RchiOBHm_Chr7g0180011

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000159
Physical Location & Seq
Forward (+)
2094716 .. 2098028
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16048

Sequence Viewer

Length: 2577 bp
ATGGAATTTGCCACTAGTGTTGCAGAGAGCGTCTTGGGTAGGCTAGCCTCTTATGCTGCCCAAGAAATCTGCTTGGCATGGGGTGCCCAACTTGAGTTGCAAAAGCTCAAGGAGACCTTGTTAGACATCAAAGACTTCCTCCAGGATGCGGAGGAAAAGCAAGTGAAGAATCCTATAATCAGCCGTTGGTTAGGAGATGTCAGAGATGTTTGCAACGACGCTGAGGATGCACTGGACGAATATGAGTTCCGAAAGCTCAGACTCAAAGTGCTCATCAACGACCATTGGAGAGTCAAAAGAGAGGTACACCAATTCTTCTCCCATTGGAATCCCGTTGTGTTTAATTTCAAAATGGGTCATAAATTGAAACGCATTAGGGAGCGTCTAGTTCAAATTGATAATGATGGAAAGACAAAGTTTTCTCTCGTTCAACAAACTGAAGTTCTTCCAGCACCCTTGAGGCCAGATGGTAGTAAAAAGGAGACAGACTCTTTACTGGAAGGTCATGTTGTTGGAAGAGATGGTGATAAGGAAAAAATTATCCATCTCTTTGATGACACCCTTTCCTCCAGTGTCGATAATGTTTCAGTTATTTCCATCTTTGGGTTAGCAGGGTTGGGAAAAACCACACTAGCTAAATCAGTGTATAAAGATACTGCGGTGGAGAAGAAATTTGATATTAGAATGTGGGTATGCGTCTCAGAGAACTTTAATATCCATACATTAGTTCAAGGCATTATTGCTGCAACTAAACAAAAATGTGCTAGTGAAAGTTTAGATCTTATGAAAAGGGGGTTGCAAGATATTTTGAGAGATAAAAAGTTTTTATTAGTGTTGGACGATGTCTGGGATATAGAGTCGATAGGAGTTACTAATCAAAAATGGAGTGAGTTAAAAACTTTGTTAGATGTTGGAGCCAAGGTGAGTAAAATCATCGTAACAACACGTAATGAATCAGTAGCTTCACTTGTGCAACCTATACATAGGCATCAGTTAGAAGGCCTTTCTCGTGAGGATTCCATGACTTTGTTTAAAAAAAATGCATTTAACAAAGGAGAGGAGTCACACTATCAACACTTGATAAAAATTGGAGAAGACATTGTGAAGAAGTGTGGAGGGGTTCCCTTAGCACTAGCTACTCTAGGGAGTTTACTTCACTCGGAAAGGGAGCGACGTCAGTGGTTACATGTAAGAGATAATGAAATATGGAGTTTGCCTGAAAATGACAACATTATGGCTGCACTAAAATTGAGCTACAATGCACTGCCACCTCATTTGAAGCCTTGTTTTGCCTTTTGTTCACTTTTCCCCAAGGATTATCAATTTTTAAGTGAAGAAATAATCTCATTATGGATGGCACAAGGCTTCCTCAAGTCCTTGAGAGAAAATGAAGATTTTACCGAGATGGGCCTCGACTATATTAGGCAGTTTTGTTCCAAGTCTTTGTTTCAACTTGAAGTTGATTGTAAGACTAGTGTGCAGTTCAAAATCCATGATTTAGTGCATGACTTGGCAATATCAGTGGCACGGGTAGATTGCTCCTCAATTAATTTCCGTCCCACTAGTGCTTTTGAAAAAGTCCGACACGTGTCAATATCGGAAAAAGACTTGTCTGGGGATGTGGCAGGAGTCCCGGCATTCATTCTCCAGTCGGAGAAACTGCGGACCATTCTAAATGTAGATAGTGAAGCTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCGCTAGATGTGCGTCGTTCTACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAACCTGGATTTTAATGAGAAGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTGGCCGTTGTATGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAATCTCAGATATTTGTATATAACAACACAGCAGATGTATTTTCCGAAAGGAGTATTTAGACGCCTCACCTTACTTCAATCCTTGTTCATTGGGACTTGTGCGAATCTGAAATCTCTGGGAGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTCGATCGGTTTCTGTGGAAATCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCACTCCATACTTTGGGGATCGTTAATTGTAAGAAGCTTGAGTTAATGAGGTCGGGAGAAGGCATCAATGGTCTCAGATCATTCGCTGTCATCATATCAGATTTGGAGGCTTTGCCCAATTGGCTTCAAGAGTCTGCAGACACTTTACAGAGCATGCACATCTCGAAGTGTGATAATCTCACGGCACTTCCAGAGTGGTTACAAAATCTCACCTTGCTGGAGCAACTGACGATTGGGGACTGCCCCAAATTGTCTGCTTTGCCGCAAGGGATGCATCGCCTCACTGCCTTGAGAAAATTGGGCATTGCCGGGTGTCCTGAATTGAGTAAAAGATGTAAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAACATGTCTCAAAGATTGTACTCGACGGAAGATGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

