FvH4_2g17621

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000159
Physical Location & Seq
Reverse (-)
15149604 .. 15151939
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17621.t1

Sequence Viewer

Length: 1923 bp
ATGCCACTTTTTTCGCGTTGGAATCCAGTAGTGACTAACTTCAATCTGGGACATAAAGTGAAAGAAATTAGAGAGCGACTAACTGAAATTGAGAAAGATAAGATTGAGTTTGATCTCGTTGAGATGACCAAGATAGCTGATGATCATAGTGTGCCCCAAGGGATGCAATACAATCAAAGAGAAACTGATTCCTTGGTAGAGGCTTCAGGAGTTATTGGGAGAGATGATGATAAAGAGCTAATTTTTAGTCATCTCTTACATAACACTGATATCTCTAATGAGGATAATGTTTATGTTATTTCAATCCTTGGGATGGGGGGATTGGGTAAGACAACTCTGGCTAAATTGGTGTACAATGATAGCGTGGTAGAGGAGAATTTTGAGGTGAAAATGTGGGTATGTGTCTCAGAAAACTTTGAAGTTAAATCATTAATTCTCAAGAATATCAATGCTATAACTGATCAAAAATGTGAGGATGAAAGTTTGGACCGTATGAAAAGAAGTTTGCAAGATACTTTGAGAGGTAGAAAGTTTTTGTTAGTGTTAGATGATGTTTGGGATACGGAGTCTATTGGAGTAACACCAGAAAAATGGTTGGGTTTAAAAAGTCTGTTAAGTGTGGGAGCTAATGGAAGCAGAATCCTCGTAACAACACGAAATAGATCAATTGCTTTACTCGTGAATCTTTCATACATGCATTCTTTGAAAGGTCTTTCCCACAAAGACTACATATCCTTGTTCATCAAAAGGGCATTCAAGAAAGGAGAGGAGCAATGCTATCCACGCTTGATAGAAATTGGAGAGGATATTGTGAACAAGTGTGGAGGAGTTCCTTTAGCTGTAGCAACTCTAGGGAGCATGCTCTATTTGAACAGAGAGCCACACCGATGGTTGAGTGTGAGAGATGATGACATGTGGAGTATAGGAAATGATCATATTCTACCTACACTCAAATTGAGCTATGATGCATTGCCACAACACTTGAAACCATGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTTTGAATTCCATGGTGACTTTCTGGTTTACCTGTGGGTAGCACAAGGCTACCTCAAAACATGTAAAAAAAATGAAGATCTTGATAAAATGGGCATGGACTATATAATACATTTCTGCTCTAGATCATTGTTTCAAGTTGCAAACGATTTAAGAACCAGAATGATATTTAAAATGCATGATCTAGTTCATGATATAGCAATTTCAGTGGCACAAGCAGAATACTCCACAGTCAATTTTCGTCCCTCTAGTGCTTTTGAAATGGTTCGACATGTGTCAATATCCGAAAAGGACTTGCTTGGAGAAGAAGCACAAGTGCCTGATTTCTTATTCAAGTCAACCAAATTACGAACCTTTCTAATTCCATATGGAGATGGCAAGATGATGAATCAACATTTTGTGAAGACATGCATCTCGAGATTCAAATATATGCGCGTGCTAGATGTCAGTGGGTCGCCCCTTGAGAAGCTACCAAGTTCCATTGGTAGTTTGTTTCATCTGAGATTTCTCGACTTGTCTTGTAATAATTTGGAGGCTTTGCCTTCCTGGCTTGAAGATTATGCAATAGTTTTGCAGAGCATAGCAGTCAAACAGTGTGATAATTTGACTGAACTTCCGGAATTGCAAATATTTACATTCCTCGAGCAACTGTTGATTAAAAGTTGTTCCAATTTGTTAGCTTTACCGCAAGGGTTACATTGCCTCACTGAGTTGAGAGAATTGAAAATTAATGGTTGTCCCAAACTGAGCAAGAGCTGCAAAAGGCAATTAAAAGGTGAGAATTGGTCAAAGTTCGCACGTGAGGCAAAGATTGAACTCGACTCTGATGTGGATACAAATGAAGAGGAAGACAAAGATGAAGGTGTTTTCGACGATCACAATGCTATCTCAAGTGATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

