RchiOBHm_Chr2g0116691

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000159
Physical Location & Seq
Forward (+)
28979611 .. 28981375
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48971

Sequence Viewer

Length: 1647 bp
ATGCATTCTTTAGAAGGTCTTCCCCACAAAGACTGCATGTCATTGTTTATCAAAAGGGCATTTAAGAAAGGAGAGGAGCAGCACTATCAACATTTGATAGAAATTGGAGAAGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCGGTAACTACTGTAGGGAGCATGCTCTATTTGAATAGAGAGCGACACCATTGGTTGCGTGTCATAGATGATGACATGTGGAGTATAGGGAATGACAATATTTTACCTGCACTAAAATTGAGCTATGATGCATTGCCACAACACTTAAAACCGTGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTATGTATTCAATAGTCAACTATTGGTTTCATTGTGGATGGCACAAGGCTACCTCAAGACATCTATGAAAAATGAAGATTTTGAACATATAGGTGAAGAGTATATAAGGCAGTTTTGCTCTAGATCTTTGTTTCAAGTCGAGATAGATGTCAAAACAGCCATAGCTTTTAAAATACATGATCTAGTTCATGATCTAGTAATTTCAGTGGCACAAATAGAGTACTCCACAGTCAATTTTCGTCCCTCTAGTGCTTTTGAAATGGTTCAACATGTTTCAATATCTGAAAAGGACTTGCTTGGAGAAGAGGCACAAGTTCCCGAATTCATGCTAAAGTCAAAGAAATTGCGAACCATTCTAGTTCCTAAAGATGGTCAAATGAGTCAACGTTTTGTGAACACATGCATCTCGAGATTCAAATATATGCGGGTGCTAGATCTCAGTTGGTCGCCTCAGGAGGAGCTACCAAGTTCCGTTGGTAGTTTATTTCATTTGAGATTTCTCAACTTGTCTGAAAATTGGAAGATAAAAAGGCTTCCCAATTCCATCAGTGAGCTGCTGAATTTGGAGAGCTTGGCCCTAACCGGTTGTGACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTCCGATACTTGGGGATAACTACACAACAGAAGTATTTGCCAAAAGGAATTAGACGCCTCACCTCACTTCAATCTTTATGTTTTGATGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGCACTTTGTCGATTAGCAGGTGTAATAATTTGGAATCGCTGCCACCAAATATGAAACACATGACCGCTTTACATAGTTTGGGTGTCGGTGGTGGTTGCGAGAAGCTTCAGATGATGAGAGCAGGGGAAGGTCCTCAAGGTCTTCGATCATTGATTATCAGGAATTCAAGTTCGGAGGCTTTGCCCCCTTGGCTTGAAGATTCTGCAGACACTCTACAGAGCATATGGCTTGGTGAATGTCATAATCTCACGGCACTTCCGGAGTTGATAAAGTTCACATTGCTCGAGCAACTACGCATTGAAGAATGCTCCAAATTGACGGCTTTGCCGCAAGAGTTGCATTGCCTTACTGAGTTGAGAGAATTGAAGATTATTGGTTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGGTGAAGATTACACTCGACAATCACTTGGCCGACGGTAGGATCGCGTACCTCACAATTCCTCGAGTAAGGACGCAAGGACGAATCAGAATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

548

Amino Acids

62.53

Weight (kDa)

