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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 6 cut(s) | 821, 1253, 1443, 1562, 1843, 1871 |
| AcoI | YGGCCR | 2 cut(s) | 1715, 2024 |
| AcsI | RAATTY | 4 cut(s) | 306, 317, 1281, 1699 |
| AcuI | CTGAAG | 3 cut(s) | 876, 1257, 1875 |
| AfaI | GTAC | 4 cut(s) | 198, 387, 826, 2011 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 568, 1968 |
| AflII | CTTAAG | 1 cut(s) | 248 |
| AflIII | ACRYGT | 2 cut(s) | 394, 725 |
| AhlI | ACTAGT | 1 cut(s) | 659 |
| AjnI | CCWGG | 2 cut(s) | 295, 967 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1989, 2021 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 147, 179 |
| AluBI | AGCT | 5 cut(s) | 230, 427, 1201, 1721, 1984 |
| AluI | AGCT | 5 cut(s) | 230, 427, 1201, 1721, 1984 |
| AlwI | GGATC | 6 cut(s) | 821, 1253, 1443, 1562, 1843, 1871 |
| Ama87I | CYCGRG | 2 cut(s) | 48, 238 |
| ApeKI | GCWGC | 1 cut(s) | 230 |
| ApoI | RAATTY | 4 cut(s) | 306, 317, 1281, 1699 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 594, 641 |
| AspS9I | GGNCC | 7 cut(s) | 263, 377, 537, 1292, 1601, 1866, 1910 |
| AsuHPI | GGTGA | 6 cut(s) | 168, 344, 356, 443, 892, 1775 |
| AvaI | CYCGRG | 2 cut(s) | 48, 238 |
| AvaII | GGWCC | 4 cut(s) | 263, 1292, 1601, 1866 |
| BalI | TGGCCA | 2 cut(s) | 1717, 2026 |
| BanII | GRGCYC | 1 cut(s) | 353 |
| BbsI | GAAGAC | 1 cut(s) | 357 |
| BbvI | GCAGC | 1 cut(s) | 217 |
| BccI | CCATC | 7 cut(s) | 223, 514, 830, 913, 1010, 1754, 1981 |
| BciT130I | CCWGG | 2 cut(s) | 297, 969 |
| BcuI | ACTAGT | 1 cut(s) | 659 |
| BfaI | CTAG | 5 cut(s) | 6, 258, 660, 798, 1205 |
| BfmI | CTRYAG | 3 cut(s) | 231, 870, 1707 |
| BfrI | CTTAAG | 1 cut(s) | 248 |
| BisI | GCNGC | 1 cut(s) | 231 |
| BlsI | GCNGC | 1 cut(s) | 232 |
| Bme1390I | CCNGG | 2 cut(s) | 297, 969 |
| Bme18I | GGWCC | 4 cut(s) | 263, 1292, 1601, 1866 |
| BmeT110I | CYCGRG | 2 cut(s) | 48, 238 |
| BmgT120I | GGNCC | 7 cut(s) | 263, 377, 537, 1292, 1601, 1866, 1910 |
| BmiI | GGNNCC | 3 cut(s) | 294, 1293, 1911 |
| BmrFI | CCNGG | 2 cut(s) | 297, 969 |
| BmsI | GCATC | 8 cut(s) | 74, 196, 925, 1041, 1327, 1781, 1967, 1990 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1864 |
| BpiI | GAAGAC | 1 cut(s) | 357 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 13, 45 |
| BpuEI | CTTGAG | 3 cut(s) | 1371, 1477, 1855 |
| Bsa29I | ATCGAT | 1 cut(s) | 518 |
| BsaAI | YACGTR | 1 cut(s) | 824 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1881 |
