Rh2AG269300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000159
Physical Location & Seq
Reverse (-)
31919220 .. 31920573
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG269300.1

Sequence Viewer

Length: 648 bp
ATGGTGATTGATGGTTGTGATAGTTTGGAATCCTTGCCACCAAATATGACCGCTTTAGATACTTTGGCTATCGATAATTGCGAGAAGCTTCAATTGATGATGAGATCAGGGGAAGGTCCTCAAGGTCTTCGATCATTGATTATCGGGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTTGAAGATTCTGCACACACTCTACAGAGTATATGGCTTCGTGAATGTCATAATCTCACGGCACTTCCGGAGTTGATAAACTTCACATTCCTCGAGCAAATACACATTGTAGAATGCTCCAAATTGTCGGCTTTGCCGCAAGGGTTGCATTATCTTACTCATTTGAGAGGATTGAACATTGAAGACTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGCTTGGTCAAAGATGGCACGGCAGGCAAAGATTACACTCGACTCTGATGATGAAGAAGACGAAGATGAAGGTCAGAGGAAGAAGAGGGTTGCTAGCTACAAGGTGTACACTGTGGAGGGGAAGCTGAAAGGGACACTCAGGAAGAGCTTCAGGTGGCTCCAGGAGACCTGTGATAGAGTGGTCTATAGCCGGCTTTCCAGCAAAAGCATGAGTGCCTCCATTGGAGGTGCTTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

215

Amino Acids

24.11

Weight (kDa)

5.99

Isoelectric Point (pI)

