Rw7G002450

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000159
Physical Location & Seq
Forward (+)
2000706 .. 2003690
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002450.1

Sequence Viewer

Length: 2226 bp
ATGGAGTTTGCCTCTGGCATTGCTGAGACAGTCTTGGTTAGGCTAGCCTCCTATGCTGCCCAAGAAATTTGCTTGGCTTGGGGTGCCCAACTTGAGCTCAAGAAGCTCAATAAGACCTTGTTAGCCATCAAACTTGTACTCCAGGATGCAGAGAGGAAGCAAATGCAGAACCCCCTCATCACTCATTGGCTAGGAAACCTCAAAGATGTTTGCAACGATGCTGAGGATGTACTCGACGAATTTGAGTTCCGAAAGCTCCGACTCGAAGTGCTCACCAACGACCACCGGAGTGTCAAAGCAAAGGTACACCAATTCTTCTCCCGTTGGAATCCAGTCGTGTTTAACTTTAAAATGGGTCATAAAATCAAAGAGATTAGAGAAAGACTAGATGAGATTGATGCTGAAAAGACAAACTTTTCTCTCATTCAAGTTGTTGAACTAGTTGATGATTGTGGTGAGCCCCAAAGGGCACATGTTAATAAAAGGGAGACAGACTCTTTACTGGAAGGTCATGTTGTTGGAAGAGATGATGATAAGGAAAAAATTATCCTTCGCTTTGATGACACCCTTTCCTCCAGTGTCGATAATGTTTCAGTCATTTCCATCTTTGGGTTAGCAGGGTTGGGAAAAACCACACTCGCTAAATCAGTGTATAATGATACTGCGGTGGAGAACAAATTTGATATTAGAATGTGGGTATGCGTCTCAGAAAACTTTGATATCCATACATTAGTTCGTGGCATTATTGCTGCAACTAAACAAGAATGTGCTGTTGAAAGTTTAGATCTTATGAAAAAGGGGTTGCAGGGTATTTTGAAAGATAAAAAGTTTTTGTTAGTGTTGGACGATGTATGGGATAAAGAGTCGGTGGGAGTGTCACATCGAAAATGGAGTGAGTTAAAAACTGTGTTACATGTTGGAGCCAAAGGGAGTAAAATCATCGTAACAACACGTAATGAATCAGTAGCTTCACTTGTGCACCCTATACATAAGCATAAGTTAGAAGGTCTTTCTCAGGAGGATTCCATGACTTTATTTAAAAACAATGCATTTAACAAAGGAGAGGAGTCAGACTATCAACACTTGATAAAAATTGGAGAAGACATTGTGAAGAAGTGTCGAGGGGTTCCCTTAGCACTAGCTACTCTAGGGAGTTTACTTCACTCAGAAAGGGAGCGACGTCGGTGGTTACATGTAAGAGATAATGAAATATGGAGTTTGCTCGAAAATGACAATATTATGGCTGCACTAAAATTGAGCTACAATGCACTGCCACCTCACTTGAAGCCTTGCTTTGCCTTATGTTCACTTTACCCCAAGGATTATTTATTTAAAAGTAGAGAAATAATCCAATTATGGATGGCACAAGGCTTCCTCAAGTCCTCAAGAGAAAATGAAGATTTTACCGAGATGGGCCTCGACTATATAAGGCAGTTTTGTTCCAAGTCTTTGTTTCAACTTGAAGGTGATTTGAAGACTATAGTGCATTTCAAAATCCATGATTTAGTGCATGACTTGGCAATATCAGTGGCACGGGTAGATTGCTCCTCAATTAATTTCCGTCCCTCTAGTGCTTTTGAAAAAGTCCGACACGTGTCAATATCGGGAAAAGACTTGTCTGGGGATGTGGCAGGAGTCCCGGCATTCATTCTCCAGTCGGAGAAACTGCGGACCATTCTAAATGTAGATAGTGAAGCTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCGCTAGATGTGCGTCGTTCTACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAACCTGGACGACAATGCAAAGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAACCTTGCCCGTTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAATCTCAGATATTTGTATATAACTACACAGCAGATGTATTTTCCGAAAGGAGTATTTAGACGCCTCACCTTACTTCAATCCTTGTTCATTTGGAATTGTGCGAATCTGAAATCTCTGGGAGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTGGATCGGTGAGTGTGAAATTCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCACTCCATACTTTGGCGATCGCTTATTGTGATATAATCAAGGTGTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

