Rh1DG100600

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000159
Physical Location & Seq
Forward (+)
18842073 .. 18847957
5885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG100600.1

Sequence Viewer

Length: 693 bp
ATGGTTCGGCATGTGTCAATATCTAAAAAAGACTTGCTTGGAGAAGAGGCACAAGTTCCCGATTTCATACTCAAGTCAAAGAAATTGCGAACCATTCTAGTTCCTGAAGATGGCAAGATGAGTCAACGTTTTGTGAAGGCATGCATCTCGAGATTCAAATATGTGCGGGCTTTAGCTTTTGATGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGACGAATGGTGATTCGTAATTGTAATAATTTGGAATCCTTGCCACCAAATATGAAACACATGACTGCTTTAGATACTTTGGGTGTCGGTGATTGCGAGAAGCTTCAGGTGATGATGAGATCAGGGGAAGGTCCTCAACGTCTTCGATCATTGATTATCGAGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTCATTGAAAATTCTGCGGACACTCTACAAAGCATAGCACTTGATGGATGTCATAATCTTACGGCACTTCCGGAGTTGATAAACTTCACACTCCTCGAGCAACTACACATTGAAGAATGCTCCAAATTGTCGGCTTTGCCGCAAGGGTTGCATTGCCTTACTGAGTTGAGAGGATTGAAGATTGAAGGGTGTCATGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

230

Amino Acids

25.94

Weight (kDa)

8.36

Isoelectric Point (pI)

