RLG00000025039

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000125
Physical Location & Seq
Forward (+)
41501590 .. 41502858
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025039

Sequence Viewer

Length: 960 bp
ATGGCGATGTGCAGCAACAACAGCAGGGAAAAGCTGATGGTGATGAATCAGGATACAAACTACCTTCCCCTGGATCTCTTCGTGAACATCCTTGTGAGATTGCCTGCTGAATCACTCTGTTGCATCCGATGCGTGTCCAAGGCCATGTTAGACATGGTTGATAGCCACTCTTTCGTTACGCTGCACACCCGGTCGCTGGTGACCTCTAGTGATCATGTGCCTCAACTTATGTATCTTACCAGATTCTTCAAACGTAAAAAATTTAATGCTTCTCGAGGTTATATGGAATTAGAAGCTCTCAAGTACGATGGCAACACCACCTTGACAATAGGAAAACGTGCACTTACCTTGTGTACATCAAAACCGTACGATCTTCACCAATTCAAGGTAGATTTCGTTTTCTACAACTTATTTTGCTTCAGAAATATACAAGAGACGTTATATGGACATTGCTTCTTGGTTGATCCTCTCCGTGGAGAAATTCTGGTGCTTCCACCTCGTGACATACCCGATACAGATTTCAAATTTTTCTGTGGTGATTACTATGGTATGGGATTTGATAGTTTAAATGTTACTCTACGAGGATCTCTGGCCACAGTGGATACTTGTTTATCAACTGCTGTGACTATGAATGTTGAGATGTGGGTATTGAAAGACTATGATAAGAAAATATGGACGAGAGACTACACCATAAGTATTGAAATGGTCGGATTGCGTCCAAATTTTTTGTCCACTGATATCATCTGTGGTGAATGGGAGCATGGCATATTTTTCAAGCATCTGGGCGAGAGCGGTAACATCTTTTTCTTGGATCTGAAGCGTCTTTCGATGAAGGTTGTAACATATGGGACTGGAAATAATAGTGACATCTATAGTTATACCATGAGCTTGATTTCCTTGAAAGATTATGAGAATTCGGTTAGAGTTGAAGCAAGCCGATTTGTTGAAGTGGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

320

Amino Acids

36.79

Weight (kDa)

6.18

Isoelectric Point (pI)

