pycom12g18180

F-box kelch-repeat protein At3g06240-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000125
Physical Location & Seq
Forward (+)
20400255 .. 20401408
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18180.2

Sequence Viewer

Length: 939 bp
ATGAAGAACATAGTTGACGGCCAGTCTATCGTTACACTTCACACGCGGTTACTTACTGCTACCAATGCTGTTGCAGAAGTACCTCAACTTATACTTATTGATAACATCGGCATGGCTTCTTTAGCGGCAGTACAATTATTCGAATACGATGGCAACTCCTCCTTGAAAGAGAGAAAACATGCAATTGTCTCAGAGATTGGATTCGACAGTTTCAACGCTCGGATCGTTCAACAATTTGTTTTCTGCAACTTGTTTTGCTTTACAGTTGGGAACTTCTGCATCTTATTTGATCCTTTTAAGGGACAAGTTTTACCACTCCCGATGAGTGACGTCCAAGTTCGCGTAGAACACCGTTATGATGTGTACGAGAGCCGTATAACTTCTTTCGACTTCAAGAAAGAGGAGTTCTATTGCACTCCCCATCCTATTCAAACAAGGTGGAATATGCATCTATTTATTCTGAGAGGCTATATGGCGTTTTTGGCCGTCACAGGTACGCATGTTGAGATATGGGCGCCAAAAAATTATGAAAAAAAAGAGTGCACGCTTGAATACGGCATAGATCTTCGGGTTCATGGTCATACGCTCAGGGATTATTCGTGGATTGCTGGTTGTGGTCAGTGGGAGCACGGCATTTTTTTCTTGGATTCTGACGGCAGTGCATTGTTTTTCGACGTAAGATATCATTCCATAAAACATGCGACATATCCAATGTTATTTCCGAGAGACCCGGTGGTGGCGCGTAACCCTAGTATTCACAGTTGTACTCGGAGCTTGATTGCCCTCGAACACTATGGCAATGTGATTGACCAAGAACACATCGTGTCCGGTATTCGGAATCTGGCAAGTTTGAATGGACCTTCAAGAAAAATGTTATCCAACCTGAAGAACATTACTGGAAAGGTAAAGCTTTCAAACGGATTGTGGAAACACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

313

Amino Acids

35.63

Weight (kDa)