7.55

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 106 - 176 1.1e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 244 - 440 3e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 412 - 471 9.8e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1113
Acc36I ACCTGC 2 cut(s) 259, 1113
AccII CGCG 1 cut(s) 1595
AccIII TCCGGA 1 cut(s) 1363
AciI CCGC 4 cut(s) 146, 754, 1170, 1433
AclI AACGTT 1 cut(s) 715
AclWI GGATC 2 cut(s) 1078, 1598
AcoI YGGCCR 1 cut(s) 1578
AcsI RAATTY 4 cut(s) 650, 889, 1267, 1638
AcuI CTGAAG 1 cut(s) 1196
AcyI GRCGYC 1 cut(s) 1018
AfaI GTAC 2 cut(s) 551, 1598
AfiI CCNNNNNNNGG 3 cut(s) 698, 973, 1587
AflIII ACRYGT 2 cut(s) 219, 598
AgeI ACCGGT 1 cut(s) 911
AhdI GACNNNNNGTC 1 cut(s) 37
AjiI CACGTC 1 cut(s) 1547
AloI GAACNNNNNNTCC 2 cut(s) 1258, 1290
AluBI AGCT 7 cut(s) 267, 494, 790, 883, 900, 1210, 1503
AluI AGCT 7 cut(s) 267, 494, 790, 883, 900, 1210, 1503
AlwI GGATC 2 cut(s) 1078, 1598
Ama87I CYCGRG 3 cut(s) 736, 1388, 1611
Aor13HI TCCGGA 1 cut(s) 1363
AoxI GGCC 2 cut(s) 903, 1578
ApeKI GCWGC 4 cut(s) 79, 883, 1144, 1503
ApoI RAATTY 4 cut(s) 650, 889, 1267, 1638
AsiGI ACCGGT 1 cut(s) 911
Asp700I GAANNNNTTC 2 cut(s) 18, 591
AspS9I GGNCC 2 cut(s) 904, 1235
AsuHPI GGTGA 5 cut(s) 434, 1015, 1349, 1535, 1564
AvaI CYCGRG 3 cut(s) 736, 1388, 1611
AvaII GGWCC 1 cut(s) 1235
AxyI CCTNAGG 1 cut(s) 780
BbsI GAAGAC 2 cut(s) 11, 1238
BbvI GCAGC 4 cut(s) 91, 870, 1131, 1490
BccI CCATC 5 cut(s) 361, 692, 881, 1043, 1534
BceAI ACGGC 2 cut(s) 1371, 1440
BclI TGATCA 1 cut(s) 957
BfaI CTAG 6 cut(s) 450, 512, 524, 576, 686, 761
BfmI CTRYAG 3 cut(s) 156, 1308, 1319
BfuAI ACCTGC 2 cut(s) 259, 1113
BglI GCCNNNNNGGC 1 cut(s) 1294
BglII AGATCT 2 cut(s) 452, 763
BisI GCNGC 5 cut(s) 80, 884, 1145, 1433, 1504
BlsI GCNGC 5 cut(s) 81, 885, 1146, 1434, 1505
BmcAI AGTACT 1 cut(s) 551
Bme18I GGWCC 1 cut(s) 1235
BmeRI GACNNNNNGTC 1 cut(s) 37
BmeT110I CYCGRG 3 cut(s) 736, 1388, 1611
BmgBI CACGTC 1 cut(s) 1547
BmgT120I GGNCC 2 cut(s) 904, 1235
BmiI GGNNCC 1 cut(s) 953
BmsI GCATC 2 cut(s) 262, 741
BpiI GAAGAC 2 cut(s) 11, 1238
BpuEI CTTGAG 3 cut(s) 368, 947, 1224
BsaHI GRCGYC 1 cut(s) 1018
BsaJI CCNNGG 1 cut(s) 1292
BsaWI WCCGGW 2 cut(s) 911, 1363
Bsc4I CCNNNNNNNGG 3 cut(s) 698, 973, 1587
Bse118I RCCGGY 1 cut(s) 911
Bse21I CCTNAGG 1 cut(s) 780
Bse3DI GCAATG 3 cut(s) 275, 1382, 1444
BseAI TCCGGA 1 cut(s) 1363
BseDI CCNNGG 1 cut(s) 1292
BseGI GGATG 2 cut(s) 372, 1058
BseLI CCNNNNNNNGG 3 cut(s) 698, 973, 1587
BseMI GCAATG 3 cut(s) 275, 1382, 1444
BseMII CTCAG 3 cut(s) 781, 794, 1446
BseRI GAGGAG 5 cut(s) 89, 147, 800, 944, 1541
BseXI GCAGC 4 cut(s) 91, 870, 1131, 1490
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 1595
