FvH4_1g00570

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000125
Physical Location & Seq
Reverse (-)
306927 .. 308251
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00570.t1

Sequence Viewer

Length: 942 bp
ATGAAGAGCCACAGAAGGATCCGCCACAGAAAGTTGAATTTGATGGAGCTGCTACCCATGGAACTCCTGGTGGACATCCTAAAGAGAGTGTCTGCAGAGTCTGTGTGTCGCATCCGATGCGTCTCTAAGATGTTGTTAAGCATAGTCGACTCCCCCTCTTTTGTTAAGCTGCACTCTACTTTGGTATTTGATCCAGATTCTCGTGTAACACCCGCTCCACCTCAATTCATCCTGCATTATGGCCTATCACGCTTTTATTCTCGCCGAGAAAACATGCTATCCTCCTTTCATTCACTTCACTACGACCCTTCCAATGCCCAAGGTGCTCCTCAACTTAATCCAAGACTTATTTTGCAGTCATCCAATCCCACATATTACAGTCTACGGTTTGTGTTCTGTAACATGTTTTGCATCAAATACTGGAGCGATCATGCACTCAGCTTCTTGATCAATCCTCTTCGAGGAGAAGTTCTAAAGCTCCCACCCAGTCACAGTATTGCTCAACAAATTTTTGGTCCATATTCCGAAGCAAGGGATGTTTCGACTGGTACGACTATTGAGATATGGGTGATGAAAGACTACATTAAAAAACAGTGGATGTTGGATTATTCCATCAGTATTCAAGTGCTTGAATTAGATCCTCAATTTAAGTTTTCGGAAGCTGTTTGTTGTGAATGGGAGCATGGCATACTTTTCACACATTCATCGGCCACAATAACATTGTTTTTGGATATGAGAGGACCTGTTGTTACCAAGACTCTTGTCAAATGTCCAATTGAGGGTAATTTGGTGCAAAGGCATATCATAAGTCTTCATGGGAGCTTGATTTCCCTGAAGAGCTTGGCAAATTTGGAGGAAGCAGAAGAGCCAGCTACTTATAGCGATTGGTCTGAAGGAGTAGCAAGAGGGAAGAATTTCTTTTATTTGACAAGGCGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

314

Amino Acids

36.05

Weight (kDa)

