Rw7G002430

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000159
Physical Location & Seq
Forward (+)
1970462 .. 1976057
5596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002430.1

Sequence Viewer

Length: 2754 bp
ATGGGCTCAAAGTCCAATGGTAGTACTAGCTATTATCTAGAGAAACCATCGTTGTTGCGAGGCCTGAAGAGTGAGGTTAAGGGGGACAGTACCGTCTTTCAGCAAGCGGAGTATTTGGTTCATTTGGGGAACTTGAAATTTTGTCTTGCCCAAACTATCATGGATGACTTGACTACAGGGCGTCAACCTCTATGGATCACCACATTCCGGGTTATCAATGAAGAAGGAGGAGGAAGGTATATCAAGACATTACATTCAACTGTTCGTGATGTGAACATAAAGTGTTTTGGTAAGGGTTTTGGTAGATTCACGGTTGATTTTAGCTTCATAGTTGGGGAGGATTTTGAAGTTTTGAACCAAAAGAAAGAGGGAGTACGTGTCGATATGATGGAGGAGCAGCCGAAAGAAGAAACTGCACTGATTTCTGATGAAATGATGGAGGACAGAGGTGTTTCAGTAAATGAAGAAGAAACAAATTCCCAATGCGATTCAGAAAATGAAGAAGAAACAAAATGTGAAGGTGAGTCAGAAAATGAAGAAACAAAATGCGAAGGTGATTCAGAAAATGAAGAAAGAGAAGACAAGGAGAAGTCTGTTAATGGCGTTAATGCTTGTCCATTGGCTAAGGAAAAGGGGACAGCCAAAAAGAGAGACAAAAGATTGAGTATTTTTCACAGGAGCCTTAAAGCCAAGTCTGGTCACAAGAAGCGTAAATGGAAGAATGTTTCATCAAGCGTTGCAGAGAGCGTCTTGGGTAGGCTAGCCTCTTATGCTGCCCAAGAAATCTGCTTGGCATGGGGTGCCCAACTTGAGTTGCAAAAGCTCAAACAGACCTTGTTAGACATCAAAGACGTCCTCCAGGATGCGGAGGAAAAGCAGGTGAACAATCCTGTAATCAGCCGTTGGTTAGGAGACCTTAAAGATGTTTGCAACGATGCCGAGGATGTATTGGACGAATTTGAGTTCCGAAAGCTCCGACTCGAAGTGCTCATCAACAACCACTGGAGAGTCAAAGGAGAGGTACACCAATTCTTCTCTCGTTGGAATTCCGTTGTGTTTAACTTCAAATTGGGTCATAAATTAAGACGCATTAGAGAACGTCTAGTTCAAATTGATAATGACGGAAAGACCAAGTTTTCTCTCGTTATTAAAGTTGTTCAACAAACTGAAGTTTGTCCGGCACCCTTGAGGCCGACTGGTAATAAAAAGGAGACAGACTCTTTACTTGAAGGTGTTGTTGGAAGAGATGGTGATAGGGAAAAAATTATCCATCTCTTTGATGACACCCTTTCCTCCAGTGTTGATAATGTTTCAGTTATTTCCATCTTTGGGTTAGGAGGGTTGGGAAAAACTACACTTGCTAAATCGGTGTATAATGATGCTATGGTAGAGAACAAATTTGATATGAGAATGTGGGTATGCGTCTCAGAAAACTTTGATATCCATACATTAGTTCGTGGCATTATTGCTGCAACTAAACAAGAATGTGCTGTTGAAAGTTTAGATCTTATGAAAAACGGGTTGCAGGGTATTTTGAAAGATAAAAAGTTTTTGTTAGTGTTGGACGATGTATGGGATAAAGAGTCGGTGGGAGTTACAAATCAGAAATGGAGAGAGTTAAAAACTTTGTTAGATGTTGGAGCCAGAGGAAGTAAAATCATCGTTACAACACGTAATGAATCAGTAGCTTCACTTGTACACCCTATACATAAGCATCAGTTAAGAGGTCTTTCTCAAGAGGATTCCATGACTTTGTTTAAAAACAATGCATTTAACGAAGGAGAGGAGTCACACTATCAACACTTGATAAAAATTGGAGAGGACATTGTGCATAAGTGTGGAGGGGTTCCCTTGGCACTAGCTACTCTAGGGAGTCTGCTTCGCTTGAAAAGGGAGCCACAAGACTGGGTACAAACTACTGTGCGGTTCAAAATCCATGATTTAGTGCACGACTTGGCAATATCAGTGGCACAGATACAGTGCTCCACAGTTAATTTTCGTCCCTCTAGCGCTTTAGAAAAAGTCCGGCACGTGTCAATATCGGGAAAAGACTTGTCTGGAGAAAAGGCAGGAGTCCCGGCATTCCTTCTCCAGTCGGAGAAACTGCGGACCATTCTAAATGTAGATAGTGAAGCTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCGCTAGATCTGCGTCGTTCTACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAACCTGGATTTTAATGAGAAGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTGGTGGTTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAATCTCAGATATTTGTATATAACTACACAGCAGATGTATTTTCCGAAAGGAGTATTTAGACGCCTCACCTTACTTCAATCCTTGTTCATTGTGAATTGTGCGAATCTGAAATCTCTGGGAGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTGGATCGTTTACTGTGAAAATCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCATTCCATACTTTGGCGATCGTTGATTGTGTGGATTTGAAGAGTTGTAAAAAGAGCATGCGGCCACCGGAGGTGATTCAGAATGAATTAGCAGTGTTGCTGTGCAAGTACCGTACTGCATTTGTTTGGGTCCTGAACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

917

Amino Acids

103.92

Weight (kDa)

7.04

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 247 - 332 2.6e-22 Rx N-terminal domain
NB-ARC PF00931 428 - 593 7.5e-36 NB-ARC domain
LRR_14 PF23598 724 - 898 1.3e-23 Leucine-rich repeat region
LRR_13 PF23286 738 - 884 1.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 868
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 855
Acc36I ACCTGC 1 cut(s) 868
AccB1I GGYRCC 2 cut(s) 800, 1180
AccB7I CCANNNNNTGG 1 cut(s) 2623
AciI CCGC 5 cut(s) 107, 866, 1924, 2107, 2671
AclWI GGATC 4 cut(s) 203, 2147, 2518, 2564
AcoI YGGCCR 1 cut(s) 2672
AcsI RAATTY 6 cut(s) 137, 475, 956, 1045, 1397, 2326
AcuI CTGAAG 2 cut(s) 86, 1188
AcvI CACGTG 1 cut(s) 2032
AcyI GRCGYC 3 cut(s) 181, 852, 2458
AfaI GTAC 8 cut(s) 25, 91, 375, 1023, 1698, 1911, 2720, 2725
AfeI AGCGCT 1 cut(s) 2011
AfiI CCNNNNNNNGG 4 cut(s) 207, 865, 1329, 2623
AflII CTTAAG 1 cut(s) 2338
AflIII ACRYGT 3 cut(s) 376, 1670, 2031
AjnI CCWGG 2 cut(s) 858, 2271
AjuI GAANNNNNNNTTGG 2 cut(s) 1221, 1253
Alw21I GWGCWC 3 cut(s) 990, 1950, 1985
Alw26I GTCTC 4 cut(s) 645, 906, 1205, 1429
Alw44I GTGCAC 1 cut(s) 1946
AlwI GGATC 4 cut(s) 203, 2147, 2518, 2564
Aor51HI AGCGCT 1 cut(s) 2011
AoxI GGCC 3 cut(s) 61, 1190, 2672
ApaLI GTGCAC 1 cut(s) 1946
ApeKI GCWGC 3 cut(s) 397, 773, 1469
ApoI RAATTY 6 cut(s) 137, 475, 956, 1045, 1397, 2326
AspLEI GCGC 2 cut(s) 2012, 2197
AspS9I GGNCC 2 cut(s) 2109, 2740
AsuC2I CCSGG 2 cut(s) 209, 2078
AsuHPI GGTGA 7 cut(s) 190, 533, 566, 892, 1262, 2455, 2695
AsuNHI GCTAGC 1 cut(s) 760
AvaII GGWCC 2 cut(s) 2109, 2740
BaeGI GKGCMC 2 cut(s) 805, 1950
BanI GGYRCC 2 cut(s) 800, 1180
