FvH4_5g16900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000159
Physical Location & Seq
Forward (+)
9650728 .. 9653960
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16900.t1

Sequence Viewer

Length: 2607 bp
ATGGGGGAATTCGTCACAAGCATTGCAGAGACCGCAATGATTGGGCTAGCTTCTTATACATACAAAGAAGTTTGCTTGGCAAGAGGTGTCACAGATGAGCTAGCCAAGCTCAAGGAGACCTTCTCAGATGTCAAAAAAGTGGTCCTTGATGCAGAGGAAAAGCAGAGGAATGATCCCCGTCTGACCGAGTGGTTAGGGAAGCTCAAAGATGTATGCTACGACATCGATGATGTGCTTGATGAATTTGCATTTACAGAGCTACAAATGCAAGTGATGAACAACCGCGGAAGTGTGTTAACACTAAAGGTACGCACATTCTTTTCGCGTTGGAACCCACTTGTGTTTAACATCAAAATGGCTCATAGAATCAAAGGGATTAGAGAAGAGTTAGATAGAGTGGCAGCTGAAAAAGCTTTGTTTCCTCTTGAGGAACGAGCAGGAGATTGGCAGGGGGTGCACGTAAAGAGGGAGACTCACTCTTTTGTGCAAGTCTATGATGTCATTGGGAGAGATGATGATAAAAAACAGATTATCATTCATCTCTTGAACTTAAGATTGAAAGATCCTGGTGTTCGTGACACCGTTTCTGTTATTTCCATCTTGGGCTTAGGAGGTATGGGAAAGACCACCCTTGCCAAGTTAGTGTACAATGATAAAAGGGTAGTGAAAAACTTTGAGTTGAGAATGTGGGTGTATGTCTCGGACCACTTTGATATCAAAAGATTGGTTCGAGATATTATCACTTCTGCAACTAACCAAAACTGTGGTGATGATGAAAGTCTAGAACAGTTGCAAAAGAAGTTGCAAAATGTTCTAACTGATAAACATTTTTTGTTAGTGTTGGATGATGTTTGGGACAAGAGTGAAATTGGTGTAACAATTTCAAAATGGCTTGAATTAAAAGCTCTTTTGAATGTTGGAGCCAATGAGAGTAAAATCATTGTCACAACACGCAATGAGTCAGTTACTAAGGTTATGGAATCTGTATACGTGCATCCATTGAAAGGTCTTCGCCACGAAGATTGTAAATCCTTGTTCCTCCAAAGAGCATTTGCCCGAAGGGGGGAAGAGGAACGATATCCACAACTAGTGGAAATCGGAGACGATATTGCAGAGAAGTGTGGAGGGGTTCCTCTAGCCGTAACTACCCTAGGGAGCATGCTATGTTTAAAAAGAGAGCAGCATGCTTGGTCCGAAGTAAGAGATAGCGACATTTGGAAGCTGAAACAGGAGCACAACGACATATCCCCTGCCCTCAAATTGAGCTATGATGCATTGCCGCCCTACTTGAAACCATGCTTTGCCTTTTGCTCAATTTTCCCCAAAGACTACATATTCCGTAGTACAGATTTGGTCCCCCTCTGGATGGCACAAGGCTTTCTTCAGTCCTCAACAGGAAGCCAAGAGCCTGAAGAGATGGGACTAGACTACATAAGGCAGATATGTTCTAGATCATTGTTTCAAATGCATGAAGATTCAATTCACTCCATTGTCTTTGAAATGCATCATCTAGTTCATGACTTGGCCATCTCGGTGGCACAAGTAGAGTGTTCATCTGAAAATTTTCGTCCTCCTTCTACCTCCATTTCGCACAAGAAAGTTCGACATGTGTCAATCTCGCGAGATGACTTGTGCATGAAGGAAGTACGAGTTCCTGATTACTTGCTTCAGCTGAAGAAAGTGTGGACCATTCTATTTCCAAATCCAGGAGATACGGTCATCACCAACAAACCTTTGTTGAAGACATGCTTCAAGAGATTCAAGTACCTGCGCGTGCTAGATTTGAGTGGTTGGAGTCTTGAAAAGTTACCAAGTTCAATTGGTAATTTGAGTCACTTGAGATATCTAGACTTGTCTAGGAATCCAAGCCTGCAAAAGCTTCCGGATTCCATTTGCGAGTTGCAAAATTTGCAAACATTGCTACTTTCAGGCTGTGAGAATCTCAAAGGCTTGCCGAAAGACATAGGGAGCTTGATCAACCTCAGAAAGTTGGTGTTCACCACAAGTCGGATGGATTTGCCCGAAGAAATCAAAAGCCTCACTTCTCTTCGACATTTCGAGGTCCACAGCTGCAGTAATCTTGAATCCTTAGGTGAAGTGGTACAATCTCTCACTAACCTTAGAATTCTTGTCATTAGCAATTGTGGAAATCTAGAGTCACTGCCACAGTTGAGTCCATCACTAAAGGCCTTAGTCATCTCTGACTGTGAGAAGCTCGACTTGATGAGTTTGGGAGAAGGCATTGAAGGTCTTCTATCATTTTCCATCTCCAAAATGTCAAATTTAGAGGCTTTGCCCCATTGGCTTCAAGACTCCAAACCCAGTCTACAAAGCATACGCATAGAAAATTGCGTGCATCTCAAGGTACTTCCGGAGTGGCTGCAAAGCTTGATGAAGCTGCAACAACTGGTCATAGAAGGTTGTCCTGAATTGTTGGCTTTGCCGGAGGGGATGCATCATCTCTTTGCCTTGAAAGAATTGAAGATCAATGACTGTTCTAAATTGGGAGAAAGTTGCAGAAGGAAAGAAGGTCAGGACTGGTCCAAGATCACTCGTGTTGCCAAGGTTACAATTGATGGAGAGAACATTGCATCAAGTGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

869

Amino Acids

98.69

Weight (kDa)

6.28

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 16 - 94 2.3e-19 Rx N-terminal domain
NB-ARC PF00931 193 - 353 8e-34 NB-ARC domain
WHD_DRP PF23559 439 - 509 5.3e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 582 - 805 1.4e-26 Leucine-rich repeat region
LRR_8 PF13855 589 - 645 1.5e-07 Leucine rich repeat
LRR_13 PF23286 599 - 743 6.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_RPS2 PF23247 677 - 808 1.5e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1776
AccI GTMKAC 2 cut(s) 987, 2326
AccII CGCG 4 cut(s) 285, 325, 1621, 1773
AccIII TCCGGA 2 cut(s) 1882, 2371
AciI CCGC 4 cut(s) 33, 283, 285, 1280
AclWI GGATC 2 cut(s) 167, 557
AcoI YGGCCR 1 cut(s) 1524
AcsI RAATTY 6 cut(s) 8, 242, 1561, 1906, 2124, 2281
AcuI CTGAAG 4 cut(s) 1367, 1431, 1652, 1694
AfaI GTAC 7 cut(s) 309, 647, 1345, 1647, 1766, 2103, 2367
AfiI CCNNNNNNNGG 6 cut(s) 1004, 1062, 1063, 1366, 1706, 2007
AflII CTTAAG 1 cut(s) 550
AflIII ACRYGT 1 cut(s) 1606
AhlI ACTAGT 1 cut(s) 1087
AjnI CCWGG 2 cut(s) 565, 1705
Alw21I GWGCWC 2 cut(s) 459, 1236
Alw26I GTCTC 5 cut(s) 23, 110, 464, 703, 1095
Alw44I GTGCAC 1 cut(s) 455
AlwI GGATC 2 cut(s) 167, 557
Aor13HI TCCGGA 2 cut(s) 1882, 2371
AoxI GGCC 2 cut(s) 1524, 2187
ApaLI GTGCAC 1 cut(s) 455
ApeKI GCWGC 5 cut(s) 401, 1180, 2070, 2380, 2398
ApoI RAATTY 6 cut(s) 8, 242, 1561, 1906, 2124, 2281
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 2 cut(s) 1563, 2250
AspA2I CCTAGG 1 cut(s) 1150
AspLEI GCGC 1 cut(s) 1773
AspS9I GGNCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
AsuHPI GGTGA 4 cut(s) 779, 1714, 1990, 2105
AsuNHI GCTAGC 2 cut(s) 46, 100
AvaII GGWCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
AvrII CCTAGG 1 cut(s) 1150
AxyI CCTNAGG 1 cut(s) 2089
BaeGI GKGCMC 1 cut(s) 459
BalI TGGCCA 1 cut(s) 1526
BauI CACGAG 1 cut(s) 2553
BbsI GAAGAC 3 cut(s) 1001, 1748, 2243
Bbv12I GWGCWC 2 cut(s) 459, 1236
BbvI GCAGC 5 cut(s) 413, 1192, 2057, 2367, 2385
BccI CCATC 8 cut(s) 605, 1360, 1411, 1535, 2005, 2185, 2273, 2570
BceAI ACGGC 1 cut(s) 1124