8.9

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 17 - 55 1.2e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48550 AT5G48550
fragaria_vesca FvH4_1g00551 FvH4_1g00570 FvH4_1g00691 FvH4_1g00700 FvH4_1g00812 FvH4_1g20161 FvH4_1g25190 FvH4_2g04190 FvH4_2g20921 FvH4_2g25871 FvH4_3g13181 FvH4_3g19662 FvH4_3g40671 FvH4_4g22410 FvH4_4g34520 FvH4_4g34580 FvH4_5g06780 FvH4_5g07350 FvH4_5g07470 FvH4_5g07510 FvH4_5g08848 FvH4_5g31171 FvH4_5g38320 FvH4_6g03511 FvH4_6g09071 FvH4_6g09550 FvH4_6g09801 FvH4_6g09911 FvH4_6g14781 FvH4_6g22690 FvH4_6g45801 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47471 FvH4_6g47480
malus_domestica MD04G1179800.v1.1 MD11G1005600.v1.1 MD11G1006200.v1.1 MD11G1006300.v1.1 MD11G1006900.v1.1 MD12G1195000.v1.1 MD12G1195500.v1.1 MD12G1195600.v1.1
prunus_persica Prupe.2G095600_v2.0.a1 Prupe.2G095700_v2.0.a1 Prupe.2G095800_v2.0.a1 Prupe.2G095900_v2.0.a1 Prupe.2G096000_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G209200_v2.0.a1 Prupe.3G238400_v2.0.a1 Prupe.7G182200_v2.0.a1 Prupe.7G194900_v2.0.a1 Prupe.7G195100_v2.0.a1 Prupe.7G195400_v2.0.a1 Prupe.8G061400_v2.0.a1
pyrus_communis pycom01g13020 pycom04g15890 pycom09g19490 pycom09g19510 pycom10g28160 pycom12g18120 pycom12g18180
rosa_chinensis RchiOBHm_Chr2g0085201 RchiOBHm_Chr2g0164001 RchiOBHm_Chr3g0449831 RchiOBHm_Chr3g0460411 RchiOBHm_Chr3g0480281 RchiOBHm_Chr4g0428611 RchiOBHm_Chr5g0016491 RchiOBHm_Chr6g0253541
rosa_laevigata RLG00000004425 RLG00000004426 RLG00000005189 RLG00000005350 RLG00000007140 RLG00000015004 RLG00000015666 RLG00000015801 RLG00000015804 RLG00000015807 RLG00000018680 RLG00000021432 RLG00000021625 RLG00000021626 RLG00000023498 RLG00000023500 RLG00000023502 RLG00000023505 RLG00000025014 RLG00000025039 RLG00000025070 RLG00000025093 RLG00000025094 RLG00000025791 RLG00000026900 RLG00000031177
rosa_multiflora Rmu_co8452909.1_g000001 Rmu_co8488687.1_g000001 Rmu_sc0000610.1_g000006 Rmu_sc0000770.1_g000026 Rmu_sc0001755.1_g000003 Rmu_sc0002072.1_g000002 Rmu_sc0002676.1_g000002 Rmu_sc0003133.1_g000022 Rmu_sc0003797.1_g000014 Rmu_sc0003901.1_g000002 Rmu_sc0003901.1_g000004 Rmu_sc0003906.1_g000004 Rmu_sc0004212.1_g000002 Rmu_sc0004621.1_g000026 Rmu_sc0006704.1_g000010 Rmu_sc0006944.1_g000006 Rmu_sc0008647.1_g000003 Rmu_sc0010698.1_g000005 Rmu_sc0010842.1_g000001 Rmu_sc0010911.1_g000004 Rmu_sc0011989.1_g000005 Rmu_sc0013983.1_g000002 Rmu_sc0016910.1_g000001 Rmu_sc0017276.1_g000001 Rmu_sc0020812.1_g000004 Rmu_sc0024877.1_g000001 Rmu_sc0025932.1_g000003 Rmu_ssc0000432.1_g000018
rosa_roxburghii Rroxscaffold_1G00072180 Rroxscaffold_1G00073060 Rroxscaffold_1G00074760 Rroxscaffold_2G00084790 Rroxscaffold_2G00086850 Rroxscaffold_2G00121570 Rroxscaffold_2G00154120 Rroxscaffold_3G00263530 Rroxscaffold_3G00273960 Rroxscaffold_4G00284960 Rroxscaffold_4G00284970 Rroxscaffold_5G00370300 Rroxscaffold_6G00401620 Rroxscaffold_6G00401630 Rroxscaffold_6G00401640 Rroxscaffold_6G00416730 Rroxscaffold_6G00419520 Rroxscaffold_6G00419780 Rroxscaffold_6G00420290 Rroxscaffold_6G00427650 Rroxscaffold_7G00176150 Rroxscaffold_7G00211790
rosa_rugosa Rorug02G0239000
rosa_samantha Rh1DG375000 Rh2AG006800 Rh2AG009900 Rh2AG024700 Rh2AG297700 Rh2AG574100 Rh2AG589700 Rh2AG589800 Rh2DG008500 Rh2DG012500 Rh2DG025200 Rh2DG321300 Rh2DG594200 Rh2DG612500 Rh3AG021800 Rh3AG093100 Rh3AG095100 Rh3AG095700 Rh3AG233400 Rh3AG233500 Rh3AG233600 Rh3CG021300 Rh3CG097200 Rh3CG097300 Rh3CG099400 Rh3CG100200 Rh3CG103500 Rh3CG103600 Rh3CG106200 Rh3CG263600 Rh3CG263700 Rh4DG287300 Rh5AG034300 Rh5AG495600 Rh5DG032900 Rh5DG033000 Rh5DG051400 Rh5DG532200 Rh6BG054100 Rh6BG337400 Rh6CG053800 Rh6CG343700 Rh6CG343800 Rh7AG043600 Rh7AG043800 Rh7AG121200 Rh7AG121300 Rh7DG025000 Rh7DG043300 Rh7DG124500 Rh7DG124600
rosa_wichuraiana Rw1G033430 Rw2G000640 Rw2G001940 Rw2G001990 Rw2G023800 Rw2G047500 Rw2G049160 Rw3G008010 Rw3G008540 Rw3G021000 Rw4G024660 Rw5G003140 Rw6G005370 Rw7G041040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 215
AccI GTMKAC 2 cut(s) 147, 382
AciI CCGC 3 cut(s) 22, 213, 934
AclWI GGATC 4 cut(s) 13, 26, 185, 632
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 4 cut(s) 37, 507, 847, 913
