AT5G25850

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000062
Physical Location & Seq
Forward (+)
9014082 .. 9015917
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25850.1

Sequence Viewer

Length: 1446 bp
ATGATGGGTAGAAAAAACTCAAAACAGGTTCGTTATTCCAATAGGTTGATACTCATAAATAGGTTAAGCCAATTATCTGATCCTTTGATATGTCAGATACTTTCTCATCTTCCCATAAAGGAGGTTGTCACGACAAGCGTTTTATCCACTAGATGGAAGAATATCTGGCTTAGTGTTCCTAGTTTGGAATTGATATATTCGATATTCCCAAATTTCAATACCTTTAGGAGTTTTGGTGATAGGTTTTTCGATTCCACTAGGGTTTCATGTATACACAATCTTAAGTTATATTTTGATGAACATGATGCATGTTATCTTACATCATGGATCAATGCCTTTGTTACACGTAACATCCGACGTCTATATGTCCGTCGTGTTCGTGGGAATTATTTTCATGAGCTGCCCTTAAGCCTTTATGTTTGTGAGACATTGGTGTCACTAAAACTCTTTCATCTGACTTTGGTTGACCCCGAATTTGTTTCTTTACCGTGTCTGAAAATCATGCATCTAAACTATGTTTGGTTTCCCAATGATGCCACTTTCGAGAGACTTGTCTCTTCTTGCCCTGTCTTAGAAGACTTAAAGATTGATGTATTATGGAATGATGGAAGAGTTTATCGAGTGAAATCTCGTTCACTTAAGAGACTCCGTTTACTACGAAGTTCTACTATGGTGTTTGATTCTGTTCCGGGTGTTGTTATTGATGCTCCTCTAATATGCTATTTGAGAATCAATGATCGTGTATCAGAAACCTATATATTAAATGATCTGGAGTCCAATGCCAAGTTAGATATACATCTAGACTTTGGTTCTGAAGATTTTGGCGAACCAAGCGTTTCATCGAGGAGAAGTCGCATCCGTAGTTTTCTGCCCGCAATATCGAAGGTCATGGGTTTGAAAATATCTGAGAACACTTTCAAGGTCATTCATCACTATTCCAATTTAGCACAACTACCTCAATTTGATAACACGTCCCGCTTGCACGTTACTCTCCGTGTATCCGAATTAAAATGGTTGCCGAATTTTCTTGAGTGTTGCCCGAATCTGAAATCCCTCATTGTGGCTTTTAATGGTGACTTTGAGAAGATGGGTTCTGAGGAGATGAATCAAATAAGTTTCCTTCTCCGGATGAAGAGGATTGTGGATATGACGATATTCATGTACACTTCCAAGGTATTGTTGCATAGTGATAACATAAATCAACTAAGTTTTTCATCTGTTCCTAAGTGTCTACTATCATCGCTCCAGTTTGTTGAACTCAATGCCCAAATCTTGAGATTTGATGGCGAAATACTGAATCTGGCGAAATACTTCTTAGAGAATTCATCTATCCTCCAAAAACTCACTCTACATCCGAACAAGTATGGTTCAACTTATGCGAATATGCTACACAAATTCCGAAGACGCTCTAGAACTTGCCAATTCATCCTAACTGATTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

481

Amino Acids

56.12

Weight (kDa)

9.3

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 61 4.9e-08 F-box domain
LRR_At1g61320_AtMIF1 PF23622 98 - 357 1.1e-07 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 107 - 239 1.1e-11 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 164 - 189 1.8e-09 Leucine Rich Repeat
FBD PF08387 405 - 450 5.8e-12 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13780 AT1G13780 AT1G16930 AT1G16930 AT1G16930 AT1G16940 AT1G16945 AT1G51370 AT1G51370 AT1G51370 AT1G60400 AT1G60410 AT1G66290 AT1G66300 AT1G66300 AT1G66310 AT1G66320 AT1G66640 AT1G69630 AT1G78730 AT1G78740 AT1G78750 AT1G78750 AT1G78760 AT1G78840 AT1G78840 AT1G80470 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G26030 AT2G27505 AT3G19085 AT3G19085 AT3G23955 AT3G23955 AT4G13985 AT5G18770 AT5G18770 AT5G18780 AT5G18780 AT5G18780 AT5G18780 AT5G22590 AT5G22600 AT5G22600 AT5G22610 AT5G22610 AT5G22660 AT5G22660 AT5G22670 AT5G22680 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22700 AT5G22720 AT5G22730 AT5G22730 AT5G22730 AT5G25850 AT5G27750 AT5G27750 AT5G44490 AT5G44490 AT5G44940 AT5G44950 AT5G44960 AT5G44970 AT5G44980 AT5G44980 AT5G44980 AT5G44980 AT5G53592 AT5G53635 AT5G53637 AT5G53640 AT5G53840
