Rmu_sc0020812.1_g000004

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020812.1
OG0000125
Physical Location & Seq
Reverse (-)
17248 .. 18531
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020812.1_g000004.1.cds

Sequence Viewer

Length: 1284 bp
atgatgaccccgagggtcctcaagagatcgaggtcttacagcagcagcaacacaaagcttctggacctccccagagatatccttgtcgatatggttctgagactgcccctgaaatcagtttcttccatccgatgtgcctctaagaggttgaacaacatagtcgacaaaatttttgttgcacagtacactcggcgtctacacaatactcttcctactggtgaagtacctcaacttatgcttcttgctagatcacacgagggaatcaaaacagtaacgaagaccttccacccacttcaatatcacggtggcaacgccttgaatatgaaagcacatggaaatggctcagagatcatagtaagccagaaatgtacctacgaggtaggttgtgttttcggcaacttgatttgctttaaagatatcagttatggaggtccgagcttcttgttgaatcctctaatgggagaagttctacggcttccggctagtaaggtgccaatcccagcctctattagatgtcgccgtgagctttttgactggcttgctatgggatttgatgacacaaccggcacgcacaagattcttcgtgtttgcggtaattcacagtacaagaattacctggtggcccaagtacttgtattgggtactgattcatggcgaaagatacgtgcagttcctccttgtaatttgagccacaaaaatgcgtctgcatgtggagacatgcattggatgattgacaaacaatcgagaactagaggaaggggagaaagtaatatagtttccttcgacttcaagaaagaggagtttcattggacttctcatcccaaattaagaagtcagttttccgacttgaatttggttaatctgaaaggatgcttagcgattgtggatgcttggttacatacccatcgcttgtccatcgagatatgggtgttgaaaaattatgaagtgaaagagtgggtgcgagagtaccacacagaaatcaaatttttgtttccttgttttacgggtagttttggtgaatgggaacatggtatatttttcaaagatgcaattagctgggggtttcagaacagagctactgcaatgtttttggatctaagaggtagtagtgttcaagtggaactggtgaagtgtcctataacattaatgcagaactggactatcatcagttatactgggagcctaatttccctaaaacattacggcaatttggttgaagcggaagcatgtgatgagtaccggaagcagcttggtttctctgaaaaattatggctaattgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

427

Amino Acids

48.82

Weight (kDa)

