Rroxscaffold_2G00126830

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000159
Physical Location & Seq
Forward (+)
62204541 .. 62242187
37647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126830.1

Sequence Viewer

Length: 2253 bp
ATGGAGTTTGCCAGCAGCATTGCAGAGAACGTCTTGGAAAGGCTAGCTTCACATGCTTCCCAAGAGATCTCCTTGGCACGGAGTGCCCAACTTGAGCTCACCAAGCTCGACAACACCTTATCTACCATCAAACTAGTGCTCGAGGATGCAGAGAAGAAGCAAGTGAGAAATCCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGATGTTTGCCATGACGTTGATGATGTCTTGGACAAACTAGACTTCCAAAAGTTGCGGTTGAAAGTGGAGGTCGACAACTGTGGGAAGATCAAAGGAAAGGTACGCCAATTTTTTTCCCGCTGGAATCCAGTACTGTTTAACTTCAGAATGGGACATAAAGTGAAAGAGATTAGAGAAAGACTAGTCGAAATTGATAATGAAAAGAGACAGTTTGCTCTCCTTGAGTTTGTTAAGATAGCCGAAGATCATCGTGCGCCCCAAGGGATGCACGATAATGAAAGAGAGACTGACTCTTTACTGGAGGCTACAGATGTTATTGGGAGAGATAATGATAAAAAACAGATTGTTAGCCATCTCTTAAATAACACTGATATCTCTACTGAGGAAAATGTTTGTGTTATTTCCATCCTTGGGTTAGGGGGGTTGGGAAAGACAACTCTTTCTAAATTAGTGTATAATGATAGGATGGTAGAGGAGAATTTTCAGATGAAAATGTGGATATGCGTTTCAGAAAACTTTGATATCCGAACATTAATTCGTGGCATTATCAATGCTGCAATTGGACAAAAATGTGAGGATGAAAGTTTGGACCTTATGAAAAAAAGGTTGCAAAATACTTTTAGAGATAGAAAGTTTTTGTTAGTGCTGGATGATGTCTGGGATACGGAGCTGATTGGAATAACGACTGAAAAATGGAATGATCTAAAAGGCTTGTTAAATGTGGGAGCTAATGGAAGTAAAATCATCATAACAACACGACATGGATTAGTTGCTTCACTTGTGAATCCCGTATACATGCACCCCTTAGAAGGTCTTCCCCACAAAGACTGCATCTCCTTGTTCATCAGAAGGGCATTTAAAAGAGGAGAGGAGCAACGCTATCAACATTTGATAGAAATTGGTGAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAGTAGCTACTGTAGGGAGCATGCTCTATTTGAATAGAGAGCAACACCATTGGTTGCGTGTCAGAGATGATGATATGTGGAGTATAGGAAATGACAATATTTTATCTGCACTCAAATTGAGCTACGATGCATTGCCACGACACTTGAAACCATGTTTTGCATTTTGTTCACTTTTCCCAAAGGATTATGAATTCAATAGTGAAATGTTGATTCCATTGTGGATGGCACAAGGCTACCTCAAGACATCTAAGAAAAATGAAGATTTGGATCAGATGGGTATAGACTATATAAGGCAGTGTTGCTCTAGGTCTTTGTTTCAAGTTGATATGGATCTCACAACATTTATAAGTTTTAAAATACATGATCTAGTACACGATTTAGCGATTTTGGTGGCACAAGTAGAGTACTCCACAGTCAATTTCCGTCCTTCTAGTGCTTTTGAAATGGTTCGACATATGTCAATATCCAAAAAGGACTTGCTTGGAGAAGAGGCACAAGTTCCCAATTTCATGCTCAAGTCAAAGAAATTGCGAACCATTCGAATTACTAGAGATGGTGAGATGAATCAACCTTTTGTGAAGACATGCATCTCGAGGTTCAAATATATGCGGGTGCTAGATATCGGTGGGTCGCCTCTCGAGGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATTTGAGATTCCTCGACTTGTCTGGTAATAGGAAGATAAAAAGGCTTCCCAATGCCATCAGTAAGCTGCTGAATATGGAGACCTTGTCCCTTGAGGATTGCGACGCACTTGAGGAGATACCCAAGGACATAGGGAACCTGATTAACCTACGAAGCTTGACGATAACAACCCAACAGACCTATTTGCCAAAAGGAATTAGACGCCTCACCTCACTTCAATCTTTAAGTTTTATTGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGTAAACTGGTGATTTGCAGTTGTGATAAGTTGGAATCCTTGCCACCAAATATGAAACACATGACTGCTTTAGATACTTTGTCTGTGGGTGATTGCGAGAAGCTTCAGTTGATGAGATCAGGGAAGGTCCTCGAGGTCTTCGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

