TNP1/EN/SPM transposase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 49 |
| AflII | CTTAAG | 1 cut(s) | 89 |
| AgsI | TTSAA | 1 cut(s) | 101 |
| AhlI | ACTAGT | 1 cut(s) | 140 |
| AoxI | GGCC | 2 cut(s) | 18, 54 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 75, 122 |
| AspS9I | GGNCC | 1 cut(s) | 18 |
| BcuI | ACTAGT | 1 cut(s) | 140 |
| BfaI | CTAG | 1 cut(s) | 141 |
| BfrI | CTTAAG | 1 cut(s) | 89 |
| BmgT120I | GGNCC | 1 cut(s) | 18 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 66, 96 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 49 |
| BseGI | GGATG | 2 cut(s) | 11, 126 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 49 |
| BshFI | GGCC | 2 cut(s) | 20, 56 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 49 |
| BsnI | GGCC | 2 cut(s) | 20, 56 |
| BspANI | GGCC | 2 cut(s) | 20, 56 |
| BspTI | CTTAAG | 1 cut(s) | 89 |
| BstAFI | CTTAAG | 1 cut(s) | 89 |
| BstF5I | GGATG | 2 cut(s) | 11, 126 |
| BsuRI | GGCC | 2 cut(s) | 20, 56 |
| BtsCI | GGATG | 2 cut(s) | 11, 126 |
| Cfr13I | GGNCC | 1 cut(s) | 18 |
| CviJI | RGCY | 3 cut(s) | 20, 42, 56 |
| CviKI_1 | RGCY | 3 cut(s) | 20, 42, 56 |
| EcoO109I | RGGNCCY | 1 cut(s) | 18 |
| FaiI | YATR | 2 cut(s) | 11, 13 |
| FokI | GGATG | 2 cut(s) | 18, 113 |
| FspBI | CTAG | 1 cut(s) | 141 |
| HaeIII | GGCC | 2 cut(s) | 20, 56 |
| HinfI | GANTC | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 1 cut(s) | 134 |
| LpnPI | CCDG | 1 cut(s) | 35 |
| MaeI | CTAG | 1 cut(s) | 141 |
| MlyI | GAGTC | 1 cut(s) | 75 |
| MmeI | TCCRAC | 1 cut(s) | 109 |
| MnlI | CCTC | 3 cut(s) | 9, 31, 46 |
| MroXI | GAANNNNTTC | 2 cut(s) | 75, 122 |
| MseI | TTAA | 2 cut(s) | 90, 153 |
| MslI | CAYNNNNRTG | 1 cut(s) | 154 |
| MspCI | CTTAAG | 1 cut(s) | 89 |
| PdmI | GAANNNNTTC | 2 cut(s) | 75, 122 |
| PleI | GAGTC | 1 cut(s) | 75 |
| PpsI | GAGTC | 1 cut(s) | 75 |
| PspPI | GGNCC | 1 cut(s) | 18 |
| RseI | CAYNNNNRTG | 1 cut(s) | 154 |
| SaqAI | TTAA | 2 cut(s) | 90, 153 |
| Sau96I | GGNCC | 1 cut(s) | 18 |
| SchI | GAGTC | 1 cut(s) | 75 |
| SetI | ASST | 2 cut(s) | 35, 125 |
| SgeI | CNNG | 4 cut(s) | 42, 62, 143, 153 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 154 |
| SmlI | CTYRAG | 1 cut(s) | 89 |
| SmoI | CTYRAG | 1 cut(s) | 89 |
| SpeI | ACTAGT | 1 cut(s) | 140 |
| SspMI | CTAG | 1 cut(s) | 141 |
| TaqI | TCGA | 1 cut(s) | 79 |
| Tru1I | TTAA | 2 cut(s) | 90, 153 |
| Tru9I | TTAA | 2 cut(s) | 90, 153 |
| TspDTI | ATGAA | 1 cut(s) | 115 |
| TspGWI | ACGGA | 1 cut(s) | 119 |
| Vha464I | CTTAAG | 1 cut(s) | 89 |
| XmnI | GAANNNNTTC | 2 cut(s) | 75, 122 |
| XspI | CTAG | 1 cut(s) | 141 |