Rroxscaffold_3G00273210

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000159
Physical Location & Seq
Reverse (-)
65077990 .. 65081332
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273210.1

Sequence Viewer

Length: 2604 bp
ATGGAGTTTGCCACCGGCATTGCTGAGAACGTCTTGGGTAGGCTAGCCTCCTATGCTGCCCAAGAAGTTTGCTTGGCATGGGGAGCCCAACTTGAGTTGCAGAAGCTGCAGGAGACCTTGTTAGACATCAAAGACGTCCTCCAGGATGCGGAGGAAAAGCAAGTGCAGAATCCTTTAATCAGCCGTTGGTTAGGAGACCTCAGACATGTTTGCAACGATGCTGAGGATGTATTGGACGAATTTGAGTTCCAAAAGCTCCGACTCGAAGTGCTCATCAACAACCACCGAAGAGTCAAAGGAGAGGTACACCAATTCTTCTCCCGTTGGAATTCCGTTGTGTTTAACTTCAAATTGGGTCATAAATTAAGACGCATTAGGGAACGTCTAGTTCAAATTGATAATGATGGAAAGACCAAGTTTTCTCTCGTTATTAAAGTTGTTCAACAAACTGAAGTTTGTCCGGCACCCTTGACGTCGAGTGTTAATAGAAGGGAGACAGACTCTTTATTGGAATATCATGTTGTTGGAAGAGATGGTGATAAGGAAAACATTATCGGTCTCTTTGATGACACCGGTTCTTCCTGTGTCGATAATGTTTCAGTTATTTCCATCTTTGGGTTAGGAGGGTTGGGAAAAACTACGCTTGCTAAATCAGTGTATAATGATACTATGGTAGAGAACAAATTTGATATGAGAATGTGGGTATGCGTCTCGGAGAGCTTTGATATCCATACATTAGTCCGTGGCATTATTGCTGCAACTAAACAAAATTGTGCTGCTGAAAGTTTAGATCTTATGAAAAGGGGGTTGCAAGATATTTTGAGAGATAAAAAGTTTTTGTTAGTGTTGGACGATGTCTGGGATACAGAGTCAATAGGACTTACAATTCAAAAATGGAGTGAGTTAAAAACTTTGTTAGATGTTGGAGCCAAAGAGAGTAAAATCATTGTAACAACACGCAATGAATCAGTAGCTTCACTTGTGCAACCTATACACATGCATCATTTAAAAGGTCTTCCTCATGAGGATTCCATGACTTTGTTTAAGAAAAATGCATTTAATGAAGGAGAGGAGTCACACTATCAACACTTGATAAAAATTGGAGAGGACATTGTGAAGAAGTGTGGAGGAGTTCCCTTAGCACTAGCTACTCTAGGGAGTCTGCTTCGCTCGAAAAGGGAGCGACATCACTGGTTACAAGTAAGAGATGATGAAATATGGAGAATGTCAAGAGATGACAACATTATGGCTGCACTAAAATTGAGTTACAATGCACTGCCATCTCACTTGAAGCATTGTTTTGCCTTTTGTTCACTTTACCCCAAGGATTATGAATTTGAAAGTGAAGAAATAGTCCCACTATGGATGGCACAAGGCTTTCTCAAGTCCTTGAGAGAAAATGAAGATTTTAGCGAGATAGGCCTAGACTATATAAGACAATTTTGTTCCAAGTCTTTGTTTCAAATTGAGTTTGATACGAAGACTAATATGGGGTTCAAAATCCATGATTTATTGCACGATTTAGCAATATATGTGGCACGGGCAGAGTGCTCCACAATAAATTTTCGTCACTCTAGTGCCTTTGAAAAAGTCCGGCACGTGTCAATATCAAAAAAAGACTTGTCTAGAGAAGAGGCAGGAATCCCGGCATTCATTCTCCAGTCGAAGAAACTACGGACCATTCTAAATCTAGACAGTGAAGTTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCACTAGATGTGCGTGGTTCTACTCTTGAGGAGCTACCAAGTTCTATTGGTAACTTGATTCATTTAAGATATCTCAACCTGAAGGGGAATACAAGGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGCTCTTAAACCTTGACGGTTGTGTGGCACTTGAGGAGTTACCTAAAGGCATAGGAAACCTGATCAACCTTAGATTTTTGAATATAACTACACAGCAGATGTATTTTCCGAAAGGACAATTTAGACGCCTCACCTTACTTCAAGTTTTAGCCGTTGAGGGCAAGAATCTAAAATCTCTGGGCGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTGGATTTTTCACTGTGGAAGTATGGAGTCCTTGCCACCTAACTTGAAGAAATTGACTGCATTACATACTTTATTGATCGGTCATTGTAAGACAATACAGTTACTGAGGTGGGGAGAAGGCATCAAAGGTCTTCGATCAATTGTTATCCATGGTTGTTCAGATTTGGAGGCTTTGCCAAATTGGCTTCAAGAGTCTGCAGACACTTTACAGAGCATGGTAATCGCGCAGTGTGATAATCTCACAGCACTTCCAGAGTGGTTGCAAAATCTCGAGCAACTAGCGATTGCAGACTGCCCCAAATTGTCTGCTTTGCAGCAAGGGATGCATTGCCTCACTGCGTTGAGAGAATTGCATATTCACAGGTGTCCTGAATTGAGAAAAAGATGCAAAAGTCAAACGGGAGAGGATTGGCCCAAGATCAAACATGTCCCAAAGATTATACTCGACGACTCTGATGATGAATTTGCCAATGATATCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

