Rorug01G0084700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000089
Physical Location & Seq
Reverse (-)
13763620 .. 13773432
9813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0084700.1

Sequence Viewer

Length: 3495 bp
ATGGAGCAAGGCATAGCAAAGCAAAATATGGCTGCTCTGACCAATGAAGCAGCTAGTTCTTCATCTTCTTCTCTCCCACTTTCTTCTACTGACCAAAATCATTATACATACCAGGTTTTCCTGAGTTTTCGAGGGGAGGATACACGCTTTAATTTCACAGATCATCTGCACACCGCTTTGGAGGACAGGGGAATTAAGACCTTCATAGATGATGAGCTCAGAAGAGGAGAGAAAATATCCGCGGCTCTTGTCAGAGCAATTGAGGAGTCCAGAGTTTCAATCATTGTCTTCTCTCAAAATTATGCTTCGTCAAGGTGGTGCTTAGATGAGCTGGTCAAGATACTTGAATGCAGAAAATCCAAAGGACAAGAAGTTAGACCTGTATTTTACAAGGTTGATCCAGCAGATGTACGACACCAAAGAGGTGCTTTCGGTGACGCATTTGCTACGCTTGATCAATGCAAATACAAAGACAGCATGGACAAATGGAAGGCAGCTCTTAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCGAGTCTGAGGCTAAATTTATCAGGAAGATTGTTGGGGAGTTGTCCGCCGGAGTAGTAAACCCTTCAAGTGAGTTGCAAGTAGCTGCATATCCAATTGGATTAGAGTCTTGTAGACAAGCTGTCAAAAGTCTTTTAAAAGCCGAGGAAAATATTGTTCGCATGGTAGGCATATGGGGGCCTGGTGGAATAGGAAAGACCACAATCGCGAAAGATGTGTTTAATTCAATTAGCTATAAGTTTGCATGTAGTTGTTTCTTGGCAGATGTTAGATCAAATGCCCTAGTCCAACTGCAAGAGACACTTTTATTTGATAGTTTAAGAGACTCAACTTTGAAGGTGAACAGTGTTGATAAAGGAGTCAGTTTGATAAAGACAAGGATGCAAAATAAAAAAGTTCTCTTAATCCTTGATGACGTGAGTCATTCTAGCCAATTACAGAACTTGGTTCCATCCCCTAATTGCTTTGGGCCGGGCAGTAGAATTCTCATAACAACAAGAGATAAACGTTGGCTAATTGCTCATGAAGTTGATGAAGTATACGAGGTAAAGATGTTAAATGATCATCACGCTTTGGAGTTGTTTAGCTTGCATGCATTCAAAAGAAATGGACCTCCAAGCGATTATCTGGAACTTGCACAACATGTTATAAGCTATGCCCAAGGCCTTCCATTAGCTTTGATACTCGTAGGCTCTCATCTATTTCGTAGACGCATAGAGGAGTGGAAAGCTACAATAGGTAGTTTTAAAGGAGGTCCTCAGGCAGAGATACAAGACGTTCTCAGACTAAGTTATGATGCTCTAGGAGCAGATTTAAAAGAACTATTTCTTGATATCGCTTGCTTCTTTAAAGGTAAGCATGTTGCCCGTGTGAAACCAATACTAGAAGCTCTCTACGACCTCAAAACAATGATGATTGGTATTGCACAACTCCAAGAAAAGGCCTTGATAAAAATTGACGAAGATGTGATTTGGATGCATGACTTGATAGAGGAAATGGGTAAAGATATAGTGTATCAAGAGTCACCTGGTGAGCCTGGAAAACGCAGTAGAGTGTGGAGTGAAGAAGATGTCAACCACATTCTAACAAATAATACAGGAACAAAGAAAGTTATAGGCATCCAGGTCTCGTGGCGATCATCTATGATATTTTTGAATGGTAAAAGCTTCTCACAGATGAAGAATCTTAGATATATTTCTATGAGCAAGGACCTGGAATTGTTTGCCTCAAAATCGTCTAAAATCAGGTCCAAACAGGAATATGAATCCTTTTCTGGAGACATTGATTATCTCTCCAACCAGTTGAGGTGGCTTGATTGGCCTGATAGTCCGTTGCAATCTTTTCCGTCCGATTTTCATGCAAACAAACTTGTAAGCCTCAATATTTCTGACAGCCACAGAATCACACGACTATGGGAGGGACGTAAGAATTTTTCAAAGCTAACACGTATGAATTTAAGGGGTTGTGAATCCCTAACAGGACTGCCATACTTCAGTGGAATACCCAACTTGAAAGAGTTGGATCTAGCAGATTGTGAAAATCTTACAACAATACCATGCAATGAGTTACAAAATCTGGAGATCCTAAATGTGAGTAGATGCTCAAATCTTGTTACATTTCAAACAAAAGCAAGTATTTCACATGATCATGACAGTGGCTCGCTAGCGCTTCCCAAGCTACTTGTACTGAAAATCAAAGGATGTAATTTATCATCTGCTGATTTCATTGGGAGTCTTGACTGCTTGAAAACATTAACCGAACTTGATCTCTCAAGTAACAATTTTGTTAGTGTTCCTGCACTCGGCAAATTTGTCAACTTGCCAAGGATCGACTTGAATTGTTGCAAGAGACTTAGAGAGATTCCAGAGCTTCCACCAAATATACTTGAGGTAAATGCGAGGGATTGCGAATCACTGGAGAGATTTTATATATGGCCCAAATCTTTGAAAATGATAGAGATGGACTTGTGTAATTGCCATAGATTTAGTTACAGTCTGGGATATGACATGGAGAAGATGGAAAATATTTTGCTGAATAATCAGAACAACTCCCCATTTACACTTGTATTACCCGGTAGTGAGGTTCCGAAGTGGTTCCATATTTCGAAGGAGGTTGCTGCTAATGAAAAAGACCTGGCATTGACATGTGCAGTTTCTTTTGAAATCCCAAGCAAATTGAATTGGGAGAACATAGGACTGGCTCTTTGTTCTGTTTTAGTTGAGGAAACTTTTAGTATTTTTATTGACGATCGAGCTGTTATTTCCATCAATGAGGAACTCATAGATCAGAGATCCAATCGGCACAGTCCAACGCCAATTGGCCATATGCGTCTGAATTATATTCCATTATCTGATGAAATAAAGGGAAAGGTGGATCAAAACTGTTGGTCGCATTATCACTGTCGAGTTCAATTTTACCCTGTTGATCAAATGAAAAGCTGTGGAGTTCACCTCGTATGCCAACCACCGAATGAATATTCAAATAAGATTGTAATGGCAGCTCCACCTGCCGATGAGAGTGGTATGCTAGACTGCCTTTCGTGGCTTTCATTGCCGTCAATTGAAGATGATTGTGAATCACTGGACACAGACGTTGGCCTAGACATAAGGCGTTGGCTTTCGAAAATCGAGGCACGGTTTCAAACATCGGGTAAACAAGCTCTTCAGGATTCTCTTAAGTCTAACATGGAAGCTTCAGTTGTACCTGCCTTTGAGAAGTCATGTAAAGCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAGGGAATGGTTGAACATGCAATTGTGGCTCTGCAGCATTTTGAGTCTGCACATTCGCCGCTAGCCCATGCTTTAAGGGAAGCTATTAGCTCTGCATCATCAGTGACTCAAACCCTAAGCGGACAACTGGCTGATGGTCAATGTAATCAGCATTCAGATCAGAATGGGGCAGTCTATCTGGACTGTTACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_23

Protein Analysis

1164

Amino Acids

130.84

Weight (kDa)

5.86

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 37 - 196 1.5e-54 TIR domain
TIR_2 PF13676 39 - 134 5.6e-17 TIR domain
NB-ARC PF00931 220 - 382 2.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 453 - 522 5.9e-13 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42700
fragaria_vesca FvH4_1g29233 FvH4_1g29373 FvH4_2g07142 FvH4_2g08144 FvH4_3g21852 FvH4_3g29221 FvH4_3g32731 FvH4_3g45091 FvH4_4g01311 FvH4_4g03220 FvH4_4g06631 FvH4_4g10040 FvH4_4g10363 FvH4_4g12371 FvH4_4g15323 FvH4_4g15580 FvH4_4g16173 FvH4_5g18843 FvH4_5g23172 FvH4_5g27660 FvH4_5g37213 FvH4_6g17382 FvH4_6g20172 FvH4_6g20173 FvH4_6g21082 FvH4_6g35541
malus_domestica MD02G1251500.v1.1 MD02G1252300.v1.1
pyrus_communis pycom01g02580 pycom01g08340 pycom01g08610 pycom11g01520 pycom12g04790
rosa_chinensis RchiOBHm_Chr0c16g0499821 RchiOBHm_Chr0c16g0499871 RchiOBHm_Chr0c28g0501051 RchiOBHm_Chr1g0313071 RchiOBHm_Chr1g0315701 RchiOBHm_Chr1g0318841 RchiOBHm_Chr1g0326261 RchiOBHm_Chr1g0357501 RchiOBHm_Chr2g0133581 RchiOBHm_Chr2g0134701 RchiOBHm_Chr2g0152521 RchiOBHm_Chr3g0455961 RchiOBHm_Chr3g0458231 RchiOBHm_Chr3g0460621 RchiOBHm_Chr3g0492791 RchiOBHm_Chr3g0492801 RchiOBHm_Chr3g0496631 RchiOBHm_Chr3g0496641 RchiOBHm_Chr4g0386541 RchiOBHm_Chr4g0415851 RchiOBHm_Chr4g0420561 RchiOBHm_Chr4g0422721 RchiOBHm_Chr4g0433611 RchiOBHm_Chr4g0444771 RchiOBHm_Chr5g0033771 RchiOBHm_Chr5g0059371 RchiOBHm_Chr5g0060161 RchiOBHm_Chr5g0064791 RchiOBHm_Chr5g0064801 RchiOBHm_Chr6g0269851 RchiOBHm_Chr6g0280321 RchiOBHm_Chr6g0287111 RchiOBHm_Chr7g0177401 RchiOBHm_Chr7g0200581 RchiOBHm_Chr7g0200591 RchiOBHm_Chr7g0231331
