pycom06g09270

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000159
Physical Location & Seq
Reverse (-)
14483136 .. 14484611
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g09270.1

Sequence Viewer

Length: 1476 bp
ATGGTAGTGACCAACTTTGAGTTAAGAATATGGGTTTGCGTCTCAGACGATTTTGATAGTAAAAGATTGCTTCGCGAAATTGTTACTGCGGCAACCAGCCAAAAATGTGGTGACGAAAGTATTGAACAGATGCAAATGAAGTTGCGACGTGCTATGACTGGTAAAAAACTTTTGCTGGTGTTGGTTGATGTCTGGGACAAGGGGCCAATGGGAATAACAGTCAAAAAATGGATTGATTTAAAATCTTTGTTAAATGTTGCAGCTTGCGGGAGTAAAGTTATTGTAACAACGCGCAATGAATCAGTAGCTTTGCTTATGGGGTATGCACACATGCATCTGTTGAAAGTTCTTCCCCTCTCAGATTGCATGACCATATTCATAAAAGTGGCATTTGCAAGAAGAGAAGAGCAAAACCATCCGAACTTGATGAAAATAGGGGAAGATATTGTGAAGAAGTGTGGAGGGGTTCCACTTGCCTTGTATACCCTAGGTGGCCTACTCTGCTCGAACAAGGACGAACGTTATTGGTCACATGTAAGAGATAGTGACATATGGAAATTGGAACAAGGAAGTGACGGCATTTTACCTGCACTCATGTTAAGCTACAATGCGTTGCCAGTTTACTTGAAACCTTGTTTTGCCTTTTGTTCACTGTTCCCCAAGGACTACATATTTCGAAGTTCAGAATTGATCCCGTTGTGGATGGCTCAAGGCTTTGTTCAATCCTCCAGAGGATACCAAGAACTTGAAGATATCGGTCTGGACTACATTAGGAAATTATGTTCTAGATATTTCTTCCAGATTGAGGAAGATAATGTCCTTTTTGTACGGTTTAGAATACACGATCTAGTTCATGATTTAGCAATCTCAACAGCACAGCTAGAATACTCATCTCTAAATTTTCGTCCTGGTGAATCTTCCACGATGATCCGGCATGTGTCAATATCTCAAAAGGAATTGTCCAAGGATAAAGAAGAAGACCCCGAATTCTTACTCCCTTTTGAGAAATTGCGAACCATTCTAATTCCAGACTTGAATAGTGAATATCTTCATACAGAAGTTGGTGTCAACAATCAATCTTTTTTGAAGAGATGCATCTCAATGTTCAACTACCTCCGGGTTCTAGATCTTAGTAACTTGACCCTCGAGTTACTGCCTAGTTCCATCGGTAACTTGAGTCACTTGAGATACCTCGACCTGTCGTATAATCAGCGGATAAAGAAGCTTCCTGATTCCATTTGCGAGCTGCAGCATTTGCAGACCTTACTGCTTATAAACTGTGGAAAAATCAAGGAGTTACCTGAAAACATGGGAAACCTGATTAGCCTCAGGTACTTGGGTTTAACCATAAATCAGCGCCATTTGCCGGAAGTAATTGGACGTCTCACCTCTCTTCGAACTTTATACATCACTGCGTGCAAATATCTTATATCCTTCGGGGAAGGGTGCGAACCCTGCCTAATCTTCGAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

492

Amino Acids

56.34

Weight (kDa)