867

Amino Acids

98.91

Weight (kDa)

6.51

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 93 1.9e-21 Rx N-terminal domain
NB-ARC PF00931 197 - 355 2.6e-34 NB-ARC domain
WHD_DRP PF23559 439 - 509 8.6e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 580 - 780 1.3e-27 Leucine-rich repeat region
LRR_13 PF23286 594 - 738 1.3e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 138, 476
AccB1I GGYRCC 1 cut(s) 463
AccII CGCG 1 cut(s) 2342
AciI CCGC 1 cut(s) 149
AclWI GGATC 2 cut(s) 1715, 2083
AcsI RAATTY 7 cut(s) 239, 328, 683, 1334, 1561, 1894, 2579
AcuI CTGAAG 2 cut(s) 471, 1862
AcvI CACGTG 1 cut(s) 1600
AcyI GRCGYC 3 cut(s) 135, 473, 2026
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 148, 615
AflIII ACRYGT 3 cut(s) 205, 1599, 2542
AgeI ACCGGT 1 cut(s) 572
AjnI CCWGG 1 cut(s) 141
AleI CACNNNNGTG 1 cut(s) 1575
AluBI AGCT 9 cut(s) 106, 256, 720, 974, 1148, 1795, 1867, 1888, 1903
AluI AGCT 9 cut(s) 106, 256, 720, 974, 1148, 1795, 1867, 1888, 1903
Alw21I GWGCWC 3 cut(s) 273, 1553, 1765
Alw26I GTCTC 5 cut(s) 107, 189, 488, 563, 715
Alw44I GTGCAC 1 cut(s) 1761
AlwI GGATC 2 cut(s) 1715, 2083
AlwNI CAGNNNCTG 2 cut(s) 106, 2221
Ama87I CYCGRG 1 cut(s) 2387
AoxI GGCC 2 cut(s) 1420, 2528
ApaLI GTGCAC 1 cut(s) 1761
ApeKI GCWGC 7 cut(s) 56, 106, 755, 776, 1250, 1900, 2431
ApoI RAATTY 7 cut(s) 239, 328, 683, 1334, 1561, 1894, 2579
AsiGI ACCGGT 1 cut(s) 572
AspLEI GCGC 1 cut(s) 2344
AspS9I GGNCC 2 cut(s) 1677, 2529
AsuC2I CCSGG 1 cut(s) 1646
AsuHPI GGTGA 2 cut(s) 548, 2023
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 2387
AvaII GGWCC 1 cut(s) 1677
BaeGI GKGCMC 1 cut(s) 1765
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 88
BbrPI CACGTG 1 cut(s) 1600
BbsI GAAGAC 3 cut(s) 1007, 1487, 2240
Bbv12I GWGCWC 3 cut(s) 273, 1553, 1765
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 7 cut(s) 43, 93, 742, 763, 1237, 1912, 2443
BccI CCATC 5 cut(s) 398, 527, 617, 1288, 1360
BceAI ACGGC 2 cut(s) 168, 2036
BcgI CGANNNNNNTGC 2 cut(s) 2320, 2354
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 1 cut(s) 1962
BcnI CCSGG 1 cut(s) 1646
BcoDI GTCTC 5 cut(s) 107, 189, 488, 563, 715
BfmI CTRYAG 2 cut(s) 107, 2313
BfuI GTATCC 1 cut(s) 856
BglI GCCNNNNNGGC 1 cut(s) 2299
BglII AGATCT 1 cut(s) 790
BisI GCNGC 7 cut(s) 57, 107, 756, 777, 1251, 1901, 2432
BlsI GCNGC 7 cut(s) 58, 108, 757, 778, 1252, 1902, 2433
Bme1390I CCNGG 2 cut(s) 143, 1646
