RchiOBHm_Chr2g0116321

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000159
Physical Location & Seq
Reverse (-)
28652259 .. 28655143
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48937

Sequence Viewer

Length: 2649 bp
ATGGAGTTCGTCTACGGCATTGCAGACAACGTCTTGGATAGGCTAGCTTCACATGCTTCCCAAGAGATCTCCTTGGCATGGGGTGCCCAACTTGAACTCACCAAGCTCAACAAGACCTTATCTACCATCAAACTAGTACTCGAAGATGCAGAGAAGAAGCAAGTGAGGAATTCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGATGTCTGTCATGACGTTGATGATGTCTTGGACGAACTGGACTTCCAAAAGTTGCGGCTGAAAGTGGAGGTCGACAACTGTGGGAAGATCAAAGGCAAGGTACGCCAATTTTTTTCCCGTTGGAATCCAATAGTGTTTAACTTCAAAATGGGACATAAAGTGAAAGAGATCAGAGAAAGACTAGTTGAAATTGATAATGAAAAGACACAATTTGGTCTCCTTAAGTTTGCTAAGGTAGCTGAGGATCCTCGTTTGCTCCAAGGGATGCACGATAATAAGAGAGAGACTGACTCTTTGGTGGAGGCTTCAGATGTTATTGGGAGAGATGATGATAAAAAACAGATTATTAGCCATCTCTTAAATAATACTGATATCTCTAGTGAGGAGAATGTTTCTGTTATTTCCATCCTTGGGTTAGGAGGGTTGGGGAAGACAACTCTAGCTAAATTGGTGTATAATGATAGCATAGTAGAGGAGAATTTTGAGATGAAAATGTGGATATGCGTCTCAGAAAATTTTGATATCGGAACATTAATTCGCAGCATTATCAATGCTGCAATTAAACAAAAATGTGAGGATGAAAGTTTGGACCTTATGAAGAGAAGATTGCAAGATACTTTGCGAGGTCGAAGATTTTTGTTAGTGTTGGATGATGTCTGGGATATGGAGCTAATTGGAATAACAATTGAAAAATGGAATGATCTAAAAGGCTTGTTAAATGTGGGAGCTAATGGAAGTAAAATCATCATAACAACACGACATGGATCAGTTGCTTCACTTGTGAATCCCGTATACATGCAGCCCTTAGAAGGTCTTCCCCACAAAGACTGCATCTCCTTGTTCATCAAAAGGGCATTTAAAAGAGGAGAGGAGCAGCGCTATCCACATTTGATAGAAATTGGTGAGGATATTGTGAAGAAGTGTCGAGGAGTACCTTTAGCAGTAGCTACTGTAGGGAGCATGCTCTATTTGAAAAGAGATGAACACCAATGGTCGAGTGTCAGAGATGATGACATGTGGAGCATAAGGAACGACAATATTTTACCTGCACTCAAATTGAGCTATGATGCACTGCCACAACACTTGAAACCGTGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTATGTGTTCAAGAGTCCAATGTTGGTCCCATTGTGGATAGCACAAGGATACCTCAAGACTTCTAAGAAAAATGAAGATTTTGAACAGATGGGTTTAGACTATATAAGGGAGTTTTGCTCTAGATCTTTGTTTCAAGTCGAGGGTGATATCAAAACAGCCATATTTTTTAAAATACATGATCTCATTCATGATTTAACAATTTCAGTGGCACAAGTAGAGTACTCCACAGTCAATTTTCGTCCCTCTAGTGATTTTGAAATGGTTCGACATGTGTCAATATCTAAAAAGGACTTGCTTGGGGAAGAGGCACAAGTTTCTGATTTCATGCTCACATCAAAGAAATTGCGAACCATTTTAGTTCCTGAAAAAGGTCAGATGAGTCAACGTTTTGTGAAGACATGCATTTTGAGATTCAAATATATGCGGGTGCTAGATCTCAGGAGGTCGCCTCTTGAGGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATCTGAGATTTCTCAGCTTGTTTTCTAATAGGAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGTACTTGAACCTTGAGGGTTGCAACGCACTTGAGGAAATACCCAAAGACATAGGGAACCTGATCAACCTACGAAGCTTGACGATAACAACACAGCAGACGTATTTGCCAAAAGGAATTTGGCACCTCACCTTACTTCAATATTTAGAATTTCATGGATGCGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGCAAATTGTGGATTAGCAGTTGTAATAATTTGGTATCATTGCCACCAAATATGAAACACGCGACTGCTTTAGATACTTTTGGTGTCAATGATTGCGTCAAGCTTCAGGTGATGATGAGATCAGGGGAAGGTCCTCAACGTCTTCGATCATTGCTTATCGCGAAGTCAAGTTTGGAGGCTTTGCCCCCTTGGCTCATTGAAGATTCTGCAGACACTCTACAGAGCATAGTACTTTCTGAATGTCATAATCTCACGGCACTTCCGCAGTTGATAAAGTTCAGATTCCTCGAGCAACTACGCATTGAAGAATGCTTCAAATTGTCGGCTTTGCCGCAGGAGTTGCATTGCCTTACTGAGTTGAGAGAACTGTATATTGAAGGATGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCACGTGAAGATTACACTCGACGCTGATAATGAAGAAGATGAAGCCGACAATCGCTTGGCCGATGGTAGGATCGCGTACCACACGCGACGACGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

