Rw7G002470

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000159
Physical Location & Seq
Forward (+)
2038357 .. 2040909
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002470.1

Sequence Viewer

Length: 2295 bp
ATGGGTCATAAAATCAAAGAGATTAGAGAAAGACTAGATGAGATTGATGCTGAAAAGACAAAGTTTTCTCTCATTCAAGTTGTTGAACTAGTTGATGATTGTGGTGAGCCCCAAAGGGCACATGTTAATAAAAGGGAGACAGACTCTTTACTGGAAGGTCATGTTGTTGGAAGAGATGGTGATAAGGAAAAAATTATCCTTCTCTTTGATGACACCCTTTCCTCCAGTGTCGATAATGTTTCAGTCATTTCCATCTTTGGGTTAGCAGGGTTGGGAAAAACCACACTCGCTAAATCAGTGTATAATGATACTGCGGTGGAGAAGAAATTTGATATTAGAATGTGGGTATGTGTCTCAGAAAACTTTGATATCCATACATTAGTTCGTGGCATTATTGCTACAACTAAACAAGAATGTGCTCGTGAAAGTTTAGATCTTATGAAAACGGGGTTGCAAGATATTTTGAGAGATAAAAAGTTTTTGTTAGTGTTGGACGATGTCTGGGATACAGAGTCGATAGGAGTTACAAATCAGAAATGGAGAGAGTTAAAAACTTTGTTAGATGTTGGAGACAGAGTAAGTAAAATCATCGTTACAACACGTAATGAATCAGTAGCTTCACTTGTGCACCCTATACATAAGCATAAGTTAGAAAGTCTTTCTCAGGAGGATTCCATGACTTTATTTAAAAACAATGCATTTAACGAAGGAGAGGAGTCACACTATCAACATTTGATAAAAATTGGAGAAGACATTGTGAAGAAGTGTGGAGGGGTTCCCTTATCACTAGCTACTCTTGGGAGTTTACTTCACTCAAAAAGGGAGCGACATCAGTGGTTACATGTAAGAGATAATGAAATATGGAGTGTGTCAGAAAACAACAACATTATGACTGCACTAAAATTGAGCTACAATGCACTGCCACCTCACTTGAAGCCTTGCTTTGCCTTTTGTTCACTTTACCCCAAGGATTATATATTTGGAAGTCAAGAAATAGTCCAATTATGGATGGCACAAGGCTTCCTCAAGTCCTCAAGAGAAAATGAAGATTTTGAAGAGATGGGCCTAGACTATATAAGGCAGTTTTGTTCCAAGTCTTTGTTTCAACTTGATATTGATATTGATTTGAAGACTACTGTGCAGTTCAAAATCCATGATTTAGTGCATGACTTGGCAATATCAGTGGCACGGGTAGATTGCTCCTCAATTAATTTCCGTCCCTCTAGTGCTTTTGAAAAAGTCCGACACGTGTCAATATCGAAAAAAGACTTGTCTGGGGATGTGGCAGGAGTCCCGGCATTCAATCTCCGGTCGGAGAAACTGCGGACCATTCTAAATGTAGATAGTGAAGCTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTGCGTGCGCTAGATGTGCGTGGTTCTACTCTTGAGGAGCTACCAAGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAACCTGGATTTTAATGAGAAGATAAAACAGCTTCCCAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTCAAGGTTGTGAGGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAATCTCAGATATTTGAATATAACTACACAGCAGATGTATTTTCCGAAAGGAGTATTTAGACGCCTCACCTTACTTCAATCCTTGCTCATTGGGAATTGTGCGAATCTGAAATCTCTGGGAGAAGAGATCCAATACCTCACTAACCTTCGTCAACTGTGGATCCGTTACTGTGGAAATCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCATTCCATACTTTGGCGATCGTTGAGTGTAAGAAGCTTGAGTTAATGAGGTCGGGAGAAGGTATCAATGGTCTCAGATCATTCGCTGTCGTCACATCAGATTTGGAGGCTTTGCCCAATTGGCTTCAAGAGTCTGCAGACACTTTACAGAGCATCTGGATCGGAGAGTGTGATAATCTCACGGCACTTCCAGAGTGGTTACAAAATCTCACCTTGCTGGAGCAACTGGCGATTGGGGAATGCCCCAAATTGTCTGCTTTGCCGCAAGGGATGCATCGCCTCACTGCCTTGAGAGTTTTGATGATTAGCGGGTGTCCTGAATTGAGAAAAAGATGCAAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAACATGTCCAAAGGATTGAACTCGATGGCGAGACGAGTTCTACTCTGACCAAAAGATTGGGAATCCAAATTTCAACTAATTACTCTCATCTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