7.5

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 3 - 134 3.7e-16 NB-ARC domain
WHD_DRP PF23559 218 - 288 6.3e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 366 - 471 1.2e-21 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1274
AatII GACGTC 1 cut(s) 1384
Acc36I ACCTGC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 482
AccII CGCG 2 cut(s) 75, 292
AciI CCGC 3 cut(s) 89, 267, 1213
AclI AACGTT 2 cut(s) 520, 1471
AclWI GGATC 2 cut(s) 685, 922
AcsI RAATTY 2 cut(s) 898, 986
AcyI GRCGYC 1 cut(s) 1381
AdeI CACNNNGTG 1 cut(s) 1416
AfaI GTAC 2 cut(s) 828, 1334
AfiI CCNNNNNNNGG 2 cut(s) 805, 1366
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 8 cut(s) 125, 343, 628, 722, 749, 1036, 1087, 1108
AjiI CACGTC 1 cut(s) 149
AjnI CCWGG 1 cut(s) 907
AloI GAACNNNNNNTCC 2 cut(s) 766, 798
AluBI AGCT 6 cut(s) 263, 308, 603, 880, 1225, 1246
AluI AGCT 6 cut(s) 263, 308, 603, 880, 1225, 1246
Alw26I GTCTC 2 cut(s) 46, 1388
AlwI GGATC 2 cut(s) 685, 922
Ama87I CYCGRG 1 cut(s) 1145
AoxI GGCC 2 cut(s) 203, 493
ApeKI GCWGC 3 cut(s) 260, 1246, 1249
ApoI RAATTY 2 cut(s) 898, 986
Asp700I GAANNNNTTC 1 cut(s) 1047
AspA2I CCTAGG 1 cut(s) 487
AspLEI GCGC 2 cut(s) 294, 1359
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 1118
AsuHPI GGTGA 3 cut(s) 122, 923, 1378
AsuII TTCGAA 3 cut(s) 676, 1396, 1467
AvaI CYCGRG 1 cut(s) 1145
AvrII CCTAGG 1 cut(s) 487
AxyI CCTNAGG 1 cut(s) 1328
BaeI ACNNNNGTAYC 2 cut(s) 1324, 1357
BbsI GAAGAC 1 cut(s) 984
BbvI GCAGC 3 cut(s) 272, 1233, 1261
BccI CCATC 3 cut(s) 423, 697, 1172
BceAI ACGGC 1 cut(s) 592
BciT130I CCWGG 1 cut(s) 909
BciVI GTATCC 1 cut(s) 728
BcnI CCSGG 1 cut(s) 1118
BcoDI GTCTC 2 cut(s) 46, 1388
BfaI CTAG 6 cut(s) 488, 786, 848, 881, 1124, 1158
BfmI CTRYAG 1 cut(s) 1247
BfoI RGCGCY 1 cut(s) 1360
BfuAI ACCTGC 1 cut(s) 595
BfuI GTATCC 1 cut(s) 728
BglII AGATCT 1 cut(s) 1126
BisI GCNGC 4 cut(s) 90, 261, 1247, 1250
BlnI CCTAGG 1 cut(s) 487
BlsI GCNGC 4 cut(s) 91, 262, 1248, 1251
Bme1390I CCNGG 2 cut(s) 909, 1118
BmeT110I CYCGRG 1 cut(s) 1145
BmgBI CACGTC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 204, 468
BmrFI CCNGG 2 cut(s) 909, 1118
BmsI GCATC 4 cut(s) 120, 343, 1082, 1104
BpiI GAAGAC 1 cut(s) 984
BpmI CTGGAG 1 cut(s) 712
Bpu14I TTCGAA 3 cut(s) 676, 1396, 1467
BpuEI CTTGAG 3 cut(s) 693, 1195, 1204
BpuMI CCSGG 1 cut(s) 1118
BsaHI GRCGYC 1 cut(s) 1381
BsaJI CCNNGG 3 cut(s) 487, 660, 963
Bsc4I CCNNNNNNNGG 2 cut(s) 805, 1366
Bse1I ACTGG 2 cut(s) 163, 617
Bse21I CCTNAGG 1 cut(s) 1328
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 909
BseDI CCNNGG 3 cut(s) 487, 660, 963
BseGI GGATG 2 cut(s) 415, 708
BseLI CCNNNNNNNGG 2 cut(s) 805, 1366
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 3 cut(s) 57, 372, 1342