882

Amino Acids

101.32

Weight (kDa)

7.52

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 93 3.8e-19 Rx N-terminal domain
NB-ARC PF00931 177 - 356 1.4e-31 NB-ARC domain
WHD_DRP PF23559 439 - 508 7.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 777 4.4e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 746 - 805 1.4e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1258
AccB1I GGYRCC 2 cut(s) 83, 2016
AccI GTMKAC 3 cut(s) 12, 276, 996
AccII CGCG 4 cut(s) 2165, 2264, 2624, 2635
AciI CCGC 4 cut(s) 259, 1753, 2366, 2435
AclI AACGTT 1 cut(s) 1714
AclWI GGATC 5 cut(s) 443, 456, 976, 2077, 2627
AcoI YGGCCR 1 cut(s) 2607
AcsI RAATTY 6 cut(s) 169, 682, 718, 1888, 2010, 2042
AcuI CTGAAG 2 cut(s) 495, 2192
AcvI CACGTG 1 cut(s) 2554
AcyI GRCGYC 1 cut(s) 2641
AfaI GTAC 7 cut(s) 138, 306, 1137, 1550, 1898, 2334, 2627
AfeI AGCGCT 1 cut(s) 1082
AfiI CCNNNNNNNGG 5 cut(s) 78, 615, 1013, 1697, 2616
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 2 cut(s) 1218, 1597
AhlI ACTAGT 2 cut(s) 133, 385
AjiI CACGTC 1 cut(s) 2549
AjuI GAANNNNNNNTTGG 2 cut(s) 2258, 2290
AloI GAACNNNNNNTCC 4 cut(s) 231, 263, 1319, 1351
Alw26I GTCTC 3 cut(s) 425, 482, 715
AlwI GGATC 5 cut(s) 443, 456, 976, 2077, 2627
Ama87I CYCGRG 1 cut(s) 2390
Aor51HI AGCGCT 1 cut(s) 1082
AoxI GGCC 1 cut(s) 2607
ApeKI GCWGC 6 cut(s) 744, 758, 1003, 1078, 1882, 2505
ApoI RAATTY 6 cut(s) 169, 682, 718, 1888, 2010, 2042
ArsI GACNNNNNNTTYG 4 cut(s) 481, 513, 1717, 1749
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 1017, 1590
AspLEI GCGC 1 cut(s) 1083
AspS9I GGNCC 3 cut(s) 793, 1354, 2234
AsuHPI GGTGA 6 cut(s) 91, 1118, 1484, 2014, 2224, 2537
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 2390
AvaII GGWCC 3 cut(s) 793, 1354, 2234
BaeGI GKGCMC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 2 cut(s) 83, 2016
BbrPI CACGTG 1 cut(s) 2554
BbsI GAAGAC 4 cut(s) 641, 1010, 1730, 2237
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 6 cut(s) 745, 756, 1015, 1090, 1869, 2492
BccI CCATC 7 cut(s) 134, 564, 617, 1411, 1880, 2536, 2606
BceAI ACGGC 3 cut(s) 31, 2373, 2628
BciVI GTATCC 1 cut(s) 1370
BclI TGATCA 1 cut(s) 1956
BcoDI GTCTC 3 cut(s) 425, 482, 715
BcuI ACTAGT 2 cut(s) 133, 385
BfaI CTAG 8 cut(s) 44, 134, 386, 582, 644, 1449, 1575, 1760