BciT130I CCWGG 2 cut(s) 567, 1707
BclI TGATCA 1 cut(s) 1974
BcoDI GTCTC 5 cut(s) 23, 110, 464, 703, 1095
BcuI ACTAGT 1 cut(s) 1087
BfmI CTRYAG 1 cut(s) 2071
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 1776
BglI GCCNNNNNGGC 1 cut(s) 2302
BisI GCNGC 6 cut(s) 402, 1181, 1280, 2071, 2381, 2399
BlnI CCTAGG 1 cut(s) 1150
BlsI GCNGC 6 cut(s) 403, 1182, 1281, 2072, 2382, 2400
Bme1390I CCNGG 2 cut(s) 567, 1707
Bme18I GGWCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
BmgT120I GGNCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
BmiI GGNNCC 4 cut(s) 332, 922, 1131, 1356
BmrFI CCNGG 2 cut(s) 567, 1707
BmrI ACTGGG 1 cut(s) 2316
BmsI GCATC 8 cut(s) 139, 1003, 1261, 1513, 2365, 2442, 2464, 2600
BmtI GCTAGC 2 cut(s) 50, 104
BmuI ACTGGG 1 cut(s) 2316
BoxI GACNNNNGTC 1 cut(s) 1609
BpiI GAAGAC 3 cut(s) 1001, 1748, 2243
BplI GAGNNNNNCTC 6 cut(s) 107, 139, 457, 489, 461, 493
Bpu10I CCTNAGC 1 cut(s) 607
BpuEI CTTGAG 4 cut(s) 95, 446, 1858, 2345
Bsa29I ATCGAT 1 cut(s) 225
BsaAI YACGTR 2 cut(s) 460, 991
BsaI GGTCTC 2 cut(s) 23, 110
BsaJI CCNNGG 3 cut(s) 283, 1150, 2562
BsaWI WCCGGW 2 cut(s) 1882, 2371
BsaXI ACNNNNNCTCC 2 cut(s) 1701, 1731
Bsc4I CCNNNNNNNGG 6 cut(s) 1004, 1062, 1063, 1366, 1706, 2007
Bse1I ACTGG 3 cut(s) 2322, 2412, 2543
Bse21I CCTNAGG 1 cut(s) 2089
Bse3DI GCAATG 6 cut(s) 21, 42, 961, 1274, 1916, 2586
BseAI TCCGGA 2 cut(s) 1882, 2371
BseBI CCWGG 2 cut(s) 567, 1707
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 283, 1150, 2562
BseGI GGATG 5 cut(s) 850, 994, 1371, 2016, 2457
BseLI CCNNNNNNNGG 6 cut(s) 1004, 1062, 1063, 1366, 1706, 2007
BseMI GCAATG 6 cut(s) 21, 42, 961, 1274, 1916, 2586
BseMII CTCAG 2 cut(s) 138, 1996
BseNI ACTGG 3 cut(s) 2322, 2412, 2543
BseSI GKGCMC 1 cut(s) 459
BseXI GCAGC 5 cut(s) 413, 1192, 2057, 2367, 2385
Bsh1236I CGCG 4 cut(s) 285, 325, 1621, 1773
BshFI GGCC 2 cut(s) 1526, 2189
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 459, 1236
BsiSI CCGG 3 cut(s) 1883, 2372, 2444
BslFI GGGAC 3 cut(s) 869, 1340, 1434
BslI CCNNNNNNNGG 6 cut(s) 1004, 1062, 1063, 1366, 1706, 2007
BsmAI GTCTC 5 cut(s) 23, 110, 464, 703, 1095
BsmBI CGTCTC 1 cut(s) 1095
BsmFI GGGAC 3 cut(s) 869, 1340, 1434
BsnI GGCC 2 cut(s) 1526, 2189
Bso31I GGTCTC 2 cut(s) 23, 110
Bsp1286I GDGCHC 2 cut(s) 459, 1236
Bsp13I TCCGGA 2 cut(s) 1882, 2371
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
Bsp68I TCGCGA 1 cut(s) 1621
BspACI CCGC 4 cut(s) 33, 283, 285, 1280
BspANI GGCC 2 cut(s) 1526, 2189
