Rroxscaffold_1G00067000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000159
Physical Location & Seq
Forward (+)
88416235 .. 88419992
3758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067000.1

Sequence Viewer

Length: 2787 bp
ATGGAGTCTTTTGCCTCAAACATAGCACAGATCGTCTTCGAAAAGCTAGCTTCCCGTGCTTACGAAGAAATTCACGTCACATTGAGTGTCAAAGTTGAGCTGAACAAGCTGGAGGAGACTTTATCAAGCATCACTTTACTGCTCCTGGATGCGGAGGAAAAGCAGATGAACAACCGCGTACTCACTGATTGGTTGGGAAAGCTCAAAGATGTTTGCTACGATATTGATGATGAGCTCGATGACTTTGCATTCAAACAGTTGCGGATGCAAGTGCTTATCCGTGGAAGCGTGGCCGGAAAGGTATGCCATCTTTTTTCACGTTGGAATTCACTCGTGTTTCCAATCTATACTGGTCATAAAATCAAACAGATTAGAGCAAGATTGAATGAAATTGCAGCTGCCAAAGATCAGTTTCATCTTAAAGAAAGAGCCGGAGAGTGGCATGGTCTGCACAAGAACAGGGAGACCTATTCATTTGTGCAAGTGTCGGATGTTATTGGGAGAGATAATGATAAAGAAAATATTATCATTCATCTCTTGAAGGACACTGACCATGCAGATCAAAATGTTTCTGTTATTTCTATTACTGGATTAGGAGGTCTGGGAAAGACTACACTTGTTAAATTGGTGTATAATGATTCCACGGTGGTGAGAAACTTTGACTTGAGGATGTGGGTGTGTGTTTCTTATGATTTTGATAATAAGTCATTGGTGCGTAAAATTATTGGTTCTGCAACTAACCAAAAATGTGAAGAGGATAGTTTTGAAAATCTGGCTATTAAGTTGCAAAATGAATTGAAAGACACAAAATTTTTGTTAGTGTTAGATGATGTTTGGGATAAGGAGCCGATGGGGATATCAATTACGAAGTGGATTGAATTGAAAGCTTTACTAAATATTGGTGCTCAGGGTAGTAAAATCATTGTAACAACACGCAATATGTCAGTTGTTTCGCTTTTGGGATCCGTGGATTACCAGCACCCTTTAGAAGGTCTTTCTCACGAGGATTGTATGTCTTTGTTTGTAGAAAGGGCATTTAGGAAAGAAGAAGAAAAGAACTTTCCTCACTTGATGGAAGTTGCAAACAATATTGTGAAGAAGTGTGGAGGGGTTCCTCTGGCTGTGACCACTCTAGGGGGCATGCTCTATTTGAAAAGGGAACTACGTGACTGGTTGCATGTAAGAGATAATGATATATGGAAATTAGAGCAAAGAGAAGACGGTATTCTACCTGCATTGAAACTAAGCTATGATGCACTACCACTCTACTTGAAACCTTGTTTTGCCTTTTGCTCACTTTTCCCTAAGGATTATGAATTTGACAGCATCGAGATCGTCACAATGTGGATGGCACAAGGCTTCCTTCACTCTACCAGAAATGAACAAGATTTTGAAGAGATTGGCCTAGATTACATAAGGCAGCTATGTTCTAGATCTTTCTTTCACATTGAAGAAGATGATTTCTTTTGTATGAGGTTTAAGATGCATGATTTAATTCATGATTTAGCCATCTTGGTGGCACAGGTGGAGTACTCATCTGTCAATTTTCATCAATCTGGTTCTTTTGATAGGGTCCGGCACCTGTCAATACATACAAAGGACTTGTCTGACAATGAGGAAGCCCCTAATTTCATACTCCAGTCAGGCAAGGTGAGAACCATTCTCATCCCAGAAGAGAAGGTTGGCATCACTGGTCGATCTTTCATTAAAAAATGCATTTCCAGATTCAAATATTTGCGGTTGCTAGATCTGCGTAGCTCAACTTTCGACGAGTTGCCAAGTTCCATTGGTAACTTGAGTCACTTGAGATATGTGGACCTATCATACAATTGCCACATAAAAAGGGTTCCTGATTGCATTTGCAAGCTGCAGCATCTACAAACCTTATTGCTTTCACATTGTGAGGAGCTTGAGAAGTTTCCCAAGGACATAGGGAACCTGATTAGCCTCAGGTACTTGGCACTAACTACAAAGAAAACTTGTTTGCCAACAGGAATCGATCGTCTCACTTCTCTTCGCATTTTACATGTTGTTGCATGCCGAAACCTTCTTTCTTTGGAAGGGTTGCCCTGCCTCACTCACCTCAAAATGTTCATTATTGAGGAATGTCCAGTTCTGAGGTCTTTGCCGCATAACATGAAATATTCAACTGCATTAGAGACTTTAATCATCTCTGACTGTGAGAAGCTTGATCTGTTGAGTCTTGAAGAATGCGTTCGAGGTCTAAGATCATTCTGGATCTTGAAATCAAAGTTGAAGAACTTTCCCCTTTGGCTTAAAGAGTGTGCAAGTACTCTACAAAACATATGTATTGCAAACTGTCAGAATCTCATTGCACTTCCGGAATGGCTGCAAAGCTTCAAGTTGCTTGAAAAGCTAGTGATTGAAAACTGCCCCCAAGTGCCAGCTTTGCCAGAGGGGACGCATTGCCTCACAACATTGAGAGAATTGAAGATCAGTGGGTGTCCTCGATTGATGGAAAGATGCAACAGGCACGGAGGTATAGATTGGTTCAAAATTGCTCATGTCACAAAGATAATGCTTGGAGGTGTCAACAACAACACTCCGGTGGATTGTAGCTCAAGTTCCCCTCCCATTCCCCGTCCCCCTCCCCCTCCCCCTCTCCCTCCCCTTCCCCTTCTCCTTCCGCTTGGGACTACTTATGTCCCCTCCCCTTCCCCTTCCTCTAGATCTTCCGCTTGGGACTACTTATTTCCCCTCCCCTTCCCCTTCCCCTTCCCCTTACGACTACTTCTTTTCGGTCGATGCTTCCCATCACGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

928

Amino Acids

106.17

Weight (kDa)

6.86

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 90 1.5e-18 Rx N-terminal domain
NB-ARC PF00931 170 - 350 3e-33 NB-ARC domain
WHD_DRP PF23559 433 - 503 1.7e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 794 3.6e-21 Leucine-rich repeat region
LRR_8 PF13855 579 - 634 4.9e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1240
AccB1I GGYRCC 1 cut(s) 1578
AccII CGCG 1 cut(s) 177
AccIII TCCGGA 1 cut(s) 2341
AciI CCGC 7 cut(s) 152, 175, 262, 1738, 2129, 2648, 2697
AclWI GGATC 3 cut(s) 957, 970, 2246
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 4 cut(s) 69, 325, 809, 1316
AdeI CACNNNGTG 2 cut(s) 1344, 1901
AfaI GTAC 4 cut(s) 180, 1532, 1955, 2293
AfiI CCNNNNNNNGG 4 cut(s) 151, 438, 989, 1134
AflIII ACRYGT 1 cut(s) 2026
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 1665, 1697
AleI CACNNNNGTG 2 cut(s) 647, 2567
Alw21I GWGCWC 2 cut(s) 237, 907
Alw26I GTCTC 4 cut(s) 110, 458, 2009, 2153
AlwI GGATC 3 cut(s) 957, 970, 2246
Aor13HI TCCGGA 1 cut(s) 2341
AoxI GGCC 2 cut(s) 291, 1402
ApeKI GCWGC 6 cut(s) 395, 398, 1420, 1867, 1870, 2350
ApoI RAATTY 4 cut(s) 69, 325, 809, 1316
ArsI GACNNNNNNTTYG 2 cut(s) 1589, 1621
Asp700I GAANNNNTTC 2 cut(s) 69, 2214
AspS9I GGNCC 2 cut(s) 1573, 1816
AsuHPI GGTGA 3 cut(s) 661, 1663, 2072
AsuII TTCGAA 1 cut(s) 39
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 2 cut(s) 1573, 1816
AxyI CCTNAGG 2 cut(s) 1305, 1949
BamHI GGATCC 1 cut(s) 962
BanI GGYRCC 1 cut(s) 1578
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 2 cut(s) 332, 1001
BbsI GAAGAC 2 cut(s) 28, 1224
Bbv12I GWGCWC 2 cut(s) 237, 907
BbvI GCAGC 6 cut(s) 385, 407, 1432, 1854, 1882, 2337
BccI CCATC 7 cut(s) 315, 844, 1066, 1342, 1517, 2470, 2782
BcgI CGANNNNNNTGC 4 cut(s) 227, 261, 1315, 1349
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 4 cut(s) 110, 458, 2009, 2153
BfaI CTAG 7 cut(s) 47, 1133, 1406, 1431, 1745, 2378, 2688
BfmI CTRYAG 1 cut(s) 1868
BfuAI ACCTGC 1 cut(s) 1240
BglII AGATCT 3 cut(s) 1433, 1747, 2690
BisI GCNGC 7 cut(s) 396, 399, 1421, 1868, 1871, 2129, 2351