641

Amino Acids

73.15

Weight (kDa)

5.55

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 92 - 254 8.8e-32 NB-ARC domain
WHD_DRP PF23559 337 - 407 1.7e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 476 - 586 2.2e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 16, 1455
AccIII TCCGGA 1 cut(s) 1636
AciI CCGC 1 cut(s) 1706
AcsI RAATTY 2 cut(s) 376, 1028
AcuI CTGAAG 1 cut(s) 189
AcvI CACGTG 1 cut(s) 1820
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 3 cut(s) 912, 1082, 1291
AjnI CCWGG 1 cut(s) 1565
AluBI AGCT 8 cut(s) 137, 238, 626, 839, 960, 1489, 1700, 1776
AluI AGCT 8 cut(s) 137, 238, 626, 839, 960, 1489, 1700, 1776
Alw26I GTCTC 1 cut(s) 409
Ama87I CYCGRG 2 cut(s) 1435, 1661
Aor13HI TCCGGA 1 cut(s) 1636
ApeKI GCWGC 1 cut(s) 1776
ApoI RAATTY 2 cut(s) 376, 1028
AseI ATTAAT 2 cut(s) 431, 1749
Asp700I GAANNNNTTC 1 cut(s) 1284
AspLEI GCGC 1 cut(s) 1455
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 3 cut(s) 397, 1049, 1808
AvaI CYCGRG 2 cut(s) 1435, 1661
AvaII GGWCC 1 cut(s) 487
BaeGI GKGCMC 1 cut(s) 156
BauI CACGAG 1 cut(s) 677
BbrPI CACGTG 1 cut(s) 1820
BbsI GAAGAC 2 cut(s) 1430, 1875
BbvI GCAGC 1 cut(s) 1763
BccI CCATC 3 cut(s) 307, 882, 1388
BcgI CGANNNNNNTGC 4 cut(s) 625, 659, 1883, 1917
BciT130I CCWGG 1 cut(s) 1567
BciVI GTATCC 2 cut(s) 553, 1846
BclI TGATCA 3 cut(s) 142, 460, 931
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 5 cut(s) 851, 1143, 1205, 1269, 1460
BfmI CTRYAG 1 cut(s) 840
BfuI GTATCC 2 cut(s) 553, 1846
BglI GCCNNNNNGGC 1 cut(s) 1567
BglII AGATCT 1 cut(s) 1099
BisI GCNGC 1 cut(s) 1777
BlsI GCNGC 1 cut(s) 1778
Bme1390I CCNGG 1 cut(s) 1567
Bme18I GGWCC 1 cut(s) 487
BmeT110I CYCGRG 2 cut(s) 1435, 1661
BmgT120I GGNCC 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 1567
BmsI GCATC 3 cut(s) 153, 955, 1440
BoxI GACNNNNGTC 1 cut(s) 1294
BpiI GAAGAC 2 cut(s) 1430, 1875
BpuEI CTTGAG 3 cut(s) 422, 1502, 1894
BsaAI YACGTR 1 cut(s) 1820
BsaJI CCNNGG 4 cut(s) 157, 192, 307, 1033
BsaWI WCCGGW 1 cut(s) 1636
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 3 cut(s) 779, 968, 1717
BseAI TCCGGA 1 cut(s) 1636
BseBI CCWGG 1 cut(s) 1567
BseDI CCNNGG 4 cut(s) 157, 192, 307, 1033
BseGI GGATG 3 cut(s) 168, 318, 481
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 3 cut(s) 779, 968, 1717
BseMII CTCAG 4 cut(s) 420, 1511, 1719, 1757
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 3 cut(s) 386, 782, 840
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 1 cut(s) 1763
Bsh1236I CGCG 2 cut(s) 16, 1455
