DNA-directed 5'-3' RNA polymerase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 50, 52 |
| AciI | CCGC | 2 cut(s) | 4, 52 |
| AclWI | GGATC | 4 cut(s) | 164, 177, 266, 806 |
| AfaI | GTAC | 2 cut(s) | 946, 968 |
| AgsI | TTSAA | 7 cut(s) | 59, 238, 318, 419, 427, 551, 665 |
| AluBI | AGCT | 1 cut(s) | 893 |
| AluI | AGCT | 1 cut(s) | 893 |
| AlwI | GGATC | 4 cut(s) | 164, 177, 266, 806 |
| AoxI | GGCC | 2 cut(s) | 620, 956 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 788, 820 |
| AspLEI | GCGC | 1 cut(s) | 52 |
| AspS9I | GGNCC | 2 cut(s) | 266, 714 |
| AsuHPI | GGTGA | 1 cut(s) | 689 |
| AvaII | GGWCC | 2 cut(s) | 266, 714 |
| BaeGI | GKGCMC | 1 cut(s) | 840 |
| BamHI | GGATCC | 1 cut(s) | 169 |
| BauI | CACGAG | 1 cut(s) | 276 |
| BccI | CCATC | 4 cut(s) | 560, 674, 763, 837 |
| BciVI | GTATCC | 1 cut(s) | 111 |
| BclI | TGATCA | 1 cut(s) | 733 |
| BfaI | CTAG | 4 cut(s) | 263, 594, 800, 909 |
| BfmI | CTRYAG | 1 cut(s) | 249 |
| BfuI | GTATCC | 1 cut(s) | 111 |
| BisI | GCNGC | 1 cut(s) | 53 |
| BlsI | GCNGC | 1 cut(s) | 54 |
| Bme18I | GGWCC | 2 cut(s) | 266, 714 |
| BmgT120I | GGNCC | 2 cut(s) | 266, 714 |
| BmiI | GGNNCC | 1 cut(s) | 171 |
| BmsI | GCATC | 2 cut(s) | 82, 235 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 919, 951 |
| BpuEI | CTTGAG | 1 cut(s) | 921 |
| BsaJI | CCNNGG | 1 cut(s) | 841 |
| Bse1I | ACTGG | 1 cut(s) | 658 |
| BseDI | CCNNGG | 1 cut(s) | 841 |
| BseGI | GGATG | 1 cut(s) | 736 |
| BseMII | CTCAG | 1 cut(s) | 917 |
| BseNI | ACTGG | 1 cut(s) | 658 |
| BseSI | GKGCMC | 1 cut(s) | 840 |
| Bsh1236I | CGCG | 2 cut(s) | 50, 52 |
| BshFI | GGCC | 2 cut(s) | 622, 958 |
| BslFI | GGGAC | 1 cut(s) | 296 |
| BsmFI | GGGAC | 1 cut(s) | 296 |
| BsmI | GAATGC | 2 cut(s) | 499, 857 |
| BsnI | GGCC | 2 cut(s) | 622, 958 |
| Bsp1286I | GDGCHC | 1 cut(s) | 840 |
| Bsp143I | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| BspACI | CCGC | 2 cut(s) | 4, 52 |
| BspANI | GGCC | 2 cut(s) | 622, 958 |
| BspCNI | CTCAG | 1 cut(s) | 918 |
| BspFNI | CGCG | 2 cut(s) | 50, 52 |
| BspHI | TCATGA | 1 cut(s) | 847 |
| BspLI | GGNNCC | 1 cut(s) | 171 |
| BspPI | GGATC | 4 cut(s) | 164, 177, 266, 806 |
| BsrI | ACTGG | 1 cut(s) | 658 |
| BssECI | CCNNGG | 1 cut(s) | 841 |
| BssMI | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| BssSI | CACGAG | 1 cut(s) | 276 |
| BssT1I | CCWWGG | 1 cut(s) | 841 |
| Bst2BI | CACGAG | 1 cut(s) | 276 |
| Bst4CI | ACNGT | 2 cut(s) | 491, 949 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 632 |
| BstDEI | CTNAG | 3 cut(s) | 361, 510, 926 |
| BstF5I | GGATG | 1 cut(s) | 736 |
| BstFNI | CGCG | 2 cut(s) | 50, 52 |
| BstHHI | GCGC | 1 cut(s) | 52 |
| BstKTI | GATC | 5 cut(s) | 172, 274, 458, 736, 814 |
| BstMBI | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 131, 636 |
