Rorug05G0108400

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000062
Physical Location & Seq
Reverse (-)
9604977 .. 9623427
18451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0108400.1

Sequence Viewer

Length: 3603 bp
ATGTTCGGCTTCCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGCCAACTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTGCACAGCTTTATCACAGCCCTGATCATTCTCAAGGTTTGGATTTTTTACTTTCTTTGTCAAGTTGGAAGCAGCAATTTCTGTCTCAATTTGAGTTTGAGGGTGCGGTGGTGTTTGCAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTACTAGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGACTCCCAACCCATCCCCGATGACAGTGTGCAATGGCCAATCACCTTATTCCTCCCCTTCTCCTTATCAAATCCTGTCTGCTCCTTCTAATACTTATAGACAACTCTATGAGAGGTTCCAAGGTGTCCATAATTCTCCTTTTCAAACCCTATCTGCCTCTCCTGTTACTCCTGTTCAACTTTATAAGAGCTTTCAAGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCATCACACTTAGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAAAAATTTGCCAATGGTAAACTTCGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCGTGGATGGGAGTTGCAAGTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGACGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTAGTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGTACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTCTATAAGTTAGCTGGCAAAACGATGCATGAAGGTAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACGGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAAGTAGGAACAAGAAGCAAGAGGGAGGAAAGGAAAAACAAAATTCTTCAGATCTCTACTCTGATTCCAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAGCCCTTATGAACATGGAAAATATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAAAGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAGTCTGTTGTTGGAAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCTGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATTGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCACAATGAGTGAGGCAGCTCAACACTGGATGGTTTGGATGGATAAATTTTTGAGTTTAGGAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATACTTCAATTGATTGATATTTACTATGAAGCTCTCGACGCATCCAAAAAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAAAAGTTCCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTTTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACATCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATTGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGGCGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACTGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCTCTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

1200

Amino Acids

135.94

Weight (kDa)

6.19

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2.6e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 305 - 369 3e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 392 - 1046 6e-94 RNA dependent RNA polymerase