BanII GRGCYC 1 cut(s) 8
BbrPI CACGTG 1 cut(s) 2032
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 3 cut(s) 990, 1950, 1985
BbvI GCAGC 3 cut(s) 409, 760, 1456
BccI CCATC 6 cut(s) 55, 382, 430, 1241, 1278, 1331
BceAI ACGGC 1 cut(s) 885
BciT130I CCWGG 2 cut(s) 860, 2273
BclI TGATCA 1 cut(s) 2394
BcnI CCSGG 2 cut(s) 209, 2078
BcoDI GTCTC 4 cut(s) 645, 906, 1205, 1429
BfaI CTAG 8 cut(s) 27, 38, 761, 1103, 1859, 1868, 2007, 2198
BfmI CTRYAG 1 cut(s) 174
BfoI RGCGCY 1 cut(s) 2013
BfrI CTTAAG 1 cut(s) 2338
BfuAI ACCTGC 1 cut(s) 868
BglII AGATCT 2 cut(s) 1504, 2200
BisI GCNGC 4 cut(s) 398, 774, 1470, 2672
BlsI GCNGC 4 cut(s) 399, 775, 1471, 2673
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 4 cut(s) 209, 860, 2078, 2273
Bme18I GGWCC 2 cut(s) 2109, 2740
BmgT120I GGNCC 2 cut(s) 2109, 2740
BmiI GGNNCC 8 cut(s) 680, 802, 1182, 1642, 1848, 1896, 2390, 2741
BmrFI CCNGG 4 cut(s) 209, 860, 2078, 2273
BmrI ACTGGG 2 cut(s) 1915, 2298
BmsI GCATC 5 cut(s) 853, 925, 1369, 1723, 2178
BmtI GCTAGC 1 cut(s) 764
BmuI ACTGGG 2 cut(s) 1915, 2298
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 1202, 1234
BpmI CTGGAG 6 cut(s) 842, 1024, 1279, 2075, 2079, 2597
Bpu10I CCTNAGC 1 cut(s) 624
BpuEI CTTGAG 6 cut(s) 830, 1207, 1719, 2194, 2240, 2384
BpuMI CCSGG 2 cut(s) 209, 2078
BsaAI YACGTR 3 cut(s) 377, 1673, 2032
BsaHI GRCGYC 3 cut(s) 181, 852, 2458
BsaI GGTCTC 1 cut(s) 906
BsaJI CCNNGG 3 cut(s) 939, 1851, 2344
BsaWI WCCGGW 1 cut(s) 2677
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 865, 1329, 2623
Bse1I ACTGG 6 cut(s) 1007, 1201, 1296, 1910, 2092, 2304
BseBI CCWGG 2 cut(s) 860, 2273
BseDI CCNNGG 3 cut(s) 939, 1851, 2344
BseGI GGATG 3 cut(s) 169, 868, 949
BseLI CCNNNNNNNGG 4 cut(s) 207, 865, 1329, 2623
BseMII CTCAG 2 cut(s) 1440, 2416
BseNI ACTGG 6 cut(s) 1007, 1201, 1296, 1910, 2092, 2304
BseRI GAGGAG 5 cut(s) 243, 407, 1799, 2237, 2381
BseSI GKGCMC 2 cut(s) 805, 1950
BseXI GCAGC 3 cut(s) 409, 760, 1456
BseYI CCCAGC 1 cut(s) 2135
BsgI GTGCAG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 2631
BshFI GGCC 3 cut(s) 63, 1192, 2674
BshNI GGYRCC 2 cut(s) 800, 1180
BsiEI CGRYCG 1 cut(s) 2631
BsiHKAI GWGCWC 3 cut(s) 990, 1950, 1985
BsiSI CCGG 5 cut(s) 208, 1178, 2026, 2078, 2678
BslFI GGGAC 4 cut(s) 98, 649, 1986, 2060
BslI CCNNNNNNNGG 4 cut(s) 207, 865, 1329, 2623
BsmAI GTCTC 4 cut(s) 645, 906, 1205, 1429
