DNA binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 875 |
| AccB1I | GGYRCC | 1 cut(s) | 875 |
| AccI | GTMKAC | 1 cut(s) | 360 |
| AciI | CCGC | 1 cut(s) | 364 |
| AcsI | RAATTY | 1 cut(s) | 861 |
| AcuI | CTGAAG | 1 cut(s) | 92 |
| AcyI | GRCGYC | 1 cut(s) | 504 |
| AfaI | GTAC | 4 cut(s) | 233, 650, 870, 877 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 695, 885 |
| AgsI | TTSAA | 6 cut(s) | 37, 58, 121, 148, 520, 620 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 843 |
| AleI | CACNNNNGTG | 1 cut(s) | 158 |
| AluBI | AGCT | 7 cut(s) | 7, 31, 106, 127, 136, 265, 312 |
| AluI | AGCT | 7 cut(s) | 7, 31, 106, 127, 136, 265, 312 |
| Alw21I | GWGCWC | 3 cut(s) | 33, 267, 1072 |
| Alw26I | GTCTC | 2 cut(s) | 340, 942 |
| Ama87I | CYCGRG | 2 cut(s) | 8, 1065 |
| AoxI | GGCC | 2 cut(s) | 88, 855 |
| ApaI | GGGCCC | 1 cut(s) | 859 |
| ApeKI | GCWGC | 3 cut(s) | 127, 237, 512 |
| ApoI | RAATTY | 1 cut(s) | 861 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 384, 416 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 705 |
| Asp718I | GGTACC | 1 cut(s) | 875 |
| AspS9I | GGNCC | 3 cut(s) | 88, 855, 856 |
| AsuHPI | GGTGA | 1 cut(s) | 217 |
| AvaI | CYCGRG | 2 cut(s) | 8, 1065 |
| BaeGI | GKGCMC | 1 cut(s) | 859 |
| BanI | GGYRCC | 1 cut(s) | 875 |
| BanII | GRGCYC | 3 cut(s) | 33, 267, 859 |
| BauI | CACGAG | 1 cut(s) | 883 |
| BbsI | GAAGAC | 1 cut(s) | 65 |
| Bbv12I | GWGCWC | 3 cut(s) | 33, 267, 1072 |
| BbvI | GCAGC | 3 cut(s) | 114, 224, 499 |
| BccI | CCATC | 4 cut(s) | 106, 253, 278, 494 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 518, 552 |
| BclI | TGATCA | 1 cut(s) | 976 |
| BcoDI | GTCTC | 2 cut(s) | 340, 942 |
| BfaI | CTAG | 1 cut(s) | 872 |
| BfmI | CTRYAG | 2 cut(s) | 128, 631 |
| BglII | AGATCT | 2 cut(s) | 51, 73 |
| BisI | GCNGC | 3 cut(s) | 128, 238, 513 |
| BlsI | GCNGC | 3 cut(s) | 129, 239, 514 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 843 |
| BmeT110I | CYCGRG | 2 cut(s) | 8, 1065 |
| BmgT120I | GGNCC | 3 cut(s) | 88, 855, 856 |
| BmiI | GGNNCC | 2 cut(s) | 857, 877 |
| BpiI | GAAGAC | 1 cut(s) | 65 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 884, 916, 1031, 1063 |
| BpmI | CTGGAG | 1 cut(s) | 843 |
| BpuEI | CTTGAG | 2 cut(s) | 122, 967 |
| BsaHI | GRCGYC | 1 cut(s) | 504 |
| BsaI | GGTCTC | 1 cut(s) | 942 |
| BsaJI | CCNNGG | 3 cut(s) | 24, 63, 156 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 695, 885 |
| Bse118I | RCCGGY | 1 cut(s) | 878 |
| Bse3DI | GCAATG | 1 cut(s) | 87 |
| BseDI | CCNNGG | 3 cut(s) | 24, 63, 156 |
| BseGI | GGATG | 1 cut(s) | 726 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 695, 885 |
| BseMI | GCAATG | 1 cut(s) | 87 |
| BseMII | CTCAG | 1 cut(s) | 915 |
| BseRI | GAGGAG | 4 cut(s) | 223, 235, 900, 1052 |
| BseSI | GKGCMC | 1 cut(s) | 859 |
| BseXI | GCAGC | 3 cut(s) | 114, 224, 499 |
| Bsh1285I | CGRYCG | 1 cut(s) | 364 |