RdRP_head PF26253 1074 - 1178 4.7e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13780 AT1G13780 AT1G16930 AT1G16930 AT1G16930 AT1G16940 AT1G16945 AT1G51370 AT1G51370 AT1G51370 AT1G60400 AT1G60410 AT1G66290 AT1G66300 AT1G66300 AT1G66310 AT1G66320 AT1G66640 AT1G69630 AT1G78730 AT1G78740 AT1G78750 AT1G78750 AT1G78760 AT1G78840 AT1G78840 AT1G80470 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G27505 AT3G19085 AT3G19085 AT3G23955 AT3G23955 AT4G13985 AT5G18770 AT5G18770 AT5G18780 AT5G18780 AT5G18780 AT5G18780 AT5G22590 AT5G22600 AT5G22600 AT5G22610 AT5G22610 AT5G22660 AT5G22660 AT5G22670 AT5G22680 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22720 AT5G22730 AT5G22730 AT5G22730 AT5G25850 AT5G27750 AT5G27750 AT5G44490 AT5G44490 AT5G44940 AT5G44950 AT5G44960 AT5G44970 AT5G44980 AT5G44980 AT5G44980 AT5G44980 AT5G53592 AT5G53635 AT5G53637 AT5G53640 AT5G53840
fragaria_vesca FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g01470 FvH4_1g01500 FvH4_1g06320 FvH4_1g06320 FvH4_1g09701 FvH4_1g09890 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_3g17060 FvH4_3g17061 FvH4_3g17061 FvH4_3g17061 FvH4_3g17080 FvH4_3g17080 FvH4_3g17080 FvH4_3g17090 FvH4_3g17100 FvH4_3g17110 FvH4_3g17130 FvH4_3g17160 FvH4_3g17160 FvH4_3g17161 FvH4_3g17190 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g21162 FvH4_3g21162 FvH4_3g21190 FvH4_4g14821 FvH4_4g14841 FvH4_4g14850 FvH4_5g35600 FvH4_6g23651 FvH4_6g38031
malus_domestica MD03G1246000.v1.1 MD03G1252900.v1.1 MD03G1253200.v1.1 MD03G1253500.v1.1 MD03G1253700.v1.1 MD08G1193400.v1.1 MD11G1256200.v1.1 MD11G1265900.v1.1 MD11G1266000.v1.1 MD11G1266700.v1.1 MD11G1266800.v1.1 MD11G1266900.v1.1 MD11G1267000.v1.1 MD11G1274900.v1.1 MD11G1275300.v1.1
prunus_persica Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158800_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G159000_v2.0.a1
pyrus_communis pycom03g19500 pycom03g20040 pycom03g20080 pycom03g20130 pycom03g20140 pycom08g16650 pycom11g22660 pycom11g23380 pycom11g23390 pycom11g23450 pycom11g23470 pycom11g23480 pycom11g23490 pycom11g24250
rosa_chinensis RchiOBHm_Chr1g0321381 RchiOBHm_Chr4g0417361 RchiOBHm_Chr4g0417391 RchiOBHm_Chr4g0417401 RchiOBHm_Chr4g0417411 RchiOBHm_Chr4g0417471 RchiOBHm_Chr5g0028391 RchiOBHm_Chr5g0028401 RchiOBHm_Chr5g0028441 RchiOBHm_Chr5g0028451 RchiOBHm_Chr5g0028461 RchiOBHm_Chr5g0028471 RchiOBHm_Chr5g0028481 RchiOBHm_Chr5g0028491 RchiOBHm_Chr5g0028501 RchiOBHm_Chr5g0028511 RchiOBHm_Chr5g0028521 RchiOBHm_Chr5g0028541 RchiOBHm_Chr5g0028551 RchiOBHm_Chr5g0028581 RchiOBHm_Chr5g0040081 RchiOBHm_Chr7g0192741 RchiOBHm_Chr7g0237071
rosa_laevigata RLG00000007914 RLG00000033090 RLG00000033092 RLG00000033096 RLG00000033097 RLG00000033098 RLG00000033099 RLG00000033100