BshFI GGCC 2 cut(s) 905, 1580
BshTI ACCGGT 1 cut(s) 911
BsiHKCI CYCGRG 3 cut(s) 736, 1388, 1611
BsiSI CCGG 2 cut(s) 912, 1364
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 3 cut(s) 698, 973, 1587
BsmFI GGGAC 1 cut(s) 555
BsmI GAATGC 2 cut(s) 4, 1415
BsnI GGCC 2 cut(s) 905, 1580
BsoBI CYCGRG 3 cut(s) 736, 1388, 1611
Bsp13I TCCGGA 1 cut(s) 1363
Bsp143I GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
BspACI CCGC 4 cut(s) 146, 754, 1170, 1433
BspANI GGCC 2 cut(s) 905, 1580
BspCNI CTCAG 3 cut(s) 780, 793, 1447
BspEI TCCGGA 1 cut(s) 1363
BspFNI CGCG 1 cut(s) 1595
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 953
BspMAI CTGCAG 1 cut(s) 1312
BspMI ACCTGC 2 cut(s) 259, 1113
BspPI GGATC 2 cut(s) 1078, 1598
BsrDI GCAATG 3 cut(s) 275, 1382, 1444
BsrFI RCCGGY 1 cut(s) 911
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 1 cut(s) 1292
BssMI GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
BssNI GRCGYC 1 cut(s) 1018
BssT1I CCWWGG 1 cut(s) 1292
Bst4CI ACNGT 4 cut(s) 157, 297, 559, 1586
Bst6I CTCTTC 3 cut(s) 420, 627, 1074
BstACI GRCGYC 1 cut(s) 1018
BstAPI GCANNNNNTGC 2 cut(s) 1441, 1503
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 3 cut(s) 767, 780, 1455
BstF5I GGATG 2 cut(s) 372, 1058
BstFNI CGCG 1 cut(s) 1595
BstKTI GATC 8 cut(s) 455, 511, 523, 766, 960, 1086, 1253, 1593
BstMBI GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
BstMWI GCNNNNNNNGC 3 cut(s) 1294, 1441, 1503
BstNSI RCATGY 5 cut(s) 40, 169, 223, 602, 732
BstSFI CTRYAG 3 cut(s) 156, 1308, 1319
BstUI CGCG 1 cut(s) 1595
BstV1I GCAGC 4 cut(s) 91, 870, 1131, 1490
BstV2I GAAGAC 2 cut(s) 11, 1238
BstX2I RGATCY 3 cut(s) 452, 763, 1083
BstYI RGATCY 3 cut(s) 452, 763, 1083
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 2 cut(s) 905, 1580
BtrI CACGTC 1 cut(s) 1547
BtsCI GGATG 2 cut(s) 372, 1058
BtsIMutI CAGTG 2 cut(s) 540, 883
BveI ACCTGC 2 cut(s) 259, 1113
Cac8I GCNNGC 1 cut(s) 167
CciI TCATGA 1 cut(s) 517
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 2 cut(s) 904, 1235
CseI GACGC 3 cut(s) 929, 1026, 1630
Csp6I GTAC 2 cut(s) 550, 1597
CspAI ACCGGT 1 cut(s) 911
CviAII CATG 9 cut(s) 37, 166, 220, 506, 518, 599, 655, 729, 1165
CviQI GTAC 2 cut(s) 550, 1597
DdeI CTNAG 3 cut(s) 767, 780, 1455
DpnI GATC 8 cut(s) 454, 510, 522, 765, 959, 1085, 1252, 1592
DpnII GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
DraI TTTAAA 1 cut(s) 499
DriI GACNNNNNGTC 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 1578
Eam1104I CTCTTC 3 cut(s) 420, 627, 1074
Eam1105I GACNNNNNGTC 1 cut(s) 37
EarI CTCTTC 3 cut(s) 420, 627, 1074
Eco130I CCWWGG 1 cut(s) 1292
Eco47I GGWCC 1 cut(s) 1235