| BshFI | GGCC | 2 cut(s) | 90, 857 |
| BshNI | GGYRCC | 1 cut(s) | 875 |
| BsiEI | CGRYCG | 1 cut(s) | 364 |
| BsiHKAI | GWGCWC | 3 cut(s) | 33, 267, 1072 |
| BsiHKCI | CYCGRG | 2 cut(s) | 8, 1065 |
| BsiSI | CCGG | 1 cut(s) | 879 |
| BslFI | GGGAC | 1 cut(s) | 890 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 695, 885 |
| BsmAI | GTCTC | 2 cut(s) | 340, 942 |
| BsmFI | GGGAC | 1 cut(s) | 890 |
| BsnI | GGCC | 2 cut(s) | 90, 857 |
| Bso31I | GGTCTC | 1 cut(s) | 942 |
| BsoBI | CYCGRG | 2 cut(s) | 8, 1065 |
| Bsp120I | GGGCCC | 1 cut(s) | 855 |
| Bsp1286I | GDGCHC | 4 cut(s) | 33, 267, 859, 1072 |
| Bsp143I | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| Bsp19I | CCATGG | 1 cut(s) | 156 |
| BspACI | CCGC | 1 cut(s) | 364 |
| BspANI | GGCC | 2 cut(s) | 90, 857 |
| BspCNI | CTCAG | 1 cut(s) | 914 |
| BspLI | GGNNCC | 2 cut(s) | 857, 877 |
| BspMAI | CTGCAG | 1 cut(s) | 132 |
| BspT107I | GGYRCC | 1 cut(s) | 875 |
| BspTNI | GGTCTC | 1 cut(s) | 942 |
| BsrDI | GCAATG | 1 cut(s) | 87 |
| BsrFI | RCCGGY | 1 cut(s) | 878 |
| BssAI | RCCGGY | 1 cut(s) | 878 |
| BssECI | CCNNGG | 3 cut(s) | 24, 63, 156 |
| BssMI | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| BssNI | GRCGYC | 1 cut(s) | 504 |
| BssSI | CACGAG | 1 cut(s) | 883 |
| BssT1I | CCWWGG | 3 cut(s) | 24, 63, 156 |
| Bst2BI | CACGAG | 1 cut(s) | 883 |
| Bst4CI | ACNGT | 1 cut(s) | 648 |
| Bst6I | CTCTTC | 2 cut(s) | 515, 1065 |
| BstACI | GRCGYC | 1 cut(s) | 504 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 961 |
| BstDEI | CTNAG | 4 cut(s) | 326, 679, 851, 901 |
| BstDSI | CCRYGG | 1 cut(s) | 156 |
| BstF5I | GGATG | 1 cut(s) | 726 |
| BstKTI | GATC | 6 cut(s) | 54, 76, 430, 570, 755, 979 |
| BstMAI | GTCTC | 2 cut(s) | 340, 942 |
| BstMBI | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| BstMCI | CGRYCG | 1 cut(s) | 364 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 103, 133, 262, 961 |
| BstNSI | RCATGY | 2 cut(s) | 391, 1076 |
| BstSFI | CTRYAG | 2 cut(s) | 128, 631 |
| BstSLI | GKGCMC | 1 cut(s) | 859 |
| BstV1I | GCAGC | 3 cut(s) | 114, 224, 499 |
| BstV2I | GAAGAC | 1 cut(s) | 65 |
| BstX2I | RGATCY | 2 cut(s) | 51, 73 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 160 |
| BstYI | RGATCY | 2 cut(s) | 51, 73 |
| BsuRI | GGCC | 2 cut(s) | 90, 857 |
| BtgI | CCRYGG | 1 cut(s) | 156 |
| BtsCI | GGATG | 1 cut(s) | 726 |
| BtsIMutI | CAGTG | 1 cut(s) | 720 |
| Cfr10I | RCCGGY | 1 cut(s) | 878 |
| Cfr13I | GGNCC | 3 cut(s) | 88, 855, 856 |
| CseI | GACGC | 1 cut(s) | 493 |
| Csp6I | GTAC | 4 cut(s) | 232, 649, 869, 876 |
| CviAII | CATG | 7 cut(s) | 157, 241, 388, 672, 932, 944, 1073 |
| CviQI | GTAC | 4 cut(s) | 232, 649, 869, 876 |
| DdeI | CTNAG | 4 cut(s) | 326, 679, 851, 901 |
| DpnI | GATC | 6 cut(s) | 53, 75, 429, 569, 754, 978 |
| DpnII | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| DraI | TTTAAA | 1 cut(s) | 693 |
