Rh4DG089700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000159
Physical Location & Seq
Reverse (-)
13579623 .. 13583298
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG089700.1

Sequence Viewer

Length: 2592 bp
ATGGAGTTTGCCACCGGCATTGCTGAGGACGTCTTGGGCAGGCTAGCCTCCTATGCTGCCCAAGAAGTTTGCTTGGCATGGGGTGCCCAACTTGAGCTGAAGAAGCTCAGTAAGACCTTGGTACCCATCAGACTTGTGCTCCAAGATGCAGAGAAGAAGCAAATGCAAAATCCCCTCATTACTCATTGGTTAGAAAACCTCAAAGATGTTTGCAAAGATGCTGAGGATGTACTCGACGAATTTGAGTTCCGAAAGCTCCGACTCAAAGTGCTCACCAACAACCGGCAAAGTGTCAAAGGAAAGGTACACCAATTCTTCTCTCATTGGAATCCAGTCGTGTTTAACTTCAAAATGGGTCATAAAATTAAAGAGATTAGAGAAAGACTAGTTGAGATTGATGATGAAAAGACAAAATTTTCTCTCATTCAAATTGCTGAACAAGCTGAAGACTATCGTGTGCCCAAAACGGTGCTAAGGGAGACCGACTCTTTACCGGAGGCGTATGTTGTTGGAAGAGATGAGGACAAAAAACAGATTATAACCCATCTCTTGAGTGACATCGATTCCTCCAGTAATGAAAATGTTTCGGTTATCTCCATCATCGGGTTAGGTGGGTTGGGAAAAACTACACTTGCTAAAATGGTGTACAATGATAATGTGGTAGAAGAAAACTTCGAGACGAGATTGTGGATATGTGTCTCAGATAAATTTGATATCTGTACATTAGTTAAAGGCATTATTGCTGAAACTAAAAAAGAATGTGATTGTGGAAGTGTGAATCTTATGAAAAGGGGGTTGCAACATATCTTGAGAGGTACAAAATTTTTGTTAGTGTTGGATGATGTTTGGGAGACTGAGTCAACAGGATTTACAGTTCAAAAATGGGCCGAGTTAAAAACTGTGTTAGATGTTGGAGTCAAAGGGAGTAAAATCATTGTAACAACCCGCAATACATCAGTCGCTTCACTTGTGCACCCTATACATATGCATTTTTTAGAAGGTCTTTCTCATGAGGATTCCATAACTTTGTTTAAAAAAAATGCATTTAACGAAGGAGATGAGCAATGTTATCAGAAATTGCTAGAAATTGGGGAGGACATTGTCAAGAAGTGTAGAGGGGTTCCCTTAGCACTAGCTACTCTAGGGAGTTTGCTCCGCTCGAAAAGGGACCGACGCCACTGGATACAAGTACGAGATGATGAAATATGGAGTATGTCAGGAAACGACAATATTATGGCTGCACTAAAATTGAGTTACAATGCATTGTCACCTCACTTGAAACCTTGTTTTGCTTTTTGTTCACTTTACCCCAAGGATTATAGATTTGAGAGTGAAGAAATAGTCTCATTATGGATGGCACAAGGCTTCCTCAAGTCCTCGAGAGAAAATGAAGATTTTGAGGAGATGGGCCTAGACTATATAAGGCAGTTATGTTCCAAGTCTTTGTTTCAAATTGAGTCCGATAGCAAGACTACCCTGGGGTTCAAAATACATGATTTAGTGCACGATTTAGCAATATCAGTGGCAGGAATAGAGTGCTCCACAGTTAATTTTCGTCCCTCTAGTGCTTTTGAAAAAGTCCGGCACATGTCAATGACAGCCAAAGACTTGTTCGGAGAAGAGGCACAAGTCCCAGAATTCATTCTCGCGTTGGAGAAACTACGGACCATTCTAAACCTGGATAGTGATGCTAGGATCTCCAGTCAATATTTTCTGAGGACATGCATCTCGAGATTCAAGTATATGCGTGCACTAGATGTACGTGGTTCAACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGAGTCATTTGAGATATCTCAACCTGGATTTTAATAGGAAGATAAAAAAGCTTCCCACTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTCGAAGGTTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAACCTCAGATTTTTGAATATAACTACAAAGCAGATGTATTTTCCGAAAGGAATCTTTAGGCGCCTCACGTCACTTCGAATTATATACATTGGGTATTGTAAGAATCTAAAATCTCTGGGGGAAGAGATTGGATACCTCACTAACCTTCGTCAACTGTGGATTGATGACTGTGGAAATCTGGAGTCCTTGCCATCTAACATGGAGAACTTATCTGCATTTCATACTTTGTTCATCTTTGATTGTAAGAAGCTTGAGTTAATGCAGTCGGGACAAGGCATCAAAGGTCTTCGAACATTCTTTATCAGAGGTAGTTCAGATTTGGAGGCTTTGCCCAATTGGCTTCAAGATTCTGCAGACACTTTACAGAGCATAGGAATCACGTCGTGTGATAATTTCACTGCACTTCCAGAGTGGTTGCCAAAACTGACCTCGTTGGAGCAACTAGTGATTAGGACCTGCCCCAGATTGTCTGCTTTGCCGCAGGGGATGCATTGTCTGACTGCGATGAGAGAACTGGAGTTCAAACGGTGTCCTGAATTGAGAAAAAGATGCAAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAACATGTCCCAAAAATTGATATTGATGACTCTGATGATGATCAGATGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

863

Amino Acids

98.64

Weight (kDa)

6.39

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 96 2.1e-20 Rx N-terminal domain
NB-ARC PF00931 177 - 351 9.1e-33 NB-ARC domain
WHD_DRP PF23559 435 - 505 1.5e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 773 2e-27 Leucine-rich repeat region
LRR_13 PF23286 590 - 680 3.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 2417
Acc65I GGTACC 1 cut(s) 121
AccB1I GGYRCC 3 cut(s) 83, 121, 2013
AccBSI CCGCTC 1 cut(s) 1158
AccII CGCG 1 cut(s) 1649
AciI CCGC 3 cut(s) 946, 1156, 2432
AclWI GGATC 1 cut(s) 1703
AcsI RAATTY 6 cut(s) 239, 413, 707, 821, 1637, 1882
AcuI CTGAAG 2 cut(s) 119, 465
AcyI GRCGYC 3 cut(s) 30, 1174, 2014
AdeI CACNNNGTG 1 cut(s) 2337
AfaI GTAC 8 cut(s) 123, 231, 306, 647, 721, 817, 1191, 1761
AfiI CCNNNNNNNGG 2 cut(s) 282, 603
AflII CTTAAG 1 cut(s) 1894
AflIII ACRYGT 2 cut(s) 1587, 2542
AhlI ACTAGT 2 cut(s) 385, 2395
AjiI CACGTC 2 cut(s) 2022, 2334
AjnI CCWGG 3 cut(s) 1476, 1677, 1827
AjuI GAANNNNNNNTTGG 2 cut(s) 1595, 1627
AloI GAACNNNNNNTCC 2 cut(s) 858, 890
AluBI AGCT 9 cut(s) 97, 106, 256, 443, 1136, 1783, 1855, 1876, 2203
AluI AGCT 9 cut(s) 97, 106, 256, 443, 1136, 1783, 1855, 1876, 2203
Alw21I GWGCWC 6 cut(s) 141, 273, 975, 1506, 1541, 1753
Alw26I GTCTC 5 cut(s) 473, 671, 703, 845, 1348
Alw44I GTGCAC 3 cut(s) 971, 1502, 1749
AlwI GGATC 1 cut(s) 1703
Ama87I CYCGRG 2 cut(s) 1378, 1729
AoxI GGCC 2 cut(s) 885, 1408
ApaLI GTGCAC 3 cut(s) 971, 1502, 1749
ApeKI GCWGC 2 cut(s) 56, 1238