7.7

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48550 AT5G48550
fragaria_vesca FvH4_1g00551 FvH4_1g00570 FvH4_1g00691 FvH4_1g00700 FvH4_1g00812 FvH4_1g20161 FvH4_1g25190 FvH4_2g04190 FvH4_2g20921 FvH4_2g25871 FvH4_3g13181 FvH4_3g19662 FvH4_3g40671 FvH4_4g22410 FvH4_4g34520 FvH4_4g34580 FvH4_5g06780 FvH4_5g07350 FvH4_5g07470 FvH4_5g07510 FvH4_5g08848 FvH4_5g31171 FvH4_5g38320 FvH4_6g03511 FvH4_6g09071 FvH4_6g09550 FvH4_6g09801 FvH4_6g09911 FvH4_6g14781 FvH4_6g22690 FvH4_6g45801 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47440 FvH4_6g47471 FvH4_6g47480
malus_domestica MD04G1179800.v1.1 MD11G1005600.v1.1 MD11G1006200.v1.1 MD11G1006300.v1.1 MD11G1006900.v1.1 MD12G1195000.v1.1 MD12G1195500.v1.1 MD12G1195600.v1.1
prunus_persica Prupe.2G095600_v2.0.a1 Prupe.2G095700_v2.0.a1 Prupe.2G095800_v2.0.a1 Prupe.2G095900_v2.0.a1 Prupe.2G096000_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G196700_v2.0.a1 Prupe.2G209200_v2.0.a1 Prupe.3G238400_v2.0.a1 Prupe.7G182200_v2.0.a1 Prupe.7G194900_v2.0.a1 Prupe.7G195100_v2.0.a1 Prupe.7G195400_v2.0.a1 Prupe.8G061400_v2.0.a1
pyrus_communis pycom01g13020 pycom04g15890 pycom09g19490 pycom09g19510 pycom10g28160 pycom12g18120 pycom12g18180
rosa_chinensis RchiOBHm_Chr2g0085201 RchiOBHm_Chr2g0164001 RchiOBHm_Chr3g0449831 RchiOBHm_Chr3g0460411 RchiOBHm_Chr3g0480281 RchiOBHm_Chr4g0428611 RchiOBHm_Chr5g0016491 RchiOBHm_Chr6g0253541
rosa_laevigata RLG00000004425 RLG00000004426 RLG00000005189 RLG00000005350 RLG00000007140 RLG00000015004 RLG00000015666 RLG00000015801 RLG00000015804 RLG00000015807 RLG00000018680 RLG00000021432 RLG00000021625 RLG00000021626 RLG00000023498 RLG00000023500 RLG00000023502 RLG00000023505 RLG00000025014 RLG00000025039 RLG00000025070 RLG00000025093 RLG00000025094 RLG00000025791 RLG00000026900 RLG00000031177
rosa_multiflora Rmu_co8452909.1_g000001 Rmu_co8488687.1_g000001 Rmu_sc0000610.1_g000006 Rmu_sc0000770.1_g000026 Rmu_sc0001755.1_g000003 Rmu_sc0002072.1_g000002 Rmu_sc0002676.1_g000002 Rmu_sc0003133.1_g000022 Rmu_sc0003797.1_g000014 Rmu_sc0003901.1_g000002 Rmu_sc0003901.1_g000004 Rmu_sc0003906.1_g000004 Rmu_sc0004212.1_g000002 Rmu_sc0004621.1_g000026 Rmu_sc0006704.1_g000010 Rmu_sc0006944.1_g000006 Rmu_sc0008647.1_g000003 Rmu_sc0010698.1_g000005 Rmu_sc0010842.1_g000001 Rmu_sc0010911.1_g000004 Rmu_sc0011989.1_g000005 Rmu_sc0013983.1_g000002 Rmu_sc0016910.1_g000001 Rmu_sc0017276.1_g000001 Rmu_sc0020812.1_g000004 Rmu_sc0024877.1_g000001 Rmu_sc0025932.1_g000003 Rmu_ssc0000432.1_g000018
rosa_roxburghii Rroxscaffold_1G00072180 Rroxscaffold_1G00073060 Rroxscaffold_1G00074760 Rroxscaffold_2G00084790 Rroxscaffold_2G00086850 Rroxscaffold_2G00121570 Rroxscaffold_2G00154120 Rroxscaffold_3G00263530 Rroxscaffold_3G00273960 Rroxscaffold_4G00284960 Rroxscaffold_4G00284970 Rroxscaffold_5G00370300 Rroxscaffold_6G00401620 Rroxscaffold_6G00401630 Rroxscaffold_6G00401640 Rroxscaffold_6G00416730 Rroxscaffold_6G00419520 Rroxscaffold_6G00419780 Rroxscaffold_6G00420290 Rroxscaffold_6G00427650 Rroxscaffold_7G00176150 Rroxscaffold_7G00211790