BfmI CTRYAG 3 cut(s) 1155, 2310, 2321
BfoI RGCGCY 1 cut(s) 1084
BfrI CTTAAG 1 cut(s) 425
BfuAI ACCTGC 1 cut(s) 1258
BfuI GTATCC 1 cut(s) 1370
BglI GCCNNNNNGGC 1 cut(s) 2293
BglII AGATCT 3 cut(s) 66, 1451, 1762
BisI GCNGC 8 cut(s) 260, 745, 759, 1004, 1079, 1883, 2435, 2506
BlsI GCNGC 8 cut(s) 261, 746, 760, 1005, 1080, 1884, 2436, 2507
BmcAI AGTACT 4 cut(s) 138, 1550, 1898, 2334
Bme18I GGWCC 3 cut(s) 793, 1354, 2234
BmeT110I CYCGRG 1 cut(s) 2390
BmgBI CACGTC 1 cut(s) 2549
BmgT120I GGNCC 3 cut(s) 793, 1354, 2234
BmiI GGNNCC 5 cut(s) 85, 450, 1356, 1952, 2018
BmsI GCATC 5 cut(s) 136, 459, 1044, 1261, 2042
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1600
BpiI GAAGAC 4 cut(s) 641, 1010, 1730, 2237
BplI GAGNNNNNCTC 6 cut(s) 479, 511, 1430, 1462, 1762, 1794
Bpu10I CCTNAGC 2 cut(s) 435, 444
BpuEI CTTGAG 4 cut(s) 1367, 1802, 1928, 1946
BsaAI YACGTR 1 cut(s) 2554
BsaHI GRCGYC 1 cut(s) 2641
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 4 cut(s) 72, 463, 613, 2291
BsaXI ACNNNNNCTCC 2 cut(s) 1022, 1052
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 615, 1013, 1697, 2616
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 4 cut(s) 18, 2141, 2252, 2446
BseDI CCNNGG 4 cut(s) 72, 463, 613, 2291
BseGI GGATG 6 cut(s) 474, 609, 787, 859, 2057, 2489
BseLI CCNNNNNNNGG 5 cut(s) 78, 615, 1013, 1697, 2616
BseMI GCAATG 4 cut(s) 18, 2141, 2252, 2446
BseMII CTCAG 6 cut(s) 435, 726, 1780, 1811, 1843, 2448
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 7 cut(s) 602, 692, 1083, 1088, 1146, 1799, 2543
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 6 cut(s) 745, 756, 1015, 1090, 1869, 2492
BsgI GTGCAG 1 cut(s) 1236
Bsh1236I CGCG 4 cut(s) 2165, 2264, 2624, 2635
BshFI GGCC 1 cut(s) 2609
BshNI GGYRCC 2 cut(s) 83, 2016
BsiHKCI CYCGRG 1 cut(s) 2390
BslFI GGGAC 3 cut(s) 369, 1340, 1554
BslI CCNNNNNNNGG 5 cut(s) 78, 615, 1013, 1697, 2616
BsmAI GTCTC 3 cut(s) 425, 482, 715
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 3 cut(s) 369, 1340, 1554
BsmI GAATGC 1 cut(s) 2417
BsnI GGCC 1 cut(s) 2609
Bso31I GGTCTC 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 2390
Bsp1286I GDGCHC 1 cut(s) 88
Bsp68I TCGCGA 1 cut(s) 2264
BspACI CCGC 4 cut(s) 259, 1753, 2366, 2435
BspANI GGCC 1 cut(s) 2609
BspCNI CTCAG 6 cut(s) 436, 725, 1779, 1812, 1842, 2449
BspFNI CGCG 4 cut(s) 2165, 2264, 2624, 2635
BspHI TCATGA 2 cut(s) 214, 1516
BspLI GGNNCC 5 cut(s) 85, 450, 1356, 1952, 2018
BspMAI CTGCAG 1 cut(s) 2314
BspMI ACCTGC 1 cut(s) 1258
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 5 cut(s) 443, 456, 976, 2077, 2627
BspT107I GGYRCC 2 cut(s) 83, 2016
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 4 cut(s) 18, 2141, 2252, 2446