rosa_multiflora Rmu_sc0000103.1_g000060 Rmu_sc0000272.1_g000005 Rmu_sc0000272.1_g000006 Rmu_sc0001134.1_g000002 Rmu_sc0001561.1_g000020 Rmu_sc0001561.1_g000021 Rmu_sc0001575.1_g000002 Rmu_sc0001575.1_g000007 Rmu_sc0001575.1_g000008 Rmu_sc0001575.1_g000010 Rmu_sc0001575.1_g000013 Rmu_sc0002131.1_g000038 Rmu_sc0004142.1_g000020 Rmu_sc0004142.1_g000021 Rmu_sc0004142.1_g000026 Rmu_sc0004142.1_g000027 Rmu_sc0004142.1_g000028 Rmu_sc0005594.1_g000010 Rmu_sc0006771.1_g000001 Rmu_sc0006771.1_g000002 Rmu_sc0006771.1_g000003 Rmu_sc0006771.1_g000004 Rmu_sc0006771.1_g000007 Rmu_sc0008526.1_g000014 Rmu_sc0009797.1_g000001
rosa_roxburghii Rroxscaffold_1G00041210 Rroxscaffold_1G00051210 Rroxscaffold_1G00051220 Rroxscaffold_1G00051230 Rroxscaffold_1G00051240 Rroxscaffold_1G00051280 Rroxscaffold_1G00051310 Rroxscaffold_3G00224520 Rroxscaffold_3G00224530 Rroxscaffold_5G00361120 Rroxscaffold_6G00412010
rosa_rugosa Rorug04G0147300 Rorug04G0147400 Rorug04G0147500 Rorug04G0147700 Rorug04G0147800 Rorug05G0107100 Rorug05G0107300 Rorug05G0107600 Rorug05G0107600 Rorug05G0107700 Rorug05G0107800 Rorug05G0107900 Rorug05G0108000 Rorug05G0108100 Rorug05G0108200 Rorug05G0108400 Rorug05G0108500 Rorug05G0108600 Rorug05G0108700 Rorug05G0108900 Rorug05G0109000 Rorug05G0109100 Rorug05G0109300 Rorug05G0109400 Rorug05G0109500 Rorug05G0183200 Rorug05G0183300 Rorug05G0183400 Rorug07G0187200 Rorug07G0301700 Rorug07G0301700 Rorug07G0301800
rosa_samantha Rh1AG413100 Rh1CG047800 Rh4AG210900 Rh4AG211000 Rh4AG211100 Rh4BG141000 Rh4BG211300 Rh4CG221700 Rh4CG221800 Rh4CG221900 Rh4DG208700 Rh4DG208800 Rh4DG208900 Rh5AG199800 Rh5AG200100 Rh5AG200200 Rh5AG200500 Rh5AG200600 Rh5AG200700 Rh5AG200800 Rh5AG200900 Rh5AG201000 Rh5AG201100 Rh5AG201300 Rh5AG201400 Rh5AG269900 Rh5BG198300 Rh5BG198500 Rh5BG198900 Rh5BG199000 Rh5BG199400 Rh5BG199500 Rh5BG199600 Rh5BG199700 Rh5BG199800 Rh5BG273400 Rh5CG218400 Rh5CG219200 Rh5CG219300 Rh5CG219400 Rh5CG219500 Rh5CG219700 Rh5CG219800 Rh5CG306300 Rh5CG306400 Rh5DG200300 Rh5DG200500 Rh5DG200600 Rh5DG201100 Rh5DG201200 Rh5DG201300 Rh5DG201400 Rh5DG201500 Rh5DG201600 Rh5DG201700 Rh5DG201800 Rh5DG201900 Rh5DG202100 Rh5DG202400 Rh5DG202500 Rh5DG203100 Rh5DG281500 Rh7AG457900 Rh7BG320900 Rh7BG428600 Rh7CG476000 Rh7DG445100 Rh7DG445200
rosa_wichuraiana Rw0G014200 Rw0G014210 Rw1G003950 Rw4G017810 Rw4G017820 Rw4G017850 Rw5G018220 Rw5G018230 Rw5G018250 Rw5G018260 Rw5G018270 Rw5G018280 Rw5G025230 Rw7G037950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
AarI CACCTGC 1 cut(s) 3497
Acc36I ACCTGC 1 cut(s) 3497
AccB1I GGYRCC 1 cut(s) 2179
AccB7I CCANNNNNTGG 1 cut(s) 1059
AccI GTMKAC 2 cut(s) 420, 1699
AciI CCGC 6 cut(s) 71, 401, 463, 1345, 2657, 3379
AclWI GGATC 9 cut(s) 51, 1740, 1802, 1994, 2009, 2444, 2708, 3127, 3334