rosa_rugosa Rorug02G0239000
rosa_samantha Rh1DG375000 Rh2AG006800 Rh2AG009900 Rh2AG024700 Rh2AG297700 Rh2AG574100 Rh2AG589700 Rh2AG589800 Rh2DG008500 Rh2DG012500 Rh2DG025200 Rh2DG321300 Rh2DG594200 Rh2DG612500 Rh3AG021800 Rh3AG093100 Rh3AG095100 Rh3AG095700 Rh3AG233400 Rh3AG233500 Rh3AG233600 Rh3CG021300 Rh3CG097200 Rh3CG097300 Rh3CG099400 Rh3CG100200 Rh3CG103500 Rh3CG103600 Rh3CG106200 Rh3CG263600 Rh3CG263700 Rh4DG287300 Rh5AG034300 Rh5AG495600 Rh5DG032900 Rh5DG033000 Rh5DG051400 Rh5DG532200 Rh6BG054100 Rh6BG337400 Rh6CG053800 Rh6CG343700 Rh6CG343800 Rh7AG043600 Rh7AG043800 Rh7AG121200 Rh7AG121300 Rh7DG025000 Rh7DG043300 Rh7DG124500 Rh7DG124600
rosa_wichuraiana Rw1G033430 Rw2G000640 Rw2G001940 Rw2G001990 Rw2G023800 Rw2G047500 Rw2G049160 Rw3G008010 Rw3G008540 Rw3G021000 Rw4G024660 Rw5G003140 Rw6G005370 Rw7G041040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 514
AccII CGCG 3 cut(s) 46, 342, 742
AciI CCGC 2 cut(s) 46, 125
AclWI GGATC 2 cut(s) 230, 284
AcoI YGGCCR 2 cut(s) 19, 483
AcuI CTGAAG 1 cut(s) 905
AcyI GRCGYC 2 cut(s) 330, 515
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 5 cut(s) 81, 132, 365, 496, 766
AfiI CCNNNNNNNGG 3 cut(s) 299, 736, 834
AgsI TTSAA 9 cut(s) 166, 214, 230, 394, 431, 551, 853, 864, 915
AhdI GACNNNNNGTC 1 cut(s) 22
AluBI AGCT 2 cut(s) 774, 910
AluI AGCT 2 cut(s) 774, 910
Alw21I GWGCWC 2 cut(s) 545, 630
Alw26I GTCTC 2 cut(s) 193, 720
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 2 cut(s) 230, 284
AoxI GGCC 2 cut(s) 19, 483
ApaLI GTGCAC 1 cut(s) 541
AspLEI GCGC 2 cut(s) 517, 742
AspS9I GGNCC 1 cut(s) 857
AsuC2I CCSGG 1 cut(s) 731
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 1 cut(s) 857
BaeGI GKGCMC 1 cut(s) 545
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BanI GGYRCC 1 cut(s) 514
Bbv12I GWGCWC 2 cut(s) 545, 630
BccI CCATC 2 cut(s) 143, 429
BceAI ACGGC 6 cut(s) 34, 357, 470, 571, 646, 670
BcnI CCSGG 1 cut(s) 731
BcoDI GTCTC 2 cut(s) 193, 720
BfaI CTAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 518
BglII AGATCT 1 cut(s) 562
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 1 cut(s) 857
BmeRI GACNNNNNGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 857
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 731
BmsI GCATC 2 cut(s) 288, 457
Bpu10I CCTNAGC 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 731
BsaHI GRCGYC 2 cut(s) 330, 515
BsaI GGTCTC 1 cut(s) 720
BsaWI WCCGGW 1 cut(s) 827
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 736, 834
Bse1I ACTGG 2 cut(s) 22, 901
Bse3DI GCAATG 1 cut(s) 805
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 3 cut(s) 299, 736, 834
BseMI GCAATG 1 cut(s) 805
BseMII CTCAG 3 cut(s) 204, 452, 601
BseNI ACTGG 2 cut(s) 22, 901
BseRI GAGGAG 2 cut(s) 148, 416
BseSI GKGCMC 1 cut(s) 545
Bsh1236I CGCG 3 cut(s) 46, 342, 742
BshFI GGCC 2 cut(s) 21, 485
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 545, 630
BsiSI CCGG 2 cut(s) 731, 828
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 3 cut(s) 299, 736, 834