BsmBI CGTCTC 1 cut(s) 1429
BsmFI GGGAC 4 cut(s) 98, 649, 1986, 2060
BsmI GAATGC 2 cut(s) 2081, 2612
BsnI GGCC 3 cut(s) 63, 1192, 2674
Bso31I GGTCTC 1 cut(s) 906
Bsp1286I GDGCHC 5 cut(s) 8, 805, 990, 1950, 1985
Bsp1407I TGTACA 1 cut(s) 1696
Bsp143I GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
BspACI CCGC 5 cut(s) 107, 866, 1924, 2107, 2671
BspANI GGCC 3 cut(s) 63, 1192, 2674
BspCNI CTCAG 2 cut(s) 1439, 2415
BspLI GGNNCC 8 cut(s) 680, 802, 1182, 1642, 1848, 1896, 2390, 2741
BspMI ACCTGC 1 cut(s) 868
BspOI GCTAGC 1 cut(s) 764
BspPI GGATC 4 cut(s) 203, 2147, 2518, 2564
BspT107I GGYRCC 2 cut(s) 800, 1180
BspTI CTTAAG 1 cut(s) 2338
BspTNI GGTCTC 1 cut(s) 906
BsrGI TGTACA 1 cut(s) 1696
BsrI ACTGG 6 cut(s) 1007, 1201, 1296, 1910, 2092, 2304
BssECI CCNNGG 3 cut(s) 939, 1851, 2344
BssMI GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
BssNI GRCGYC 3 cut(s) 181, 852, 2458
BssT1I CCWWGG 2 cut(s) 1851, 2344
Bst2UI CCWGG 2 cut(s) 860, 2273
Bst6I CTCTTC 4 cut(s) 62, 1237, 2514, 2645
BstACI GRCGYC 3 cut(s) 181, 852, 2458
BstAFI CTTAAG 1 cut(s) 2338
BstAPI GCANNNNNTGC 1 cut(s) 800
BstAUI TGTACA 1 cut(s) 1696
BstBAI YACGTR 3 cut(s) 377, 1673, 2032
BstC8I GCNNGC 5 cut(s) 105, 762, 2193, 2318, 2669
BstDEI CTNAG 3 cut(s) 624, 1426, 2402
BstF5I GGATG 3 cut(s) 169, 868, 949
BstH2I RGCGCY 1 cut(s) 2013
BstHHI GCGC 2 cut(s) 2012, 2197
BstKTI GATC 8 cut(s) 198, 1507, 2142, 2203, 2397, 2526, 2559, 2631
BstMAI GTCTC 4 cut(s) 645, 906, 1205, 1429
BstMBI GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
BstMCI CGRYCG 1 cut(s) 2631
BstMWI GCNNNNNNNGC 3 cut(s) 770, 800, 2203
BstNI CCWGG 2 cut(s) 860, 2273
BstNSI RCATGY 2 cut(s) 2169, 2671
BstSCI CCNGG 4 cut(s) 207, 858, 2076, 2271
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 2 cut(s) 805, 1950
BstV1I GCAGC 3 cut(s) 409, 760, 1456
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 4 cut(s) 1504, 2139, 2200, 2523
BstXI CCANNNNNNTGG 1 cut(s) 1905
BstYI RGATCY 4 cut(s) 1504, 2139, 2200, 2523
BsuRI GGCC 3 cut(s) 63, 1192, 2674
BtsCI GGATG 3 cut(s) 169, 868, 949
BtsI GCAGTG 1 cut(s) 2709
BtsIMutI CAGTG 6 cut(s) 416, 1000, 1303, 1971, 1985, 2709
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 5 cut(s) 105, 762, 2193, 2318, 2669
CfoI GCGC 2 cut(s) 2012, 2197
Cfr13I GGNCC 2 cut(s) 2109, 2740
CseI GACGC 6 cut(s) 170, 736, 1095, 1411, 2195, 2466
