RLG00000018355

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000159
Physical Location & Seq
Reverse (-)
28682438 .. 28684223
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018355

Sequence Viewer

Length: 1566 bp
ATGGAGTTTGCCACCTGCATTGCAGAGAACATCTTGGGTAGGCTAGCTTTCTATGCTTCCCAAGAGGTCTACTTCACATGGGGTGCCCAACTTGAGCTCACCAAGATCAACAAGACCTTATCTACCATCAAACTAGTGCTTCAGGATGCAGAGAAGAAGCAAGTGAAGAATTCCCTAATCACTCGTTGGTTGGGAGATCTCAAAGATGTTTGCCATGACATTGACGATGTCCTTGAGGAACTAGAGTTCCAAAAGCTGCAGAAGAAAGTTTTGGTCAACAACAGTGGGAGAGTCAAAGGACAGAGAGAGTTTTCTCTTGTTGAAGTTGCTGAGCTAGCCGAAGATCATCGTGCGCCCCACCAAGTGCACAATGACAGGAGGATGACTACCTCTAAAGTTAAGGCTTCAAATGTTATTGGAAGAGATGACGATAAAAAACAGATTAGCAAGCATCTCTTGAATGACACTAATTTCTCCAGTGAGGAGAATGTTTATGTTGTTTCCATAATTGGGTTAGGAGGGGTGGGGAAAACAACACTTGCTAAATTGGTGTACAATGATAGCATGGTAGAAGAAAATTTTGAGACAAAGATGTGGATATGCGTCTCAGACAACTTTGATATCCAAACGTTAATTCGAGGTATTACTAATGCTGCAAATGGACCAAAATGTGAGGATGAAAGTTTGGATCTTATGGGAAGAAAGTTGCAAGATACTCTTAGAGGTAAAAAATTTTCATTGGTGTTGGACGATATTTGGGATACAGAGTCGATTGGAGTAACAGTTGAAAAATGTATTGATTTAAAAACCTTGTTAAATGTGGGAGCTAATGGAAGCAAAATCATTATAACAACACGAATTAAATCAGTTGCTTTACTTAAATTGGAGAAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAGTTGCTGCTGTTGGGAGCATGCTTTATTTGAATACCGATGAACACCAATGGTCTAGTGTCAGAAATGATGACATGTGGAGTATAGGGAATGACAATATTTTATCTGCACTCAAATTGAGTTCTGATGCATTGCCACAACACTTGAAATCGTGTTTTGCATTTTGTTCACTTTTCCCAAAAGATTATGTGTTCAAGAGTGCATTGTTGGTTCCACTGTGGACAGCACAAGGATACCTCAAGACATCTAAGAGAAATGAAGATTTTGAGCTGATGGGTTTAGACTATATAAAGGAGTTTTGCTCTAGATCGTTGTTTCAAGTCGAGACAGATTTCAAAACATCCATAACTTTTAAAATACGTGATCTCATTCATGCTTTAGCAATTTCAGTGGCACAAGTAGAGTACTCCACAATTAATTTTCGTCCCTCTGGTGATTTTGAAATGGTTCGACATGTGTCAATATCTAAAAAAGACTTGCTTGGAGAAGAGGCACAAGTTCCTGAATTCATGCTCAAGTCAAAGAAATTGCGAACCATTCTAATTCCTAAAGATTGCCAGATGAATCAAGGTTTTGTGAAGACATGCATCTCGAAATTCAAATGTATGCGGGTGCTCGATGTCAATGGGTCCCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

522

Amino Acids

58.74

Weight (kDa)

7.09

Isoelectric Point (pI)

