Rroxscaffold_3G00274260

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000159
Physical Location & Seq
Forward (+)
65898765 .. 65901775
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274260.1

Sequence Viewer

Length: 2604 bp
ATGGGGGAGTTTGTCACTAGCATTGCACAGAACGCCGTGACTGGGCTAGCTTCTTTTACTTATAAAGAAGTTTGCTTGGCATGGGGTGTCACAGATGAGCTAGCCAAGCTCAAGGTGACCATCGCAGATGTCCAAAAAGTGGTCCTCGATGCAGAGGAAAAGCAGAGGAAGAATCCCCATCTGACCGAGTGGTTAGGCAAGCTCAAAGATGTATGCTACGACGTCGATGATGTGCTTGATGAATTTGCATTCAAAGAGCTACAAATGCAAGTGCTGAACAATCGCGGCAATATTGTAAAAGTACAGGTACGCACATTCTTTTCGCGTTGGAACCCACTTTTGTTTAACATCAAAATGGCTCATAGAATCAAAGAGATTAGAGAAACGTTAGATAGAGTGGCAGCTGAAAAAGCTTCGTTTCCTCTTGAGGAACGAGCAGAAGATTGGCAGGGGGTGCACGTAAAGAGGGAGACTCACTCTTTTGTGCAAGTCTATGATGTTATTGGGAGAGAGGATGATAAAAAACAGATTATCATTCATCTCTTGAACTTAAGATTGAAAGATCCTGGTGTTCGTGAGAACGTTTCAGTTATTTCCATTTTGGGTTTAGGAGGTATGGGAAAGACCACCCTCGCCAAGTTGGTGTATAATGATAAAAGGGTAGTAAAAAACTTTGAGTTGAGAATGTGGGTGTATGTCTCCGACCACTTTGATATTAAAAGATTGGTTCGAGAAATTATCACTTCTGCAACTAACCAAAACTGTGGGGTTGATGAAAGTTTAGAACAATTGCAAAAGAAGTTACAAAATGTTTTAACTGATAAACATTTTTTGTTAGTGTTAGATGATGTTTGGGACAAGAGTCAAATTGGAGTAACAATTTCAAAATGGCTTGATTTAAAAACTCTTTTGAATGTTGGAGCCAATGAGAGTAAAATCATTGTCACAACACGCAATGAGTCGGTTACTTCGATTATGGGATCTGTATACGTACATCAATTAAGAGGTCTTCGCCACGAAGATTGTATGACCTTGTTCAGTCAAAGGGCATTTGGCCGAAGGGAAGAAGAGGAACGCTATCCACAACTACTGGAAATCGGAGACGATATTGCAGAGAAGTGTGGAGGGGTTCCTCTAGCCATAACCACCTTAGGGAGCATGCTCTGTTTAAAAAGAGAACAGCATGCTTGGTCTGAAGTAAGAGATAGCGACATTTGGAAACTGGAACAGGAGGACGACCACATATTTCCTGCCCTCAAATTGAGCTATGATGCATTGCCGCCCAACTTGAAACCATGCTTTGCCTTTTGCTCACTCTTTCCCAAAGACTACATATTCCGAAGTTCGGAATTGGTCCCCCTCTGGATGGCACATGGCTTTCTTCAATCTTCAAAAGGAAGCCGAGAGCCTGAAGAGATGGGACTAGACTACATAAGGCAGATATGTTCTAGATCATTGTTTCAAATTCACGAAGATTCAATTCATTTCATAGCCTTTGAAATGCATGATCTAGTTCATGACTTGGCCATCTCGGCGGCACAAGTAGAGTGCTCATCTGAAAATTTTCGCCCTTCTACCTCCAACCCTCACAAAAAAGTTCGACATGTGTCAATCTCGCGGGATGACTTGTGCATGAAGGAAGTACGAGTTCCTGATTACTTGCTTCAGCTAAAGAAAGTGTGGACCATTCTATTTCCAAATCCAGGAGATACAGTCATCAACAACAAACCTTTGTTGAAAACATGCATCAAGAGATTCAAGTACCTGCGGGCGCTAGATTTGAGTTGTTGGAGTCTTGAGAAGTTACCAAGTTCAATTGGTAATTTGAGTCACTTGAGATATCTGGACTTGTCTAGGAATCCAAGCATGCAAAAGCTCCCGGATTCCATTTGCGAGTTGCAAAATTTGCAGACATTGCTACTTTCAGGCTGTGAGAATCTCAATGGCTTACCCAAAGACATAGGGAGCTTGATCAACCTTAGAAAGTTGGTGTTCACCACAAGTCGGAAGGTTTTGCCCGAAGAAATCAAAAGCCTCACTTCTCTTCGACATTTCGAGGTCCACAACTGCAGTAATCTTGAATCCTTAGGTGAAGTGGTACAATCTCTCACTAGCCTTCGAATTCTTGTTATTAGCAATTGTGGAACTCTAGAGTCACTGCCACAGTTGAGTACACCATTAAAGGCCTTGGTCATCTCTGATTGTGAGAAGCTCGACTTGATGAGTTTGGGAGAAGGCATTCGAAGTCTTCGATCATTTTCCATCTCCAAAATGTCAAATTTGGAGGCTTTGCCCCATTGGCTTCAAGACTCCAAACCCAGTCTACAGAGCATTCGGATCGAACATTGCGTGCATCTCAAAGCACTTCCAGAGTGGCTGCAAAGCTTGATGATGCTGCAACAACTGGTCATAGAAGGTTGTCCTGAATTGTTGGCTTTGCCGGAGGGGATGCATCATCTCGTTGCCCTGAAAGAATTGAAGATCAATGACTGTTCTAAATTGGTTGAAAGTTGCAGAAGGAAAGAAGATCAGGACCGGTCCAAGATCACTGGTGTTGCTAAGATTACAATTGATGGAGAGATCATTGCATCAAGTGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

867

Amino Acids

98.79

Weight (kDa)

6.39

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 93 7e-20 Rx N-terminal domain
NB-ARC PF00931 192 - 354 4e-35 NB-ARC domain
WHD_DRP PF23559 439 - 509 2.3e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 581 - 780 4.4e-26 Leucine-rich repeat region
LRR_8 PF13855 588 - 644 8.8e-08 Leucine rich repeat
LRR_RPS2 PF23247 674 - 807 4.6e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 1773
AccB7I CCANNNNNTGG 1 cut(s) 139
AccI GTMKAC 2 cut(s) 987, 2323
AccII CGCG 3 cut(s) 285, 325, 1618
AciI CCGC 5 cut(s) 285, 1280, 1535, 1618, 1768
AclI AACGTT 2 cut(s) 386, 582
AclWI GGATC 3 cut(s) 557, 988, 2345
AcoI YGGCCR 2 cut(s) 1054, 1524
AcsI RAATTY 6 cut(s) 242, 1464, 1561, 1903, 2121, 2278
AcuI CTGAAG 3 cut(s) 1215, 1431, 1649
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 7 cut(s) 303, 309, 993, 1644, 1763, 2100, 2173
AfiI CCNNNNNNNGG 7 cut(s) 42, 139, 1152, 1345, 1366, 1703, 2004
AflII CTTAAG 1 cut(s) 550
AflIII ACRYGT 1 cut(s) 1603
AgeI ACCGGT 1 cut(s) 2535
AjnI CCWGG 2 cut(s) 565, 1702
Alw21I GWGCWC 2 cut(s) 459, 1553
Alw26I GTCTC 3 cut(s) 464, 703, 1095
Alw44I GTGCAC 1 cut(s) 455
AlwI GGATC 3 cut(s) 557, 988, 2345
AoxI GGCC 3 cut(s) 1054, 1524, 2184
ApaLI GTGCAC 1 cut(s) 455
ApeKI GCWGC 3 cut(s) 401, 2377, 2395
ApoI RAATTY 6 cut(s) 242, 1464, 1561, 1903, 2121, 2278
AsiGI ACCGGT 1 cut(s) 2535
Asp700I GAANNNNTTC 2 cut(s) 1563, 2238
AspLEI GCGC 1 cut(s) 1774
AspS9I GGNCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
AsuC2I CCSGG 1 cut(s) 1880
AsuHPI GGTGA 3 cut(s) 127, 1987, 2102
AsuII TTCGAA 2 cut(s) 2119, 2242
AsuNHI GCTAGC 2 cut(s) 46, 100
AvaII GGWCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
AxyI CCTNAGG 2 cut(s) 1150, 2086
BaeGI GKGCMC 1 cut(s) 459