fragaria_vesca FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g00860 FvH4_1g01470 FvH4_1g01500 FvH4_1g06320 FvH4_1g06320 FvH4_1g09701 FvH4_1g09890 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_2g40800 FvH4_3g17060 FvH4_3g17061 FvH4_3g17061 FvH4_3g17061 FvH4_3g17080 FvH4_3g17080 FvH4_3g17080 FvH4_3g17090 FvH4_3g17100 FvH4_3g17110 FvH4_3g17130 FvH4_3g17160 FvH4_3g17160 FvH4_3g17161 FvH4_3g17190 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g18021 FvH4_3g21162 FvH4_3g21162 FvH4_3g21190 FvH4_4g14821 FvH4_4g14841 FvH4_4g14850 FvH4_5g35600 FvH4_6g23651 FvH4_6g38031
malus_domestica MD03G1246000.v1.1 MD03G1252900.v1.1 MD03G1253200.v1.1 MD03G1253500.v1.1 MD03G1253700.v1.1 MD08G1193400.v1.1 MD11G1256200.v1.1 MD11G1265900.v1.1 MD11G1266000.v1.1 MD11G1266700.v1.1 MD11G1266800.v1.1 MD11G1266900.v1.1 MD11G1267000.v1.1 MD11G1274900.v1.1 MD11G1275300.v1.1
prunus_persica Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158700_v2.0.a1 Prupe.4G158800_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G158900_v2.0.a1 Prupe.4G159000_v2.0.a1
pyrus_communis pycom03g19500 pycom03g20040 pycom03g20080 pycom03g20130 pycom03g20140 pycom08g16650 pycom11g22660 pycom11g23380 pycom11g23390 pycom11g23450 pycom11g23470 pycom11g23480 pycom11g23490 pycom11g24250
rosa_chinensis RchiOBHm_Chr1g0321381 RchiOBHm_Chr4g0417361 RchiOBHm_Chr4g0417391 RchiOBHm_Chr4g0417401 RchiOBHm_Chr4g0417411 RchiOBHm_Chr4g0417471 RchiOBHm_Chr5g0028391 RchiOBHm_Chr5g0028401 RchiOBHm_Chr5g0028441 RchiOBHm_Chr5g0028451 RchiOBHm_Chr5g0028461 RchiOBHm_Chr5g0028471 RchiOBHm_Chr5g0028481 RchiOBHm_Chr5g0028491 RchiOBHm_Chr5g0028501 RchiOBHm_Chr5g0028511 RchiOBHm_Chr5g0028521 RchiOBHm_Chr5g0028541 RchiOBHm_Chr5g0028551 RchiOBHm_Chr5g0028581 RchiOBHm_Chr5g0040081 RchiOBHm_Chr7g0192741 RchiOBHm_Chr7g0237071
rosa_laevigata RLG00000007914 RLG00000033090 RLG00000033092 RLG00000033096 RLG00000033097 RLG00000033098 RLG00000033099 RLG00000033100
rosa_multiflora Rmu_sc0000103.1_g000060 Rmu_sc0000272.1_g000005 Rmu_sc0000272.1_g000006 Rmu_sc0001134.1_g000002 Rmu_sc0001561.1_g000020 Rmu_sc0001561.1_g000021 Rmu_sc0001575.1_g000002 Rmu_sc0001575.1_g000007 Rmu_sc0001575.1_g000008 Rmu_sc0001575.1_g000010 Rmu_sc0001575.1_g000013 Rmu_sc0002131.1_g000038 Rmu_sc0004142.1_g000020 Rmu_sc0004142.1_g000021 Rmu_sc0004142.1_g000026 Rmu_sc0004142.1_g000027 Rmu_sc0004142.1_g000028 Rmu_sc0005594.1_g000010 Rmu_sc0006771.1_g000001 Rmu_sc0006771.1_g000002 Rmu_sc0006771.1_g000003 Rmu_sc0006771.1_g000004 Rmu_sc0006771.1_g000007 Rmu_sc0008526.1_g000014 Rmu_sc0009797.1_g000001