9.26

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48550 AT5G48550
fragaria_vesca FvH4_1g00551 FvH4_1g00570 FvH4_1g00691 FvH4_1g00700 FvH4_1g00812 FvH4_1g20161 FvH4_1g25190 FvH4_2g04190 FvH4_2g20921 FvH4_2g25871 FvH4_3g13181 FvH4_3g19662 FvH4_3g40671 FvH4_4g22410 FvH4_4g34520 FvH4_4g34580 FvH4_5g06780 FvH4_5g07350 FvH4_5g07470 FvH4_5g07510 FvH4_5g08848 FvH4_5g31171 FvH4_5g38320 FvH4_6g03511 FvH4_6g09071 FvH4_6g09550 FvH4_6g09801 FvH4_6g09911 FvH4_6g14781 FvH4_6g22690 FvH4_6g45801 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47471 FvH4_6g47480
malus_domestica MD04G1179800.v1.1 MD11G1005600.v1.1 MD11G1006200.v1.1 MD11G1006300.v1.1 MD11G1006900.v1.1 MD12G1195000.v1.1 MD12G1195500.v1.1 MD12G1195600.v1.1
prunus_persica Prupe.2G095600_v2.0.a1 Prupe.2G095700_v2.0.a1 Prupe.2G095800_v2.0.a1 Prupe.2G095900_v2.0.a1 Prupe.2G096000_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G209200_v2.0.a1 Prupe.3G238400_v2.0.a1 Prupe.7G182200_v2.0.a1 Prupe.7G194900_v2.0.a1 Prupe.7G195100_v2.0.a1 Prupe.7G195400_v2.0.a1 Prupe.8G061400_v2.0.a1
pyrus_communis pycom01g13020 pycom04g15890 pycom09g19490 pycom09g19510 pycom10g28160 pycom12g18120 pycom12g18180
rosa_chinensis RchiOBHm_Chr2g0085201 RchiOBHm_Chr2g0164001 RchiOBHm_Chr3g0449831 RchiOBHm_Chr3g0460411 RchiOBHm_Chr3g0480281 RchiOBHm_Chr4g0428611 RchiOBHm_Chr5g0016491 RchiOBHm_Chr6g0253541
rosa_laevigata RLG00000004425 RLG00000004426 RLG00000005189 RLG00000005350 RLG00000007140 RLG00000015004 RLG00000015666 RLG00000015801 RLG00000015804 RLG00000015807 RLG00000018680 RLG00000021432 RLG00000021625 RLG00000021626 RLG00000023498 RLG00000023500 RLG00000023502 RLG00000023505 RLG00000025014 RLG00000025039 RLG00000025070 RLG00000025093 RLG00000025094 RLG00000025791 RLG00000026900 RLG00000031177
rosa_multiflora Rmu_co8452909.1_g000001 Rmu_co8488687.1_g000001 Rmu_sc0000610.1_g000006 Rmu_sc0000770.1_g000026 Rmu_sc0001755.1_g000003 Rmu_sc0002072.1_g000002 Rmu_sc0002676.1_g000002 Rmu_sc0003133.1_g000022 Rmu_sc0003797.1_g000014 Rmu_sc0003901.1_g000002 Rmu_sc0003901.1_g000004 Rmu_sc0003906.1_g000004 Rmu_sc0004212.1_g000002 Rmu_sc0004621.1_g000026 Rmu_sc0006704.1_g000010 Rmu_sc0006944.1_g000006 Rmu_sc0008647.1_g000003 Rmu_sc0010698.1_g000005 Rmu_sc0010842.1_g000001 Rmu_sc0010911.1_g000004 Rmu_sc0011989.1_g000005 Rmu_sc0013983.1_g000002 Rmu_sc0016910.1_g000001 Rmu_sc0017276.1_g000001 Rmu_sc0020812.1_g000004 Rmu_sc0024877.1_g000001 Rmu_sc0025932.1_g000003 Rmu_ssc0000432.1_g000018
rosa_roxburghii Rroxscaffold_1G00072180 Rroxscaffold_1G00073060 Rroxscaffold_1G00074760 Rroxscaffold_2G00084790 Rroxscaffold_2G00086850 Rroxscaffold_2G00121570 Rroxscaffold_2G00154120 Rroxscaffold_3G00263530 Rroxscaffold_3G00273960 Rroxscaffold_4G00284960 Rroxscaffold_4G00284970 Rroxscaffold_5G00370300 Rroxscaffold_6G00401620 Rroxscaffold_6G00401630 Rroxscaffold_6G00401640 Rroxscaffold_6G00416730 Rroxscaffold_6G00419520 Rroxscaffold_6G00419780 Rroxscaffold_6G00420290 Rroxscaffold_6G00427650 Rroxscaffold_7G00176150 Rroxscaffold_7G00211790
