Rh2AG263600

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000159
Physical Location & Seq
Forward (+)
30422735 .. 30424190
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG263600.1

Sequence Viewer

Length: 576 bp
ATGGTTCGACATGTTTCAATATCTGAAAAGGACTTGCTTGGAGAAGAGGCACAAGTTCTCGATTTCATGCTGAAGTCAAAGAAATTGCGAACTATTCTAGTTCCTCAAGATGGTCAAATGAGTCAACGTTTTGTGAAGACATTCATCTCGAGATTCAAATTTATGCGGGTGCTAGATCTCAGTTTGTCGCCTCAGGAGGAGCTACCAAGTTCCGTTGGTAGTTTATTTCATTTGAGATTTCTCAACTTGTCTGAAAATTGGAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGACCTTGTGCCTTGCGGGTTGCGACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTCCGAACTTTGGTCAGAGGAAGAAGAGGGTCACTAGCTACAAGGTGTACACTGTGGAGGGAAGCTGAAAGGGACTCTAAGGAAGAGCCAATATGGACTGCTTTTAAGGGGGAGATGGAGGTGTCAGAGAGGCTGGAGGGTGCAAAAGGCGTTGGAGAGTGCTGTGGACGATATGGACTTGATGGGGAGGCTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

8.28

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 47 - 133 6.1e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 166, 323
AclI AACGTT 1 cut(s) 127
AcsI RAATTY 2 cut(s) 158, 301
AcuI CTGAAG 1 cut(s) 92
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 2 cut(s) 110, 385
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 18, 157
AluBI AGCT 4 cut(s) 202, 295, 413, 440
AluI AGCT 4 cut(s) 202, 295, 413, 440
Alw26I GTCTC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 2 cut(s) 158, 301
Asp700I GAANNNNTTC 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 282
BccI CCATC 4 cut(s) 104, 293, 484, 551
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 3 cut(s) 98, 173, 410
BglII AGATCT 1 cut(s) 175
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 90, 359
BsaI GGTCTC 1 cut(s) 302
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 385
Bse21I CCTNAGG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 110, 385
BseMII CTCAG 2 cut(s) 193, 206
BseRI GAGGAG 2 cut(s) 212, 356
BseXI GCAGC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 2 cut(s) 110, 385
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 461
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 422
Bsp143I GATC 2 cut(s) 175, 369
BspACI CCGC 2 cut(s) 166, 323
BspCNI CTCAG 2 cut(s) 192, 205
BspLI GGNNCC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 422
BssMI GATC 2 cut(s) 175, 369
Bst4CI ACNGT 1 cut(s) 429
Bst6I CTCTTC 3 cut(s) 39, 394, 453
BstAUI TGTACA 1 cut(s) 422
BstDEI CTNAG 3 cut(s) 179, 192, 453
BstKTI GATC 2 cut(s) 178, 372
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 2 cut(s) 175, 369
BstNSI RCATGY 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 425
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 423
CviAII CATG 2 cut(s) 11, 67
CviJI RGCY 8 cut(s) 202, 274, 295, 413, 440, 463, 508, 566
CviKI_1 RGCY 8 cut(s) 202, 274, 295, 413, 440, 463, 508, 566
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 3 cut(s) 179, 192, 453
DpnI GATC 2 cut(s) 177, 371
DpnII GATC 2 cut(s) 175, 369
Eam1104I CTCTTC 3 cut(s) 39, 394, 453
EarI CTCTTC 3 cut(s) 39, 394, 453
Eco31I GGTCTC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 148
FaeI CATG 2 cut(s) 14, 70
FaiI YATR 6 cut(s) 12, 68, 164, 359, 469, 549
FaqI GGGAC 1 cut(s) 461
FatI CATG 2 cut(s) 10, 66
FauI CCCGC 2 cut(s) 159, 316
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 3 cut(s) 98, 173, 410
GluI GCNGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 530
HgaI GACGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 14, 70
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HinfI GANTC 3 cut(s) 121, 153, 449
Hpy166II GTNNAC 4 cut(s) 125, 423, 425, 542
Hpy188I TCNGA 5 cut(s) 25, 253, 380, 392, 502
Hpy188III TCNNGA 5 cut(s) 59, 107, 148, 150, 194
Hpy8I GTNNAC 4 cut(s) 125, 423, 425, 542
Hpy99I CGWCG 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 518
HpyF3I CTNAG 3 cut(s) 179, 192, 453
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 2 cut(s) 14, 70
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 2 cut(s) 175, 369
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 3 cut(s) 179, 380, 494
Lsp1109I GCAGC 1 cut(s) 282
MaeI CTAG 3 cut(s) 98, 173, 410
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 405
MalI GATC 2 cut(s) 177, 371
MboI GATC 2 cut(s) 175, 369
MboII GAAGA 6 cut(s) 56, 148, 274, 408, 411, 470
MflI RGATCY 1 cut(s) 175
MluCI AATT 5 cut(s) 83, 158, 256, 280, 301
MlyI GAGTC 2 cut(s) 130, 443
MmeI TCCRAC 1 cut(s) 508
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 1 cut(s) 480
NdeII GATC 2 cut(s) 175, 369
NlaIII CATG 2 cut(s) 14, 70
NlaIV GGNNCC 1 cut(s) 365
NmuCI GTSAC 1 cut(s) 405
NspI RCATGY 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 148
PciI ACATGT 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 153
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 2 cut(s) 129, 443
PpsI GAGTC 2 cut(s) 129, 443
PscI ACATGT 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 365
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 2 cut(s) 175, 369
SchI GAGTC 2 cut(s) 130, 443
Sfr274I CTCGAG 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 3 cut(s) 105, 148, 338
SmoI CTYRAG 3 cut(s) 105, 148, 338
Sse9I AATT 5 cut(s) 83, 158, 256, 280, 301
SsiI CCGC 2 cut(s) 166, 323
SspMI CTAG 3 cut(s) 98, 173, 410
TaaI ACNGT 1 cut(s) 429
TaiI ACGT 1 cut(s) 130
TaqI TCGA 3 cut(s) 7, 60, 149
TasI AATT 5 cut(s) 83, 158, 256, 280, 301
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 3 cut(s) 55, 133, 218
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 1 cut(s) 432
XapI RAATTY 2 cut(s) 158, 301
XceI RCATGY 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 140
XspI CTAG 3 cut(s) 98, 173, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.