Rw7G001730

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000159
Physical Location & Seq
Reverse (-)
1415650 .. 1418248
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001730.1

Sequence Viewer

Length: 2526 bp
ATGGGGGAGTTTGTCACTAGCATTGCACAGAACGCCGTGACTGGGCTAGCTTCTTTTACTTATAAAGAAGTTTGCTTGGCATGGGGTGTCACAGATGAGCTAGCCAAGCTCAAGGAAAAGCAGAGGAAGAATCCCCATCTGACCGAGTGGTTAGGCAAGCTCAAAGATGTATGCTACGACGTCGATGATGTGCTTGATGAATTTGCATTCAAAGAGCTACAAATGCAAGTGCTGAACAATCGTGGCAATATTGTAAAAGTAAAGGTACGCACATTCCTTTCGCGTTGGAACCCACTTGTGTTTAACATCAAAATGGCTCATAGAATCAAAGAGATTAGAGAAACGTTAGATAGAGTGGCAGCTGAAAAAGCTTCGTTTCCTCTTGAGGAACGAGCAGAAGATTGGCAGGGGGTGCACGTAAAGAGGGAGACTCACTCTTTTGTGCAAGTCTATGATGTTATTGGGAGAGAGGATGATAAAAAACAGATTATCATTCATCTCTTGAACTTAAGATTGAAAGATCCTGGTGTTCGTGAGAACGTTTCAGTTATTTCCATTTTGGGTTTAGGAGGTATGGGAAAGACCACCCTTGCCAAGTTGGTGTATAATGATAAAAGGGTAGTAAAAAACTTTGAGTTGAGAATGTGGGTATATGTCTCCGACCACTTTGATATTAAAAGATTGGTTCGAGAAATTATCACTTCTGCAACTAACCAAAACTGTGGGGTTGATGAAAGTTTAGAACAATTGCAAAAGAAGTTACAAAATGTTTTAACTGATAAACATTTTTTGTTAGTGTTAGATGATGTTTGGGACAAGAGTCAAATTGGAGTAACAATTTCAAAATGGCTTGATTTAAAAACTCTTTTGAATGTTGGAGCCAATGAGAGTAAAATCATTGTCACAACACGCAATGAATCGGTTACTTCGATTATGGGATCTGTACACGTGCATCAATTAAGAGGTCTTCGCCACGAAGATTGTATGTCCTTGTTCAGTCAAAGGGCATTTGGCCGAAGGGAAGAAGAGGAACGCTATCCAGAACTACTGGAAATCGGAGACGATATTGCAGAGAAGTGGAGCATGCTCTGTTTAAAAAGAGAACAGCATGCTTGGTCTGAAGTAAGAGATAGCGACATTTGGAAGCTGGAACAGGAGGACGACCACATATTTCCTGCCCTCAAATTGAGCTATGATGCATTGCCGCCCAACTTGAAACCATGCTTTGCCTTTTGCTCACTCTTTCCCAAAGACTACATATTCCGAAGTTCAGAATTGGTCCCCCTCTGGATGGCACATGGCTTTCTTCAATCTTCAAAAGGAAGCCAAGAGCCTGAAGAGATGGGACTAGACTACATAAGGCAGATATGTTCTAGATCATTGTTTCAAATTCACGAAGATTCAATTCATTTCATAGCCTTTGAAATGCATGATCTAGTTCATGACTTGGCCATCTCGGTGGCACAAGTAGAGTGCTCATCTGAAAATTTTCGCCCTTCTACCTCCAACCCTCACAAAAAAGTTCGACATGTGTCAATCTCGCGGGATGACTTGTGCATGAAGGAAGTACGAGTTCCTGATTACTTGCTTCAGCTGAAGAAAGCGTGGACCATTCTATTTCCAAATCCAGGAGATACAGTCATCACCAACAAACCTTTGTTGAAAACATGCATCAAGAGATTCAAATACCTGCGGGCCCTAGATTTGAGTGGTTGGAGTCTTGAAAAGTTACCAAGTTCAATTGGTAATTTGAGTCACTTGAGATATCTAGACTTGTCTAGGAATCCAAGCATGCAAAAGCTTCCGGATTCCATTTGCGAGTTGCAAAATTTGCAGACATTGCTACTTTCAGGCTGTGAGAATCTCAAAGGCTTGCCTAAAGACATAGGGAGCTTGCTCAACCTTAGAAAATTGGTGTTCACCACAAGTCGGATGGATTTGCCCGAAGAAATCAAAAGCCTCACTTCTCTTCGACATTTTGAGGTCCACAATTGCAGTAATCTTGAATCCTTAGGTGAAGTGGTACCATCTCTCACTAACCTTCGAATTCTTGTTATTAGCAACTGTGGAAATCTAGAGTCGCTGCCACACTTGAGTACACCATTAGAGGCCTTAGTCATCTCTGATTGTGAGAAGCTTGACTTGATGAGTTTGGGAGAAGGCATTCGAGGTCTTCGATCATTTTCCATCTCCAAAATGTCAAATTTAGAGGCTTTGCCCCATTGGCTTCAAGACTCCAAACCCAGTCTACAGAGCATACACATCGAAAATTGCGTGCATCTCAAGGCACTTCCAGAGTGGCTGCAAAGCTTGATGCTGCTGCAACAACTAGTCATCGAAGGTTGCCCTCAATTGTTGGCCTTGCCGGAGGGGATGCATCAACTCGCTGCCTTGAAAGAATTGAAGATAAATGACTGTTCTATATTGGCTGAAAGTTGCAGAAGGAAAGAAGGTCAGGACTGGTCCAAGATCACTCGTGTTGCTAAGATTACAATTGATGGAGAGATCGTTGCATCAAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

841

Amino Acids

96.08

Weight (kDa)

6.34

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 34 - 79 7e-10 Rx N-terminal domain
NB-ARC PF00931 178 - 340 7e-35 NB-ARC domain
WHD_DRP PF23559 414 - 484 1.6e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 556 - 756 5.6e-26 Leucine-rich repeat region
LRR_8 PF13855 564 - 619 4.2e-07 Leucine rich repeat
LRR_RPS2 PF23247 650 - 782 1.1e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 1698
Acc65I GGTACC 1 cut(s) 2023
AccB1I GGYRCC 1 cut(s) 2023
AccI GTMKAC 1 cut(s) 2248
AccII CGCG 2 cut(s) 283, 1543
AccIII TCCGGA 1 cut(s) 1804
AciI CCGC 3 cut(s) 1205, 1543, 1693
AclI AACGTT 2 cut(s) 344, 540
AclWI GGATC 2 cut(s) 515, 946
AcoI YGGCCR 2 cut(s) 1012, 1449
AcsI RAATTY 6 cut(s) 200, 1389, 1486, 1828, 2046, 2203
AcuI CTGAAG 4 cut(s) 1140, 1356, 1574, 1616
AcvI CACGTG 1 cut(s) 949
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 5 cut(s) 267, 945, 1569, 2025, 2098
AfiI CCNNNNNNNGG 4 cut(s) 42, 1291, 1628, 1929
AflII CTTAAG 1 cut(s) 508
AflIII ACRYGT 2 cut(s) 946, 1528
AhlI ACTAGT 1 cut(s) 2329
AjnI CCWGG 2 cut(s) 523, 1627
Alw21I GWGCWC 2 cut(s) 417, 1478
Alw26I GTCTC 3 cut(s) 422, 661, 1053
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 2 cut(s) 515, 946
Aor13HI TCCGGA 1 cut(s) 1804
AoxI GGCC 5 cut(s) 1012, 1449, 1695, 2109, 2358
ApaI GGGCCC 1 cut(s) 1699
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 6 cut(s) 359, 2083, 2302, 2317, 2320, 2387
ApoI RAATTY 6 cut(s) 200, 1389, 1486, 1828, 2046, 2203
Asp700I GAANNNNTTC 2 cut(s) 1488, 2163
Asp718I GGTACC 1 cut(s) 2023
AspS9I GGNCC 6 cut(s) 1279, 1608, 1695, 1696, 1984, 2463
AsuHPI GGTGA 3 cut(s) 1636, 1912, 2027
AsuII TTCGAA 1 cut(s) 2044
AsuNHI GCTAGC 2 cut(s) 46, 100
AvaII GGWCC 4 cut(s) 1279, 1608, 1984, 2463
