Rh2AG263900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000159
Physical Location & Seq
Forward (+)
30628023 .. 30631087
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG263900.1

Sequence Viewer

Length: 675 bp
ATGCTGAGATCAGGGGAAGGTCCTCAAAGTCTTCGATCATTGGTTATCTGGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTCACTGAAGATTCTGCAGATACTCTACAGAGCATAGCACTTGATCGATGTCATAATCTCACGGCACTTCCGGAGTTGATGAAATTCAAATTCCTCGAGCAACTACGCATTGAAGAATGCTCCAAATTGTCGGCGTTGCCGCAAGGGTTGCATTGCCTTACTGAGTTGAGAGAACTGTACATTGAAGACTGTCCTGAATTGAGCAAAAGCTGCGTAAGGCAATTAAAAGGTGAGGAGTTGTCAAAGATGGGACGTCACGTGACGATTACACTAGACGCTGATGATGAAGAAGATGAAGCCGACAATCGCTTGGCCGTCGGTAGGATCAGTATCCCTGCTGGGTTTGGAATCAGATCAGAGGAAGAAGAGGATCACTACCTACAACTCTACAAGGTGTACACAGTGGAGGGGAAGCTGAAAGGGACTCTCAGGAAGAGCTTCAGGTGGCTCAAGGAGACCTGTGATAGAGTGGTCTATGGCGGTCGTGTATATGCTAGCTACTACTTTTATGATCTTTACCTTGTCCAATCTTTCACAACTGCATTGCAAGATAAAGTACCAATAGCTGCTTATTCAATAAAGTATGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

224

Amino Acids

25.67

Weight (kDa)

5.27

Isoelectric Point (pI)