| BsaJI | CCNNGG | 2 cut(s) | 1287, 1905 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 585, 615 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 568, 1968 |
| Bse1I | ACTGG | 2 cut(s) | 845, 1528 |
| Bse3DI | GCAATG | 1 cut(s) | 1308 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1881 |
| BseBI | CCWGG | 2 cut(s) | 297, 969 |
| BseCI | ATCGAT | 1 cut(s) | 518 |
| BseDI | CCNNGG | 2 cut(s) | 1287, 1905 |
| BseGI | GGATG | 9 cut(s) | 162, 530, 645, 841, 908, 1772, 1831, 1958, 1976 |
| BseJI | GATNNNNATC | 1 cut(s) | 1881 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 568, 1968 |
| BseMI | GCAATG | 1 cut(s) | 1308 |
| BseMII | CTCAG | 2 cut(s) | 366, 1437 |
| BseNI | ACTGG | 2 cut(s) | 845, 1528 |
| BseRI | GAGGAG | 2 cut(s) | 500, 1163 |
| BseXI | GCAGC | 1 cut(s) | 217 |
| BseYI | CCCAGC | 1 cut(s) | 1245 |
| BshVI | ATCGAT | 1 cut(s) | 518 |
| BsiHKCI | CYCGRG | 2 cut(s) | 48, 238 |
| BslFI | GGGAC | 3 cut(s) | 1305, 1854, 1934 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 568, 1968 |
| BsmFI | GGGAC | 3 cut(s) | 1305, 1854, 1934 |
| BsmI | GAATGC | 2 cut(s) | 1016, 1558 |
| BsoBI | CYCGRG | 2 cut(s) | 48, 238 |
| Bsp1286I | GDGCHC | 1 cut(s) | 353 |
| Bsp143I | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| BspCNI | CTCAG | 2 cut(s) | 365, 1438 |
| BspDI | ATCGAT | 1 cut(s) | 518 |
| BspHI | TCATGA | 1 cut(s) | 1642 |
| BspLI | GGNNCC | 3 cut(s) | 294, 1293, 1911 |
| BspMAI | CTGCAG | 1 cut(s) | 235 |
| BspPI | GGATC | 6 cut(s) | 821, 1253, 1443, 1562, 1843, 1871 |
| BspTI | CTTAAG | 1 cut(s) | 248 |
| BsrDI | GCAATG | 1 cut(s) | 1308 |
| BsrI | ACTGG | 2 cut(s) | 845, 1528 |
| BssECI | CCNNGG | 2 cut(s) | 1287, 1905 |
| BssMI | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| BssT1I | CCWWGG | 2 cut(s) | 1287, 1905 |
| Bst2UI | CCWGG | 2 cut(s) | 297, 969 |
| Bst4CI | ACNGT | 1 cut(s) | 776 |
| Bst6I | CTCTTC | 3 cut(s) | 985, 1140, 1260 |
| BstAFI | CTTAAG | 1 cut(s) | 248 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1409 |
| BstBAI | YACGTR | 1 cut(s) | 824 |
| BstC8I | GCNNGC | 4 cut(s) | 1199, 1410, 1719, 2028 |
| BstDEI | CTNAG | 7 cut(s) | 352, 609, 952, 977, 1029, 1047, 1446 |
| BstF5I | GGATG | 9 cut(s) | 162, 530, 645, 841, 908, 1772, 1831, 1958, 1976 |
| BstKTI | GATC | 8 cut(s) | 816, 821, 1261, 1438, 1570, 1838, 1879, 1885 |
| BstMBI | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 11, 53, 62, 674, 752, 1409, 1718, 2027 |
| BstNI | CCWGG | 2 cut(s) | 297, 969 |
| BstNSI | RCATGY | 6 cut(s) | 47, 398, 729, 1043, 1075, 1385 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1864 |
