RchiOBHm_Chr7g0179121

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000159
Physical Location & Seq
Reverse (-)
1511493 .. 1521378
9886 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15964

Sequence Viewer

Length: 966 bp
ATGGAGTTTGCCTCTGGCATTGCTGAGACCGTCTCAGTTAGGCTAGCCTCCTATGCTGCCCAAGAAGTTTGCTTGGCTTGGGGTGCCCAACATGAGCTCAGGAAGCTCAATAAGACCTTGTTAGCCATCAAACTTGTGCTCGAGGATGCAGAGAAGAAGCAATTGCAGAATCCCCTCATCACTCATTGGTTAGGAAACCTCAAAGATGTTTGCAATGATGCCGAGGATGTACTGGACGAATTTGAGTTCCGAAAGCTCCGCCTCAAAGTGCAACGGAGCGTCAAAGGAAAGTTCTTAAGCCTTCAAGGTTGTGAGGCACTTGAGGAGTTACCCAACGGCATAGGAAACCTGATCAACCTCAGACTCTTGTTCTTAACTACACAGCAGATGTATTTGCCGAAAGGAGTATTCAAACGCCTCACCTTGCTTCAAACATTATTAATCTCGAGCTGTAAAAATCTAAAATCTCTGGGTGAAGAGATGGAATACCTCACTAACCTCCGTCAATTGTGGATTGGTATGTGTGGGAATCTGGAATCCTTGCCACCTAACATGAAGAACTTGACTGCATTCCATACTTTGGCGATCGCTGATTGTAAGAAGCTTGAGTTAATGAGGTCGGGGGAAGGCATCAGTGGTCTCAGATCATTCTCTGTCGCCGAATCAAATTTGGAGGCTTTGCCCAATTGGCTTCAAGAGTCCGTAGACACTTTACAAAGCATGCTAATCGGAGATTGTGATAATCTCACGGCACTTCCAGACTGGTTACAAAATCTCACCTTGCTGGAGCAACTGGTGATTGGGGAATGCCCCAAATTGTCTGCTTTGCCGCAAGGGATGCATTGCCTCACTGCCTTGAGAGTTTTGATGATTAGCGGGTGTCCTGAATTGAGAAAAAGATGCAAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAACATGTCCCAAAAAGGGTCCCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

321

Amino Acids

36.1

Weight (kDa)