25.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 94 1.9e-17 Rx N-terminal domain
NB-ARC PF00931 143 - 291 9.1e-25 NB-ARC domain
WHD_DRP PF23559 366 - 436 7e-17 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
AarI CACCTGC 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 1534
AclI AACGTT 1 cut(s) 629
AclWI GGATC 1 cut(s) 696
AcsI RAATTY 5 cut(s) 169, 577, 731, 1430, 1520
AcuI CTGAAG 1 cut(s) 125
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 2 cut(s) 554, 1331
AfiI CCNNNNNNNGG 1 cut(s) 510
AflIII ACRYGT 2 cut(s) 999, 1378
AhlI ACTAGT 1 cut(s) 133
AjuI GAANNNNNNNTTGG 4 cut(s) 254, 286, 618, 650
AloI GAACNNNNNNTCC 4 cut(s) 230, 231, 262, 263
AluBI AGCT 6 cut(s) 47, 97, 256, 334, 827, 1195
AluI AGCT 6 cut(s) 47, 97, 256, 334, 827, 1195
Alw21I GWGCWC 3 cut(s) 99, 369, 1542
Alw26I GTCTC 3 cut(s) 578, 610, 1244
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 1 cut(s) 696
AlwNI CAGNNNCTG 1 cut(s) 932
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 3 cut(s) 256, 653, 932
ApoI RAATTY 5 cut(s) 169, 577, 731, 1430, 1520
AseI ATTAAT 1 cut(s) 1341
Asp700I GAANNNNTTC 1 cut(s) 1371
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 662, 1554
AsuHPI GGTGA 2 cut(s) 91, 1370
AsuNHI GCTAGC 2 cut(s) 43, 334
AvaII GGWCC 2 cut(s) 662, 1554
BaeGI GKGCMC 2 cut(s) 88, 369
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 1511
Bbv12I GWGCWC 3 cut(s) 99, 369, 1542
BbvI GCAGC 3 cut(s) 243, 640, 919
BccI CCATC 2 cut(s) 134, 1192
BciVI GTATCC 2 cut(s) 754, 1151
BcoDI GTCTC 3 cut(s) 578, 610, 1244
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 6 cut(s) 44, 134, 242, 335, 981, 1230
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 2 cut(s) 754, 1151
BglII AGATCT 1 cut(s) 196
BisI GCNGC 3 cut(s) 257, 654, 933
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 3 cut(s) 258, 655, 934
BmcAI AGTACT 1 cut(s) 1331
Bme18I GGWCC 2 cut(s) 662, 1554
BmgT120I GGNCC 2 cut(s) 662, 1554
BmiI GGNNCC 4 cut(s) 85, 1137, 1555, 1556
BmsI GCATC 4 cut(s) 136, 460, 1042, 1521
BmtI GCTAGC 2 cut(s) 47, 338
BoxI GACNNNNGTC 1 cut(s) 1381
BpiI GAAGAC 1 cut(s) 1511
BplI GAGNNNNNCTC 2 cut(s) 1211, 1243
BpmI CTGGAG 1 cut(s) 460
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 4 cut(s) 113, 254, 1148, 1424
BsaAI YACGTR 1 cut(s) 1286
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 477
Bse3DI GCAATG 2 cut(s) 18, 1055
BseGI GGATG 4 cut(s) 151, 387, 682, 1265
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMI GCAATG 2 cut(s) 18, 1055
BseMII CTCAG 2 cut(s) 321, 621
BseNI ACTGG 1 cut(s) 477
BseRI GAGGAG 2 cut(s) 497, 927
BseSI GKGCMC 2 cut(s) 88, 369
BseXI GCAGC 3 cut(s) 243, 640, 919
BsgI GTGCAG 1 cut(s) 1017
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 3 cut(s) 99, 369, 1542
BslFI GGGAC 2 cut(s) 1335, 1540
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 3 cut(s) 578, 610, 1244
BsmBI CGTCTC 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 1335, 1540
Bsp1286I GDGCHC 4 cut(s) 88, 99, 369, 1542
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 1 cut(s) 1534
BspCNI CTCAG 2 cut(s) 322, 620
BspLI GGNNCC 4 cut(s) 85, 1137, 1555, 1556
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 23
BspOI GCTAGC 2 cut(s) 47, 338
BspPI GGATC 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 18, 1055
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 1 cut(s) 477
BssMI GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