BspCNI CTCAG 2 cut(s) 137, 1995
BspDI ATCGAT 1 cut(s) 225
BspEI TCCGGA 2 cut(s) 1882, 2371
BspFNI CGCG 4 cut(s) 285, 325, 1621, 1773
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 4 cut(s) 332, 922, 1131, 1356
BspMAI CTGCAG 1 cut(s) 2075
BspMI ACCTGC 1 cut(s) 1776
BspOI GCTAGC 2 cut(s) 50, 104
BspPI GGATC 2 cut(s) 167, 557
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 2 cut(s) 23, 110
BsrDI GCAATG 6 cut(s) 21, 42, 961, 1274, 1916, 2586
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 3 cut(s) 2322, 2412, 2543
BssECI CCNNGG 3 cut(s) 283, 1150, 2562
BssMI GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
BssNAI GTATAC 1 cut(s) 988
BssSI CACGAG 1 cut(s) 2553
BssT1I CCWWGG 2 cut(s) 1150, 2562
Bst1107I GTATAC 1 cut(s) 988
Bst2BI CACGAG 1 cut(s) 2553
Bst2UI CCWGG 2 cut(s) 567, 1707
Bst4CI ACNGT 7 cut(s) 583, 764, 789, 1717, 2169, 2207, 2495
Bst6I CTCTTC 4 cut(s) 378, 1062, 1407, 2052
BstAFI CTTAAG 1 cut(s) 550
BstAPI GCANNNNNTGC 3 cut(s) 454, 1909, 1918
BstAUI TGTACA 1 cut(s) 645
BstBAI YACGTR 2 cut(s) 460, 991
BstC8I GCNNGC 8 cut(s) 48, 102, 1160, 1185, 1775, 1871, 1952, 2354
BstDEI CTNAG 7 cut(s) 124, 607, 969, 1982, 2089, 2120, 2191
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 5 cut(s) 850, 994, 1371, 2016, 2457
BstFNI CGCG 4 cut(s) 285, 325, 1621, 1773
BstHHI GCGC 1 cut(s) 1773
BstKTI GATC 6 cut(s) 175, 565, 1454, 1977, 2487, 2550
BstMAI GTCTC 5 cut(s) 23, 110, 464, 703, 1095
BstMBI GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
BstMWI GCNNNNNNNGC 8 cut(s) 32, 106, 265, 410, 454, 1909, 1918, 2302
BstNI CCWGG 2 cut(s) 567, 1707
BstNSI RCATGY 4 cut(s) 1162, 1187, 1610, 1749
BstPAI GACNNNNGTC 1 cut(s) 1609
BstSCI CCNGG 2 cut(s) 565, 1705
BstSFI CTRYAG 1 cut(s) 2071
BstSLI GKGCMC 1 cut(s) 459
BstUI CGCG 4 cut(s) 285, 325, 1621, 1773
BstV1I GCAGC 5 cut(s) 413, 1192, 2057, 2367, 2385
BstV2I GAAGAC 3 cut(s) 1001, 1748, 2243
BstX2I RGATCY 1 cut(s) 562
BstXI CCANNNNNNTGG 2 cut(s) 764, 1534
BstYI RGATCY 1 cut(s) 562
BstZ17I GTATAC 1 cut(s) 988
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 2089
BsuRI GGCC 2 cut(s) 1526, 2189
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 5 cut(s) 850, 994, 1371, 2016, 2457
BtsI GCAGTG 1 cut(s) 2159
BtsIMutI CAGTG 1 cut(s) 2159
BtuMI TCGCGA 1 cut(s) 1621
BveI ACCTGC 1 cut(s) 1776
Cac8I GCNNGC 8 cut(s) 48, 102, 1160, 1185, 1775, 1871, 1952, 2354
CciI TCATGA 1 cut(s) 1516
CfoI GCGC 1 cut(s) 1773
Cfr13I GGNCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