764

Amino Acids

86.7

Weight (kDa)

6.43

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 73 - 236 1.1e-35 NB-ARC domain
WHD_DRP PF23559 320 - 392 1.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 463 - 663 5.1e-26 Leucine-rich repeat region
LRR_13 PF23286 477 - 624 9.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1840
AciI CCGC 4 cut(s) 314, 1324, 2090, 2136
AclWI GGATC 5 cut(s) 1364, 1735, 1768, 1781, 1994
AcsI RAATTY 3 cut(s) 326, 1543, 2265
AcvI CACGTG 1 cut(s) 1249
AcyI GRCGYC 1 cut(s) 1675
AfiI CCNNNNNNNGG 2 cut(s) 258, 1840
AflII CTTAAG 1 cut(s) 1555
AflIII ACRYGT 6 cut(s) 121, 599, 841, 1246, 1248, 2200
AhlI ACTAGT 1 cut(s) 88
AjnI CCWGG 1 cut(s) 1488
AluBI AGCT 8 cut(s) 617, 791, 909, 1352, 1444, 1516, 1537, 1864
AluI AGCT 8 cut(s) 617, 791, 909, 1352, 1444, 1516, 1537, 1864
Alw21I GWGCWC 2 cut(s) 421, 630
Alw26I GTCTC 5 cut(s) 131, 358, 564, 1904, 2222
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 5 cut(s) 1364, 1735, 1768, 1781, 1994
AoxI GGCC 1 cut(s) 1063
ApaLI GTGCAC 1 cut(s) 626
ApoI RAATTY 3 cut(s) 326, 1543, 2265
AseI ATTAAT 1 cut(s) 1209
AspLEI GCGC 1 cut(s) 1414
AspS9I GGNCC 3 cut(s) 1063, 1326, 2187
AsuC2I CCSGG 1 cut(s) 1295
AsuHPI GGTGA 4 cut(s) 116, 191, 1672, 2029
AvaII GGWCC 2 cut(s) 1326, 2187
BaeGI GKGCMC 2 cut(s) 121, 630
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BamHI GGATCC 1 cut(s) 1773
BanII GRGCYC 1 cut(s) 111
BauI CACGAG 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 1249
BbsI GAAGAC 2 cut(s) 756, 1136
Bbv12I GWGCWC 2 cut(s) 421, 630
BccI CCATC 5 cut(s) 170, 260, 1003, 1054, 2216
BceAI ACGGC 1 cut(s) 2025
BcgI CGANNNNNNTGC 2 cut(s) 1969, 2003
BciT130I CCWGG 1 cut(s) 1490
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 1 cut(s) 1611
BcnI CCSGG 1 cut(s) 1295
BcoDI GTCTC 5 cut(s) 131, 358, 564, 1904, 2222
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 6 cut(s) 35, 89, 788, 1067, 1224, 1415
BfmI CTRYAG 1 cut(s) 1962
BfrI CTTAAG 1 cut(s) 1555
BfuI GTATCC 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 1948
BglII AGATCT 1 cut(s) 433
BisI GCNGC 1 cut(s) 2090
BlsI GCNGC 1 cut(s) 2091
Bme1390I CCNGG 2 cut(s) 1295, 1490
