Rw2G021120

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000159
Physical Location & Seq
Forward (+)
29070573 .. 29072198
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021120.1

Sequence Viewer

Length: 1626 bp
ATGCATTGTTTAGAAGGTCTTCGCCATAAAGATTGCATGTCCCTGTTCATCCAAAGGGCATTTAAAAGAGGAGAGGAGCAGCGCTATCAACAATTGATAGAAATTGGAGAAGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCTATAGCTACCCTAGGGAGCATGCTCTATTTGAATACAGAGCGACACCATTGGTTGCGTGTCAGAGATGATGACATGTGGAGTATAGGGAATGACAATATTCTACCTGCACTCAAATTGAGCTATGATGCATTGCCACAACAGTTGAAACCGTGTTTTGTATTTTGTTCACTTTTCCCAAAGGATTATGAATTTGAGAGTCAAAATTTGGTTCCATTGTGGAGAGCACAAGGCTACCTCAAAACATCCAAGACAAATGAAGATTTTGAACAAATGGGTGTAGACTATATAATACAGTTTTGCTCTAGATCTTTGTTTCAAGTCGAGGCAGATCACAAAACCGTAATAAAGTTTAAAATACATGATCTTGTTCATGATTTAGCAATTTTGATGGCGCAAGTAGAGTACTCCACAACCAATTTTCGTCCCTCTAGTGCTTTTGAAATGGTTCGACATGTGTCAATATCCAAAACAGACTTGCTTGGAGAAGAGGCACAGGTTCTCGATTTCATGCTGAAGTCAAAGAAATTGCGAACTATTCTAGTTCCTGAAGATGGTCAGATGAGTCAACATTTTGTGAAGACATGCATCTCTAAATTCAAATATATGCGGGTTCTAGATCTCAGTGGTTCGCCTCTTGATGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATTTGAGATTTCTCAACTTGTCTTCTAATAGTAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGACCTTGTACCTTGCGGGTTGTCACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAACCTCCGAAGCTTGGTGATAACTACACAACAGATGTATTTGCCAAAAGGAATTAGACACCTCACCTTGCTTCAATTTCTATGTTTTGATGGATGTGTCAATCTTATATCTTTGGGCAAAGAGATCGAATTCCTCAACAACCTTCGCAATTTGTGGATTGGCAGGTGTAATAATTTGGAATCCTTGTCACCAAATATGAAACACATGACTACTTTAGATACTTTGGTTATCGATGATTGCGAGAAGCTTCAGGAGATGAGATCAGGAGAAGGTCCTCAAGGTCTTCGATCATTGGGTATCGGGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTTGAAGATTCTGCAGACACTCTACAGAGCACAGCACTTTCTGAATGTCATAGTCTCACAGTACTTCCAGAGTTGATAAACTTCGCATTCCTTGAGCAATTACACATTAAAGAATGCTCCAAATTGTCGGCTTTGCCACAAGGGTTGCATTCCCTTACTGAGTTGAGAGAACTGAATATTAAAGGGTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGGTGAAGATTACACTCGACTCTGATGTGGATGCTGATGATGAAGAAGGCGAAGATGAAGGTACACCAATGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

541

Amino Acids

61.51

Weight (kDa)