7.5

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 92 2.6e-21 Rx N-terminal domain
LRR_14 PF23598 99 - 241 2.3e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 580
AccI GTMKAC 1 cut(s) 705
AciI CCGC 3 cut(s) 259, 830, 876
AcsI RAATTY 2 cut(s) 239, 667
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 580, 952
AflII CTTAAG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 940
AgsI TTSAA 4 cut(s) 305, 412, 431, 695
AluBI AGCT 5 cut(s) 97, 106, 256, 450, 604
AluI AGCT 5 cut(s) 97, 106, 256, 450, 604
Alw21I GWGCWC 2 cut(s) 99, 141
Alw26I GTCTC 3 cut(s) 20, 37, 644
Ama87I CYCGRG 2 cut(s) 140, 445
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 2 cut(s) 239, 667
AseI ATTAAT 1 cut(s) 440
AsiSI GCGATCGC 1 cut(s) 588
AspS9I GGNCC 2 cut(s) 927, 955
AsuHPI GGTGA 4 cut(s) 412, 485, 769, 808
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 140, 445
AvaII GGWCC 2 cut(s) 927, 955
BaeGI GKGCMC 1 cut(s) 88
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 99, 141
BbvI GCAGC 1 cut(s) 43
BccI CCATC 2 cut(s) 134, 475
BceAI ACGGC 2 cut(s) 352, 765
BcgI CGANNNNNNTGC 2 cut(s) 709, 743
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 20, 37, 644
BfaI CTAG 1 cut(s) 44
BfrI CTTAAG 1 cut(s) 295
BglI GCCNNNNNGGC 1 cut(s) 688
BisI GCNGC 2 cut(s) 57, 830
BlsI GCNGC 2 cut(s) 58, 831
Bme18I GGWCC 2 cut(s) 927, 955
BmeT110I CYCGRG 2 cut(s) 140, 445
BmgT120I GGNCC 2 cut(s) 927, 955
BmiI GGNNCC 3 cut(s) 85, 956, 957
BmsI GCATC 5 cut(s) 136, 208, 639, 828, 890
BmtI GCTAGC 1 cut(s) 47
BplI GAGNNNNNCTC 3 cut(s) 28, 17, 49
BpmI CTGGAG 1 cut(s) 806
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 3 cut(s) 341, 626, 877
BsaI GGTCTC 2 cut(s) 20, 644
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 952
Bse1I ACTGG 3 cut(s) 237, 767, 798
Bse3DI GCAATG 3 cut(s) 18, 220, 841
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 151, 232, 843
BseLI CCNNNNNNNGG 2 cut(s) 580, 952
BseMI GCAATG 3 cut(s) 18, 220, 841
BseMII CTCAG 5 cut(s) 15, 48, 112, 373, 655
BseNI ACTGG 3 cut(s) 237, 767, 798
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 1 cut(s) 43
Bsh1285I CGRYCG 1 cut(s) 588
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 99, 141
BsiHKCI CYCGRG 2 cut(s) 140, 445
BslFI GGGAC 2 cut(s) 929, 941
BslI CCNNNNNNNGG 2 cut(s) 580, 952
BsmAI GTCTC 3 cut(s) 20, 37, 644
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 929, 941
BsmI GAATGC 2 cut(s) 569, 812
Bso31I GGTCTC 2 cut(s) 20, 644
BsoBI CYCGRG 2 cut(s) 140, 445
Bsp1286I GDGCHC 3 cut(s) 88, 99, 141
Bsp143I GATC 4 cut(s) 351, 585, 644, 933
BspACI CCGC 3 cut(s) 259, 830, 876
BspCNI CTCAG 5 cut(s) 16, 47, 111, 372, 654
BspLI GGNNCC 3 cut(s) 85, 956, 957
BspOI GCTAGC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 295
BspTNI GGTCTC 2 cut(s) 20, 644
BsrDI GCAATG 3 cut(s) 18, 220, 841
BsrI ACTGG 3 cut(s) 237, 767, 798
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 351, 585, 644, 933
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 471
BstAFI CTTAAG 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 838
BstC8I GCNNGC 2 cut(s) 45, 722
BstDEI CTNAG 5 cut(s) 24, 34, 98, 359, 641
BstF5I GGATG 3 cut(s) 151, 232, 843
BstKTI GATC 4 cut(s) 354, 588, 647, 936
BstMAI GTCTC 3 cut(s) 20, 37, 644
BstMBI GATC 4 cut(s) 351, 585, 644, 933
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 5 cut(s) 53, 83, 103, 688, 838
BstNSI RCATGY 2 cut(s) 724, 944
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 43
BtsCI GGATG 3 cut(s) 151, 232, 843
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 640, 849
Cac8I GCNNGC 2 cut(s) 45, 722
Cfr13I GGNCC 2 cut(s) 927, 955
CseI GACGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 230
CviAII CATG 4 cut(s) 92, 553, 721, 941
CviQI GTAC 1 cut(s) 230
DdeI CTNAG 5 cut(s) 24, 34, 98, 359, 641
DpnI GATC 4 cut(s) 353, 587, 646, 935
DpnII GATC 4 cut(s) 351, 585, 644, 933
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EciI GGCGGA 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 20, 644
Eco47I GGWCC 2 cut(s) 927, 955
Eco53kI GAGCTC 1 cut(s) 97
Eco88I CYCGRG 2 cut(s) 140, 445
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 955