Bme18I GGWCC 2 cut(s) 1326, 2187
BmgT120I GGNCC 3 cut(s) 1063, 1326, 2187
BmiI GGNNCC 3 cut(s) 777, 1607, 1775
BmrFI CCNGG 2 cut(s) 1295, 1490
BmrI ACTGGG 1 cut(s) 1515
BmsI GCATC 6 cut(s) 37, 1395, 1989, 2088, 2110, 2150
BmuI ACTGGG 1 cut(s) 1515
BoxI GACNNNNGTC 1 cut(s) 1249
BpiI GAAGAC 2 cut(s) 756, 1136
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 2224, 2256
BpmI CTGGAG 3 cut(s) 208, 1814, 2066
BpuEI CTTGAG 7 cut(s) 1010, 1018, 1411, 1457, 1601, 1886, 2137
BpuMI CCSGG 1 cut(s) 1295
BsaAI YACGTR 2 cut(s) 602, 1249
BsaHI GRCGYC 1 cut(s) 1675
BsaI GGTCTC 1 cut(s) 1904
BsaJI CCNNGG 1 cut(s) 966
BsaWI WCCGGW 1 cut(s) 1308
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 1840
Bse1I ACTGG 4 cut(s) 156, 225, 1521, 2058
BseBI CCWGG 1 cut(s) 1490
BseDI CCNNGG 1 cut(s) 966
BseGI GGATG 3 cut(s) 1014, 1285, 2103
BseLI CCNNNNNNNGG 2 cut(s) 258, 1840
BseMII CTCAG 4 cut(s) 369, 677, 1633, 1915
BseNI ACTGG 4 cut(s) 156, 225, 1521, 2058
BseRI GAGGAG 4 cut(s) 728, 1192, 1454, 1598
BseSI GKGCMC 2 cut(s) 121, 630
BseYI CCCAGC 1 cut(s) 1352
BsgI GTGCAG 2 cut(s) 879, 1160
Bsh1285I CGRYCG 2 cut(s) 1313, 1848
BshFI GGCC 1 cut(s) 1065
BsiEI CGRYCG 2 cut(s) 1313, 1848
BsiHKAI GWGCWC 2 cut(s) 421, 630
BsiSI CCGG 2 cut(s) 1295, 1309
BslFI GGGAC 2 cut(s) 1203, 1277
BslI CCNNNNNNNGG 2 cut(s) 258, 1840
BsmAI GTCTC 5 cut(s) 131, 358, 564, 1904, 2222
BsmBI CGTCTC 1 cut(s) 2222
BsmFI GGGAC 2 cut(s) 1203, 1277
BsmI GAATGC 3 cut(s) 1298, 1829, 2072
BsnI GGCC 1 cut(s) 1065
Bso31I GGTCTC 1 cut(s) 1904
Bsp1286I GDGCHC 4 cut(s) 111, 121, 421, 630
Bsp143I GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
BspACI CCGC 4 cut(s) 314, 1324, 2090, 2136
BspANI GGCC 1 cut(s) 1065
BspCNI CTCAG 4 cut(s) 368, 676, 1632, 1914
BspLI GGNNCC 3 cut(s) 777, 1607, 1775
BspMAI CTGCAG 1 cut(s) 1966
BspPI GGATC 5 cut(s) 1364, 1735, 1768, 1781, 1994
BspTI CTTAAG 1 cut(s) 1555
BspTNI GGTCTC 1 cut(s) 1904
BsrI ACTGG 4 cut(s) 156, 225, 1521, 2058
BssECI CCNNGG 1 cut(s) 966
BssMI GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
BssNI GRCGYC 1 cut(s) 1675
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 966
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 1490
Bst4CI ACNGT 3 cut(s) 1138, 1770, 1784
Bst6I CTCTTC 3 cut(s) 166, 1050, 1731
BstACI GRCGYC 1 cut(s) 1675
BstAFI CTTAAG 1 cut(s) 1555
BstAPI GCANNNNNTGC 1 cut(s) 2098
BstBAI YACGTR 2 cut(s) 602, 1249
BstC8I GCNNGC 2 cut(s) 1410, 1535