BlsI GCNGC 7 cut(s) 397, 400, 1422, 1869, 1872, 2130, 2352
BmcAI AGTACT 2 cut(s) 1532, 2293
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 2 cut(s) 1573, 1816
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 1573, 1816
BmiI GGNNCC 7 cut(s) 846, 964, 1113, 1574, 1580, 1848, 1937
BmrFI CCNGG 1 cut(s) 146
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 28, 1224
BpmI CTGGAG 2 cut(s) 131, 1622
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 5 cut(s) 685, 1816, 1825, 1931, 2566
Bsa29I ATCGAT 1 cut(s) 1998
BsaAI YACGTR 1 cut(s) 1166
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 4 cut(s) 280, 642, 966, 1923
BsaWI WCCGGW 2 cut(s) 2341, 2566
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 438, 989, 1134
Bse1I ACTGG 6 cut(s) 355, 592, 1175, 1639, 1696, 2111
Bse21I CCTNAGG 2 cut(s) 1305, 1949
Bse3DI GCAATG 2 cut(s) 2331, 2425
BseAI TCCGGA 1 cut(s) 2341
BseBI CCWGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 1998
BseDI CCNNGG 4 cut(s) 280, 642, 966, 1923
BseGI GGATG 6 cut(s) 154, 270, 496, 675, 1353, 1665
BseLI CCNNNNNNNGG 4 cut(s) 151, 438, 989, 1134
BseMI GCAATG 2 cut(s) 2331, 2425
BseMII CTCAG 3 cut(s) 920, 1963, 2108
BseNI ACTGG 6 cut(s) 355, 592, 1175, 1639, 1696, 2111
BseRI GAGGAG 2 cut(s) 128, 1919
BseXI GCAGC 6 cut(s) 385, 407, 1432, 1854, 1882, 2337
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 2 cut(s) 2002, 2764
BshFI GGCC 2 cut(s) 293, 1404
BshNI GGYRCC 1 cut(s) 1578
BshVI ATCGAT 1 cut(s) 1998
BsiEI CGRYCG 2 cut(s) 2002, 2764
BsiHKAI GWGCWC 2 cut(s) 237, 907
BsiSI CCGG 5 cut(s) 294, 432, 1576, 2342, 2567
BslFI GGGAC 5 cut(s) 2434, 2589, 2651, 2668, 2717
BslI CCNNNNNNNGG 4 cut(s) 151, 438, 989, 1134
BsmAI GTCTC 4 cut(s) 110, 458, 2009, 2153
BsmBI CGTCTC 1 cut(s) 2009
BsmFI GGGAC 5 cut(s) 2434, 2589, 2651, 2668, 2717
BsmI GAATGC 2 cut(s) 248, 2216
BsnI GGCC 2 cut(s) 293, 1404
Bso31I GGTCTC 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 237, 907
Bsp13I TCCGGA 1 cut(s) 2341
BspACI CCGC 7 cut(s) 152, 175, 262, 1738, 2129, 2648, 2697
BspANI GGCC 2 cut(s) 293, 1404
BspCNI CTCAG 3 cut(s) 919, 1962, 2109
BspDI ATCGAT 1 cut(s) 1998
BspEI TCCGGA 1 cut(s) 2341
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 1498
BspLI GGNNCC 7 cut(s) 846, 964, 1113, 1574, 1580, 1848, 1937
BspMAI CTGCAG 1 cut(s) 1872
BspMI ACCTGC 1 cut(s) 1240
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 3 cut(s) 957, 970, 2246
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 1578
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 2331, 2425
BsrI ACTGG 6 cut(s) 355, 592, 1175, 1639, 1696, 2111
BssECI CCNNGG 4 cut(s) 280, 642, 966, 1923