750

Amino Acids

85.89

Weight (kDa)

7.02

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 93 2.5e-18 Rx N-terminal domain
NB-ARC PF00931 178 - 356 4.5e-29 NB-ARC domain
WHD_DRP PF23559 439 - 509 8.5e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 576 - 711 8.5e-24 Leucine-rich repeat region
LRR_RPS2 PF23247 602 - 734 7.9e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
AbsI CCTCGAGG 1 cut(s) 2234
AccI GTMKAC 2 cut(s) 276, 996
AciI CCGC 3 cut(s) 259, 322, 1753
AclWI GGATC 3 cut(s) 1419, 1482, 2077
AcsI RAATTY 2 cut(s) 682, 1334
AcuI CTGAAG 2 cut(s) 331, 2192
AcyI GRCGYC 1 cut(s) 2017
AdeI CACNNNGTG 1 cut(s) 83
AfaI GTAC 4 cut(s) 306, 336, 1515, 1550
AfiI CCNNNNNNNGG 3 cut(s) 78, 615, 1013
AgsI TTSAA 8 cut(s) 265, 1177, 1291, 1339, 1463, 1586, 1744, 2033
AhlI ACTAGT 2 cut(s) 133, 385
Alw21I GWGCWC 2 cut(s) 99, 141
Alw26I GTCTC 3 cut(s) 403, 482, 1889
AlwI GGATC 3 cut(s) 1419, 1482, 2077
Ama87I CYCGRG 4 cut(s) 140, 1735, 1781, 2234
ApeKI GCWGC 3 cut(s) 15, 758, 1882
ApoI RAATTY 2 cut(s) 682, 1334
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 1017, 1590
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 793, 2230
AsuHPI GGTGA 6 cut(s) 91, 1118, 1712, 2014, 2125, 2204
AsuII TTCGAA 1 cut(s) 1684
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 4 cut(s) 140, 1735, 1781, 2234
AvaII GGWCC 2 cut(s) 793, 2230
BaeGI GKGCMC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 3 cut(s) 1010, 1730, 2233
Bbv12I GWGCWC 2 cut(s) 99, 141
BbvI GCAGC 3 cut(s) 27, 745, 1869
BccI CCATC 8 cut(s) 134, 564, 617, 664, 1360, 1411, 1691, 1880
BciVI GTATCC 1 cut(s) 859
BcoDI GTCTC 3 cut(s) 403, 482, 1889
BcuI ACTAGT 2 cut(s) 133, 385
BfaI CTAG 9 cut(s) 44, 134, 242, 386, 1449, 1511, 1575, 1692, 1760
BfmI CTRYAG 2 cut(s) 510, 1155
BfuI GTATCC 1 cut(s) 859
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 16, 759, 1883
BlsI GCNGC 3 cut(s) 17, 760, 1884
BmcAI AGTACT 2 cut(s) 336, 1550
Bme18I GGWCC 2 cut(s) 793, 2230
BmeT110I CYCGRG 4 cut(s) 140, 1735, 1781, 2234
BmgT120I GGNCC 2 cut(s) 793, 2230
BmiI GGNNCC 1 cut(s) 1952
BmsI GCATC 5 cut(s) 136, 459, 1044, 1261, 1740
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1600
BpiI GAAGAC 3 cut(s) 1010, 1730, 2233
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 1684
BpuEI CTTGAG 6 cut(s) 113, 446, 1367, 1643, 1928, 1946
BsaBI GATNNNNATC 2 cut(s) 1410, 1473
BsaHI GRCGYC 1 cut(s) 2017
BsaI GGTCTC 1 cut(s) 1889
BsaJI CCNNGG 4 cut(s) 72, 463, 613, 1938
BsaXI ACNNNNNCTCC 4 cut(s) 1022, 1052, 1886, 1916
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 615, 1013
