RLG00000018364

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000159
Physical Location & Seq
Reverse (-)
28825270 .. 28826088
819 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018364

Sequence Viewer

Length: 699 bp
ATGGTTCGACATGTTTCAATTTCCAAAAAAGACTTGCTTGGAGAAGAGGCACAAGTTCCCGATTTCATGCTCAAGTCAAAGAAATTGCGAACCATTCTAATTTTTGAAGATGGCAAGATGAAACAAAGTTTTGTGAAGACATGCATCTCAAGATTCAAATGTCTGCAGGTCCTAGATCTCAATGGGTCGCCTCTTGAGGAGCTACCGAGTTCCATTGGTAGTTTGTTTCATTTGAGATTTCTCAACTTGTATTCTAATAGTAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGACCTTGTCCTTGGTGGGTTCTGACGCACTTGAGGGATGTGTCAATCTTAAGTCTTTGGGCGAAGAGATCGAATTTCTCAACAACCTTCGCAAATTGTGGATTTGCAGGTGTAATAATTTGGAATCCTTGCCACCAAATATGAAACACATGATTGCTTTGGATACTTTGGCTATAGGTGATTGCGAAAAGCTTCAGGTGATGAGATCAGGGGATGGTGCTCAACGTCTTCGATCATTGATTATCGAGAAATCAAGTTTGGAGGCTTTGTCCCCTTGGCTTGAAGATTCTGCAGACACTCTACAGAGCATAGCCTTTTTAGAATGTCATAATCTCACGGCACTTCCGGAGTTTATGAACTTCACATTCCTCGAGCAACTACACATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

233

Amino Acids

26.29

Weight (kDa)