BsrI ACTGG 1 cut(s) 246
BssECI CCNNGG 4 cut(s) 72, 463, 613, 2291
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 1 cut(s) 2641
BssT1I CCWWGG 4 cut(s) 72, 463, 613, 2291
Bst1107I GTATAC 1 cut(s) 997
Bst4CI ACNGT 5 cut(s) 284, 1156, 1296, 1558, 2472
Bst6I CTCTTC 3 cut(s) 797, 1626, 2073
BstACI GRCGYC 1 cut(s) 2641
BstAFI CTTAAG 1 cut(s) 425
BstAPI GCANNNNNTGC 3 cut(s) 83, 2443, 2505
BstBAI YACGTR 1 cut(s) 2554
BstC8I GCNNGC 2 cut(s) 45, 1166
BstDEI CTNAG 9 cut(s) 435, 444, 712, 1009, 1392, 1766, 1820, 1829, 2457
BstENI CCTNNNNNAGG 1 cut(s) 1695
BstF5I GGATG 6 cut(s) 474, 609, 787, 859, 2057, 2489
BstFNI CGCG 4 cut(s) 2165, 2264, 2624, 2635
BstH2I RGCGCY 1 cut(s) 1084
BstHHI GCGC 1 cut(s) 1083
BstMAI GTCTC 3 cut(s) 425, 482, 715
BstMWI GCNNNNNNNGC 7 cut(s) 53, 83, 306, 440, 2293, 2443, 2505
BstNSI RCATGY 6 cut(s) 56, 1003, 1168, 1222, 1601, 1731
BstPAI GACNNNNGTC 1 cut(s) 1600
BstSFI CTRYAG 3 cut(s) 1155, 2310, 2321
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 4 cut(s) 2165, 2264, 2624, 2635
BstV1I GCAGC 6 cut(s) 745, 756, 1015, 1090, 1869, 2492
BstV2I GAAGAC 4 cut(s) 641, 1010, 1730, 2237
BstX2I RGATCY 5 cut(s) 66, 448, 1451, 1762, 2082
BstYI RGATCY 5 cut(s) 66, 448, 1451, 1762, 2082
BstZ17I GTATAC 1 cut(s) 997
BsuI GTATCC 1 cut(s) 1370
BsuRI GGCC 1 cut(s) 2609
BtrI CACGTC 1 cut(s) 2549
BtsCI GGATG 6 cut(s) 474, 609, 787, 859, 2057, 2489
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 2 cut(s) 1274, 1539
BtuMI TCGCGA 1 cut(s) 2264
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 2 cut(s) 45, 1166
CciI TCATGA 2 cut(s) 214, 1516
CfoI GCGC 1 cut(s) 1083
Cfr13I GGNCC 3 cut(s) 793, 1354, 2234
CseI GACGC 4 cut(s) 697, 2044, 2188, 2579
Csp6I GTAC 7 cut(s) 137, 305, 1136, 1549, 1897, 2333, 2626
CspCI CAANNNNNGTGG 4 cut(s) 1269, 1304, 1515, 1550
CviQI GTAC 7 cut(s) 137, 305, 1136, 1549, 1897, 2333, 2626
DdeI CTNAG 9 cut(s) 435, 444, 712, 1009, 1392, 1766, 1820, 1829, 2457
DraI TTTAAA 2 cut(s) 1063, 1498
EaeI YGGCCR 1 cut(s) 2607
Eam1104I CTCTTC 3 cut(s) 797, 1626, 2073
EarI CTCTTC 3 cut(s) 797, 1626, 2073
Eco130I CCWWGG 4 cut(s) 72, 463, 613, 2291
Eco31I GGTCTC 1 cut(s) 425
Eco32I GATATC 3 cut(s) 577, 727, 1477
Eco47I GGWCC 3 cut(s) 793, 1354, 2234
Eco47III AGCGCT 1 cut(s) 1082
Eco57I CTGAAG 2 cut(s) 495, 2192
Eco72I CACGTG 1 cut(s) 2554
Eco88I CYCGRG 1 cut(s) 2390
EcoNI CCTNNNNNAGG 1 cut(s) 1695
EcoO109I RGGNCCY 1 cut(s) 2234
EcoRI GAATTC 1 cut(s) 169
EcoRV GATATC 3 cut(s) 577, 727, 1477
EcoT14I CCWWGG 4 cut(s) 72, 463, 613, 2291
EcoT22I ATGCAT 1 cut(s) 1733
ErhI CCWWGG 4 cut(s) 72, 463, 613, 2291
Esp3I CGTCTC 1 cut(s) 715
