Rh2AG261000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000159
Physical Location & Seq
Reverse (-)
30058151 .. 30063261
5111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG261000.1

Sequence Viewer

Length: 2643 bp
ATGGAGTTCGTCTACGGCATTGCAGACAACGTCTTGGATAGGCTGGCTTCACATGCTTCCCAAGAGATCTCCTTGGCATGGGGTGCCCAACTTGAACTCACCAAGCTCAACAAGACCTTATCTACCATCAAACTGGTACTCGAAGATGCAGAGAAGAAGCAAGTGAGGAATTCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGACGTTTGTCATGACGTTGATGATGTCTTGGACGAACTGGACTTCCAAAAGTTGCGGCTGAAAGTGGAGGTCGACAACTGTGGGAAGATCAAAGGAAAGGTACGCCAATTTTTTTCCCGTTGGAATCCAATAGTGTTTAACTTCAAAATGGGACATAAAGTGAAAGAGATCAGAGAAAGACTAGTTGAAATTGATAATGAAAAGAGACAATTTGGTCTCCTTAAGTTTGCTAAGGTAGCTGAGGATCCTCGTGTGCTCCAAGGGATGCACGATAATAAGAGAGAGACTGACTCTTTGGTGGAGGCTTCAGATGTTATTGGGAGAGATGATGATAAAAAACAGATTATTAGTCATCTCTTAAATAATACTGATATCTCTAGTGAGGAGAATGTTTCTGTTATTTCCATCCTTGGGTTAGGAGGGTTGGGGAAGACAACTCTAGCTAAATTAGTGTATAACATGGTGGAGGAGAATTTTGAGATGAAAATGTGGATATGTGTCTCAGAAAACTTTGATATCGGAACATTAATTCGCAGCATTATCAATGCTGCAATTAAACAAAAATGTGAGGATGAAAGTTTGGACCTTATGAAGAGAAGATTGCAAGATACTTTGCGAGGTAGAAAGTTTTTGTTAGTGTTGGATGATGTCTGGGATATGGAGCTGATTGGAATAACGAATGAAAAATGGAATGATCTAAAAGGCTTGCTAAATGTGGGAGCTAGTGGAAGTACAATCATCATAACAACACGAAATAGATCAGTTGCTTTACTTGTGAGTCCCTTATACATGCATTCTTTGGAAGGTCTTCTCCACAAATACTGCATGTCATTGTTTATCAAAAGGGCATTTAAGAAAGGAGAGGAGCAACGCTATCCACATTTGATAGAAATTGGAGAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAGTAGCTACTGTAGGGAGCATGCTCCATTTGAAAAGAGATGAACACCAATGGTTGCGTGTTAGAGATGATGACATGTGGAGCATAAGGAATGATAATATTCTACCTGCGCTCAAATTGAGCTATGATGCATTGCCACAACACTTGAAACCGTGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTATGAATTCAAAAGTGCAATGTTGGTTCCATTGTGGACAGCACAAGGATACTTCAAGACATCTAAGAAAAATGAAGATTTTGAATGGATGGGTTTAGACTATATAAGGGAGTTTTGCTCTAGATCTTTGTTTCAAGTTGAGTGGGATCTCAAAACAGTCATAAGTTTTAAAATACATGATCTAGTTCATGATTTAGCAATTTCAGTGGCACAAGTAGAGTATTCCACAGTCAATTTCCGTCCCTCTAGTGCTTTGGAAATGGTTCGACATGTTTCAATATCCAAAAAAGACTTGCTTGGAGAAGAGGCACAAGTTCCCGATTTCATGCTCAAGTCAAAGAAATTGCAAACCATTCTAGTTTCTAAAGATGGTCAGATGAATCAACGTTTTGTGAAGACATGCATCTCCAGATTCAAATATATGCGGGTGCTAGATCTCAGTGGGTCGCCTCTTGAGGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATTTGAGATTCCTCGACTTTTCTGGTATGTGGAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGACCTTGGACCTTTGGGGTTGTGACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTCCGAGGCTTGAGGATAACTACACTACAGACGTATTTGCCAAAAGGAATTAGACGCCTCACCTTACTTCGATCTTTATATTTTAGTGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGTAAATTGCAGATTAGCAGGTGTAATAAGTTGGAATGCTTGCCACCAAATATGAAACACATGACCGCTTTGGATACTTTGGGTGTCTATGGTTGCGAGAAGCTTCAGGTGATGATGAGATCAGGGGAAGGTCCTCAACGTCTTCGATCATTGCTTATCGAGAAATCAAGTTTGGAGGCTTTGCCCCCTTGGCTTGTTGAAGATTCTGCAGACACTCTGCAGAGCATAGCACTTGGTGGATGTCATAATCTCACGGCACTTCCGGAGTCGATAAAGTTCAGATTCCTCGAGCAACTATACATTGGCGAATGCTCCAAATTGTCGGCTTTGCCGCAAGGGTTGCATTGCCTTACTGAATTGAGAGAACTGAACATTGAAGAGTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAACATGGCACGTCAGGTGAAGATTACACTCGACGCTGATGATGAAGAAGATGAAGCCGACAATCGCTTGGCCGACGGTAGGATAGCGTACTACACGCGACGACGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

880

Amino Acids

100.87

Weight (kDa)

7.74

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 93 2.3e-19 Rx N-terminal domain
NB-ARC PF00931 177 - 354 5.2e-29 NB-ARC domain
AAA_22 PF13401 200 - 314 1.9e-06 AAA domain
WHD_DRP PF23559 437 - 507 2.1e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 775 1.1e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 744 - 803 6.1e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2106
AasI GACNNNNNNGTC 1 cut(s) 417
Acc36I ACCTGC 2 cut(s) 1252, 2106
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 12, 276
AccII CGCG 1 cut(s) 2629
AccIII TCCGGA 1 cut(s) 2359
AciI CCGC 4 cut(s) 259, 1747, 2163, 2429
AclI AACGTT 1 cut(s) 1708
AclWI GGATC 4 cut(s) 443, 456, 1476, 2071
AcoI YGGCCR 1 cut(s) 2601
AcsI RAATTY 4 cut(s) 169, 676, 1328, 1882
AcuI CTGAAG 2 cut(s) 495, 2186
AcyI GRCGYC 2 cut(s) 2011, 2635
AdeI CACNNNGTG 1 cut(s) 2333
AfaI GTAC 4 cut(s) 138, 306, 937, 2621
AfiI CCNNNNNNNGG 3 cut(s) 78, 615, 2610
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 2 cut(s) 1212, 1591
AhlI ACTAGT 1 cut(s) 385
AjiI CACGTC 1 cut(s) 2543
AjuI GAANNNNNNNTTGG 2 cut(s) 2252, 2284
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 5 cut(s) 403, 425, 482, 709, 1883
AlwI GGATC 4 cut(s) 443, 456, 1476, 2071
Ama87I CYCGRG 1 cut(s) 2384
Aor13HI TCCGGA 1 cut(s) 2359
AoxI GGCC 1 cut(s) 2601
ApeKI GCWGC 4 cut(s) 738, 752, 1876, 2499
ApoI RAATTY 4 cut(s) 169, 676, 1328, 1882
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
AseI ATTAAT 1 cut(s) 731
Asp700I GAANNNNTTC 2 cut(s) 1011, 1584
AspLEI GCGC 1 cut(s) 1249
AspS9I GGNCC 3 cut(s) 787, 1897, 2228
AsuHPI GGTGA 5 cut(s) 91, 2008, 2218, 2531, 2560
AvaI CYCGRG 1 cut(s) 2384
AvaII GGWCC 3 cut(s) 787, 1897, 2228
BaeGI GKGCMC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 83
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 4 cut(s) 641, 1004, 1724, 2231
Bbv12I GWGCWC 1 cut(s) 462
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 739, 750, 1863, 2486
BccI CCATC 5 cut(s) 134, 617, 1405, 1685, 1874
BceAI ACGGC 3 cut(s) 31, 2367, 2622
BciVI GTATCC 2 cut(s) 1364, 2164
BclI TGATCA 1 cut(s) 1950