BalI TGGCCA 1 cut(s) 1526
BbsI GAAGAC 2 cut(s) 1001, 2240
Bbv12I GWGCWC 2 cut(s) 459, 1553
BbvI GCAGC 3 cut(s) 413, 2364, 2382
BccI CCATC 7 cut(s) 128, 186, 1360, 1411, 1535, 2270, 2567
BceAI ACGGC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 2320, 2354
BciT130I CCWGG 2 cut(s) 567, 1704
BclI TGATCA 1 cut(s) 1971
BcnI CCSGG 1 cut(s) 1880
BcoDI GTCTC 3 cut(s) 464, 703, 1095
BfmI CTRYAG 2 cut(s) 2068, 2324
BfoI RGCGCY 1 cut(s) 1775
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 1773
BglI GCCNNNNNGGC 2 cut(s) 1532, 2299
BisI GCNGC 6 cut(s) 286, 402, 1280, 1536, 2378, 2396
BlsI GCNGC 6 cut(s) 287, 403, 1281, 1537, 2379, 2397
Bme1390I CCNGG 3 cut(s) 567, 1704, 1880
Bme18I GGWCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
BmgT120I GGNCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
BmiI GGNNCC 4 cut(s) 332, 922, 1131, 1356
BmrFI CCNGG 3 cut(s) 567, 1704, 1880
BmrI ACTGGG 2 cut(s) 51, 2313
BmsI GCATC 8 cut(s) 139, 1261, 1755, 2362, 2382, 2439, 2461, 2597
BmtI GCTAGC 2 cut(s) 50, 104
BmuI ACTGGG 2 cut(s) 51, 2313
BoxI GACNNNNGTC 2 cut(s) 861, 1606
BpiI GAAGAC 2 cut(s) 1001, 2240
BplI GAGNNNNNCTC 4 cut(s) 457, 489, 461, 493
Bpu14I TTCGAA 2 cut(s) 2119, 2242
BpuEI CTTGAG 4 cut(s) 95, 446, 1817, 1855
BpuMI CCSGG 1 cut(s) 1880
BsaAI YACGTR 2 cut(s) 460, 991
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 2187
BsaWI WCCGGW 1 cut(s) 2535
BsaXI ACNNNNNCTCC 2 cut(s) 1698, 1728
Bsc4I CCNNNNNNNGG 7 cut(s) 42, 139, 1152, 1345, 1366, 1703, 2004
Bse118I RCCGGY 1 cut(s) 2535
Bse1I ACTGG 6 cut(s) 46, 1095, 1227, 2319, 2409, 2554
Bse21I CCTNAGG 2 cut(s) 1150, 2086
Bse3DI GCAATG 6 cut(s) 21, 961, 1274, 1913, 2344, 2583
BseBI CCWGG 2 cut(s) 567, 1704
BseDI CCNNGG 1 cut(s) 2187
BseGI GGATG 4 cut(s) 520, 1371, 1627, 2454
BseLI CCNNNNNNNGG 7 cut(s) 42, 139, 1152, 1345, 1366, 1703, 2004
BseMI GCAATG 6 cut(s) 21, 961, 1274, 1913, 2344, 2583
BseNI ACTGG 6 cut(s) 46, 1095, 1227, 2319, 2409, 2554
BseSI GKGCMC 1 cut(s) 459
BseXI GCAGC 3 cut(s) 413, 2364, 2382
Bsh1236I CGCG 3 cut(s) 285, 325, 1618
BshFI GGCC 3 cut(s) 1056, 1526, 2186
BshTI ACCGGT 1 cut(s) 2535
BsiHKAI GWGCWC 2 cut(s) 459, 1553
BsiSI CCGG 3 cut(s) 1880, 2441, 2536
BslFI GGGAC 3 cut(s) 869, 1340, 1434
BslI CCNNNNNNNGG 7 cut(s) 42, 139, 1152, 1345, 1366, 1703, 2004
BsmAI GTCTC 3 cut(s) 464, 703, 1095
BsmBI CGTCTC 1 cut(s) 1095