AxyI CCTNAGG 1 cut(s) 2011
BaeGI GKGCMC 2 cut(s) 417, 1699
BalI TGGCCA 1 cut(s) 1451
BanI GGYRCC 1 cut(s) 2023
BanII GRGCYC 1 cut(s) 1699
BauI CACGAG 1 cut(s) 2475
BbrPI CACGTG 1 cut(s) 949
BbsI GAAGAC 2 cut(s) 959, 2165
Bbv12I GWGCWC 2 cut(s) 417, 1478
BbvI GCAGC 6 cut(s) 371, 2070, 2289, 2304, 2307, 2374
BccI CCATC 8 cut(s) 144, 1285, 1336, 1460, 1927, 2035, 2195, 2492
BceAI ACGGC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 2245, 2279
BciT130I CCWGG 2 cut(s) 525, 1629
BcoDI GTCTC 3 cut(s) 422, 661, 1053
BcuI ACTAGT 1 cut(s) 2329
BfmI CTRYAG 1 cut(s) 2249
BfrI CTTAAG 1 cut(s) 508
BfuAI ACCTGC 1 cut(s) 1698
BglI GCCNNNNNGGC 1 cut(s) 2224
BisI GCNGC 7 cut(s) 360, 1205, 2084, 2303, 2318, 2321, 2388
BlsI GCNGC 7 cut(s) 361, 1206, 2085, 2304, 2319, 2322, 2389
Bme1390I CCNGG 2 cut(s) 525, 1629
Bme18I GGWCC 4 cut(s) 1279, 1608, 1984, 2463
BmgT120I GGNCC 6 cut(s) 1279, 1608, 1695, 1696, 1984, 2463
BmiI GGNNCC 5 cut(s) 290, 880, 1281, 1697, 2025
BmrFI CCNGG 2 cut(s) 525, 1629
BmrI ACTGGG 2 cut(s) 51, 2238
BmsI GCATC 8 cut(s) 961, 1186, 1680, 2287, 2304, 2364, 2386, 2522
BmtI GCTAGC 2 cut(s) 50, 104
BmuI ACTGGG 2 cut(s) 51, 2238
BoxI GACNNNNGTC 2 cut(s) 819, 1531
BpiI GAAGAC 2 cut(s) 959, 2165
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 419, 451
Bpu14I TTCGAA 1 cut(s) 2044
BpuEI CTTGAG 5 cut(s) 95, 404, 1780, 2113, 2267
BsaAI YACGTR 2 cut(s) 418, 949
BsaHI GRCGYC 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 1804
BsaXI ACNNNNNCTCC 4 cut(s) 1623, 1653, 2493, 2523
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 1291, 1628, 1929
Bse1I ACTGG 4 cut(s) 46, 1053, 2244, 2465
Bse21I CCTNAGG 1 cut(s) 2011
Bse3DI GCAATG 4 cut(s) 21, 919, 1199, 1838
BseAI TCCGGA 1 cut(s) 1804
BseBI CCWGG 2 cut(s) 525, 1629
BseGI GGATG 5 cut(s) 478, 1296, 1552, 1938, 2379
BseLI CCNNNNNNNGG 4 cut(s) 42, 1291, 1628, 1929
BseMI GCAATG 4 cut(s) 21, 919, 1199, 1838
BseNI ACTGG 4 cut(s) 46, 1053, 2244, 2465
BseSI GKGCMC 2 cut(s) 417, 1699
BseXI GCAGC 6 cut(s) 371, 2070, 2289, 2304, 2307, 2374
Bsh1236I CGCG 2 cut(s) 283, 1543
BshFI GGCC 5 cut(s) 1014, 1451, 1697, 2111, 2360
BshNI GGYRCC 1 cut(s) 2023
BsiHKAI GWGCWC 2 cut(s) 417, 1478
BsiSI CCGG 2 cut(s) 1805, 2366
BslFI GGGAC 3 cut(s) 827, 1265, 1359
BslI CCNNNNNNNGG 4 cut(s) 42, 1291, 1628, 1929
BsmAI GTCTC 3 cut(s) 422, 661, 1053
