RchiOBHm_Chr5g0059261

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000159
Physical Location & Seq
Reverse (-)
64151367 .. 64153997
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33586

Sequence Viewer

Length: 2631 bp
ATGGAGTTTGTCTACGGCATAGCTGAGACAGTCTTGGGTAGGCTAGCTTCACATGCTTCCCAAGAAGTCTCCTTGGCATGGGGTGCCCAACTTGAGCTCACCAAGCTCAACAAGACCTTATCTGCCATCAAACTAGTGCTCGAGGATGCAGAGAAGAAGCAAGTGAGGAATCCCCCAATCACTCGTTGGTTAGGAAATCTCAAAGATGTTTGCCATGACGTTGATGATGTCTTGGACAAACTGGAGTTCCAAAAGTTGTGGTTGAAAGTGGAGGTCGATAACTGTGGGAAGATCAAAGGAAAGGTACGCCAATCTTTTTCCCGTTGGAATCCAGTAGTGTTTAACTTCAAAATGGGACATAAAGTGAAAGAGATTAGAGAACGACTGGTTGAAATTGATAATGAAAAGAGAGAGTTTGCTCTCCTTGAGGTTGCCAAGATAGTTGAAGATCATCATGCGCCCCAAGGGATGCACGATAATAAAAGAGAGACTGACTCTTTAGTGGAGGCCTCAGATGTTATTGGGAGAGATAATGATAAAAAACAAATTATCAGCCATCTCTTAAATAACACTGATATCTCTAGTGAGGAGAATATTTCTATTATTTCTATCCTTGGTTTAGGAGGGTTGGGAAAGACAACTCTTGCTAAATTAGTGTATAATGATAGCATGGTAGAGGAGAATTTTGAGATGAAAATGTGGATATGTGTCTCAGAAAACTTTGATATCCGAACATTAATTCGTGGCATTATCAATGCTGCGATTAAACAAAAATGTGAGGATGAAAGTTTGGACCTCATGAAAAAAAGATTGCAAGATACTTTGCGAGGTAGAAAGTTTTTGTTAGTGTTGGATGATGTTTGGGATACGGAGTTAATTGGAGTAACGATAGAAAAATGGATTGGTTTAAAAAATCTGTTAAATGTGGGAGCTAATGGAAGTAGAATCATCATAACAACACGAAATAGTTCAATTGCTTTACTTGTGAATCCTGCATACATGCACCCCTTAGAAGGTCTTTCCCACAAAGAATGCATGTCCTTGTTCATTAAAAGGGTATTTAAGAAAGGAGAGGAGCAACGCTATCAACATTTGATAGAAATTGGAGAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAGTAGCTACTGTAGGGAGCATGCTCTATTTGAAAACAGAGCAACACCATTGGTTGCGTGTTAGAGATGATGACATGTGGAGCATAAGGAATGTCAATATTCTACCTGCACTCAAATTGAGCTATGACGCATTGCCACAACACTTGAAACCGTGTTTTGCATTTTGTTCACTTTTTCCAAAGGATTATTTATTCCAGGGTCACCTTTTGGTTCCACTGTGGACAGCACAAGGCTACCTCAAGACATCTGAGAAAAATGAAGATTTTGAACATATGGGTATAGACTATATAAGGCAGTTTTGCTCTAGATCTTTGTTTCAAGTGGAGATGGATCTCAAAACAACCATAAGTTTTAAAATACATGATCTAGTTCATGATTTAGCAATTTCAGTGGCACAAGTAGAGTACTCCACAATCAATTTCCGTCCCTCTAGTGCTTTGGAAATGGTTCGACATGTGTCAATATCAGAAAAAGACTTACTTGGAGAAGAGGCACAAGTTCCCGATTTCATTCTCAAGTCAAAGAAATTACGAACCATTCTAATTTTTGAAGATGGCCAGATGAATCAACGTTTTGTGAAGACATGCATCTCAAGATTCAAATATATGCGGGTGCTAGATCTTAGTGGTTCGCCTCTTGAGGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATTTGAGATTTCTCAACTTGTCTTCTAATAGTAAGATAAAAAGGCTTCCCAATTCCATCAGTAAGCTGCTGAATTTGGAGACCTTGTCCTTTTGGGGTTGCGAAGCACTTGAGGAGATACCCAAAGACATAGGAAACCTGATCAACCTCCGAAGCTTGGTGATAACTACACAACAAATGTATTTGCCGAAAGGAATTAGACGCCTCACCTTACTTCAATTTTTATATTTTCATGGATGTGTCAATCTTAAATCTTTGGGAGAAGAGATCGAATTCCTCAACAAGCTTCGCAATTTGTGGATTAGCAGTTGTAATAATTTGGAATCCTTGCCACCAAATATGAAACGCATGACTACTTTAGATACTTTGACTGTCGATGATTGCGAGGAGCTTCAGTTGATGATGAGATCAGGGGAAGGTCCTCAAGGTCTTCGATCATTGATTATCATGAAATCAAGTTTGGAGGCTTTGCCCCCTTGGCTTGAAGATTCTAGAGACACTCTACAGAGCATAGCAGTTTCTGAATGTCATAATCTCACGGCACTTCCAGAGTTGATAAACTTCAAATTCCTCGAGCAACTACACATTGAAGAATGCTCCAAATTGTCTGCTTTTCCGCAAGGGTTGCAGTGCCTTACTGAGTTGAGAGAATTGAACATTAAAGGGTGTACTAAATTGAGCAAAAGCTGCAAGAGGCAATTAAAAGGTGAGAATTGGTTGTCGAAGATGGCAAGTCATCCAAAGATTACACTCGACTCTGATGTGGATACTGATGATGAAGAAGACGAAGATGAAGGTACACCCATGATGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