rosa_laevigata RLG00000001052 RLG00000001162 RLG00000002271 RLG00000002679 RLG00000007743 RLG00000009828 RLG00000009891 RLG00000009895 RLG00000009898 RLG00000017129 RLG00000018019 RLG00000018832 RLG00000018849 RLG00000019409 RLG00000019469 RLG00000019633 RLG00000019682 RLG00000020304 RLG00000024132 RLG00000027333 RLG00000028566 RLG00000031685 RLG00000031699 RLG00000034060 RLG00000034663 RLG00000035858 RLG00000036314
rosa_multiflora Rmu_sc0000178.1_g000001 Rmu_sc0000847.1_g000010 Rmu_sc0000861.1_g000033 Rmu_sc0001043.1_g000008 Rmu_sc0001499.1_g000050 Rmu_sc0001572.1_g000045 Rmu_sc0001768.1_g000043 Rmu_sc0001824.1_g000038 Rmu_sc0002037.1_g000022 Rmu_sc0002042.1_g000011 Rmu_sc0002322.1_g000001 Rmu_sc0002401.1_g000002 Rmu_sc0002438.1_g000018 Rmu_sc0002648.1_g000010 Rmu_sc0002784.1_g000047 Rmu_sc0003776.1_g000004 Rmu_sc0003780.1_g000005 Rmu_sc0004140.1_g000039 Rmu_sc0004596.1_g000033 Rmu_sc0004866.1_g000040 Rmu_sc0004964.1_g000011 Rmu_sc0005065.1_g000039 Rmu_sc0005137.1_g000032 Rmu_sc0005294.1_g000031 Rmu_sc0005426.1_g000005 Rmu_sc0005789.1_g000017 Rmu_sc0007891.1_g000008 Rmu_sc0008554.1_g000002 Rmu_sc0011363.1_g000002 Rmu_sc0020081.1_g000001 Rmu_ssc0000079.1_g000015
rosa_roxburghii Rroxscaffold_1G00010060 Rroxscaffold_1G00013840 Rroxscaffold_1G00031540 Rroxscaffold_1G00039640 Rroxscaffold_1G00048530 Rroxscaffold_2G00093720 Rroxscaffold_2G00093730 Rroxscaffold_2G00109830 Rroxscaffold_2G00109890 Rroxscaffold_2G00149920 Rroxscaffold_3G00274720 Rroxscaffold_4G00300310 Rroxscaffold_5G00347510 Rroxscaffold_5G00360920 Rroxscaffold_5G00362090 Rroxscaffold_5G00368170 Rroxscaffold_6G00391580 Rroxscaffold_7G00181720 Rroxscaffold_7G00209870
rosa_rugosa Rorug01G0071800 Rorug01G0071900 Rorug01G0072000 Rorug01G0072100 Rorug01G0084700 Rorug01G0104500 Rorug01G0104500 Rorug01G0134900.1 Rorug01G0135000.1 Rorug01G0183500 Rorug01G0256500 Rorug01G0312300 Rorug01G0312400 Rorug01G0351500 Rorug01G0384100 Rorug02G0039300 Rorug02G0319100 Rorug02G0319200 Rorug02G0319300 Rorug02G0461700 Rorug02G0531700 Rorug02G0617600 Rorug03G0155300 Rorug03G0190400 Rorug03G0248900 Rorug03G0249000 Rorug05G0141900 Rorug05G0325500 Rorug05G0325600 Rorug05G0369600 Rorug05G0490000 Rorug05G0490100.1 Rorug06G0009300 Rorug06G0035500 Rorug06G0082200 Rorug06G0133600 Rorug06G0133700 Rorug06G0149500 Rorug06G0149600 Rorug06G0149700 Rorug06G0351300 Rorug06G0443300 Rorug06G0443400 Rorug06G0443500 Rorug06G0443500 Rorug06G0443500 Rorug06G0480000 Rorug07G0260700 Rorug07G0260800 Rorug07G0289600 Rorug07G0289700 Rorug07G0319200 Rorug07G0319300
rosa_samantha Rh1AG098300 Rh1BG003900 Rh1BG262700 Rh1CG054000 Rh1CG054100 Rh1CG054200 Rh1CG232800 Rh1DG058300 Rh1DG058400 Rh1DG058500 Rh1DG085300 Rh1DG137700 Rh2AG234800 Rh2BG248300 Rh2CG045400 Rh2CG239300 Rh2CG239400 Rh2CG326600 Rh2CG349000 Rh2CG355400 Rh2CG402000 Rh2DG001000 Rh2DG242300 Rh3AG001600 Rh3AG306500 Rh3BG373600 Rh3CG001300 Rh3CG365600 Rh4AG013600 Rh4AG178000 Rh4AG198400 Rh4AG198600 Rh4AG416100 Rh4AG416300 Rh4BG029700 Rh4BG138600 Rh4BG150700 Rh4BG196400 Rh4BG427400 Rh4BG427600 Rh4CG442400 Rh4CG442500 Rh4DG171400 Rh4DG171500 Rh4DG171600 Rh4DG196200 Rh4DG423300 Rh4DG423400 Rh5AG490200 Rh5CG422300 Rh5CG428400 Rh5CG513400 Rh5CG519500 Rh5CG579300 Rh6AG225400 Rh6BG053200 Rh6BG195500 Rh6BG245600 Rh6DG161700 Rh6DG238400 Rh7AG348200 Rh7AG396700 Rh7AG396800 Rh7AG410600 Rh7BG445300 Rh7CG255900 Rh7CG365800 Rh7CG415100 Rh7CG415200 Rh7DG191000 Rh7DG191100 Rh7DG362700