BssSI CACGAG 2 cut(s) 332, 1001
BssT1I CCWWGG 1 cut(s) 1923
Bst2BI CACGAG 2 cut(s) 332, 1001
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 5 cut(s) 258, 646, 1223, 2180, 2321
Bst6I CTCTTC 4 cut(s) 747, 1389, 1668, 2019
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBAI YACGTR 1 cut(s) 1166
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 5 cut(s) 48, 1142, 1865, 2038, 2406
BstDEI CTNAG 6 cut(s) 906, 1244, 1305, 1949, 2117, 2225
BstDSI CCRYGG 3 cut(s) 280, 642, 966
BstENI CCTNNNNNAGG 1 cut(s) 987
BstF5I GGATG 6 cut(s) 154, 270, 496, 675, 1353, 1665
BstFNI CGCG 1 cut(s) 177
BstMAI GTCTC 4 cut(s) 110, 458, 2009, 2153
BstMCI CGRYCG 2 cut(s) 2002, 2764
BstMWI GCNNNNNNNGC 6 cut(s) 56, 106, 448, 1750, 2374, 2410
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 4 cut(s) 1144, 1181, 2030, 2040
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 1868
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 6 cut(s) 385, 407, 1432, 1854, 1882, 2337
BstV2I GAAGAC 2 cut(s) 28, 1224
BstX2I RGATCY 5 cut(s) 962, 1433, 1747, 2238, 2690
BstXI CCANNNNNNTGG 1 cut(s) 1516
BstYI RGATCY 5 cut(s) 962, 1433, 1747, 2238, 2690
Bsu15I ATCGAT 1 cut(s) 1998
Bsu36I CCTNAGG 2 cut(s) 1305, 1949
BsuRI GGCC 2 cut(s) 293, 1404
BsuTUI ATCGAT 1 cut(s) 1998
BtgI CCRYGG 3 cut(s) 280, 642, 966
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 6 cut(s) 154, 270, 496, 675, 1353, 1665
BtsIMutI CAGTG 4 cut(s) 183, 546, 1689, 2464
BveI ACCTGC 1 cut(s) 1240
Cac8I GCNNGC 5 cut(s) 48, 1142, 1865, 2038, 2406
CciI TCATGA 1 cut(s) 1498
Cfr13I GGNCC 2 cut(s) 1573, 1816
ClaI ATCGAT 1 cut(s) 1998
CseI GACGC 1 cut(s) 2431
Csp6I GTAC 4 cut(s) 179, 1531, 1954, 2292
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviQI GTAC 4 cut(s) 179, 1531, 1954, 2292
DdeI CTNAG 6 cut(s) 906, 1244, 1305, 1949, 2117, 2225
DraIII CACNNNGTG 2 cut(s) 1344, 1901
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 4 cut(s) 747, 1389, 1668, 2019
EarI CTCTTC 4 cut(s) 747, 1389, 1668, 2019
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 1923
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 858
Eco47I GGWCC 2 cut(s) 1573, 1816
Eco53kI GAGCTC 1 cut(s) 235
Eco81I CCTNAGG 2 cut(s) 1305, 1949
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 987
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 144
EcoRV GATATC 1 cut(s) 858
EcoT14I CCWWGG 1 cut(s) 1923
EcoT22I ATGCAT 2 cut(s) 1488, 1718
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 1923
Esp3I CGTCTC 1 cut(s) 2009
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 1347, 1379
FaqI GGGAC 5 cut(s) 2434, 2589, 2651, 2668, 2717