858

Amino Acids

97.45

Weight (kDa)

8.1

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 96 2.1e-22 Rx N-terminal domain
NB-ARC PF00931 186 - 351 5.2e-34 NB-ARC domain
AAA_22 PF13401 196 - 312 3.6e-06 AAA domain
WHD_DRP PF23559 435 - 505 6.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 576 - 776 1.9e-26 Leucine-rich repeat region
LRR_13 PF23286 590 - 737 4.6e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1177
AccB1I GGYRCC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 2179
AciI CCGC 4 cut(s) 149, 659, 1663, 2429
AclWI GGATC 3 cut(s) 1703, 2074, 2192
AcoI YGGCCR 2 cut(s) 1903, 2570
AcsI RAATTY 3 cut(s) 5, 671, 1882
AcuI CTGAAG 1 cut(s) 459
AcvI CACGTG 1 cut(s) 1588
AcyI GRCGYC 2 cut(s) 1174, 2014
AfaI GTAC 2 cut(s) 306, 2556
AfiI CCNNNNNNNGG 4 cut(s) 148, 603, 1912, 2179
AflII CTTAAG 1 cut(s) 1894
AflIII ACRYGT 5 cut(s) 944, 1186, 1585, 1587, 2539
AhlI ACTAGT 3 cut(s) 14, 1472, 1562
AjnI CCWGG 2 cut(s) 141, 1827
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 5 cut(s) 107, 476, 703, 2243, 2548
AlwI GGATC 3 cut(s) 1703, 2074, 2192
AoxI GGCC 5 cut(s) 461, 1000, 1408, 1903, 2570
ApeKI GCWGC 3 cut(s) 56, 743, 1238
ApoI RAATTY 3 cut(s) 5, 671, 1882
AseI ATTAAT 1 cut(s) 1548
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 1753
AspS9I GGNCC 3 cut(s) 1408, 1665, 2526
AsuC2I CCSGG 2 cut(s) 1634, 2476
AsuHPI GGTGA 4 cut(s) 536, 934, 2011, 2368
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 2 cut(s) 1665, 2526
BaeGI GKGCMC 1 cut(s) 88
BaeI ACNNNNGTAYC 2 cut(s) 645, 678
BalI TGGCCA 1 cut(s) 2572
BanI GGYRCC 1 cut(s) 83
BauI CACGAG 1 cut(s) 1008
BbrPI CACGTG 1 cut(s) 1588
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 43, 730, 1225
BccI CCATC 8 cut(s) 398, 461, 515, 552, 605, 1348, 1399, 2562
BceAI ACGGC 3 cut(s) 168, 1890, 2364
BciT130I CCWGG 2 cut(s) 143, 1829
BclI TGATCA 1 cut(s) 1950
BcnI CCSGG 2 cut(s) 1634, 2476
BcoDI GTCTC 5 cut(s) 107, 476, 703, 2243, 2548
BcuI ACTAGT 3 cut(s) 14, 1472, 1562
BfmI CTRYAG 1 cut(s) 2301
BfrI CTTAAG 1 cut(s) 1894
BglI GCCNNNNNGGC 1 cut(s) 2287
BglII AGATCT 1 cut(s) 778
BisI GCNGC 4 cut(s) 57, 744, 1239, 2429
BlsI GCNGC 4 cut(s) 58, 745, 1240, 2430