BsmBI CGTCTC 1 cut(s) 1053
BsmFI GGGAC 3 cut(s) 827, 1265, 1359
BsmI GAATGC 2 cut(s) 206, 2163
BsnI GGCC 5 cut(s) 1014, 1451, 1697, 2111, 2360
Bsp119I TTCGAA 1 cut(s) 2044
Bsp120I GGGCCC 1 cut(s) 1695
Bsp1286I GDGCHC 3 cut(s) 417, 1478, 1699
Bsp13I TCCGGA 1 cut(s) 1804
Bsp1407I TGTACA 1 cut(s) 943
Bsp143I GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
BspACI CCGC 3 cut(s) 1205, 1543, 1693
BspANI GGCC 5 cut(s) 1014, 1451, 1697, 2111, 2360
BspEI TCCGGA 1 cut(s) 1804
BspFNI CGCG 2 cut(s) 283, 1543
BspHI TCATGA 1 cut(s) 1441
BspLI GGNNCC 5 cut(s) 290, 880, 1281, 1697, 2025
BspMI ACCTGC 1 cut(s) 1698
BspOI GCTAGC 2 cut(s) 50, 104
BspPI GGATC 2 cut(s) 515, 946
BspT104I TTCGAA 1 cut(s) 2044
BspT107I GGYRCC 1 cut(s) 2023
BspTI CTTAAG 1 cut(s) 508
BsrDI GCAATG 4 cut(s) 21, 919, 1199, 1838
BsrGI TGTACA 1 cut(s) 943
BsrI ACTGG 4 cut(s) 46, 1053, 2244, 2465
BssMI GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 2475
Bst2BI CACGAG 1 cut(s) 2475
Bst2UI CCWGG 2 cut(s) 525, 1629
Bst4CI ACNGT 4 cut(s) 722, 1639, 2066, 2417
Bst6I CTCTTC 3 cut(s) 1020, 1332, 1974
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 508
BstAPI GCANNNNNTGC 3 cut(s) 412, 1831, 1840
BstAUI TGTACA 1 cut(s) 943
BstBAI YACGTR 2 cut(s) 418, 949
BstBI TTCGAA 1 cut(s) 2044
BstDEI CTNAG 4 cut(s) 1904, 2011, 2113, 2484
BstF5I GGATG 5 cut(s) 478, 1296, 1552, 1938, 2379
BstFNI CGCG 2 cut(s) 283, 1543
BstKTI GATC 7 cut(s) 523, 941, 1379, 1435, 2180, 2472, 2508
BstMAI GTCTC 3 cut(s) 422, 661, 1053
BstMBI GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
BstMWI GCNNNNNNNGC 8 cut(s) 32, 106, 223, 368, 412, 1831, 1840, 2224
BstNI CCWGG 2 cut(s) 525, 1629
BstNSI RCATGY 5 cut(s) 1087, 1112, 1532, 1671, 1795
BstPAI GACNNNNGTC 2 cut(s) 819, 1531
BstSCI CCNGG 2 cut(s) 523, 1627
BstSFI CTRYAG 1 cut(s) 2249
BstSLI GKGCMC 2 cut(s) 417, 1699
BstUI CGCG 2 cut(s) 283, 1543
BstV1I GCAGC 6 cut(s) 371, 2070, 2289, 2304, 2307, 2374
BstV2I GAAGAC 2 cut(s) 959, 2165
BstX2I RGATCY 2 cut(s) 520, 938
BstXI CCANNNNNNTGG 2 cut(s) 722, 1459
BstYI RGATCY 2 cut(s) 520, 938
Bsu36I CCTNAGG 1 cut(s) 2011
BsuRI GGCC 5 cut(s) 1014, 1451, 1697, 2111, 2360
BtsCI GGATG 5 cut(s) 478, 1296, 1552, 1938, 2379
BveI ACCTGC 1 cut(s) 1698
CciI TCATGA 1 cut(s) 1441
Cfr13I GGNCC 6 cut(s) 1279, 1608, 1695, 1696, 1984, 2463