BsmFI GGGAC 3 cut(s) 869, 1340, 1434
BsmI GAATGC 3 cut(s) 248, 2238, 2331
BsnI GGCC 3 cut(s) 1056, 1526, 2186
Bsp119I TTCGAA 2 cut(s) 2119, 2242
Bsp1286I GDGCHC 2 cut(s) 459, 1553
BspACI CCGC 5 cut(s) 285, 1280, 1535, 1618, 1768
BspANI GGCC 3 cut(s) 1056, 1526, 2186
BspFNI CGCG 3 cut(s) 285, 325, 1618
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 4 cut(s) 332, 922, 1131, 1356
BspMAI CTGCAG 1 cut(s) 2072
BspMI ACCTGC 1 cut(s) 1773
BspOI GCTAGC 2 cut(s) 50, 104
BspPI GGATC 3 cut(s) 557, 988, 2345
BspT104I TTCGAA 2 cut(s) 2119, 2242
BspTI CTTAAG 1 cut(s) 550
BsrDI GCAATG 6 cut(s) 21, 961, 1274, 1913, 2344, 2583
BsrFI RCCGGY 1 cut(s) 2535
BsrI ACTGG 6 cut(s) 46, 1095, 1227, 2319, 2409, 2554
BssAI RCCGGY 1 cut(s) 2535
BssECI CCNNGG 1 cut(s) 2187
BssNAI GTATAC 1 cut(s) 988
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 2187
Bst1107I GTATAC 1 cut(s) 988
Bst2UI CCWGG 2 cut(s) 567, 1704
Bst4CI ACNGT 4 cut(s) 764, 1714, 2166, 2492
Bst6I CTCTTC 3 cut(s) 1062, 1407, 2049
BstACI GRCGYC 1 cut(s) 222
BstAFI CTTAAG 1 cut(s) 550
BstAPI GCANNNNNTGC 3 cut(s) 454, 1906, 1915
BstBAI YACGTR 2 cut(s) 460, 991
BstBI TTCGAA 2 cut(s) 2119, 2242
BstC8I GCNNGC 8 cut(s) 48, 102, 200, 1160, 1185, 1770, 1868, 2351
BstDEI CTNAG 4 cut(s) 1150, 1979, 2086, 2559
BstEII GGTNACC 1 cut(s) 115
BstF5I GGATG 4 cut(s) 520, 1371, 1627, 2454
BstFNI CGCG 3 cut(s) 285, 325, 1618
BstH2I RGCGCY 1 cut(s) 1775
BstHHI GCGC 1 cut(s) 1774
BstMAI GTCTC 3 cut(s) 464, 703, 1095
BstMWI GCNNNNNNNGC 9 cut(s) 32, 106, 265, 410, 454, 1532, 1906, 1915, 2299
BstNI CCWGG 2 cut(s) 567, 1704
BstNSI RCATGY 5 cut(s) 1162, 1187, 1607, 1746, 1870
BstPAI GACNNNNGTC 2 cut(s) 861, 1606
BstPI GGTNACC 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 565, 1702, 1878
BstSFI CTRYAG 2 cut(s) 2068, 2324
BstSLI GKGCMC 1 cut(s) 459
BstSNI TACGTA 1 cut(s) 991
BstUI CGCG 3 cut(s) 285, 325, 1618
BstV1I GCAGC 3 cut(s) 413, 2364, 2382
BstV2I GAAGAC 2 cut(s) 1001, 2240
BstX2I RGATCY 2 cut(s) 562, 980
BstXI CCANNNNNNTGG 1 cut(s) 764
BstYI RGATCY 2 cut(s) 562, 980
BstZ17I GTATAC 1 cut(s) 988
Bsu36I CCTNAGG 2 cut(s) 1150, 2086
BsuRI GGCC 3 cut(s) 1056, 1526, 2186
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 4 cut(s) 520, 1371, 1627, 2454
BtsI GCAGTG 1 cut(s) 2156
BtsIMutI CAGTG 2 cut(s) 2156, 2547
BveI ACCTGC 1 cut(s) 1773