Bme1390I CCNGG 4 cut(s) 143, 1634, 1829, 2476
Bme18I GGWCC 2 cut(s) 1665, 2526
BmgT120I GGNCC 3 cut(s) 1408, 1665, 2526
BmiI GGNNCC 4 cut(s) 85, 916, 1122, 1946
BmrFI CCNGG 4 cut(s) 143, 1634, 1829, 2476
BmrI ACTGGG 1 cut(s) 1854
BmsI GCATC 7 cut(s) 136, 217, 997, 1734, 2238, 2427, 2449
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1854
BoxI GACNNNNGTC 1 cut(s) 1588
BpiI GAAGAC 1 cut(s) 1101
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 5 cut(s) 125, 553, 1631, 2153, 2405
Bpu10I CCTNAGC 2 cut(s) 222, 1126
BpuMI CCSGG 2 cut(s) 1634, 2476
BsaAI YACGTR 2 cut(s) 947, 1588
BsaHI GRCGYC 2 cut(s) 1174, 2014
BsaI GGTCTC 2 cut(s) 107, 2243
BsaJI CCNNGG 3 cut(s) 918, 1311, 1900
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 906, 936
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 603, 1912, 2179
Bse1I ACTGG 5 cut(s) 237, 501, 570, 1648, 1860
Bse3DI GCAATG 1 cut(s) 2469
BseBI CCWGG 2 cut(s) 143, 1829
BseDI CCNNGG 3 cut(s) 918, 1311, 1900
BseGI GGATG 5 cut(s) 151, 232, 1359, 1624, 2442
BseLI CCNNNNNNNGG 4 cut(s) 148, 603, 1912, 2179
BseMI GCAATG 1 cut(s) 2469
BseMII CTCAG 5 cut(s) 213, 271, 714, 1972, 2254
BseNI ACTGG 5 cut(s) 237, 501, 570, 1648, 1860
BseRI GAGGAG 4 cut(s) 1073, 1531, 1793, 1937
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 3 cut(s) 43, 730, 1225
BseYI CCCAGC 1 cut(s) 1691
BsgI GTGCAG 3 cut(s) 1224, 1499, 2151
Bsh1285I CGRYCG 1 cut(s) 2115
BshFI GGCC 5 cut(s) 463, 1002, 1410, 1905, 2572
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 2115
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 1634, 2475
BslFI GGGAC 4 cut(s) 1542, 1616, 2059, 2417
BslI CCNNNNNNNGG 4 cut(s) 148, 603, 1912, 2179
BsmAI GTCTC 5 cut(s) 107, 476, 703, 2243, 2548
BsmBI CGTCTC 1 cut(s) 703
BsmFI GGGAC 4 cut(s) 1542, 1616, 2059, 2417
BsmI GAATGC 1 cut(s) 1637
BsnI GGCC 5 cut(s) 463, 1002, 1410, 1905, 2572
Bso31I GGTCTC 2 cut(s) 107, 2243
Bsp1286I GDGCHC 2 cut(s) 88, 273
Bsp143I GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
BspACI CCGC 4 cut(s) 149, 659, 1663, 2429
BspANI GGCC 5 cut(s) 463, 1002, 1410, 1905, 2572
BspCNI CTCAG 5 cut(s) 214, 270, 713, 1971, 2253
BspLI GGNNCC 4 cut(s) 85, 916, 1122, 1946
BspMAI CTGCAG 1 cut(s) 2305
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 1703, 2074, 2192
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 1894
BspTNI GGTCTC 2 cut(s) 107, 2243
BsrDI GCAATG 1 cut(s) 2469
BsrI ACTGG 5 cut(s) 237, 501, 570, 1648, 1860
BssECI CCNNGG 3 cut(s) 918, 1311, 1900
BssMI GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
