source UniProtKB
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 234 |
| AcsI | RAATTY | 2 cut(s) | 306, 317 |
| AfaI | GTAC | 3 cut(s) | 198, 387, 399 |
| AflII | CTTAAG | 1 cut(s) | 248 |
| AflIII | ACRYGT | 1 cut(s) | 394 |
| AgsI | TTSAA | 1 cut(s) | 317 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 147, 179 |
| AluBI | AGCT | 3 cut(s) | 230, 351, 356 |
| AluI | AGCT | 3 cut(s) | 230, 351, 356 |
| Alw21I | GWGCWC | 1 cut(s) | 353 |
| Ama87I | CYCGRG | 2 cut(s) | 48, 238 |
| AoxI | GGCC | 1 cut(s) | 376 |
| ApoI | RAATTY | 2 cut(s) | 306, 317 |
| AspS9I | GGNCC | 2 cut(s) | 263, 377 |
| AsuHPI | GGTGA | 4 cut(s) | 20, 168, 344, 356 |
| AvaI | CYCGRG | 2 cut(s) | 48, 238 |
| AvaII | GGWCC | 1 cut(s) | 263 |
| BanII | GRGCYC | 1 cut(s) | 353 |
| BbsI | GAAGAC | 1 cut(s) | 357 |
| Bbv12I | GWGCWC | 1 cut(s) | 353 |
| BfaI | CTAG | 2 cut(s) | 258, 357 |
| BfmI | CTRYAG | 1 cut(s) | 231 |
| BfrI | CTTAAG | 1 cut(s) | 248 |
| BlpI | GCTNAGC | 1 cut(s) | 352 |
| Bme18I | GGWCC | 1 cut(s) | 263 |
| BmeT110I | CYCGRG | 2 cut(s) | 48, 238 |
| BmgT120I | GGNCC | 2 cut(s) | 263, 377 |
| BmiI | GGNNCC | 2 cut(s) | 264, 294 |
| BmsI | GCATC | 2 cut(s) | 74, 196 |
| BpiI | GAAGAC | 1 cut(s) | 357 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 13, 45 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 352 |
| BsaAI | YACGTR | 1 cut(s) | 397 |
| BseGI | GGATG | 1 cut(s) | 162 |
| BseMII | CTCAG | 1 cut(s) | 366 |
| BshFI | GGCC | 1 cut(s) | 378 |
| BsiHKAI | GWGCWC | 1 cut(s) | 353 |
| BsiHKCI | CYCGRG | 2 cut(s) | 48, 238 |
| BsnI | GGCC | 1 cut(s) | 378 |
| BsoBI | CYCGRG | 2 cut(s) | 48, 238 |
| Bsp1286I | GDGCHC | 1 cut(s) | 353 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 352 |
| BspANI | GGCC | 1 cut(s) | 378 |
| BspCNI | CTCAG | 1 cut(s) | 365 |
| BspLI | GGNNCC | 2 cut(s) | 264, 294 |
| BspTI | CTTAAG | 1 cut(s) | 248 |
| Bst4CI | ACNGT | 1 cut(s) | 216 |
| BstAFI | CTTAAG | 1 cut(s) | 248 |
| BstBAI | YACGTR | 1 cut(s) | 397 |
| BstDEI | CTNAG | 1 cut(s) | 352 |
| BstF5I | GGATG | 1 cut(s) | 162 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 11, 62 |
| BstSFI | CTRYAG | 1 cut(s) | 231 |
| BstV2I | GAAGAC | 1 cut(s) | 357 |
| BsuRI | GGCC | 1 cut(s) | 378 |
| BtsCI | GGATG | 1 cut(s) | 162 |
| Cfr13I | GGNCC | 2 cut(s) | 263, 377 |
| Csp6I | GTAC | 3 cut(s) | 197, 386, 398 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 174, 209 |
| CviAII | CATG | 4 cut(s) | 80, 138, 311, 339 |
| CviJI | RGCY | 7 cut(s) | 5, 56, 230, 295, 351, 356, 378 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 56, 230, 295, 351, 356, 378 |
| CviQI | GTAC | 3 cut(s) | 197, 386, 398 |
| DdeI | CTNAG | 1 cut(s) | 352 |
| Ecl136II | GAGCTC | 1 cut(s) | 351 |
| Eco24I | GRGCYC | 1 cut(s) | 353 |
| Eco47I | GGWCC | 1 cut(s) | 263 |
| Eco53kI | GAGCTC | 1 cut(s) | 351 |
| Eco88I | CYCGRG | 2 cut(s) | 48, 238 |
| EcoICRI | GAGCTC | 1 cut(s) | 351 |
| EcoRI | GAATTC | 1 cut(s) | 306 |
| EcoT38I | GRGCYC | 1 cut(s) | 353 |
| FaeI | CATG | 4 cut(s) | 83, 141, 314, 342 |