Csp6I GTAC 8 cut(s) 24, 90, 374, 1022, 1697, 1910, 2719, 2724
CspCI CAANNNNNGTGG 2 cut(s) 1947, 1982
CviAII CATG 7 cut(s) 160, 795, 1747, 1937, 2166, 2596, 2668
CviQI GTAC 8 cut(s) 24, 90, 374, 1022, 1697, 1910, 2719, 2724
DdeI CTNAG 3 cut(s) 624, 1426, 2402
DpnI GATC 8 cut(s) 197, 1506, 2141, 2202, 2396, 2525, 2558, 2630
DpnII GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
DraI TTTAAA 1 cut(s) 1759
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 2672
Eam1104I CTCTTC 4 cut(s) 62, 1237, 2514, 2645
EarI CTCTTC 4 cut(s) 62, 1237, 2514, 2645
Eco130I CCWWGG 2 cut(s) 1851, 2344
Eco147I AGGCCT 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 906
Eco32I GATATC 2 cut(s) 1441, 2264
Eco47I GGWCC 2 cut(s) 2109, 2740
Eco47III AGCGCT 1 cut(s) 2011
Eco57I CTGAAG 2 cut(s) 86, 1188
Eco72I CACGTG 1 cut(s) 2032
EcoO109I RGGNCCY 1 cut(s) 2740
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 858, 2271
EcoRV GATATC 2 cut(s) 1441, 2264
EcoT14I CCWWGG 2 cut(s) 1851, 2344
EcoT22I ATGCAT 2 cut(s) 1771, 2171
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 2 cut(s) 1851, 2344
Esp3I CGTCTC 1 cut(s) 1429
FaeI CATG 7 cut(s) 163, 798, 1750, 1940, 2169, 2599, 2671
FaqI GGGAC 4 cut(s) 98, 649, 1986, 2060
FatI CATG 7 cut(s) 159, 794, 1746, 1936, 2165, 2595, 2667
FbaI TGATCA 1 cut(s) 2394
Fnu4HI GCNGC 4 cut(s) 398, 774, 1470, 2672
FokI GGATG 3 cut(s) 176, 875, 956
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 398, 774, 1470, 2672
FspBI CTAG 8 cut(s) 27, 38, 761, 1103, 1859, 1868, 2007, 2198
GlaI GCGC 2 cut(s) 2011, 2196
GluI GCNGC 4 cut(s) 398, 774, 1470, 2672
GsaI CCCAGC 1 cut(s) 2139
GsuI CTGGAG 6 cut(s) 842, 1024, 1279, 2075, 2079, 2597
HaeII RGCGCY 1 cut(s) 2013
HaeIII GGCC 3 cut(s) 63, 1192, 2674
HapII CCGG 5 cut(s) 208, 1178, 2026, 2078, 2678
HgaI GACGC 6 cut(s) 170, 736, 1095, 1411, 2195, 2466
HhaI GCGC 2 cut(s) 2012, 2197
Hin1I GRCGYC 3 cut(s) 181, 852, 2458
Hin1II CATG 7 cut(s) 163, 798, 1750, 1940, 2169, 2599, 2671
Hin6I GCGC 2 cut(s) 2010, 2195
HinP1I GCGC 2 cut(s) 2010, 2195
HincII GTYRAC 2 cut(s) 185, 2549
HindII GTYRAC 2 cut(s) 185, 2549
HpaII CCGG 5 cut(s) 208, 1178, 2026, 2078, 2678
HphI GGTGA 7 cut(s) 190, 533, 566, 892, 1262, 2455, 2695
Hpy166II GTNNAC 8 cut(s) 185, 274, 883, 1024, 1699, 1948, 2549, 2563
Hpy8I GTNNAC 8 cut(s) 185, 274, 883, 1024, 1699, 1948, 2549, 2563