24.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 58 2.6e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48550 AT5G48550
fragaria_vesca FvH4_1g00551 FvH4_1g00570 FvH4_1g00691 FvH4_1g00700 FvH4_1g00812 FvH4_1g20161 FvH4_1g25190 FvH4_2g04190 FvH4_2g20921 FvH4_2g25871 FvH4_3g13181 FvH4_3g19662 FvH4_3g40671 FvH4_4g22410 FvH4_4g34520 FvH4_4g34580 FvH4_5g06780 FvH4_5g07350 FvH4_5g07470 FvH4_5g07510 FvH4_5g08848 FvH4_5g31171 FvH4_5g38320 FvH4_6g03511 FvH4_6g09071 FvH4_6g09550 FvH4_6g09801 FvH4_6g09911 FvH4_6g14781 FvH4_6g22690 FvH4_6g45801 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47471 FvH4_6g47480
malus_domestica MD04G1179800.v1.1 MD11G1005600.v1.1 MD11G1006200.v1.1 MD11G1006300.v1.1 MD11G1006900.v1.1 MD12G1195000.v1.1 MD12G1195500.v1.1 MD12G1195600.v1.1
prunus_persica Prupe.2G095600_v2.0.a1 Prupe.2G095700_v2.0.a1 Prupe.2G095800_v2.0.a1 Prupe.2G095900_v2.0.a1 Prupe.2G096000_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G209200_v2.0.a1 Prupe.3G238400_v2.0.a1 Prupe.7G182200_v2.0.a1 Prupe.7G194900_v2.0.a1 Prupe.7G195100_v2.0.a1 Prupe.7G195400_v2.0.a1 Prupe.8G061400_v2.0.a1
pyrus_communis pycom01g13020 pycom04g15890 pycom09g19490 pycom09g19510 pycom10g28160 pycom12g18120 pycom12g18180
rosa_chinensis RchiOBHm_Chr2g0085201 RchiOBHm_Chr2g0164001 RchiOBHm_Chr3g0449831 RchiOBHm_Chr3g0460411 RchiOBHm_Chr3g0480281 RchiOBHm_Chr4g0428611 RchiOBHm_Chr5g0016491 RchiOBHm_Chr6g0253541
rosa_laevigata RLG00000004425 RLG00000004426 RLG00000005189 RLG00000005350 RLG00000007140 RLG00000015004 RLG00000015666 RLG00000015801 RLG00000015804 RLG00000015807 RLG00000018680 RLG00000021432 RLG00000021625 RLG00000021626 RLG00000023498 RLG00000023500 RLG00000023502 RLG00000023505 RLG00000025014 RLG00000025039 RLG00000025070 RLG00000025093 RLG00000025094 RLG00000025791 RLG00000026900 RLG00000031177
rosa_multiflora Rmu_co8452909.1_g000001 Rmu_co8488687.1_g000001 Rmu_sc0000610.1_g000006 Rmu_sc0000770.1_g000026 Rmu_sc0001755.1_g000003 Rmu_sc0002072.1_g000002 Rmu_sc0002676.1_g000002 Rmu_sc0003133.1_g000022 Rmu_sc0003797.1_g000014 Rmu_sc0003901.1_g000002 Rmu_sc0003901.1_g000004 Rmu_sc0003906.1_g000004 Rmu_sc0004212.1_g000002 Rmu_sc0004621.1_g000026 Rmu_sc0006704.1_g000010 Rmu_sc0006944.1_g000006 Rmu_sc0008647.1_g000003 Rmu_sc0010698.1_g000005 Rmu_sc0010842.1_g000001 Rmu_sc0010911.1_g000004 Rmu_sc0011989.1_g000005 Rmu_sc0013983.1_g000002 Rmu_sc0016910.1_g000001 Rmu_sc0017276.1_g000001 Rmu_sc0020812.1_g000004 Rmu_sc0024877.1_g000001 Rmu_sc0025932.1_g000003 Rmu_ssc0000432.1_g000018
rosa_roxburghii Rroxscaffold_1G00072180 Rroxscaffold_1G00073060 Rroxscaffold_1G00074760 Rroxscaffold_2G00084790 Rroxscaffold_2G00086850 Rroxscaffold_2G00121570 Rroxscaffold_2G00154120 Rroxscaffold_3G00263530 Rroxscaffold_3G00273960 Rroxscaffold_4G00284960 Rroxscaffold_4G00284970 Rroxscaffold_5G00370300 Rroxscaffold_6G00401620 Rroxscaffold_6G00401630 Rroxscaffold_6G00401640 Rroxscaffold_6G00416730 Rroxscaffold_6G00419520 Rroxscaffold_6G00419780 Rroxscaffold_6G00420290 Rroxscaffold_6G00427650 Rroxscaffold_7G00176150 Rroxscaffold_7G00211790
rosa_rugosa Rorug02G0239000
rosa_samantha Rh1DG375000 Rh2AG006800 Rh2AG009900 Rh2AG024700 Rh2AG297700 Rh2AG574100 Rh2AG589700 Rh2AG589800 Rh2DG008500 Rh2DG012500 Rh2DG025200 Rh2DG321300 Rh2DG594200 Rh2DG612500 Rh3AG021800 Rh3AG093100 Rh3AG095100 Rh3AG095700 Rh3AG233400 Rh3AG233500 Rh3AG233600 Rh3CG021300 Rh3CG097200 Rh3CG097300 Rh3CG099400 Rh3CG100200 Rh3CG103500 Rh3CG103600 Rh3CG106200 Rh3CG263600 Rh3CG263700 Rh4DG287300 Rh5AG034300 Rh5AG495600 Rh5DG032900 Rh5DG033000 Rh5DG051400 Rh5DG532200 Rh6BG054100 Rh6BG337400 Rh6CG053800 Rh6CG343700 Rh6CG343800 Rh7AG043600 Rh7AG043800 Rh7AG121200 Rh7AG121300 Rh7DG025000 Rh7DG043300 Rh7DG124500 Rh7DG124600
rosa_wichuraiana Rw1G033430 Rw2G000640 Rw2G001940 Rw2G001990 Rw2G023800 Rw2G047500 Rw2G049160 Rw3G008010 Rw3G008540 Rw3G021000 Rw4G024660 Rw5G003140 Rw6G005370 Rw7G041040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 792
AciI CCGC 1 cut(s) 792
AclWI GGATC 4 cut(s) 81, 458, 592, 819