ApoI RAATTY 6 cut(s) 239, 413, 707, 821, 1637, 1882
Asp700I GAANNNNTTC 1 cut(s) 1641
Asp718I GGTACC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 2016
AspS9I GGNCC 6 cut(s) 885, 1168, 1408, 1665, 2406, 2529
AsuHPI GGTGA 2 cut(s) 265, 1260
AsuII TTCGAA 2 cut(s) 2029, 2242
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 1378, 1729
AvaII GGWCC 4 cut(s) 1168, 1665, 2406, 2529
BaeGI GKGCMC 5 cut(s) 88, 462, 975, 1506, 1753
BaeI ACNNNNGTAYC 2 cut(s) 105, 138
BanI GGYRCC 3 cut(s) 83, 121, 2013
BbsI GAAGAC 2 cut(s) 453, 2231
Bbv12I GWGCWC 6 cut(s) 141, 273, 975, 1506, 1541, 1753
BbvCI CCTCAGC 2 cut(s) 24, 222
BbvI GCAGC 2 cut(s) 43, 1225
BccI CCATC 6 cut(s) 134, 552, 605, 1348, 1399, 2152
BciT130I CCWGG 3 cut(s) 1478, 1679, 1829
BciVI GTATCC 2 cut(s) 1176, 2078
BclI TGATCA 2 cut(s) 1950, 2578
BcoDI GTCTC 5 cut(s) 473, 671, 703, 845, 1348
BcuI ACTAGT 2 cut(s) 385, 2395
BfmI CTRYAG 1 cut(s) 2304
BfoI RGCGCY 1 cut(s) 2017
BfrI CTTAAG 1 cut(s) 1894
BfuAI ACCTGC 1 cut(s) 2417
BfuI GTATCC 2 cut(s) 1176, 2078
BglI GCCNNNNNGGC 1 cut(s) 2290
BisI GCNGC 3 cut(s) 57, 1239, 2432
BlsI GCNGC 3 cut(s) 58, 1240, 2433
Bme1390I CCNGG 3 cut(s) 1478, 1679, 1829
Bme18I GGWCC 4 cut(s) 1168, 1665, 2406, 2529
BmeT110I CYCGRG 2 cut(s) 1378, 1729
BmgBI CACGTC 2 cut(s) 2022, 2334
BmgT120I GGNCC 6 cut(s) 885, 1168, 1408, 1665, 2406, 2529
BmiI GGNNCC 6 cut(s) 85, 123, 1122, 1169, 1946, 2015
BmrFI CCNGG 3 cut(s) 1478, 1679, 1829
BmsI GCATC 7 cut(s) 136, 208, 1678, 1734, 2238, 2430, 2492
BmtI GCTAGC 1 cut(s) 47
BpiI GAAGAC 2 cut(s) 453, 2231
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BpmI CTGGAG 4 cut(s) 553, 1684, 2153, 2489
Bpu10I CCTNAGC 4 cut(s) 24, 222, 473, 1126
Bpu14I TTCGAA 2 cut(s) 2029, 2242
BpuEI CTTGAG 8 cut(s) 113, 571, 829, 1355, 1796, 1825, 1940, 2225
Bsa29I ATCGAT 1 cut(s) 561
BsaAI YACGTR 1 cut(s) 1763
BsaBI GATNNNNATC 1 cut(s) 2577
BsaHI GRCGYC 3 cut(s) 30, 1174, 2014
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 4 cut(s) 117, 1311, 1476, 1477
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 4 cut(s) 123, 153, 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 603
Bse118I RCCGGY 2 cut(s) 14, 282
Bse1I ACTGG 5 cut(s) 332, 570, 1184, 1701, 2472