71.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 8 - 67 2.8e-09 R13L1/DRL21 LRRs
DUF3511 PF12023 157 - 187 3.5e-14 Domain of unknown function (DUF3511)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 337
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 2 cut(s) 221, 561
AclWI GGATC 2 cut(s) 413, 459
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 3 cut(s) 52, 164, 170
AcuI CTGAAG 2 cut(s) 108, 505
AcvI CACGTG 1 cut(s) 340
AcyI GRCGYC 1 cut(s) 334
AfaI GTAC 3 cut(s) 260, 479, 639
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 5 cut(s) 57, 169, 194, 266, 657
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 5 cut(s) 291, 496, 519, 579, 647
AluI AGCT 5 cut(s) 291, 496, 519, 579, 647
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 2 cut(s) 413, 459
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 291, 647
ApoI RAATTY 3 cut(s) 52, 164, 170
Asp700I GAANNNNTTC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 323
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 20
BbrPI CACGTG 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 23, 273
BbvI GCAGC 2 cut(s) 278, 634
BccI CCATC 1 cut(s) 322
BceAI ACGGC 2 cut(s) 159, 380
BciVI GTATCC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 2 cut(s) 353, 576
BfmI CTRYAG 2 cut(s) 96, 107
BfuI GTATCC 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 79
BisI GCNGC 3 cut(s) 221, 292, 648
BlsI GCNGC 3 cut(s) 222, 293, 649
Bme18I GGWCC 1 cut(s) 20
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 20
BmtI GCTAGC 1 cut(s) 579
BpiI GAAGAC 2 cut(s) 23, 273
BpuEI CTTGAG 1 cut(s) 515
Bsa29I ATCGAT 1 cut(s) 127
BsaAI YACGTR 1 cut(s) 340
BsaBI GATNNNNATC 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 334
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse3DI GCAATG 2 cut(s) 232, 623
Bse8I GATNNNNATC 1 cut(s) 410
BseAI TCCGGA 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 1 cut(s) 77
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 2 cut(s) 232, 623
BseMII CTCAG 2 cut(s) 234, 523
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 278, 634
BseYI CCCAGC 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 565
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 345, 517
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 1 cut(s) 530
BsmFI GGGAC 2 cut(s) 345, 517
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 395
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 151
Bsp1407I TGTACA 2 cut(s) 258, 477
Bsp143I GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
BspACI CCGC 2 cut(s) 221, 561
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 235, 522
BspDI ATCGAT 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 100
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 2 cut(s) 413, 459
BspQI GCTCTTC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 530
BsrDI GCAATG 2 cut(s) 232, 623
BsrGI TGTACA 2 cut(s) 258, 477
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
BssNI GRCGYC 1 cut(s) 334
BssT1I CCWWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 258, 272, 484
Bst6I CTCTTC 2 cut(s) 441, 509
BstACI GRCGYC 1 cut(s) 334
BstAPI GCANNNNNTGC 2 cut(s) 229, 291
BstAUI TGTACA 2 cut(s) 258, 477
BstBAI YACGTR 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 3 cut(s) 5, 243, 509
BstKTI GATC 7 cut(s) 11, 38, 127, 408, 437, 454, 595
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 3 cut(s) 79, 229, 291
BstSFI CTRYAG 2 cut(s) 96, 107
BstV1I GCAGC 2 cut(s) 278, 634
BstV2I GAAGAC 2 cut(s) 23, 273
Bsu15I ATCGAT 1 cut(s) 127
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 84, 489
Cac8I GCNNGC 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 127
CseI GACGC 1 cut(s) 365
Csp6I GTAC 3 cut(s) 259, 478, 638
CviQI GTAC 3 cut(s) 259, 478, 638
DdeI CTNAG 3 cut(s) 5, 243, 509
DpnI GATC 7 cut(s) 10, 37, 126, 407, 436, 453, 594
DpnII GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 441, 509
EarI CTCTTC 2 cut(s) 441, 509
Eco130I CCWWGG 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 2 cut(s) 108, 505
Eco72I CACGTG 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaiI YATR 7 cut(s) 116, 135, 558, 571, 573, 591, 666
FaqI GGGAC 2 cut(s) 345, 517
Fnu4HI GCNGC 3 cut(s) 221, 292, 648
Fsp4HI GCNGC 3 cut(s) 221, 292, 648
FspBI CTAG 2 cut(s) 353, 576
GluI GCNGC 3 cut(s) 221, 292, 648
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 365
Hin1I GRCGYC 1 cut(s) 334
HinfI GANTC 3 cut(s) 92, 429, 505
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 478, 480
Hpy188I TCNGA 2 cut(s) 434, 439
Hpy188III TCNNGA 4 cut(s) 49, 152, 275, 511
Hpy8I GTNNAC 2 cut(s) 478, 480
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 1 cut(s) 11
HpyCH4III ACNGT 3 cut(s) 258, 272, 484
HpyCH4IV ACGT 2 cut(s) 334, 339
HpyCH4V TGCA 4 cut(s) 98, 232, 623, 628
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 229, 291
HpyF3I CTNAG 3 cut(s) 5, 243, 509
HpySE526I ACGT 2 cut(s) 334, 339
Hsp92I GRCGYC 1 cut(s) 334
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 8 cut(s) 34, 165, 288, 405, 429, 496, 508, 553
Lsp1109I GCAGC 2 cut(s) 278, 634
MaeI CTAG 2 cut(s) 353, 576
MaeII ACGT 2 cut(s) 334, 339
MaeIII GTNAC 2 cut(s) 335, 340
MalI GATC 7 cut(s) 10, 37, 126, 407, 436, 453, 594
MboI GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
MboII GAAGA 9 cut(s) 23, 101, 206, 278, 380, 383, 455, 458, 526
MluCI AATT 6 cut(s) 52, 164, 170, 206, 278, 302
MlyI GAGTC 1 cut(s) 499
MnlI CCTC 7 cut(s) 33, 58, 185, 307, 433, 442, 481
MroI TCCGGA 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 1 cut(s) 305
MspI CCGG 1 cut(s) 152
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 79, 229, 291
NdeII GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
NheI GCTAGC 1 cut(s) 575
NmuCI GTSAC 2 cut(s) 335, 340
PaeR7I CTCGAG 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 509
PctI GAATGC 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 92, 429
PkrI GCNGC 3 cut(s) 222, 293, 649
PleI GAGTC 1 cut(s) 499
PmaCI CACGTG 1 cut(s) 340
PmlI CACGTG 1 cut(s) 340
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 1 cut(s) 340
PpuMI RGGWCCY 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 20
PspCI CACGTG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 20
PspPPI RGGWCCY 1 cut(s) 20
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 1 cut(s) 100
RsaI GTAC 3 cut(s) 260, 479, 639
RsaNI GTAC 3 cut(s) 259, 478, 638
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 3 cut(s) 221, 292, 648
Sau3AI GATC 7 cut(s) 8, 35, 124, 405, 434, 451, 592
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 499
SfcI CTRYAG 2 cut(s) 96, 107
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 176, 530
SmoI CTYRAG 2 cut(s) 176, 530
Sse9I AATT 6 cut(s) 52, 164, 170, 206, 278, 302
SsiI CCGC 2 cut(s) 221, 561
SspMI CTAG 2 cut(s) 353, 576
StyI CCWWGG 1 cut(s) 77
TaaI ACNGT 3 cut(s) 258, 272, 484
TaiI ACGT 2 cut(s) 337, 342
TaqI TCGA 3 cut(s) 34, 127, 177
TasI AATT 6 cut(s) 52, 164, 170, 206, 278, 302
TatI WGTACW 2 cut(s) 258, 477
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 2 cut(s) 92, 429
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 2 cut(s) 91, 489
TseFI GTSAC 2 cut(s) 335, 340
TseI GCWGC 2 cut(s) 291, 647
Tsp45I GTSAC 2 cut(s) 335, 340
TspDTI ATGAA 3 cut(s) 176, 381, 390
TspRI CASTG 2 cut(s) 91, 489
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 3 cut(s) 52, 164, 170
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 2 cut(s) 353, 576
ZraI GACGTC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.