Bme18I GGWCC 1 cut(s) 1677
BmeT110I CYCGRG 1 cut(s) 2387
BmgT120I GGNCC 2 cut(s) 1677, 2529
BmiI GGNNCC 3 cut(s) 85, 465, 928
BmrFI CCNGG 2 cut(s) 143, 1646
BmrI ACTGGG 1 cut(s) 1866
BmsI GCATC 7 cut(s) 136, 208, 1009, 1746, 2247, 2430, 2492
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1866
BpiI GAAGAC 3 cut(s) 1007, 1487, 2240
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 2 cut(s) 125, 1643
Bpu10I CCTNAGC 2 cut(s) 222, 1138
BpuEI CTTGAG 6 cut(s) 113, 1367, 1411, 1762, 1808, 1952
BpuMI CCSGG 1 cut(s) 1646
Bsa29I ATCGAT 1 cut(s) 2595
BsaAI YACGTR 1 cut(s) 1600
BsaHI GRCGYC 3 cut(s) 135, 473, 2026
BsaI GGTCTC 3 cut(s) 107, 189, 563
BsaJI CCNNGG 3 cut(s) 742, 1323, 2266
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 615
Bse118I RCCGGY 2 cut(s) 14, 572
Bse1I ACTGG 3 cut(s) 1196, 1660, 1872
Bse3DI GCAATG 3 cut(s) 18, 967, 2443
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 2595
BseDI CCNNGG 3 cut(s) 742, 1323, 2266
BseGI GGATG 4 cut(s) 151, 232, 1371, 2445
BseLI CCNNNNNNNGG 2 cut(s) 148, 615
BseMI GCAATG 3 cut(s) 18, 967, 2443
BseMII CTCAG 4 cut(s) 15, 213, 214, 2213
BseNI ACTGG 3 cut(s) 1196, 1660, 1872
BseRI GAGGAG 4 cut(s) 1085, 1143, 1805, 1949
BseSI GKGCMC 1 cut(s) 1765
BseXI GCAGC 7 cut(s) 43, 93, 742, 763, 1237, 1912, 2443
BsgI GTGCAG 2 cut(s) 185, 1236
Bsh1236I CGCG 1 cut(s) 2342
BshFI GGCC 2 cut(s) 1422, 2530
BshNI GGYRCC 1 cut(s) 463
BshTI ACCGGT 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 2595
BsiHKAI GWGCWC 3 cut(s) 273, 1553, 1765
BsiHKCI CYCGRG 1 cut(s) 2387
BsiSI CCGG 5 cut(s) 15, 461, 573, 1594, 1646
BslFI GGGAC 2 cut(s) 1340, 2531
BslI CCNNNNNNNGG 2 cut(s) 148, 615
BsmAI GTCTC 5 cut(s) 107, 189, 488, 563, 715
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 2 cut(s) 1340, 2531
BsmI GAATGC 1 cut(s) 1649
BsnI GGCC 2 cut(s) 1422, 2530
Bso31I GGTCTC 3 cut(s) 107, 189, 563
BsoBI CYCGRG 1 cut(s) 2387
Bsp1286I GDGCHC 4 cut(s) 88, 273, 1553, 1765
Bsp143I GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
Bsp19I CCATGG 1 cut(s) 2266
BspACI CCGC 1 cut(s) 149
BspANI GGCC 2 cut(s) 1422, 2530
BspCNI CTCAG 4 cut(s) 16, 213, 214, 2214
BspDI ATCGAT 1 cut(s) 2595
BspFNI CGCG 1 cut(s) 2342
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 3 cut(s) 85, 465, 928
BspMAI CTGCAG 2 cut(s) 111, 2317
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 1715, 2083
BspT107I GGYRCC 1 cut(s) 463
BspTNI GGTCTC 3 cut(s) 107, 189, 563
BsrDI GCAATG 3 cut(s) 18, 967, 2443
BsrFI RCCGGY 2 cut(s) 14, 572
BsrI ACTGG 3 cut(s) 1196, 1660, 1872
BssAI RCCGGY 2 cut(s) 14, 572
BssECI CCNNGG 3 cut(s) 742, 1323, 2266
BssMI GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