BstDEI CTNAG 4 cut(s) 355, 663, 1619, 1901
BstF5I GGATG 3 cut(s) 1014, 1285, 2103
BstHHI GCGC 1 cut(s) 1414
BstKTI GATC 9 cut(s) 436, 1359, 1614, 1743, 1776, 1848, 1907, 1989, 2196
BstMAI GTCTC 5 cut(s) 131, 358, 564, 1904, 2222
BstMBI GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
BstMCI CGRYCG 2 cut(s) 1313, 1848
BstMWI GCNNNNNNNGC 3 cut(s) 1420, 1948, 2098
BstNI CCWGG 1 cut(s) 1490
BstNSI RCATGY 4 cut(s) 125, 845, 1386, 2204
BstPAI GACNNNNGTC 1 cut(s) 1249
BstSCI CCNGG 2 cut(s) 1293, 1488
BstSFI CTRYAG 1 cut(s) 1962
BstSLI GKGCMC 2 cut(s) 121, 630
BstV2I GAAGAC 2 cut(s) 756, 1136
BstX2I RGATCY 4 cut(s) 433, 1356, 1740, 1773
BstXI CCANNNNNNTGG 1 cut(s) 2254
BstYI RGATCY 4 cut(s) 433, 1356, 1740, 1773
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 1 cut(s) 1065
BtgZI GCGATG 1 cut(s) 2087
BtsCI GGATG 3 cut(s) 1014, 1285, 2103
BtsI GCAGTG 2 cut(s) 917, 2109
BtsIMutI CAGTG 6 cut(s) 232, 303, 839, 917, 1188, 2109
Cac8I GCNNGC 2 cut(s) 1410, 1535
CfoI GCGC 1 cut(s) 1414
Cfr13I GGNCC 3 cut(s) 1063, 1326, 2187
CseI GACGC 1 cut(s) 1683
CspCI CAANNNNNGTGG 4 cut(s) 912, 947, 1164, 1199
CviAII CATG 9 cut(s) 122, 161, 676, 842, 1154, 1166, 1383, 1813, 2201
DdeI CTNAG 4 cut(s) 355, 663, 1619, 1901
DpnI GATC 9 cut(s) 435, 1358, 1613, 1742, 1775, 1847, 1906, 1988, 2195
DpnII GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
DraI TTTAAA 1 cut(s) 688
Eam1104I CTCTTC 3 cut(s) 166, 1050, 1731
EarI CTCTTC 3 cut(s) 166, 1050, 1731
Eco130I CCWWGG 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 1904
Eco32I GATATC 2 cut(s) 370, 1481
Eco47I GGWCC 2 cut(s) 1326, 2187
Eco72I CACGTG 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 1488
EcoRV GATATC 2 cut(s) 370, 1481
EcoT14I CCWWGG 1 cut(s) 966
EcoT22I ATGCAT 3 cut(s) 700, 1388, 2103
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 966
Esp3I CGTCTC 1 cut(s) 2222
FaeI CATG 9 cut(s) 125, 164, 679, 845, 1157, 1169, 1386, 1816, 2204
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 2 cut(s) 1203, 1277
FatI CATG 9 cut(s) 121, 160, 675, 841, 1153, 1165, 1382, 1812, 2200
FauI CCCGC 1 cut(s) 2129
FbaI TGATCA 1 cut(s) 1611
Fnu4HI GCNGC 1 cut(s) 2090
FokI GGATG 3 cut(s) 1021, 1292, 2110
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 2090
FspBI CTAG 6 cut(s) 35, 89, 788, 1067, 1224, 1415
GlaI GCGC 1 cut(s) 1413
GluI GCNGC 1 cut(s) 2090
GsaI CCCAGC 1 cut(s) 1356
GsuI CTGGAG 3 cut(s) 208, 1814, 2066
