AT5G22720

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000062
Physical Location & Seq
Reverse (-)
7549565 .. 7551174
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22720.1

Sequence Viewer

Length: 1407 bp
ATGGAGGAAACGAAAGTGAAACGAACATGCCTCGAACGTACTGTATGTTCTTCAAAAGCTAAAGAGGATTTGATAAGCCAGTTACCTGACTCTTTGATAACTCAGATACTCTTTTATCTTCAGACAAAGAAAGCTGTTACGACTAGTGTTTTGTCCAAGAGATGGAGAAGTCTATGGCTTTCGACTCCCGGGTTGGTTTTGATCTCTAATGACTTTACAGATTACAATGCTTTTGTGAGCTTTGTGGACAAGTTCCTAGGTTTCTCTAGGGAACAGAAGTTATGCTTGCACAAGCTCAAGCTAAGCATTCGTAAGGGTGAGAATGATCAGGATTGTGTCACACGTTGGATAGATTTTGTGGCTACGCCTAAACTTAAGCATCTTGATGTTGAGATTGGTCCTACGAGGTGTGAGTGTTTTGAAGTGATACCCTTAAGCCTTTATAGCTGTGAGTCACTCCTTTACTTAAGACTAAATCATGTATGCTTGGGTAAGTTTGAGTCTGTTTCTTTACCATGTCTCAAGACAATGAGCTTAGAACAAAACATATATGCTAACGAGGCGGATCTAGAGTCTCTTATCTCAACTTGTCCTGTTCTTGAAGATTTGAGCTTTGTGAGTGGGGCATATGATAAAGTAAACGTTTTACGAGTGCAATCTCAGACATTAACCAGTCTCAATATAGAAGGTTGTGTTGAGTATTTGGATCTTGACAAGTCTGAGGTTTTGATTGATGCCACTAGACTCAAGTATTTGAATCTTGAAGCTGATCAATATGAGAGTAAAACCATAAGAAACTCTGGCTCTTTAACCAAGGTCAATCTTCTTGGCTATTTTCATATGAAGAATAATGATGATGATGATGAAGTCGACTTACAAAAGCGAGATATGGTACATAATTTTTTCACCAGTATATCGGGAGTCAGCGATATGAAAATCTCTTCCCAAGCGTTCGCGTTGTTCATCATGAATACCATGCCATTTTCACCCAAATTTTGCAACTTGTCCTGTTTGGAAGTAGAAATTTTCTTACCCTCACTGGAAACGCTGCCAACGTTTCTTGAAAGCTTTCCAAATCTCAAGTCCCTAATCTTGGGGTTACGTTATTGGACTCCGAAAAAAGAATTAAGACTCTCGTTTGTGCCTCGGTGTTTACTGTCATCGCTCGAGTTTGTAGAGATAAAAGGATGCAGTAGAAGTAACATGGAACGAGTAAAGTACGTTGGAGAACCTATTGAAACGAAACTAGCAAGATACTTTGTAGAGAACTCAACAATCCTCAAAAAACTTGTTCTGCCTTTGAGATCTTCCACGCCAGAAGAAATATATTCCGTCGACTTCTGGAATTTCCTTGAATTTCCTAGAAGATCTAGCATTTGTCAAATCACATATGTCGCTGGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000166 GO:0000186 GO:0000226 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010225 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010638 GO:0010646 GO:0010647 GO:0010941 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0030554 GO:0030587 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032153 GO:0032155 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032270 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032872 GO:0032874 GO:0033043 GO:0033044 GO:0033554 GO:0033674 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045935 GO:0045937 GO:0046328 GO:0046330 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070302 GO:0070304 GO:0071554 GO:0071704 GO:0071840 GO:0071852 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0080135 GO:0090702 GO:0097159 GO:0097367 GO:0097435 GO:0099120 GO:0099568 GO:0140096 GO:1900744 GO:1900745 GO:1901265 GO:1901363 GO:1901564 GO:1902531 GO:1902533 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

468

Amino Acids

53.63

Weight (kDa)

5.93

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 25 - 61 5.5e-07 F-box domain
LRR_At5g56370 PF24758 114 - 284 7.8e-16 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 173 - 198 3.4e-07 Leucine Rich Repeat
