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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AloI | GAACNNNNNNTCC | 2 cut(s) | 59, 91 |
| AluBI | AGCT | 1 cut(s) | 30 |
| AluI | AGCT | 1 cut(s) | 30 |
| AseI | ATTAAT | 1 cut(s) | 44 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 59, 89 |
| BspHI | TCATGA | 1 cut(s) | 55 |
| BstDEI | CTNAG | 1 cut(s) | 31 |
| CciI | TCATGA | 1 cut(s) | 55 |
| CviAII | CATG | 1 cut(s) | 56 |
| CviJI | RGCY | 1 cut(s) | 30 |
| CviKI_1 | RGCY | 1 cut(s) | 30 |
| DdeI | CTNAG | 1 cut(s) | 31 |
| FaeI | CATG | 1 cut(s) | 59 |
| FaiI | YATR | 2 cut(s) | 39, 57 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 77, 109 |
| FatI | CATG | 1 cut(s) | 55 |
| FspEI | CC | 8 cut(s) | 8, 33, 50, 91, 93, 98, 99, 117 |
| Hin1II | CATG | 1 cut(s) | 59 |
| HinfI | GANTC | 1 cut(s) | 12 |
| Hpy166II | GTNNAC | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 2 cut(s) | 56, 114 |
| Hpy8I | GTNNAC | 1 cut(s) | 67 |
| HpyAV | CCTTC | 1 cut(s) | 125 |
| HpyCH4V | TGCA | 2 cut(s) | 90, 128 |
| HpyF3I | CTNAG | 1 cut(s) | 31 |
| Hsp92II | CATG | 1 cut(s) | 59 |
| MluCI | AATT | 2 cut(s) | 41, 45 |
| MseI | TTAA | 1 cut(s) | 44 |
| MslI | CAYNNNNRTG | 1 cut(s) | 60 |
| NlaIII | CATG | 1 cut(s) | 59 |
| PagI | TCATGA | 1 cut(s) | 55 |
| PfeI | GAWTC | 1 cut(s) | 12 |
| PshBI | ATTAAT | 1 cut(s) | 44 |
| RseI | CAYNNNNRTG | 1 cut(s) | 60 |
| SaqAI | TTAA | 1 cut(s) | 44 |
| SetI | ASST | 1 cut(s) | 32 |
| SgeI | CNNG | 5 cut(s) | 28, 68, 91, 106, 126 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 60 |
| Sse9I | AATT | 2 cut(s) | 41, 45 |
| TasI | AATT | 2 cut(s) | 41, 45 |
| TfiI | GAWTC | 1 cut(s) | 12 |
| Tru1I | TTAA | 1 cut(s) | 44 |
| Tru9I | TTAA | 1 cut(s) | 44 |
| VspI | ATTAAT | 1 cut(s) | 44 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 44 |