Bse1I ACTGG 3 cut(s) 332, 507, 2115
Bse3DI GCAATG 2 cut(s) 18, 1274
Bse8I GATNNNNATC 2 cut(s) 1410, 1473
BseDI CCNNGG 4 cut(s) 72, 463, 613, 1938
BseGI GGATG 8 cut(s) 151, 474, 609, 675, 787, 859, 1371, 2057
BseJI GATNNNNATC 2 cut(s) 1410, 1473
BseLI CCNNNNNNNGG 3 cut(s) 78, 615, 1013
BseMI GCAATG 2 cut(s) 18, 1274
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 3 cut(s) 332, 507, 2115
BseRI GAGGAG 6 cut(s) 692, 1083, 1088, 1146, 1799, 1943
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 3 cut(s) 27, 745, 1869
BsgI GTGCAG 1 cut(s) 1236
BsiHKAI GWGCWC 2 cut(s) 99, 141
BsiHKCI CYCGRG 4 cut(s) 140, 1735, 1781, 2234
BslFI GGGAC 2 cut(s) 369, 1888
BslI CCNNNNNNNGG 3 cut(s) 78, 615, 1013
BsmAI GTCTC 3 cut(s) 403, 482, 1889
BsmFI GGGAC 2 cut(s) 369, 1888
Bso31I GGTCTC 1 cut(s) 1889
BsoBI CYCGRG 4 cut(s) 140, 1735, 1781, 2234
Bsp119I TTCGAA 1 cut(s) 1684
Bsp1286I GDGCHC 3 cut(s) 88, 99, 141
BspACI CCGC 3 cut(s) 259, 322, 1753
BspCNI CTCAG 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 1952
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 1419, 1482, 2077
BspT104I TTCGAA 1 cut(s) 1684
BspTNI GGTCTC 1 cut(s) 1889
BsrDI GCAATG 2 cut(s) 18, 1274
BsrI ACTGG 3 cut(s) 332, 507, 2115
BssECI CCNNGG 4 cut(s) 72, 463, 613, 1938
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 1 cut(s) 2017
BssT1I CCWWGG 4 cut(s) 72, 463, 613, 1938
Bst1107I GTATAC 1 cut(s) 997
Bst4CI ACNGT 5 cut(s) 284, 339, 414, 1156, 1558
Bst6I CTCTTC 2 cut(s) 1626, 2073
BstACI GRCGYC 1 cut(s) 2017
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 1684
BstC8I GCNNGC 3 cut(s) 13, 45, 1166
BstDEI CTNAG 3 cut(s) 585, 1009, 1392
BstF5I GGATG 8 cut(s) 151, 474, 609, 675, 787, 859, 1371, 2057
BstHHI GCGC 1 cut(s) 460
BstMAI GTCTC 3 cut(s) 403, 482, 1889
BstMWI GCNNNNNNNGC 3 cut(s) 53, 83, 103
BstNSI RCATGY 4 cut(s) 56, 1003, 1168, 1731
BstPAI GACNNNNGTC 1 cut(s) 1600
BstSFI CTRYAG 2 cut(s) 510, 1155
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 3 cut(s) 27, 745, 1869
BstV2I GAAGAC 3 cut(s) 1010, 1730, 2233
BstX2I RGATCY 3 cut(s) 66, 1474, 2082
BstYI RGATCY 3 cut(s) 66, 1474, 2082
BstZ17I GTATAC 1 cut(s) 997
BsuI GTATCC 1 cut(s) 859
BtsCI GGATG 8 cut(s) 151, 474, 609, 675, 787, 859, 1371, 2057
BtsI GCAGTG 1 cut(s) 1445
BtsIMutI CAGTG 2 cut(s) 570, 1445
Cac8I GCNNGC 3 cut(s) 13, 45, 1166
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 793, 2230
CseI GACGC 2 cut(s) 1928, 2025
Csp6I GTAC 4 cut(s) 305, 335, 1514, 1549
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviQI GTAC 4 cut(s) 305, 335, 1514, 1549