FauNDI CATATG 1 cut(s) 2306
Fnu4HI GCNGC 7 cut(s) 396, 399, 1421, 1868, 1871, 2129, 2351
FokI GGATG 6 cut(s) 161, 277, 503, 682, 1360, 1652
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 7 cut(s) 396, 399, 1421, 1868, 1871, 2129, 2351
FspBI CTAG 7 cut(s) 47, 1133, 1406, 1431, 1745, 2378, 2688
GluI GCNGC 7 cut(s) 396, 399, 1421, 1868, 1871, 2129, 2351
GsuI CTGGAG 2 cut(s) 131, 1622
HaeIII GGCC 2 cut(s) 293, 1404
HapII CCGG 5 cut(s) 294, 432, 1576, 2342, 2567
HgaI GACGC 1 cut(s) 2431
HincII GTYRAC 1 cut(s) 2554
HindII GTYRAC 1 cut(s) 2554
HindIII AAGCTT 3 cut(s) 885, 2186, 2356
HinfI GANTC 7 cut(s) 5, 638, 1725, 1798, 1995, 2200, 2326
HpaII CCGG 5 cut(s) 294, 432, 1576, 2342, 2567
HphI GGTGA 3 cut(s) 661, 1663, 2072
Hpy166II GTNNAC 2 cut(s) 1816, 2554
Hpy188I TCNGA 5 cut(s) 490, 1609, 2118, 2176, 2325
Hpy8I GTNNAC 2 cut(s) 1816, 2554
Hpy99I CGWCG 1 cut(s) 1772
HpyCH4III ACNGT 5 cut(s) 258, 646, 1223, 2180, 2321
HpyCH4IV ACGT 3 cut(s) 75, 319, 1165
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 106, 448, 1750, 2374, 2410
HpyF3I CTNAG 6 cut(s) 906, 1244, 1305, 1949, 2117, 2225
HpySE526I ACGT 3 cut(s) 75, 319, 1165
Kpn2I TCCGGA 1 cut(s) 2341
LmnI GCTCC 3 cut(s) 147, 844, 1906
Lsp1109I GCAGC 6 cut(s) 385, 407, 1432, 1854, 1882, 2337
MaeI CTAG 7 cut(s) 47, 1133, 1406, 1431, 1745, 2378, 2688
MaeII ACGT 3 cut(s) 75, 319, 1165
MaeIII GTNAC 8 cut(s) 76, 925, 1123, 1166, 1336, 1790, 1799, 2527
MfeI CAATTG 1 cut(s) 1828
MflI RGATCY 5 cut(s) 962, 1433, 1747, 2238, 2690
MhlI GDGCHC 2 cut(s) 237, 907
MlyI GAGTC 3 cut(s) 14, 1807, 2209
MmeI TCCRAC 2 cut(s) 302, 468
Mph1103I ATGCAT 2 cut(s) 1488, 1718
MroI TCCGGA 1 cut(s) 2341
MroXI GAANNNNTTC 2 cut(s) 69, 2214
MseI TTAA 8 cut(s) 420, 621, 780, 1479, 1493, 1707, 2165, 2277
MslI CAYNNNNRTG 3 cut(s) 647, 1514, 2567
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 5 cut(s) 294, 432, 1576, 2342, 2567
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 1828
Mva1269I GAATGC 2 cut(s) 248, 2216
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 6 cut(s) 56, 106, 448, 1750, 2374, 2410
NdeI CATATG 1 cut(s) 2306
NheI GCTAGC 1 cut(s) 46
NlaIV GGNNCC 7 cut(s) 846, 964, 1113, 1574, 1580, 1848, 1937
NmuCI GTSAC 6 cut(s) 76, 1123, 1166, 1336, 1799, 2527
NsiI ATGCAT 2 cut(s) 1488, 1718
NspI RCATGY 4 cut(s) 1144, 1181, 2030, 2040
NspV TTCGAA 1 cut(s) 39
OliI CACNNNNGTG 2 cut(s) 647, 2567
PaeI GCATGC 2 cut(s) 1144, 2040
PagI TCATGA 1 cut(s) 1498
PciI ACATGT 1 cut(s) 2026
PctI GAATGC 2 cut(s) 248, 2216
PdmI GAANNNNTTC 2 cut(s) 69, 2214
PfeI GAWTC 4 cut(s) 638, 1725, 1995, 2326