Cfr42I CCGCGG 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 225
Csp6I GTAC 7 cut(s) 308, 646, 1344, 1646, 1765, 2102, 2366
CspCI CAANNNNNGTGG 2 cut(s) 1004, 1039
CviAII CATG 8 cut(s) 1159, 1184, 1296, 1469, 1517, 1607, 1636, 1746
CviQI GTAC 7 cut(s) 308, 646, 1344, 1646, 1765, 2102, 2366
DdeI CTNAG 7 cut(s) 124, 607, 969, 1982, 2089, 2120, 2191
DpnI GATC 6 cut(s) 174, 564, 1453, 1976, 2486, 2549
DpnII GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
DraI TTTAAA 1 cut(s) 1170
EaeI YGGCCR 1 cut(s) 1524
Eam1104I CTCTTC 4 cut(s) 378, 1062, 1407, 2052
EarI CTCTTC 4 cut(s) 378, 1062, 1407, 2052
Eco130I CCWWGG 2 cut(s) 1150, 2562
Eco147I AGGCCT 1 cut(s) 2189
Eco31I GGTCTC 2 cut(s) 23, 110
Eco32I GATATC 3 cut(s) 715, 1079, 1844
Eco47I GGWCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
Eco57I CTGAAG 4 cut(s) 1367, 1431, 1652, 1694
Eco81I CCTNAGG 1 cut(s) 2089
EcoRI GAATTC 2 cut(s) 8, 2124
EcoRII CCWGG 2 cut(s) 565, 1705
EcoRV GATATC 3 cut(s) 715, 1079, 1844
EcoT14I CCWWGG 2 cut(s) 1150, 2562
EcoT22I ATGCAT 4 cut(s) 1276, 1470, 1506, 2457
ErhI CCWWGG 2 cut(s) 1150, 2562
Esp3I CGTCTC 1 cut(s) 1095
FaeI CATG 8 cut(s) 1162, 1187, 1299, 1472, 1520, 1610, 1639, 1749
FaqI GGGAC 3 cut(s) 869, 1340, 1434
FatI CATG 8 cut(s) 1158, 1183, 1295, 1468, 1516, 1606, 1635, 1745
FbaI TGATCA 1 cut(s) 1974
FblI GTMKAC 2 cut(s) 987, 2326
Fnu4HI GCNGC 6 cut(s) 402, 1181, 1280, 2071, 2381, 2399
FokI GGATG 5 cut(s) 857, 981, 1378, 2023, 2464
Fsp4HI GCNGC 6 cut(s) 402, 1181, 1280, 2071, 2381, 2399
GlaI GCGC 1 cut(s) 1772
GluI GCNGC 6 cut(s) 402, 1181, 1280, 2071, 2381, 2399
HaeIII GGCC 2 cut(s) 1526, 2189
HapII CCGG 3 cut(s) 1883, 2372, 2444
HhaI GCGC 1 cut(s) 1773
Hin1II CATG 8 cut(s) 1162, 1187, 1299, 1472, 1520, 1610, 1639, 1749
Hin6I GCGC 1 cut(s) 1771
HinP1I GCGC 1 cut(s) 1771
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 3 cut(s) 411, 1877, 2386
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 3 cut(s) 1883, 2372, 2444
HphI GGTGA 4 cut(s) 779, 1714, 1990, 2105
Hpy166II GTNNAC 8 cut(s) 297, 457, 646, 988, 1686, 1998, 2065, 2327
Hpy188I TCNGA 9 cut(s) 127, 183, 703, 1100, 1195, 1558, 1985, 2010, 2203
Hpy8I GTNNAC 8 cut(s) 297, 457, 646, 988, 1686, 1998, 2065, 2327
HpyAV CCTTC 9 cut(s) 130, 1053, 1584, 1633, 2231, 2240, 2411, 2514, 2522
HpyCH4III ACNGT 7 cut(s) 583, 764, 789, 1717, 2169, 2207, 2495
HpyCH4IV ACGT 2 cut(s) 459, 990
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 106, 265, 410, 454, 1909, 1918, 2302