BsmAI GTCTC 2 cut(s) 193, 720
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 2 cut(s) 21, 485
Bso31I GGTCTC 1 cut(s) 720
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 545, 630
Bsp143I GATC 3 cut(s) 222, 289, 562
BspACI CCGC 2 cut(s) 46, 125
BspANI GGCC 2 cut(s) 21, 485
BspCNI CTCAG 3 cut(s) 203, 453, 600
BspFNI CGCG 3 cut(s) 46, 342, 742
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 2 cut(s) 230, 284
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 805
BsrI ACTGG 2 cut(s) 22, 901
BssMI GATC 3 cut(s) 222, 289, 562
BssNI GRCGYC 2 cut(s) 330, 515
Bst4CI ACNGT 4 cut(s) 209, 265, 353, 761
BstACI GRCGYC 2 cut(s) 330, 515
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 3 cut(s) 190, 461, 587
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 3 cut(s) 46, 342, 742
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 2 cut(s) 517, 742
BstKTI GATC 3 cut(s) 225, 292, 565
BstMAI GTCTC 2 cut(s) 193, 720
BstMBI GATC 3 cut(s) 222, 289, 562
BstMWI GCNNNNNNNGC 3 cut(s) 65, 122, 482
BstNSI RCATGY 3 cut(s) 182, 503, 701
BstSCI CCNGG 1 cut(s) 729
BstSLI GKGCMC 1 cut(s) 545
BstUI CGCG 3 cut(s) 46, 342, 742
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 2 cut(s) 21, 485
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 664
BtsIMutI CAGTG 2 cut(s) 626, 664
Cac8I GCNNGC 1 cut(s) 545
CfoI GCGC 2 cut(s) 517, 742
Cfr13I GGNCC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 80, 131, 364, 495, 765
CspCI CAANNNNNGTGG 2 cut(s) 419, 454
CviAII CATG 5 cut(s) 112, 179, 500, 575, 698
CviJI RGCY 7 cut(s) 21, 116, 372, 468, 485, 774, 910
CviKI_1 RGCY 7 cut(s) 21, 116, 372, 468, 485, 774, 910
CviQI GTAC 5 cut(s) 80, 131, 364, 495, 765
DdeI CTNAG 3 cut(s) 190, 461, 587
DinI GGCGCC 1 cut(s) 516
DpnI GATC 3 cut(s) 224, 291, 564
DpnII GATC 3 cut(s) 222, 289, 562
DraIII CACNNNGTG 1 cut(s) 823
DriI GACNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 2 cut(s) 19, 483
Eam1105I GACNNNNNGTC 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 720
Eco32I GATATC 1 cut(s) 683
Eco47I GGWCC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 905
EcoRV GATATC 1 cut(s) 683
EcoT22I ATGCAT 1 cut(s) 450
EgeI GGCGCC 1 cut(s) 516
EheI GGCGCC 1 cut(s) 516
FaeI CATG 5 cut(s) 115, 182, 503, 578, 701
FaqI GGGAC 1 cut(s) 315
FatI CATG 5 cut(s) 111, 178, 499, 574, 697
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 750
GlaI GCGC 2 cut(s) 516, 741
GluI GCNGC 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 2 cut(s) 21, 485
HapII CCGG 2 cut(s) 731, 828
HhaI GCGC 2 cut(s) 517, 742
Hin1I GRCGYC 2 cut(s) 330, 515
Hin1II CATG 5 cut(s) 115, 182, 503, 578, 701
Hin6I GCGC 2 cut(s) 515, 740
HinP1I GCGC 2 cut(s) 515, 740
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 3 cut(s) 201, 647, 838
HpaII CCGG 2 cut(s) 731, 828
Hpy166II GTNNAC 3 cut(s) 16, 364, 543
Hpy188I TCNGA 7 cut(s) 193, 222, 462, 652, 723, 771, 837
Hpy188III TCNNGA 4 cut(s) 319, 394, 864, 936
Hpy8I GTNNAC 3 cut(s) 16, 364, 543
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 1 cut(s) 870