| BstSCI | CCNGG | 2 cut(s) | 295, 967 |
| BstSFI | CTRYAG | 3 cut(s) | 231, 870, 1707 |
| BstV1I | GCAGC | 1 cut(s) | 217 |
| BstV2I | GAAGAC | 1 cut(s) | 357 |
| Bsu15I | ATCGAT | 1 cut(s) | 518 |
| BsuTUI | ATCGAT | 1 cut(s) | 518 |
| BtgZI | GCGATG | 1 cut(s) | 156 |
| BtsCI | GGATG | 9 cut(s) | 162, 530, 645, 841, 908, 1772, 1831, 1958, 1976 |
| BtsIMutI | CAGTG | 3 cut(s) | 688, 781, 852 |
| Cac8I | GCNNGC | 4 cut(s) | 1199, 1410, 1719, 2028 |
| CciI | TCATGA | 1 cut(s) | 1642 |
| Cfr13I | GGNCC | 7 cut(s) | 263, 377, 537, 1292, 1601, 1866, 1910 |
| ClaI | ATCGAT | 1 cut(s) | 518 |
| CseI | GACGC | 1 cut(s) | 1159 |
| Csp6I | GTAC | 4 cut(s) | 197, 386, 825, 2010 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 174, 209 |
| CviQI | GTAC | 4 cut(s) | 197, 386, 825, 2010 |
| DdeI | CTNAG | 7 cut(s) | 352, 609, 952, 977, 1029, 1047, 1446 |
| DpnI | GATC | 8 cut(s) | 815, 820, 1260, 1437, 1569, 1837, 1878, 1884 |
| DpnII | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| DraI | TTTAAA | 1 cut(s) | 514 |
| EaeI | YGGCCR | 2 cut(s) | 1715, 2024 |
| Eam1104I | CTCTTC | 3 cut(s) | 985, 1140, 1260 |
| EarI | CTCTTC | 3 cut(s) | 985, 1140, 1260 |
| Eco130I | CCWWGG | 2 cut(s) | 1287, 1905 |
| Eco147I | AGGCCT | 2 cut(s) | 1354, 1663 |
| Eco24I | GRGCYC | 1 cut(s) | 353 |
| Eco32I | GATATC | 2 cut(s) | 1321, 1746 |
| Eco47I | GGWCC | 4 cut(s) | 263, 1292, 1601, 1866 |
| Eco57I | CTGAAG | 3 cut(s) | 876, 1257, 1875 |
| Eco88I | CYCGRG | 2 cut(s) | 48, 238 |
| EcoO109I | RGGNCCY | 3 cut(s) | 537, 1292, 1910 |
| EcoRI | GAATTC | 2 cut(s) | 306, 1699 |
| EcoRII | CCWGG | 2 cut(s) | 295, 967 |
| EcoRV | GATATC | 2 cut(s) | 1321, 1746 |
| EcoT14I | CCWWGG | 2 cut(s) | 1287, 1905 |
| EcoT22I | ATGCAT | 1 cut(s) | 1774 |
| EcoT38I | GRGCYC | 1 cut(s) | 353 |
| ErhI | CCWWGG | 2 cut(s) | 1287, 1905 |
| FaqI | GGGAC | 3 cut(s) | 1305, 1854, 1934 |
| Fnu4HI | GCNGC | 1 cut(s) | 231 |
| FokI | GGATG | 9 cut(s) | 149, 537, 632, 848, 895, 1759, 1838, 1945, 1963 |
| FriOI | GRGCYC | 1 cut(s) | 353 |
| Fsp4HI | GCNGC | 1 cut(s) | 231 |
| FspBI | CTAG | 5 cut(s) | 6, 258, 660, 798, 1205 |
| GluI | GCNGC | 1 cut(s) | 231 |
| GsaI | CCCAGC | 1 cut(s) | 1249 |
| HgaI | GACGC | 1 cut(s) | 1159 |
| HincII | GTYRAC | 2 cut(s) | 1174, 1792 |
| HindII | GTYRAC | 2 cut(s) | 1174, 1792 |
| HindIII | AAGCTT | 1 cut(s) | 425 |
| HinfI | GANTC | 6 cut(s) | 148, 224, 600, 1129, 1268, 1639 |
| HphI | GGTGA | 6 cut(s) | 168, 344, 356, 443, 892, 1775 |
| Hpy166II | GTNNAC | 5 cut(s) | 335, 842, 850, 1174, 1792 |