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 9 cut(s) 144, 1027, 1253, 1475, 2029, 2089, 2409, 2912, 3174
AcuI CTGAAG 5 cut(s) 450, 540, 591, 2079, 2978
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1363
AfaI GTAC 7 cut(s) 619, 1303, 1367, 1533, 2528, 2544, 2618
AfiI CCNNNNNNNGG 6 cut(s) 83, 1059, 1164, 2176, 3304, 3349
AflII CTTAAG 1 cut(s) 59
AhlI ACTAGT 2 cut(s) 290, 614
AjnI CCWGG 5 cut(s) 508, 892, 1852, 2536, 3550
AjuI GAANNNNNNNTTGG 4 cut(s) 1068, 1100, 2785, 2817
AleI CACNNNNGTG 1 cut(s) 2550
Alw21I GWGCWC 4 cut(s) 479, 1451, 2829, 3469
Alw26I GTCTC 8 cut(s) 234, 384, 482, 653, 854, 1043, 1658, 2219
Alw44I GTGCAC 1 cut(s) 3465
AlwI GGATC 9 cut(s) 51, 1740, 1802, 1994, 2009, 2444, 2708, 3127, 3334
AlwNI CAGNNNCTG 1 cut(s) 1873
Ama87I CYCGRG 1 cut(s) 935
AoxI GGCC 3 cut(s) 694, 1541, 3295
ApaLI GTGCAC 1 cut(s) 3465
ApeKI GCWGC 8 cut(s) 31, 73, 367, 1457, 2255, 2882, 3308, 3512
ApoI RAATTY 9 cut(s) 144, 1027, 1253, 1475, 2029, 2089, 2409, 2912, 3174
Asp700I GAANNNNTTC 2 cut(s) 1329, 3059
AspLEI GCGC 2 cut(s) 1020, 3407
AspS9I GGNCC 7 cut(s) 139, 252, 905, 1184, 1541, 2642, 3295
AvaI CYCGRG 1 cut(s) 935
AvaII GGWCC 5 cut(s) 139, 252, 905, 1184, 2642
AxyI CCTNAGG 2 cut(s) 651, 3210
BaeGI GKGCMC 1 cut(s) 3469
BalI TGGCCA 1 cut(s) 696
BanI GGYRCC 1 cut(s) 2179
BanII GRGCYC 3 cut(s) 479, 1451, 2829
BarI GAAGNNNNNNTAC 2 cut(s) 3224, 3256
BauI CACGAG 1 cut(s) 2229
BbsI GAAGAC 4 cut(s) 952, 2852, 3162, 3540
Bbv12I GWGCWC 4 cut(s) 479, 1451, 2829, 3469
BbvCI CCTCAGC 2 cut(s) 2715, 3354
BbvI GCAGC 8 cut(s) 43, 85, 379, 1469, 2267, 2894, 3320, 3524
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 5 cut(s) 510, 894, 1854, 2538, 3552
BclI TGATCA 2 cut(s) 198, 319
BcoDI GTCTC 8 cut(s) 234, 384, 482, 653, 854, 1043, 1658, 2219
BcuI ACTAGT 2 cut(s) 290, 614
BfmI CTRYAG 1 cut(s) 1214
BfoI RGCGCY 1 cut(s) 1021
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 3497
BglII AGATCT 3 cut(s) 1514, 2098, 3161
BlpI GCTNAGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 5 cut(s) 510, 894, 1854, 2538, 3552
Bme18I GGWCC 5 cut(s) 139, 252, 905, 1184, 2642
BmeT110I CYCGRG 1 cut(s) 935
BmgT120I GGNCC 7 cut(s) 139, 252, 905, 1184, 1541, 2642, 3295
BmiI GGNNCC 7 cut(s) 254, 776, 1185, 1186, 1273, 2181, 3280
BmrFI CCNGG 5 cut(s) 510, 894, 1854, 2538, 3552
BmrI ACTGGG 2 cut(s) 872, 1548
BmsI GCATC 5 cut(s) 96, 613, 1444, 1491, 3017
BmuI ACTGGG 2 cut(s) 872, 1548
BoxI GACNNNNGTC 1 cut(s) 2506
BpiI GAAGAC 4 cut(s) 952, 2852, 3162, 3540
BpmI CTGGAG 3 cut(s) 993, 2057, 3559
Bpu10I CCTNAGC 2 cut(s) 2715, 3354
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 465, 1844, 2689
BsaAI YACGTR 3 cut(s) 133, 1531, 2577
BsaBI GATNNNNATC 2 cut(s) 1723, 2688
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 7 cut(s) 778, 901, 1158, 1261, 2170, 2747, 3273