Cac8I GCNNGC 8 cut(s) 48, 102, 200, 1160, 1185, 1770, 1868, 2351
CciI TCATGA 1 cut(s) 1516
CfoI GCGC 1 cut(s) 1774
Cfr10I RCCGGY 1 cut(s) 2535
Cfr13I GGNCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
Csp6I GTAC 7 cut(s) 302, 308, 992, 1643, 1762, 2099, 2172
CspAI ACCGGT 1 cut(s) 2535
CspCI CAANNNNNGTGG 2 cut(s) 1004, 1039
CviQI GTAC 7 cut(s) 302, 308, 992, 1643, 1762, 2099, 2172
DdeI CTNAG 4 cut(s) 1150, 1979, 2086, 2559
DraI TTTAAA 2 cut(s) 900, 1170
EaeI YGGCCR 2 cut(s) 1054, 1524
Eam1104I CTCTTC 3 cut(s) 1062, 1407, 2049
EarI CTCTTC 3 cut(s) 1062, 1407, 2049
Eco105I TACGTA 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 2187
Eco147I AGGCCT 1 cut(s) 2186
Eco32I GATATC 1 cut(s) 1841
Eco47I GGWCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
Eco57I CTGAAG 3 cut(s) 1215, 1431, 1649
Eco81I CCTNAGG 2 cut(s) 1150, 2086
Eco91I GGTNACC 1 cut(s) 115
EcoO65I GGTNACC 1 cut(s) 115
EcoRI GAATTC 1 cut(s) 2121
EcoRII CCWGG 2 cut(s) 565, 1702
EcoRV GATATC 1 cut(s) 1841
EcoT14I CCWWGG 1 cut(s) 2187
EcoT22I ATGCAT 4 cut(s) 1276, 1506, 1748, 2454
ErhI CCWWGG 1 cut(s) 2187
Esp3I CGTCTC 1 cut(s) 1095
FalI AAGNNNNNCTT 4 cut(s) 2023, 2055, 2201, 2233
FaqI GGGAC 3 cut(s) 869, 1340, 1434
FauI CCCGC 2 cut(s) 1611, 1761
FbaI TGATCA 1 cut(s) 1971
FblI GTMKAC 2 cut(s) 987, 2323
Fnu4HI GCNGC 6 cut(s) 286, 402, 1280, 1536, 2378, 2396
FokI GGATG 4 cut(s) 527, 1378, 1634, 2461
Fsp4HI GCNGC 6 cut(s) 286, 402, 1280, 1536, 2378, 2396
GlaI GCGC 1 cut(s) 1773
GluI GCNGC 6 cut(s) 286, 402, 1280, 1536, 2378, 2396
HaeII RGCGCY 1 cut(s) 1775
HaeIII GGCC 3 cut(s) 1056, 1526, 2186
HapII CCGG 3 cut(s) 1880, 2441, 2536
HhaI GCGC 1 cut(s) 1774
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 1 cut(s) 1772
HinP1I GCGC 1 cut(s) 1772
HindIII AAGCTT 2 cut(s) 411, 2383
HpaII CCGG 3 cut(s) 1880, 2441, 2536
HphI GGTGA 3 cut(s) 127, 1987, 2102
Hpy166II GTNNAC 7 cut(s) 457, 988, 1683, 1995, 2062, 2174, 2324
Hpy8I GTNNAC 7 cut(s) 457, 988, 1683, 1995, 2062, 2174, 2324
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 8 cut(s) 1053, 1581, 1630, 2002, 2126, 2228, 2408, 2511
HpyCH4III ACNGT 4 cut(s) 764, 1714, 2166, 2492
HpyCH4IV ACGT 5 cut(s) 222, 386, 459, 582, 990
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 106, 265, 410, 454, 1532, 1906, 1915, 2299
HpyF3I CTNAG 4 cut(s) 1150, 1979, 2086, 2559