| BstNSI | RCATGY | 1 cut(s) | 125 |
| BstSFI | CTRYAG | 1 cut(s) | 249 |
| BstSLI | GKGCMC | 1 cut(s) | 840 |
| BstUI | CGCG | 2 cut(s) | 50, 52 |
| BstX2I | RGATCY | 2 cut(s) | 169, 811 |
| BstYI | RGATCY | 2 cut(s) | 169, 811 |
| BsuI | GTATCC | 1 cut(s) | 111 |
| BsuRI | GGCC | 2 cut(s) | 622, 958 |
| BtsCI | GGATG | 1 cut(s) | 736 |
| BtsI | GCAGTG | 1 cut(s) | 568 |
| BtsIMutI | CAGTG | 3 cut(s) | 496, 568, 923 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 632 |
| CciI | TCATGA | 1 cut(s) | 847 |
| CfoI | GCGC | 1 cut(s) | 52 |
| Cfr13I | GGNCC | 2 cut(s) | 266, 714 |
| Csp6I | GTAC | 2 cut(s) | 945, 967 |
| CviAII | CATG | 5 cut(s) | 122, 479, 833, 848, 954 |
| CviJI | RGCY | 5 cut(s) | 55, 622, 893, 935, 958 |
| CviKI_1 | RGCY | 5 cut(s) | 55, 622, 893, 935, 958 |
| CviQI | GTAC | 2 cut(s) | 945, 967 |
| DdeI | CTNAG | 3 cut(s) | 361, 510, 926 |
| DpnI | GATC | 5 cut(s) | 171, 273, 457, 735, 813 |
| DpnII | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| Eco130I | CCWWGG | 1 cut(s) | 841 |
| Eco32I | GATATC | 1 cut(s) | 99 |
| Eco47I | GGWCC | 2 cut(s) | 266, 714 |
| EcoO109I | RGGNCCY | 1 cut(s) | 266 |
| EcoRV | GATATC | 1 cut(s) | 99 |
| EcoT14I | CCWWGG | 1 cut(s) | 841 |
| ErhI | CCWWGG | 1 cut(s) | 841 |
| FaeI | CATG | 5 cut(s) | 125, 482, 836, 851, 957 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 33 |
| FaqI | GGGAC | 1 cut(s) | 296 |
| FatI | CATG | 5 cut(s) | 121, 478, 832, 847, 953 |
| FbaI | TGATCA | 1 cut(s) | 733 |
| Fnu4HI | GCNGC | 1 cut(s) | 53 |
| FokI | GGATG | 1 cut(s) | 743 |
| Fsp4HI | GCNGC | 1 cut(s) | 53 |
| FspBI | CTAG | 4 cut(s) | 263, 594, 800, 909 |
| GlaI | GCGC | 1 cut(s) | 51 |
| GluI | GCNGC | 1 cut(s) | 53 |
| HaeIII | GGCC | 2 cut(s) | 622, 958 |
| HhaI | GCGC | 1 cut(s) | 52 |
| Hin1II | CATG | 5 cut(s) | 125, 482, 836, 851, 957 |
| Hin6I | GCGC | 1 cut(s) | 50 |
| HinP1I | GCGC | 1 cut(s) | 50 |
| HinfI | GANTC | 2 cut(s) | 744, 803 |
| HphI | GGTGA | 1 cut(s) | 689 |
| Hpy188I | TCNGA | 5 cut(s) | 109, 154, 460, 610, 738 |
| Hpy188III | TCNNGA | 4 cut(s) | 89, 173, 269, 848 |
| HpyAV | CCTTC | 4 cut(s) | 139, 240, 324, 698 |
| HpyCH4III | ACNGT | 2 cut(s) | 491, 949 |
| HpyCH4V | TGCA | 2 cut(s) | 788, 953 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 131, 636 |
| HpyF3I | CTNAG | 3 cut(s) | 361, 510, 926 |
| Hsp92II | CATG | 5 cut(s) | 125, 482, 836, 851, 957 |
| HspAI | GCGC | 1 cut(s) | 50 |
| Ksp22I | TGATCA | 1 cut(s) | 733 |
| Kzo9I | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| LmnI | GCTCC | 1 cut(s) | 305 |
| LpnPI | CCDG | 5 cut(s) | 186, 282, 639, 660, 841 |
| LweI | GCATC | 2 cut(s) | 82, 235 |
| MaeI | CTAG | 4 cut(s) | 263, 594, 800, 909 |
| MaeIII | GTNAC | 2 cut(s) | 485, 721 |
| MalI | GATC | 5 cut(s) | 171, 273, 457, 735, 813 |