741

Amino Acids

84.62

Weight (kDa)

7.56

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 95 1.1e-20 Rx N-terminal domain
NB-ARC PF00931 187 - 353 6e-36 NB-ARC domain
WHD_DRP PF23559 437 - 507 3.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 732 1.1e-22 Leucine-rich repeat region
LRR_13 PF23286 592 - 716 4.6e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 2185
AciI CCGC 2 cut(s) 665, 1669
AclWI GGATC 3 cut(s) 1709, 2080, 2126
AcsI RAATTY 5 cut(s) 66, 239, 677, 1888, 2133
AcvI CACGTG 1 cut(s) 1594
AcyI GRCGYC 2 cut(s) 1180, 2020
AfaI GTAC 3 cut(s) 138, 231, 306
AfiI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
AflIII ACRYGT 6 cut(s) 472, 913, 950, 1192, 1591, 1593
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 2 cut(s) 141, 1833
AleI CACNNNNGTG 1 cut(s) 288
Alw21I GWGCWC 3 cut(s) 99, 273, 981
Alw26I GTCTC 3 cut(s) 20, 482, 709
Alw44I GTGCAC 1 cut(s) 977
AlwI GGATC 3 cut(s) 1709, 2080, 2126
AoxI GGCC 1 cut(s) 1414
ApaLI GTGCAC 1 cut(s) 977
ApeKI GCWGC 3 cut(s) 56, 749, 1244
ApoI RAATTY 5 cut(s) 66, 239, 677, 1888, 2133
AseI ATTAAT 1 cut(s) 1554
AsiSI GCGATCGC 1 cut(s) 2193
AspLEI GCGC 1 cut(s) 1759
AspS9I GGNCC 2 cut(s) 1414, 1671
AsuC2I CCSGG 1 cut(s) 1640
AsuHPI GGTGA 5 cut(s) 265, 467, 1478, 2017, 2135
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 1 cut(s) 1671
BaeGI GKGCMC 3 cut(s) 88, 472, 981
BaeI ACNNNNGTAYC 2 cut(s) 651, 684
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 2 cut(s) 99, 462
BbrPI CACGTG 1 cut(s) 1594
BbsI GAAGAC 2 cut(s) 1107, 1481
Bbv12I GWGCWC 3 cut(s) 99, 273, 981
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 43, 736, 1231
BccI CCATC 4 cut(s) 134, 611, 1354, 1405
BciT130I CCWGG 2 cut(s) 143, 1835
BclI TGATCA 1 cut(s) 1956
BcnI CCSGG 1 cut(s) 1640
BcoDI GTCTC 3 cut(s) 20, 482, 709
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 8 cut(s) 44, 191, 386, 440, 1139, 1148, 1569, 1760
BfmI CTRYAG 1 cut(s) 1479
BglII AGATCT 1 cut(s) 784
BisI GCNGC 3 cut(s) 57, 750, 1245
BlsI GCNGC 3 cut(s) 58, 751, 1246
Bme1390I CCNGG 3 cut(s) 143, 1640, 1835
Bme18I GGWCC 1 cut(s) 1671
BmgT120I GGNCC 2 cut(s) 1414, 1671
BmiI GGNNCC 4 cut(s) 85, 922, 1128, 1952
BmrFI CCNGG 3 cut(s) 143, 1640, 1835
BmrI ACTGGG 1 cut(s) 1860
BmsI GCATC 4 cut(s) 136, 208, 388, 1740