rosa_roxburghii Rroxscaffold_1G00041210 Rroxscaffold_1G00051210 Rroxscaffold_1G00051220 Rroxscaffold_1G00051230 Rroxscaffold_1G00051240 Rroxscaffold_1G00051280 Rroxscaffold_1G00051310 Rroxscaffold_3G00224520 Rroxscaffold_3G00224530 Rroxscaffold_5G00361120 Rroxscaffold_6G00412010
rosa_rugosa Rorug04G0147300 Rorug04G0147400 Rorug04G0147500 Rorug04G0147700 Rorug04G0147800 Rorug05G0107100 Rorug05G0107300 Rorug05G0107600 Rorug05G0107600 Rorug05G0107700 Rorug05G0107800 Rorug05G0107900 Rorug05G0108000 Rorug05G0108100 Rorug05G0108200 Rorug05G0108400 Rorug05G0108500 Rorug05G0108600 Rorug05G0108700 Rorug05G0108900 Rorug05G0109000 Rorug05G0109100 Rorug05G0109300 Rorug05G0109400 Rorug05G0109500 Rorug05G0183200 Rorug05G0183300 Rorug05G0183400 Rorug07G0187200 Rorug07G0301700 Rorug07G0301700 Rorug07G0301800
rosa_samantha Rh1AG413100 Rh1CG047800 Rh4AG210900 Rh4AG211000 Rh4AG211100 Rh4BG141000 Rh4BG211300 Rh4CG221700 Rh4CG221800 Rh4CG221900 Rh4DG208700 Rh4DG208800 Rh4DG208900 Rh5AG199800 Rh5AG200100 Rh5AG200200 Rh5AG200500 Rh5AG200600 Rh5AG200700 Rh5AG200800 Rh5AG200900 Rh5AG201000 Rh5AG201100 Rh5AG201300 Rh5AG201400 Rh5AG269900 Rh5BG198300 Rh5BG198500 Rh5BG198900 Rh5BG199000 Rh5BG199400 Rh5BG199500 Rh5BG199600 Rh5BG199700 Rh5BG199800 Rh5BG273400 Rh5CG218400 Rh5CG219200 Rh5CG219300 Rh5CG219400 Rh5CG219500 Rh5CG219700 Rh5CG219800 Rh5CG306300 Rh5CG306400 Rh5DG200300 Rh5DG200500 Rh5DG200600 Rh5DG201100 Rh5DG201200 Rh5DG201300 Rh5DG201400 Rh5DG201500 Rh5DG201600 Rh5DG201700 Rh5DG201800 Rh5DG201900 Rh5DG202100 Rh5DG202400 Rh5DG202500 Rh5DG203100 Rh5DG281500 Rh7AG457900 Rh7BG320900 Rh7BG428600 Rh7CG476000 Rh7DG445100 Rh7DG445200
rosa_wichuraiana Rw0G014200 Rw0G014210 Rw1G003950 Rw4G017810 Rw4G017820 Rw4G017850 Rw5G018220 Rw5G018230 Rw5G018250 Rw5G018260 Rw5G018270 Rw5G018280 Rw5G025230 Rw7G037950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AatII GACGTC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 153
AccI GTMKAC 2 cut(s) 271, 1231
AccIII TCCGGA 1 cut(s) 1125
AciI CCGC 2 cut(s) 873, 976
AclWI GGATC 2 cut(s) 74, 335
AcsI RAATTY 5 cut(s) 211, 473, 1021, 1321, 1394
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 1 cut(s) 358
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 2 cut(s) 153, 1060
AflII CTTAAG 3 cut(s) 281, 406, 638
AflIII ACRYGT 2 cut(s) 344, 969
AgsI TTSAA 5 cut(s) 217, 898, 919, 1256, 1371