5.66

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 106 - 176 3.8e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 243 - 435 1.9e-27 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1113
Acc36I ACCTGC 2 cut(s) 259, 1113
AccI GTMKAC 1 cut(s) 426
AciI CCGC 2 cut(s) 754, 911
AcsI RAATTY 6 cut(s) 335, 349, 740, 889, 1088, 1264
AcuI CTGAAG 3 cut(s) 680, 714, 1193
AfaI GTAC 4 cut(s) 551, 905, 1356, 1609
AfeI AGCGCT 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 698, 973
AflIII ACRYGT 2 cut(s) 219, 598
AgsI TTSAA 9 cut(s) 178, 292, 413, 464, 587, 745, 1034, 1269, 1298
AjiI CACGTC 1 cut(s) 1544
AjuI GAANNNNNNNTTGG 2 cut(s) 1256, 1288
AluBI AGCT 8 cut(s) 146, 152, 267, 790, 883, 972, 1207, 1500
AluI AGCT 8 cut(s) 146, 152, 267, 790, 883, 972, 1207, 1500
Alw21I GWGCWC 2 cut(s) 373, 1325
Alw26I GTCTC 2 cut(s) 890, 1352
Aor51HI AGCGCT 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 79, 883, 1500
ApoI RAATTY 6 cut(s) 335, 349, 740, 889, 1088, 1264
Asp700I GAANNNNTTC 2 cut(s) 18, 591
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 2 cut(s) 84, 541
AspS9I GGNCC 1 cut(s) 1232
AsuHPI GGTGA 5 cut(s) 988, 1015, 1140, 1532, 1561
AvaII GGWCC 1 cut(s) 1232
AvrII CCTAGG 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BbsI GAAGAC 4 cut(s) 11, 731, 831, 1235
Bbv12I GWGCWC 2 cut(s) 373, 1325
BbvI GCAGC 3 cut(s) 91, 870, 1487
BccI CCATC 5 cut(s) 529, 692, 881, 1043, 1531
BcgI CGANNNNNNTGC 2 cut(s) 1066, 1100
BclI TGATCA 1 cut(s) 957
BcoDI GTCTC 2 cut(s) 890, 1352
BfaI CTAG 5 cut(s) 158, 450, 576, 686, 761
BfmI CTRYAG 3 cut(s) 147, 1305, 1316
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 2 cut(s) 259, 1113
BglI GCCNNNNNGGC 1 cut(s) 1291
BglII AGATCT 2 cut(s) 452, 763
BisI GCNGC 3 cut(s) 80, 884, 1501
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 3 cut(s) 81, 885, 1502
BmcAI AGTACT 2 cut(s) 551, 1356
Bme18I GGWCC 1 cut(s) 1232
BmgBI CACGTC 1 cut(s) 1544
BmgT120I GGNCC 1 cut(s) 1232
BmiI GGNNCC 2 cut(s) 357, 953
BmsI GCATC 3 cut(s) 262, 741, 1567
BoxI GACNNNNGTC 1 cut(s) 601
BpiI GAAGAC 4 cut(s) 11, 731, 831, 1235
BpuEI CTTGAG 3 cut(s) 947, 1221, 1406
Bsa29I ATCGAT 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 2 cut(s) 157, 1289
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 2 cut(s) 698, 973
Bse3DI GCAATG 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 1191
BseDI CCNNGG 2 cut(s) 157, 1289
BseGI GGATG 4 cut(s) 48, 389, 1058, 1582
BseLI CCNNNNNNNGG 2 cut(s) 698, 973
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 781, 1443
BseRI GAGGAG 5 cut(s) 84, 89, 147, 944, 1538
BseXI GCAGC 3 cut(s) 91, 870, 1487
BsgI GTGCAG 1 cut(s) 237
BshVI ATCGAT 1 cut(s) 1191
BsiHKAI GWGCWC 2 cut(s) 373, 1325
BslFI GGGAC 2 cut(s) 25, 555
BslI CCNNNNNNNGG 2 cut(s) 698, 973
BsmAI GTCTC 2 cut(s) 890, 1352
BsmFI GGGAC 2 cut(s) 25, 555
BsmI GAATGC 3 cut(s) 1379, 1412, 1441
Bso31I GGTCTC 1 cut(s) 890
Bsp1286I GDGCHC 2 cut(s) 373, 1325
Bsp143I GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
BspACI CCGC 2 cut(s) 754, 911
BspCNI CTCAG 2 cut(s) 780, 1444
BspDI ATCGAT 1 cut(s) 1191
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 2 cut(s) 357, 953
BspMAI CTGCAG 1 cut(s) 1309
BspMI ACCTGC 2 cut(s) 259, 1113
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 157, 1289