BseNI ACTGG 2 cut(s) 163, 617
BseXI GCAGC 3 cut(s) 272, 1233, 1261
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 2 cut(s) 75, 292
BshFI GGCC 2 cut(s) 205, 495
BsiHKCI CYCGRG 1 cut(s) 1145
BsiSI CCGG 3 cut(s) 931, 1117, 1367
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 805, 1366
BsmAI GTCTC 2 cut(s) 46, 1388
BsmBI CGTCTC 2 cut(s) 46, 1388
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 2 cut(s) 205, 495
BsoBI CYCGRG 1 cut(s) 1145
Bsp119I TTCGAA 3 cut(s) 676, 1396, 1467
Bsp143I GATC 4 cut(s) 690, 844, 927, 1126
Bsp68I TCGCGA 1 cut(s) 75
BspACI CCGC 3 cut(s) 89, 267, 1213
BspANI GGCC 2 cut(s) 205, 495
BspCNI CTCAG 3 cut(s) 56, 371, 1341
BspFNI CGCG 2 cut(s) 75, 292
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 2 cut(s) 204, 468
BspMAI CTGCAG 1 cut(s) 1251
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 2 cut(s) 685, 922
BspQI GCTCTTC 1 cut(s) 399
BspT104I TTCGAA 3 cut(s) 676, 1396, 1467
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 2 cut(s) 163, 617
BssECI CCNNGG 3 cut(s) 487, 660, 963
BssMI GATC 4 cut(s) 690, 844, 927, 1126
BssNAI GTATAC 1 cut(s) 483
BssNI GRCGYC 1 cut(s) 1381
BssT1I CCWWGG 3 cut(s) 487, 660, 963
Bst1107I GTATAC 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 909
Bst4CI ACNGT 4 cut(s) 220, 654, 831, 1280
Bst6I CTCTTC 4 cut(s) 394, 399, 1082, 1398
BstACI GRCGYC 1 cut(s) 1381
BstAPI GCANNNNNTGC 1 cut(s) 1255
BstBI TTCGAA 3 cut(s) 676, 1396, 1467
BstC8I GCNNGC 3 cut(s) 265, 1244, 1417
BstDEI CTNAG 4 cut(s) 43, 358, 1130, 1328
BstF5I GGATG 2 cut(s) 415, 708
BstFNI CGCG 2 cut(s) 75, 292
BstH2I RGCGCY 1 cut(s) 1360
BstHHI GCGC 2 cut(s) 294, 1359
BstKTI GATC 4 cut(s) 693, 847, 930, 1129
BstMAI GTCTC 2 cut(s) 46, 1388
BstMBI GATC 4 cut(s) 690, 844, 927, 1126
BstMWI GCNNNNNNNGC 4 cut(s) 501, 1255, 1363, 1455
BstNI CCWGG 1 cut(s) 909
BstNSI RCATGY 3 cut(s) 334, 536, 938
BstSCI CCNGG 2 cut(s) 907, 1116
BstSFI CTRYAG 1 cut(s) 1247
BstUI CGCG 2 cut(s) 75, 292
BstV1I GCAGC 3 cut(s) 272, 1233, 1261
BstV2I GAAGAC 1 cut(s) 984
BstX2I RGATCY 1 cut(s) 1126
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 1126
BstZ17I GTATAC 1 cut(s) 483
Bsu36I CCTNAGG 1 cut(s) 1328
BsuI GTATCC 1 cut(s) 728
BsuRI GGCC 2 cut(s) 205, 495
BtrI CACGTC 1 cut(s) 149
BtsCI GGATG 2 cut(s) 415, 708
BtsI GCAGTG 1 cut(s) 1410
BtsIMutI CAGTG 2 cut(s) 650, 1410
BtuMI TCGCGA 1 cut(s) 75
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 265, 1244, 1417
CciI TCATGA 1 cut(s) 853
CfoI GCGC 2 cut(s) 294, 1359
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 827, 1333
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 7 cut(s) 331, 367, 533, 595, 854, 935, 1309
CviQI GTAC 2 cut(s) 827, 1333
DdeI CTNAG 4 cut(s) 43, 358, 1130, 1328
DpnI GATC 4 cut(s) 692, 846, 929, 1128