Eco57I CTGAAG 1 cut(s) 1196
Eco81I CCTNAGG 1 cut(s) 780
Eco88I CYCGRG 3 cut(s) 736, 1388, 1611
EcoO109I RGGNCCY 1 cut(s) 1235
EcoRI GAATTC 3 cut(s) 650, 1267, 1638
EcoT14I CCWWGG 1 cut(s) 1292
EcoT22I ATGCAT 3 cut(s) 6, 277, 734
ErhI CCWWGG 1 cut(s) 1292
FaeI CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
FaqI GGGAC 1 cut(s) 555
FatI CATG 9 cut(s) 36, 165, 219, 505, 517, 598, 654, 728, 1164
FauI CCCGC 1 cut(s) 747
FauNDI CATATG 1 cut(s) 1328
FbaI TGATCA 1 cut(s) 957
Fnu4HI GCNGC 5 cut(s) 80, 884, 1145, 1433, 1504
FokI GGATG 2 cut(s) 379, 1065
Fsp4HI GCNGC 5 cut(s) 80, 884, 1145, 1433, 1504
FspBI CTAG 6 cut(s) 450, 512, 524, 576, 686, 761
GluI GCNGC 5 cut(s) 80, 884, 1145, 1433, 1504
HaeIII GGCC 2 cut(s) 905, 1580
HapII CCGG 2 cut(s) 912, 1364
HgaI GACGC 3 cut(s) 929, 1026, 1630
Hin1I GRCGYC 1 cut(s) 1018
Hin1II CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
HincII GTYRAC 2 cut(s) 347, 713
HindII GTYRAC 2 cut(s) 347, 713
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 5 cut(s) 709, 741, 1139, 1304, 1632
HpaII CCGG 2 cut(s) 912, 1364
HphI GGTGA 5 cut(s) 434, 1015, 1349, 1535, 1564
Hpy166II GTNNAC 5 cut(s) 314, 347, 713, 724, 1380
Hpy188I TCNGA 6 cut(s) 613, 841, 968, 1215, 1279, 1637
Hpy8I GTNNAC 5 cut(s) 314, 347, 713, 724, 1380
Hpy99I CGWCG 1 cut(s) 1586
HpyAV CCTTC 3 cut(s) 8, 1112, 1226
HpyCH4III ACNGT 4 cut(s) 157, 297, 559, 1586
HpyCH4IV ACGT 2 cut(s) 715, 1546
HpyCH4V TGCA 9 cut(s) 4, 36, 254, 275, 305, 732, 1310, 1444, 1506
HpyF10VI GCNNNNNNNGC 3 cut(s) 1294, 1441, 1503
HpyF3I CTNAG 3 cut(s) 767, 780, 1455
HpySE526I ACGT 2 cut(s) 715, 1546
Hsp92I GRCGYC 1 cut(s) 1018
Hsp92II CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
Kpn2I TCCGGA 1 cut(s) 1363
Ksp22I TGATCA 1 cut(s) 957
Kzo9I GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
LmnI GCTCC 4 cut(s) 76, 162, 787, 1418
Lsp1109I GCAGC 4 cut(s) 91, 870, 1131, 1490
LweI GCATC 2 cut(s) 262, 741
MaeI CTAG 6 cut(s) 450, 512, 524, 576, 686, 761
MaeII ACGT 2 cut(s) 715, 1546
MaeIII GTNAC 2 cut(s) 148, 917
MalI GATC 8 cut(s) 454, 510, 522, 765, 959, 1085, 1252, 1592
MboI GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
MflI RGATCY 3 cut(s) 452, 763, 1083
MlyI GAGTC 1 cut(s) 718
Mph1103I ATGCAT 3 cut(s) 6, 277, 734
MroI TCCGGA 1 cut(s) 1363
MroXI GAANNNNTTC 2 cut(s) 18, 591
MseI TTAA 5 cut(s) 63, 290, 498, 1065, 1517
MslI CAYNNNNRTG 1 cut(s) 420
MspI CCGG 2 cut(s) 912, 1364
Mva1269I GAATGC 2 cut(s) 4, 1415
MvnI CGCG 1 cut(s) 1595
MwoI GCNNNNNNNGC 3 cut(s) 1294, 1441, 1503
NdeI CATATG 1 cut(s) 1328
NdeII GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
NlaIII CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