BssMI GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
BssT1I CCWWGG 2 cut(s) 157, 1289
Bst4CI ACNGT 5 cut(s) 288, 297, 441, 487, 1354
Bst6I CTCTTC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 1500
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 767, 1452
BstF5I GGATG 4 cut(s) 48, 389, 1058, 1582
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 2 cut(s) 84, 541
BstKTI GATC 9 cut(s) 455, 478, 511, 766, 960, 1086, 1223, 1250, 1620
BstMAI GTCTC 2 cut(s) 890, 1352
BstMBI GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
BstMWI GCNNNNNNNGC 2 cut(s) 1291, 1500
BstNSI RCATGY 5 cut(s) 40, 169, 223, 602, 732
BstPAI GACNNNNGTC 1 cut(s) 601
BstSFI CTRYAG 3 cut(s) 147, 1305, 1316
BstV1I GCAGC 3 cut(s) 91, 870, 1487
BstV2I GAAGAC 4 cut(s) 11, 731, 831, 1235
BstX2I RGATCY 2 cut(s) 452, 763
BstYI RGATCY 2 cut(s) 452, 763
Bsu15I ATCGAT 1 cut(s) 1191
BsuTUI ATCGAT 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 1544
BtsCI GGATG 4 cut(s) 48, 389, 1058, 1582
BtsIMutI CAGTG 1 cut(s) 775
BveI ACCTGC 2 cut(s) 259, 1113
Cac8I GCNNGC 1 cut(s) 167
CciI TCATGA 1 cut(s) 517
CfoI GCGC 2 cut(s) 84, 541
Cfr13I GGNCC 1 cut(s) 1232
ClaI ATCGAT 1 cut(s) 1191
Csp6I GTAC 4 cut(s) 550, 904, 1355, 1608
CspCI CAANNNNNGTGG 4 cut(s) 270, 305, 1419, 1454
CviAII CATG 9 cut(s) 37, 166, 220, 506, 518, 599, 655, 729, 1165
CviQI GTAC 4 cut(s) 550, 904, 1355, 1608
DdeI CTNAG 2 cut(s) 767, 1452
DpnI GATC 9 cut(s) 454, 477, 510, 765, 959, 1085, 1222, 1249, 1619
DpnII GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
DraI TTTAAA 2 cut(s) 64, 499
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 2 cut(s) 157, 1289
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 1 cut(s) 1232
Eco47III AGCGCT 1 cut(s) 83
Eco57I CTGAAG 3 cut(s) 680, 714, 1193
EcoO109I RGGNCCY 1 cut(s) 1232
EcoRI GAATTC 2 cut(s) 1088, 1264
EcoT14I CCWWGG 2 cut(s) 157, 1289
EcoT22I ATGCAT 3 cut(s) 6, 277, 734
ErhI CCWWGG 2 cut(s) 157, 1289
FaeI CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
FaqI GGGAC 2 cut(s) 25, 555
FatI CATG 9 cut(s) 36, 165, 219, 505, 517, 598, 654, 728, 1164
FauI CCCGC 2 cut(s) 747, 904
FbaI TGATCA 1 cut(s) 957
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 80, 884, 1501
FokI GGATG 4 cut(s) 35, 376, 1065, 1589
Fsp4HI GCNGC 3 cut(s) 80, 884, 1501
FspBI CTAG 5 cut(s) 158, 450, 576, 686, 761
GlaI GCGC 2 cut(s) 83, 540
GluI GCNGC 3 cut(s) 80, 884, 1501
HaeII RGCGCY 1 cut(s) 85
HhaI GCGC 2 cut(s) 84, 541
Hin1II CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
Hin6I GCGC 2 cut(s) 82, 539
HinP1I GCGC 2 cut(s) 82, 539
HincII GTYRAC 1 cut(s) 713
HindII GTYRAC 1 cut(s) 713
HindIII AAGCTT 2 cut(s) 970, 1205
HinfI GANTC 5 cut(s) 343, 709, 1139, 1301, 1565
HphI GGTGA 5 cut(s) 988, 1015, 1140, 1532, 1561
Hpy166II GTNNAC 4 cut(s) 314, 427, 713, 1610
Hpy188I TCNGA 5 cut(s) 209, 705, 968, 1336, 1570
Hpy8I GTNNAC 4 cut(s) 314, 427, 713, 1610
HpyAV CCTTC 5 cut(s) 8, 1112, 1223, 1586, 1598
HpyCH4III ACNGT 5 cut(s) 288, 297, 441, 487, 1354
HpyCH4IV ACGT 1 cut(s) 1543
HpyCH4V TGCA 8 cut(s) 4, 36, 254, 275, 732, 1307, 1441, 1503
HpyF10VI GCNNNNNNNGC 2 cut(s) 1291, 1500
HpyF3I CTNAG 2 cut(s) 767, 1452
HpySE526I ACGT 1 cut(s) 1543
Hsp92II CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