AcuI CTGAAG 2 cut(s) 854, 912
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 461, 531, 779
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 37, 623, 632
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 8 cut(s) 49, 169, 441, 478, 662, 822, 840, 872
AluI AGCT 8 cut(s) 49, 169, 441, 478, 662, 822, 840, 872
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 4 cut(s) 13, 26, 185, 632
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 2 cut(s) 241, 708
ApeKI GCWGC 2 cut(s) 49, 169
ApoI RAATTY 4 cut(s) 37, 507, 847, 913
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
Asp700I GAANNNNTTC 1 cut(s) 914
AspS9I GGNCC 2 cut(s) 515, 740
AsuHPI GGTGA 1 cut(s) 580
AvaII GGWCC 2 cut(s) 515, 740
BamHI GGATCC 1 cut(s) 18
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 2 cut(s) 36, 156
BccI CCATC 2 cut(s) 37, 620
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 127
BfmI CTRYAG 1 cut(s) 93
BisI GCNGC 3 cut(s) 50, 170, 935
BlsI GCNGC 3 cut(s) 51, 171, 936
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 2 cut(s) 515, 740
BmgT120I GGNCC 2 cut(s) 515, 740
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 1 cut(s) 68
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 3 cut(s) 107, 120, 420
BmuI ACTGGG 1 cut(s) 480
BoxI GACNNNNGTC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 803
BpmI CTGGAG 1 cut(s) 442
BsaJI CCNNGG 2 cut(s) 57, 319
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 531, 779
Bse1I ACTGG 3 cut(s) 425, 486, 550
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 57, 319
BseGI GGATG 6 cut(s) 75, 111, 228, 359, 541, 603
BseLI CCNNNNNNNGG 3 cut(s) 461, 531, 779
BseMII CTCAG 1 cut(s) 451
BseNI ACTGG 3 cut(s) 425, 486, 550
BseRI GAGGAG 2 cut(s) 318, 477
BseXI GCAGC 2 cut(s) 36, 156
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 2 cut(s) 243, 710
BsiHKAI GWGCWC 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 461, 531, 779
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 2 cut(s) 243, 710
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 5 cut(s) 18, 190, 427, 447, 637
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 22, 213, 934
BspANI GGCC 2 cut(s) 243, 710
BspCNI CTCAG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 20
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 4 cut(s) 13, 26, 185, 632
BspQI GCTCTTC 2 cut(s) 830, 858
BsrBI CCGCTC 1 cut(s) 215
BsrI ACTGG 3 cut(s) 425, 486, 550
BssECI CCNNGG 2 cut(s) 57, 319
BssMI GATC 5 cut(s) 18, 190, 427, 447, 637
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 57, 319
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 4 cut(s) 380, 387, 494, 594
Bst6I CTCTTC 3 cut(s) 462, 830, 858
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 870
BstDEI CTNAG 2 cut(s) 126, 437
BstDSI CCRYGG 1 cut(s) 57
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 6 cut(s) 75, 111, 228, 359, 541, 603
BstKTI GATC 5 cut(s) 21, 193, 430, 450, 640
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 5 cut(s) 18, 190, 427, 447, 637
BstMWI GCNNNNNNNGC 3 cut(s) 117, 249, 323
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 2 cut(s) 277, 406
BstPAI GACNNNNGTC 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 36, 156
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 2 cut(s) 18, 637
BstYI RGATCY 2 cut(s) 18, 637
BsuRI GGCC 2 cut(s) 243, 710
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 6 cut(s) 75, 111, 228, 359, 541, 603
BtsIMutI CAGTG 1 cut(s) 599
Cac8I GCNNGC 1 cut(s) 870
CaiI CAGNNNCTG 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 515, 740
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 549
CviAII CATG 6 cut(s) 58, 274, 403, 431, 683, 815
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 2 cut(s) 126, 437
DpnI GATC 5 cut(s) 20, 192, 429, 449, 639
DpnII GATC 5 cut(s) 18, 190, 427, 447, 637
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 3 cut(s) 462, 830, 858
EarI CTCTTC 3 cut(s) 462, 830, 858
EciI GGCGGA 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 57, 319
Eco47I GGWCC 2 cut(s) 515, 740
Eco57I CTGAAG 2 cut(s) 854, 912