HaeIII GGCC 1 cut(s) 1065
HapII CCGG 2 cut(s) 1295, 1309
HgaI GACGC 1 cut(s) 1683
HhaI GCGC 1 cut(s) 1414
Hin1I GRCGYC 1 cut(s) 1675
Hin1II CATG 9 cut(s) 125, 164, 679, 845, 1157, 1169, 1386, 1816, 2204
Hin6I GCGC 1 cut(s) 1412
HinP1I GCGC 1 cut(s) 1412
HincII GTYRAC 1 cut(s) 1766
HindII GTYRAC 1 cut(s) 1766
HindIII AAGCTT 1 cut(s) 1862
HpaII CCGG 2 cut(s) 1295, 1309
HphI GGTGA 4 cut(s) 116, 191, 1672, 2029
Hpy166II GTNNAC 4 cut(s) 628, 806, 956, 1766
Hpy8I GTNNAC 4 cut(s) 628, 806, 956, 1766
HpyAV CCTTC 6 cut(s) 149, 209, 701, 1571, 1769, 1880
HpyCH4III ACNGT 3 cut(s) 1138, 1770, 1784
HpyCH4IV ACGT 2 cut(s) 601, 1248
HpyF10VI GCNNNNNNNGC 3 cut(s) 1420, 1948, 2098
HpyF3I CTNAG 4 cut(s) 355, 663, 1619, 1901
HpySE526I ACGT 2 cut(s) 601, 1248
Hsp92I GRCGYC 1 cut(s) 1675
Hsp92II CATG 9 cut(s) 125, 164, 679, 845, 1157, 1169, 1386, 1816, 2204
HspAI GCGC 1 cut(s) 1412
Ksp22I TGATCA 1 cut(s) 1611
Kzo9I GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
LmnI GCTCC 4 cut(s) 823, 1205, 1441, 2047
LweI GCATC 6 cut(s) 37, 1395, 1989, 2088, 2110, 2150
MaeI CTAG 6 cut(s) 35, 89, 788, 1067, 1224, 1415
MaeII ACGT 2 cut(s) 601, 1248
MaeIII GTNAC 9 cut(s) 523, 592, 717, 837, 1460, 1587, 1778, 1918, 2025
MalI GATC 9 cut(s) 435, 1358, 1613, 1742, 1775, 1847, 1906, 1988, 2195
MboI GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
MfeI CAATTG 1 cut(s) 1945
MflI RGATCY 4 cut(s) 433, 1356, 1740, 1773
MhlI GDGCHC 4 cut(s) 111, 121, 421, 630
MlyI GAGTC 6 cut(s) 137, 521, 725, 1299, 1805, 1967
MmeI TCCRAC 5 cut(s) 148, 471, 547, 1267, 1293
Mph1103I ATGCAT 3 cut(s) 700, 1388, 2103
MseI TTAA 9 cut(s) 126, 548, 687, 702, 1209, 1475, 1497, 1556, 1871
MspCI CTTAAG 1 cut(s) 1555
MspI CCGG 2 cut(s) 1295, 1309
MspR9I CCNGG 2 cut(s) 1295, 1490
MunI CAATTG 1 cut(s) 1945
Mva1269I GAATGC 3 cut(s) 1298, 1829, 2072
MvaI CCWGG 1 cut(s) 1490
MwoI GCNNNNNNNGC 3 cut(s) 1420, 1948, 2098
NciI CCSGG 1 cut(s) 1295
NdeII GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
NlaIII CATG 9 cut(s) 125, 164, 679, 845, 1157, 1169, 1386, 1816, 2204
NlaIV GGNNCC 3 cut(s) 777, 1607, 1775
NmuCI GTSAC 2 cut(s) 717, 1918
NsiI ATGCAT 3 cut(s) 700, 1388, 2103
NspI RCATGY 4 cut(s) 125, 845, 1386, 2204
PciI ACATGT 3 cut(s) 121, 841, 2200
PctI GAATGC 3 cut(s) 1298, 1829, 2072
PfeI GAWTC 6 cut(s) 608, 671, 1395, 1468, 1717, 2259
PflFI GACNNNGTC 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 1840
PkrI GCNGC 1 cut(s) 2091