HspAI GCGC 2 cut(s) 82, 539
Ksp22I TGATCA 1 cut(s) 957
Kzo9I GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
LmnI GCTCC 3 cut(s) 76, 162, 1415
Lsp1109I GCAGC 3 cut(s) 91, 870, 1487
LweI GCATC 3 cut(s) 262, 741, 1567
MaeI CTAG 5 cut(s) 158, 450, 576, 686, 761
MaeII ACGT 1 cut(s) 1543
MaeIII GTNAC 2 cut(s) 917, 1146
MalI GATC 9 cut(s) 454, 477, 510, 765, 959, 1085, 1222, 1249, 1619
MboI GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
MfeI CAATTG 1 cut(s) 92
MflI RGATCY 2 cut(s) 452, 763
MhlI GDGCHC 2 cut(s) 373, 1325
MlyI GAGTC 3 cut(s) 352, 718, 1559
Mph1103I ATGCAT 3 cut(s) 6, 277, 734
MroXI GAANNNNTTC 2 cut(s) 18, 591
MseI TTAA 5 cut(s) 63, 498, 1401, 1473, 1514
MunI CAATTG 1 cut(s) 92
Mva1269I GAATGC 3 cut(s) 1379, 1412, 1441
MwoI GCNNNNNNNGC 2 cut(s) 1291, 1500
NdeII GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
NlaIII CATG 9 cut(s) 40, 169, 223, 509, 521, 602, 658, 732, 1168
NlaIV GGNNCC 2 cut(s) 357, 953
NmuCI GTSAC 2 cut(s) 917, 1146
NsiI ATGCAT 3 cut(s) 6, 277, 734
NspI RCATGY 5 cut(s) 40, 169, 223, 602, 732
PaeI GCATGC 1 cut(s) 169
PagI TCATGA 1 cut(s) 517
PaqCI CACCTGC 1 cut(s) 1113
PciI ACATGT 2 cut(s) 219, 598
PcsI WCGNNNNNNNCGW 1 cut(s) 1197
PctI GAATGC 3 cut(s) 1379, 1412, 1441
PdmI GAANNNNTTC 2 cut(s) 18, 591
PfeI GAWTC 2 cut(s) 1139, 1301
PkrI GCNGC 3 cut(s) 81, 885, 1502
PleI GAGTC 3 cut(s) 351, 717, 1559
PpsI GAGTC 3 cut(s) 351, 717, 1559
PpuMI RGGWCCY 1 cut(s) 1232
PscI ACATGT 2 cut(s) 219, 598
PshAI GACNNNNGTC 1 cut(s) 601
Psp5II RGGWCCY 1 cut(s) 1232
PspN4I GGNNCC 2 cut(s) 357, 953
PspPI GGNCC 1 cut(s) 1232
PspPPI RGGWCCY 1 cut(s) 1232
PstI CTGCAG 1 cut(s) 1309
PsuI RGATCY 2 cut(s) 452, 763
RsaI GTAC 4 cut(s) 551, 905, 1356, 1609
RsaNI GTAC 4 cut(s) 550, 904, 1355, 1608
SaqAI TTAA 5 cut(s) 63, 498, 1401, 1473, 1514
SatI GCNGC 3 cut(s) 80, 884, 1501
Sau3AI GATC 9 cut(s) 452, 475, 508, 763, 957, 1083, 1220, 1247, 1617
Sau96I GGNCC 1 cut(s) 1232
ScaI AGTACT 2 cut(s) 551, 1356
SchI GAGTC 3 cut(s) 352, 718, 1559
SduI GDGCHC 2 cut(s) 373, 1325
SfaNI GCATC 3 cut(s) 262, 741, 1567
SfcI CTRYAG 3 cut(s) 147, 1305, 1316
SinI GGWCC 1 cut(s) 1232
SmlI CTYRAG 3 cut(s) 926, 1236, 1385
SmoI CTYRAG 3 cut(s) 926, 1236, 1385
SphI GCATGC 1 cut(s) 169
SsiI CCGC 2 cut(s) 754, 911
SspI AATATT 2 cut(s) 244, 1471
SspMI CTAG 5 cut(s) 158, 450, 576, 686, 761
StyI CCWWGG 2 cut(s) 157, 1289
TaaI ACNGT 5 cut(s) 288, 297, 441, 487, 1354
TaiI ACGT 1 cut(s) 1546
TaqI TCGA 8 cut(s) 468, 595, 648, 1086, 1191, 1246, 1563, 1620
TatI WGTACW 2 cut(s) 549, 1354
TfiI GAWTC 2 cut(s) 1139, 1301
Tru1I TTAA 5 cut(s) 63, 498, 1401, 1473, 1514
Tru9I TTAA 5 cut(s) 63, 498, 1401, 1473, 1514
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 2 cut(s) 917, 1146
TseI GCWGC 3 cut(s) 79, 883, 1500
Tsp45I GTSAC 2 cut(s) 917, 1146
TspDTI ATGAA 9 cut(s) 37, 348, 417, 506, 643, 806, 1172, 1602, 1617
TspRI CASTG 1 cut(s) 775
VpaK11BI GGWCC 1 cut(s) 1232
XapI RAATTY 6 cut(s) 335, 349, 740, 889, 1088, 1264
XbaI TCTAGA 2 cut(s) 449, 760
XceI RCATGY 5 cut(s) 40, 169, 223, 602, 732
XmaJI CCTAGG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 18, 591
XspI CTAG 5 cut(s) 158, 450, 576, 686, 761
ZrmI AGTACT 2 cut(s) 551, 1356
Zsp2I ATGCAT 3 cut(s) 6, 277, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.