FBD PF08387 379 - 432 8.7e-13 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13780 AT1G13780 AT1G16930 AT1G16930 AT1G16930 AT1G16940 AT1G16945 AT1G51370 AT1G51370 AT1G51370 AT1G60400 AT1G60410 AT1G66290 AT1G66300 AT1G66300 AT1G66310 AT1G66320 AT1G66640 AT1G69630 AT1G78730 AT1G78740 AT1G78750 AT1G78750 AT1G78760 AT1G78840 AT1G78840 AT1G80470 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G27505 AT3G19085 AT3G19085 AT3G23955 AT3G23955 AT4G13985 AT5G18770 AT5G18770 AT5G18780 AT5G18780 AT5G18780 AT5G18780 AT5G22590 AT5G22600 AT5G22600 AT5G22610 AT5G22610 AT5G22660 AT5G22660 AT5G22670 AT5G22680 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22720 AT5G22730 AT5G22730 AT5G22730 AT5G25850 AT5G27750 AT5G27750 AT5G44490 AT5G44490 AT5G44940 AT5G44950 AT5G44960 AT5G44970 AT5G44980 AT5G44980 AT5G44980 AT5G44980 AT5G53592 AT5G53635 AT5G53637 AT5G53640 AT5G53840
fragaria_vesca FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g01470 FvH4_1g01500 FvH4_1g06320 FvH4_1g06320 FvH4_1g09701 FvH4_1g09890 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_3g17060 FvH4_3g17061 FvH4_3g17061 FvH4_3g17061 FvH4_3g17080 FvH4_3g17080 FvH4_3g17080 FvH4_3g17090 FvH4_3g17100 FvH4_3g17110 FvH4_3g17130 FvH4_3g17160 FvH4_3g17160 FvH4_3g17161 FvH4_3g17190 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g21162 FvH4_3g21162 FvH4_3g21190 FvH4_4g14821 FvH4_4g14841 FvH4_4g14850 FvH4_5g35600 FvH4_6g23651 FvH4_6g38031
malus_domestica MD03G1246000.v1.1 MD03G1252900.v1.1 MD03G1253200.v1.1 MD03G1253500.v1.1 MD03G1253700.v1.1 MD08G1193400.v1.1 MD11G1256200.v1.1 MD11G1265900.v1.1 MD11G1266000.v1.1 MD11G1266700.v1.1 MD11G1266800.v1.1 MD11G1266900.v1.1 MD11G1267000.v1.1 MD11G1274900.v1.1 MD11G1275300.v1.1
prunus_persica Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158800_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G159000_v2.0.a1
pyrus_communis pycom03g19500 pycom03g20040 pycom03g20080 pycom03g20130 pycom03g20140 pycom08g16650 pycom11g22660 pycom11g23380 pycom11g23390 pycom11g23450 pycom11g23470 pycom11g23480 pycom11g23490 pycom11g24250
rosa_chinensis RchiOBHm_Chr1g0321381 RchiOBHm_Chr4g0417361 RchiOBHm_Chr4g0417391 RchiOBHm_Chr4g0417401 RchiOBHm_Chr4g0417411 RchiOBHm_Chr4g0417471 RchiOBHm_Chr5g0028391 RchiOBHm_Chr5g0028401 RchiOBHm_Chr5g0028441 RchiOBHm_Chr5g0028451 RchiOBHm_Chr5g0028461 RchiOBHm_Chr5g0028471 RchiOBHm_Chr5g0028481 RchiOBHm_Chr5g0028491 RchiOBHm_Chr5g0028501 RchiOBHm_Chr5g0028511 RchiOBHm_Chr5g0028521 RchiOBHm_Chr5g0028541 RchiOBHm_Chr5g0028551 RchiOBHm_Chr5g0028581 RchiOBHm_Chr5g0040081 RchiOBHm_Chr7g0192741 RchiOBHm_Chr7g0237071
rosa_laevigata RLG00000007914 RLG00000033090 RLG00000033092 RLG00000033096 RLG00000033097 RLG00000033098 RLG00000033099 RLG00000033100