Bse3DI GCAATG 2 cut(s) 18, 1070
Bse8I GATNNNNATC 1 cut(s) 2577
BseBI CCWGG 3 cut(s) 1478, 1679, 1829
BseCI ATCGAT 1 cut(s) 561
BseDI CCNNGG 4 cut(s) 117, 1311, 1476, 1477
BseGI GGATG 4 cut(s) 232, 844, 1359, 2445
BseJI GATNNNNATC 1 cut(s) 2577
BseLI CCNNNNNNNGG 2 cut(s) 282, 603
BseMI GCAATG 2 cut(s) 18, 1070
BseMII CTCAG 7 cut(s) 15, 121, 213, 714, 846, 1706, 1972
BseNI ACTGG 5 cut(s) 332, 570, 1184, 1701, 2472
BseRI GAGGAG 3 cut(s) 1415, 1793, 1937
BseSI GKGCMC 5 cut(s) 88, 462, 975, 1506, 1753
BseXI GCAGC 2 cut(s) 43, 1225
BsgI GTGCAG 2 cut(s) 1224, 2337
Bsh1236I CGCG 1 cut(s) 1649
BshFI GGCC 2 cut(s) 887, 1410
BshNI GGYRCC 3 cut(s) 83, 121, 2013
BshVI ATCGAT 1 cut(s) 561
BsiHKAI GWGCWC 6 cut(s) 141, 273, 975, 1506, 1541, 1753
BsiHKCI CYCGRG 2 cut(s) 1378, 1729
BsiSI CCGG 4 cut(s) 15, 283, 494, 1582
BslFI GGGAC 5 cut(s) 1181, 1542, 1616, 2235, 2531
BslI CCNNNNNNNGG 2 cut(s) 282, 603
BsmAI GTCTC 5 cut(s) 473, 671, 703, 845, 1348
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 5 cut(s) 1181, 1542, 1616, 2235, 2531
BsnI GGCC 2 cut(s) 887, 1410
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 2 cut(s) 1378, 1729
Bsp119I TTCGAA 2 cut(s) 2029, 2242
Bsp1286I GDGCHC 8 cut(s) 88, 141, 273, 462, 975, 1506, 1541, 1753
Bsp1407I TGTACA 2 cut(s) 645, 719
Bsp143I GATC 4 cut(s) 1695, 1950, 2535, 2578
BspACI CCGC 3 cut(s) 946, 1156, 2432
BspANI GGCC 2 cut(s) 887, 1410
BspCNI CTCAG 7 cut(s) 16, 120, 214, 713, 847, 1707, 1971
BspDI ATCGAT 1 cut(s) 561
BspFNI CGCG 1 cut(s) 1649
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 6 cut(s) 85, 123, 1122, 1169, 1946, 2015
BspMAI CTGCAG 1 cut(s) 2308
BspMI ACCTGC 1 cut(s) 2417
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 1703
BspT104I TTCGAA 2 cut(s) 2029, 2242
BspT107I GGYRCC 3 cut(s) 83, 121, 2013
BspTI CTTAAG 1 cut(s) 1894
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 1158
BsrDI GCAATG 2 cut(s) 18, 1070
BsrFI RCCGGY 2 cut(s) 14, 282
BsrGI TGTACA 2 cut(s) 645, 719
BsrI ACTGG 5 cut(s) 332, 570, 1184, 1701, 2472
BssAI RCCGGY 2 cut(s) 14, 282
BssECI CCNNGG 4 cut(s) 117, 1311, 1476, 1477
BssMI GATC 4 cut(s) 1695, 1950, 2535, 2578
BssNI GRCGYC 3 cut(s) 30, 1174, 2014
BssT1I CCWWGG 2 cut(s) 117, 1311