Bst4CI ACNGT 3 cut(s) 284, 784, 1143
Bst6I CTCTTC 2 cut(s) 415, 1407
BstAPI GCANNNNNTGC 1 cut(s) 932
BstAUI TGTACA 1 cut(s) 552
BstBAI YACGTR 1 cut(s) 1286
BstC8I GCNNGC 4 cut(s) 45, 336, 449, 947
BstDEI CTNAG 4 cut(s) 330, 607, 719, 1173
BstF5I GGATG 4 cut(s) 151, 387, 682, 1265
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 6 cut(s) 108, 199, 346, 691, 1235, 1291
BstMAI GTCTC 3 cut(s) 578, 610, 1244
BstMBI GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
BstMWI GCNNNNNNNGC 3 cut(s) 53, 335, 932
BstNSI RCATGY 4 cut(s) 949, 1003, 1382, 1512
BstPAI GACNNNNGTC 1 cut(s) 1381
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 2 cut(s) 88, 369
BstV1I GCAGC 3 cut(s) 243, 640, 919
BstV2I GAAGAC 1 cut(s) 1511
BstX2I RGATCY 2 cut(s) 196, 688
BstYI RGATCY 2 cut(s) 196, 688
BsuI GTATCC 2 cut(s) 754, 1151
BtsCI GGATG 4 cut(s) 151, 387, 682, 1265
BtsIMutI CAGTG 4 cut(s) 289, 484, 1139, 1320
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 4 cut(s) 45, 336, 449, 947
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 1 cut(s) 355
Cfr13I GGNCC 2 cut(s) 662, 1554
CseI GACGC 1 cut(s) 592
Csp6I GTAC 2 cut(s) 553, 1330
CspCI CAANNNNNGTGG 7 cut(s) 36, 265, 300, 1050, 1085, 1296, 1331
CviAII CATG 9 cut(s) 78, 215, 565, 946, 1000, 1298, 1379, 1435, 1509
CviJI RGCY 9 cut(s) 43, 47, 97, 256, 334, 338, 404, 827, 1195
CviKI_1 RGCY 9 cut(s) 43, 47, 97, 256, 334, 338, 404, 827, 1195
CviQI GTAC 2 cut(s) 553, 1330
DdeI CTNAG 4 cut(s) 330, 607, 719, 1173
DpnI GATC 6 cut(s) 107, 198, 345, 690, 1234, 1290
DpnII GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
DraI TTTAAA 2 cut(s) 804, 1279
DraIII CACNNNGTG 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 415, 1407
EarI CTCTTC 2 cut(s) 415, 1407
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 662, 1554
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 1554
EcoRI GAATTC 2 cut(s) 169, 1430
EcoRV GATATC 1 cut(s) 622
EcoT22I ATGCAT 2 cut(s) 1057, 1514
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 610
FaeI CATG 9 cut(s) 81, 218, 568, 949, 1003, 1301, 1382, 1438, 1512
FalI AAGNNNNNCTT 6 cut(s) 440, 472, 702, 734, 1389, 1421
FaqI GGGAC 2 cut(s) 1335, 1540
FatI CATG 9 cut(s) 77, 214, 564, 945, 999, 1297, 1378, 1434, 1508
FauI CCCGC 1 cut(s) 1527
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 257, 654, 933
FokI GGATG 4 cut(s) 158, 394, 689, 1252
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 257, 654, 933
FspBI CTAG 6 cut(s) 44, 134, 242, 335, 981, 1230
GlaI GCGC 1 cut(s) 354
GluI GCNGC 3 cut(s) 257, 654, 933
GsuI CTGGAG 1 cut(s) 460
HgaI GACGC 1 cut(s) 592
HhaI GCGC 1 cut(s) 355
Hin1II CATG 9 cut(s) 81, 218, 568, 949, 1003, 1301, 1382, 1438, 1512
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 291, 767, 1489
HphI GGTGA 2 cut(s) 91, 1370
Hpy166II GTNNAC 6 cut(s) 70, 277, 367, 553, 1094, 1146
Hpy188I TCNGA 3 cut(s) 610, 989, 1051
Hpy188III TCNNGA 8 cut(s) 143, 457, 1120, 1165, 1230, 1249, 1427, 1516
Hpy8I GTNNAC 6 cut(s) 70, 277, 367, 553, 1094, 1146
HpyAV CCTTC 1 cut(s) 883
HpyCH4III ACNGT 3 cut(s) 284, 784, 1143
HpyCH4IV ACGT 2 cut(s) 629, 1285
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 335, 932
HpyF3I CTNAG 4 cut(s) 330, 607, 719, 1173
HpySE526I ACGT 2 cut(s) 629, 1285
Hsp92II CATG 9 cut(s) 81, 218, 568, 949, 1003, 1301, 1382, 1438, 1512
HspAI GCGC 1 cut(s) 353
KflI GGGWCCC 1 cut(s) 1554