Hpy99I CGWCG 1 cut(s) 2211
HpyAV CCTTC 8 cut(s) 218, 228, 512, 545, 1223, 1772, 2096, 2552
HpyCH4IV ACGT 5 cut(s) 376, 852, 1099, 1672, 2031
HpyF10VI GCNNNNNNNGC 3 cut(s) 770, 800, 2203
HpyF3I CTNAG 3 cut(s) 624, 1426, 2402
HpySE526I ACGT 5 cut(s) 376, 852, 1099, 1672, 2031
Hsp92I GRCGYC 3 cut(s) 181, 852, 2458
Hsp92II CATG 7 cut(s) 163, 798, 1750, 1940, 2169, 2599, 2671
HspAI GCGC 2 cut(s) 2010, 2195
Ksp22I TGATCA 1 cut(s) 2394
Kzo9I GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
LmnI GCTCC 7 cut(s) 394, 678, 978, 1640, 1894, 1988, 2224
Lsp1109I GCAGC 3 cut(s) 409, 760, 1456
LweI GCATC 5 cut(s) 853, 925, 1369, 1723, 2178
MaeI CTAG 8 cut(s) 27, 38, 761, 1103, 1859, 1868, 2007, 2198
MaeII ACGT 5 cut(s) 376, 852, 1099, 1672, 2031
MaeIII GTNAC 6 cut(s) 698, 1594, 1663, 1788, 2243, 2370
MalI GATC 8 cut(s) 197, 1506, 2141, 2202, 2396, 2525, 2558, 2630
MboI GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
MflI RGATCY 4 cut(s) 1504, 2139, 2200, 2523
MhlI GDGCHC 5 cut(s) 8, 805, 990, 1950, 1985
MlyI GAGTC 9 cut(s) 533, 972, 1017, 1211, 1592, 1796, 1882, 2082, 2588
MmeI TCCRAC 6 cut(s) 1000, 1022, 1219, 1542, 1618, 2076
Mph1103I ATGCAT 2 cut(s) 1771, 2171
MslI CAYNNNNRTG 1 cut(s) 1836
MspCI CTTAAG 1 cut(s) 2338
MspI CCGG 5 cut(s) 208, 1178, 2026, 2078, 2678
MspR9I CCNGG 4 cut(s) 209, 860, 2078, 2273
Mva1269I GAATGC 2 cut(s) 2081, 2612
MvaI CCWGG 2 cut(s) 860, 2273
MwoI GCNNNNNNNGC 3 cut(s) 770, 800, 2203
NciI CCSGG 2 cut(s) 209, 2078
NdeII GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
NheI GCTAGC 1 cut(s) 760
NlaIII CATG 7 cut(s) 163, 798, 1750, 1940, 2169, 2599, 2671
NlaIV GGNNCC 8 cut(s) 680, 802, 1182, 1642, 1848, 1896, 2390, 2741
NmeAIII GCCGAG 1 cut(s) 964
NmuCI GTSAC 2 cut(s) 698, 1788
NsiI ATGCAT 2 cut(s) 1771, 2171
NspI RCATGY 2 cut(s) 2169, 2671
PaeI GCATGC 1 cut(s) 2671
PaqCI CACCTGC 1 cut(s) 868
PceI AGGCCT 1 cut(s) 63
PctI GAATGC 2 cut(s) 2081, 2612
PfeI GAWTC 9 cut(s) 306, 488, 557, 1679, 1742, 2178, 2251, 2500, 2686
PflMI CCANNNNNTGG 1 cut(s) 2623
PfoI TCCNGGA 1 cut(s) 858
PkrI GCNGC 4 cut(s) 399, 775, 1471, 2673
Ple19I CGATCG 1 cut(s) 2631
PleI GAGTC 9 cut(s) 532, 972, 1016, 1211, 1591, 1795, 1881, 2081, 2587
PmaCI CACGTG 1 cut(s) 2032
PmlI CACGTG 1 cut(s) 2032
PpsI GAGTC 9 cut(s) 532, 972, 1016, 1211, 1591, 1795, 1881, 2081, 2587
Ppu21I YACGTR 3 cut(s) 377, 1673, 2032