68.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 123 - 183 1.9e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 3 cut(s) 166, 446, 569
AclI AACGTT 1 cut(s) 127
AclWI GGATC 1 cut(s) 211
AcsI RAATTY 2 cut(s) 400, 439
AcuI CTGAAG 2 cut(s) 126, 326
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 6 cut(s) 157, 405, 437, 542, 607, 614
AjiI CACGTC 1 cut(s) 683
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 3 cut(s) 176, 340, 639
AluI AGCT 3 cut(s) 176, 340, 639
AlwI GGATC 1 cut(s) 211
Ama87I CYCGRG 2 cut(s) 148, 524
Aor13HI TCCGGA 1 cut(s) 499
ApeKI GCWGC 1 cut(s) 639
ApoI RAATTY 2 cut(s) 400, 439
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 4 cut(s) 259, 338, 358, 671
AvaI CYCGRG 2 cut(s) 148, 524
AvaII GGWCC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 626
BccI CCATC 4 cut(s) 104, 176, 467, 670
BceAI ACGGC 1 cut(s) 507
BcgI CGANNNNNNTGC 3 cut(s) 129, 163, 669
BfaI CTAG 1 cut(s) 98
BglI GCCNNNNNGGC 1 cut(s) 427
BisI GCNGC 2 cut(s) 569, 640
BlsI GCNGC 2 cut(s) 570, 641
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 2 cut(s) 148, 524
BmgBI CACGTC 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 368
BmsI GCATC 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 580
BseAI TCCGGA 1 cut(s) 499
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 191, 482
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 1 cut(s) 582
BseRI GAGGAG 2 cut(s) 512, 677
BseXI GCAGC 1 cut(s) 626
BsiHKCI CYCGRG 2 cut(s) 148, 524
BsiSI CCGG 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 110
BsmI GAATGC 1 cut(s) 551
BsoBI CYCGRG 2 cut(s) 148, 524
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 216, 356, 383
BspACI CCGC 3 cut(s) 166, 446, 569
BspCNI CTCAG 1 cut(s) 583
BspEI TCCGGA 1 cut(s) 499
BspHI TCATGA 1 cut(s) 622
BspPI GGATC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 3 cut(s) 216, 356, 383
BssT1I CCWWGG 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 39, 207
BstAPI GCANNNNNTGC 2 cut(s) 577, 639
BstC8I GCNNGC 2 cut(s) 142, 168
BstDEI CTNAG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 191, 482
BstKTI GATC 3 cut(s) 219, 359, 386
BstMBI GATC 3 cut(s) 216, 356, 383
BstMWI GCNNNNNNNGC 3 cut(s) 427, 577, 639
BstNSI RCATGY 2 cut(s) 14, 144
BstV1I GCAGC 1 cut(s) 626
BstV2I GAAGAC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BtrI CACGTC 1 cut(s) 683
BtsCI GGATG 2 cut(s) 191, 482
Cac8I GCNNGC 2 cut(s) 142, 168
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 368
CviAII CATG 4 cut(s) 11, 141, 298, 623
CviJI RGCY 7 cut(s) 170, 176, 340, 416, 430, 563, 639
CviKI_1 RGCY 7 cut(s) 170, 176, 340, 416, 430, 563, 639
DdeI CTNAG 1 cut(s) 591
DpnI GATC 3 cut(s) 218, 358, 385
DpnII GATC 3 cut(s) 216, 356, 383
Eam1104I CTCTTC 2 cut(s) 39, 207
EarI CTCTTC 2 cut(s) 39, 207
Eco130I CCWWGG 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 126, 326
Eco88I CYCGRG 2 cut(s) 148, 524
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 425
EcoT22I ATGCAT 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 4 cut(s) 14, 144, 301, 626
FaiI YATR 9 cut(s) 12, 68, 142, 162, 290, 299, 464, 483, 624
FalI AAGNNNNNCTT 2 cut(s) 21, 53
FatI CATG 4 cut(s) 10, 140, 297, 622
FauI CCCGC 1 cut(s) 159
Fnu4HI GCNGC 2 cut(s) 569, 640
FokI GGATG 2 cut(s) 198, 489
Fsp4HI GCNGC 2 cut(s) 569, 640
FspBI CTAG 1 cut(s) 98
GluI GCNGC 2 cut(s) 569, 640
HapII CCGG 1 cut(s) 500
Hin1II CATG 4 cut(s) 14, 144, 301, 626
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 121, 153, 250, 272
HpaII CCGG 1 cut(s) 500
HphI GGTGA 4 cut(s) 259, 338, 358, 671
Hpy166II GTNNAC 1 cut(s) 125
Hpy188III TCNNGA 7 cut(s) 59, 104, 148, 150, 397, 500, 623
Hpy8I GTNNAC 1 cut(s) 125
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 4 cut(s) 130, 245, 359, 608
HpyCH4IV ACGT 3 cut(s) 127, 376, 682
HpyCH4V TGCA 3 cut(s) 144, 580, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 427, 577, 639
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 3 cut(s) 127, 376, 682
Hsp92II CATG 4 cut(s) 14, 144, 301, 626
Kpn2I TCCGGA 1 cut(s) 499
Kzo9I GATC 3 cut(s) 216, 356, 383
LmnI GCTCC 1 cut(s) 554
LpnPI CCDG 4 cut(s) 117, 329, 345, 513
Lsp1109I GCAGC 1 cut(s) 626
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 98
MaeII ACGT 3 cut(s) 127, 376, 682
MalI GATC 3 cut(s) 218, 358, 385
MboI GATC 3 cut(s) 216, 356, 383
MboII GAAGA 6 cut(s) 56, 119, 224, 371, 554, 619
MflI RGATCY 1 cut(s) 216
MluCI AATT 9 cut(s) 83, 221, 256, 265, 400, 439, 554, 626, 650
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 7 cut(s) 40, 236, 381, 406, 533, 593, 655
Mph1103I ATGCAT 1 cut(s) 146
MroI TCCGGA 1 cut(s) 499
MseI TTAA 2 cut(s) 198, 653
MspI CCGG 1 cut(s) 500
Mva1269I GAATGC 1 cut(s) 551
MwoI GCNNNNNNNGC 3 cut(s) 427, 577, 639
NdeII GATC 3 cut(s) 216, 356, 383
NlaIII CATG 4 cut(s) 14, 144, 301, 626
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 2 cut(s) 14, 144
PaeI GCATGC 1 cut(s) 144
PaeR7I CTCGAG 2 cut(s) 148, 524
PagI TCATGA 1 cut(s) 622
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 1 cut(s) 551
PfeI GAWTC 3 cut(s) 153, 250, 272
PkrI GCNGC 2 cut(s) 570, 641
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
PpuMI RGGWCCY 1 cut(s) 368
Psp1406I AACGTT 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 368
PspPI GGNCC 1 cut(s) 368
PspPPI RGGWCCY 1 cut(s) 368
PspXI VCTCGAGB 1 cut(s) 524
PsuI RGATCY 1 cut(s) 216
SaqAI TTAA 2 cut(s) 198, 653
SatI GCNGC 2 cut(s) 569, 640
Sau3AI GATC 3 cut(s) 216, 356, 383
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 1 cut(s) 130
SfaNI GCATC 1 cut(s) 153
Sfr274I CTCGAG 2 cut(s) 148, 524
SinI GGWCC 1 cut(s) 368
SlaI CTCGAG 2 cut(s) 148, 524
SmlI CTYRAG 3 cut(s) 71, 148, 524
SmoI CTYRAG 3 cut(s) 71, 148, 524
SphI GCATGC 1 cut(s) 144
Sse9I AATT 9 cut(s) 83, 221, 256, 265, 400, 439, 554, 626, 650
SsiI CCGC 3 cut(s) 166, 446, 569
SspMI CTAG 1 cut(s) 98
StyI CCWWGG 1 cut(s) 425
TaiI ACGT 3 cut(s) 130, 379, 685
TaqI TCGA 5 cut(s) 149, 238, 382, 396, 525
TasI AATT 9 cut(s) 83, 221, 256, 265, 400, 439, 554, 626, 650
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 3 cut(s) 153, 250, 272
Tru1I TTAA 2 cut(s) 198, 653
Tru9I TTAA 2 cut(s) 198, 653
TseI GCWGC 1 cut(s) 639
TspDTI ATGAA 3 cut(s) 55, 305, 639
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 400, 439
XceI RCATGY 2 cut(s) 14, 144
XhoI CTCGAG 2 cut(s) 148, 524
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.