DpnII GATC 4 cut(s) 690, 844, 927, 1126
DraI TTTAAA 1 cut(s) 240
DraIII CACNNNGTG 1 cut(s) 1416
Eam1104I CTCTTC 4 cut(s) 394, 399, 1082, 1398
EarI CTCTTC 4 cut(s) 394, 399, 1082, 1398
Eco130I CCWWGG 3 cut(s) 487, 660, 963
Eco32I GATATC 1 cut(s) 754
Eco81I CCTNAGG 1 cut(s) 1328
Eco88I CYCGRG 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 1 cut(s) 907
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 3 cut(s) 487, 660, 963
EcoT22I ATGCAT 2 cut(s) 336, 1097
ErhI CCWWGG 3 cut(s) 487, 660, 963
Esp3I CGTCTC 2 cut(s) 46, 1388
FaeI CATG 7 cut(s) 334, 370, 536, 598, 857, 938, 1312
FaqI GGGAC 1 cut(s) 209
FatI CATG 7 cut(s) 330, 366, 532, 594, 853, 934, 1308
FauI CCCGC 1 cut(s) 260
FauNDI CATATG 1 cut(s) 551
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 4 cut(s) 90, 261, 1247, 1250
FokI GGATG 2 cut(s) 402, 715
Fsp4HI GCNGC 4 cut(s) 90, 261, 1247, 1250
FspBI CTAG 6 cut(s) 488, 786, 848, 881, 1124, 1158
GlaI GCGC 2 cut(s) 293, 1358
GluI GCNGC 4 cut(s) 90, 261, 1247, 1250
GsuI CTGGAG 1 cut(s) 712
HaeII RGCGCY 1 cut(s) 1360
HaeIII GGCC 2 cut(s) 205, 495
HapII CCGG 3 cut(s) 931, 1117, 1367
HgaI GACGC 1 cut(s) 28
HhaI GCGC 2 cut(s) 294, 1359
Hin1I GRCGYC 1 cut(s) 1381
Hin1II CATG 7 cut(s) 334, 370, 536, 598, 857, 938, 1312
Hin6I GCGC 2 cut(s) 292, 1357
HinP1I GCGC 2 cut(s) 292, 1357
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HindIII AAGCTT 1 cut(s) 1223
HinfI GANTC 4 cut(s) 299, 914, 1177, 1232
HpaII CCGG 3 cut(s) 931, 1117, 1367
HphI GGTGA 3 cut(s) 122, 923, 1378
Hpy166II GTNNAC 4 cut(s) 483, 622, 650, 1069
Hpy188I TCNGA 4 cut(s) 46, 361, 420, 685
Hpy188III TCNNGA 9 cut(s) 74, 729, 761, 786, 799, 854, 1028, 1124, 1229
Hpy8I GTNNAC 4 cut(s) 483, 622, 650, 1069
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 2 cut(s) 1436, 1444
HpyCH4III ACNGT 4 cut(s) 220, 654, 831, 1280
HpyCH4IV ACGT 4 cut(s) 148, 520, 1381, 1471
HpyF10VI GCNNNNNNNGC 4 cut(s) 501, 1255, 1363, 1455
HpyF3I CTNAG 4 cut(s) 43, 358, 1130, 1328
HpySE526I ACGT 4 cut(s) 148, 520, 1381, 1471
Hsp92I GRCGYC 1 cut(s) 1381
Hsp92II CATG 7 cut(s) 334, 370, 536, 598, 857, 938, 1312
HspAI GCGC 2 cut(s) 292, 1357
Kzo9I GATC 4 cut(s) 690, 844, 927, 1126
LguI GCTCTTC 1 cut(s) 399
Lsp1109I GCAGC 3 cut(s) 272, 1233, 1261
LweI GCATC 4 cut(s) 120, 343, 1082, 1104
MaeI CTAG 6 cut(s) 488, 786, 848, 881, 1124, 1158
MaeII ACGT 4 cut(s) 148, 520, 1381, 1471
MalI GATC 4 cut(s) 692, 846, 929, 1128
MboI GATC 4 cut(s) 690, 844, 927, 1126
MflI RGATCY 1 cut(s) 1126
MlyI GAGTC 1 cut(s) 1186
Mph1103I ATGCAT 2 cut(s) 336, 1097
MroXI GAANNNNTTC 1 cut(s) 1047
MseI TTAA 5 cut(s) 23, 239, 251, 599, 1343
MslI CAYNNNNRTG 2 cut(s) 383, 1100
MspA1I CMGCKG 1 cut(s) 1213
MspI CCGG 3 cut(s) 931, 1117, 1367