rosa_wichuraiana Rw1G003060 Rw2G017950 Rw2G019900 Rw2G038280 Rw2G048340 Rw3G026970 Rw4G006460 Rw4G009350 Rw4G016820 Rw5G034510 Rw5G049530 Rw6G004160 Rw7G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
AarI CACCTGC 1 cut(s) 3055
Acc36I ACCTGC 2 cut(s) 3055, 3253
AccI GTMKAC 3 cut(s) 655, 1080, 1249
AccII CGCG 2 cut(s) 242, 749
AciI CCGC 6 cut(s) 174, 240, 242, 588, 3359, 3420
AclI AACGTT 1 cut(s) 1048
AclWI GGATC 6 cut(s) 392, 2070, 2116, 2375, 2826, 2922
AcoI YGGCCR 1 cut(s) 2860
AcsI RAATTY 5 cut(s) 557, 1023, 1969, 1993, 2348
AcuI CTGAAG 3 cut(s) 2017, 3188, 3219
AdeI CACNNNGTG 1 cut(s) 1571
AfaI GTAC 3 cut(s) 411, 2226, 3243
AfeI AGCGCT 1 cut(s) 2208
AfiI CCNNNNNNNGG 2 cut(s) 1480, 2342
AflII CTTAAG 2 cut(s) 500, 3215
AflIII ACRYGT 3 cut(s) 1183, 1985, 2684
AhdI GACNNNNNGTC 1 cut(s) 662
AjiI CACGTC 1 cut(s) 958
AjnI CCWGG 7 cut(s) 111, 721, 1567, 1576, 1662, 1752, 2673
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 5 cut(s) 833, 858, 1672, 1812, 2383
AlwI GGATC 6 cut(s) 392, 2070, 2116, 2375, 2826, 2922
Aor51HI AGCGCT 1 cut(s) 2208
AoxI GGCC 9 cut(s) 527, 719, 1010, 1204, 1482, 1859, 2474, 2860, 3136
ApeKI GCWGC 8 cut(s) 32, 50, 494, 509, 626, 2657, 3038, 3334
ApoI RAATTY 5 cut(s) 557, 1023, 1969, 1993, 2348
ArsI GACNNNNNNTTYG 2 cut(s) 2727, 2759
Asp700I GAANNNNTTC 2 cut(s) 1365, 2633
AspLEI GCGC 1 cut(s) 2209
AspS9I GGNCC 7 cut(s) 719, 1010, 1151, 1295, 1750, 1788, 2475
AsuC2I CCSGG 2 cut(s) 1014, 2613
AsuHPI GGTGA 5 cut(s) 446, 892, 1557, 1583, 2981
AsuII TTCGAA 2 cut(s) 2645, 3161
AsuNHI GCTAGC 2 cut(s) 2203, 3361
AvaII GGWCC 4 cut(s) 1151, 1295, 1750, 1788
AxyI CCTNAGG 1 cut(s) 1299
BalI TGGCCA 1 cut(s) 2862
BanII GRGCYC 1 cut(s) 219
BauI CACGAG 1 cut(s) 1669
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 8 cut(s) 19, 62, 506, 521, 613, 2644, 3050, 3346
BccI CCATC 7 cut(s) 519, 533, 1000, 2494, 2551, 2813, 3428
BceAI ACGGC 1 cut(s) 3079
BcgI CGANNNNNNTGC 2 cut(s) 1880, 1914
BciT130I CCWGG 7 cut(s) 113, 723, 1569, 1578, 1664, 1754, 2675
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 4 cut(s) 454, 1102, 2185, 2965
BcnI CCSGG 2 cut(s) 1014, 2613
BcoDI GTCTC 5 cut(s) 833, 858, 1672, 1812, 2383
BfmI CTRYAG 1 cut(s) 3332
BfoI RGCGCY 1 cut(s) 2210
BfrI CTTAAG 2 cut(s) 500, 3215
BfuAI ACCTGC 2 cut(s) 3055, 3253
BfuI GTATCC 1 cut(s) 133
Bme1390I CCNGG 9 cut(s) 113, 723, 1014, 1569, 1578, 1664, 1754, 2613, 2675
Bme18I GGWCC 4 cut(s) 1151, 1295, 1750, 1788
BmeRI GACNNNNNGTC 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 958