DdeI CTNAG 3 cut(s) 585, 1009, 1392
DraI TTTAAA 2 cut(s) 1063, 1498
DraIII CACNNNGTG 1 cut(s) 83
Eam1104I CTCTTC 2 cut(s) 1626, 2073
EarI CTCTTC 2 cut(s) 1626, 2073
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 4 cut(s) 72, 463, 613, 1938
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 1889
Eco32I GATATC 3 cut(s) 577, 727, 1765
Eco47I GGWCC 2 cut(s) 793, 2230
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 331, 2192
Eco88I CYCGRG 4 cut(s) 140, 1735, 1781, 2234
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2230
EcoRI GAATTC 1 cut(s) 1334
EcoRV GATATC 3 cut(s) 577, 727, 1765
EcoT14I CCWWGG 4 cut(s) 72, 463, 613, 1938
EcoT22I ATGCAT 2 cut(s) 1276, 1733
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 4 cut(s) 72, 463, 613, 1938
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FaqI GGGAC 2 cut(s) 369, 1888
FauI CCCGC 2 cut(s) 329, 1746
FauNDI CATATG 1 cut(s) 1599
FblI GTMKAC 2 cut(s) 276, 996
Fnu4HI GCNGC 3 cut(s) 16, 759, 1883
FokI GGATG 8 cut(s) 158, 481, 596, 682, 794, 866, 1378, 2064
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 16, 759, 1883
FspBI CTAG 9 cut(s) 44, 134, 242, 386, 1449, 1511, 1575, 1692, 1760
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 16, 759, 1883
GsuI CTGGAG 1 cut(s) 524
HgaI GACGC 2 cut(s) 1928, 2025
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 1 cut(s) 2017
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 1969, 2204
HinfI GANTC 7 cut(s) 328, 494, 988, 1354, 1708, 1824, 2138
HphI GGTGA 6 cut(s) 91, 1118, 1712, 2014, 2125, 2204
Hpy166II GTNNAC 5 cut(s) 277, 997, 1313, 1516, 2108
Hpy188I TCNGA 7 cut(s) 350, 690, 715, 731, 1052, 1208, 1416
Hpy188III TCNNGA 3 cut(s) 1384, 1735, 1781
Hpy8I GTNNAC 5 cut(s) 277, 997, 1313, 1516, 2108
Hpy99I CGWCG 1 cut(s) 1922
HpyAV CCTTC 5 cut(s) 1007, 1047, 1581, 2111, 2221
HpyCH4III ACNGT 5 cut(s) 284, 339, 414, 1156, 1558
HpyCH4IV ACGT 2 cut(s) 30, 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 83, 103
HpyF3I CTNAG 3 cut(s) 585, 1009, 1392
HpySE526I ACGT 2 cut(s) 30, 219
Hsp92I GRCGYC 1 cut(s) 2017
HspAI GCGC 1 cut(s) 458
LmnI GCTCC 5 cut(s) 871, 929, 1075, 1161, 1786
Lsp1109I GCAGC 3 cut(s) 27, 745, 1869
LweI GCATC 5 cut(s) 136, 459, 1044, 1261, 1740
MaeI CTAG 9 cut(s) 44, 134, 242, 386, 1449, 1511, 1575, 1692, 1760
MaeII ACGT 2 cut(s) 30, 219
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 66, 1474, 2082
MhlI GDGCHC 3 cut(s) 88, 99, 141
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 2115
Mph1103I ATGCAT 2 cut(s) 1276, 1733