BssNI GRCGYC 3 cut(s) 135, 473, 2026
BssT1I CCWWGG 2 cut(s) 1323, 2266
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 5 cut(s) 1697, 1919, 2118, 2132, 2217
Bst6I CTCTTC 4 cut(s) 283, 523, 1626, 2079
BstACI GRCGYC 3 cut(s) 135, 473, 2026
BstAPI GCANNNNNTGC 2 cut(s) 106, 2440
BstBAI YACGTR 1 cut(s) 1600
BstC8I GCNNGC 4 cut(s) 45, 645, 1761, 1886
BstDEI CTNAG 6 cut(s) 24, 200, 222, 1138, 1970, 2222
BstDSI CCRYGG 2 cut(s) 742, 2266
BstF5I GGATG 4 cut(s) 151, 232, 1371, 2445
BstFNI CGCG 1 cut(s) 2342
BstHHI GCGC 1 cut(s) 2344
BstKTI GATC 7 cut(s) 793, 1710, 1965, 2091, 2196, 2255, 2538
BstMAI GTCTC 5 cut(s) 107, 189, 488, 563, 715
BstMBI GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 106, 1419, 2299, 2440
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 4 cut(s) 209, 1000, 1737, 2546
BstSCI CCNGG 2 cut(s) 141, 1644
BstSFI CTRYAG 2 cut(s) 107, 2313
BstSLI GKGCMC 1 cut(s) 1765
BstUI CGCG 1 cut(s) 2342
BstV1I GCAGC 7 cut(s) 43, 93, 742, 763, 1237, 1912, 2443
BstV2I GAAGAC 3 cut(s) 1007, 1487, 2240
BstX2I RGATCY 3 cut(s) 790, 1707, 2088
BstYI RGATCY 3 cut(s) 790, 1707, 2088
Bsu15I ATCGAT 1 cut(s) 2595
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 2 cut(s) 1422, 2530
BsuTUI ATCGAT 1 cut(s) 2595
BtgI CCRYGG 2 cut(s) 742, 2266
BtsCI GGATG 4 cut(s) 151, 232, 1371, 2445
BtsI GCAGTG 3 cut(s) 1274, 2351, 2451
BtsIMutI CAGTG 7 cut(s) 660, 1189, 1274, 1702, 2128, 2351, 2451
Cac8I GCNNGC 4 cut(s) 45, 645, 1761, 1886
CaiI CAGNNNCTG 2 cut(s) 106, 2221
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 1 cut(s) 2344
Cfr10I RCCGGY 2 cut(s) 14, 572
Cfr13I GGNCC 2 cut(s) 1677, 2529
ClaI ATCGAT 1 cut(s) 2595
CseI GACGC 3 cut(s) 378, 697, 2034
Csp6I GTAC 1 cut(s) 305
CspAI ACCGGT 1 cut(s) 572
CspCI CAANNNNNGTGG 5 cut(s) 36, 1515, 1550, 2142, 2177
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 6 cut(s) 24, 200, 222, 1138, 1970, 2222
DpnI GATC 7 cut(s) 792, 1709, 1964, 2090, 2195, 2254, 2537
DpnII GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
DraI TTTAAA 1 cut(s) 1008
Eam1104I CTCTTC 4 cut(s) 283, 523, 1626, 2079
EarI CTCTTC 4 cut(s) 283, 523, 1626, 2079
Eco130I CCWWGG 2 cut(s) 1323, 2266
Eco147I AGGCCT 1 cut(s) 1422
Eco24I GRGCYC 1 cut(s) 88
Eco31I GGTCTC 3 cut(s) 107, 189, 563
Eco32I GATATC 3 cut(s) 727, 1832, 2593
Eco47I GGWCC 1 cut(s) 1677
Eco57I CTGAAG 2 cut(s) 471, 1862
Eco72I CACGTG 1 cut(s) 1600
Eco88I CYCGRG 1 cut(s) 2387
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 3 cut(s) 727, 1832, 2593
EcoT14I CCWWGG 2 cut(s) 1323, 2266
EcoT22I ATGCAT 4 cut(s) 1002, 1057, 1739, 2445
EcoT38I GRGCYC 1 cut(s) 88
ErhI CCWWGG 2 cut(s) 1323, 2266
Esp3I CGTCTC 1 cut(s) 715
FaqI GGGAC 2 cut(s) 1340, 2531