Csp6I GTAC 5 cut(s) 266, 944, 1568, 2024, 2097
CspCI CAANNNNNGTGG 2 cut(s) 962, 997
CviQI GTAC 5 cut(s) 266, 944, 1568, 2024, 2097
DdeI CTNAG 4 cut(s) 1904, 2011, 2113, 2484
DpnI GATC 7 cut(s) 522, 940, 1378, 1434, 2179, 2471, 2507
DpnII GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
DraI TTTAAA 2 cut(s) 858, 1095
EaeI YGGCCR 2 cut(s) 1012, 1449
Eam1104I CTCTTC 3 cut(s) 1020, 1332, 1974
EarI CTCTTC 3 cut(s) 1020, 1332, 1974
Eco147I AGGCCT 1 cut(s) 2111
Eco24I GRGCYC 1 cut(s) 1699
Eco32I GATATC 1 cut(s) 1766
Eco47I GGWCC 4 cut(s) 1279, 1608, 1984, 2463
Eco57I CTGAAG 4 cut(s) 1140, 1356, 1574, 1616
Eco72I CACGTG 1 cut(s) 949
Eco81I CCTNAGG 1 cut(s) 2011
EcoO109I RGGNCCY 1 cut(s) 1696
EcoRI GAATTC 1 cut(s) 2046
EcoRII CCWGG 2 cut(s) 523, 1627
EcoRV GATATC 1 cut(s) 1766
EcoT22I ATGCAT 4 cut(s) 1201, 1431, 1673, 2379
EcoT38I GRGCYC 1 cut(s) 1699
Esp3I CGTCTC 1 cut(s) 1053
FalI AAGNNNNNCTT 4 cut(s) 1948, 1980, 2126, 2158
FaqI GGGAC 3 cut(s) 827, 1265, 1359
FauI CCCGC 2 cut(s) 1536, 1686
FblI GTMKAC 1 cut(s) 2248
Fnu4HI GCNGC 7 cut(s) 360, 1205, 2084, 2303, 2318, 2321, 2388
FokI GGATG 5 cut(s) 485, 1303, 1559, 1945, 2386
FriOI GRGCYC 1 cut(s) 1699
Fsp4HI GCNGC 7 cut(s) 360, 1205, 2084, 2303, 2318, 2321, 2388
GluI GCNGC 7 cut(s) 360, 1205, 2084, 2303, 2318, 2321, 2388
HaeIII GGCC 5 cut(s) 1014, 1451, 1697, 2111, 2360
HapII CCGG 2 cut(s) 1805, 2366
Hin1I GRCGYC 1 cut(s) 180
HindIII AAGCTT 4 cut(s) 369, 1799, 2135, 2308
HpaII CCGG 2 cut(s) 1805, 2366
HphI GGTGA 3 cut(s) 1636, 1912, 2027
Hpy166II GTNNAC 7 cut(s) 415, 946, 1608, 1920, 1987, 2099, 2249
Hpy188I TCNGA 9 cut(s) 141, 661, 1058, 1120, 1265, 1273, 1483, 1932, 2125
Hpy8I GTNNAC 7 cut(s) 415, 946, 1608, 1920, 1987, 2099, 2249
Hpy99I CGWCG 2 cut(s) 182, 185
HpyAV CCTTC 8 cut(s) 1011, 1506, 1555, 2051, 2153, 2333, 2436, 2444
HpyCH4III ACNGT 4 cut(s) 722, 1639, 2066, 2417
HpyCH4IV ACGT 5 cut(s) 180, 344, 417, 540, 948
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 106, 223, 368, 412, 1831, 1840, 2224
HpyF3I CTNAG 4 cut(s) 1904, 2011, 2113, 2484
HpySE526I ACGT 5 cut(s) 180, 344, 417, 540, 948
Hsp92I GRCGYC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 1804
KpnI GGTACC 1 cut(s) 2027
Kzo9I GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