BsiHKCI CYCGRG 2 cut(s) 1435, 1661
BsiSI CCGG 1 cut(s) 1637
BslFI GGGAC 3 cut(s) 63, 1248, 1743
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 409
BsmFI GGGAC 3 cut(s) 63, 1248, 1743
BsmI GAATGC 2 cut(s) 697, 752
BsoBI CYCGRG 2 cut(s) 1435, 1661
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 1636
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 1 cut(s) 1706
BspCNI CTCAG 4 cut(s) 419, 1512, 1720, 1758
BspEI TCCGGA 1 cut(s) 1636
BspFNI CGCG 2 cut(s) 16, 1455
BspHI TCATGA 1 cut(s) 1210
BsrDI GCAATG 3 cut(s) 779, 968, 1717
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 4 cut(s) 157, 192, 307, 1033
BssMI GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
BssSI CACGAG 1 cut(s) 677
BssT1I CCWWGG 4 cut(s) 157, 192, 307, 1033
Bst2BI CACGAG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 1567
Bst4CI ACNGT 4 cut(s) 491, 1252, 1614, 1671
Bst6I CTCTTC 1 cut(s) 1857
BstAPI GCANNNNNTGC 1 cut(s) 1776
BstAUI TGTACA 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 1820
BstC8I GCNNGC 2 cut(s) 860, 1457
BstDEI CTNAG 4 cut(s) 406, 1520, 1728, 1766
BstDSI CCRYGG 1 cut(s) 1033
BstF5I GGATG 3 cut(s) 168, 318, 481
BstFNI CGCG 2 cut(s) 16, 1455
BstHHI GCGC 1 cut(s) 1455
BstKTI GATC 9 cut(s) 115, 145, 463, 665, 934, 1102, 1148, 1204, 1897
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
BstMWI GCNNNNNNNGC 4 cut(s) 783, 1567, 1776, 1823
BstNI CCWGG 1 cut(s) 1567
BstNSI RCATGY 6 cut(s) 697, 862, 916, 1086, 1295, 1431
BstPAI GACNNNNGTC 1 cut(s) 1294
BstSCI CCNGG 1 cut(s) 1565
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 2 cut(s) 16, 1455
BstV1I GCAGC 1 cut(s) 1763
BstV2I GAAGAC 2 cut(s) 1430, 1875
BstX2I RGATCY 1 cut(s) 1099
BstXI CCANNNNNNTGG 2 cut(s) 591, 888
BstYI RGATCY 1 cut(s) 1099
BsuI GTATCC 2 cut(s) 553, 1846
BtgI CCRYGG 1 cut(s) 1033
BtsCI GGATG 3 cut(s) 168, 318, 481
BtsIMutI CAGTG 5 cut(s) 264, 1233, 1474, 1619, 1725
Cac8I GCNNGC 2 cut(s) 860, 1457
CciI TCATGA 1 cut(s) 1210
CfoI GCGC 1 cut(s) 1455
Cfr13I GGNCC 1 cut(s) 487
Csp6I GTAC 1 cut(s) 352
CspCI CAANNNNNGTGG 4 cut(s) 963, 998, 1209, 1244
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 4 cut(s) 406, 1520, 1728, 1766
DpnI GATC 9 cut(s) 114, 144, 462, 664, 933, 1101, 1147, 1203, 1896
DpnII GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
DraI TTTAAA 2 cut(s) 603, 1192
Eam1104I CTCTTC 1 cut(s) 1857
EarI CTCTTC 1 cut(s) 1857
Eco130I CCWWGG 4 cut(s) 157, 192, 307, 1033
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 189
Eco72I CACGTG 1 cut(s) 1820