FbaI TGATCA 1 cut(s) 1962
Fnu4HI GCNGC 7 cut(s) 57, 107, 756, 777, 1251, 1901, 2432
FokI GGATG 4 cut(s) 158, 239, 1378, 2452
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 7 cut(s) 57, 107, 756, 777, 1251, 1901, 2432
GlaI GCGC 1 cut(s) 2343
GluI GCNGC 7 cut(s) 57, 107, 756, 777, 1251, 1901, 2432
GsuI CTGGAG 2 cut(s) 125, 1643
HaeIII GGCC 2 cut(s) 1422, 2530
HapII CCGG 5 cut(s) 15, 461, 573, 1594, 1646
HgaI GACGC 3 cut(s) 378, 697, 2034
HhaI GCGC 1 cut(s) 2344
Hin1I GRCGYC 3 cut(s) 135, 473, 2026
Hin6I GCGC 1 cut(s) 2342
HinP1I GCGC 1 cut(s) 2342
HincII GTYRAC 1 cut(s) 2114
HindII GTYRAC 1 cut(s) 2114
HpaII CCGG 5 cut(s) 15, 461, 573, 1594, 1646
HphI GGTGA 2 cut(s) 548, 2023
Hpy166II GTNNAC 4 cut(s) 307, 1313, 1763, 2114
Hpy188I TCNGA 6 cut(s) 203, 260, 715, 2010, 2278, 2572
Hpy8I GTNNAC 4 cut(s) 307, 1313, 1763, 2114
Hpy99I CGWCG 2 cut(s) 478, 2567
HpyAV CCTTC 5 cut(s) 483, 1058, 1837, 2117, 2228
HpyCH4III ACNGT 5 cut(s) 1697, 1919, 2118, 2132, 2217
HpyCH4IV ACGT 5 cut(s) 30, 135, 382, 473, 1599
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 106, 1419, 2299, 2440
HpyF3I CTNAG 6 cut(s) 24, 200, 222, 1138, 1970, 2222
HpySE526I ACGT 5 cut(s) 30, 135, 382, 473, 1599
Hsp92I GRCGYC 3 cut(s) 135, 473, 2026
HspAI GCGC 1 cut(s) 2342
Ksp22I TGATCA 1 cut(s) 1962
Kzo9I GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
LmnI GCTCC 6 cut(s) 83, 261, 926, 1180, 1556, 1792
Lsp1109I GCAGC 7 cut(s) 43, 93, 742, 763, 1237, 1912, 2443
LweI GCATC 7 cut(s) 136, 208, 1009, 1746, 2247, 2430, 2492
MaeII ACGT 5 cut(s) 30, 135, 382, 473, 1599
MaeIII GTNAC 8 cut(s) 949, 1074, 1194, 1265, 1568, 1811, 1938, 2217
MalI GATC 7 cut(s) 792, 1709, 1964, 2090, 2195, 2254, 2537
MboI GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
MfeI CAATTG 1 cut(s) 2256
MflI RGATCY 3 cut(s) 790, 1707, 2088
MhlI GDGCHC 4 cut(s) 88, 273, 1553, 1765
MlyI GAGTC 9 cut(s) 255, 300, 494, 878, 1082, 1168, 2153, 2318, 2561
MmeI TCCRAC 5 cut(s) 283, 305, 505, 828, 904
Mph1103I ATGCAT 4 cut(s) 1002, 1057, 1739, 2445
MslI CAYNNNNRTG 1 cut(s) 1575
MspI CCGG 5 cut(s) 15, 461, 573, 1594, 1646
MspR9I CCNGG 2 cut(s) 143, 1646
MunI CAATTG 1 cut(s) 2256
Mva1269I GAATGC 1 cut(s) 1649
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 2342
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 106, 1419, 2299, 2440
NciI CCSGG 1 cut(s) 1646
NcoI CCATGG 1 cut(s) 2266
NdeII GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 3 cut(s) 85, 465, 928
NmuCI GTSAC 2 cut(s) 1074, 1568
NsiI ATGCAT 4 cut(s) 1002, 1057, 1739, 2445
NspI RCATGY 4 cut(s) 209, 1000, 1737, 2546
OliI CACNNNNGTG 1 cut(s) 1575
PaeR7I CTCGAG 1 cut(s) 2387
PagI TCATGA 1 cut(s) 1021