| Hpy188I | TCNGA | 4 cut(s) | 818, 1447, 1750, 1981 |
| Hpy8I | GTNNAC | 5 cut(s) | 335, 842, 850, 1174, 1792 |
| HpyAV | CCTTC | 8 cut(s) | 189, 493, 795, 1232, 1482, 1541, 1791, 1850 |
| HpyCH4III | ACNGT | 1 cut(s) | 776 |
| HpyCH4IV | ACGT | 3 cut(s) | 823, 1552, 1861 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 11, 53, 62, 674, 752, 1409, 1718, 2027 |
| HpyF3I | CTNAG | 7 cut(s) | 352, 609, 952, 977, 1029, 1047, 1446 |
| HpySE526I | ACGT | 3 cut(s) | 823, 1552, 1861 |
| Kzo9I | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| LmnI | GCTCC | 1 cut(s) | 292 |
| Lsp1109I | GCAGC | 1 cut(s) | 217 |
| LweI | GCATC | 8 cut(s) | 74, 196, 925, 1041, 1327, 1781, 1967, 1990 |
| MaeI | CTAG | 5 cut(s) | 6, 258, 660, 798, 1205 |
| MaeII | ACGT | 3 cut(s) | 823, 1552, 1861 |
| MaeIII | GTNAC | 1 cut(s) | 403 |
| MalI | GATC | 8 cut(s) | 815, 820, 1260, 1437, 1569, 1837, 1878, 1884 |
| MboI | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| MfeI | CAATTG | 1 cut(s) | 1668 |
| MhlI | GDGCHC | 1 cut(s) | 353 |
| MlsI | TGGCCA | 2 cut(s) | 1717, 2026 |
| MluNI | TGGCCA | 2 cut(s) | 1717, 2026 |
| MlyI | GAGTC | 3 cut(s) | 157, 594, 1277 |
| MmeI | TCCRAC | 4 cut(s) | 190, 290, 628, 1803 |
| Mox20I | TGGCCA | 2 cut(s) | 1717, 2026 |
| Mph1103I | ATGCAT | 1 cut(s) | 1774 |
| MroXI | GAANNNNTTC | 2 cut(s) | 594, 641 |
| MscI | TGGCCA | 2 cut(s) | 1717, 2026 |
| MseI | TTAA | 6 cut(s) | 131, 249, 513, 672, 804, 1068 |
| MslI | CAYNNNNRTG | 3 cut(s) | 724, 1563, 1654 |
| Msp20I | TGGCCA | 2 cut(s) | 1717, 2026 |
| MspA1I | CMGCKG | 1 cut(s) | 230 |
| MspCI | CTTAAG | 1 cut(s) | 248 |
| MspR9I | CCNGG | 2 cut(s) | 297, 969 |
| MunI | CAATTG | 1 cut(s) | 1668 |
| Mva1269I | GAATGC | 2 cut(s) | 1016, 1558 |
| MvaI | CCWGG | 2 cut(s) | 297, 969 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 11, 53, 62, 674, 752, 1409, 1718, 2027 |
| NdeII | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| NlaIV | GGNNCC | 3 cut(s) | 294, 1293, 1911 |
| NsiI | ATGCAT | 1 cut(s) | 1774 |
| NspI | RCATGY | 6 cut(s) | 47, 398, 729, 1043, 1075, 1385 |
| PagI | TCATGA | 1 cut(s) | 1642 |
| PceI | AGGCCT | 2 cut(s) | 1354, 1663 |
| PciI | ACATGT | 2 cut(s) | 394, 725 |
| PctI | GAATGC | 2 cut(s) | 1016, 1558 |
| PdmI | GAANNNNTTC | 2 cut(s) | 594, 641 |
| PfeI | GAWTC | 3 cut(s) | 224, 1129, 1639 |
| PkrI | GCNGC | 1 cut(s) | 232 |
| PleI | GAGTC | 3 cut(s) | 156, 594, 1276 |
| PpsI | GAGTC | 3 cut(s) | 156, 594, 1276 |
| Ppu21I | YACGTR | 1 cut(s) | 824 |
| PpuMI | RGGWCCY | 1 cut(s) | 1292 |