BcoDI GTCTC 5 cut(s) 403, 425, 482, 709, 1883
BcuI ACTAGT 1 cut(s) 385
BfaI CTAG 9 cut(s) 386, 582, 644, 927, 1443, 1505, 1569, 1679, 1754
BfmI CTRYAG 4 cut(s) 1149, 1982, 2304, 2315
BfrI CTTAAG 1 cut(s) 425
BfuAI ACCTGC 2 cut(s) 1252, 2106
BfuI GTATCC 2 cut(s) 1364, 2164
BglI GCCNNNNNGGC 1 cut(s) 2287
BglII AGATCT 3 cut(s) 66, 1445, 1756
BisI GCNGC 6 cut(s) 260, 739, 753, 1877, 2429, 2500
BlsI GCNGC 6 cut(s) 261, 740, 754, 1878, 2430, 2501
Bme18I GGWCC 3 cut(s) 787, 1897, 2228
BmeT110I CYCGRG 1 cut(s) 2384
BmgBI CACGTC 1 cut(s) 2543
BmgT120I GGNCC 3 cut(s) 787, 1897, 2228
BmiI GGNNCC 4 cut(s) 85, 450, 1350, 1946
BmsI GCATC 4 cut(s) 136, 459, 1255, 1734
BoxI GACNNNNGTC 1 cut(s) 210
BpiI GAAGAC 4 cut(s) 641, 1004, 1724, 2231
BplI GAGNNNNNCTC 4 cut(s) 479, 511, 1424, 1456
BpmI CTGGAG 1 cut(s) 1714
Bpu10I CCTNAGC 2 cut(s) 435, 444
BpuEI CTTGAG 4 cut(s) 1637, 1796, 1940, 1987
BsaHI GRCGYC 2 cut(s) 2011, 2635
BsaI GGTCTC 2 cut(s) 425, 1883
BsaJI CCNNGG 6 cut(s) 72, 463, 613, 1893, 1960, 2285
BsaWI WCCGGW 1 cut(s) 2359
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 615, 2610
Bse1I ACTGG 2 cut(s) 138, 246
Bse3DI GCAATG 5 cut(s) 18, 1268, 1347, 2246, 2440
BseAI TCCGGA 1 cut(s) 2359
BseDI CCNNGG 6 cut(s) 72, 463, 613, 1893, 1960, 2285
BseGI GGATG 7 cut(s) 474, 609, 781, 853, 1416, 2051, 2342
BseLI CCNNNNNNNGG 3 cut(s) 78, 615, 2610
BseMI GCAATG 5 cut(s) 18, 1268, 1347, 2246, 2440
BseMII CTCAG 3 cut(s) 435, 720, 1774
BseNI ACTGG 2 cut(s) 138, 246
BseRI GAGGAG 7 cut(s) 602, 686, 1082, 1140, 1793, 1937, 2537
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 4 cut(s) 739, 750, 1863, 2486
Bsh1236I CGCG 1 cut(s) 2629
BshFI GGCC 1 cut(s) 2603
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 2384
BsiSI CCGG 1 cut(s) 2360
BslFI GGGAC 3 cut(s) 369, 969, 1548
BslI CCNNNNNNNGG 3 cut(s) 78, 615, 2610
BsmAI GTCTC 5 cut(s) 403, 425, 482, 709, 1883
BsmFI GGGAC 3 cut(s) 369, 969, 1548
BsmI GAATGC 3 cut(s) 997, 2138, 2411
BsnI GGCC 1 cut(s) 2603
Bso31I GGTCTC 2 cut(s) 425, 1883
BsoBI CYCGRG 1 cut(s) 2384
Bsp1286I GDGCHC 2 cut(s) 88, 462
Bsp13I TCCGGA 1 cut(s) 2359
BspACI CCGC 4 cut(s) 259, 1747, 2163, 2429
BspANI GGCC 1 cut(s) 2603
BspCNI CTCAG 3 cut(s) 436, 719, 1773
BspEI TCCGGA 1 cut(s) 2359
BspFNI CGCG 1 cut(s) 2629
BspHI TCATGA 2 cut(s) 214, 1510
BspLI GGNNCC 4 cut(s) 85, 450, 1350, 1946
BspMAI CTGCAG 2 cut(s) 2308, 2319
BspMI ACCTGC 2 cut(s) 1252, 2106
BspPI GGATC 4 cut(s) 443, 456, 1476, 2071
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 2 cut(s) 425, 1883
BsrDI GCAATG 5 cut(s) 18, 1268, 1347, 2246, 2440
BsrI ACTGG 2 cut(s) 138, 246