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 5 cut(s) 260, 480, 524, 721, 913
AcuI CTGAAG 2 cut(s) 403, 836
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 3 cut(s) 305, 355, 368
AfiI CCNNNNNNNGG 4 cut(s) 70, 196, 385, 473
AgsI TTSAA 9 cut(s) 250, 385, 523, 652, 701, 775, 901, 929, 947
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 3 cut(s) 34, 296, 888
AluI AGCT 3 cut(s) 34, 296, 888
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 2 cut(s) 428, 675
Alw44I GTGCAC 1 cut(s) 339
AlwI GGATC 4 cut(s) 81, 458, 592, 819
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 2 cut(s) 141, 591
ApaLI GTGCAC 1 cut(s) 339
ApeKI GCWGC 2 cut(s) 12, 181
ApoI RAATTY 5 cut(s) 260, 480, 524, 721, 913
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 5 cut(s) 52, 211, 368, 548, 761
AvaI CYCGRG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 343
BaeI ACNNNNGTAYC 2 cut(s) 215, 248
BalI TGGCCA 1 cut(s) 593
BauI CACGAG 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 2 cut(s) 24, 168
BccI CCATC 2 cut(s) 31, 302
BciT130I CCWGG 1 cut(s) 71
BciVI GTATCC 2 cut(s) 46, 595
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 428, 675
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 871
BfuI GTATCC 2 cut(s) 46, 595
BisI GCNGC 2 cut(s) 13, 182
BlsI GCNGC 2 cut(s) 14, 183
Bme1390I CCNGG 2 cut(s) 71, 190
BmeT110I CYCGRG 1 cut(s) 273
BmrFI CCNGG 2 cut(s) 71, 190
BmsI GCATC 3 cut(s) 119, 132, 787
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 190
BsaJI CCNNGG 3 cut(s) 69, 138, 472
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 196, 385, 473
Bse1I ACTGG 1 cut(s) 856
Bse3DI GCAATG 1 cut(s) 448
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 69, 138, 472
BseGI GGATG 2 cut(s) 87, 123
BseLI CCNNNNNNNGG 4 cut(s) 70, 196, 385, 473
BseMI GCAATG 1 cut(s) 448
BseNI ACTGG 1 cut(s) 856
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 2 cut(s) 24, 168
BsgI GTGCAG 2 cut(s) 31, 167
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 2 cut(s) 143, 593
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 343
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 190
BsiWI CGTACG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 4 cut(s) 70, 196, 385, 473
BsmAI GTCTC 2 cut(s) 428, 675
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 2 cut(s) 143, 593
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 353
Bsp143I GATC 6 cut(s) 73, 211, 370, 463, 584, 811
BspACI CCGC 1 cut(s) 792
BspANI GGCC 2 cut(s) 143, 593
BspPI GGATC 4 cut(s) 81, 458, 592, 819
BsrBI CCGCTC 1 cut(s) 792
BsrDI GCAATG 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 353
BsrI ACTGG 1 cut(s) 856
BssECI CCNNGG 3 cut(s) 69, 138, 472
BssMI GATC 6 cut(s) 73, 211, 370, 463, 584, 811
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 366, 598
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 105, 934
BstDSI CCRYGG 1 cut(s) 472
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 2 cut(s) 87, 123
BstKTI GATC 6 cut(s) 76, 214, 373, 466, 587, 814
BstMAI GTCTC 2 cut(s) 428, 675
BstMBI GATC 6 cut(s) 73, 211, 370, 463, 584, 811
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 2 cut(s) 21, 129
BstNI CCWGG 1 cut(s) 71
BstPI GGTNACC 1 cut(s) 199
BstSCI CCNGG 2 cut(s) 69, 188
BstSFI CTRYAG 1 cut(s) 871
BstSLI GKGCMC 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 24, 168
BstX2I RGATCY 3 cut(s) 73, 584, 811
BstYI RGATCY 3 cut(s) 73, 584, 811
BsuI GTATCC 2 cut(s) 46, 595
BsuRI GGCC 2 cut(s) 143, 593
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 87, 123
BtsIMutI CAGTG 2 cut(s) 603, 732
Cac8I GCNNGC 2 cut(s) 105, 934
CseI GACGC 2 cut(s) 704, 809
Csp6I GTAC 3 cut(s) 304, 354, 367
CviAII CATG 5 cut(s) 145, 154, 215, 761, 883
CviJI RGCY 7 cut(s) 34, 143, 165, 296, 593, 888, 936
CviKI_1 RGCY 7 cut(s) 34, 143, 165, 296, 593, 888, 936
CviQI GTAC 3 cut(s) 304, 354, 367
DpnI GATC 6 cut(s) 75, 213, 372, 465, 586, 813
DpnII GATC 6 cut(s) 73, 211, 370, 463, 584, 811
DraI TTTAAA 1 cut(s) 567