| PscI | ACATGT | 2 cut(s) | 394, 725 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1864 |
| Psp5II | RGGWCCY | 1 cut(s) | 1292 |
| Psp6I | CCWGG | 2 cut(s) | 295, 967 |
| PspFI | CCCAGC | 1 cut(s) | 1245 |
| PspGI | CCWGG | 2 cut(s) | 295, 967 |
| PspN4I | GGNNCC | 3 cut(s) | 294, 1293, 1911 |
| PspPI | GGNCC | 7 cut(s) | 263, 377, 537, 1292, 1601, 1866, 1910 |
| PspPPI | RGGWCCY | 1 cut(s) | 1292 |
| PstI | CTGCAG | 1 cut(s) | 235 |
| PvuII | CAGCTG | 1 cut(s) | 230 |
| RsaI | GTAC | 4 cut(s) | 198, 387, 826, 2011 |
| RsaNI | GTAC | 4 cut(s) | 197, 386, 825, 2010 |
| RseI | CAYNNNNRTG | 3 cut(s) | 724, 1563, 1654 |
| SaqAI | TTAA | 6 cut(s) | 131, 249, 513, 672, 804, 1068 |
| SatI | GCNGC | 1 cut(s) | 231 |
| Sau3AI | GATC | 8 cut(s) | 813, 818, 1258, 1435, 1567, 1835, 1876, 1882 |
| Sau96I | GGNCC | 7 cut(s) | 263, 377, 537, 1292, 1601, 1866, 1910 |
| SchI | GAGTC | 3 cut(s) | 157, 594, 1277 |
| ScrFI | CCNGG | 2 cut(s) | 297, 969 |
| SduI | GDGCHC | 1 cut(s) | 353 |
| SfaNI | GCATC | 8 cut(s) | 74, 196, 925, 1041, 1327, 1781, 1967, 1990 |
| SfcI | CTRYAG | 3 cut(s) | 231, 870, 1707 |
| SinI | GGWCC | 4 cut(s) | 263, 1292, 1601, 1866 |
| SmiI | ATTTAAAT | 1 cut(s) | 514 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 724, 1563, 1654 |
| SmlI | CTYRAG | 4 cut(s) | 248, 1386, 1492, 1870 |
| SmoI | CTYRAG | 4 cut(s) | 248, 1386, 1492, 1870 |
| SpeI | ACTAGT | 1 cut(s) | 659 |
| SseBI | AGGCCT | 2 cut(s) | 1354, 1663 |
| SspMI | CTAG | 5 cut(s) | 6, 258, 660, 798, 1205 |
| StuI | AGGCCT | 2 cut(s) | 1354, 1663 |
| StyD4I | CCNGG | 2 cut(s) | 295, 967 |
| StyI | CCWWGG | 2 cut(s) | 1287, 1905 |
| SwaI | ATTTAAAT | 1 cut(s) | 514 |
| TaaI | ACNGT | 1 cut(s) | 776 |
| TaiI | ACGT | 3 cut(s) | 826, 1555, 1864 |
| TaqI | TCGA | 6 cut(s) | 304, 518, 598, 748, 1475, 1784 |
| TfiI | GAWTC | 3 cut(s) | 224, 1129, 1639 |
| Tru1I | TTAA | 6 cut(s) | 131, 249, 513, 672, 804, 1068 |
| Tru9I | TTAA | 6 cut(s) | 131, 249, 513, 672, 804, 1068 |
| TscAI | CASTG | 3 cut(s) | 688, 781, 859 |
| TseI | GCWGC | 1 cut(s) | 230 |
| TspGWI | ACGGA | 1 cut(s) | 638 |
| TspRI | CASTG | 3 cut(s) | 688, 781, 859 |
| Vha464I | CTTAAG | 1 cut(s) | 248 |
| VpaK11BI | GGWCC | 4 cut(s) | 263, 1292, 1601, 1866 |
| XapI | RAATTY | 4 cut(s) | 306, 317, 1281, 1699 |
| XbaI | TCTAGA | 1 cut(s) | 797 |
| XceI | RCATGY | 6 cut(s) | 47, 398, 729, 1043, 1075, 1385 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 190, 1009 |
| XmnI | GAANNNNTTC | 2 cut(s) | 594, 641 |
| XspI | CTAG | 5 cut(s) | 6, 258, 660, 798, 1205 |
| Zsp2I | ATGCAT | 1 cut(s) | 1774 |