Bst2UI CCWGG 3 cut(s) 1478, 1679, 1829
Bst4CI ACNGT 7 cut(s) 469, 874, 901, 1546, 2109, 2123, 2481
Bst6I CTCTTC 3 cut(s) 508, 1614, 2070
BstACI GRCGYC 3 cut(s) 30, 1174, 2014
BstAFI CTTAAG 1 cut(s) 1894
BstAPI GCANNNNNTGC 2 cut(s) 83, 2440
BstAUI TGTACA 2 cut(s) 645, 719
BstBAI YACGTR 1 cut(s) 1763
BstBI TTCGAA 2 cut(s) 2029, 2242
BstC8I GCNNGC 4 cut(s) 41, 45, 1749, 1874
BstDEI CTNAG 9 cut(s) 24, 107, 222, 473, 700, 855, 1126, 1715, 1958
BstF5I GGATG 4 cut(s) 232, 844, 1359, 2445
BstFNI CGCG 1 cut(s) 1649
BstH2I RGCGCY 1 cut(s) 2017
BstHHI GCGC 1 cut(s) 2016
BstKTI GATC 4 cut(s) 1698, 1953, 2538, 2581
BstMAI GTCTC 5 cut(s) 473, 671, 703, 845, 1348
BstMBI GATC 4 cut(s) 1695, 1950, 2535, 2578
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 440, 2290, 2440
BstNI CCWGG 3 cut(s) 1478, 1679, 1829
BstNSI RCATGY 3 cut(s) 1591, 1725, 2546
BstSCI CCNGG 3 cut(s) 1476, 1677, 1827
BstSFI CTRYAG 1 cut(s) 2304
BstSLI GKGCMC 5 cut(s) 88, 462, 975, 1506, 1753
BstUI CGCG 1 cut(s) 1649
BstV1I GCAGC 2 cut(s) 43, 1225
BstV2I GAAGAC 2 cut(s) 453, 2231
BstX2I RGATCY 1 cut(s) 1695
BstYI RGATCY 1 cut(s) 1695
Bsu15I ATCGAT 1 cut(s) 561
BsuI GTATCC 2 cut(s) 1176, 2078
BsuRI GGCC 2 cut(s) 887, 1410
BsuTUI ATCGAT 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 2471
BtrI CACGTC 2 cut(s) 2022, 2334
BtsCI GGATG 4 cut(s) 232, 844, 1359, 2445
BtsI GCAGTG 1 cut(s) 2349
BtsIMutI CAGTG 3 cut(s) 1177, 1527, 2349
BveI ACCTGC 1 cut(s) 2417
Cac8I GCNNGC 4 cut(s) 41, 45, 1749, 1874
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 1 cut(s) 2016
Cfr10I RCCGGY 2 cut(s) 14, 282
Cfr13I GGNCC 6 cut(s) 885, 1168, 1408, 1665, 2406, 2529
ClaI ATCGAT 1 cut(s) 561
CseI GACGC 1 cut(s) 1182
Csp6I GTAC 8 cut(s) 122, 230, 305, 646, 720, 816, 1190, 1760
CspCI CAANNNNNGTGG 3 cut(s) 36, 1503, 1538
CviAII CATG 7 cut(s) 78, 1010, 1493, 1588, 1722, 2152, 2543
CviQI GTAC 8 cut(s) 122, 230, 305, 646, 720, 816, 1190, 1760
DdeI CTNAG 9 cut(s) 24, 107, 222, 473, 700, 855, 1126, 1715, 1958
DinI GGCGCC 1 cut(s) 2015
DpnI GATC 4 cut(s) 1697, 1952, 2537, 2580
DpnII GATC 4 cut(s) 1695, 1950, 2535, 2578
DraI TTTAAA 1 cut(s) 1033
DraIII CACNNNGTG 1 cut(s) 2337
Eam1104I CTCTTC 3 cut(s) 508, 1614, 2070