DraIII CACNNNGTG 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 138
Eco32I GATATC 1 cut(s) 739
Eco57I CTGAAG 2 cut(s) 403, 836
Eco88I CYCGRG 1 cut(s) 273
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 1 cut(s) 69
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 5 cut(s) 148, 157, 218, 764, 886
FaqI GGGAC 1 cut(s) 862
FatI CATG 5 cut(s) 144, 153, 214, 760, 882
FauNDI CATATG 1 cut(s) 844
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 13, 182
FokI GGATG 2 cut(s) 74, 110
Fsp4HI GCNGC 2 cut(s) 13, 182
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 13, 182
HaeIII GGCC 2 cut(s) 143, 593
HapII CCGG 1 cut(s) 190
HgaI GACGC 2 cut(s) 704, 809
Hin1II CATG 5 cut(s) 148, 157, 218, 764, 886
HinfI GANTC 3 cut(s) 46, 110, 243
HpaII CCGG 1 cut(s) 190
HphI GGTGA 5 cut(s) 52, 211, 368, 548, 761
Hpy166II GTNNAC 4 cut(s) 85, 341, 354, 732
Hpy188I TCNGA 4 cut(s) 128, 422, 710, 816
Hpy188III TCNNGA 4 cut(s) 50, 82, 273, 500
Hpy8I GTNNAC 4 cut(s) 85, 341, 354, 732
HpyAV CCTTC 2 cut(s) 74, 826
HpyCH4III ACNGT 2 cut(s) 366, 598
HpyCH4IV ACGT 3 cut(s) 253, 337, 437
HpyCH4V TGCA 4 cut(s) 12, 123, 184, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 129
HpySE526I ACGT 3 cut(s) 253, 337, 437
Hsp92II CATG 5 cut(s) 148, 157, 218, 764, 886
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 6 cut(s) 73, 211, 370, 463, 584, 811
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 2 cut(s) 24, 168
LweI GCATC 3 cut(s) 119, 132, 787
MaeI CTAG 1 cut(s) 207
MaeII ACGT 3 cut(s) 253, 337, 437
MaeIII GTNAC 8 cut(s) 175, 199, 500, 571, 622, 794, 838, 863
MalI GATC 6 cut(s) 75, 213, 372, 465, 586, 813
MbiI CCGCTC 1 cut(s) 792
MboI GATC 6 cut(s) 73, 211, 370, 463, 584, 811
MboII GAAGA 3 cut(s) 70, 238, 365
MflI RGATCY 3 cut(s) 73, 584, 811
MhlI GDGCHC 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 7 cut(s) 260, 287, 380, 480, 524, 721, 913
MluNI TGGCCA 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 6 cut(s) 214, 231, 269, 477, 507, 575
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MseI TTAA 2 cut(s) 264, 566
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 593
MspI CCGG 1 cut(s) 190
MspR9I CCNGG 2 cut(s) 71, 190
MvaI CCWGG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 21, 129
NciI CCSGG 1 cut(s) 190
NdeI CATATG 1 cut(s) 844
NdeII GATC 6 cut(s) 73, 211, 370, 463, 584, 811
NlaIII CATG 5 cut(s) 148, 157, 218, 764, 886
NmuCI GTSAC 4 cut(s) 199, 500, 622, 863
PaeR7I CTCGAG 1 cut(s) 273
PfeI GAWTC 3 cut(s) 46, 110, 243
Pfl23II CGTACG 1 cut(s) 366
PkrI GCNGC 2 cut(s) 14, 183
Psp6I CCWGG 1 cut(s) 69
PspEI GGTNACC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 69
PspLI CGTACG 1 cut(s) 366
PsuI RGATCY 3 cut(s) 73, 584, 811
RsaI GTAC 3 cut(s) 305, 355, 368
RsaNI GTAC 3 cut(s) 304, 354, 367
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 2 cut(s) 264, 566
SatI GCNGC 2 cut(s) 13, 182
Sau3AI GATC 6 cut(s) 73, 211, 370, 463, 584, 811
ScrFI CCNGG 2 cut(s) 71, 190
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 3 cut(s) 119, 132, 787
SfcI CTRYAG 1 cut(s) 871
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 273, 299
SmoI CTYRAG 2 cut(s) 273, 299
Sse9I AATT 7 cut(s) 260, 287, 380, 480, 524, 721, 913
SsiI CCGC 1 cut(s) 792
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 69, 188
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 366, 598
TaiI ACGT 3 cut(s) 256, 340, 440
TaqI TCGA 2 cut(s) 274, 827
TasI AATT 7 cut(s) 260, 287, 380, 480, 524, 721, 913
TatI WGTACW 1 cut(s) 353
TfiI GAWTC 3 cut(s) 46, 110, 243
Tru1I TTAA 2 cut(s) 264, 566
Tru9I TTAA 2 cut(s) 264, 566
TscAI CASTG 2 cut(s) 603, 739
TseFI GTSAC 4 cut(s) 199, 500, 622, 863
TseI GCWGC 2 cut(s) 12, 181
Tsp45I GTSAC 4 cut(s) 199, 500, 622, 863
TspDTI ATGAA 3 cut(s) 59, 644, 845
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 2 cut(s) 603, 739
VneI GTGCAC 1 cut(s) 339
XapI RAATTY 5 cut(s) 260, 480, 524, 721, 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 273
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.