Ple19I CGATCG 1 cut(s) 1848
PleI GAGTC 6 cut(s) 137, 520, 724, 1298, 1804, 1966
PmaCI CACGTG 1 cut(s) 1249
PmlI CACGTG 1 cut(s) 1249
PpsI GAGTC 6 cut(s) 137, 520, 724, 1298, 1804, 1966
Ppu21I YACGTR 2 cut(s) 602, 1249
PscI ACATGT 3 cut(s) 121, 841, 2200
PshAI GACNNNNGTC 1 cut(s) 1249
PshBI ATTAAT 1 cut(s) 1209
Psp6I CCWGG 1 cut(s) 1488
PspCI CACGTG 1 cut(s) 1249
PspFI CCCAGC 1 cut(s) 1352
PspGI CCWGG 1 cut(s) 1488
PspN4I GGNNCC 3 cut(s) 777, 1607, 1775
PspPI GGNCC 3 cut(s) 1063, 1326, 2187
PstI CTGCAG 1 cut(s) 1966
PsuI RGATCY 4 cut(s) 433, 1356, 1740, 1773
PsyI GACNNNGTC 1 cut(s) 497
PvuI CGATCG 1 cut(s) 1848
SaqAI TTAA 9 cut(s) 126, 548, 687, 702, 1209, 1475, 1497, 1556, 1871
SatI GCNGC 1 cut(s) 2090
Sau3AI GATC 9 cut(s) 433, 1356, 1611, 1740, 1773, 1845, 1904, 1986, 2193
Sau96I GGNCC 3 cut(s) 1063, 1326, 2187
SchI GAGTC 6 cut(s) 137, 521, 725, 1299, 1805, 1967
ScrFI CCNGG 2 cut(s) 1295, 1490
SduI GDGCHC 4 cut(s) 111, 121, 421, 630
SfaNI GCATC 6 cut(s) 37, 1395, 1989, 2088, 2110, 2150
SfcI CTRYAG 1 cut(s) 1962
SinI GGWCC 2 cut(s) 1326, 2187
SmlI CTYRAG 8 cut(s) 1025, 1033, 1390, 1436, 1555, 1580, 1865, 2116
SmoI CTYRAG 8 cut(s) 1025, 1033, 1390, 1436, 1555, 1580, 1865, 2116
SpeI ACTAGT 1 cut(s) 88
SsiI CCGC 4 cut(s) 314, 1324, 2090, 2136
SspI AATATT 1 cut(s) 1370
SspMI CTAG 6 cut(s) 35, 89, 788, 1067, 1224, 1415
StyD4I CCNGG 2 cut(s) 1293, 1488
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 3 cut(s) 1138, 1770, 1784
TaiI ACGT 2 cut(s) 604, 1251
TaqI TCGA 4 cut(s) 231, 515, 1259, 2220
TauI GCSGC 1 cut(s) 2092
TfiI GAWTC 6 cut(s) 608, 671, 1395, 1468, 1717, 2259
Tru1I TTAA 9 cut(s) 126, 548, 687, 702, 1209, 1475, 1497, 1556, 1871
Tru9I TTAA 9 cut(s) 126, 548, 687, 702, 1209, 1475, 1497, 1556, 1871
TscAI CASTG 6 cut(s) 232, 303, 839, 924, 1188, 2116
TseFI GTSAC 2 cut(s) 717, 1918
Tsp45I GTSAC 2 cut(s) 717, 1918
TspDTI ATGAA 6 cut(s) 455, 621, 870, 1059, 1460, 1829
TspGWI ACGGA 2 cut(s) 1205, 1766
TspRI CASTG 6 cut(s) 232, 303, 839, 924, 1188, 2116
Tth111I GACNNNGTC 1 cut(s) 497
Van91I CCANNNNNTGG 1 cut(s) 1840
Vha464I CTTAAG 1 cut(s) 1555
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 1326, 2187
VspI ATTAAT 1 cut(s) 1209
XapI RAATTY 3 cut(s) 326, 1543, 2265
XceI RCATGY 4 cut(s) 125, 845, 1386, 2204
XspI CTAG 6 cut(s) 35, 89, 788, 1067, 1224, 1415
Zsp2I ATGCAT 3 cut(s) 700, 1388, 2103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.