rosa_multiflora Rmu_sc0000103.1_g000060 Rmu_sc0000272.1_g000005 Rmu_sc0000272.1_g000006 Rmu_sc0001134.1_g000002 Rmu_sc0001561.1_g000020 Rmu_sc0001561.1_g000021 Rmu_sc0001575.1_g000002 Rmu_sc0001575.1_g000007 Rmu_sc0001575.1_g000008 Rmu_sc0001575.1_g000010 Rmu_sc0001575.1_g000013 Rmu_sc0002131.1_g000038 Rmu_sc0004142.1_g000020 Rmu_sc0004142.1_g000021 Rmu_sc0004142.1_g000026 Rmu_sc0004142.1_g000027 Rmu_sc0004142.1_g000028 Rmu_sc0005594.1_g000010 Rmu_sc0006771.1_g000001 Rmu_sc0006771.1_g000002 Rmu_sc0006771.1_g000003 Rmu_sc0006771.1_g000004 Rmu_sc0006771.1_g000007 Rmu_sc0008526.1_g000014 Rmu_sc0009797.1_g000001
rosa_roxburghii Rroxscaffold_1G00041210 Rroxscaffold_1G00051210 Rroxscaffold_1G00051220 Rroxscaffold_1G00051230 Rroxscaffold_1G00051240 Rroxscaffold_1G00051280 Rroxscaffold_1G00051310 Rroxscaffold_3G00224520 Rroxscaffold_3G00224530 Rroxscaffold_5G00361120 Rroxscaffold_6G00412010
rosa_rugosa Rorug04G0147300 Rorug04G0147400 Rorug04G0147500 Rorug04G0147700 Rorug04G0147800 Rorug05G0107100 Rorug05G0107300 Rorug05G0107600 Rorug05G0107600 Rorug05G0107700 Rorug05G0107800 Rorug05G0107900 Rorug05G0108000 Rorug05G0108100 Rorug05G0108200 Rorug05G0108400 Rorug05G0108500 Rorug05G0108600 Rorug05G0108700 Rorug05G0108900 Rorug05G0109000 Rorug05G0109100 Rorug05G0109300 Rorug05G0109400 Rorug05G0109500 Rorug05G0183200 Rorug05G0183300 Rorug05G0183400 Rorug07G0187200 Rorug07G0301700 Rorug07G0301700 Rorug07G0301800
rosa_samantha Rh1AG413100 Rh1CG047800 Rh4AG210900 Rh4AG211000 Rh4AG211100 Rh4BG141000 Rh4BG211300 Rh4CG221700 Rh4CG221800 Rh4CG221900 Rh4DG208700 Rh4DG208800 Rh4DG208900 Rh5AG199800 Rh5AG200100 Rh5AG200200 Rh5AG200500 Rh5AG200600 Rh5AG200700 Rh5AG200800 Rh5AG200900 Rh5AG201000 Rh5AG201100 Rh5AG201300 Rh5AG201400 Rh5AG269900 Rh5BG198300 Rh5BG198500 Rh5BG198900 Rh5BG199000 Rh5BG199400 Rh5BG199500 Rh5BG199600 Rh5BG199700 Rh5BG199800 Rh5BG273400 Rh5CG218400 Rh5CG219200 Rh5CG219300 Rh5CG219400 Rh5CG219500 Rh5CG219700 Rh5CG219800 Rh5CG306300 Rh5CG306400 Rh5DG200300 Rh5DG200500 Rh5DG200600 Rh5DG201100 Rh5DG201200 Rh5DG201300 Rh5DG201400 Rh5DG201500 Rh5DG201600 Rh5DG201700 Rh5DG201800 Rh5DG201900 Rh5DG202100 Rh5DG202400 Rh5DG202500 Rh5DG203100 Rh5DG281500 Rh7AG457900 Rh7BG320900 Rh7BG428600 Rh7CG476000 Rh7DG445100 Rh7DG445200
rosa_wichuraiana Rw0G014200 Rw0G014210 Rw1G003950 Rw4G017810 Rw4G017820 Rw4G017850 Rw5G018220 Rw5G018230 Rw5G018250 Rw5G018260 Rw5G018270 Rw5G018280 Rw5G025230 Rw7G037950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 162
AccI GTMKAC 2 cut(s) 870, 1335
AccII CGCG 1 cut(s) 956
AciI CCGC 1 cut(s) 563
AclI AACGTT 2 cut(s) 642, 1055
AclWI GGATC 2 cut(s) 573, 714
AcsI RAATTY 4 cut(s) 992, 1023, 1345, 1355
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 3 cut(s) 40, 894, 1220
AfiI CCNNNNNNNGG 2 cut(s) 162, 1093
AflII CTTAAG 3 cut(s) 374, 433, 466
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 8 cut(s) 54, 422, 602, 757, 764, 1064, 1238, 1355
AhlI ACTAGT 1 cut(s) 143
Alw26I GTCTC 3 cut(s) 524, 579, 680
AlwI GGATC 2 cut(s) 573, 714
Ama87I CYCGRG 2 cut(s) 188, 1166
AoxI GGCC 1 cut(s) 1399