BmtI GCTAGC 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 1860
BoxI GACNNNNGTC 1 cut(s) 1594
BpiI GAAGAC 2 cut(s) 1107, 1481
BplI GAGNNNNNCTC 3 cut(s) 28, 479, 511
BpmI CTGGAG 4 cut(s) 125, 559, 1637, 2159
Bpu10I CCTNAGC 2 cut(s) 222, 1132
BpuEI CTTGAG 7 cut(s) 83, 113, 1361, 1369, 1756, 1802, 1946
BpuMI CCSGG 1 cut(s) 1640
BsaAI YACGTR 2 cut(s) 953, 1594
BsaHI GRCGYC 2 cut(s) 1180, 2020
BsaJI CCNNGG 1 cut(s) 1317
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
Bse1I ACTGG 5 cut(s) 332, 507, 576, 1654, 1866
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 143, 1835
BseDI CCNNGG 1 cut(s) 1317
BseGI GGATG 4 cut(s) 151, 232, 1365, 1630
BseLI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 6 cut(s) 15, 213, 720, 1028, 1179, 1978
BseNI ACTGG 5 cut(s) 332, 507, 576, 1654, 1866
BseRI GAGGAG 4 cut(s) 1079, 1537, 1799, 1943
BseSI GKGCMC 3 cut(s) 88, 472, 981
BseXI GCAGC 3 cut(s) 43, 736, 1231
BseYI CCCAGC 1 cut(s) 1697
BsgI GTGCAG 2 cut(s) 1230, 2157
Bsh1285I CGRYCG 1 cut(s) 2193
BshFI GGCC 1 cut(s) 1416
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 2193
BsiHKAI GWGCWC 3 cut(s) 99, 273, 981
BsiSI CCGG 2 cut(s) 286, 1640
BslFI GGGAC 2 cut(s) 1548, 1622
BslI CCNNNNNNNGG 3 cut(s) 609, 1918, 2185
BsmAI GTCTC 3 cut(s) 20, 482, 709
BsmBI CGTCTC 1 cut(s) 709
BsmFI GGGAC 2 cut(s) 1548, 1622
BsmI GAATGC 1 cut(s) 1643
BsnI GGCC 1 cut(s) 1416
Bsp1286I GDGCHC 6 cut(s) 88, 99, 273, 462, 472, 981
Bsp143I GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
BspACI CCGC 2 cut(s) 665, 1669
BspANI GGCC 1 cut(s) 1416
BspCNI CTCAG 6 cut(s) 16, 214, 719, 1027, 1178, 1977
BspLI GGNNCC 4 cut(s) 85, 922, 1128, 1952
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 1709, 2080, 2126
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 5 cut(s) 332, 507, 576, 1654, 1866
BssECI CCNNGG 1 cut(s) 1317
BssMI GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
BssNI GRCGYC 2 cut(s) 1180, 2020
BssT1I CCWWGG 1 cut(s) 1317
Bst2UI CCWGG 2 cut(s) 143, 1835
Bst4CI ACNGT 3 cut(s) 31, 907, 2115
Bst6I CTCTTC 2 cut(s) 517, 2076
BstACI GRCGYC 2 cut(s) 1180, 2020
BstBAI YACGTR 2 cut(s) 953, 1594
BstC8I GCNNGC 3 cut(s) 45, 1755, 1880
BstDEI CTNAG 7 cut(s) 24, 222, 706, 1014, 1132, 1165, 1964
BstF5I GGATG 4 cut(s) 151, 232, 1365, 1630
BstHHI GCGC 1 cut(s) 1759
BstKTI GATC 6 cut(s) 787, 1704, 1959, 2088, 2121, 2193
BstMAI GTCTC 3 cut(s) 20, 482, 709