BmgT120I GGNCC 7 cut(s) 719, 1010, 1151, 1295, 1750, 1788, 2475
BmiI GGNNCC 4 cut(s) 720, 990, 2625, 2636
BmrFI CCNGG 9 cut(s) 113, 723, 1014, 1569, 1578, 1664, 1754, 2613, 2675
BmsI GCATC 7 cut(s) 912, 1327, 1506, 1668, 2129, 3277, 3404
BmtI GCTAGC 2 cut(s) 2207, 3365
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 280
BplI GAGNNNNNCTC 2 cut(s) 2976, 3008
BpmI CTGGAG 3 cut(s) 1836, 2138, 2477
Bpu10I CCTNAGC 1 cut(s) 3416
Bpu14I TTCGAA 2 cut(s) 2645, 3161
BpuEI CTTGAG 2 cut(s) 2296, 2447
BpuMI CCSGG 2 cut(s) 1014, 2613
BsaAI YACGTR 1 cut(s) 1988
BsaI GGTCTC 1 cut(s) 1672
BsaJI CCNNGG 4 cut(s) 240, 684, 1201, 2363
Bsc4I CCNNNNNNNGG 2 cut(s) 1480, 2342
Bse1I ACTGG 5 cut(s) 1840, 2460, 2742, 3126, 3432
Bse21I CCTNAGG 1 cut(s) 1299
Bse3DI GCAATG 2 cut(s) 2107, 3089
BseBI CCWGG 7 cut(s) 113, 723, 1569, 1578, 1664, 1754, 2675
BseDI CCNNGG 4 cut(s) 240, 684, 1201, 2363
BseGI GGATG 7 cut(s) 530, 544, 927, 992, 1521, 1659, 2246
BseLI CCNNNNNNNGG 2 cut(s) 1480, 2342
BseMI GCAATG 2 cut(s) 2107, 3089
BseMII CTCAG 5 cut(s) 113, 232, 540, 1313, 1336
BseNI ACTGG 5 cut(s) 1840, 2460, 2742, 3126, 3432
BseRI GAGGAG 3 cut(s) 240, 278, 1274
BseXI GCAGC 8 cut(s) 19, 62, 506, 521, 613, 2644, 3050, 3346
BsgI GTGCAG 4 cut(s) 152, 2322, 2709, 3333
Bsh1236I CGCG 2 cut(s) 242, 749
Bsh1285I CGRYCG 1 cut(s) 2791
BshFI GGCC 9 cut(s) 529, 721, 1012, 1206, 1484, 1861, 2476, 2862, 3138
BsiEI CGRYCG 1 cut(s) 2791
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 3 cut(s) 591, 1013, 2613
BslFI GGGAC 1 cut(s) 1974
BslI CCNNNNNNNGG 2 cut(s) 1480, 2342
BsmAI GTCTC 5 cut(s) 833, 858, 1672, 1812, 2383
BsmFI GGGAC 1 cut(s) 1974
BsmI GAATGC 3 cut(s) 353, 1136, 3451
BsnI GGCC 9 cut(s) 529, 721, 1012, 1206, 1484, 1861, 2476, 2862, 3138
Bso31I GGTCTC 1 cut(s) 1672
Bsp119I TTCGAA 2 cut(s) 2645, 3161
Bsp1286I GDGCHC 1 cut(s) 219
Bsp68I TCGCGA 1 cut(s) 749
BspACI CCGC 6 cut(s) 174, 240, 242, 588, 3359, 3420
BspANI GGCC 9 cut(s) 529, 721, 1012, 1206, 1484, 1861, 2476, 2862, 3138
BspCNI CTCAG 5 cut(s) 114, 231, 541, 1312, 1335
BspFNI CGCG 2 cut(s) 242, 749
BspHI TCATGA 2 cut(s) 1063, 2188
BspLI GGNNCC 4 cut(s) 720, 990, 2625, 2636
BspMAI CTGCAG 1 cut(s) 3336
BspMI ACCTGC 2 cut(s) 3055, 3253
BspOI GCTAGC 2 cut(s) 2207, 3365
BspPI GGATC 6 cut(s) 392, 2070, 2116, 2375, 2826, 2922
BspQI GCTCTTC 1 cut(s) 3207
BspT104I TTCGAA 2 cut(s) 2645, 3161
BspTI CTTAAG 2 cut(s) 500, 3215
BspTNI GGTCTC 1 cut(s) 1672
BsrDI GCAATG 2 cut(s) 2107, 3089
BsrI ACTGG 5 cut(s) 1840, 2460, 2742, 3126, 3432
BssECI CCNNGG 4 cut(s) 240, 684, 1201, 2363
BssNAI GTATAC 1 cut(s) 1081
BssSI CACGAG 1 cut(s) 1669
BssT1I CCWWGG 2 cut(s) 1201, 2363
Bst1107I GTATAC 1 cut(s) 1081
Bst2BI CACGAG 1 cut(s) 1669
Bst2UI CCWGG 7 cut(s) 113, 723, 1569, 1578, 1664, 1754, 2675
Bst4CI ACNGT 8 cut(s) 887, 2195, 2534, 2846, 2924, 2942, 3177, 3485