EarI CTCTTC 3 cut(s) 508, 1614, 2070
Eco130I CCWWGG 2 cut(s) 117, 1311
Eco31I GGTCTC 1 cut(s) 473
Eco32I GATATC 2 cut(s) 715, 1820
Eco47I GGWCC 4 cut(s) 1168, 1665, 2406, 2529
Eco57I CTGAAG 2 cut(s) 119, 465
Eco88I CYCGRG 2 cut(s) 1378, 1729
EcoO109I RGGNCCY 1 cut(s) 2406
EcoRI GAATTC 1 cut(s) 1637
EcoRII CCWGG 3 cut(s) 1476, 1677, 1827
EcoRV GATATC 2 cut(s) 715, 1820
EcoT14I CCWWGG 2 cut(s) 117, 1311
EcoT22I ATGCAT 5 cut(s) 990, 1045, 1264, 1727, 2445
EgeI GGCGCC 1 cut(s) 2015
EheI GGCGCC 1 cut(s) 2015
ErhI CCWWGG 2 cut(s) 117, 1311
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 7 cut(s) 81, 1013, 1496, 1591, 1725, 2155, 2546
FaqI GGGAC 5 cut(s) 1181, 1542, 1616, 2235, 2531
FatI CATG 7 cut(s) 77, 1009, 1492, 1587, 1721, 2151, 2542
FauI CCCGC 1 cut(s) 953
FauNDI CATATG 1 cut(s) 984
FbaI TGATCA 2 cut(s) 1950, 2578
Fnu4HI GCNGC 3 cut(s) 57, 1239, 2432
FokI GGATG 4 cut(s) 239, 851, 1366, 2452
Fsp4HI GCNGC 3 cut(s) 57, 1239, 2432
GlaI GCGC 1 cut(s) 2015
GluI GCNGC 3 cut(s) 57, 1239, 2432
GsuI CTGGAG 4 cut(s) 553, 1684, 2153, 2489
HaeII RGCGCY 1 cut(s) 2017
HaeIII GGCC 2 cut(s) 887, 1410
HapII CCGG 4 cut(s) 15, 283, 494, 1582
HgaI GACGC 1 cut(s) 1182
HhaI GCGC 1 cut(s) 2016
Hin1I GRCGYC 3 cut(s) 30, 1174, 2014
Hin1II CATG 7 cut(s) 81, 1013, 1496, 1591, 1725, 2155, 2546
Hin6I GCGC 1 cut(s) 2014
HinP1I GCGC 1 cut(s) 2014
HincII GTYRAC 2 cut(s) 861, 2105
HindII GTYRAC 2 cut(s) 861, 2105
HindIII AAGCTT 2 cut(s) 1853, 2201
HpaII CCGG 4 cut(s) 15, 283, 494, 1582
HphI GGTGA 2 cut(s) 265, 1260
Hpy166II GTNNAC 8 cut(s) 307, 646, 861, 973, 1301, 1504, 1751, 2105
Hpy8I GTNNAC 8 cut(s) 307, 646, 861, 973, 1301, 1504, 1751, 2105
Hpy99I CGWCG 3 cut(s) 239, 1176, 2338
HpyAV CCTTC 4 cut(s) 992, 1046, 1898, 2108
HpyCH4III ACNGT 7 cut(s) 469, 874, 901, 1546, 2109, 2123, 2481
HpyCH4IV ACGT 4 cut(s) 30, 1762, 2021, 2333
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 440, 2290, 2440
HpyF3I CTNAG 9 cut(s) 24, 107, 222, 473, 700, 855, 1126, 1715, 1958
HpySE526I ACGT 4 cut(s) 30, 1762, 2021, 2333
Hsp92I GRCGYC 3 cut(s) 30, 1174, 2014
Hsp92II CATG 7 cut(s) 81, 1013, 1496, 1591, 1725, 2155, 2546
HspAI GCGC 1 cut(s) 2014
KasI GGCGCC 1 cut(s) 2013
KpnI GGTACC 1 cut(s) 125