FaqI GGGAC 3 cut(s) 369, 1340, 1554
FauI CCCGC 1 cut(s) 1746
FbaI TGATCA 1 cut(s) 1956
FblI GTMKAC 3 cut(s) 12, 276, 996
Fnu4HI GCNGC 8 cut(s) 260, 745, 759, 1004, 1079, 1883, 2435, 2506
FokI GGATG 6 cut(s) 481, 596, 794, 866, 2064, 2496
Fsp4HI GCNGC 8 cut(s) 260, 745, 759, 1004, 1079, 1883, 2435, 2506
FspBI CTAG 8 cut(s) 44, 134, 386, 582, 644, 1449, 1575, 1760
GlaI GCGC 1 cut(s) 1082
GluI GCNGC 8 cut(s) 260, 745, 759, 1004, 1079, 1883, 2435, 2506
HaeII RGCGCY 1 cut(s) 1084
HaeIII GGCC 1 cut(s) 2609
HgaI GACGC 4 cut(s) 697, 2044, 2188, 2579
HhaI GCGC 1 cut(s) 1083
Hin1I GRCGYC 1 cut(s) 2641
Hin6I GCGC 1 cut(s) 1081
HinP1I GCGC 1 cut(s) 1081
HincII GTYRAC 2 cut(s) 277, 1712
HindII GTYRAC 2 cut(s) 277, 1712
HindIII AAGCTT 2 cut(s) 1969, 2204
HinfI GANTC 8 cut(s) 328, 494, 988, 1342, 1708, 1740, 2306, 2385
HphI GGTGA 6 cut(s) 91, 1118, 1484, 2014, 2224, 2537
Hpy166II GTNNAC 5 cut(s) 13, 277, 997, 1313, 1712
Hpy8I GTNNAC 5 cut(s) 13, 277, 997, 1313, 1712
Hpy99I CGWCG 3 cut(s) 2573, 2640, 2643
HpyAV CCTTC 4 cut(s) 1007, 2111, 2225, 2474
HpyCH4III ACNGT 5 cut(s) 284, 1156, 1296, 1558, 2472
HpyCH4IV ACGT 7 cut(s) 30, 219, 1714, 1994, 2242, 2548, 2553
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 83, 306, 440, 2293, 2443, 2505
HpyF3I CTNAG 9 cut(s) 435, 444, 712, 1009, 1392, 1766, 1820, 1829, 2457
HpySE526I ACGT 7 cut(s) 30, 219, 1714, 1994, 2242, 2548, 2553
Hsp92I GRCGYC 1 cut(s) 2641
HspAI GCGC 1 cut(s) 1081
Ksp22I TGATCA 1 cut(s) 1956
LmnI GCTCC 7 cut(s) 465, 871, 929, 1075, 1161, 1224, 1786
Lsp1109I GCAGC 6 cut(s) 745, 756, 1015, 1090, 1869, 2492
LweI GCATC 5 cut(s) 136, 459, 1044, 1261, 2042
MaeI CTAG 8 cut(s) 44, 134, 386, 582, 644, 1449, 1575, 1760
MaeII ACGT 7 cut(s) 30, 219, 1714, 1994, 2242, 2548, 2553
MaeIII GTNAC 1 cut(s) 2549
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 5 cut(s) 66, 448, 1451, 1762, 2082
MhlI GDGCHC 1 cut(s) 88
MlyI GAGTC 3 cut(s) 488, 1351, 1717
MmeI TCCRAC 2 cut(s) 305, 831
Mph1103I ATGCAT 1 cut(s) 1733
MroXI GAANNNNTTC 2 cut(s) 1017, 1590
MspCI CTTAAG 1 cut(s) 425
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 2417
MvnI CGCG 4 cut(s) 2165, 2264, 2624, 2635
MwoI GCNNNNNNNGC 7 cut(s) 53, 83, 306, 440, 2293, 2443, 2505
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 5 cut(s) 85, 450, 1356, 1952, 2018
NmuCI GTSAC 1 cut(s) 2549
NruI TCGCGA 1 cut(s) 2264
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 6 cut(s) 56, 1003, 1168, 1222, 1601, 1731
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 1 cut(s) 2390
PagI TCATGA 2 cut(s) 214, 1516
PciI ACATGT 2 cut(s) 1218, 1597
PctI GAATGC 1 cut(s) 2417
PdmI GAANNNNTTC 2 cut(s) 1017, 1590