BsaWI WCCGGW 2 cut(s) 2529, 3252
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 1059, 1164, 2176, 3304, 3349
Bse21I CCTNAGG 2 cut(s) 651, 3210
Bse3DI GCAATG 4 cut(s) 104, 186, 697, 1961
Bse8I GATNNNNATC 2 cut(s) 1723, 2688
BseBI CCWGG 5 cut(s) 510, 894, 1854, 2538, 3552
BseDI CCNNGG 7 cut(s) 778, 901, 1158, 1261, 2170, 2747, 3273
BseGI GGATG 8 cut(s) 671, 889, 1168, 2665, 2901, 2910, 3008, 3145
BseJI GATNNNNATC 2 cut(s) 1723, 2688
BseLI CCNNNNNNNGG 6 cut(s) 83, 1059, 1164, 2176, 3304, 3349
BseMI GCAATG 4 cut(s) 104, 186, 697, 1961
BseRI GAGGAG 1 cut(s) 3371
BseSI GKGCMC 1 cut(s) 3469
BseXI GCAGC 8 cut(s) 43, 85, 379, 1469, 2267, 2894, 3320, 3524
BsgI GTGCAG 4 cut(s) 50, 282, 2274, 3531
Bsh1285I CGRYCG 1 cut(s) 1976
BshFI GGCC 3 cut(s) 696, 1543, 3297
BshNI GGYRCC 1 cut(s) 2179
BsiEI CGRYCG 1 cut(s) 1976
BsiHKAI GWGCWC 4 cut(s) 479, 1451, 2829, 3469
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 3 cut(s) 77, 2530, 3253
BslFI GGGAC 4 cut(s) 249, 1170, 1812, 2455
BslI CCNNNNNNNGG 6 cut(s) 83, 1059, 1164, 2176, 3304, 3349
BsmAI GTCTC 8 cut(s) 234, 384, 482, 653, 854, 1043, 1658, 2219
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 4 cut(s) 249, 1170, 1812, 2455
BsmI GAATGC 2 cut(s) 1329, 2258
BsnI GGCC 3 cut(s) 696, 1543, 3297
Bso31I GGTCTC 1 cut(s) 1043
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 4 cut(s) 479, 1451, 2829, 3469
Bsp1407I TGTACA 2 cut(s) 1365, 2542
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 901, 2170, 3273
BspACI CCGC 6 cut(s) 71, 401, 463, 1345, 2657, 3379
BspANI GGCC 3 cut(s) 696, 1543, 3297
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 7 cut(s) 254, 776, 1185, 1186, 1273, 2181, 3280
BspMI ACCTGC 1 cut(s) 3497
BspPI GGATC 9 cut(s) 51, 1740, 1802, 1994, 2009, 2444, 2708, 3127, 3334
BspT107I GGYRCC 1 cut(s) 2179
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 4 cut(s) 104, 186, 697, 1961
BsrGI TGTACA 2 cut(s) 1365, 2542
BssECI CCNNGG 7 cut(s) 778, 901, 1158, 1261, 2170, 2747, 3273
BssNAI GTATAC 1 cut(s) 1700
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 2229
BssT1I CCWWGG 6 cut(s) 778, 901, 1261, 2170, 2747, 3273
Bst1107I GTATAC 1 cut(s) 1700
Bst2BI CACGAG 1 cut(s) 2229
Bst2UI CCWGG 5 cut(s) 510, 894, 1854, 2538, 3552
Bst6I CTCTTC 2 cut(s) 426, 2217
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 2 cut(s) 1365, 2542
BstBAI YACGTR 3 cut(s) 133, 1531, 2577
BstC8I GCNNGC 5 cut(s) 102, 116, 1065, 1492, 3524
BstDSI CCRYGG 4 cut(s) 901, 1158, 2170, 3273
BstEII GGTNACC 1 cut(s) 896
BstENI CCTNNNNNAGG 1 cut(s) 3347
BstF5I GGATG 8 cut(s) 671, 889, 1168, 2665, 2901, 2910, 3008, 3145
BstH2I RGCGCY 1 cut(s) 1021
BstHHI GCGC 2 cut(s) 1020, 3407
BstMAI GTCTC 8 cut(s) 234, 384, 482, 653, 854, 1043, 1658, 2219