69.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 40 - 95 3.8e-06 R13L1/DRL21 LRRs
DUF3511 PF12023 157 - 198 3.8e-20 Domain of unknown function (DUF3511)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 2 cut(s) 51, 314
AcsI RAATTY 1 cut(s) 148
AcuI CTGAAG 1 cut(s) 541
AfaI GTAC 1 cut(s) 515
AgsI TTSAA 6 cut(s) 92, 153, 182, 352, 359, 642
AjnI CCWGG 1 cut(s) 567
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 5 cut(s) 88, 384, 504, 532, 555
AluI AGCT 5 cut(s) 88, 384, 504, 532, 555
Alw26I GTCTC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 269
Aor13HI TCCGGA 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 16, 416
AsuNHI GCTAGC 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 116
BbsI GAAGAC 3 cut(s) 119, 366, 471
BbvI GCAGC 1 cut(s) 371
BccI CCATC 2 cut(s) 5, 415
BceAI ACGGC 2 cut(s) 252, 443
BciT130I CCWGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 2 cut(s) 200, 592
BglI GCCNNNNNGGC 1 cut(s) 175
BisI GCNGC 2 cut(s) 314, 385
BlsI GCNGC 2 cut(s) 315, 386
Bme1390I CCNGG 1 cut(s) 569
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 1 cut(s) 569
BmtI GCTAGC 1 cut(s) 504
BpiI GAAGAC 3 cut(s) 119, 366, 471
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 597
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 1 cut(s) 569
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 173
BseMII CTCAG 1 cut(s) 559
BseXI GCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 245, 598
BslFI GGGAC 1 cut(s) 553
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 1 cut(s) 553
BsmI GAATGC 1 cut(s) 296
Bso31I GGTCTC 1 cut(s) 566
BsoBI CYCGRG 1 cut(s) 269
Bsp13I TCCGGA 1 cut(s) 244
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 2 cut(s) 104, 131
BspACI CCGC 2 cut(s) 51, 314
BspCNI CTCAG 1 cut(s) 558
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 566
BspOI GCTAGC 1 cut(s) 504
BspQI GCTCTTC 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 566
BsrFI RCCGGY 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 2 cut(s) 104, 131
BssT1I CCWWGG 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 365, 520
Bst6I CTCTTC 2 cut(s) 485, 545
BstAPI GCANNNNNTGC 2 cut(s) 322, 384
BstAUI TGTACA 1 cut(s) 513
BstC8I GCNNGC 3 cut(s) 432, 502, 599
BstDEI CTNAG 1 cut(s) 545
BstKTI GATC 2 cut(s) 107, 134
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 2 cut(s) 104, 131
BstMWI GCNNNNNNNGC 4 cut(s) 175, 322, 384, 431
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 1 cut(s) 567
BstSFI CTRYAG 2 cut(s) 200, 592
BstV1I GCAGC 1 cut(s) 371
BstV2I GAAGAC 3 cut(s) 119, 366, 471
Bsu15I ATCGAT 1 cut(s) 72
BsuTUI ATCGAT 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 3 cut(s) 432, 502, 599
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 1 cut(s) 514
CviAII CATG 1 cut(s) 616
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 1 cut(s) 545
DpnI GATC 2 cut(s) 106, 133
DpnII GATC 2 cut(s) 104, 131
Eam1104I CTCTTC 2 cut(s) 485, 545
EarI CTCTTC 2 cut(s) 485, 545
Eco130I CCWWGG 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 541
Eco88I CYCGRG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 1 cut(s) 619
FaiI YATR 6 cut(s) 47, 209, 211, 228, 594, 617
FaqI GGGAC 1 cut(s) 553
FatI CATG 1 cut(s) 615
Fnu4HI GCNGC 2 cut(s) 314, 385
Fsp4HI GCNGC 2 cut(s) 314, 385
FspBI CTAG 1 cut(s) 501
GluI GCNGC 2 cut(s) 314, 385
GsuI CTGGAG 1 cut(s) 551
HapII CCGG 2 cut(s) 245, 598
Hin1II CATG 1 cut(s) 619
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 29, 185, 449
HpaII CCGG 2 cut(s) 245, 598
HphI GGTGA 2 cut(s) 16, 416
Hpy166II GTNNAC 2 cut(s) 514, 516
Hpy188I TCNGA 2 cut(s) 454, 483
Hpy188III TCNNGA 5 cut(s) 145, 218, 245, 368, 547
Hpy8I GTNNAC 2 cut(s) 514, 516
HpyAV CCTTC 2 cut(s) 107, 470
HpyCH4III ACNGT 2 cut(s) 365, 520
HpyCH4V TGCA 3 cut(s) 191, 325, 387
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 322, 384, 431
HpyF3I CTNAG 1 cut(s) 545
Hsp92II CATG 1 cut(s) 619
Kpn2I TCCGGA 1 cut(s) 244
KroI GCCGGC 1 cut(s) 597
KroNI GCCGGC 1 cut(s) 599
Kzo9I GATC 2 cut(s) 104, 131
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 2 cut(s) 299, 570
Lsp1109I GCAGC 1 cut(s) 371
MaeI CTAG 1 cut(s) 501
MalI GATC 2 cut(s) 106, 133
MboI GATC 2 cut(s) 104, 131
MboII GAAGA 9 cut(s) 119, 194, 371, 473, 476, 482, 499, 502, 562
MfeI CAATTG 1 cut(s) 92
MluCI AATT 6 cut(s) 76, 92, 148, 299, 371, 395
MlyI GAGTC 1 cut(s) 443
MroI TCCGGA 1 cut(s) 244
MroNI GCCGGC 1 cut(s) 597
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 1 cut(s) 398
MspI CCGG 2 cut(s) 245, 598
MspR9I CCNGG 1 cut(s) 569
MunI CAATTG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 4 cut(s) 175, 322, 384, 431
NaeI GCCGGC 1 cut(s) 599
NdeII GATC 2 cut(s) 104, 131
NgoMIV GCCGGC 1 cut(s) 597
NheI GCTAGC 1 cut(s) 500
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 1 cut(s) 566
PaeR7I CTCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 1 cut(s) 296
PdiI GCCGGC 1 cut(s) 599
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 29, 185
PfoI TCCNGGA 1 cut(s) 567
PkrI GCNGC 2 cut(s) 315, 386
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PpuMI RGGWCCY 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 1 cut(s) 116
PspPPI RGGWCCY 1 cut(s) 116
PspXI VCTCGAGB 1 cut(s) 269
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 2 cut(s) 314, 385
Sau3AI GATC 2 cut(s) 104, 131
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 569
SfcI CTRYAG 2 cut(s) 200, 592
Sfr274I CTCGAG 1 cut(s) 269
SinI GGWCC 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 120, 269
SmoI CTYRAG 2 cut(s) 120, 269
Sse9I AATT 6 cut(s) 76, 92, 148, 299, 371, 395
SsiI CCGC 2 cut(s) 51, 314
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 567
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 365, 520
TaqI TCGA 4 cut(s) 72, 130, 270, 447
TasI AATT 6 cut(s) 76, 92, 148, 299, 371, 395
TatI WGTACW 1 cut(s) 513
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 2 cut(s) 29, 185
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 1 cut(s) 523
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 2 cut(s) 474, 489
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.