HpyF3I CTNAG 7 cut(s) 124, 607, 969, 1982, 2089, 2120, 2191
HpySE526I ACGT 2 cut(s) 459, 990
Hsp92II CATG 8 cut(s) 1162, 1187, 1299, 1472, 1520, 1610, 1639, 1749
HspAI GCGC 1 cut(s) 1771
Kpn2I TCCGGA 2 cut(s) 1882, 2371
Ksp22I TGATCA 1 cut(s) 1974
KspAI GTTAAC 1 cut(s) 297
KspI CCGCGG 1 cut(s) 286
Kzo9I GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
LmnI GCTCC 4 cut(s) 920, 1155, 1231, 1968
Lsp1109I GCAGC 5 cut(s) 413, 1192, 2057, 2367, 2385
LweI GCATC 8 cut(s) 139, 1003, 1261, 1513, 2365, 2442, 2464, 2600
MaeII ACGT 2 cut(s) 459, 990
MalI GATC 6 cut(s) 174, 564, 1453, 1976, 2486, 2549
MboI GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
MfeI CAATTG 3 cut(s) 1818, 2140, 2571
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 2 cut(s) 459, 1236
MlsI TGGCCA 1 cut(s) 1526
MluNI TGGCCA 1 cut(s) 1526
MlyI GAGTC 7 cut(s) 466, 968, 1804, 1840, 2165, 2182, 2306
MmeI TCCRAC 5 cut(s) 308, 822, 898, 1772, 1988
Mox20I TGGCCA 1 cut(s) 1526
Mph1103I ATGCAT 4 cut(s) 1276, 1470, 1506, 2457
MroI TCCGGA 2 cut(s) 1882, 2371
MroXI GAANNNNTTC 2 cut(s) 1563, 2250
MscI TGGCCA 1 cut(s) 1526
MseI TTAA 5 cut(s) 296, 345, 551, 899, 1169
MslI CAYNNNNRTG 2 cut(s) 353, 1532
Msp20I TGGCCA 1 cut(s) 1526
MspA1I CMGCKG 4 cut(s) 285, 404, 1672, 2070
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 3 cut(s) 1883, 2372, 2444
MspR9I CCNGG 2 cut(s) 567, 1707
MunI CAATTG 3 cut(s) 1818, 2140, 2571
MvaI CCWGG 2 cut(s) 567, 1707
MvnI CGCG 4 cut(s) 285, 325, 1621, 1773
MwoI GCNNNNNNNGC 8 cut(s) 32, 106, 265, 410, 454, 1909, 1918, 2302
NdeII GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
NheI GCTAGC 2 cut(s) 46, 100
NlaIII CATG 8 cut(s) 1162, 1187, 1299, 1472, 1520, 1610, 1639, 1749
NlaIV GGNNCC 4 cut(s) 332, 922, 1131, 1356
NmuCI GTSAC 6 cut(s) 13, 88, 575, 943, 1832, 2157
NruI TCGCGA 1 cut(s) 1621
NsiI ATGCAT 4 cut(s) 1276, 1470, 1506, 2457
NspI RCATGY 4 cut(s) 1162, 1187, 1610, 1749
PaeI GCATGC 2 cut(s) 1162, 1187
PagI TCATGA 1 cut(s) 1516
PceI AGGCCT 1 cut(s) 2189
PciI ACATGT 1 cut(s) 1606
PdmI GAANNNNTTC 2 cut(s) 1563, 2250
PfeI GAWTC 8 cut(s) 366, 980, 1475, 1758, 1861, 1886, 1939, 2084
PfoI TCCNGGA 1 cut(s) 1705
PkrI GCNGC 6 cut(s) 403, 1182, 1281, 2072, 2382, 2400
PleI GAGTC 7 cut(s) 466, 967, 1803, 1839, 2164, 2181, 2306
PpsI GAGTC 7 cut(s) 466, 967, 1803, 1839, 2164, 2181, 2306
Ppu21I YACGTR 2 cut(s) 460, 991
PscI ACATGT 1 cut(s) 1606
PshAI GACNNNNGTC 1 cut(s) 1609
Psp6I CCWGG 2 cut(s) 565, 1705
PspGI CCWGG 2 cut(s) 565, 1705