BssNI GRCGYC 2 cut(s) 1174, 2014
BssSI CACGAG 1 cut(s) 1008
BssT1I CCWWGG 3 cut(s) 918, 1311, 1900
Bst2BI CACGAG 1 cut(s) 1008
Bst2UI CCWGG 2 cut(s) 143, 1829
Bst4CI ACNGT 1 cut(s) 2109
Bst6I CTCTTC 2 cut(s) 511, 2070
BstACI GRCGYC 2 cut(s) 1174, 2014
BstAFI CTTAAG 1 cut(s) 1894
BstAPI GCANNNNNTGC 2 cut(s) 83, 2437
BstBAI YACGTR 2 cut(s) 947, 1588
BstC8I GCNNGC 4 cut(s) 45, 1749, 1874, 2321
BstDEI CTNAG 6 cut(s) 222, 257, 700, 1126, 1958, 2240
BstF5I GGATG 5 cut(s) 151, 232, 1359, 1624, 2442
BstHHI GCGC 1 cut(s) 1753
BstKTI GATC 8 cut(s) 781, 1698, 1953, 2082, 2115, 2187, 2246, 2535
BstMAI GTCTC 5 cut(s) 107, 476, 703, 2243, 2548
BstMBI GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
BstMCI CGRYCG 1 cut(s) 2115
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 227, 1759, 2287, 2437
BstNI CCWGG 2 cut(s) 143, 1829
BstNSI RCATGY 4 cut(s) 1190, 1725, 2323, 2543
BstPAI GACNNNNGTC 1 cut(s) 1588
BstSCI CCNGG 4 cut(s) 141, 1632, 1827, 2474
BstSFI CTRYAG 1 cut(s) 2301
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 3 cut(s) 43, 730, 1225
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 3 cut(s) 778, 1695, 2079
BstYI RGATCY 3 cut(s) 778, 1695, 2079
BsuRI GGCC 5 cut(s) 463, 1002, 1410, 1905, 2572
BtgZI GCGATG 1 cut(s) 2426
BtsCI GGATG 5 cut(s) 151, 232, 1359, 1624, 2442
BtsI GCAGTG 2 cut(s) 1262, 2448
BtsIMutI CAGTG 7 cut(s) 230, 577, 648, 1184, 1262, 1527, 2448
Cac8I GCNNGC 4 cut(s) 45, 1749, 1874, 2321
CfoI GCGC 1 cut(s) 1753
Cfr13I GGNCC 3 cut(s) 1408, 1665, 2526
CseI GACGC 6 cut(s) 19, 227, 371, 685, 1751, 2022
Csp6I GTAC 2 cut(s) 305, 2555
CspCI CAANNNNNGTGG 5 cut(s) 36, 1257, 1292, 1503, 1538
CviQI GTAC 2 cut(s) 305, 2555
DdeI CTNAG 6 cut(s) 222, 257, 700, 1126, 1958, 2240
DpnI GATC 8 cut(s) 780, 1697, 1952, 2081, 2114, 2186, 2245, 2534
DpnII GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
DraI TTTAAA 1 cut(s) 1033
EaeI YGGCCR 2 cut(s) 1903, 2570
Eam1104I CTCTTC 2 cut(s) 511, 2070
EarI CTCTTC 2 cut(s) 511, 2070
Eco130I CCWWGG 3 cut(s) 918, 1311, 1900
Eco147I AGGCCT 1 cut(s) 1002
Eco31I GGTCTC 2 cut(s) 107, 2243
Eco32I GATATC 1 cut(s) 1820
Eco47I GGWCC 2 cut(s) 1665, 2526
Eco57I CTGAAG 1 cut(s) 459
Eco72I CACGTG 1 cut(s) 1588
EcoRII CCWGG 2 cut(s) 141, 1827
EcoRV GATATC 1 cut(s) 1820
EcoT14I CCWWGG 3 cut(s) 918, 1311, 1900
EcoT22I ATGCAT 3 cut(s) 1045, 1727, 2442
ErhI CCWWGG 3 cut(s) 918, 1311, 1900
Esp3I CGTCTC 1 cut(s) 703
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 4 cut(s) 1542, 1616, 2059, 2417
FbaI TGATCA 1 cut(s) 1950
Fnu4HI GCNGC 4 cut(s) 57, 744, 1239, 2429