Eco88I CYCGRG 2 cut(s) 1435, 1661
EcoRI GAATTC 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 1565
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 4 cut(s) 157, 192, 307, 1033
EcoT22I ATGCAT 4 cut(s) 699, 970, 1200, 1433
ErhI CCWWGG 4 cut(s) 157, 192, 307, 1033
FaqI GGGAC 3 cut(s) 63, 1248, 1743
FauNDI CATATG 1 cut(s) 1387
FbaI TGATCA 3 cut(s) 142, 460, 931
Fnu4HI GCNGC 1 cut(s) 1777
FokI GGATG 3 cut(s) 175, 325, 488
Fsp4HI GCNGC 1 cut(s) 1777
FspBI CTAG 5 cut(s) 851, 1143, 1205, 1269, 1460
GlaI GCGC 1 cut(s) 1454
GluI GCNGC 1 cut(s) 1777
HapII CCGG 1 cut(s) 1637
HhaI GCGC 1 cut(s) 1455
Hin6I GCGC 1 cut(s) 1453
HinP1I GCGC 1 cut(s) 1453
HincII GTYRAC 1 cut(s) 1359
HindII GTYRAC 1 cut(s) 1359
HinfI GANTC 8 cut(s) 22, 188, 566, 639, 682, 1408, 1440, 1841
HpaII CCGG 1 cut(s) 1637
HphI GGTGA 3 cut(s) 397, 1049, 1808
Hpy166II GTNNAC 5 cut(s) 352, 814, 1007, 1051, 1359
Hpy188I TCNGA 4 cut(s) 409, 1306, 1521, 1846
Hpy8I GTNNAC 5 cut(s) 352, 814, 1007, 1051, 1359
Hpy99I CGWCG 1 cut(s) 1895
HpyAV CCTTC 2 cut(s) 1572, 1874
HpyCH4III ACNGT 4 cut(s) 491, 1252, 1614, 1671
HpyCH4IV ACGT 1 cut(s) 1819
HpyF10VI GCNNNNNNNGC 4 cut(s) 783, 1567, 1776, 1823
HpyF3I CTNAG 4 cut(s) 406, 1520, 1728, 1766
HpySE526I ACGT 1 cut(s) 1819
HspAI GCGC 1 cut(s) 1453
Kpn2I TCCGGA 1 cut(s) 1636
Ksp22I TGATCA 3 cut(s) 142, 460, 931
Kzo9I GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
LmnI GCTCC 3 cut(s) 623, 769, 855
Lsp1109I GCAGC 1 cut(s) 1763
LweI GCATC 3 cut(s) 153, 955, 1440
MaeI CTAG 5 cut(s) 851, 1143, 1205, 1269, 1460
MaeII ACGT 1 cut(s) 1819
MaeIII GTNAC 5 cut(s) 31, 577, 646, 1037, 1713
MalI GATC 9 cut(s) 114, 144, 462, 664, 933, 1101, 1147, 1203, 1896
MboI GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
MboII GAAGA 6 cut(s) 1109, 1337, 1435, 1586, 1874, 1880
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 1099
MhlI GDGCHC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 575, 1835
Mph1103I ATGCAT 4 cut(s) 699, 970, 1200, 1433
MroI TCCGGA 1 cut(s) 1636
MroXI GAANNNNTTC 1 cut(s) 1284
MseI TTAA 9 cut(s) 423, 431, 602, 614, 1172, 1191, 1677, 1749, 1790
MslI CAYNNNNRTG 1 cut(s) 886
MspI CCGG 1 cut(s) 1637
MspR9I CCNGG 1 cut(s) 1567
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 697, 752
MvaI CCWGG 1 cut(s) 1567
MvnI CGCG 2 cut(s) 16, 1455
MwoI GCNNNNNNNGC 4 cut(s) 783, 1567, 1776, 1823
NcoI CCATGG 1 cut(s) 1033
NdeI CATATG 1 cut(s) 1387
NdeII GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
NmuCI GTSAC 2 cut(s) 31, 1037
NsiI ATGCAT 4 cut(s) 699, 970, 1200, 1433