Bst6I CTCTTC 2 cut(s) 217, 3207
BstAFI CTTAAG 2 cut(s) 500, 3215
BstBAI YACGTR 1 cut(s) 1988
BstBI TTCGAA 2 cut(s) 2645, 3161
BstC8I GCNNGC 6 cut(s) 1130, 1134, 1381, 2201, 2205, 3363
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 7 cut(s) 530, 544, 927, 992, 1521, 1659, 2246
BstFNI CGCG 2 cut(s) 242, 749
BstH2I RGCGCY 1 cut(s) 2210
BstHHI GCGC 1 cut(s) 2209
BstMAI GTCTC 5 cut(s) 833, 858, 1672, 1812, 2383
BstMCI CGRYCG 1 cut(s) 2791
BstMWI GCNNNNNNNGC 7 cut(s) 708, 1346, 1858, 2215, 3047, 3091, 3326
BstNI CCWGG 7 cut(s) 113, 723, 1569, 1578, 1664, 1754, 2675
BstNSI RCATGY 6 cut(s) 789, 1136, 1187, 1403, 2688, 3320
BstPAI GACNNNNGTC 1 cut(s) 960
BstSCI CCNGG 9 cut(s) 111, 721, 1012, 1567, 1576, 1662, 1752, 2611, 2673
BstSFI CTRYAG 1 cut(s) 3332
BstUI CGCG 2 cut(s) 242, 749
BstV1I GCAGC 8 cut(s) 19, 62, 506, 521, 613, 2644, 3050, 3346
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 3 cut(s) 2062, 2121, 2831
BstYI RGATCY 3 cut(s) 2062, 2121, 2831
BstZ17I GTATAC 1 cut(s) 1081
Bsu36I CCTNAGG 1 cut(s) 1299
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 9 cut(s) 529, 721, 1012, 1206, 1484, 1861, 2476, 2862, 3138
BtgI CCRYGG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 958
BtsCI GGATG 7 cut(s) 530, 544, 927, 992, 1521, 1659, 2246
BtsIMutI CAGTG 7 cut(s) 892, 2041, 2200, 2453, 2938, 3119, 3408
BtuMI TCGCGA 1 cut(s) 749
BveI ACCTGC 2 cut(s) 3055, 3253
Cac8I GCNNGC 6 cut(s) 1130, 1134, 1381, 2201, 2205, 3363
CciI TCATGA 2 cut(s) 1063, 2188
CfoI GCGC 1 cut(s) 2209
Cfr13I GGNCC 7 cut(s) 719, 1010, 1151, 1295, 1750, 1788, 2475
Cfr42I CCGCGG 1 cut(s) 243
CseI GACGC 3 cut(s) 446, 1260, 2858
CsiI ACCWGGT 2 cut(s) 111, 1567
Csp6I GTAC 3 cut(s) 410, 2225, 3242
CviQI GTAC 3 cut(s) 410, 2225, 3242
DraI TTTAAA 4 cut(s) 678, 1288, 1356, 1390
DraIII CACNNNGTG 1 cut(s) 1571
DriI GACNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 2860
Eam1104I CTCTTC 2 cut(s) 217, 3207
Eam1105I GACNNNNNGTC 1 cut(s) 662
EarI CTCTTC 2 cut(s) 217, 3207
EciI GGCGGA 1 cut(s) 577
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 1201, 2363
Eco147I AGGCCT 2 cut(s) 1206, 1484
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 1672
Eco32I GATATC 1 cut(s) 1375
Eco47I GGWCC 4 cut(s) 1151, 1295, 1750, 1788
Eco47III AGCGCT 1 cut(s) 2208
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 2017, 3188, 3219
Eco81I CCTNAGG 1 cut(s) 1299
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 3 cut(s) 719, 1295, 1750
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 7 cut(s) 111, 721, 1567, 1576, 1662, 1752, 2673
EcoRV GATATC 1 cut(s) 1375
EcoT14I CCWWGG 2 cut(s) 1201, 2363
EcoT22I ATGCAT 2 cut(s) 1138, 1521
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 2 cut(s) 1201, 2363
FalI AAGNNNNNCTT 4 cut(s) 412, 444, 828, 860
FaqI GGGAC 1 cut(s) 1974
FauNDI CATATG 2 cut(s) 713, 2865