AjiI CACGTC 1 cut(s) 972
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
Alw26I GTCTC 4 cut(s) 419, 541, 559, 637
AlwI GGATC 2 cut(s) 74, 335
Aor13HI TCCGGA 1 cut(s) 1125
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 5 cut(s) 211, 473, 1021, 1321, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp700I GAANNNNTTC 2 cut(s) 914, 1310
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 2 cut(s) 248, 1085
BbsI GAAGAC 2 cut(s) 582, 1408
BbvI GCAGC 1 cut(s) 387
BccI CCATC 4 cut(s) 147, 599, 1081, 1277
BciVI GTATCC 1 cut(s) 1009
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 4 cut(s) 419, 541, 559, 637
BfaI CTAG 5 cut(s) 150, 180, 258, 800, 1410
BfrI CTTAAG 3 cut(s) 281, 406, 638
BfuI GTATCC 1 cut(s) 1009
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 690
BmgBI CACGTC 1 cut(s) 972
BmrFI CCNGG 1 cut(s) 690
BmsI GCATC 5 cut(s) 295, 514, 523, 694, 864
BpiI GAAGAC 2 cut(s) 582, 1408
BpmI CTGGAG 2 cut(s) 791, 1229
BpuEI CTTGAG 2 cut(s) 1049, 1294
BpuMI CCSGG 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 1170
BsaWI WCCGGW 1 cut(s) 1125
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 1060
Bse1I ACTGG 1 cut(s) 1246
Bse8I GATNNNNATC 1 cut(s) 795
BseAI TCCGGA 1 cut(s) 1125
BseDI CCNNGG 1 cut(s) 1170
BseGI GGATG 5 cut(s) 351, 855, 1134, 1351, 1425
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 2 cut(s) 153, 1060
BseMII CTCAG 2 cut(s) 897, 1086
BseNI ACTGG 1 cut(s) 1246
BseRI GAGGAG 3 cut(s) 699, 859, 1112
BseXI GCAGC 1 cut(s) 387
BsiSI CCGG 2 cut(s) 689, 1126
BslFI GGGAC 1 cut(s) 958
BslI CCNNNNNNNGG 2 cut(s) 153, 1060
BsmAI GTCTC 4 cut(s) 419, 541, 559, 637
BsmFI GGGAC 1 cut(s) 958
Bsp13I TCCGGA 1 cut(s) 1125
Bsp1407I TGTACA 1 cut(s) 1161
Bsp143I GATC 4 cut(s) 79, 327, 736, 766
BspACI CCGC 2 cut(s) 873, 976
BspCNI CTCAG 2 cut(s) 898, 1087
BspEI TCCGGA 1 cut(s) 1125
BspHI TCATGA 1 cut(s) 394
BspPI GGATC 2 cut(s) 74, 335
BspTI CTTAAG 3 cut(s) 281, 406, 638
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 1 cut(s) 1246
BssECI CCNNGG 1 cut(s) 1170
BssMI GATC 4 cut(s) 79, 327, 736, 766
BssNAI GTATAC 1 cut(s) 272
BssNI GRCGYC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 1170
Bst1107I GTATAC 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 3 cut(s) 562, 604, 1127
BstACI GRCGYC 1 cut(s) 358
BstAFI CTTAAG 3 cut(s) 281, 406, 638
BstAUI TGTACA 1 cut(s) 1161
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 873, 980
BstDEI CTNAG 7 cut(s) 170, 571, 906, 1095, 1205, 1224, 1315
BstF5I GGATG 5 cut(s) 351, 855, 1134, 1351, 1425
BstKTI GATC 4 cut(s) 82, 330, 739, 769
BstMAI GTCTC 4 cut(s) 419, 541, 559, 637