Ksp22I TGATCA 2 cut(s) 1950, 2578
Kzo9I GATC 4 cut(s) 1695, 1950, 2535, 2578
LmnI GCTCC 6 cut(s) 144, 261, 1158, 1544, 1780, 2389
Lsp1109I GCAGC 2 cut(s) 43, 1225
LweI GCATC 7 cut(s) 136, 208, 1678, 1734, 2238, 2430, 2492
MaeII ACGT 4 cut(s) 30, 1762, 2021, 2333
MaeIII GTNAC 7 cut(s) 554, 937, 1253, 1266, 1799, 1926, 2022
MalI GATC 4 cut(s) 1697, 1952, 2537, 2580
MbiI CCGCTC 1 cut(s) 1158
MboI GATC 4 cut(s) 1695, 1950, 2535, 2578
MfeI CAATTG 1 cut(s) 2287
MflI RGATCY 1 cut(s) 1695
MhlI GDGCHC 8 cut(s) 88, 141, 273, 462, 975, 1506, 1541, 1753
Mly113I GGCGCC 1 cut(s) 2014
MlyI GAGTC 8 cut(s) 255, 479, 866, 924, 1466, 1816, 2144, 2561
MmeI TCCRAC 6 cut(s) 283, 490, 816, 892, 1632, 2367
Mph1103I ATGCAT 5 cut(s) 990, 1045, 1264, 1727, 2445
MroXI GAANNNNTTC 1 cut(s) 1641
MslI CAYNNNNRTG 1 cut(s) 1592
MspCI CTTAAG 1 cut(s) 1894
MspI CCGG 4 cut(s) 15, 283, 494, 1582
MspR9I CCNGG 3 cut(s) 1478, 1679, 1829
MunI CAATTG 1 cut(s) 2287
MvaI CCWGG 3 cut(s) 1478, 1679, 1829
MvnI CGCG 1 cut(s) 1649
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 440, 2290, 2440
NarI GGCGCC 1 cut(s) 2014
NdeI CATATG 1 cut(s) 984
NdeII GATC 4 cut(s) 1695, 1950, 2535, 2578
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 7 cut(s) 81, 1013, 1496, 1591, 1725, 2155, 2546
NlaIV GGNNCC 6 cut(s) 85, 123, 1122, 1169, 1946, 2015
NmeAIII GCCGAG 1 cut(s) 913
NmuCI GTSAC 3 cut(s) 554, 1266, 2022
NsiI ATGCAT 5 cut(s) 990, 1045, 1264, 1727, 2445
NspI RCATGY 3 cut(s) 1591, 1725, 2546
NspV TTCGAA 2 cut(s) 2029, 2242
PaeR7I CTCGAG 2 cut(s) 1378, 1729
PagI TCATGA 1 cut(s) 1009
PasI CCCWGGG 1 cut(s) 1477
PciI ACATGT 2 cut(s) 1587, 2542
PdmI GAANNNNTTC 1 cut(s) 1641
PfeI GAWTC 9 cut(s) 328, 563, 778, 1016, 1734, 2004, 2056, 2300, 2328
PflFI GACNNNGTC 2 cut(s) 856, 1100
PkrI GCNGC 3 cut(s) 58, 1240, 2433
PleI GAGTC 8 cut(s) 255, 479, 865, 923, 1465, 1815, 2143, 2561
PluTI GGCGCC 1 cut(s) 2017
PpsI GAGTC 8 cut(s) 255, 479, 865, 923, 1465, 1815, 2143, 2561
Ppu21I YACGTR 1 cut(s) 1763
PpuMI RGGWCCY 1 cut(s) 2406
PscI ACATGT 2 cut(s) 1587, 2542
PsiI TTATAA 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 2406
Psp6I CCWGG 3 cut(s) 1476, 1677, 1827
PspGI CCWGG 3 cut(s) 1476, 1677, 1827
PspN4I GGNNCC 6 cut(s) 85, 123, 1122, 1169, 1946, 2015