BssECI CCNNGG 6 cut(s) 72, 463, 613, 1893, 1960, 2285
BssNI GRCGYC 2 cut(s) 2011, 2635
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 5 cut(s) 72, 463, 613, 1893, 2285
Bst2BI CACGAG 1 cut(s) 453
Bst4CI ACNGT 6 cut(s) 284, 1150, 1290, 1480, 1552, 2609
Bst6I CTCTTC 4 cut(s) 791, 1620, 2067, 2469
BstACI GRCGYC 2 cut(s) 2011, 2635
BstAFI CTTAAG 1 cut(s) 425
BstAPI GCANNNNNTGC 3 cut(s) 83, 2437, 2499
BstC8I GCNNGC 4 cut(s) 45, 911, 1160, 2138
BstDEI CTNAG 5 cut(s) 435, 444, 706, 1386, 1760
BstF5I GGATG 7 cut(s) 474, 609, 781, 853, 1416, 2051, 2342
BstFNI CGCG 1 cut(s) 2629
BstHHI GCGC 1 cut(s) 1249
BstMAI GTCTC 5 cut(s) 403, 425, 482, 709, 1883
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 440, 2287, 2437, 2499
BstNSI RCATGY 7 cut(s) 56, 997, 1033, 1162, 1216, 1595, 1725
BstPAI GACNNNNGTC 1 cut(s) 210
BstSFI CTRYAG 4 cut(s) 1149, 1982, 2304, 2315
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 1 cut(s) 2629
BstV1I GCAGC 4 cut(s) 739, 750, 1863, 2486
BstV2I GAAGAC 4 cut(s) 641, 1004, 1724, 2231
BstX2I RGATCY 6 cut(s) 66, 448, 1445, 1468, 1756, 2076
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 6 cut(s) 66, 448, 1445, 1468, 1756, 2076
BsuI GTATCC 2 cut(s) 1364, 2164
BsuRI GGCC 1 cut(s) 2603
BtrI CACGTC 1 cut(s) 2543
BtsCI GGATG 7 cut(s) 474, 609, 781, 853, 1416, 2051, 2342
BtsIMutI CAGTG 2 cut(s) 1533, 1768
BveI ACCTGC 2 cut(s) 1252, 2106
Cac8I GCNNGC 4 cut(s) 45, 911, 1160, 2138
CciI TCATGA 2 cut(s) 214, 1510
CfoI GCGC 1 cut(s) 1249
Cfr13I GGNCC 3 cut(s) 787, 1897, 2228
CseI GACGC 3 cut(s) 1922, 2019, 2573
Csp6I GTAC 4 cut(s) 137, 305, 936, 2620
CspCI CAANNNNNGTGG 6 cut(s) 1263, 1298, 1445, 1480, 1509, 1544
CviQI GTAC 4 cut(s) 137, 305, 936, 2620
DdeI CTNAG 5 cut(s) 435, 444, 706, 1386, 1760
DraI TTTAAA 1 cut(s) 1492
DraIII CACNNNGTG 1 cut(s) 2333
DrdI GACNNNNNNGTC 1 cut(s) 417
DseDI GACNNNNNNGTC 1 cut(s) 417
EaeI YGGCCR 1 cut(s) 2601
Eam1104I CTCTTC 4 cut(s) 791, 1620, 2067, 2469
EarI CTCTTC 4 cut(s) 791, 1620, 2067, 2469
Eco130I CCWWGG 5 cut(s) 72, 463, 613, 1893, 2285
Eco31I GGTCTC 2 cut(s) 425, 1883
Eco32I GATATC 2 cut(s) 577, 721
Eco47I GGWCC 3 cut(s) 787, 1897, 2228
Eco57I CTGAAG 2 cut(s) 495, 2186
Eco88I CYCGRG 1 cut(s) 2384
EcoO109I RGGNCCY 1 cut(s) 2228
EcoRI GAATTC 2 cut(s) 169, 1328
EcoRV GATATC 2 cut(s) 577, 721
EcoT14I CCWWGG 5 cut(s) 72, 463, 613, 1893, 2285
EcoT22I ATGCAT 3 cut(s) 999, 1270, 1727
ErhI CCWWGG 5 cut(s) 72, 463, 613, 1893, 2285
FalI AAGNNNNNCTT 2 cut(s) 1602, 1634
FaqI GGGAC 3 cut(s) 369, 969, 1548
FauI CCCGC 1 cut(s) 1740
FbaI TGATCA 1 cut(s) 1950
FblI GTMKAC 2 cut(s) 12, 276
