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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 123 |
| AgsI | TTSAA | 1 cut(s) | 101 |
| AluBI | AGCT | 1 cut(s) | 22 |
| AluI | AGCT | 1 cut(s) | 22 |
| BccI | CCATC | 1 cut(s) | 110 |
| BfaI | CTAG | 2 cut(s) | 23, 93 |
| BsaBI | GATNNNNATC | 1 cut(s) | 87 |
| Bse1I | ACTGG | 1 cut(s) | 5 |
| Bse8I | GATNNNNATC | 1 cut(s) | 87 |
| BseGI | GGATG | 2 cut(s) | 46, 88 |
| BseJI | GATNNNNATC | 1 cut(s) | 87 |
| BseNI | ACTGG | 1 cut(s) | 5 |
| BsrI | ACTGG | 1 cut(s) | 5 |
| BstF5I | GGATG | 2 cut(s) | 46, 88 |
| BtsCI | GGATG | 2 cut(s) | 46, 88 |
| BtsI | GCAGTG | 1 cut(s) | 118 |
| BtsIMutI | CAGTG | 1 cut(s) | 118 |
| Csp6I | GTAC | 1 cut(s) | 122 |
| CviJI | RGCY | 1 cut(s) | 22 |
| CviKI_1 | RGCY | 1 cut(s) | 22 |
| CviQI | GTAC | 1 cut(s) | 122 |
| FaiI | YATR | 2 cut(s) | 60, 108 |
| FokI | GGATG | 2 cut(s) | 53, 95 |
| FspBI | CTAG | 2 cut(s) | 23, 93 |
| FspEI | CC | 7 cut(s) | 18, 23, 41, 65, 102, 105, 146 |
| Hpy188I | TCNGA | 1 cut(s) | 82 |
| Hpy99I | CGWCG | 1 cut(s) | 35 |
| HpyCH4IV | ACGT | 1 cut(s) | 33 |
| HpyCH4V | TGCA | 1 cut(s) | 143 |
| HpySE526I | ACGT | 1 cut(s) | 33 |
| LpnPI | CCDG | 1 cut(s) | 18 |
| MaeI | CTAG | 2 cut(s) | 23, 93 |
| MaeII | ACGT | 1 cut(s) | 33 |
| MboII | GAAGA | 1 cut(s) | 56 |
| MluCI | AATT | 1 cut(s) | 76 |
| MnlI | CCTC | 2 cut(s) | 31, 38 |
| MslI | CAYNNNNRTG | 1 cut(s) | 111 |
| RsaI | GTAC | 1 cut(s) | 123 |
| RsaNI | GTAC | 1 cut(s) | 122 |
| RseI | CAYNNNNRTG | 1 cut(s) | 111 |
| SetI | ASST | 3 cut(s) | 24, 30, 36 |
| SgeI | CNNG | 4 cut(s) | 17, 35, 41, 105 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 111 |
| Sse9I | AATT | 1 cut(s) | 76 |
| SspI | AATATT | 1 cut(s) | 139 |
| SspMI | CTAG | 2 cut(s) | 23, 93 |
| TaiI | ACGT | 1 cut(s) | 36 |
| TaqI | TCGA | 1 cut(s) | 30 |
| TasI | AATT | 1 cut(s) | 76 |
| TatI | WGTACW | 1 cut(s) | 121 |
| TscAI | CASTG | 1 cut(s) | 118 |
| TspDTI | ATGAA | 3 cut(s) | 57, 84, 99 |
| TspRI | CASTG | 1 cut(s) | 118 |
| XspI | CTAG | 2 cut(s) | 23, 93 |