FbaI TGATCA 4 cut(s) 454, 1102, 2185, 2965
FblI GTMKAC 3 cut(s) 655, 1080, 1249
FokI GGATG 7 cut(s) 537, 551, 934, 979, 1528, 1646, 2253
FriOI GRGCYC 1 cut(s) 219
GlaI GCGC 1 cut(s) 2208
GsuI CTGGAG 3 cut(s) 1836, 2138, 2477
HaeII RGCGCY 1 cut(s) 2210
HaeIII GGCC 9 cut(s) 529, 721, 1012, 1206, 1484, 1861, 2476, 2862, 3138
HapII CCGG 3 cut(s) 591, 1013, 2613
HgaI GACGC 3 cut(s) 446, 1260, 2858
HhaI GCGC 1 cut(s) 2209
Hin6I GCGC 1 cut(s) 2207
HinP1I GCGC 1 cut(s) 2207
HincII GTYRAC 2 cut(s) 1615, 2356
HindII GTYRAC 2 cut(s) 1615, 2356
HindIII AAGCTT 2 cut(s) 1705, 3231
HpaII CCGG 3 cut(s) 591, 1013, 2613
HphI GGTGA 5 cut(s) 446, 892, 1557, 1583, 2981
Hpy166II GTNNAC 9 cut(s) 601, 656, 883, 1081, 1250, 1615, 2356, 2989, 3194
Hpy8I GTNNAC 9 cut(s) 601, 656, 883, 1081, 1250, 1615, 2356, 2989, 3194
HpyAV CCTTC 7 cut(s) 211, 484, 615, 871, 1217, 2641, 3295
HpyCH4III ACNGT 8 cut(s) 887, 2195, 2534, 2846, 2924, 2942, 3177, 3485
HpyCH4IV ACGT 6 cut(s) 957, 1048, 1317, 1963, 1987, 3132
HpyF10VI GCNNNNNNNGC 7 cut(s) 708, 1346, 1858, 2215, 3047, 3091, 3326
HpySE526I ACGT 6 cut(s) 957, 1048, 1317, 1963, 1987, 3132
HspAI GCGC 1 cut(s) 2207
Ksp22I TGATCA 4 cut(s) 454, 1102, 2185, 2965
KspI CCGCGG 1 cut(s) 243
LguI GCTCTTC 1 cut(s) 3207
LmnI GCTCC 3 cut(s) 4, 1346, 3046
Lsp1109I GCAGC 8 cut(s) 19, 62, 506, 521, 613, 2644, 3050, 3346
LweI GCATC 7 cut(s) 912, 1327, 1506, 1668, 2129, 3277, 3404
MabI ACCWGGT 2 cut(s) 111, 1567
MaeII ACGT 6 cut(s) 957, 1048, 1317, 1963, 1987, 3132
MaeIII GTNAC 8 cut(s) 434, 1563, 2106, 2152, 2315, 2528, 3403, 3485
MfeI CAATTG 5 cut(s) 258, 636, 2856, 3099, 3321
MflI RGATCY 3 cut(s) 2062, 2121, 2831
MhlI GDGCHC 1 cut(s) 219
MlsI TGGCCA 1 cut(s) 2862
MluNI TGGCCA 1 cut(s) 2862
MmeI TCCRAC 4 cut(s) 853, 1860, 2040, 2873
Mox20I TGGCCA 1 cut(s) 2862
Mph1103I ATGCAT 2 cut(s) 1138, 1521
MroXI GAANNNNTTC 2 cut(s) 1365, 2633
MscI TGGCCA 1 cut(s) 2862
MslI CAYNNNNRTG 4 cut(s) 1951, 2187, 2193, 2683
Msp20I TGGCCA 1 cut(s) 2862
MspA1I CMGCKG 1 cut(s) 242
MspCI CTTAAG 2 cut(s) 500, 3215
MspI CCGG 3 cut(s) 591, 1013, 2613
MspR9I CCNGG 9 cut(s) 113, 723, 1014, 1569, 1578, 1664, 1754, 2613, 2675
MunI CAATTG 5 cut(s) 258, 636, 2856, 3099, 3321
Mva1269I GAATGC 3 cut(s) 353, 1136, 3451
MvaI CCWGG 7 cut(s) 113, 723, 1569, 1578, 1664, 1754, 2675
MvnI CGCG 2 cut(s) 242, 749
MwoI GCNNNNNNNGC 7 cut(s) 708, 1346, 1858, 2215, 3047, 3091, 3326
NciI CCSGG 2 cut(s) 1014, 2613
NdeI CATATG 2 cut(s) 713, 2865
NheI GCTAGC 2 cut(s) 2203, 3361
NlaIV GGNNCC 4 cut(s) 720, 990, 2625, 2636
NmeAIII GCCGAG 2 cut(s) 709, 2322
NmuCI GTSAC 3 cut(s) 434, 1563, 3403
NruI TCGCGA 1 cut(s) 749
NsiI ATGCAT 2 cut(s) 1138, 1521
NspI RCATGY 6 cut(s) 789, 1136, 1187, 1403, 2688, 3320
NspV TTCGAA 2 cut(s) 2645, 3161
PaeI GCATGC 1 cut(s) 1136
PagI TCATGA 2 cut(s) 1063, 2188
PaqCI CACCTGC 1 cut(s) 3055
PceI AGGCCT 2 cut(s) 1206, 1484