NlaIV GGNNCC 1 cut(s) 953
NmuCI GTSAC 1 cut(s) 917
NsiI ATGCAT 3 cut(s) 6, 277, 734
NspI RCATGY 5 cut(s) 40, 169, 223, 602, 732
PaeI GCATGC 1 cut(s) 169
PaeR7I CTCGAG 3 cut(s) 736, 1388, 1611
PagI TCATGA 1 cut(s) 517
PaqCI CACCTGC 1 cut(s) 1113
PciI ACATGT 2 cut(s) 219, 598
PctI GAATGC 2 cut(s) 4, 1415
PdmI GAANNNNTTC 2 cut(s) 18, 591
PfeI GAWTC 4 cut(s) 741, 1139, 1304, 1632
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 5 cut(s) 81, 885, 1146, 1434, 1505
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PpuMI RGGWCCY 1 cut(s) 1235
PscI ACATGT 2 cut(s) 219, 598
Psp1406I AACGTT 1 cut(s) 715
Psp5II RGGWCCY 1 cut(s) 1235
PspN4I GGNNCC 1 cut(s) 953
PspPI GGNCC 2 cut(s) 904, 1235
PspPPI RGGWCCY 1 cut(s) 1235
PspXI VCTCGAGB 2 cut(s) 1388, 1611
PstI CTGCAG 1 cut(s) 1312
PsuI RGATCY 3 cut(s) 452, 763, 1083
RsaI GTAC 2 cut(s) 551, 1598
RsaNI GTAC 2 cut(s) 550, 1597
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 5 cut(s) 63, 290, 498, 1065, 1517
SatI GCNGC 5 cut(s) 80, 884, 1145, 1433, 1504
Sau3AI GATC 8 cut(s) 452, 508, 520, 763, 957, 1083, 1250, 1590
Sau96I GGNCC 2 cut(s) 904, 1235
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 1 cut(s) 718
SfaNI GCATC 2 cut(s) 262, 741
SfcI CTRYAG 3 cut(s) 156, 1308, 1319
Sfr274I CTCGAG 3 cut(s) 736, 1388, 1611
SinI GGWCC 1 cut(s) 1235
SlaI CTCGAG 3 cut(s) 736, 1388, 1611
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 6 cut(s) 383, 736, 926, 1239, 1388, 1611
SmoI CTYRAG 6 cut(s) 383, 736, 926, 1239, 1388, 1611
SphI GCATGC 1 cut(s) 169
SsiI CCGC 4 cut(s) 146, 754, 1170, 1433
SspI AATATT 1 cut(s) 244
SspMI CTAG 6 cut(s) 450, 512, 524, 576, 686, 761
StyI CCWWGG 1 cut(s) 1292
TaaI ACNGT 4 cut(s) 157, 297, 559, 1586
TaiI ACGT 2 cut(s) 718, 1549
TaqI TCGA 7 cut(s) 468, 737, 1115, 1249, 1389, 1566, 1612
TatI WGTACW 1 cut(s) 549
TauI GCSGC 1 cut(s) 1435
TfiI GAWTC 4 cut(s) 741, 1139, 1304, 1632
Tru1I TTAA 5 cut(s) 63, 290, 498, 1065, 1517
Tru9I TTAA 5 cut(s) 63, 290, 498, 1065, 1517
TscAI CASTG 2 cut(s) 540, 883
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 4 cut(s) 79, 883, 1144, 1503
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 7 cut(s) 348, 410, 417, 506, 643, 806, 1172
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 2 cut(s) 540, 883
VpaK11BI GGWCC 1 cut(s) 1235
XapI RAATTY 4 cut(s) 650, 889, 1267, 1638
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 5 cut(s) 40, 169, 223, 602, 732
XhoI CTCGAG 3 cut(s) 736, 1388, 1611
XmnI GAANNNNTTC 2 cut(s) 18, 591
XspI CTAG 6 cut(s) 450, 512, 524, 576, 686, 761
ZrmI AGTACT 1 cut(s) 551
Zsp2I ATGCAT 3 cut(s) 6, 277, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.