MroXI GAANNNNTTC 2 cut(s) 1017, 1590
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 324
MunI CAATTG 1 cut(s) 762
MwoI GCNNNNNNNGC 3 cut(s) 53, 83, 103
NdeI CATATG 1 cut(s) 1599
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 1952
NsiI ATGCAT 2 cut(s) 1276, 1733
NspI RCATGY 4 cut(s) 56, 1003, 1168, 1731
NspV TTCGAA 1 cut(s) 1684
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 4 cut(s) 140, 1735, 1781, 2234
PcsI WCGNNNNNNNCGW 1 cut(s) 2241
PdmI GAANNNNTTC 2 cut(s) 1017, 1590
PfeI GAWTC 6 cut(s) 328, 988, 1354, 1708, 1824, 2138
PflFI GACNNNGTC 1 cut(s) 1900
PkrI GCNGC 3 cut(s) 17, 760, 1884
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PpuMI RGGWCCY 1 cut(s) 2230
PshAI GACNNNNGTC 1 cut(s) 1600
PshBI ATTAAT 1 cut(s) 737
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 2230
PspN4I GGNNCC 1 cut(s) 1952
PspPI GGNCC 2 cut(s) 793, 2230
PspPPI RGGWCCY 1 cut(s) 2230
PspXI VCTCGAGB 2 cut(s) 140, 2234
PsuI RGATCY 3 cut(s) 66, 1474, 2082
PsyI GACNNNGTC 1 cut(s) 1900
RsaI GTAC 4 cut(s) 306, 336, 1515, 1550
RsaNI GTAC 4 cut(s) 305, 335, 1514, 1549
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 3 cut(s) 16, 759, 1883
Sau96I GGNCC 2 cut(s) 793, 2230
ScaI AGTACT 2 cut(s) 336, 1550
SchI GAGTC 1 cut(s) 488
SduI GDGCHC 3 cut(s) 88, 99, 141
SfaNI GCATC 5 cut(s) 136, 459, 1044, 1261, 1740
SfcI CTRYAG 2 cut(s) 510, 1155
Sfr274I CTCGAG 4 cut(s) 140, 1735, 1781, 2234
SfuI TTCGAA 1 cut(s) 1684
SinI GGWCC 2 cut(s) 793, 2230
SlaI CTCGAG 4 cut(s) 140, 1735, 1781, 2234
SmiMI CAYNNNNRTG 1 cut(s) 477
SpeI ACTAGT 2 cut(s) 133, 385
SphI GCATGC 1 cut(s) 1168
SsiI CCGC 3 cut(s) 259, 322, 1753
SspI AATATT 1 cut(s) 1243
SspMI CTAG 9 cut(s) 44, 134, 242, 386, 1449, 1511, 1575, 1692, 1760
SstI GAGCTC 1 cut(s) 99
StyI CCWWGG 4 cut(s) 72, 463, 613, 1938
TaaI ACNGT 5 cut(s) 284, 339, 414, 1156, 1558
TaiI ACGT 2 cut(s) 33, 222
TatI WGTACW 3 cut(s) 334, 1513, 1548
TfiI GAWTC 6 cut(s) 328, 988, 1354, 1708, 1824, 2138
TscAI CASTG 2 cut(s) 577, 1445
TseI GCWGC 3 cut(s) 15, 758, 1882
TspGWI ACGGA 3 cut(s) 94, 884, 1556
TspRI CASTG 2 cut(s) 577, 1445
Tth111I GACNNNGTC 1 cut(s) 1900
VpaK11BI GGWCC 2 cut(s) 793, 2230
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 2 cut(s) 682, 1334
XceI RCATGY 4 cut(s) 56, 1003, 1168, 1731
XhoI CTCGAG 4 cut(s) 140, 1735, 1781, 2234
XmiI GTMKAC 2 cut(s) 276, 996
XmnI GAANNNNTTC 2 cut(s) 1017, 1590
XspI CTAG 9 cut(s) 44, 134, 242, 386, 1449, 1511, 1575, 1692, 1760
ZrmI AGTACT 2 cut(s) 336, 1550
Zsp2I ATGCAT 2 cut(s) 1276, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.