FokI GGATG 5 cut(s) 158, 239, 1366, 1631, 2449
Fsp4HI GCNGC 4 cut(s) 57, 744, 1239, 2429
GlaI GCGC 1 cut(s) 1752
GluI GCNGC 4 cut(s) 57, 744, 1239, 2429
GsaI CCCAGC 1 cut(s) 1695
GsuI CTGGAG 5 cut(s) 125, 553, 1631, 2153, 2405
HaeIII GGCC 5 cut(s) 463, 1002, 1410, 1905, 2572
HapII CCGG 2 cut(s) 1634, 2475
HgaI GACGC 6 cut(s) 19, 227, 371, 685, 1751, 2022
HhaI GCGC 1 cut(s) 1753
Hin1I GRCGYC 2 cut(s) 1174, 2014
Hin6I GCGC 1 cut(s) 1751
HinP1I GCGC 1 cut(s) 1751
HincII GTYRAC 1 cut(s) 2105
HindII GTYRAC 1 cut(s) 2105
HindIII AAGCTT 1 cut(s) 2201
HpaII CCGG 2 cut(s) 1634, 2475
HphI GGTGA 4 cut(s) 536, 934, 2011, 2368
Hpy166II GTNNAC 4 cut(s) 307, 1151, 1301, 2105
Hpy8I GTNNAC 4 cut(s) 307, 1151, 1301, 2105
Hpy99I CGWCG 4 cut(s) 221, 1176, 1767, 2564
HpyAV CCTTC 4 cut(s) 494, 992, 2108, 2219
HpyCH4III ACNGT 1 cut(s) 2109
HpyCH4IV ACGT 3 cut(s) 946, 1174, 1587
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 227, 1759, 2287, 2437
HpyF3I CTNAG 6 cut(s) 222, 257, 700, 1126, 1958, 2240
HpySE526I ACGT 3 cut(s) 946, 1174, 1587
Hsp92I GRCGYC 2 cut(s) 1174, 2014
HspAI GCGC 1 cut(s) 1751
Ksp22I TGATCA 1 cut(s) 1950
Kzo9I GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
LmnI GCTCC 6 cut(s) 379, 914, 1168, 1544, 1780, 2386
Lsp1109I GCAGC 3 cut(s) 43, 730, 1225
LweI GCATC 7 cut(s) 136, 217, 997, 1734, 2238, 2427, 2449
MaeII ACGT 3 cut(s) 946, 1174, 1587
MaeIII GTNAC 7 cut(s) 868, 937, 1062, 1182, 1799, 1926, 2364
MalI GATC 8 cut(s) 780, 1697, 1952, 2081, 2114, 2186, 2245, 2534
MboI GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
MfeI CAATTG 1 cut(s) 2284
MflI RGATCY 3 cut(s) 778, 1695, 2079
MhlI GDGCHC 2 cut(s) 88, 273
MlsI TGGCCA 1 cut(s) 2572
MluNI TGGCCA 1 cut(s) 2572
MlyI GAGTC 8 cut(s) 255, 300, 482, 866, 1070, 1638, 2144, 2306
MmeI TCCRAC 5 cut(s) 493, 816, 892, 1606, 1632
Mox20I TGGCCA 1 cut(s) 2572
Mph1103I ATGCAT 3 cut(s) 1045, 1727, 2442
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 2572
Msp20I TGGCCA 1 cut(s) 2572
MspCI CTTAAG 1 cut(s) 1894
MspI CCGG 2 cut(s) 1634, 2475
MspR9I CCNGG 4 cut(s) 143, 1634, 1829, 2476
MunI CAATTG 1 cut(s) 2284
Mva1269I GAATGC 1 cut(s) 1637
MvaI CCWGG 2 cut(s) 143, 1829
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 227, 1759, 2287, 2437
NciI CCSGG 2 cut(s) 1634, 2476
NdeII GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 4 cut(s) 85, 916, 1122, 1946
NmuCI GTSAC 1 cut(s) 1062
NsiI ATGCAT 3 cut(s) 1045, 1727, 2442
NspI RCATGY 4 cut(s) 1190, 1725, 2323, 2543
PaeI GCATGC 1 cut(s) 2323
PceI AGGCCT 1 cut(s) 1002
PciI ACATGT 2 cut(s) 1186, 2539