BstMCI CGRYCG 1 cut(s) 1976
BstMWI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 3005, 3278, 3512
BstNI CCWGG 5 cut(s) 510, 894, 1854, 2538, 3552
BstNSI RCATGY 1 cut(s) 1067
BstPAI GACNNNNGTC 1 cut(s) 2506
BstPI GGTNACC 1 cut(s) 896
BstSCI CCNGG 5 cut(s) 508, 892, 1852, 2536, 3550
BstSFI CTRYAG 1 cut(s) 1214
BstSLI GKGCMC 1 cut(s) 3469
BstV1I GCAGC 8 cut(s) 43, 85, 379, 1469, 2267, 2894, 3320, 3524
BstV2I GAAGAC 4 cut(s) 952, 2852, 3162, 3540
BstX2I RGATCY 8 cut(s) 1514, 1732, 1999, 2014, 2098, 2700, 3161, 3339
BstYI RGATCY 8 cut(s) 1514, 1732, 1999, 2014, 2098, 2700, 3161, 3339
BstZ17I GTATAC 1 cut(s) 1700
Bsu36I CCTNAGG 2 cut(s) 651, 3210
BsuRI GGCC 3 cut(s) 696, 1543, 3297
BtgI CCRYGG 4 cut(s) 901, 1158, 2170, 3273
BtsCI GGATG 8 cut(s) 671, 889, 1168, 2665, 2901, 2910, 3008, 3145
BtsIMutI CAGTG 6 cut(s) 690, 1375, 1536, 2031, 2033, 2890
BveI ACCTGC 1 cut(s) 3497
Cac8I GCNNGC 5 cut(s) 102, 116, 1065, 1492, 3524
CaiI CAGNNNCTG 1 cut(s) 1873
CciI TCATGA 1 cut(s) 195
CfoI GCGC 2 cut(s) 1020, 3407
Cfr13I GGNCC 7 cut(s) 139, 252, 905, 1184, 1541, 2642, 3295
CseI GACGC 3 cut(s) 131, 1534, 3014
CsiI ACCWGGT 1 cut(s) 3550
Csp6I GTAC 7 cut(s) 618, 1302, 1366, 1532, 2527, 2543, 2617
CspCI CAANNNNNGTGG 4 cut(s) 1127, 1162, 3466, 3501
CviQI GTAC 7 cut(s) 618, 1302, 1366, 1532, 2527, 2543, 2617
DraI TTTAAA 1 cut(s) 2734
DraIII CACNNNGTG 1 cut(s) 1363
EaeI YGGCCR 1 cut(s) 694
Eam1104I CTCTTC 2 cut(s) 426, 2217
EarI CTCTTC 2 cut(s) 426, 2217
Ecl136II GAGCTC 3 cut(s) 477, 1449, 2827
Eco130I CCWWGG 6 cut(s) 778, 901, 1261, 2170, 2747, 3273
Eco24I GRGCYC 3 cut(s) 479, 1451, 2829
Eco31I GGTCTC 1 cut(s) 1043
Eco47I GGWCC 5 cut(s) 139, 252, 905, 1184, 2642
Eco53kI GAGCTC 3 cut(s) 477, 1449, 2827
Eco57I CTGAAG 5 cut(s) 450, 540, 591, 2079, 2978
Eco81I CCTNAGG 2 cut(s) 651, 3210
Eco88I CYCGRG 1 cut(s) 935
Eco91I GGTNACC 1 cut(s) 896
EcoICRI GAGCTC 3 cut(s) 477, 1449, 2827
EcoNI CCTNNNNNAGG 1 cut(s) 3347
EcoO109I RGGNCCY 1 cut(s) 1184
EcoO65I GGTNACC 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 2029
EcoRII CCWGG 5 cut(s) 508, 892, 1852, 2536, 3550
EcoT14I CCWWGG 6 cut(s) 778, 901, 1261, 2170, 2747, 3273
EcoT22I ATGCAT 1 cut(s) 1506
EcoT38I GRGCYC 3 cut(s) 479, 1451, 2829
ErhI CCWWGG 6 cut(s) 778, 901, 1261, 2170, 2747, 3273
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 8 cut(s) 1172, 1204, 1317, 1349, 1758, 1790, 2731, 2763
FaqI GGGAC 4 cut(s) 249, 1170, 1812, 2455
FauNDI CATATG 1 cut(s) 2347
FbaI TGATCA 2 cut(s) 198, 319
FblI GTMKAC 2 cut(s) 420, 1699
FokI GGATG 8 cut(s) 658, 876, 1175, 2672, 2908, 2917, 2995, 3152
FriOI GRGCYC 3 cut(s) 479, 1451, 2829
GlaI GCGC 2 cut(s) 1019, 3406
GsuI CTGGAG 3 cut(s) 993, 2057, 3559
HaeII RGCGCY 1 cut(s) 1021
HaeIII GGCC 3 cut(s) 696, 1543, 3297