BstMBI GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
BstMCI CGRYCG 1 cut(s) 2193
BstMWI GCNNNNNNNGC 4 cut(s) 53, 83, 103, 1765
BstNI CCWGG 2 cut(s) 143, 1835
BstNSI RCATGY 4 cut(s) 476, 917, 1196, 1731
BstPAI GACNNNNGTC 1 cut(s) 1594
BstSCI CCNGG 3 cut(s) 141, 1638, 1833
BstSFI CTRYAG 1 cut(s) 1479
BstSLI GKGCMC 3 cut(s) 88, 472, 981
BstV1I GCAGC 3 cut(s) 43, 736, 1231
BstV2I GAAGAC 2 cut(s) 1107, 1481
BstX2I RGATCY 3 cut(s) 784, 1701, 2085
BstYI RGATCY 3 cut(s) 784, 1701, 2085
BsuRI GGCC 1 cut(s) 1416
BtsCI GGATG 4 cut(s) 151, 232, 1365, 1630
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 4 cut(s) 583, 654, 1268, 1533
Cac8I GCNNGC 3 cut(s) 45, 1755, 1880
CfoI GCGC 1 cut(s) 1759
Cfr13I GGNCC 2 cut(s) 1414, 1671
CseI GACGC 3 cut(s) 691, 1757, 2028
Csp6I GTAC 3 cut(s) 137, 230, 305
CspCI CAANNNNNGTGG 4 cut(s) 1263, 1298, 1509, 1544
CviAII CATG 9 cut(s) 473, 512, 914, 1027, 1193, 1499, 1511, 1728, 2158
CviQI GTAC 3 cut(s) 137, 230, 305
DdeI CTNAG 7 cut(s) 24, 222, 706, 1014, 1132, 1165, 1964
DpnI GATC 6 cut(s) 786, 1703, 1958, 2087, 2120, 2192
DpnII GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
DraI TTTAAA 3 cut(s) 349, 1039, 1333
Eam1104I CTCTTC 2 cut(s) 517, 2076
EarI CTCTTC 2 cut(s) 517, 2076
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 1317
Eco24I GRGCYC 2 cut(s) 99, 462
Eco32I GATATC 2 cut(s) 721, 1826
Eco47I GGWCC 1 cut(s) 1671
Eco53kI GAGCTC 1 cut(s) 97
Eco72I CACGTG 1 cut(s) 1594
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 141, 1833
EcoRV GATATC 2 cut(s) 721, 1826
EcoT14I CCWWGG 1 cut(s) 1317
EcoT22I ATGCAT 2 cut(s) 1051, 1733
EcoT38I GRGCYC 2 cut(s) 99, 462
ErhI CCWWGG 1 cut(s) 1317
Esp3I CGTCTC 1 cut(s) 709
FaeI CATG 9 cut(s) 476, 515, 917, 1030, 1196, 1502, 1514, 1731, 2161
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 1277, 1309
FaqI GGGAC 2 cut(s) 1548, 1622
FatI CATG 9 cut(s) 472, 511, 913, 1026, 1192, 1498, 1510, 1727, 2157
FbaI TGATCA 1 cut(s) 1956
Fnu4HI GCNGC 3 cut(s) 57, 750, 1245
FokI GGATG 4 cut(s) 158, 239, 1372, 1637
FriOI GRGCYC 2 cut(s) 99, 462
Fsp4HI GCNGC 3 cut(s) 57, 750, 1245
FspBI CTAG 8 cut(s) 44, 191, 386, 440, 1139, 1148, 1569, 1760
GlaI GCGC 1 cut(s) 1758
GluI GCNGC 3 cut(s) 57, 750, 1245
GsaI CCCAGC 1 cut(s) 1701
GsuI CTGGAG 4 cut(s) 125, 559, 1637, 2159
HaeIII GGCC 1 cut(s) 1416
HapII CCGG 2 cut(s) 286, 1640
HgaI GACGC 3 cut(s) 691, 1757, 2028
HhaI GCGC 1 cut(s) 1759
Hin1I GRCGYC 2 cut(s) 1180, 2020