BstMBI GATC 4 cut(s) 79, 327, 736, 766
BstMWI GCNNNNNNNGC 1 cut(s) 831
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 688
BstV1I GCAGC 1 cut(s) 387
BstV2I GAAGAC 2 cut(s) 582, 1408
BstZ17I GTATAC 1 cut(s) 272
BsuI GTATCC 1 cut(s) 1009
BtgZI GCGATG 1 cut(s) 1224
BtrI CACGTC 1 cut(s) 972
BtsCI GGATG 5 cut(s) 351, 855, 1134, 1351, 1425
Cac8I GCNNGC 2 cut(s) 873, 980
CciI TCATGA 1 cut(s) 394
CseI GACGC 1 cut(s) 1413
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 8 cut(s) 267, 302, 309, 324, 395, 502, 889, 1159
CviJI RGCY 5 cut(s) 69, 169, 400, 411, 1064
CviKI_1 RGCY 5 cut(s) 69, 169, 400, 411, 1064
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 7 cut(s) 170, 571, 906, 1095, 1205, 1224, 1315
DpnI GATC 4 cut(s) 81, 329, 738, 768
DpnII GATC 4 cut(s) 79, 327, 736, 766
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 3 cut(s) 562, 604, 1127
EarI CTCTTC 3 cut(s) 562, 604, 1127
Eco130I CCWWGG 1 cut(s) 1170
Eco57I CTGAAG 1 cut(s) 834
EcoRI GAATTC 1 cut(s) 1321
EcoT14I CCWWGG 1 cut(s) 1170
EcoT22I ATGCAT 2 cut(s) 310, 507
ErhI CCWWGG 1 cut(s) 1170
FaeI CATG 8 cut(s) 270, 305, 312, 327, 398, 505, 892, 1162
FaqI GGGAC 1 cut(s) 958
FatI CATG 8 cut(s) 266, 301, 308, 323, 394, 501, 888, 1158
FauI CCCGC 2 cut(s) 880, 983
FblI GTMKAC 2 cut(s) 271, 1231
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 5 cut(s) 338, 842, 1141, 1338, 1412
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 5 cut(s) 150, 180, 258, 800, 1410
GluI GCNGC 1 cut(s) 401
GsuI CTGGAG 2 cut(s) 791, 1229
HapII CCGG 2 cut(s) 689, 1126
HgaI GACGC 1 cut(s) 1413
Hin1I GRCGYC 1 cut(s) 358
Hin1II CATG 8 cut(s) 270, 305, 312, 327, 398, 505, 892, 1162
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 8 cut(s) 251, 645, 680, 729, 773, 1042, 1105, 1297
HpaII CCGG 2 cut(s) 689, 1126
HphI GGTGA 2 cut(s) 248, 1085
Hpy166II GTNNAC 6 cut(s) 272, 466, 635, 653, 1164, 1232
Hpy188III TCNNGA 9 cut(s) 130, 395, 544, 770, 800, 1028, 1126, 1273, 1410
Hpy8I GTNNAC 6 cut(s) 272, 466, 635, 653, 1164, 1232
Hpy99I CGWCG 2 cut(s) 360, 375
HpyAV CCTTC 2 cut(s) 877, 1130
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4IV ACGT 4 cut(s) 346, 358, 971, 984
HpyCH4V TGCA 4 cut(s) 308, 505, 982, 1183
HpyF10VI GCNNNNNNNGC 1 cut(s) 831
HpyF3I CTNAG 7 cut(s) 170, 571, 906, 1095, 1205, 1224, 1315
HpySE526I ACGT 4 cut(s) 346, 358, 971, 984
Hsp92I GRCGYC 1 cut(s) 358
Hsp92II CATG 8 cut(s) 270, 305, 312, 327, 398, 505, 892, 1162
Kpn2I TCCGGA 1 cut(s) 1125
Kzo9I GATC 4 cut(s) 79, 327, 736, 766
LmnI GCTCC 2 cut(s) 712, 1248
LpnPI CCDG 8 cut(s) 11, 151, 579, 702, 755, 1139, 1259, 1286