rosa_rugosa Rorug02G0239000
rosa_samantha Rh1DG375000 Rh2AG006800 Rh2AG009900 Rh2AG024700 Rh2AG297700 Rh2AG574100 Rh2AG589700 Rh2AG589800 Rh2DG008500 Rh2DG012500 Rh2DG025200 Rh2DG321300 Rh2DG594200 Rh2DG612500 Rh3AG021800 Rh3AG093100 Rh3AG095100 Rh3AG095700 Rh3AG233400 Rh3AG233500 Rh3AG233600 Rh3CG021300 Rh3CG097200 Rh3CG097300 Rh3CG099400 Rh3CG100200 Rh3CG103500 Rh3CG103600 Rh3CG106200 Rh3CG263600 Rh3CG263700 Rh4DG287300 Rh5AG034300 Rh5AG495600 Rh5DG032900 Rh5DG033000 Rh5DG051400 Rh5DG532200 Rh6BG054100 Rh6BG337400 Rh6CG053800 Rh6CG343700 Rh6CG343800 Rh7AG043600 Rh7AG043800 Rh7AG121200 Rh7AG121300 Rh7DG025000 Rh7DG043300 Rh7DG124500 Rh7DG124600
rosa_wichuraiana Rw1G033430 Rw2G000640 Rw2G001940 Rw2G001990 Rw2G023800 Rw2G047500 Rw2G049160 Rw3G008010 Rw3G008540 Rw3G021000 Rw4G024660 Rw5G003140 Rw6G005370 Rw7G041040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 2 cut(s) 162, 196
AciI CCGC 2 cut(s) 591, 1220
AclWI GGATC 1 cut(s) 1101
AcsI RAATTY 3 cut(s) 168, 850, 983
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 8 cut(s) 185, 225, 370, 605, 630, 643, 968, 1238
AfiI CCNNNNNNNGG 2 cut(s) 144, 458
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 6 cut(s) 58, 438, 526, 1056, 1076, 1249
AluI AGCT 6 cut(s) 58, 438, 526, 1056, 1076, 1249
Alw26I GTCTC 2 cut(s) 94, 708
AlwI GGATC 1 cut(s) 1101
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 621
ApeKI GCWGC 3 cut(s) 42, 45, 1246
ApoI RAATTY 3 cut(s) 168, 850, 983
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 1 cut(s) 1145
Asp700I GAANNNNTTC 1 cut(s) 281
AspS9I GGNCC 4 cut(s) 16, 64, 431, 622
AsuHPI GGTGA 3 cut(s) 230, 1028, 1138
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 3 cut(s) 16, 64, 431
BanI GGYRCC 1 cut(s) 490
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 54, 57, 1258
BccI CCATC 3 cut(s) 134, 912, 923
BceAI ACGGC 3 cut(s) 488, 504, 1219
BciT130I CCWGG 1 cut(s) 617
BcoDI GTCTC 2 cut(s) 94, 708
BfaI CTAG 3 cut(s) 246, 483, 750
BisI GCNGC 3 cut(s) 43, 46, 1247
BlpI GCTNAGC 1 cut(s) 874
BlsI GCNGC 3 cut(s) 44, 47, 1248
BmcAI AGTACT 1 cut(s) 630
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 3 cut(s) 16, 64, 431
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 16, 64, 431, 622
BmiI GGNNCC 3 cut(s) 17, 492, 1181
BmrFI CCNGG 1 cut(s) 617
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 3 cut(s) 860, 877, 1036
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 874
BpuEI CTTGAG 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 458
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 5 cut(s) 220, 539, 1128, 1160, 1180
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 5 cut(s) 126, 732, 817, 875, 892
BseLI CCNNNNNNNGG 2 cut(s) 144, 458
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 2 cut(s) 89, 357
BseNI ACTGG 5 cut(s) 220, 539, 1128, 1160, 1180
BseRI GAGGAG 1 cut(s) 812
BseXI GCAGC 3 cut(s) 54, 57, 1258
BseYI CCCAGC 2 cut(s) 499, 1056
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 1 cut(s) 623