Hin1II CATG 9 cut(s) 476, 515, 917, 1030, 1196, 1502, 1514, 1731, 2161
Hin6I GCGC 1 cut(s) 1757
HinP1I GCGC 1 cut(s) 1757
HincII GTYRAC 1 cut(s) 2111
HindII GTYRAC 1 cut(s) 2111
HpaII CCGG 2 cut(s) 286, 1640
HphI GGTGA 5 cut(s) 265, 467, 1478, 2017, 2135
Hpy166II GTNNAC 5 cut(s) 307, 979, 1157, 1307, 2111
Hpy188III TCNNGA 8 cut(s) 100, 1016, 1386, 1605, 1735, 1781, 1885, 2138
Hpy8I GTNNAC 5 cut(s) 307, 979, 1157, 1307, 2111
Hpy99I CGWCG 4 cut(s) 239, 1182, 1185, 1773
HpyAV CCTTC 5 cut(s) 500, 560, 998, 1457, 2114
HpyCH4III ACNGT 3 cut(s) 31, 907, 2115
HpyCH4IV ACGT 3 cut(s) 952, 1180, 1593
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 83, 103, 1765
HpyF3I CTNAG 7 cut(s) 24, 222, 706, 1014, 1132, 1165, 1964
HpySE526I ACGT 3 cut(s) 952, 1180, 1593
Hsp92I GRCGYC 2 cut(s) 1180, 2020
Hsp92II CATG 9 cut(s) 476, 515, 917, 1030, 1196, 1502, 1514, 1731, 2161
HspAI GCGC 1 cut(s) 1757
Ksp22I TGATCA 1 cut(s) 1956
Kzo9I GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
LmnI GCTCC 5 cut(s) 261, 920, 1174, 1550, 1786
Lsp1109I GCAGC 3 cut(s) 43, 736, 1231
LweI GCATC 4 cut(s) 136, 208, 388, 1740
MaeI CTAG 8 cut(s) 44, 191, 386, 440, 1139, 1148, 1569, 1760
MaeII ACGT 3 cut(s) 952, 1180, 1593
MaeIII GTNAC 6 cut(s) 876, 909, 943, 1188, 1805, 1932
MalI GATC 6 cut(s) 786, 1703, 1958, 2087, 2120, 2192
MboI GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
MboII GAAGA 8 cut(s) 307, 534, 1112, 1123, 1409, 1486, 2093, 2173
MflI RGATCY 3 cut(s) 784, 1701, 2085
MhlI GDGCHC 6 cut(s) 88, 99, 273, 462, 472, 981
MlyI GAGTC 6 cut(s) 255, 488, 872, 1076, 1644, 2150
MmeI TCCRAC 7 cut(s) 283, 305, 499, 822, 898, 1612, 1638
Mph1103I ATGCAT 2 cut(s) 1051, 1733
MslI CAYNNNNRTG 1 cut(s) 288
MspI CCGG 2 cut(s) 286, 1640
MspR9I CCNGG 3 cut(s) 143, 1640, 1835
Mva1269I GAATGC 1 cut(s) 1643
MvaI CCWGG 2 cut(s) 143, 1835
MwoI GCNNNNNNNGC 4 cut(s) 53, 83, 103, 1765
NciI CCSGG 1 cut(s) 1640
NdeII GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 9 cut(s) 476, 515, 917, 1030, 1196, 1502, 1514, 1731, 2161
NlaIV GGNNCC 4 cut(s) 85, 922, 1128, 1952
NmuCI GTSAC 1 cut(s) 876
NsiI ATGCAT 2 cut(s) 1051, 1733
NspI RCATGY 4 cut(s) 476, 917, 1196, 1731
OliI CACNNNNGTG 1 cut(s) 288
PciI ACATGT 3 cut(s) 472, 913, 1192
PctI GAATGC 1 cut(s) 1643
PfeI GAWTC 6 cut(s) 328, 959, 1022, 1740, 1813, 2062
PflMI CCANNNNNTGG 1 cut(s) 2185
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 3 cut(s) 58, 751, 1246