Fnu4HI GCNGC 6 cut(s) 260, 739, 753, 1877, 2429, 2500
FokI GGATG 7 cut(s) 481, 596, 788, 860, 1423, 2058, 2349
Fsp4HI GCNGC 6 cut(s) 260, 739, 753, 1877, 2429, 2500
FspBI CTAG 9 cut(s) 386, 582, 644, 927, 1443, 1505, 1569, 1679, 1754
GlaI GCGC 1 cut(s) 1248
GluI GCNGC 6 cut(s) 260, 739, 753, 1877, 2429, 2500
GsuI CTGGAG 1 cut(s) 1714
HaeIII GGCC 1 cut(s) 2603
HapII CCGG 1 cut(s) 2360
HgaI GACGC 3 cut(s) 1922, 2019, 2573
HhaI GCGC 1 cut(s) 1249
Hin1I GRCGYC 2 cut(s) 2011, 2635
Hin6I GCGC 1 cut(s) 1247
HinP1I GCGC 1 cut(s) 1247
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 2198
HinfI GANTC 9 cut(s) 328, 494, 982, 1702, 1734, 1818, 2300, 2363, 2379
HpaII CCGG 1 cut(s) 2360
HphI GGTGA 5 cut(s) 91, 2008, 2218, 2531, 2560
Hpy166II GTNNAC 4 cut(s) 13, 277, 1307, 1359
Hpy188I TCNGA 7 cut(s) 377, 514, 709, 725, 1698, 1961, 2378
Hpy8I GTNNAC 4 cut(s) 13, 277, 1307, 1359
Hpy99I CGWCG 4 cut(s) 2567, 2609, 2634, 2637
HpyAV CCTTC 3 cut(s) 1001, 2105, 2219
HpyCH4III ACNGT 6 cut(s) 284, 1150, 1290, 1480, 1552, 2609
HpyCH4IV ACGT 7 cut(s) 30, 207, 219, 1708, 1988, 2236, 2542
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 440, 2287, 2437, 2499
HpyF3I CTNAG 5 cut(s) 435, 444, 706, 1386, 1760
HpySE526I ACGT 7 cut(s) 30, 207, 219, 1708, 1988, 2236, 2542
Hsp92I GRCGYC 2 cut(s) 2011, 2635
HspAI GCGC 1 cut(s) 1247
Kpn2I TCCGGA 1 cut(s) 2359
Ksp22I TGATCA 1 cut(s) 1950
LmnI GCTCC 9 cut(s) 465, 865, 923, 1069, 1155, 1167, 1218, 1780, 2414
Lsp1109I GCAGC 4 cut(s) 739, 750, 1863, 2486
LweI GCATC 4 cut(s) 136, 459, 1255, 1734
MaeI CTAG 9 cut(s) 386, 582, 644, 927, 1443, 1505, 1569, 1679, 1754
MaeII ACGT 7 cut(s) 30, 207, 219, 1708, 1988, 2236, 2542
MaeIII GTNAC 1 cut(s) 1910
MflI RGATCY 6 cut(s) 66, 448, 1445, 1468, 1756, 2076
MhlI GDGCHC 2 cut(s) 88, 462
MlyI GAGTC 3 cut(s) 488, 991, 2372
MmeI TCCRAC 3 cut(s) 305, 825, 2109
Mph1103I ATGCAT 3 cut(s) 999, 1270, 1727
MroI TCCGGA 1 cut(s) 2359
MroXI GAANNNNTTC 2 cut(s) 1011, 1584
MseI TTAA 9 cut(s) 342, 426, 563, 731, 759, 1056, 1491, 2058, 2513
MspCI CTTAAG 1 cut(s) 425
MspI CCGG 1 cut(s) 2360
Mva1269I GAATGC 3 cut(s) 997, 2138, 2411
MvnI CGCG 1 cut(s) 2629
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 440, 2287, 2437, 2499
NlaIV GGNNCC 4 cut(s) 85, 450, 1350, 1946
NmuCI GTSAC 1 cut(s) 1910
NsiI ATGCAT 3 cut(s) 999, 1270, 1727
NspI RCATGY 7 cut(s) 56, 997, 1033, 1162, 1216, 1595, 1725
PaeI GCATGC 1 cut(s) 1162
PaeR7I CTCGAG 1 cut(s) 2384
PagI TCATGA 2 cut(s) 214, 1510
PaqCI CACCTGC 1 cut(s) 2106
PciI ACATGT 2 cut(s) 1212, 1591
PctI GAATGC 3 cut(s) 997, 2138, 2411
PdmI GAANNNNTTC 2 cut(s) 1011, 1584