BshNI GGYRCC 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 3 cut(s) 479, 564, 1240
BslI CCNNNNNNNGG 2 cut(s) 144, 458
BsmAI GTCTC 2 cut(s) 94, 708
BsnI GGCC 1 cut(s) 623
BsoBI CYCGRG 1 cut(s) 10
Bsp143I GATC 4 cut(s) 26, 248, 348, 1093
Bsp1720I GCTNAGC 1 cut(s) 874
BspACI CCGC 2 cut(s) 591, 1220
BspANI GGCC 1 cut(s) 623
BspCNI CTCAG 2 cut(s) 90, 356
BspLI GGNNCC 3 cut(s) 17, 492, 1181
BspPI GGATC 1 cut(s) 1101
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 1089
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 5 cut(s) 220, 539, 1128, 1160, 1180
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 4 cut(s) 26, 248, 348, 1093
BssNI GRCGYC 1 cut(s) 193
BssSI CACGAG 1 cut(s) 254
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 4 cut(s) 183, 271, 305, 603
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 540, 569
BstDEI CTNAG 5 cut(s) 98, 141, 343, 874, 1097
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 5 cut(s) 126, 732, 817, 875, 892
BstKTI GATC 4 cut(s) 29, 251, 351, 1096
BstMAI GTCTC 2 cut(s) 94, 708
BstMBI GATC 4 cut(s) 26, 248, 348, 1093
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 3 cut(s) 711, 721, 1230
BstSCI CCNGG 1 cut(s) 615
BstV1I GCAGC 3 cut(s) 54, 57, 1258
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 1093
BsuRI GGCC 1 cut(s) 623
BtgZI GCGATG 1 cut(s) 890
BtsCI GGATG 5 cut(s) 126, 732, 817, 875, 892
Cac8I GCNNGC 2 cut(s) 540, 569
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 4 cut(s) 16, 64, 431, 622
CseI GACGC 2 cut(s) 182, 690
CsiI ACCWGGT 1 cut(s) 615
Csp6I GTAC 8 cut(s) 184, 224, 369, 604, 629, 642, 967, 1237
CviAII CATG 6 cut(s) 332, 651, 708, 718, 1028, 1227
CviQI GTAC 8 cut(s) 184, 224, 369, 604, 629, 642, 967, 1237
DdeI CTNAG 5 cut(s) 98, 141, 343, 874, 1097
DpnI GATC 4 cut(s) 28, 250, 350, 1095
DpnII GATC 4 cut(s) 26, 248, 348, 1093
DraI TTTAAA 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco32I GATATC 2 cut(s) 79, 418
Eco47I GGWCC 3 cut(s) 16, 64, 431
Eco88I CYCGRG 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 615
EcoRV GATATC 2 cut(s) 79, 418
EcoT22I ATGCAT 1 cut(s) 723
FaeI CATG 6 cut(s) 335, 654, 711, 721, 1031, 1230
FatI CATG 6 cut(s) 331, 650, 707, 717, 1027, 1226
FblI GTMKAC 2 cut(s) 162, 196
Fnu4HI GCNGC 3 cut(s) 43, 46, 1247
FokI GGATG 5 cut(s) 113, 739, 804, 882, 899
Fsp4HI GCNGC 3 cut(s) 43, 46, 1247
FspBI CTAG 3 cut(s) 246, 483, 750
GluI GCNGC 3 cut(s) 43, 46, 1247
GsaI CCCAGC 2 cut(s) 503, 1060
HaeIII GGCC 1 cut(s) 623
HapII CCGG 3 cut(s) 479, 564, 1240
HgaI GACGC 2 cut(s) 182, 690
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 6 cut(s) 335, 654, 711, 721, 1031, 1230
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 261, 448, 577, 647
HpaII CCGG 3 cut(s) 479, 564, 1240
HphI GGTGA 3 cut(s) 230, 1028, 1138
Hpy166II GTNNAC 3 cut(s) 163, 186, 197
Hpy188I TCNGA 8 cut(s) 99, 131, 346, 435, 844, 864, 1068, 1261
Hpy188III TCNNGA 5 cut(s) 22, 62, 744, 790, 919
Hpy8I GTNNAC 3 cut(s) 163, 186, 197
HpyAV CCTTC 3 cut(s) 292, 750, 790