| DriI | GACNNNNNGTC | 1 cut(s) | 843 |
| Eam1104I | CTCTTC | 2 cut(s) | 515, 1065 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 843 |
| EarI | CTCTTC | 2 cut(s) | 515, 1065 |
| Ecl136II | GAGCTC | 2 cut(s) | 31, 265 |
| Eco130I | CCWWGG | 3 cut(s) | 24, 63, 156 |
| Eco24I | GRGCYC | 3 cut(s) | 33, 267, 859 |
| Eco31I | GGTCTC | 1 cut(s) | 942 |
| Eco53kI | GAGCTC | 2 cut(s) | 31, 265 |
| Eco57I | CTGAAG | 1 cut(s) | 92 |
| Eco88I | CYCGRG | 2 cut(s) | 8, 1065 |
| EcoICRI | GAGCTC | 2 cut(s) | 31, 265 |
| EcoO109I | RGGNCCY | 1 cut(s) | 855 |
| EcoT14I | CCWWGG | 3 cut(s) | 24, 63, 156 |
| EcoT22I | ATGCAT | 1 cut(s) | 937 |
| EcoT38I | GRGCYC | 3 cut(s) | 33, 267, 859 |
| ErhI | CCWWGG | 3 cut(s) | 24, 63, 156 |
| FaeI | CATG | 7 cut(s) | 160, 244, 391, 675, 935, 947, 1076 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 90, 122 |
| FaqI | GGGAC | 1 cut(s) | 890 |
| FatI | CATG | 7 cut(s) | 156, 240, 387, 671, 931, 943, 1072 |
| FbaI | TGATCA | 1 cut(s) | 976 |
| FblI | GTMKAC | 1 cut(s) | 360 |
| Fnu4HI | GCNGC | 3 cut(s) | 128, 238, 513 |
| FokI | GGATG | 1 cut(s) | 713 |
| FriOI | GRGCYC | 3 cut(s) | 33, 267, 859 |
| Fsp4HI | GCNGC | 3 cut(s) | 128, 238, 513 |
| FspBI | CTAG | 1 cut(s) | 872 |
| GluI | GCNGC | 3 cut(s) | 128, 238, 513 |
| GsuI | CTGGAG | 1 cut(s) | 843 |
| HaeIII | GGCC | 2 cut(s) | 90, 857 |
| HapII | CCGG | 1 cut(s) | 879 |
| HgaI | GACGC | 1 cut(s) | 493 |
| Hin1I | GRCGYC | 1 cut(s) | 504 |
| Hin1II | CATG | 7 cut(s) | 160, 244, 391, 675, 935, 947, 1076 |
| HincII | GTYRAC | 4 cut(s) | 361, 508, 644, 848 |
| HindII | GTYRAC | 4 cut(s) | 361, 508, 644, 848 |
| HindIII | AAGCTT | 1 cut(s) | 104 |
| HinfI | GANTC | 2 cut(s) | 808, 833 |
| HpaII | CCGG | 1 cut(s) | 879 |
| HphI | GGTGA | 1 cut(s) | 217 |
| Hpy166II | GTNNAC | 5 cut(s) | 361, 508, 561, 644, 848 |
| Hpy188I | TCNGA | 4 cut(s) | 111, 219, 427, 550 |
| Hpy188III | TCNNGA | 6 cut(s) | 10, 34, 55, 443, 620, 822 |
| Hpy8I | GTNNAC | 5 cut(s) | 361, 508, 561, 644, 848 |
| Hpy99I | CGWCG | 2 cut(s) | 642, 841 |
| HpyAV | CCTTC | 3 cut(s) | 7, 31, 79 |
| HpyCH4III | ACNGT | 1 cut(s) | 648 |
| HpyCH4V | TGCA | 8 cut(s) | 130, 240, 251, 539, 759, 935, 941, 1076 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 103, 133, 262, 961 |
| HpyF3I | CTNAG | 4 cut(s) | 326, 679, 851, 901 |
| Hsp92I | GRCGYC | 1 cut(s) | 504 |
| Hsp92II | CATG | 7 cut(s) | 160, 244, 391, 675, 935, 947, 1076 |
| KpnI | GGTACC | 1 cut(s) | 879 |
| Ksp22I | TGATCA | 1 cut(s) | 976 |
| Kzo9I | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| LmnI | GCTCC | 4 cut(s) | 28, 210, 262, 270 |
| LpnPI | CCDG | 5 cut(s) | 807, 808, 892, 965, 987 |
| Lsp1109I | GCAGC | 3 cut(s) | 114, 224, 499 |
| MaeI | CTAG | 1 cut(s) | 872 |
| MaeIII | GTNAC | 1 cut(s) | 376 |
| MalI | GATC | 6 cut(s) | 53, 75, 429, 569, 754, 978 |
| MboI | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| MflI | RGATCY | 2 cut(s) | 51, 73 |