PfeI GAWTC 5 cut(s) 328, 988, 1740, 2306, 2385
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 8 cut(s) 261, 746, 760, 1005, 1080, 1884, 2436, 2507
PleI GAGTC 3 cut(s) 488, 1350, 1716
PmaCI CACGTG 1 cut(s) 2554
PmlI CACGTG 1 cut(s) 2554
PpsI GAGTC 3 cut(s) 488, 1350, 1716
Ppu21I YACGTR 1 cut(s) 2554
PpuMI RGGWCCY 1 cut(s) 2234
PscI ACATGT 2 cut(s) 1218, 1597
PshAI GACNNNNGTC 1 cut(s) 1600
PshBI ATTAAT 1 cut(s) 737
Psp1406I AACGTT 1 cut(s) 1714
Psp5II RGGWCCY 1 cut(s) 2234
PspCI CACGTG 1 cut(s) 2554
PspN4I GGNNCC 5 cut(s) 85, 450, 1356, 1952, 2018
PspPI GGNCC 3 cut(s) 793, 1354, 2234
PspPPI RGGWCCY 1 cut(s) 2234
PspXI VCTCGAGB 1 cut(s) 2390
PstI CTGCAG 1 cut(s) 2314
PsuI RGATCY 5 cut(s) 66, 448, 1451, 1762, 2082
PsyI GACNNNGTC 1 cut(s) 29
RruI TCGCGA 1 cut(s) 2264
RsaI GTAC 7 cut(s) 138, 306, 1137, 1550, 1898, 2334, 2627
RsaNI GTAC 7 cut(s) 137, 305, 1136, 1549, 1897, 2333, 2626
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 8 cut(s) 260, 745, 759, 1004, 1079, 1883, 2435, 2506
Sau96I GGNCC 3 cut(s) 793, 1354, 2234
ScaI AGTACT 4 cut(s) 138, 1550, 1898, 2334
SchI GAGTC 3 cut(s) 488, 1351, 1717
SduI GDGCHC 1 cut(s) 88
SfaNI GCATC 5 cut(s) 136, 459, 1044, 1261, 2042
SfcI CTRYAG 3 cut(s) 1155, 2310, 2321
Sfr274I CTCGAG 1 cut(s) 2390
SinI GGWCC 3 cut(s) 793, 1354, 2234
SlaI CTCGAG 1 cut(s) 2390
SmlI CTYRAG 6 cut(s) 425, 1382, 1781, 1907, 1925, 2390
SmoI CTYRAG 6 cut(s) 425, 1382, 1781, 1907, 1925, 2390
SpeI ACTAGT 2 cut(s) 133, 385
SphI GCATGC 1 cut(s) 1168
SsiI CCGC 4 cut(s) 259, 1753, 2366, 2435
SspI AATATT 2 cut(s) 1243, 2036
SspMI CTAG 8 cut(s) 44, 134, 386, 582, 644, 1449, 1575, 1760
StyI CCWWGG 4 cut(s) 72, 463, 613, 2291
TaaI ACNGT 5 cut(s) 284, 1156, 1296, 1558, 2472
TaiI ACGT 7 cut(s) 33, 222, 1717, 1997, 2245, 2551, 2556
TatI WGTACW 4 cut(s) 136, 1548, 1896, 2332
TauI GCSGC 2 cut(s) 262, 2437
TfiI GAWTC 5 cut(s) 328, 988, 1740, 2306, 2385
TscAI CASTG 2 cut(s) 1281, 1539
TseFI GTSAC 1 cut(s) 2549
TseI GCWGC 6 cut(s) 744, 758, 1003, 1078, 1882, 2505
Tsp45I GTSAC 1 cut(s) 2549
TspRI CASTG 2 cut(s) 1281, 1539
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 3 cut(s) 793, 1354, 2234
VspI ATTAAT 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 1695
XapI RAATTY 6 cut(s) 169, 682, 718, 1888, 2010, 2042
XbaI TCTAGA 1 cut(s) 1448
XceI RCATGY 6 cut(s) 56, 1003, 1168, 1222, 1601, 1731
XcmI CCANNNNNNNNNTGG 1 cut(s) 2010
XhoI CTCGAG 1 cut(s) 2390
XmiI GTMKAC 3 cut(s) 12, 276, 996
XmnI GAANNNNTTC 2 cut(s) 1017, 1590
XspI CTAG 8 cut(s) 44, 134, 386, 582, 644, 1449, 1575, 1760
ZrmI AGTACT 4 cut(s) 138, 1550, 1898, 2334
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.