HpyCH4III ACNGT 4 cut(s) 209, 265, 353, 761
HpyCH4IV ACGT 2 cut(s) 330, 675
HpyCH4V TGCA 8 cut(s) 74, 182, 246, 279, 414, 448, 543, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 122, 482
HpyF3I CTNAG 3 cut(s) 190, 461, 587
HpySE526I ACGT 2 cut(s) 330, 675
Hsp92I GRCGYC 2 cut(s) 330, 515
Hsp92II CATG 5 cut(s) 115, 182, 503, 578, 701
HspAI GCGC 2 cut(s) 515, 740
KasI GGCGCC 1 cut(s) 514
Kzo9I GATC 3 cut(s) 222, 289, 562
LmnI GCTCC 2 cut(s) 625, 771
LpnPI CCDG 9 cut(s) 35, 477, 574, 594, 744, 827, 841, 882, 896
LweI GCATC 2 cut(s) 288, 457
MaeI CTAG 1 cut(s) 750
MaeII ACGT 2 cut(s) 330, 675
MaeIII GTNAC 5 cut(s) 31, 48, 326, 487, 743
MalI GATC 3 cut(s) 224, 291, 564
MboI GATC 3 cut(s) 222, 289, 562
MboII GAAGA 3 cut(s) 16, 557, 898
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 2 cut(s) 545, 630
MluCI AATT 4 cut(s) 134, 183, 233, 523
Mly113I GGCGCC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 903
MnlI CCTC 5 cut(s) 93, 169, 394, 458, 794
Mph1103I ATGCAT 1 cut(s) 450
MseI TTAA 1 cut(s) 297
MslI CAYNNNNRTG 2 cut(s) 110, 354
MspI CCGG 2 cut(s) 731, 828
MspR9I CCNGG 1 cut(s) 731
MunI CAATTG 1 cut(s) 183
MvnI CGCG 3 cut(s) 46, 342, 742
MwoI GCNNNNNNNGC 3 cut(s) 65, 122, 482
NarI GGCGCC 1 cut(s) 515
NciI CCSGG 1 cut(s) 731
NdeII GATC 3 cut(s) 222, 289, 562
NlaIII CATG 5 cut(s) 115, 182, 503, 578, 701
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 2 cut(s) 326, 487
NsiI ATGCAT 1 cut(s) 450
NspI RCATGY 3 cut(s) 182, 503, 701
NspV TTCGAA 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 3 cut(s) 201, 647, 838
PkrI GCNGC 1 cut(s) 127
PluTI GGCGCC 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 516
PspPI GGNCC 1 cut(s) 857
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 5 cut(s) 81, 132, 365, 496, 766
RsaNI GTAC 5 cut(s) 80, 131, 364, 495, 765
RseI CAYNNNNRTG 2 cut(s) 110, 354
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 3 cut(s) 222, 289, 562
Sau96I GGNCC 1 cut(s) 857
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 2 cut(s) 545, 630
SfaNI GCATC 2 cut(s) 288, 457
SfoI GGCGCC 1 cut(s) 516
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 857
SmiMI CAYNNNNRTG 2 cut(s) 110, 354
Sse9I AATT 4 cut(s) 134, 183, 233, 523
SsiI CCGC 2 cut(s) 46, 125
SspDI GGCGCC 1 cut(s) 514
SspMI CTAG 1 cut(s) 750
StyD4I CCNGG 1 cut(s) 729
TaaI ACNGT 4 cut(s) 209, 265, 353, 761
TaiI ACGT 2 cut(s) 333, 678
TaqI TCGA 5 cut(s) 141, 204, 387, 672, 786
TasI AATT 4 cut(s) 134, 183, 233, 523
TatI WGTACW 2 cut(s) 130, 764
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 3 cut(s) 201, 647, 838
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 2 cut(s) 626, 664
TseFI GTSAC 2 cut(s) 326, 487
Tsp45I GTSAC 2 cut(s) 326, 487
TspDTI ATGAA 3 cut(s) 17, 543, 563
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 2 cut(s) 626, 664
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 857
XceI RCATGY 3 cut(s) 182, 503, 701
XspI CTAG 1 cut(s) 750
ZraI GACGTC 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.