PspN4I GGNNCC 4 cut(s) 332, 922, 1131, 1356
PspPI GGNCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
PstI CTGCAG 1 cut(s) 2075
PsuI RGATCY 1 cut(s) 562
PvuII CAGCTG 3 cut(s) 404, 1672, 2070
RruI TCGCGA 1 cut(s) 1621
RsaI GTAC 7 cut(s) 309, 647, 1345, 1647, 1766, 2103, 2367
RsaNI GTAC 7 cut(s) 308, 646, 1344, 1646, 1765, 2102, 2366
RseI CAYNNNNRTG 2 cut(s) 353, 1532
SacII CCGCGG 1 cut(s) 286
SaqAI TTAA 5 cut(s) 296, 345, 551, 899, 1169
SatI GCNGC 6 cut(s) 402, 1181, 1280, 2071, 2381, 2399
Sau3AI GATC 6 cut(s) 172, 562, 1451, 1974, 2484, 2547
Sau96I GGNCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
SchI GAGTC 7 cut(s) 466, 968, 1804, 1840, 2165, 2182, 2306
ScrFI CCNGG 2 cut(s) 567, 1707
SduI GDGCHC 2 cut(s) 459, 1236
SfaNI GCATC 8 cut(s) 139, 1003, 1261, 1513, 2365, 2442, 2464, 2600
SfcI CTRYAG 1 cut(s) 2071
Sfr303I CCGCGG 1 cut(s) 286
SgrBI CCGCGG 1 cut(s) 286
SinI GGWCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
SmiMI CAYNNNNRTG 2 cut(s) 353, 1532
SmlI CTYRAG 5 cut(s) 110, 425, 550, 1837, 2360
SmoI CTYRAG 5 cut(s) 110, 425, 550, 1837, 2360
SpeI ACTAGT 1 cut(s) 1087
SphI GCATGC 2 cut(s) 1162, 1187
SseBI AGGCCT 1 cut(s) 2189
SsiI CCGC 4 cut(s) 33, 283, 285, 1280
StuI AGGCCT 1 cut(s) 2189
StyD4I CCNGG 2 cut(s) 565, 1705
StyI CCWWGG 2 cut(s) 1150, 2562
TaaI ACNGT 7 cut(s) 583, 764, 789, 1717, 2169, 2207, 2495
TaiI ACGT 2 cut(s) 462, 993
TaqI TCGA 6 cut(s) 225, 730, 1603, 2050, 2058, 2217
TaqII GACCGA 1 cut(s) 200
TatI WGTACW 2 cut(s) 645, 1343
TauI GCSGC 1 cut(s) 1282
TfiI GAWTC 8 cut(s) 366, 980, 1475, 1758, 1861, 1886, 1939, 2084
Tru1I TTAA 5 cut(s) 296, 345, 551, 899, 1169
Tru9I TTAA 5 cut(s) 296, 345, 551, 899, 1169
TscAI CASTG 1 cut(s) 2166
TseFI GTSAC 6 cut(s) 13, 88, 575, 943, 1832, 2157
TseI GCWGC 5 cut(s) 401, 1180, 2070, 2380, 2398
Tsp45I GTSAC 6 cut(s) 13, 88, 575, 943, 1832, 2157
TspDTI ATGAA 9 cut(s) 255, 290, 527, 789, 1485, 1505, 1542, 1652, 2408
TspGWI ACGGA 1 cut(s) 1328
TspRI CASTG 1 cut(s) 2166
Vha464I CTTAAG 1 cut(s) 550
VneI GTGCAC 1 cut(s) 455
VpaK11BI GGWCC 7 cut(s) 142, 703, 1191, 1354, 1686, 2062, 2541
XapI RAATTY 6 cut(s) 8, 242, 1561, 1906, 2124, 2281
XbaI TCTAGA 4 cut(s) 781, 1448, 1846, 2152
XceI RCATGY 4 cut(s) 1162, 1187, 1610, 1749
XcmI CCANNNNNNNNNTGG 1 cut(s) 2008
XmaJI CCTAGG 1 cut(s) 1150
XmiI GTMKAC 2 cut(s) 987, 2326
XmnI GAANNNNTTC 2 cut(s) 1563, 2250
Zsp2I ATGCAT 4 cut(s) 1276, 1470, 1506, 2457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.