ApeKI GCWGC 1 cut(s) 1048
ApoI RAATTY 4 cut(s) 992, 1023, 1345, 1355
ArsI GACNNNNNNTTYG 4 cut(s) 133, 165, 1067, 1099
Asp700I GAANNNNTTC 1 cut(s) 1068
AspA2I CCTAGG 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 398
AsuC2I CCSGG 2 cut(s) 189, 190
AsuHPI GGTGA 3 cut(s) 329, 898, 978
AvaI CYCGRG 2 cut(s) 188, 1166
AvaII GGWCC 1 cut(s) 398
AvrII CCTAGG 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 858, 890
BbvI GCAGC 1 cut(s) 1035
BccI CCATC 1 cut(s) 156
BclI TGATCA 2 cut(s) 325, 769
BcnI CCSGG 2 cut(s) 189, 190
BcoDI GTCTC 3 cut(s) 524, 579, 680
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 8 cut(s) 144, 257, 267, 569, 741, 1247, 1362, 1371
BfrI CTTAAG 3 cut(s) 374, 433, 466
BglII AGATCT 2 cut(s) 1304, 1367
BisI GCNGC 1 cut(s) 1049
BlnI CCTAGG 1 cut(s) 256
BlpI GCTNAGC 1 cut(s) 302
BlsI GCNGC 1 cut(s) 1050
Bme1390I CCNGG 2 cut(s) 189, 190
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 2 cut(s) 188, 1166
BmgT120I GGNCC 1 cut(s) 398
BmrFI CCNGG 2 cut(s) 189, 190
BmsI GCATC 3 cut(s) 388, 724, 1178
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 4 cut(s) 281, 506, 731, 1064
BpuMI CCSGG 2 cut(s) 189, 190
BsaJI CCNNGG 4 cut(s) 188, 256, 813, 1145
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 1093
Bse1I ACTGG 4 cut(s) 79, 672, 909, 1044
BseDI CCNNGG 4 cut(s) 188, 256, 813, 1145
BseGI GGATG 1 cut(s) 1193
BseLI CCNNNNNNNGG 2 cut(s) 162, 1093
BseMII CTCAG 3 cut(s) 116, 674, 711
BseNI ACTGG 4 cut(s) 79, 672, 909, 1044
BseXI GCAGC 1 cut(s) 1035
Bsh1236I CGCG 1 cut(s) 956
BshFI GGCC 1 cut(s) 1401
BsiHKCI CYCGRG 2 cut(s) 188, 1166
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 1069
BslI CCNNNNNNNGG 2 cut(s) 162, 1093
BsmAI GTCTC 3 cut(s) 524, 579, 680
BsmFI GGGAC 1 cut(s) 1069
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 1 cut(s) 1401
BsoBI CYCGRG 2 cut(s) 188, 1166
Bsp143I GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
Bsp1720I GCTNAGC 1 cut(s) 302
BspACI CCGC 1 cut(s) 563
BspANI GGCC 1 cut(s) 1401
BspCNI CTCAG 3 cut(s) 115, 673, 712
BspFNI CGCG 1 cut(s) 956
BspHI TCATGA 1 cut(s) 966
BspPI GGATC 2 cut(s) 573, 714
BspTI CTTAAG 3 cut(s) 374, 433, 466
BsrI ACTGG 4 cut(s) 79, 672, 909, 1044
BssECI CCNNGG 4 cut(s) 188, 256, 813, 1145
BssMI GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
BssT1I CCWWGG 2 cut(s) 256, 813
Bst4CI ACNGT 2 cut(s) 43, 1158
Bst6I CTCTTC 1 cut(s) 946
BstAFI CTTAAG 3 cut(s) 374, 433, 466
BstC8I GCNNGC 2 cut(s) 287, 1399
BstDEI CTNAG 5 cut(s) 102, 302, 535, 660, 720
BstF5I GGATG 1 cut(s) 1193
BstFNI CGCG 1 cut(s) 956
BstKTI GATC 7 cut(s) 204, 328, 568, 709, 772, 1307, 1370
BstMAI GTCTC 3 cut(s) 524, 579, 680
BstMBI GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
BstMWI GCNNNNNNNGC 2 cut(s) 444, 560
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 2 cut(s) 187, 188
BstUI CGCG 1 cut(s) 956
BstV1I GCAGC 1 cut(s) 1035
BstX2I RGATCY 4 cut(s) 565, 706, 1304, 1367