PfeI GAWTC 6 cut(s) 328, 1702, 1734, 1818, 2300, 2379
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 6 cut(s) 261, 740, 754, 1878, 2430, 2501
PleI GAGTC 3 cut(s) 488, 990, 2371
PpsI GAGTC 3 cut(s) 488, 990, 2371
PpuMI RGGWCCY 1 cut(s) 2228
PscI ACATGT 2 cut(s) 1212, 1591
PshAI GACNNNNGTC 1 cut(s) 210
PshBI ATTAAT 1 cut(s) 731
Psp1406I AACGTT 1 cut(s) 1708
Psp5II RGGWCCY 1 cut(s) 2228
PspN4I GGNNCC 4 cut(s) 85, 450, 1350, 1946
PspPI GGNCC 3 cut(s) 787, 1897, 2228
PspPPI RGGWCCY 1 cut(s) 2228
PspXI VCTCGAGB 1 cut(s) 2384
PstI CTGCAG 2 cut(s) 2308, 2319
PsuI RGATCY 6 cut(s) 66, 448, 1445, 1468, 1756, 2076
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 4 cut(s) 138, 306, 937, 2621
RsaNI GTAC 4 cut(s) 137, 305, 936, 2620
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 9 cut(s) 342, 426, 563, 731, 759, 1056, 1491, 2058, 2513
SatI GCNGC 6 cut(s) 260, 739, 753, 1877, 2429, 2500
Sau96I GGNCC 3 cut(s) 787, 1897, 2228
SchI GAGTC 3 cut(s) 488, 991, 2372
SduI GDGCHC 2 cut(s) 88, 462
SfaNI GCATC 4 cut(s) 136, 459, 1255, 1734
SfcI CTRYAG 4 cut(s) 1149, 1982, 2304, 2315
Sfr274I CTCGAG 1 cut(s) 2384
SinI GGWCC 3 cut(s) 787, 1897, 2228
SlaI CTCGAG 1 cut(s) 2384
SmlI CTYRAG 6 cut(s) 425, 1652, 1775, 1919, 1966, 2384
SmoI CTYRAG 6 cut(s) 425, 1652, 1775, 1919, 1966, 2384
SpeI ACTAGT 1 cut(s) 385
SphI GCATGC 1 cut(s) 1162
SsiI CCGC 4 cut(s) 259, 1747, 2163, 2429
SspI AATATT 1 cut(s) 1237
SspMI CTAG 9 cut(s) 386, 582, 644, 927, 1443, 1505, 1569, 1679, 1754
StyI CCWWGG 5 cut(s) 72, 463, 613, 1893, 2285
TaaI ACNGT 6 cut(s) 284, 1150, 1290, 1480, 1552, 2609
TaiI ACGT 7 cut(s) 33, 210, 222, 1711, 1991, 2239, 2545
TatI WGTACW 1 cut(s) 935
TauI GCSGC 2 cut(s) 262, 2431
TfiI GAWTC 6 cut(s) 328, 1702, 1734, 1818, 2300, 2379
Tru1I TTAA 9 cut(s) 342, 426, 563, 731, 759, 1056, 1491, 2058, 2513
Tru9I TTAA 9 cut(s) 342, 426, 563, 731, 759, 1056, 1491, 2058, 2513
TscAI CASTG 2 cut(s) 1533, 1768
TseFI GTSAC 1 cut(s) 1910
TseI GCWGC 4 cut(s) 738, 752, 1876, 2499
Tsp45I GTSAC 1 cut(s) 1910
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 2 cut(s) 1533, 1768
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 3 cut(s) 787, 1897, 2228
VspI ATTAAT 1 cut(s) 731
XapI RAATTY 4 cut(s) 169, 676, 1328, 1882
XbaI TCTAGA 1 cut(s) 1442
XceI RCATGY 7 cut(s) 56, 997, 1033, 1162, 1216, 1595, 1725
XhoI CTCGAG 1 cut(s) 2384
XmiI GTMKAC 2 cut(s) 12, 276
XmnI GAANNNNTTC 2 cut(s) 1011, 1584
XspI CTAG 9 cut(s) 386, 582, 644, 927, 1443, 1505, 1569, 1679, 1754
Zsp2I ATGCAT 3 cut(s) 999, 1270, 1727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.