EcoT22I ATGCAT 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 4 cut(s) 95, 556, 724, 944
FaiI YATR 8 cut(s) 54, 93, 341, 521, 554, 576, 722, 942
FaqI GGGAC 2 cut(s) 929, 941
FatI CATG 4 cut(s) 91, 552, 720, 940
FauI CCCGC 1 cut(s) 869
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 2 cut(s) 57, 830
FokI GGATG 3 cut(s) 158, 239, 850
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 2 cut(s) 57, 830
FspBI CTAG 1 cut(s) 44
GluI GCNGC 2 cut(s) 57, 830
GsuI CTGGAG 1 cut(s) 806
HgaI GACGC 1 cut(s) 268
Hin1II CATG 4 cut(s) 95, 556, 724, 944
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 6 cut(s) 169, 363, 529, 536, 662, 698
HphI GGTGA 4 cut(s) 412, 485, 769, 808
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 4 cut(s) 251, 362, 644, 731
Hpy188III TCNNGA 6 cut(s) 100, 445, 533, 695, 758, 884
Hpy8I GTNNAC 1 cut(s) 706
HpyAV CCTTC 2 cut(s) 311, 620
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 7 cut(s) 149, 166, 213, 271, 569, 841, 903
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 83, 103, 688, 838
HpyF3I CTNAG 5 cut(s) 24, 34, 98, 359, 641
Hsp92II CATG 4 cut(s) 95, 556, 724, 944
KflI GGGWCCC 1 cut(s) 955
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 4 cut(s) 351, 585, 644, 933
LmnI GCTCC 3 cut(s) 261, 276, 787
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 5 cut(s) 136, 208, 639, 828, 890
MaeI CTAG 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 327, 765
MalI GATC 4 cut(s) 353, 587, 646, 935
MboI GATC 4 cut(s) 351, 585, 644, 933
MboII GAAGA 3 cut(s) 166, 488, 568
MfeI CAATTG 3 cut(s) 161, 506, 685
MhlI GDGCHC 3 cut(s) 88, 99, 141
MluCI AATT 7 cut(s) 161, 239, 506, 667, 685, 815, 887
MlyI GAGTC 2 cut(s) 357, 707
Mph1103I ATGCAT 1 cut(s) 843
MseI TTAA 4 cut(s) 296, 374, 440, 611
MspCI CTTAAG 1 cut(s) 295
MunI CAATTG 3 cut(s) 161, 506, 685
Mva1269I GAATGC 2 cut(s) 569, 812
MwoI GCNNNNNNNGC 5 cut(s) 53, 83, 103, 688, 838
NdeII GATC 4 cut(s) 351, 585, 644, 933
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 4 cut(s) 95, 556, 724, 944
NlaIV GGNNCC 3 cut(s) 85, 956, 957
NmeAIII GCCGAG 1 cut(s) 247
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 2 cut(s) 724, 944
PaeI GCATGC 1 cut(s) 724
PaeR7I CTCGAG 2 cut(s) 140, 445
PciI ACATGT 1 cut(s) 940
PctI GAATGC 2 cut(s) 569, 812
PfeI GAWTC 4 cut(s) 169, 529, 536, 662
PflMI CCANNNNNTGG 1 cut(s) 580
PkrI GCNGC 2 cut(s) 58, 831
Ple19I CGATCG 1 cut(s) 588
PleI GAGTC 2 cut(s) 357, 706
PpsI GAGTC 2 cut(s) 357, 706
PpuMI RGGWCCY 1 cut(s) 955
PscI ACATGT 1 cut(s) 940
PshBI ATTAAT 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 955
PspN4I GGNNCC 3 cut(s) 85, 956, 957
PspPI GGNCC 2 cut(s) 927, 955
PspPPI RGGWCCY 1 cut(s) 955
PspXI VCTCGAGB 1 cut(s) 140
PvuI CGATCG 1 cut(s) 588
RgaI GCGATCGC 1 cut(s) 588
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 296, 374, 440, 611
SatI GCNGC 2 cut(s) 57, 830
Sau3AI GATC 4 cut(s) 351, 585, 644, 933
Sau96I GGNCC 2 cut(s) 927, 955
SchI GAGTC 2 cut(s) 357, 707
SduI GDGCHC 3 cut(s) 88, 99, 141
SfaAI GCGATCGC 1 cut(s) 588
SfaNI GCATC 5 cut(s) 136, 208, 639, 828, 890
Sfr274I CTCGAG 2 cut(s) 140, 445
SgfI GCGATCGC 1 cut(s) 588
SinI GGWCC 2 cut(s) 927, 955
SlaI CTCGAG 2 cut(s) 140, 445
SmlI CTYRAG 6 cut(s) 140, 295, 320, 445, 605, 856
SmoI CTYRAG 6 cut(s) 140, 295, 320, 445, 605, 856
SphI GCATGC 1 cut(s) 724
Sse9I AATT 7 cut(s) 161, 239, 506, 667, 685, 815, 887
SsiI CCGC 3 cut(s) 259, 830, 876
SspMI CTAG 1 cut(s) 44
SstI GAGCTC 1 cut(s) 99
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 141, 446
TasI AATT 7 cut(s) 161, 239, 506, 667, 685, 815, 887
TatI WGTACW 1 cut(s) 229
TauI GCSGC 1 cut(s) 832
TfiI GAWTC 4 cut(s) 169, 529, 536, 662
Tru1I TTAA 4 cut(s) 296, 374, 440, 611
Tru9I TTAA 4 cut(s) 296, 374, 440, 611
TscAI CASTG 2 cut(s) 640, 856
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 3 cut(s) 289, 491, 691
TspRI CASTG 2 cut(s) 640, 856
Van91I CCANNNNNTGG 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 295
VpaK11BI GGWCC 2 cut(s) 927, 955
VspI ATTAAT 1 cut(s) 440
XapI RAATTY 2 cut(s) 239, 667
XceI RCATGY 2 cut(s) 724, 944
XhoI CTCGAG 2 cut(s) 140, 445
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.