BstYI RGATCY 4 cut(s) 565, 706, 1304, 1367
BsuRI GGCC 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 1146
BtsCI GGATG 1 cut(s) 1193
BtsIMutI CAGTG 1 cut(s) 1037
Cac8I GCNNGC 2 cut(s) 287, 1399
CciI TCATGA 1 cut(s) 966
Cfr13I GGNCC 1 cut(s) 398
Cfr9I CCCGGG 1 cut(s) 188
Csp6I GTAC 3 cut(s) 39, 893, 1219
CviAII CATG 6 cut(s) 27, 479, 516, 967, 976, 1204
CviQI GTAC 3 cut(s) 39, 893, 1219
DdeI CTNAG 5 cut(s) 102, 302, 535, 660, 720
DpnI GATC 7 cut(s) 203, 327, 567, 708, 771, 1306, 1369
DpnII GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
Eam1104I CTCTTC 1 cut(s) 946
EarI CTCTTC 1 cut(s) 946
EciI GGCGGA 1 cut(s) 578
Eco130I CCWWGG 2 cut(s) 256, 813
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 2 cut(s) 188, 1166
EcoT14I CCWWGG 2 cut(s) 256, 813
ErhI CCWWGG 2 cut(s) 256, 813
FaeI CATG 6 cut(s) 30, 482, 519, 970, 979, 1207
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 1069
FatI CATG 6 cut(s) 26, 478, 515, 966, 975, 1203
FauNDI CATATG 3 cut(s) 628, 840, 1390
FbaI TGATCA 2 cut(s) 325, 769
FblI GTMKAC 2 cut(s) 870, 1335
Fnu4HI GCNGC 1 cut(s) 1049
FokI GGATG 1 cut(s) 1200
Fsp4HI GCNGC 1 cut(s) 1049
FspBI CTAG 8 cut(s) 144, 257, 267, 569, 741, 1247, 1362, 1371
GluI GCNGC 1 cut(s) 1049
HaeIII GGCC 1 cut(s) 1401
HapII CCGG 1 cut(s) 189
Hin1II CATG 6 cut(s) 30, 482, 519, 970, 979, 1207
HincII GTYRAC 2 cut(s) 871, 1336
HindII GTYRAC 2 cut(s) 871, 1336
HindIII AAGCTT 1 cut(s) 1066
HpaII CCGG 1 cut(s) 189
HphI GGTGA 3 cut(s) 329, 898, 978
Hpy166II GTNNAC 5 cut(s) 247, 640, 871, 1154, 1336
Hpy188I TCNGA 5 cut(s) 105, 123, 663, 721, 1116
Hpy8I GTNNAC 5 cut(s) 247, 640, 871, 1154, 1336
Hpy99I CGWCG 1 cut(s) 1337
HpyAV CCTTC 1 cut(s) 680
HpyCH4III ACNGT 2 cut(s) 43, 1158
HpyCH4IV ACGT 6 cut(s) 37, 343, 642, 1055, 1102, 1221
HpyCH4V TGCA 4 cut(s) 289, 655, 999, 1191
HpyF10VI GCNNNNNNNGC 2 cut(s) 444, 560
HpyF3I CTNAG 5 cut(s) 102, 302, 535, 660, 720
HpySE526I ACGT 6 cut(s) 37, 343, 642, 1055, 1102, 1221
Hsp92II CATG 6 cut(s) 30, 482, 519, 970, 979, 1207
Ksp22I TGATCA 2 cut(s) 325, 769
Kzo9I GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
Lsp1109I GCAGC 1 cut(s) 1035
LweI GCATC 3 cut(s) 388, 724, 1178
MaeI CTAG 8 cut(s) 144, 257, 267, 569, 741, 1247, 1362, 1371
MaeII ACGT 6 cut(s) 37, 343, 642, 1055, 1102, 1221
MaeIII GTNAC 6 cut(s) 81, 136, 337, 453, 1098, 1199
MalI GATC 7 cut(s) 203, 327, 567, 708, 771, 1306, 1369
MboI GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
MboII GAAGA 9 cut(s) 42, 110, 614, 815, 856, 933, 1299, 1331, 1377
MflI RGATCY 4 cut(s) 565, 706, 1304, 1367
MluCI AATT 6 cut(s) 898, 992, 1023, 1124, 1345, 1355
MlyI GAGTC 9 cut(s) 83, 178, 461, 509, 581, 738, 930, 1105, 1125
MmeI TCCRAC 2 cut(s) 326, 1204
MnlI CCTC 8 cut(s) 41, 58, 399, 553, 715, 1045, 1155, 1289
MroXI GAANNNNTTC 1 cut(s) 1068
MseI TTAA 6 cut(s) 375, 434, 467, 668, 809, 1127
MslI CAYNNNNRTG 1 cut(s) 384
MspCI CTTAAG 3 cut(s) 374, 433, 466