| MboI | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| MboII | GAAGA | 6 cut(s) | 24, 71, 74, 151, 154, 785 |
| MflI | RGATCY | 2 cut(s) | 169, 811 |
| MhlI | GDGCHC | 1 cut(s) | 840 |
| MluCI | AATT | 2 cut(s) | 65, 749 |
| MlyI | GAGTC | 1 cut(s) | 812 |
| MmeI | TCCRAC | 1 cut(s) | 322 |
| MnlI | CCTC | 9 cut(s) | 63, 148, 160, 229, 272, 284, 596, 612, 877 |
| MseI | TTAA | 1 cut(s) | 755 |
| MslI | CAYNNNNRTG | 2 cut(s) | 126, 561 |
| Mva1269I | GAATGC | 2 cut(s) | 499, 857 |
| MvnI | CGCG | 2 cut(s) | 50, 52 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 131, 636 |
| NdeII | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| NlaIII | CATG | 5 cut(s) | 125, 482, 836, 851, 957 |
| NlaIV | GGNNCC | 1 cut(s) | 171 |
| NspI | RCATGY | 1 cut(s) | 125 |
| PagI | TCATGA | 1 cut(s) | 847 |
| PctI | GAATGC | 2 cut(s) | 499, 857 |
| PfeI | GAWTC | 1 cut(s) | 744 |
| PkrI | GCNGC | 1 cut(s) | 54 |
| PleI | GAGTC | 1 cut(s) | 811 |
| PpsI | GAGTC | 1 cut(s) | 811 |
| PpuMI | RGGWCCY | 1 cut(s) | 266 |
| Psp5II | RGGWCCY | 1 cut(s) | 266 |
| PspN4I | GGNNCC | 1 cut(s) | 171 |
| PspPI | GGNCC | 2 cut(s) | 266, 714 |
| PspPPI | RGGWCCY | 1 cut(s) | 266 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 243, 275 |
| PsuI | RGATCY | 2 cut(s) | 169, 811 |
| RsaI | GTAC | 2 cut(s) | 946, 968 |
| RsaNI | GTAC | 2 cut(s) | 945, 967 |
| RseI | CAYNNNNRTG | 2 cut(s) | 126, 561 |
| SaqAI | TTAA | 1 cut(s) | 755 |
| SatI | GCNGC | 1 cut(s) | 53 |
| Sau3AI | GATC | 5 cut(s) | 169, 271, 455, 733, 811 |
| Sau96I | GGNCC | 2 cut(s) | 266, 714 |
| SchI | GAGTC | 1 cut(s) | 812 |
| SduI | GDGCHC | 1 cut(s) | 840 |
| SetI | ASST | 7 cut(s) | 107, 232, 268, 295, 607, 709, 895 |
| SfaNI | GCATC | 2 cut(s) | 82, 235 |
| SfcI | CTRYAG | 1 cut(s) | 249 |
| SinI | GGWCC | 2 cut(s) | 266, 714 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 126, 561 |
| SmlI | CTYRAG | 1 cut(s) | 936 |
| SmoI | CTYRAG | 1 cut(s) | 936 |
| Sse9I | AATT | 2 cut(s) | 65, 749 |
| SsiI | CCGC | 2 cut(s) | 4, 52 |
| SspI | AATATT | 1 cut(s) | 589 |
| SspMI | CTAG | 4 cut(s) | 263, 594, 800, 909 |
| StyI | CCWWGG | 1 cut(s) | 841 |
| TaaI | ACNGT | 2 cut(s) | 491, 949 |
| TaqI | TCGA | 3 cut(s) | 601, 747, 901 |
| TasI | AATT | 2 cut(s) | 65, 749 |
| TatI | WGTACW | 2 cut(s) | 944, 966 |
| TauI | GCSGC | 1 cut(s) | 55 |
| TfiI | GAWTC | 1 cut(s) | 744 |
| Tru1I | TTAA | 1 cut(s) | 755 |
| Tru9I | TTAA | 1 cut(s) | 755 |
| TscAI | CASTG | 3 cut(s) | 496, 568, 930 |
| TspDTI | ATGAA | 3 cut(s) | 144, 549, 864 |
| TspRI | CASTG | 3 cut(s) | 496, 568, 930 |
| VpaK11BI | GGWCC | 2 cut(s) | 266, 714 |
| XceI | RCATGY | 1 cut(s) | 125 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 650 |
| XspI | CTAG | 4 cut(s) | 263, 594, 800, 909 |