HapII CCGG 3 cut(s) 77, 2530, 3253
HgaI GACGC 3 cut(s) 131, 1534, 3014
HhaI GCGC 2 cut(s) 1020, 3407
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 2 cut(s) 1018, 3405
HinP1I GCGC 2 cut(s) 1018, 3405
HincII GTYRAC 1 cut(s) 3202
HindII GTYRAC 1 cut(s) 3202
HindIII AAGCTT 3 cut(s) 573, 2214, 2863
HpaII CCGG 3 cut(s) 77, 2530, 3253
Hpy99I CGWCG 4 cut(s) 86, 1104, 2294, 3008
HpyCH4IV ACGT 7 cut(s) 132, 1530, 2251, 2576, 2647, 3204, 3423
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 3005, 3278, 3512
HpySE526I ACGT 7 cut(s) 132, 1530, 2251, 2576, 2647, 3204, 3423
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 2 cut(s) 1018, 3405
KflI GGGWCCC 1 cut(s) 1184
Ksp22I TGATCA 2 cut(s) 198, 319
LmnI GCTCC 2 cut(s) 745, 3278
Lsp1109I GCAGC 8 cut(s) 43, 85, 379, 1469, 2267, 2894, 3320, 3524
LweI GCATC 5 cut(s) 96, 613, 1444, 1491, 3017
MabI ACCWGGT 1 cut(s) 3550
MaeII ACGT 7 cut(s) 132, 1530, 2251, 2576, 2647, 3204, 3423
MfeI CAATTG 1 cut(s) 2975
MflI RGATCY 8 cut(s) 1514, 1732, 1999, 2014, 2098, 2700, 3161, 3339
MhlI GDGCHC 4 cut(s) 479, 1451, 2829, 3469
MlsI TGGCCA 1 cut(s) 696
MluNI TGGCCA 1 cut(s) 696
MlyI GAGTC 8 cut(s) 17, 271, 435, 655, 1670, 2221, 2603, 3579
MmeI TCCRAC 5 cut(s) 341, 1918, 2477, 2584, 2703
Mox20I TGGCCA 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 1506
MroXI GAANNNNTTC 2 cut(s) 1329, 3059
MscI TGGCCA 1 cut(s) 696
MseI TTAA 7 cut(s) 60, 962, 986, 1316, 2397, 2733, 3024
MslI CAYNNNNRTG 3 cut(s) 894, 1707, 2550
Msp20I TGGCCA 1 cut(s) 696
MspA1I CMGCKG 3 cut(s) 463, 2447, 2719
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 77, 2530, 3253
MspR9I CCNGG 5 cut(s) 510, 894, 1854, 2538, 3552
MssI GTTTAAAC 1 cut(s) 2734
MunI CAATTG 1 cut(s) 2975
Mva1269I GAATGC 2 cut(s) 1329, 2258
MvaI CCWGG 5 cut(s) 510, 894, 1854, 2538, 3552
MwoI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 3005, 3278, 3512
NcoI CCATGG 3 cut(s) 901, 2170, 3273
NdeI CATATG 1 cut(s) 2347
NlaIV GGNNCC 7 cut(s) 254, 776, 1185, 1186, 1273, 2181, 3280
NmuCI GTSAC 4 cut(s) 865, 896, 1355, 1614
NsiI ATGCAT 1 cut(s) 1506
NspI RCATGY 1 cut(s) 1067
OliI CACNNNNGTG 1 cut(s) 2550
PaeI GCATGC 1 cut(s) 1067
PaeR7I CTCGAG 1 cut(s) 935
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 3497
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 1329, 2258
PdmI GAANNNNTTC 2 cut(s) 1329, 3059
PfeI GAWTC 9 cut(s) 183, 218, 1121, 1969, 2111, 2423, 2533, 3092, 3301
PflMI CCANNNNNTGG 1 cut(s) 1059
PleI GAGTC 8 cut(s) 17, 270, 435, 655, 1669, 2221, 2602, 3578
PmeI GTTTAAAC 1 cut(s) 2734
PpsI GAGTC 8 cut(s) 17, 270, 435, 655, 1669, 2221, 2602, 3578
Ppu21I YACGTR 3 cut(s) 133, 1531, 2577
PpuMI RGGWCCY 1 cut(s) 1184
PshAI GACNNNNGTC 1 cut(s) 2506
PsiI TTATAA 1 cut(s) 843