HpyCH4III ACNGT 4 cut(s) 183, 271, 305, 603
HpyCH4IV ACGT 1 cut(s) 664
HpyCH4V TGCA 7 cut(s) 179, 668, 707, 721, 1049, 1082, 1150
HpyF3I CTNAG 5 cut(s) 98, 141, 343, 874, 1097
HpySE526I ACGT 1 cut(s) 664
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 6 cut(s) 335, 654, 711, 721, 1031, 1230
Kzo9I GATC 4 cut(s) 26, 248, 348, 1093
LmnI GCTCC 1 cut(s) 1179
Lsp1109I GCAGC 3 cut(s) 54, 57, 1258
LweI GCATC 3 cut(s) 860, 877, 1036
MabI ACCWGGT 1 cut(s) 615
MaeI CTAG 3 cut(s) 246, 483, 750
MaeII ACGT 1 cut(s) 664
MaeIII GTNAC 2 cut(s) 271, 894
MalI GATC 4 cut(s) 28, 250, 350, 1095
MboI GATC 4 cut(s) 26, 248, 348, 1093
MboII GAAGA 4 cut(s) 114, 200, 289, 572
MflI RGATCY 1 cut(s) 1093
MmeI TCCRAC 1 cut(s) 867
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 5 cut(s) 411, 827, 858, 1145, 1282
MslI CAYNNNNRTG 2 cut(s) 336, 696
MspI CCGG 3 cut(s) 479, 564, 1240
MspR9I CCNGG 1 cut(s) 617
MvaI CCWGG 1 cut(s) 617
NdeII GATC 4 cut(s) 26, 248, 348, 1093
NlaIII CATG 6 cut(s) 335, 654, 711, 721, 1031, 1230
NlaIV GGNNCC 3 cut(s) 17, 492, 1181
NmeAIII GCCGAG 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 723
NspI RCATGY 3 cut(s) 711, 721, 1230
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 4 cut(s) 261, 448, 577, 647
PkrI GCNGC 3 cut(s) 44, 47, 1248
Ppu21I YACGTR 1 cut(s) 665
PpuMI RGGWCCY 1 cut(s) 16
PshBI ATTAAT 1 cut(s) 1145
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 2 cut(s) 499, 1056
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 17, 492, 1181
PspPI GGNCC 4 cut(s) 16, 64, 431, 622
PspPPI RGGWCCY 1 cut(s) 16
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 8 cut(s) 185, 225, 370, 605, 630, 643, 968, 1238
RsaNI GTAC 8 cut(s) 184, 224, 369, 604, 629, 642, 967, 1237
RseI CAYNNNNRTG 2 cut(s) 336, 696
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 5 cut(s) 411, 827, 858, 1145, 1282
SatI GCNGC 3 cut(s) 43, 46, 1247
Sau3AI GATC 4 cut(s) 26, 248, 348, 1093
Sau96I GGNCC 4 cut(s) 16, 64, 431, 622
ScaI AGTACT 1 cut(s) 630
ScrFI CCNGG 1 cut(s) 617
SexAI ACCWGGT 1 cut(s) 615
SfaNI GCATC 3 cut(s) 860, 877, 1036
SinI GGWCC 3 cut(s) 16, 64, 431
SmiMI CAYNNNNRTG 2 cut(s) 336, 696
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SsiI CCGC 2 cut(s) 591, 1220
SspMI CTAG 3 cut(s) 246, 483, 750
StyD4I CCNGG 1 cut(s) 615
TaaI ACNGT 4 cut(s) 183, 271, 305, 603
TaiI ACGT 1 cut(s) 667
TaqI TCGA 6 cut(s) 29, 87, 162, 743, 783, 918
TatI WGTACW 3 cut(s) 183, 603, 628
TfiI GAWTC 4 cut(s) 261, 448, 577, 647
Tru1I TTAA 5 cut(s) 411, 827, 858, 1145, 1282
Tru9I TTAA 5 cut(s) 411, 827, 858, 1145, 1282
TseI GCWGC 3 cut(s) 42, 45, 1246
TspDTI ATGAA 4 cut(s) 338, 639, 794, 957
VpaK11BI GGWCC 3 cut(s) 16, 64, 431
VspI ATTAAT 1 cut(s) 1145
XagI CCTNNNNNAGG 1 cut(s) 142
XapI RAATTY 3 cut(s) 168, 850, 983
XceI RCATGY 3 cut(s) 711, 721, 1230
XmiI GTMKAC 2 cut(s) 162, 196
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 3 cut(s) 246, 483, 750
ZrmI AGTACT 1 cut(s) 630
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.