NspI RCATGY 6 cut(s) 697, 862, 916, 1086, 1295, 1431
PaeI GCATGC 1 cut(s) 862
PaeR7I CTCGAG 2 cut(s) 1435, 1661
PagI TCATGA 1 cut(s) 1210
PciI ACATGT 3 cut(s) 912, 1082, 1291
PctI GAATGC 2 cut(s) 697, 752
PdmI GAANNNNTTC 1 cut(s) 1284
PfeI GAWTC 6 cut(s) 22, 188, 639, 682, 1408, 1440
PkrI GCNGC 1 cut(s) 1778
PleI GAGTC 2 cut(s) 574, 1835
PmaCI CACGTG 1 cut(s) 1820
PmlI CACGTG 1 cut(s) 1820
PpsI GAGTC 2 cut(s) 574, 1835
Ppu21I YACGTR 1 cut(s) 1820
PscI ACATGT 3 cut(s) 912, 1082, 1291
PshAI GACNNNNGTC 1 cut(s) 1294
PshBI ATTAAT 2 cut(s) 431, 1749
Psp6I CCWGG 1 cut(s) 1565
PspCI CACGTG 1 cut(s) 1820
PspGI CCWGG 1 cut(s) 1565
PspPI GGNCC 1 cut(s) 487
PspXI VCTCGAGB 1 cut(s) 1661
PsuI RGATCY 1 cut(s) 1099
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 886
SaqAI TTAA 9 cut(s) 423, 431, 602, 614, 1172, 1191, 1677, 1749, 1790
SatI GCNGC 1 cut(s) 1777
Sau3AI GATC 9 cut(s) 112, 142, 460, 662, 931, 1099, 1145, 1201, 1894
Sau96I GGNCC 1 cut(s) 487
SchI GAGTC 2 cut(s) 575, 1835
ScrFI CCNGG 1 cut(s) 1567
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 3 cut(s) 153, 955, 1440
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 2 cut(s) 1435, 1661
SinI GGWCC 1 cut(s) 487
SlaI CTCGAG 2 cut(s) 1435, 1661
SmiMI CAYNNNNRTG 1 cut(s) 886
SmlI CTYRAG 5 cut(s) 437, 1435, 1481, 1661, 1909
SmoI CTYRAG 5 cut(s) 437, 1435, 1481, 1661, 1909
SphI GCATGC 1 cut(s) 862
SsiI CCGC 1 cut(s) 1706
SspI AATATT 1 cut(s) 1650
SspMI CTAG 5 cut(s) 851, 1143, 1205, 1269, 1460
StyD4I CCNGG 1 cut(s) 1565
StyI CCWWGG 4 cut(s) 157, 192, 307, 1033
TaaI ACNGT 4 cut(s) 491, 1252, 1614, 1671
TaiI ACGT 1 cut(s) 1822
TaqI TCGA 6 cut(s) 1288, 1436, 1530, 1662, 1839, 1890
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 6 cut(s) 22, 188, 639, 682, 1408, 1440
Tru1I TTAA 9 cut(s) 423, 431, 602, 614, 1172, 1191, 1677, 1749, 1790
Tru9I TTAA 9 cut(s) 423, 431, 602, 614, 1172, 1191, 1677, 1749, 1790
TscAI CASTG 5 cut(s) 271, 1233, 1474, 1619, 1732
TseFI GTSAC 2 cut(s) 31, 1037
TseI GCWGC 1 cut(s) 1776
Tsp45I GTSAC 2 cut(s) 31, 1037
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 5 cut(s) 271, 1233, 1474, 1619, 1732
VpaK11BI GGWCC 1 cut(s) 487
VspI ATTAAT 2 cut(s) 431, 1749
XapI RAATTY 2 cut(s) 376, 1028
XbaI TCTAGA 1 cut(s) 1142
XceI RCATGY 6 cut(s) 697, 862, 916, 1086, 1295, 1431
XhoI CTCGAG 2 cut(s) 1435, 1661
XmnI GAANNNNTTC 1 cut(s) 1284
XspI CTAG 5 cut(s) 851, 1143, 1205, 1269, 1460
Zsp2I ATGCAT 4 cut(s) 699, 970, 1200, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.