Kzo9I GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
LmnI GCTCC 2 cut(s) 824, 942
LpnPI CCDG 7 cut(s) 28, 128, 361, 490, 1341, 1440, 1496
Lsp1109I GCAGC 3 cut(s) 243, 640, 919
LweI GCATC 4 cut(s) 136, 460, 1042, 1521
MaeI CTAG 6 cut(s) 44, 134, 242, 335, 981, 1230
MaeII ACGT 2 cut(s) 629, 1285
MaeIII GTNAC 1 cut(s) 778
MalI GATC 6 cut(s) 107, 198, 345, 690, 1234, 1290
MboI GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
MflI RGATCY 2 cut(s) 196, 688
MhlI GDGCHC 4 cut(s) 88, 99, 369, 1542
MlyI GAGTC 2 cut(s) 300, 776
MmeI TCCRAC 1 cut(s) 726
Mph1103I ATGCAT 2 cut(s) 1057, 1514
MroXI GAANNNNTTC 1 cut(s) 1371
MseI TTAA 8 cut(s) 399, 632, 803, 815, 861, 879, 1278, 1341
MwoI GCNNNNNNNGC 3 cut(s) 53, 335, 932
NdeII GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
NheI GCTAGC 2 cut(s) 43, 334
NlaIII CATG 9 cut(s) 81, 218, 568, 949, 1003, 1301, 1382, 1438, 1512
NlaIV GGNNCC 4 cut(s) 85, 1137, 1555, 1556
NsiI ATGCAT 2 cut(s) 1057, 1514
NspI RCATGY 4 cut(s) 949, 1003, 1382, 1512
PaeI GCATGC 1 cut(s) 949
PaqCI CACCTGC 1 cut(s) 23
PciI ACATGT 2 cut(s) 999, 1378
PdmI GAANNNNTTC 1 cut(s) 1371
PfeI GAWTC 1 cut(s) 1489
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 258, 655, 934
PleI GAGTC 2 cut(s) 299, 775
PpsI GAGTC 2 cut(s) 299, 775
Ppu21I YACGTR 1 cut(s) 1286
PpuMI RGGWCCY 1 cut(s) 1554
PscI ACATGT 2 cut(s) 999, 1378
PshAI GACNNNNGTC 1 cut(s) 1381
PshBI ATTAAT 1 cut(s) 1341
PsiI TTATAA 1 cut(s) 848
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 629
Psp5II RGGWCCY 1 cut(s) 1554
PspN4I GGNNCC 4 cut(s) 85, 1137, 1555, 1556
PspPI GGNCC 2 cut(s) 662, 1554
PspPPI RGGWCCY 1 cut(s) 1554
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 2 cut(s) 196, 688
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 2 cut(s) 554, 1331
RsaNI GTAC 2 cut(s) 553, 1330
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 8 cut(s) 399, 632, 803, 815, 861, 879, 1278, 1341
SatI GCNGC 3 cut(s) 257, 654, 933
Sau3AI GATC 6 cut(s) 105, 196, 343, 688, 1232, 1288
Sau96I GGNCC 2 cut(s) 662, 1554
ScaI AGTACT 1 cut(s) 1331
SchI GAGTC 2 cut(s) 300, 776
SduI GDGCHC 4 cut(s) 88, 99, 369, 1542
SfaNI GCATC 4 cut(s) 136, 460, 1042, 1521
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 662, 1554
SmlI CTYRAG 4 cut(s) 92, 233, 1163, 1439
SmoI CTYRAG 4 cut(s) 92, 233, 1163, 1439
SpeI ACTAGT 1 cut(s) 133
SphI GCATGC 1 cut(s) 949
SsiI CCGC 1 cut(s) 1534
SspI AATATT 1 cut(s) 1024
SspMI CTAG 6 cut(s) 44, 134, 242, 335, 981, 1230
SstI GAGCTC 1 cut(s) 99
TaaI ACNGT 3 cut(s) 284, 784, 1143
TaiI ACGT 2 cut(s) 632, 1288
TaqI TCGA 6 cut(s) 637, 770, 1248, 1375, 1517, 1542
TatI WGTACW 2 cut(s) 552, 1329
TfiI GAWTC 1 cut(s) 1489
Tru1I TTAA 8 cut(s) 399, 632, 803, 815, 861, 879, 1278, 1341
Tru9I TTAA 8 cut(s) 399, 632, 803, 815, 861, 879, 1278, 1341
TscAI CASTG 4 cut(s) 289, 484, 1146, 1320
TseI GCWGC 3 cut(s) 256, 653, 932
TspDTI ATGAA 7 cut(s) 693, 726, 981, 1197, 1286, 1423, 1502
TspRI CASTG 4 cut(s) 289, 484, 1146, 1320
Tth111I GACNNNGTC 1 cut(s) 227
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 662, 1554
VspI ATTAAT 1 cut(s) 1341
XapI RAATTY 5 cut(s) 169, 577, 731, 1430, 1520
XbaI TCTAGA 1 cut(s) 1229
XceI RCATGY 4 cut(s) 949, 1003, 1382, 1512
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 1371
XspI CTAG 6 cut(s) 44, 134, 242, 335, 981, 1230
ZrmI AGTACT 1 cut(s) 1331
Zsp2I ATGCAT 2 cut(s) 1057, 1514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.