Ple19I CGATCG 1 cut(s) 2193
PleI GAGTC 6 cut(s) 255, 488, 871, 1075, 1643, 2149
PmaCI CACGTG 1 cut(s) 1594
PmlI CACGTG 1 cut(s) 1594
PpsI GAGTC 6 cut(s) 255, 488, 871, 1075, 1643, 2149
Ppu21I YACGTR 2 cut(s) 953, 1594
PscI ACATGT 3 cut(s) 472, 913, 1192
PshAI GACNNNNGTC 1 cut(s) 1594
PshBI ATTAAT 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 141, 1833
PspCI CACGTG 1 cut(s) 1594
PspFI CCCAGC 1 cut(s) 1697
PspGI CCWGG 2 cut(s) 141, 1833
PspN4I GGNNCC 4 cut(s) 85, 922, 1128, 1952
PspPI GGNCC 2 cut(s) 1414, 1671
PsuI RGATCY 3 cut(s) 784, 1701, 2085
PvuI CGATCG 1 cut(s) 2193
RgaI GCGATCGC 1 cut(s) 2193
RsaI GTAC 3 cut(s) 138, 231, 306
RsaNI GTAC 3 cut(s) 137, 230, 305
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 3 cut(s) 57, 750, 1245
Sau3AI GATC 6 cut(s) 784, 1701, 1956, 2085, 2118, 2190
Sau96I GGNCC 2 cut(s) 1414, 1671
SchI GAGTC 6 cut(s) 255, 488, 872, 1076, 1644, 2150
ScrFI CCNGG 3 cut(s) 143, 1640, 1835
SduI GDGCHC 6 cut(s) 88, 99, 273, 462, 472, 981
SfaAI GCGATCGC 1 cut(s) 2193
SfaNI GCATC 4 cut(s) 136, 208, 388, 1740
SfcI CTRYAG 1 cut(s) 1479
SgfI GCGATCGC 1 cut(s) 2193
SinI GGWCC 1 cut(s) 1671
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 7 cut(s) 92, 98, 1376, 1384, 1735, 1781, 1925
SmoI CTYRAG 7 cut(s) 92, 98, 1376, 1384, 1735, 1781, 1925
SpeI ACTAGT 1 cut(s) 439
SsiI CCGC 2 cut(s) 665, 1669
SspI AATATT 2 cut(s) 1237, 1715
SspMI CTAG 8 cut(s) 44, 191, 386, 440, 1139, 1148, 1569, 1760
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 141, 1638, 1833
StyI CCWWGG 1 cut(s) 1317
TaaI ACNGT 3 cut(s) 31, 907, 2115
TaiI ACGT 3 cut(s) 955, 1183, 1596
TaqI TCGA 7 cut(s) 234, 264, 582, 883, 1120, 1224, 1419
TatI WGTACW 2 cut(s) 136, 229
TfiI GAWTC 6 cut(s) 328, 959, 1022, 1740, 1813, 2062
TscAI CASTG 4 cut(s) 583, 654, 1275, 1533
TseFI GTSAC 1 cut(s) 876
TseI GCWGC 3 cut(s) 56, 749, 1244
Tsp45I GTSAC 1 cut(s) 876
TspDTI ATGAA 8 cut(s) 806, 972, 1221, 1410, 1636, 1805, 2035, 2174
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 4 cut(s) 583, 654, 1275, 1533
Van91I CCANNNNNTGG 1 cut(s) 2185
VneI GTGCAC 1 cut(s) 977
VpaK11BI GGWCC 1 cut(s) 1671
VspI ATTAAT 1 cut(s) 1554
XapI RAATTY 5 cut(s) 66, 239, 677, 1888, 2133
XceI RCATGY 4 cut(s) 476, 917, 1196, 1731
XspI CTAG 8 cut(s) 44, 191, 386, 440, 1139, 1148, 1569, 1760
ZraI GACGTC 1 cut(s) 1181
Zsp2I ATGCAT 2 cut(s) 1051, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.