HpySE526I ACGT 5 cut(s) 222, 386, 459, 582, 990
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 1 cut(s) 1772
Ksp22I TGATCA 1 cut(s) 1971
LmnI GCTCC 4 cut(s) 920, 1155, 1881, 1965
Lsp1109I GCAGC 3 cut(s) 413, 2364, 2382
LweI GCATC 8 cut(s) 139, 1261, 1755, 2362, 2382, 2439, 2461, 2597
MaeII ACGT 5 cut(s) 222, 386, 459, 582, 990
MfeI CAATTG 4 cut(s) 788, 1815, 2137, 2568
MflI RGATCY 2 cut(s) 562, 980
MhlI GDGCHC 2 cut(s) 459, 1553
MlsI TGGCCA 1 cut(s) 1526
MluNI TGGCCA 1 cut(s) 1526
MlyI GAGTC 7 cut(s) 466, 871, 968, 1801, 1837, 2162, 2303
MmeI TCCRAC 6 cut(s) 308, 726, 898, 1605, 1769, 1985
Mox20I TGGCCA 1 cut(s) 1526
Mph1103I ATGCAT 4 cut(s) 1276, 1506, 1748, 2454
MroXI GAANNNNTTC 2 cut(s) 1563, 2238
MscI TGGCCA 1 cut(s) 1526
MseI TTAA 8 cut(s) 345, 551, 717, 815, 899, 1001, 1169, 2180
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 1526
MspA1I CMGCKG 1 cut(s) 404
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 3 cut(s) 1880, 2441, 2536
MspR9I CCNGG 3 cut(s) 567, 1704, 1880
MunI CAATTG 4 cut(s) 788, 1815, 2137, 2568
Mva1269I GAATGC 3 cut(s) 248, 2238, 2331
MvaI CCWGG 2 cut(s) 567, 1704
MvnI CGCG 3 cut(s) 285, 325, 1618
MwoI GCNNNNNNNGC 9 cut(s) 32, 106, 265, 410, 454, 1532, 1906, 1915, 2299
NciI CCSGG 1 cut(s) 1880
NheI GCTAGC 2 cut(s) 46, 100
NlaIV GGNNCC 4 cut(s) 332, 922, 1131, 1356
NmeAIII GCCGAG 2 cut(s) 1427, 1511
NmuCI GTSAC 7 cut(s) 13, 37, 88, 115, 943, 1829, 2154
NsiI ATGCAT 4 cut(s) 1276, 1506, 1748, 2454
NspI RCATGY 5 cut(s) 1162, 1187, 1607, 1746, 1870
NspV TTCGAA 2 cut(s) 2119, 2242
PaeI GCATGC 3 cut(s) 1162, 1187, 1870
PagI TCATGA 1 cut(s) 1516
PceI AGGCCT 1 cut(s) 2186
PciI ACATGT 1 cut(s) 1603
PcsI WCGNNNNNNNCGW 3 cut(s) 968, 2248, 2346
PctI GAATGC 3 cut(s) 248, 2238, 2331
PdmI GAANNNNTTC 2 cut(s) 1563, 2238
PfeI GAWTC 8 cut(s) 172, 366, 1475, 1755, 1858, 1883, 1936, 2081
PflMI CCANNNNNTGG 1 cut(s) 139
PfoI TCCNGGA 2 cut(s) 1702, 1878
PinAI ACCGGT 1 cut(s) 2535
PkrI GCNGC 6 cut(s) 287, 403, 1281, 1537, 2379, 2397
PleI GAGTC 7 cut(s) 466, 870, 967, 1800, 1836, 2161, 2303
PpsI GAGTC 7 cut(s) 466, 870, 967, 1800, 1836, 2161, 2303
Ppu21I YACGTR 2 cut(s) 460, 991
PscI ACATGT 1 cut(s) 1603
PshAI GACNNNNGTC 2 cut(s) 861, 1606
PsiI TTATAA 1 cut(s) 63
Psp1406I AACGTT 2 cut(s) 386, 582
Psp6I CCWGG 2 cut(s) 565, 1702
PspEI GGTNACC 1 cut(s) 115
PspGI CCWGG 2 cut(s) 565, 1702
