(s) | 373 |
| PspPI | GGNCC | 9 cut(s) | 158, 196, 260, 289, 373, 374, 1208, 1514, 1823 |
| PspPPI | RGGWCCY | 1 cut(s) | 1823 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 163, 195 |
| PsuI | RGATCY | 1 cut(s) | 232 |
| PvuII | CAGCTG | 2 cut(s) | 644, 1328 |
| RseI | CAYNNNNRTG | 4 cut(s) | 610, 956, 1567, 1905 |
| SapI | GCTCTTC | 1 cut(s) | 936 |
| SaqAI | TTAA | 8 cut(s) | 318, 410, 558, 690, 767, 863, 1001, 1497 |
| SatI | GCNGC | 2 cut(s) | 459, 642 |
| Sau3AI | GATC | 5 cut(s) | 232, 699, 1174, 1480, 1789 |
| Sau96I | GGNCC | 9 cut(s) | 158, 196, 260, 289, 373, 374, 1208, 1514, 1823 |
| SchI | GAGTC | 1 cut(s) | 307 |
| ScrFI | CCNGG | 2 cut(s) | 855, 1794 |
| SduI | GDGCHC | 2 cut(s) | 377, 933 |
| SfaNI | GCATC | 6 cut(s) | 70, 181, 218, 811, 1479, 1903 |
| Sfr274I | CTCGAG | 1 cut(s) | 1077 |
| SinI | GGWCC | 7 cut(s) | 158, 196, 260, 289, 1208, 1514, 1823 |
| SlaI | CTCGAG | 1 cut(s) | 1077 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 610, 956, 1567, 1905 |
| SmlI | CTYRAG | 3 cut(s) | 1059, 1077, 1302 |
| SmoI | CTYRAG | 3 cut(s) | 1059, 1077, 1302 |
| SseBI | AGGCCT | 1 cut(s) | 1270 |
| SsiI | CCGC | 2 cut(s) | 148, 194 |
| SspMI | CTAG | 3 cut(s) | 915, 938, 1103 |
| StuI | AGGCCT | 1 cut(s) | 1270 |
| StyD4I | CCNGG | 2 cut(s) | 853, 1792 |
| StyI | CCWWGG | 1 cut(s) | 1856 |
| TaaI | ACNGT | 2 cut(s) | 226, 912 |
| TaiI | ACGT | 1 cut(s) | 1162 |
| TaqI | TCGA | 4 cut(s) | 634, 1078, 1391, 1697 |
| TatI | WGTACW | 3 cut(s) | 118, 214, 576 |
| TfiI | GAWTC | 3 cut(s) | 94, 304, 969 |
| Tru1I | TTAA | 8 cut(s) | 318, 410, 558, 690, 767, 863, 1001, 1497 |
| Tru9I | TTAA | 8 cut(s) | 318, 410, 558, 690, 767, 863, 1001, 1497 |
| TscAI | CASTG | 3 cut(s) | 684, 958, 1177 |
| TseFI | GTSAC | 1 cut(s) | 569 |
| TseI | GCWGC | 2 cut(s) | 458, 641 |
| Tsp45I | GTSAC | 1 cut(s) | 569 |
| TspDTI | ATGAA | 6 cut(s) | 665, 1235, 1383, 1418, 1796, 1923 |
| TspRI | CASTG | 3 cut(s) | 684, 958, 1177 |
| VpaK11BI | GGWCC | 7 cut(s) | 158, 196, 260, 289, 1208, 1514, 1823 |
| VspI | ATTAAT | 1 cut(s) | 1001 |
| XapI | RAATTY | 1 cut(s) | 1405 |
| XbaI | TCTAGA | 1 cut(s) | 1102 |
| XceI | RCATGY | 4 cut(s) | 395, 615, 929, 1554 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1001 |
| XhoI | CTCGAG | 1 cut(s) | 1077 |
| XmnI | GAANNNNTTC | 3 cut(s) | 431, 480, 1257 |
| XspI | CTAG | 3 cut(s) | 915, 938, 1103 |
| Zsp2I | ATGCAT | 1 cut(s) | 211 |