PspPI GGNCC 6 cut(s) 885, 1168, 1408, 1665, 2406, 2529
PspPPI RGGWCCY 1 cut(s) 2406
PstI CTGCAG 1 cut(s) 2308
PsuI RGATCY 1 cut(s) 1695
PsyI GACNNNGTC 2 cut(s) 856, 1100
RsaI GTAC 8 cut(s) 123, 231, 306, 647, 721, 817, 1191, 1761
RsaNI GTAC 8 cut(s) 122, 230, 305, 646, 720, 816, 1190, 1760
RseI CAYNNNNRTG 1 cut(s) 1592
SatI GCNGC 3 cut(s) 57, 1239, 2432
Sau3AI GATC 4 cut(s) 1695, 1950, 2535, 2578
Sau96I GGNCC 6 cut(s) 885, 1168, 1408, 1665, 2406, 2529
SchI GAGTC 8 cut(s) 255, 479, 866, 924, 1466, 1816, 2144, 2561
ScrFI CCNGG 3 cut(s) 1478, 1679, 1829
SduI GDGCHC 8 cut(s) 88, 141, 273, 462, 975, 1506, 1541, 1753
SfaNI GCATC 7 cut(s) 136, 208, 1678, 1734, 2238, 2430, 2492
SfcI CTRYAG 1 cut(s) 2304
SfoI GGCGCC 1 cut(s) 2015
Sfr274I CTCGAG 2 cut(s) 1378, 1729
SfuI TTCGAA 2 cut(s) 2029, 2242
SinI GGWCC 4 cut(s) 1168, 1665, 2406, 2529
SlaI CTCGAG 2 cut(s) 1378, 1729
SmiMI CAYNNNNRTG 1 cut(s) 1592
SpeI ACTAGT 2 cut(s) 385, 2395
SsiI CCGC 3 cut(s) 946, 1156, 2432
SspDI GGCGCC 1 cut(s) 2013
SspI AATATT 2 cut(s) 1231, 1709
StyD4I CCNGG 3 cut(s) 1476, 1677, 1827
StyI CCWWGG 2 cut(s) 117, 1311
TaaI ACNGT 7 cut(s) 469, 874, 901, 1546, 2109, 2123, 2481
TaiI ACGT 4 cut(s) 33, 1765, 2024, 2336
TaqI TCGA 9 cut(s) 234, 561, 675, 1160, 1379, 1730, 1902, 2029, 2242
TaqII GACCGA 2 cut(s) 497, 1185
TatI WGTACW 3 cut(s) 229, 645, 719
TauI GCSGC 1 cut(s) 2434
TfiI GAWTC 9 cut(s) 328, 563, 778, 1016, 1734, 2004, 2056, 2300, 2328
TscAI CASTG 3 cut(s) 1184, 1527, 2356
TseFI GTSAC 3 cut(s) 554, 1266, 2022
TseI GCWGC 2 cut(s) 56, 1238
Tsp45I GTSAC 3 cut(s) 554, 1266, 2022
TspDTI ATGAA 8 cut(s) 417, 591, 800, 1215, 1404, 1630, 2162, 2173
TspGWI ACGGA 1 cut(s) 1678
TspRI CASTG 3 cut(s) 1184, 1527, 2356
Tth111I GACNNNGTC 2 cut(s) 856, 1100
Vha464I CTTAAG 1 cut(s) 1894
VneI GTGCAC 3 cut(s) 971, 1502, 1749
VpaK11BI GGWCC 4 cut(s) 1168, 1665, 2406, 2529
XapI RAATTY 6 cut(s) 239, 413, 707, 821, 1637, 1882
XceI RCATGY 3 cut(s) 1591, 1725, 2546
XcmI CCANNNNNNNNNTGG 1 cut(s) 1675
XhoI CTCGAG 2 cut(s) 1378, 1729
XmnI GAANNNNTTC 1 cut(s) 1641
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 5 cut(s) 990, 1045, 1264, 1727, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.