6.53

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 29 - 164 2.4e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 405
Acc36I ACCTGC 2 cut(s) 157, 405
AccIII TCCGGA 1 cut(s) 652
AcsI RAATTY 2 cut(s) 301, 380
AcuI CTGAAG 1 cut(s) 485
AflII CTTAAG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 4 cut(s) 18, 107, 157, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AluBI AGCT 3 cut(s) 202, 295, 499
AluI AGCT 3 cut(s) 202, 295, 499
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 677
Aor13HI TCCGGA 1 cut(s) 652
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 2 cut(s) 301, 380
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 2 cut(s) 497, 517
AvaI CYCGRG 1 cut(s) 677
AvaII GGWCC 1 cut(s) 169
BbsI GAAGAC 2 cut(s) 143, 527
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 282
BccI CCATC 3 cut(s) 104, 293, 515
BceAI ACGGC 1 cut(s) 660
BciVI GTATCC 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 4 cut(s) 164, 480, 597, 608
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 2 cut(s) 157, 405
BfuI GTATCC 1 cut(s) 463
BglII AGATCT 1 cut(s) 175
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 677
BmgT120I GGNCC 1 cut(s) 169
BmsI GCATC 1 cut(s) 153
BpiI GAAGAC 2 cut(s) 143, 527
BpuEI CTTGAG 4 cut(s) 56, 133, 215, 359
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 318, 581
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 560, 590
BseAI TCCGGA 1 cut(s) 652
BseDI CCNNGG 2 cut(s) 318, 581
BseGI GGATG 2 cut(s) 350, 526
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 1 cut(s) 653
BslFI GGGAC 1 cut(s) 562
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 562
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 677
Bsp1286I GDGCHC 1 cut(s) 529
Bsp13I TCCGGA 1 cut(s) 652
Bsp143I GATC 4 cut(s) 175, 375, 512, 539
BspEI TCCGGA 1 cut(s) 652
BspMAI CTGCAG 2 cut(s) 168, 601
BspMI ACCTGC 2 cut(s) 157, 405
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 302
BssECI CCNNGG 2 cut(s) 318, 581
BssMI GATC 4 cut(s) 175, 375, 512, 539
BssT1I CCWWGG 2 cut(s) 318, 581
Bst6I CTCTTC 2 cut(s) 39, 366
BstAFI CTTAAG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 350, 526
BstKTI GATC 4 cut(s) 178, 378, 515, 542
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 4 cut(s) 175, 375, 512, 539
BstNSI RCATGY 2 cut(s) 14, 144
BstSFI CTRYAG 4 cut(s) 164, 480, 597, 608
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 2 cut(s) 143, 527
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuI GTATCC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 350, 526
BveI ACCTGC 2 cut(s) 157, 405
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 1 cut(s) 341
CviAII CATG 4 cut(s) 11, 67, 141, 457
CviJI RGCY 8 cut(s) 202, 274, 295, 479, 499, 572, 586, 620
CviKI_1 RGCY 8 cut(s) 202, 274, 295, 479, 499, 572, 586, 620
DpnI GATC 4 cut(s) 177, 377, 514, 541
DpnII GATC 4 cut(s) 175, 375, 512, 539
Eam1104I CTCTTC 2 cut(s) 39, 366
EarI CTCTTC 2 cut(s) 39, 366
Eco130I CCWWGG 2 cut(s) 318, 581
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 677
EcoO109I RGGNCCY 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 318, 581
EcoT22I ATGCAT 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 318, 581
FaeI CATG 4 cut(s) 14, 70, 144, 460
FaiI YATR 9 cut(s) 12, 68, 142, 449, 458, 482, 617, 636, 662
FalI AAGNNNNNCTT 2 cut(s) 21, 53
FaqI GGGAC 1 cut(s) 562
FatI CATG 4 cut(s) 10, 66, 140, 456
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 2 cut(s) 357, 533
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 1 cut(s) 173
GluI GCNGC 1 cut(s) 296
HapII CCGG 1 cut(s) 653
HgaI GACGC 1 cut(s) 341
Hin1II CATG 4 cut(s) 14, 70, 144, 460
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 3 cut(s) 153, 431, 593
HpaII CCGG 1 cut(s) 653
HphI GGTGA 2 cut(s) 497, 517
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 5 cut(s) 59, 150, 194, 553, 653
HpyAV CCTTC 1 cut(s) 404
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 4 cut(s) 144, 166, 414, 599
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 4 cut(s) 14, 70, 144, 460
Kpn2I TCCGGA 1 cut(s) 652
Kzo9I GATC 4 cut(s) 175, 375, 512, 539
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 5 cut(s) 152, 400, 488, 501, 666
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 173
MaeII ACGT 1 cut(s) 532
MalI GATC 4 cut(s) 177, 377, 514, 541
MboI GATC 4 cut(s) 175, 375, 512, 539
MboII GAAGA 6 cut(s) 56, 119, 148, 383, 527, 602
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 8 cut(s) 18, 83, 99, 280, 301, 380, 401, 424
MnlI CCTC 6 cut(s) 40, 190, 201, 334, 562, 686
Mph1103I ATGCAT 1 cut(s) 146
MroI TCCGGA 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 1 cut(s) 357
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 1 cut(s) 653
NdeII GATC 4 cut(s) 175, 375, 512, 539
NlaIII CATG 4 cut(s) 14, 70, 144, 460
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 2 cut(s) 14, 144
PaeR7I CTCGAG 1 cut(s) 677
PaqCI CACCTGC 1 cut(s) 405
PciI ACATGT 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 3 cut(s) 153, 431, 593
PflFI GACNNNGTC 1 cut(s) 313
PkrI GCNGC 1 cut(s) 297
PpuMI RGGWCCY 1 cut(s) 169
PscI ACATGT 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 169
PspPI GGNCC 1 cut(s) 169
PspPPI RGGWCCY 1 cut(s) 169
PspXI VCTCGAGB 1 cut(s) 677
PstI CTGCAG 2 cut(s) 168, 601
PsuI RGATCY 1 cut(s) 175
PsyI GACNNNGTC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 4 cut(s) 175, 375, 512, 539
Sau96I GGNCC 1 cut(s) 169
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 4 cut(s) 164, 480, 597, 608
Sfr274I CTCGAG 1 cut(s) 677
SinI GGWCC 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 677
SmlI CTYRAG 6 cut(s) 71, 148, 194, 338, 356, 677
SmoI CTYRAG 6 cut(s) 71, 148, 194, 338, 356, 677
Sse9I AATT 8 cut(s) 18, 83, 99, 280, 301, 380, 401, 424
SspMI CTAG 1 cut(s) 173
StyI CCWWGG 2 cut(s) 318, 581
TaiI ACGT 1 cut(s) 535
TaqI TCGA 5 cut(s) 7, 378, 538, 552, 678
TasI AATT 8 cut(s) 18, 83, 99, 280, 301, 380, 401, 424
TfiI GAWTC 3 cut(s) 153, 431, 593
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 5 cut(s) 55, 134, 218, 464, 677
Tth111I GACNNNGTC 1 cut(s) 313
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 2 cut(s) 301, 380
XceI RCATGY 2 cut(s) 14, 144
XhoI CTCGAG 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.