MspR9I CCNGG 2 cut(s) 909, 1118
MvaI CCWGG 1 cut(s) 909
MvnI CGCG 2 cut(s) 75, 292
MwoI GCNNNNNNNGC 4 cut(s) 501, 1255, 1363, 1455
NciI CCSGG 1 cut(s) 1118
NdeI CATATG 1 cut(s) 551
NdeII GATC 4 cut(s) 690, 844, 927, 1126
NlaIII CATG 7 cut(s) 334, 370, 536, 598, 857, 938, 1312
NlaIV GGNNCC 2 cut(s) 204, 468
NmuCI GTSAC 6 cut(s) 7, 110, 528, 545, 572, 1178
NruI TCGCGA 1 cut(s) 75
NsiI ATGCAT 2 cut(s) 336, 1097
NspI RCATGY 3 cut(s) 334, 536, 938
NspV TTCGAA 3 cut(s) 676, 1396, 1467
PaeR7I CTCGAG 1 cut(s) 1145
PagI TCATGA 1 cut(s) 853
PciI ACATGT 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 3 cut(s) 299, 914, 1232
PkrI GCNGC 4 cut(s) 91, 262, 1248, 1251
PleI GAGTC 1 cut(s) 1185
PpsI GAGTC 1 cut(s) 1185
PscI ACATGT 1 cut(s) 532
PsiI TTATAA 1 cut(s) 1274
Psp1406I AACGTT 2 cut(s) 520, 1471
Psp6I CCWGG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 2 cut(s) 204, 468
PspPI GGNCC 1 cut(s) 203
PspXI VCTCGAGB 1 cut(s) 1145
PstI CTGCAG 1 cut(s) 1251
PsuI RGATCY 1 cut(s) 1126
RruI TCGCGA 1 cut(s) 75
RsaI GTAC 2 cut(s) 828, 1334
RsaNI GTAC 2 cut(s) 827, 1333
RseI CAYNNNNRTG 2 cut(s) 383, 1100
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 5 cut(s) 23, 239, 251, 599, 1343
SatI GCNGC 4 cut(s) 90, 261, 1247, 1250
Sau3AI GATC 4 cut(s) 690, 844, 927, 1126
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 1 cut(s) 1186
ScrFI CCNGG 2 cut(s) 909, 1118
SfaNI GCATC 4 cut(s) 120, 343, 1082, 1104
SfcI CTRYAG 1 cut(s) 1247
Sfr274I CTCGAG 1 cut(s) 1145
SfuI TTCGAA 3 cut(s) 676, 1396, 1467
SlaI CTCGAG 1 cut(s) 1145
SmiMI CAYNNNNRTG 2 cut(s) 383, 1100
SmlI CTYRAG 4 cut(s) 708, 1145, 1174, 1183
SmoI CTYRAG 4 cut(s) 708, 1145, 1174, 1183
SsiI CCGC 3 cut(s) 89, 267, 1213
SspMI CTAG 6 cut(s) 488, 786, 848, 881, 1124, 1158
StyD4I CCNGG 2 cut(s) 907, 1116
StyI CCWWGG 3 cut(s) 487, 660, 963
TaaI ACNGT 4 cut(s) 220, 654, 831, 1280
TaiI ACGT 4 cut(s) 151, 523, 1384, 1474
TaqI TCGA 6 cut(s) 506, 676, 1146, 1194, 1396, 1467
TaqII GACCGA 1 cut(s) 746
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 3 cut(s) 299, 914, 1232
Tru1I TTAA 5 cut(s) 23, 239, 251, 599, 1343
Tru9I TTAA 5 cut(s) 23, 239, 251, 599, 1343
TscAI CASTG 2 cut(s) 657, 1417
TseFI GTSAC 6 cut(s) 7, 110, 528, 545, 572, 1178
TseI GCWGC 3 cut(s) 260, 1246, 1249
Tsp45I GTSAC 6 cut(s) 7, 110, 528, 545, 572, 1178
TspDTI ATGAA 6 cut(s) 152, 312, 367, 443, 842, 1040
TspRI CASTG 2 cut(s) 657, 1417
XapI RAATTY 2 cut(s) 898, 986
XbaI TCTAGA 2 cut(s) 785, 1123
XceI RCATGY 3 cut(s) 334, 536, 938
XhoI CTCGAG 1 cut(s) 1145
XmaJI CCTAGG 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 1047
XspI CTAG 6 cut(s) 488, 786, 848, 881, 1124, 1158
ZraI GACGTC 1 cut(s) 1382
Zsp2I ATGCAT 2 cut(s) 336, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.