LmnI GCTCC 3 cut(s) 878, 1080, 1890
Lsp1109I GCAGC 6 cut(s) 371, 2070, 2289, 2304, 2307, 2374
LweI GCATC 8 cut(s) 961, 1186, 1680, 2287, 2304, 2364, 2386, 2522
MaeII ACGT 5 cut(s) 180, 344, 417, 540, 948
MaeIII GTNAC 9 cut(s) 13, 37, 88, 759, 832, 901, 922, 1728, 1754
MalI GATC 7 cut(s) 522, 940, 1378, 1434, 2179, 2471, 2507
MboI GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
MfeI CAATTG 5 cut(s) 746, 1740, 1990, 2351, 2493
MflI RGATCY 2 cut(s) 520, 938
MhlI GDGCHC 3 cut(s) 417, 1478, 1699
MlsI TGGCCA 1 cut(s) 1451
MluNI TGGCCA 1 cut(s) 1451
MlyI GAGTC 6 cut(s) 424, 829, 1726, 1762, 2087, 2228
MmeI TCCRAC 6 cut(s) 266, 684, 856, 1530, 1694, 1910
Mox20I TGGCCA 1 cut(s) 1451
Mph1103I ATGCAT 4 cut(s) 1201, 1431, 1673, 2379
MroI TCCGGA 1 cut(s) 1804
MroXI GAANNNNTTC 2 cut(s) 1488, 2163
MscI TGGCCA 1 cut(s) 1451
MseI TTAA 7 cut(s) 303, 509, 675, 773, 857, 959, 1094
MslI CAYNNNNRTG 2 cut(s) 311, 1457
Msp20I TGGCCA 1 cut(s) 1451
MspA1I CMGCKG 2 cut(s) 362, 1594
MspCI CTTAAG 1 cut(s) 508
MspI CCGG 2 cut(s) 1805, 2366
MspR9I CCNGG 2 cut(s) 525, 1629
MunI CAATTG 5 cut(s) 746, 1740, 1990, 2351, 2493
Mva1269I GAATGC 2 cut(s) 206, 2163
MvaI CCWGG 2 cut(s) 525, 1629
MvnI CGCG 2 cut(s) 283, 1543
MwoI GCNNNNNNNGC 8 cut(s) 32, 106, 223, 368, 412, 1831, 1840, 2224
NdeII GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
NheI GCTAGC 2 cut(s) 46, 100
NlaIV GGNNCC 5 cut(s) 290, 880, 1281, 1697, 2025
NmuCI GTSAC 5 cut(s) 13, 37, 88, 901, 1754
NsiI ATGCAT 4 cut(s) 1201, 1431, 1673, 2379
NspI RCATGY 5 cut(s) 1087, 1112, 1532, 1671, 1795
NspV TTCGAA 1 cut(s) 2044
PaeI GCATGC 3 cut(s) 1087, 1112, 1795
PagI TCATGA 1 cut(s) 1441
PceI AGGCCT 1 cut(s) 2111
PciI ACATGT 1 cut(s) 1528
PcsI WCGNNNNNNNCGW 3 cut(s) 926, 2173, 2271
PctI GAATGC 2 cut(s) 206, 2163
PdmI GAANNNNTTC 2 cut(s) 1488, 2163
PfeI GAWTC 9 cut(s) 130, 324, 917, 1400, 1680, 1783, 1808, 1861, 2006
PfoI TCCNGGA 1 cut(s) 1627
PkrI GCNGC 7 cut(s) 361, 1206, 2085, 2304, 2319, 2322, 2389
PleI GAGTC 6 cut(s) 424, 828, 1725, 1761, 2086, 2228
PmaCI CACGTG 1 cut(s) 949
PmlI CACGTG 1 cut(s) 949
PpsI GAGTC 6 cut(s) 424, 828, 1725, 1761, 2086, 2228
Ppu21I YACGTR 2 cut(s) 418, 949
PscI ACATGT 1 cut(s) 1528
PshAI GACNNNNGTC 2 cut(s) 819, 1531
PsiI TTATAA 1 cut(s) 63
Psp1406I AACGTT 2 cut(s) 344, 540
Psp6I CCWGG 2 cut(s) 523, 1627