876

Amino Acids

100.12

Weight (kDa)

6.04

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 93 7.9e-19 Rx N-terminal domain
NB-ARC PF00931 178 - 356 9.1e-33 NB-ARC domain
WHD_DRP PF23559 439 - 509 7.5e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 559 - 769 6.4e-27 Leucine-rich repeat region
LRR_13 PF23286 593 - 740 2.6e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_R13L1-DRL21 PF25019 746 - 804 1.5e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1258
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 12
AciI CCGC 2 cut(s) 1753, 2432
AclI AACGTT 1 cut(s) 1714
AclWI GGATC 1 cut(s) 1482
AcoI YGGCCR 1 cut(s) 1699
AcsI RAATTY 4 cut(s) 682, 1888, 2087, 2381
AcuI CTGAAG 1 cut(s) 2192
AcyI GRCGYC 1 cut(s) 2017
AfaI GTAC 4 cut(s) 306, 1550, 2485, 2614
AfiI CCNNNNNNNGG 3 cut(s) 78, 1013, 1972
AflIII ACRYGT 2 cut(s) 1218, 1597
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 1 cut(s) 1338
AjuI GAANNNNNNNTTGG 4 cut(s) 885, 917, 2258, 2290
Alw21I GWGCWC 2 cut(s) 99, 141
Alw26I GTCTC 6 cut(s) 20, 73, 482, 715, 1889, 2304
AlwI GGATC 1 cut(s) 1482
AlwNI CAGNNNCTG 1 cut(s) 2336
Ama87I CYCGRG 2 cut(s) 140, 2387
AoxI GGCC 2 cut(s) 507, 1699
ApeKI GCWGC 3 cut(s) 758, 1882, 2502
ApoI RAATTY 4 cut(s) 682, 1888, 2087, 2381
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 967, 1590
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 793, 2234
AsuHPI GGTGA 5 cut(s) 91, 1337, 1987, 2014, 2534
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 2 cut(s) 140, 2387
AvaII GGWCC 2 cut(s) 793, 2234
BaeGI GKGCMC 1 cut(s) 88
BalI TGGCCA 1 cut(s) 1701
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 4 cut(s) 1730, 1830, 2237, 2604
Bbv12I GWGCWC 2 cut(s) 99, 141
BbvI GCAGC 3 cut(s) 745, 1869, 2489
BccI CCATC 7 cut(s) 134, 564, 1465, 1691, 1880, 2536, 2617
BceAI ACGGC 2 cut(s) 31, 2370
BciT130I CCWGG 1 cut(s) 1340
BciVI GTATCC 2 cut(s) 859, 2575
BclI TGATCA 1 cut(s) 1956
BcoDI GTCTC 6 cut(s) 20, 73, 482, 715, 1889, 2304
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 8 cut(s) 44, 134, 582, 1449, 1511, 1575, 1760, 2307
BfmI CTRYAG 2 cut(s) 1155, 2318
BfuAI ACCTGC 1 cut(s) 1258
BfuI GTATCC 2 cut(s) 859, 2575
BglI GCCNNNNNGGC 1 cut(s) 2293
BglII AGATCT 2 cut(s) 1451, 1762
BisI GCNGC 3 cut(s) 759, 1883, 2503
BlsI GCNGC 3 cut(s) 760, 1884, 2504
BmcAI AGTACT 1 cut(s) 1550
Bme1390I CCNGG 1 cut(s) 1340
Bme18I GGWCC 2 cut(s) 793, 2234
BmeT110I CYCGRG 2 cut(s) 140, 2387
BmgT120I GGNCC 2 cut(s) 793, 2234
BmiI GGNNCC 2 cut(s) 85, 1356
BmrFI CCNGG 1 cut(s) 1340
BmsI GCATC 3 cut(s) 136, 459, 1740
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1600
BpiI GAAGAC 4 cut(s) 1730, 1830, 2237, 2604
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 263
BpuEI CTTGAG 8 cut(s) 113, 446, 1367, 1643, 1720, 1802, 1946, 2223
BsaHI GRCGYC 1 cut(s) 2017
BsaI GGTCTC 1 cut(s) 1889
BsaJI CCNNGG 5 cut(s) 72, 463, 613, 1339, 2291