PspN4I GGNNCC 4 cut(s) 332, 922, 1131, 1356
PspPI GGNCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
PstI CTGCAG 1 cut(s) 2072
PsuI RGATCY 2 cut(s) 562, 980
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 7 cut(s) 303, 309, 993, 1644, 1763, 2100, 2173
RsaNI GTAC 7 cut(s) 302, 308, 992, 1643, 1762, 2099, 2172
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 8 cut(s) 345, 551, 717, 815, 899, 1001, 1169, 2180
SatI GCNGC 6 cut(s) 286, 402, 1280, 1536, 2378, 2396
Sau96I GGNCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
SchI GAGTC 7 cut(s) 466, 871, 968, 1801, 1837, 2162, 2303
ScrFI CCNGG 3 cut(s) 567, 1704, 1880
SduI GDGCHC 2 cut(s) 459, 1553
SfaNI GCATC 8 cut(s) 139, 1261, 1755, 2362, 2382, 2439, 2461, 2597
SfcI CTRYAG 2 cut(s) 2068, 2324
SfuI TTCGAA 2 cut(s) 2119, 2242
SinI GGWCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 5 cut(s) 110, 425, 550, 1796, 1834
SmoI CTYRAG 5 cut(s) 110, 425, 550, 1796, 1834
SnaBI TACGTA 1 cut(s) 991
SphI GCATGC 3 cut(s) 1162, 1187, 1870
SseBI AGGCCT 1 cut(s) 2186
SsiI CCGC 5 cut(s) 285, 1280, 1535, 1618, 1768
SspI AATATT 1 cut(s) 292
StuI AGGCCT 1 cut(s) 2186
StyD4I CCNGG 3 cut(s) 565, 1702, 1878
StyI CCWWGG 1 cut(s) 2187
TaaI ACNGT 4 cut(s) 764, 1714, 2166, 2492
TaiI ACGT 5 cut(s) 225, 389, 462, 585, 993
TaqII GACCGA 1 cut(s) 200
TatI WGTACW 2 cut(s) 301, 2171
TauI GCSGC 3 cut(s) 288, 1282, 1538
TfiI GAWTC 8 cut(s) 172, 366, 1475, 1755, 1858, 1883, 1936, 2081
Tru1I TTAA 8 cut(s) 345, 551, 717, 815, 899, 1001, 1169, 2180
Tru9I TTAA 8 cut(s) 345, 551, 717, 815, 899, 1001, 1169, 2180
TscAI CASTG 2 cut(s) 2163, 2554
TseFI GTSAC 7 cut(s) 13, 37, 88, 115, 943, 1829, 2154
TseI GCWGC 3 cut(s) 401, 2377, 2395
Tsp45I GTSAC 7 cut(s) 13, 37, 88, 115, 943, 1829, 2154
TspDTI ATGAA 7 cut(s) 255, 527, 789, 1472, 1477, 1505, 1649
TspRI CASTG 2 cut(s) 2163, 2554
Van91I CCANNNNNTGG 1 cut(s) 139
Vha464I CTTAAG 1 cut(s) 550
VneI GTGCAC 1 cut(s) 455
VpaK11BI GGWCC 6 cut(s) 142, 1354, 1683, 2059, 2533, 2538
XapI RAATTY 6 cut(s) 242, 1464, 1561, 1903, 2121, 2278
XbaI TCTAGA 2 cut(s) 1448, 2149
XceI RCATGY 5 cut(s) 1162, 1187, 1607, 1746, 1870
XmiI GTMKAC 2 cut(s) 987, 2323
XmnI GAANNNNTTC 2 cut(s) 1563, 2238
ZraI GACGTC 1 cut(s) 223
Zsp2I ATGCAT 4 cut(s) 1276, 1506, 1748, 2454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.