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 2 cut(s) 57, 319
ErhI CCWWGG 2 cut(s) 57, 319
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 6 cut(s) 61, 277, 406, 434, 686, 818
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FatI CATG 6 cut(s) 57, 273, 402, 430, 682, 814
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 447
FblI GTMKAC 2 cut(s) 147, 382
Fnu4HI GCNGC 3 cut(s) 50, 170, 935
FokI GGATG 6 cut(s) 62, 98, 215, 346, 548, 610
Fsp4HI GCNGC 3 cut(s) 50, 170, 935
GluI GCNGC 3 cut(s) 50, 170, 935
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 2 cut(s) 243, 710
HgaI GACGC 1 cut(s) 109
Hin1II CATG 6 cut(s) 61, 277, 406, 434, 686, 818
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 4 cut(s) 98, 149, 197, 757
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 3 cut(s) 73, 148, 383
Hpy188I TCNGA 4 cut(s) 116, 526, 658, 892
Hpy188III TCNNGA 2 cut(s) 194, 445
Hpy8I GTNNAC 3 cut(s) 73, 148, 383
HpyAV CCTTC 3 cut(s) 9, 318, 887
HpyCH4III ACNGT 4 cut(s) 380, 387, 494, 594
HpyCH4V TGCA 7 cut(s) 95, 172, 235, 355, 411, 434, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 249, 323
HpyF3I CTNAG 2 cut(s) 126, 437
Hsp92II CATG 6 cut(s) 61, 277, 406, 434, 686, 818
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 5 cut(s) 18, 190, 427, 447, 637
LguI GCTCTTC 2 cut(s) 830, 858
LmnI GCTCC 7 cut(s) 46, 220, 331, 423, 483, 679, 819
Lsp1109I GCAGC 2 cut(s) 36, 156
LweI GCATC 3 cut(s) 107, 120, 420
MaeIII GTNAC 4 cut(s) 205, 398, 488, 748
MalI GATC 5 cut(s) 20, 192, 429, 449, 639
MbiI CCGCTC 1 cut(s) 215
MboI GATC 5 cut(s) 18, 190, 427, 447, 637
MboII GAAGA 6 cut(s) 16, 449, 803, 847, 875, 922
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 2 cut(s) 18, 637
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 9 cut(s) 37, 224, 507, 632, 644, 774, 784, 847, 913
MlyI GAGTC 3 cut(s) 107, 143, 751
MmeI TCCRAC 1 cut(s) 582
MroXI GAANNNNTTC 1 cut(s) 914
MseI TTAA 5 cut(s) 137, 165, 336, 585, 648
MspR9I CCNGG 1 cut(s) 68
MunI CAATTG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 3 cut(s) 117, 249, 323
NcoI CCATGG 1 cut(s) 57
NdeII GATC 5 cut(s) 18, 190, 427, 447, 637
NlaIII CATG 6 cut(s) 61, 277, 406, 434, 686, 818
NlaIV GGNNCC 1 cut(s) 20
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 2 cut(s) 277, 406
PciI ACATGT 1 cut(s) 402
PciSI GCTCTTC 2 cut(s) 830, 858
PcsI WCGNNNNNNNCGW 1 cut(s) 548
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 3 cut(s) 51, 171, 936
PleI GAGTC 3 cut(s) 106, 143, 751
PpsI GAGTC 3 cut(s) 106, 143, 751
PpuMI RGGWCCY 1 cut(s) 740
PscI ACATGT 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 2 cut(s) 515, 740
PspPPI RGGWCCY 1 cut(s) 740
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 2 cut(s) 18, 637
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SalI GTCGAC 1 cut(s) 146
SapI GCTCTTC 2 cut(s) 830, 858
SaqAI TTAA 5 cut(s) 137, 165, 336, 585, 648
SatI GCNGC 3 cut(s) 50, 170, 935
Sau3AI GATC 5 cut(s) 18, 190, 427, 447, 637
Sau96I GGNCC 2 cut(s) 515, 740
SchI GAGTC 3 cut(s) 107, 143, 751
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 3 cut(s) 107, 120, 420
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 2 cut(s) 515, 740
Sse9I AATT 9 cut(s) 37, 224, 507, 632, 644, 774, 784, 847, 913
SsiI CCGC 3 cut(s) 22, 213, 934
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 2 cut(s) 57, 319
TaaI ACNGT 4 cut(s) 380, 387, 494, 594
TaqI TCGA 3 cut(s) 147, 460, 542
TasI AATT 9 cut(s) 37, 224, 507, 632, 644, 774, 784, 847, 913
TauI GCSGC 1 cut(s) 937
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 5 cut(s) 137, 165, 336, 585, 648
Tru9I TTAA 5 cut(s) 137, 165, 336, 585, 648
TscAI CASTG 1 cut(s) 599
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 2 cut(s) 49, 169
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 6 cut(s) 17, 217, 278, 587, 693, 803
TspRI CASTG 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 515, 740
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 4 cut(s) 37, 507, 847, 913
XceI RCATGY 2 cut(s) 277, 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 2 cut(s) 147, 382
XmnI GAANNNNTTC 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.