BsaXI ACNNNNNCTCC 4 cut(s) 1886, 1916, 2195, 2225
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1013, 1972
Bse1I ACTGG 3 cut(s) 246, 332, 390
Bse3DI GCAATG 1 cut(s) 1274
BseBI CCWGG 1 cut(s) 1340
BseDI CCNNGG 5 cut(s) 72, 463, 613, 1339, 2291
BseGI GGATG 6 cut(s) 151, 474, 787, 859, 2057, 2551
BseLI CCNNNNNNNGG 3 cut(s) 78, 1013, 1972
BseMI GCAATG 1 cut(s) 1274
BseMII CTCAG 5 cut(s) 15, 525, 726, 1383, 2445
BseNI ACTGG 3 cut(s) 246, 332, 390
BseRI GAGGAG 7 cut(s) 602, 692, 1088, 1146, 1799, 1943, 2216
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 3 cut(s) 745, 1869, 2489
BsgI GTGCAG 1 cut(s) 1236
BshFI GGCC 2 cut(s) 509, 1701
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 2 cut(s) 99, 141
BsiHKCI CYCGRG 2 cut(s) 140, 2387
BslFI GGGAC 2 cut(s) 369, 1554
BslI CCNNNNNNNGG 3 cut(s) 78, 1013, 1972
BsmAI GTCTC 6 cut(s) 20, 73, 482, 715, 1889, 2304
BsmFI GGGAC 2 cut(s) 369, 1554
BsmI GAATGC 2 cut(s) 1037, 2414
BsnI GGCC 2 cut(s) 509, 1701
Bso31I GGTCTC 1 cut(s) 1889
BsoBI CYCGRG 2 cut(s) 140, 2387
Bsp1286I GDGCHC 3 cut(s) 88, 99, 141
BspACI CCGC 2 cut(s) 1753, 2432
BspANI GGCC 2 cut(s) 509, 1701
BspCNI CTCAG 5 cut(s) 16, 524, 725, 1384, 2446
BspHI TCATGA 3 cut(s) 798, 1516, 2262
BspLI GGNNCC 2 cut(s) 85, 1356
BspMI ACCTGC 1 cut(s) 1258
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 1482
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 1889
BsrDI GCAATG 1 cut(s) 1274
BsrI ACTGG 3 cut(s) 246, 332, 390
BssECI CCNNGG 5 cut(s) 72, 463, 613, 1339, 2291
BssNI GRCGYC 1 cut(s) 2017
BssT1I CCWWGG 4 cut(s) 72, 463, 613, 2291
Bst2UI CCWGG 1 cut(s) 1340
Bst4CI ACNGT 6 cut(s) 31, 284, 1156, 1296, 1362, 2188
Bst6I CTCTTC 2 cut(s) 1626, 2073
BstACI GRCGYC 1 cut(s) 2017
BstAPI GCANNNNNTGC 3 cut(s) 83, 2440, 2502
BstC8I GCNNGC 2 cut(s) 45, 1166
BstDEI CTNAG 7 cut(s) 24, 511, 712, 1009, 1392, 1766, 2454
BstEII GGTNACC 1 cut(s) 1343
BstF5I GGATG 6 cut(s) 151, 474, 787, 859, 2057, 2551
BstHHI GCGC 1 cut(s) 460
BstMAI GTCTC 6 cut(s) 20, 73, 482, 715, 1889, 2304
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2440, 2502
BstNI CCWGG 1 cut(s) 1340
BstNSI RCATGY 7 cut(s) 56, 1003, 1039, 1168, 1222, 1601, 1731
BstPAI GACNNNNGTC 1 cut(s) 1600
BstPI GGTNACC 1 cut(s) 1343
BstSCI CCNGG 1 cut(s) 1338
BstSFI CTRYAG 2 cut(s) 1155, 2318
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 3 cut(s) 745, 1869, 2489
BstV2I GAAGAC 4 cut(s) 1730, 1830, 2237, 2604
BstX2I RGATCY 3 cut(s) 1451, 1474, 1762
BstYI RGATCY 3 cut(s) 1451, 1474, 1762
BsuI GTATCC 2 cut(s) 859, 2575
BsuRI GGCC 2 cut(s) 509, 1701
BtsCI GGATG 6 cut(s) 151, 474, 787, 859, 2057, 2551
BtsI GCAGTG 1 cut(s) 2450
BtsIMutI CAGTG 4 cut(s) 570, 1358, 1539, 2450
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 2 cut(s) 45, 1166
CaiI CAGNNNCTG 1 cut(s) 2336
CciI TCATGA 3 cut(s) 798, 1516, 2262
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 793, 2234
CseI GACGC 2 cut(s) 1280, 2025