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 5 cut(s) 295, 514, 523, 694, 864
MaeI CTAG 5 cut(s) 150, 180, 258, 800, 1410
MaeII ACGT 4 cut(s) 346, 358, 971, 984
MaeIII GTNAC 6 cut(s) 127, 340, 347, 435, 985, 1073
MalI GATC 4 cut(s) 81, 329, 738, 768
MboI GATC 4 cut(s) 79, 327, 736, 766
MboII GAAGA 9 cut(s) 101, 169, 549, 587, 621, 827, 1096, 1144, 1413
MlyI GAGTC 2 cut(s) 639, 782
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 8 cut(s) 115, 720, 837, 966, 1064, 1090, 1128, 1343
Mph1103I ATGCAT 2 cut(s) 310, 507
MroI TCCGGA 1 cut(s) 1125
MroXI GAANNNNTTC 2 cut(s) 914, 1310
MseI TTAA 8 cut(s) 65, 282, 407, 581, 639, 761, 1007, 1068
MspCI CTTAAG 3 cut(s) 281, 406, 638
MspI CCGG 2 cut(s) 689, 1126
MspR9I CCNGG 1 cut(s) 690
MwoI GCNNNNNNNGC 1 cut(s) 831
NciI CCSGG 1 cut(s) 690
NdeII GATC 4 cut(s) 79, 327, 736, 766
NlaIII CATG 8 cut(s) 270, 305, 312, 327, 398, 505, 892, 1162
NmuCI GTSAC 3 cut(s) 127, 435, 1073
NsiI ATGCAT 2 cut(s) 310, 507
NspI RCATGY 1 cut(s) 312
PagI TCATGA 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 352
PdmI GAANNNNTTC 2 cut(s) 914, 1310
PfeI GAWTC 6 cut(s) 251, 680, 729, 1042, 1105, 1297
PflMI CCANNNNNTGG 1 cut(s) 153
PkrI GCNGC 1 cut(s) 402
PleI GAGTC 2 cut(s) 639, 781
PpsI GAGTC 2 cut(s) 639, 781
Ppu21I YACGTR 1 cut(s) 347
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
SaqAI TTAA 8 cut(s) 65, 282, 407, 581, 639, 761, 1007, 1068
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 4 cut(s) 79, 327, 736, 766
SchI GAGTC 2 cut(s) 639, 782
ScrFI CCNGG 1 cut(s) 690
SfaNI GCATC 5 cut(s) 295, 514, 523, 694, 864
SmlI CTYRAG 5 cut(s) 281, 406, 638, 1028, 1273
SmoI CTYRAG 5 cut(s) 281, 406, 638, 1028, 1273
SsiI CCGC 2 cut(s) 873, 976
SspMI CTAG 5 cut(s) 150, 180, 258, 800, 1410
StyD4I CCNGG 1 cut(s) 688
StyI CCWWGG 1 cut(s) 1170
TaaI ACNGT 1 cut(s) 489
TaiI ACGT 4 cut(s) 349, 361, 974, 987
TaqI TCGA 6 cut(s) 200, 249, 543, 619, 842, 881
TatI WGTACW 1 cut(s) 1161
TfiI GAWTC 6 cut(s) 251, 680, 729, 1042, 1105, 1297
Tru1I TTAA 8 cut(s) 65, 282, 407, 581, 639, 761, 1007, 1068
Tru9I TTAA 8 cut(s) 65, 282, 407, 581, 639, 761, 1007, 1068
TseFI GTSAC 3 cut(s) 127, 435, 1073
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 3 cut(s) 127, 435, 1073
TspGWI ACGGA 4 cut(s) 359, 638, 848, 983
Van91I CCANNNNNTGG 1 cut(s) 153
Vha464I CTTAAG 3 cut(s) 281, 406, 638
XapI RAATTY 5 cut(s) 211, 473, 1021, 1321, 1394
XbaI TCTAGA 2 cut(s) 799, 1409
XceI RCATGY 1 cut(s) 312
XmiI GTMKAC 2 cut(s) 271, 1231
XmnI GAANNNNTTC 2 cut(s) 914, 1310
XspI CTAG 5 cut(s) 150, 180, 258, 800, 1410
ZraI GACGTC 1 cut(s) 359
Zsp2I ATGCAT 2 cut(s) 310, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.