PceI AGGCCT 1 cut(s) 1422
PciI ACATGT 2 cut(s) 205, 2542
PctI GAATGC 1 cut(s) 1649
PfeI GAWTC 7 cut(s) 169, 965, 1028, 1641, 1746, 1819, 2065
PflFI GACNNNGTC 1 cut(s) 854
PfoI TCCNGGA 1 cut(s) 141
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 7 cut(s) 58, 108, 757, 778, 1252, 1902, 2433
PleI GAGTC 9 cut(s) 255, 299, 494, 877, 1081, 1167, 2152, 2317, 2561
PmaCI CACGTG 1 cut(s) 1600
PmlI CACGTG 1 cut(s) 1600
PpsI GAGTC 9 cut(s) 255, 299, 494, 877, 1081, 1167, 2152, 2317, 2561
Ppu21I YACGTR 1 cut(s) 1600
PscI ACATGT 2 cut(s) 205, 2542
Psp6I CCWGG 1 cut(s) 141
PspCI CACGTG 1 cut(s) 1600
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 3 cut(s) 85, 465, 928
PspPI GGNCC 2 cut(s) 1677, 2529
PstI CTGCAG 2 cut(s) 111, 2317
PstNI CAGNNNCTG 2 cut(s) 106, 2221
PsuI RGATCY 3 cut(s) 790, 1707, 2088
PsyI GACNNNGTC 1 cut(s) 854
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 1575
SatI GCNGC 7 cut(s) 57, 107, 756, 777, 1251, 1901, 2432
Sau3AI GATC 7 cut(s) 790, 1707, 1962, 2088, 2193, 2252, 2535
Sau96I GGNCC 2 cut(s) 1677, 2529
SchI GAGTC 9 cut(s) 255, 300, 494, 878, 1082, 1168, 2153, 2318, 2561
ScrFI CCNGG 2 cut(s) 143, 1646
SduI GDGCHC 4 cut(s) 88, 273, 1553, 1765
SfaNI GCATC 7 cut(s) 136, 208, 1009, 1746, 2247, 2430, 2492
SfcI CTRYAG 2 cut(s) 107, 2313
Sfr274I CTCGAG 1 cut(s) 2387
SinI GGWCC 1 cut(s) 1677
SlaI CTCGAG 1 cut(s) 2387
SmiMI CAYNNNNRTG 1 cut(s) 1575
SmlI CTYRAG 7 cut(s) 92, 1382, 1390, 1741, 1787, 1931, 2387
SmoI CTYRAG 7 cut(s) 92, 1382, 1390, 1741, 1787, 1931, 2387
SseBI AGGCCT 1 cut(s) 1422
SsiI CCGC 1 cut(s) 149
SspI AATATT 1 cut(s) 1721
StuI AGGCCT 1 cut(s) 1422
StyD4I CCNGG 2 cut(s) 141, 1644
StyI CCWWGG 2 cut(s) 1323, 2266
TaaI ACNGT 5 cut(s) 1697, 1919, 2118, 2132, 2217
TaiI ACGT 5 cut(s) 33, 138, 385, 476, 1602
TaqI TCGA 9 cut(s) 264, 476, 588, 1172, 1664, 2251, 2388, 2562, 2595
TaqII GACCGA 2 cut(s) 545, 2186
TfiI GAWTC 7 cut(s) 169, 965, 1028, 1641, 1746, 1819, 2065
TscAI CASTG 7 cut(s) 660, 1196, 1281, 1702, 2135, 2351, 2458
TseFI GTSAC 2 cut(s) 1074, 1568
TseI GCWGC 7 cut(s) 56, 106, 755, 776, 1250, 1900, 2431
Tsp45I GTSAC 2 cut(s) 1074, 1568
TspDTI ATGAA 9 cut(s) 812, 978, 1077, 1227, 1347, 1416, 1642, 1811, 2592
TspGWI ACGGA 3 cut(s) 322, 731, 1690
TspRI CASTG 7 cut(s) 660, 1196, 1281, 1702, 2135, 2351, 2458
Tth111I GACNNNGTC 1 cut(s) 854
VneI GTGCAC 1 cut(s) 1761
VpaK11BI GGWCC 1 cut(s) 1677
XapI RAATTY 7 cut(s) 239, 328, 683, 1334, 1561, 1894, 2579
XbaI TCTAGA 2 cut(s) 1625, 1690
XceI RCATGY 4 cut(s) 209, 1000, 1737, 2546
XhoI CTCGAG 1 cut(s) 2387
ZraI GACGTC 2 cut(s) 136, 474
Zsp2I ATGCAT 4 cut(s) 1002, 1057, 1739, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.