Psp124BI GAGCTC 3 cut(s) 479, 1451, 2829
Psp5II RGGWCCY 1 cut(s) 1184
Psp6I CCWGG 5 cut(s) 508, 892, 1852, 2536, 3550
PspEI GGTNACC 1 cut(s) 896
PspGI CCWGG 5 cut(s) 508, 892, 1852, 2536, 3550
PspN4I GGNNCC 7 cut(s) 254, 776, 1185, 1186, 1273, 2181, 3280
PspPI GGNCC 7 cut(s) 139, 252, 905, 1184, 1541, 2642, 3295
PspPPI RGGWCCY 1 cut(s) 1184
PspXI VCTCGAGB 1 cut(s) 935
PstNI CAGNNNCTG 1 cut(s) 1873
PsuI RGATCY 8 cut(s) 1514, 1732, 1999, 2014, 2098, 2700, 3161, 3339
PvuII CAGCTG 2 cut(s) 2447, 2719
RsaI GTAC 7 cut(s) 619, 1303, 1367, 1533, 2528, 2544, 2618
RsaNI GTAC 7 cut(s) 618, 1302, 1366, 1532, 2527, 2543, 2617
RseI CAYNNNNRTG 3 cut(s) 894, 1707, 2550
SacI GAGCTC 3 cut(s) 479, 1451, 2829
SaqAI TTAA 7 cut(s) 60, 962, 986, 1316, 2397, 2733, 3024
Sau96I GGNCC 7 cut(s) 139, 252, 905, 1184, 1541, 2642, 3295
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 8 cut(s) 17, 271, 435, 655, 1670, 2221, 2603, 3579
ScrFI CCNGG 5 cut(s) 510, 894, 1854, 2538, 3552
SduI GDGCHC 4 cut(s) 479, 1451, 2829, 3469
SexAI ACCWGGT 1 cut(s) 3550
SfaNI GCATC 5 cut(s) 96, 613, 1444, 1491, 3017
SfcI CTRYAG 1 cut(s) 1214
Sfr274I CTCGAG 1 cut(s) 935
SinI GGWCC 5 cut(s) 139, 252, 905, 1184, 2642
SlaI CTCGAG 1 cut(s) 935
SmiMI CAYNNNNRTG 3 cut(s) 894, 1707, 2550
SmlI CTYRAG 6 cut(s) 59, 327, 480, 935, 1823, 2704
SmoI CTYRAG 6 cut(s) 59, 327, 480, 935, 1823, 2704
SpeI ACTAGT 2 cut(s) 290, 614
SphI GCATGC 1 cut(s) 1067
SsiI CCGC 6 cut(s) 71, 401, 463, 1345, 2657, 3379
SspI AATATT 2 cut(s) 960, 3316
SstI GAGCTC 3 cut(s) 479, 1451, 2829
StyD4I CCNGG 5 cut(s) 508, 892, 1852, 2536, 3550
StyI CCWWGG 6 cut(s) 778, 901, 1261, 2170, 2747, 3273
TaiI ACGT 7 cut(s) 135, 1533, 2254, 2579, 2650, 3207, 3426
TaqII GACCGA 1 cut(s) 1113
TatI WGTACW 3 cut(s) 617, 1365, 2542
TauI GCSGC 3 cut(s) 466, 1348, 3382
TfiI GAWTC 9 cut(s) 183, 218, 1121, 1969, 2111, 2423, 2533, 3092, 3301
Tru1I TTAA 7 cut(s) 60, 962, 986, 1316, 2397, 2733, 3024
Tru9I TTAA 7 cut(s) 60, 962, 986, 1316, 2397, 2733, 3024
TscAI CASTG 6 cut(s) 690, 1375, 1543, 2031, 2040, 2897
TseFI GTSAC 4 cut(s) 865, 896, 1355, 1614
TseI GCWGC 8 cut(s) 31, 73, 367, 1457, 2255, 2882, 3308, 3512
Tsp45I GTSAC 4 cut(s) 865, 896, 1355, 1614
TspGWI ACGGA 2 cut(s) 16, 1147
TspRI CASTG 6 cut(s) 690, 1375, 1543, 2031, 2040, 2897
Van91I CCANNNNNTGG 1 cut(s) 1059
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 3465
VpaK11BI GGWCC 5 cut(s) 139, 252, 905, 1184, 2642
XagI CCTNNNNNAGG 1 cut(s) 3347
XapI RAATTY 9 cut(s) 144, 1027, 1253, 1475, 2029, 2089, 2409, 2912, 3174
XbaI TCTAGA 2 cut(s) 239, 2815
XceI RCATGY 1 cut(s) 1067
XcmI CCANNNNNNNNNTGG 2 cut(s) 2745, 2777
XhoI CTCGAG 1 cut(s) 935
XmiI GTMKAC 2 cut(s) 420, 1699
XmnI GAANNNNTTC 2 cut(s) 1329, 3059
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.