| FaiI | YATR | 8 cut(s) | 33, 45, 81, 98, 118, 139, 312, 340 |
| FatI | CATG | 4 cut(s) | 79, 137, 310, 338 |
| FblI | GTMKAC | 1 cut(s) | 234 |
| FokI | GGATG | 1 cut(s) | 149 |
| FriOI | GRGCYC | 1 cut(s) | 353 |
| FspBI | CTAG | 2 cut(s) | 258, 357 |
| HaeIII | GGCC | 1 cut(s) | 378 |
| Hin1II | CATG | 4 cut(s) | 83, 141, 314, 342 |
| HinfI | GANTC | 1 cut(s) | 148 |
| HphI | GGTGA | 4 cut(s) | 20, 168, 344, 356 |
| Hpy166II | GTNNAC | 2 cut(s) | 235, 335 |
| Hpy188III | TCNNGA | 4 cut(s) | 50, 110, 122, 152 |
| Hpy8I | GTNNAC | 2 cut(s) | 235, 335 |
| HpyAV | CCTTC | 1 cut(s) | 189 |
| HpyCH4III | ACNGT | 1 cut(s) | 216 |
| HpyCH4IV | ACGT | 1 cut(s) | 396 |
| HpyCH4V | TGCA | 2 cut(s) | 14, 209 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 11, 62 |
| HpyF3I | CTNAG | 1 cut(s) | 352 |
| HpySE526I | ACGT | 1 cut(s) | 396 |
| Hsp92II | CATG | 4 cut(s) | 83, 141, 314, 342 |
| LmnI | GCTCC | 1 cut(s) | 292 |
| LpnPI | CCDG | 4 cut(s) | 123, 165, 309, 414 |
| LweI | GCATC | 2 cut(s) | 74, 196 |
| MaeI | CTAG | 2 cut(s) | 258, 357 |
| MaeII | ACGT | 1 cut(s) | 396 |
| MaeIII | GTNAC | 2 cut(s) | 216, 403 |
| MboII | GAAGA | 2 cut(s) | 215, 357 |
| MhlI | GDGCHC | 1 cut(s) | 353 |
| MluCI | AATT | 2 cut(s) | 306, 317 |
| MlyI | GAGTC | 1 cut(s) | 157 |
| MmeI | TCCRAC | 2 cut(s) | 190, 290 |
| MnlI | CCTC | 2 cut(s) | 30, 236 |
| MseI | TTAA | 2 cut(s) | 131, 249 |
| MspCI | CTTAAG | 1 cut(s) | 248 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 11, 62 |
| NlaIII | CATG | 4 cut(s) | 83, 141, 314, 342 |
| NlaIV | GGNNCC | 2 cut(s) | 264, 294 |
| NmuCI | GTSAC | 1 cut(s) | 216 |
| PleI | GAGTC | 1 cut(s) | 156 |
| PpsI | GAGTC | 1 cut(s) | 156 |
| Ppu21I | YACGTR | 1 cut(s) | 397 |
| Psp124BI | GAGCTC | 1 cut(s) | 353 |
| PspN4I | GGNNCC | 2 cut(s) | 264, 294 |
| PspPI | GGNCC | 2 cut(s) | 263, 377 |
| RsaI | GTAC | 3 cut(s) | 198, 387, 399 |
| RsaNI | GTAC | 3 cut(s) | 197, 386, 398 |
| SacI | GAGCTC | 1 cut(s) | 353 |
| SaqAI | TTAA | 2 cut(s) | 131, 249 |
| Sau96I | GGNCC | 2 cut(s) | 263, 377 |
| SchI | GAGTC | 1 cut(s) | 157 |
| SduI | GDGCHC | 1 cut(s) | 353 |
| SetI | ASST | 9 cut(s) | 75, 160, 181, 232, 334, 353, 358, 399, 403 |
| SfaNI | GCATC | 2 cut(s) | 74, 196 |
| SfcI | CTRYAG | 1 cut(s) | 231 |
| SinI | GGWCC | 1 cut(s) | 263 |
| SmlI | CTYRAG | 1 cut(s) | 248 |
| SmoI | CTYRAG | 1 cut(s) | 248 |
| Sse9I | AATT | 2 cut(s) | 306, 317 |
| SspMI | CTAG | 2 cut(s) | 258, 357 |
| SstI | GAGCTC | 1 cut(s) | 353 |
| TaaI | ACNGT | 1 cut(s) | 216 |
| TaiI | ACGT | 1 cut(s) | 399 |
| TaqI | TCGA | 1 cut(s) | 304 |
| TasI | AATT | 2 cut(s) | 306, 317 |
| Tru1I | TTAA | 2 cut(s) | 131, 249 |
| Tru9I | TTAA | 2 cut(s) | 131, 249 |
| TseFI | GTSAC | 1 cut(s) | 216 |
| Tsp45I | GTSAC | 1 cut(s) | 216 |
| TspDTI | ATGAA | 2 cut(s) | 290, 299 |
| Vha464I | CTTAAG | 1 cut(s) | 248 |
| VpaK11BI | GGWCC | 1 cut(s) | 263 |
| XapI | RAATTY | 2 cut(s) | 306, 317 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 190 |
| XmiI | GTMKAC | 1 cut(s) | 234 |
| XspI | CTAG | 2 cut(s) | 258, 357 |