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 7 cut(s) 397, 400, 1422, 1869, 1872, 2130, 2352
Ple19I CGATCG 1 cut(s) 2002
PleI GAGTC 3 cut(s) 13, 1806, 2208
PpsI GAGTC 3 cut(s) 13, 1806, 2208
Ppu21I YACGTR 1 cut(s) 1166
PscI ACATGT 1 cut(s) 2026
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 7 cut(s) 846, 964, 1113, 1574, 1580, 1848, 1937
PspPI GGNCC 2 cut(s) 1573, 1816
PsrI GAACNNNNNNTAC 2 cut(s) 2569, 2601
PstI CTGCAG 1 cut(s) 1872
PsuI RGATCY 5 cut(s) 962, 1433, 1747, 2238, 2690
PvuI CGATCG 1 cut(s) 2002
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 4 cut(s) 180, 1532, 1955, 2293
RsaNI GTAC 4 cut(s) 179, 1531, 1954, 2292
RseI CAYNNNNRTG 3 cut(s) 647, 1514, 2567
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 8 cut(s) 420, 621, 780, 1479, 1493, 1707, 2165, 2277
SatI GCNGC 7 cut(s) 396, 399, 1421, 1868, 1871, 2129, 2351
Sau96I GGNCC 2 cut(s) 1573, 1816
ScaI AGTACT 2 cut(s) 1532, 2293
SchI GAGTC 3 cut(s) 14, 1807, 2209
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 237, 907
SfcI CTRYAG 1 cut(s) 1868
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 2 cut(s) 1573, 1816
SmiMI CAYNNNNRTG 3 cut(s) 647, 1514, 2567
SmlI CTYRAG 5 cut(s) 664, 1795, 1804, 1910, 2581
SmoI CTYRAG 5 cut(s) 664, 1795, 1804, 1910, 2581
SphI GCATGC 2 cut(s) 1144, 2040
SsiI CCGC 7 cut(s) 152, 175, 262, 1738, 2129, 2648, 2697
SspI AATATT 5 cut(s) 523, 898, 1090, 1733, 2144
SspMI CTAG 7 cut(s) 47, 1133, 1406, 1431, 1745, 2378, 2688
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 1923
TaaI ACNGT 5 cut(s) 258, 646, 1223, 2180, 2321
TaiI ACGT 3 cut(s) 78, 322, 1168
TaqI TCGA 9 cut(s) 39, 237, 1329, 1696, 1767, 1998, 2218, 2470, 2764
TaqII GACCGA 1 cut(s) 2750
TatI WGTACW 2 cut(s) 1530, 2291
TauI GCSGC 1 cut(s) 2131
TfiI GAWTC 4 cut(s) 638, 1725, 1995, 2326
Tru1I TTAA 8 cut(s) 420, 621, 780, 1479, 1493, 1707, 2165, 2277
Tru9I TTAA 8 cut(s) 420, 621, 780, 1479, 1493, 1707, 2165, 2277
TscAI CASTG 4 cut(s) 190, 553, 1696, 2464
TseFI GTSAC 6 cut(s) 76, 1123, 1166, 1336, 1799, 2527
TseI GCWGC 6 cut(s) 395, 398, 1420, 1867, 1870, 2350
Tsp45I GTSAC 6 cut(s) 76, 1123, 1166, 1336, 1799, 2527
TspGWI ACGGA 3 cut(s) 269, 955, 2511
TspRI CASTG 4 cut(s) 190, 553, 1696, 2464
VpaK11BI GGWCC 2 cut(s) 1573, 1816
XagI CCTNNNNNAGG 1 cut(s) 987
XapI RAATTY 4 cut(s) 69, 325, 809, 1316
XbaI TCTAGA 2 cut(s) 1430, 2687
XceI RCATGY 4 cut(s) 1144, 1181, 2030, 2040
XmnI GAANNNNTTC 2 cut(s) 69, 2214
XspI CTAG 7 cut(s) 47, 1133, 1406, 1431, 1745, 2378, 2688
ZrmI AGTACT 2 cut(s) 1532, 2293
Zsp2I ATGCAT 2 cut(s) 1488, 1718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.