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 2 cut(s) 189, 190
Mva1269I GAATGC 1 cut(s) 306
MvnI CGCG 1 cut(s) 956
MwoI GCNNNNNNNGC 2 cut(s) 444, 560
NciI CCSGG 2 cut(s) 189, 190
NdeI CATATG 3 cut(s) 628, 840, 1390
NdeII GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
NlaIII CATG 6 cut(s) 30, 482, 519, 970, 979, 1207
NmuCI GTSAC 2 cut(s) 337, 453
NspI RCATGY 1 cut(s) 30
PaeR7I CTCGAG 1 cut(s) 1166
PagI TCATGA 1 cut(s) 966
PcsI WCGNNNNNNNCGW 1 cut(s) 1052
PctI GAATGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 1 cut(s) 757
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 1 cut(s) 1050
PleI GAGTC 9 cut(s) 83, 178, 460, 508, 580, 738, 929, 1105, 1125
PpsI GAGTC 9 cut(s) 83, 178, 460, 508, 580, 738, 929, 1105, 1125
Psp1406I AACGTT 2 cut(s) 642, 1055
PspPI GGNCC 1 cut(s) 398
PspXI VCTCGAGB 1 cut(s) 1166
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PsuI RGATCY 4 cut(s) 565, 706, 1304, 1367
RsaI GTAC 3 cut(s) 40, 894, 1220
RsaNI GTAC 3 cut(s) 39, 893, 1219
RseI CAYNNNNRTG 1 cut(s) 384
SalI GTCGAC 2 cut(s) 869, 1334
SaqAI TTAA 6 cut(s) 375, 434, 467, 668, 809, 1127
SatI GCNGC 1 cut(s) 1049
Sau3AI GATC 7 cut(s) 201, 325, 565, 706, 769, 1304, 1367
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 9 cut(s) 83, 178, 461, 509, 581, 738, 930, 1105, 1125
ScrFI CCNGG 2 cut(s) 189, 190
SfaNI GCATC 3 cut(s) 388, 724, 1178
Sfr274I CTCGAG 1 cut(s) 1166
SinI GGWCC 1 cut(s) 398
SlaI CTCGAG 1 cut(s) 1166
SmaI CCCGGG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 8 cut(s) 296, 374, 433, 466, 521, 746, 1079, 1166
SmoI CTYRAG 8 cut(s) 296, 374, 433, 466, 521, 746, 1079, 1166
SpeI ACTAGT 1 cut(s) 143
Sse9I AATT 6 cut(s) 898, 992, 1023, 1124, 1345, 1355
SsiI CCGC 1 cut(s) 563
SspMI CTAG 8 cut(s) 144, 257, 267, 569, 741, 1247, 1362, 1371
StyD4I CCNGG 2 cut(s) 187, 188
StyI CCWWGG 2 cut(s) 256, 813
TaaI ACNGT 2 cut(s) 43, 1158
TaiI ACGT 6 cut(s) 40, 346, 645, 1058, 1105, 1224
TaqI TCGA 5 cut(s) 33, 182, 870, 1167, 1335
TasI AATT 6 cut(s) 898, 992, 1023, 1124, 1345, 1355
TfiI GAWTC 1 cut(s) 757
Tru1I TTAA 6 cut(s) 375, 434, 467, 668, 809, 1127
Tru9I TTAA 6 cut(s) 375, 434, 467, 668, 809, 1127
TscAI CASTG 1 cut(s) 1044
TseFI GTSAC 2 cut(s) 337, 453
TseI GCWGC 1 cut(s) 1048
Tsp45I GTSAC 2 cut(s) 337, 453
TspDTI ATGAA 6 cut(s) 827, 857, 879, 947, 952, 983
TspGWI ACGGA 1 cut(s) 1321
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 1 cut(s) 1044
Van91I CCANNNNNTGG 1 cut(s) 162
Vha464I CTTAAG 3 cut(s) 374, 433, 466
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 4 cut(s) 992, 1023, 1345, 1355
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 1 cut(s) 30
XhoI CTCGAG 1 cut(s) 1166
XmaI CCCGGG 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 256
XmiI GTMKAC 2 cut(s) 870, 1335
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 8 cut(s) 144, 257, 267, 569, 741, 1247, 1362, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.