PpuMI RGGWCCY 1 cut(s) 2740
Psp5II RGGWCCY 1 cut(s) 2740
Psp6I CCWGG 2 cut(s) 858, 2271
PspCI CACGTG 1 cut(s) 2032
PspFI CCCAGC 1 cut(s) 2135
PspGI CCWGG 2 cut(s) 858, 2271
PspN4I GGNNCC 8 cut(s) 680, 802, 1182, 1642, 1848, 1896, 2390, 2741
PspPI GGNCC 2 cut(s) 2109, 2740
PspPPI RGGWCCY 1 cut(s) 2740
PsuI RGATCY 4 cut(s) 1504, 2139, 2200, 2523
PvuI CGATCG 1 cut(s) 2631
RsaI GTAC 8 cut(s) 25, 91, 375, 1023, 1698, 1911, 2720, 2725
RsaNI GTAC 8 cut(s) 24, 90, 374, 1022, 1697, 1910, 2719, 2724
RseI CAYNNNNRTG 1 cut(s) 1836
SatI GCNGC 4 cut(s) 398, 774, 1470, 2672
Sau3AI GATC 8 cut(s) 195, 1504, 2139, 2200, 2394, 2523, 2556, 2628
Sau96I GGNCC 2 cut(s) 2109, 2740
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 9 cut(s) 533, 972, 1017, 1211, 1592, 1796, 1882, 2082, 2588
ScrFI CCNGG 4 cut(s) 209, 860, 2078, 2273
SduI GDGCHC 5 cut(s) 8, 805, 990, 1950, 1985
SfaNI GCATC 5 cut(s) 853, 925, 1369, 1723, 2178
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 2 cut(s) 2109, 2740
SmiMI CAYNNNNRTG 1 cut(s) 1836
SmlI CTYRAG 7 cut(s) 809, 1186, 1734, 2173, 2219, 2338, 2363
SmoI CTYRAG 7 cut(s) 809, 1186, 1734, 2173, 2219, 2338, 2363
SphI GCATGC 1 cut(s) 2671
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 5 cut(s) 107, 866, 1924, 2107, 2671
SspI AATATT 1 cut(s) 2153
SspMI CTAG 8 cut(s) 27, 38, 761, 1103, 1859, 1868, 2007, 2198
StuI AGGCCT 1 cut(s) 63
StyD4I CCNGG 4 cut(s) 207, 858, 2076, 2271
StyI CCWWGG 2 cut(s) 1851, 2344
TaiI ACGT 5 cut(s) 379, 855, 1102, 1675, 2034
TaqI TCGA 2 cut(s) 381, 981
TatI WGTACW 2 cut(s) 23, 1696
TauI GCSGC 1 cut(s) 2674
TfiI GAWTC 9 cut(s) 306, 488, 557, 1679, 1742, 2178, 2251, 2500, 2686
TscAI CASTG 6 cut(s) 423, 1007, 1303, 1971, 1985, 2709
TseFI GTSAC 2 cut(s) 698, 1788
TseI GCWGC 3 cut(s) 397, 773, 1469
Tsp45I GTSAC 2 cut(s) 698, 1788
TspGWI ACGGA 2 cut(s) 1039, 1137
TspRI CASTG 6 cut(s) 423, 1007, 1303, 1971, 1985, 2709
Van91I CCANNNNNTGG 1 cut(s) 2623
Vha464I CTTAAG 1 cut(s) 2338
VneI GTGCAC 1 cut(s) 1946
VpaK11BI GGWCC 2 cut(s) 2109, 2740
XapI RAATTY 6 cut(s) 137, 475, 956, 1045, 1397, 2326
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 2 cut(s) 2169, 2671
XspI CTAG 8 cut(s) 27, 38, 761, 1103, 1859, 1868, 2007, 2198
ZraI GACGTC 1 cut(s) 853
ZrmI AGTACT 1 cut(s) 25
Zsp2I ATGCAT 2 cut(s) 1771, 2171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.