PspCI CACGTG 1 cut(s) 949
PspGI CCWGG 2 cut(s) 523, 1627
PspN4I GGNNCC 5 cut(s) 290, 880, 1281, 1697, 2025
PspOMI GGGCCC 1 cut(s) 1695
PspPI GGNCC 6 cut(s) 1279, 1608, 1695, 1696, 1984, 2463
PsuI RGATCY 2 cut(s) 520, 938
PvuII CAGCTG 2 cut(s) 362, 1594
RsaI GTAC 5 cut(s) 267, 945, 1569, 2025, 2098
RsaNI GTAC 5 cut(s) 266, 944, 1568, 2024, 2097
RseI CAYNNNNRTG 2 cut(s) 311, 1457
SaqAI TTAA 7 cut(s) 303, 509, 675, 773, 857, 959, 1094
SatI GCNGC 7 cut(s) 360, 1205, 2084, 2303, 2318, 2321, 2388
Sau3AI GATC 7 cut(s) 520, 938, 1376, 1432, 2177, 2469, 2505
Sau96I GGNCC 6 cut(s) 1279, 1608, 1695, 1696, 1984, 2463
SchI GAGTC 6 cut(s) 424, 829, 1726, 1762, 2087, 2228
ScrFI CCNGG 2 cut(s) 525, 1629
SduI GDGCHC 3 cut(s) 417, 1478, 1699
SfaNI GCATC 8 cut(s) 961, 1186, 1680, 2287, 2304, 2364, 2386, 2522
SfcI CTRYAG 1 cut(s) 2249
SfuI TTCGAA 1 cut(s) 2044
SinI GGWCC 4 cut(s) 1279, 1608, 1984, 2463
SmiMI CAYNNNNRTG 2 cut(s) 311, 1457
SmlI CTYRAG 6 cut(s) 110, 383, 508, 1759, 2092, 2282
SmoI CTYRAG 6 cut(s) 110, 383, 508, 1759, 2092, 2282
SpeI ACTAGT 1 cut(s) 2329
SphI GCATGC 3 cut(s) 1087, 1112, 1795
SseBI AGGCCT 1 cut(s) 2111
SsiI CCGC 3 cut(s) 1205, 1543, 1693
SspI AATATT 1 cut(s) 250
StuI AGGCCT 1 cut(s) 2111
StyD4I CCNGG 2 cut(s) 523, 1627
TaaI ACNGT 4 cut(s) 722, 1639, 2066, 2417
TaiI ACGT 5 cut(s) 183, 347, 420, 543, 951
TaqII GACCGA 1 cut(s) 158
TatI WGTACW 2 cut(s) 943, 2096
TauI GCSGC 1 cut(s) 1207
TfiI GAWTC 9 cut(s) 130, 324, 917, 1400, 1680, 1783, 1808, 1861, 2006
Tru1I TTAA 7 cut(s) 303, 509, 675, 773, 857, 959, 1094
Tru9I TTAA 7 cut(s) 303, 509, 675, 773, 857, 959, 1094
TseFI GTSAC 5 cut(s) 13, 37, 88, 901, 1754
TseI GCWGC 6 cut(s) 359, 2083, 2302, 2317, 2320, 2387
Tsp45I GTSAC 5 cut(s) 13, 37, 88, 901, 1754
TspDTI ATGAA 8 cut(s) 213, 485, 747, 930, 1397, 1402, 1430, 1574
Vha464I CTTAAG 1 cut(s) 508
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 4 cut(s) 1279, 1608, 1984, 2463
XapI RAATTY 6 cut(s) 200, 1389, 1486, 1828, 2046, 2203
XbaI TCTAGA 3 cut(s) 1373, 1768, 2074
XceI RCATGY 5 cut(s) 1087, 1112, 1532, 1671, 1795
XcmI CCANNNNNNNNNTGG 1 cut(s) 1930
XmiI GTMKAC 1 cut(s) 2248
XmnI GAANNNNTTC 2 cut(s) 1488, 2163
ZraI GACGTC 1 cut(s) 181
Zsp2I ATGCAT 4 cut(s) 1201, 1431, 1673, 2379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.