PciI ACATGT 2 cut(s) 1183, 2684
PciSI GCTCTTC 1 cut(s) 3207
PctI GAATGC 3 cut(s) 353, 1136, 3451
PdmI GAANNNNTTC 2 cut(s) 1365, 2633
PfeI GAWTC 8 cut(s) 1723, 1805, 1941, 2009, 2401, 2450, 3116, 3209
Ple19I CGATCG 1 cut(s) 2791
Ppu21I YACGTR 1 cut(s) 1988
PpuMI RGGWCCY 2 cut(s) 1295, 1750
PscI ACATGT 2 cut(s) 1183, 2684
PshAI GACNNNNGTC 1 cut(s) 960
PsiI TTATAA 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 1048
Psp5II RGGWCCY 2 cut(s) 1295, 1750
Psp6I CCWGG 7 cut(s) 111, 721, 1567, 1576, 1662, 1752, 2673
PspGI CCWGG 7 cut(s) 111, 721, 1567, 1576, 1662, 1752, 2673
PspN4I GGNNCC 4 cut(s) 720, 990, 2625, 2636
PspPI GGNCC 7 cut(s) 719, 1010, 1151, 1295, 1750, 1788, 2475
PspPPI RGGWCCY 2 cut(s) 1295, 1750
PstI CTGCAG 1 cut(s) 3336
PsuI RGATCY 3 cut(s) 2062, 2121, 2831
PvuI CGATCG 1 cut(s) 2791
RruI TCGCGA 1 cut(s) 749
RsaI GTAC 3 cut(s) 411, 2226, 3243
RsaNI GTAC 3 cut(s) 410, 2225, 3242
RseI CAYNNNNRTG 4 cut(s) 1951, 2187, 2193, 2683
SacI GAGCTC 1 cut(s) 219
SacII CCGCGG 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 3207
Sau96I GGNCC 7 cut(s) 719, 1010, 1151, 1295, 1750, 1788, 2475
ScrFI CCNGG 9 cut(s) 113, 723, 1014, 1569, 1578, 1664, 1754, 2613, 2675
SduI GDGCHC 1 cut(s) 219
SexAI ACCWGGT 2 cut(s) 111, 1567
SfaNI GCATC 7 cut(s) 912, 1327, 1506, 1668, 2129, 3277, 3404
SfcI CTRYAG 1 cut(s) 3332
Sfr303I CCGCGG 1 cut(s) 243
SfuI TTCGAA 2 cut(s) 2645, 3161
SgrBI CCGCGG 1 cut(s) 243
SinI GGWCC 4 cut(s) 1151, 1295, 1750, 1788
SmiMI CAYNNNNRTG 4 cut(s) 1951, 2187, 2193, 2683
SmlI CTYRAG 4 cut(s) 500, 2311, 2426, 3215
SmoI CTYRAG 4 cut(s) 500, 2311, 2426, 3215
SphI GCATGC 1 cut(s) 1136
SseBI AGGCCT 2 cut(s) 1206, 1484
SsiI CCGC 6 cut(s) 174, 240, 242, 588, 3359, 3420
SspI AATATT 4 cut(s) 694, 1924, 2566, 3017
SstI GAGCTC 1 cut(s) 219
StuI AGGCCT 2 cut(s) 1206, 1484
StyD4I CCNGG 9 cut(s) 111, 721, 1012, 1567, 1576, 1662, 1752, 2611, 2673
StyI CCWWGG 2 cut(s) 1201, 2363
TaaI ACNGT 8 cut(s) 887, 2195, 2534, 2846, 2924, 2942, 3177, 3485
TaiI ACGT 6 cut(s) 960, 1051, 1320, 1966, 1990, 3135
TaqI TCGA 7 cut(s) 130, 2370, 2645, 2791, 2944, 3161, 3168
TatI WGTACW 1 cut(s) 2224
TauI GCSGC 2 cut(s) 245, 3361
TfiI GAWTC 8 cut(s) 1723, 1805, 1941, 2009, 2401, 2450, 3116, 3209
TscAI CASTG 7 cut(s) 892, 2041, 2200, 2460, 2945, 3126, 3408
TseFI GTSAC 3 cut(s) 434, 1563, 3403
TseI GCWGC 8 cut(s) 32, 50, 494, 509, 626, 2657, 3038, 3334
Tsp45I GTSAC 3 cut(s) 434, 1563, 3403
TspGWI ACGGA 2 cut(s) 1860, 1875
TspRI CASTG 7 cut(s) 892, 2041, 2200, 2460, 2945, 3126, 3408
Vha464I CTTAAG 2 cut(s) 500, 3215
VpaK11BI GGWCC 4 cut(s) 1151, 1295, 1750, 1788
XapI RAATTY 5 cut(s) 557, 1023, 1969, 1993, 2348
XceI RCATGY 6 cut(s) 789, 1136, 1187, 1403, 2688, 3320
XmiI GTMKAC 3 cut(s) 655, 1080, 1249
XmnI GAANNNNTTC 2 cut(s) 1365, 2633
Zsp2I ATGCAT 2 cut(s) 1138, 1521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.