PctI GAATGC 1 cut(s) 1637
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 7 cut(s) 169, 328, 953, 1016, 1734, 1807, 2056
PflFI GACNNNGTC 1 cut(s) 842
PflMI CCANNNNNTGG 1 cut(s) 2179
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 4 cut(s) 58, 745, 1240, 2430
Ple19I CGATCG 1 cut(s) 2115
PleI GAGTC 8 cut(s) 255, 299, 482, 865, 1069, 1637, 2143, 2305
PmaCI CACGTG 1 cut(s) 1588
PmlI CACGTG 1 cut(s) 1588
PpsI GAGTC 8 cut(s) 255, 299, 482, 865, 1069, 1637, 2143, 2305
Ppu21I YACGTR 2 cut(s) 947, 1588
PscI ACATGT 2 cut(s) 1186, 2539
PshAI GACNNNNGTC 1 cut(s) 1588
PshBI ATTAAT 1 cut(s) 1548
Psp6I CCWGG 2 cut(s) 141, 1827
PspCI CACGTG 1 cut(s) 1588
PspFI CCCAGC 1 cut(s) 1691
PspGI CCWGG 2 cut(s) 141, 1827
PspN4I GGNNCC 4 cut(s) 85, 916, 1122, 1946
PspPI GGNCC 3 cut(s) 1408, 1665, 2526
PstI CTGCAG 1 cut(s) 2305
PsuI RGATCY 3 cut(s) 778, 1695, 2079
PsyI GACNNNGTC 1 cut(s) 842
PvuI CGATCG 1 cut(s) 2115
RsaI GTAC 2 cut(s) 306, 2556
RsaNI GTAC 2 cut(s) 305, 2555
SatI GCNGC 4 cut(s) 57, 744, 1239, 2429
Sau3AI GATC 8 cut(s) 778, 1695, 1950, 2079, 2112, 2184, 2243, 2532
Sau96I GGNCC 3 cut(s) 1408, 1665, 2526
SchI GAGTC 8 cut(s) 255, 300, 482, 866, 1070, 1638, 2144, 2306
ScrFI CCNGG 4 cut(s) 143, 1634, 1829, 2476
SduI GDGCHC 2 cut(s) 88, 273
SfaNI GCATC 7 cut(s) 136, 217, 997, 1734, 2238, 2427, 2449
SfcI CTRYAG 1 cut(s) 2301
SinI GGWCC 2 cut(s) 1665, 2526
SpeI ACTAGT 3 cut(s) 14, 1472, 1562
SphI GCATGC 1 cut(s) 2323
SseBI AGGCCT 1 cut(s) 1002
SsiI CCGC 4 cut(s) 149, 659, 1663, 2429
SspI AATATT 1 cut(s) 1709
StuI AGGCCT 1 cut(s) 1002
StyD4I CCNGG 4 cut(s) 141, 1632, 1827, 2474
StyI CCWWGG 3 cut(s) 918, 1311, 1900
TaaI ACNGT 1 cut(s) 2109
TaiI ACGT 3 cut(s) 949, 1177, 1590
TaqI TCGA 6 cut(s) 576, 860, 1413, 2111, 2330, 2559
TatI WGTACW 1 cut(s) 2554
TauI GCSGC 1 cut(s) 2431
TfiI GAWTC 7 cut(s) 169, 328, 953, 1016, 1734, 1807, 2056
TscAI CASTG 7 cut(s) 237, 577, 648, 1184, 1269, 1527, 2455
TseFI GTSAC 1 cut(s) 1062
TseI GCWGC 3 cut(s) 56, 743, 1238
Tsp45I GTSAC 1 cut(s) 1062
TspDTI ATGAA 8 cut(s) 800, 966, 1215, 1404, 1630, 1799, 2029, 2168
TspGWI ACGGA 2 cut(s) 1544, 2577
TspRI CASTG 7 cut(s) 237, 577, 648, 1184, 1269, 1527, 2455
Tth111I GACNNNGTC 1 cut(s) 842
Van91I CCANNNNNTGG 1 cut(s) 2179
Vha464I CTTAAG 1 cut(s) 1894
VpaK11BI GGWCC 2 cut(s) 1665, 2526
VspI ATTAAT 1 cut(s) 1548
XapI RAATTY 3 cut(s) 5, 671, 1882
XceI RCATGY 4 cut(s) 1190, 1725, 2323, 2543
XmnI GAANNNNTTC 1 cut(s) 444
ZraI GACGTC 1 cut(s) 1175
Zsp2I ATGCAT 3 cut(s) 1045, 1727, 2442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.