| MhlI | GDGCHC | 4 cut(s) | 33, 267, 859, 1072 |
| MluCI | AATT | 3 cut(s) | 731, 861, 916 |
| MnlI | CCTC | 8 cut(s) | 201, 213, 291, 910, 918, 921, 1030, 1068 |
| Mph1103I | ATGCAT | 1 cut(s) | 937 |
| MroXI | GAANNNNTTC | 1 cut(s) | 705 |
| MseI | TTAA | 2 cut(s) | 692, 920 |
| MslI | CAYNNNNRTG | 1 cut(s) | 158 |
| MspI | CCGG | 1 cut(s) | 879 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 103, 133, 262, 961 |
| NcoI | CCATGG | 1 cut(s) | 156 |
| NdeII | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| NlaIII | CATG | 7 cut(s) | 160, 244, 391, 675, 935, 947, 1076 |
| NlaIV | GGNNCC | 2 cut(s) | 857, 877 |
| NmeAIII | GCCGAG | 1 cut(s) | 116 |
| NsiI | ATGCAT | 1 cut(s) | 937 |
| NspI | RCATGY | 2 cut(s) | 391, 1076 |
| OliI | CACNNNNGTG | 1 cut(s) | 158 |
| PaeR7I | CTCGAG | 1 cut(s) | 8 |
| PdmI | GAANNNNTTC | 1 cut(s) | 705 |
| PfeI | GAWTC | 2 cut(s) | 808, 833 |
| PkrI | GCNGC | 3 cut(s) | 129, 239, 514 |
| Psp124BI | GAGCTC | 2 cut(s) | 33, 267 |
| PspN4I | GGNNCC | 2 cut(s) | 857, 877 |
| PspOMI | GGGCCC | 1 cut(s) | 855 |
| PspPI | GGNCC | 3 cut(s) | 88, 855, 856 |
| PstI | CTGCAG | 1 cut(s) | 132 |
| PsuI | RGATCY | 2 cut(s) | 51, 73 |
| RsaI | GTAC | 4 cut(s) | 233, 650, 870, 877 |
| RsaNI | GTAC | 4 cut(s) | 232, 649, 869, 876 |
| RseI | CAYNNNNRTG | 1 cut(s) | 158 |
| SacI | GAGCTC | 2 cut(s) | 33, 267 |
| SalI | GTCGAC | 1 cut(s) | 359 |
| SaqAI | TTAA | 2 cut(s) | 692, 920 |
| SatI | GCNGC | 3 cut(s) | 128, 238, 513 |
| Sau3AI | GATC | 6 cut(s) | 51, 73, 427, 567, 752, 976 |
| Sau96I | GGNCC | 3 cut(s) | 88, 855, 856 |
| SduI | GDGCHC | 4 cut(s) | 33, 267, 859, 1072 |
| SfcI | CTRYAG | 2 cut(s) | 128, 631 |
| Sfr274I | CTCGAG | 1 cut(s) | 8 |
| SlaI | CTCGAG | 1 cut(s) | 8 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 158 |
| SmlI | CTYRAG | 3 cut(s) | 8, 137, 982 |
| SmoI | CTYRAG | 3 cut(s) | 8, 137, 982 |
| Sse9I | AATT | 3 cut(s) | 731, 861, 916 |
| SsiI | CCGC | 1 cut(s) | 364 |
| SspMI | CTAG | 1 cut(s) | 872 |
| SstI | GAGCTC | 2 cut(s) | 33, 267 |
| StyI | CCWWGG | 3 cut(s) | 24, 63, 156 |
| TaaI | ACNGT | 1 cut(s) | 648 |
| TaqI | TCGA | 5 cut(s) | 9, 273, 360, 836, 1049 |
| TasI | AATT | 3 cut(s) | 731, 861, 916 |
| TatI | WGTACW | 1 cut(s) | 868 |
| TfiI | GAWTC | 2 cut(s) | 808, 833 |
| Tru1I | TTAA | 2 cut(s) | 692, 920 |
| Tru9I | TTAA | 2 cut(s) | 692, 920 |
| TscAI | CASTG | 1 cut(s) | 727 |
| TseI | GCWGC | 3 cut(s) | 127, 237, 512 |
| TspDTI | ATGAA | 5 cut(s) | 17, 98, 257, 567, 843 |
| TspGWI | ACGGA | 1 cut(s) | 803 |
| TspRI | CASTG | 1 cut(s) | 727 |
| XapI | RAATTY | 1 cut(s) | 861 |
| XceI | RCATGY | 2 cut(s) | 391, 1076 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 163 |
| XhoI | CTCGAG | 1 cut(s) | 8 |
| XmiI | GTMKAC | 1 cut(s) | 360 |
| XmnI | GAANNNNTTC | 1 cut(s) | 705 |
| XspI | CTAG | 1 cut(s) | 872 |
| Zsp2I | ATGCAT | 1 cut(s) | 937 |