Csp6I GTAC 4 cut(s) 305, 1549, 2484, 2613
CspCI CAANNNNNGTGG 6 cut(s) 239, 274, 1269, 1304, 1515, 1550
CviQI GTAC 4 cut(s) 305, 1549, 2484, 2613
DdeI CTNAG 7 cut(s) 24, 511, 712, 1009, 1392, 1766, 2454
DraI TTTAAA 2 cut(s) 909, 1498
EaeI YGGCCR 1 cut(s) 1699
Eam1104I CTCTTC 2 cut(s) 1626, 2073
EarI CTCTTC 2 cut(s) 1626, 2073
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 4 cut(s) 72, 463, 613, 2291
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 1889
Eco32I GATATC 2 cut(s) 577, 727
Eco47I GGWCC 2 cut(s) 793, 2234
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 2192
Eco88I CYCGRG 2 cut(s) 140, 2387
Eco91I GGTNACC 1 cut(s) 1343
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2234
EcoO65I GGTNACC 1 cut(s) 1343
EcoRI GAATTC 1 cut(s) 2087
EcoRII CCWGG 1 cut(s) 1338
EcoRV GATATC 2 cut(s) 577, 727
EcoT14I CCWWGG 4 cut(s) 72, 463, 613, 2291
EcoT22I ATGCAT 2 cut(s) 1037, 1733
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 4 cut(s) 72, 463, 613, 2291
FalI AAGNNNNNCTT 2 cut(s) 1608, 1640
FaqI GGGAC 2 cut(s) 369, 1554
FauI CCCGC 1 cut(s) 1746
FauNDI CATATG 1 cut(s) 1416
FbaI TGATCA 1 cut(s) 1956
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 759, 1883, 2503
FokI GGATG 6 cut(s) 158, 481, 794, 866, 2064, 2538
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 759, 1883, 2503
FspBI CTAG 8 cut(s) 44, 134, 582, 1449, 1511, 1575, 1760, 2307
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 759, 1883, 2503
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 2 cut(s) 509, 1701
HgaI GACGC 2 cut(s) 1280, 2025
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 1 cut(s) 2017
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 2 cut(s) 1969, 2099
HphI GGTGA 5 cut(s) 91, 1337, 1987, 2014, 2534
Hpy166II GTNNAC 5 cut(s) 13, 1313, 1365, 2484, 2615
Hpy188I TCNGA 8 cut(s) 514, 715, 731, 1393, 1612, 1967, 2338, 2575
Hpy8I GTNNAC 5 cut(s) 13, 1313, 1365, 2484, 2615
HpyAV CCTTC 3 cut(s) 1007, 2225, 2603
HpyCH4III ACNGT 6 cut(s) 31, 284, 1156, 1296, 1362, 2188
HpyCH4IV ACGT 2 cut(s) 219, 1714
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2440, 2502
HpyF3I CTNAG 7 cut(s) 24, 511, 712, 1009, 1392, 1766, 2454
HpySE526I ACGT 2 cut(s) 219, 1714
Hsp92I GRCGYC 1 cut(s) 2017
HspAI GCGC 1 cut(s) 458
Ksp22I TGATCA 1 cut(s) 1956
LmnI GCTCC 7 cut(s) 929, 1075, 1161, 1224, 1786, 2203, 2417
Lsp1109I GCAGC 3 cut(s) 745, 1869, 2489
LweI GCATC 3 cut(s) 136, 459, 1740
MaeI CTAG 8 cut(s) 44, 134, 582, 1449, 1511, 1575, 1760, 2307
MaeII ACGT 2 cut(s) 219, 1714
MaeIII GTNAC 2 cut(s) 883, 1343
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 3 cut(s) 1451, 1474, 1762
MhlI GDGCHC 3 cut(s) 88, 99, 141
MlsI TGGCCA 1 cut(s) 1701
MluNI TGGCCA 1 cut(s) 1701
MlyI GAGTC 2 cut(s) 488, 2564
MmeI TCCRAC 2 cut(s) 305, 831
Mox20I TGGCCA 1 cut(s) 1701
Mph1103I ATGCAT 2 cut(s) 1037, 1733
MroXI GAANNNNTTC 2 cut(s) 967, 1590
MscI TGGCCA 1 cut(s) 1701
MslI CAYNNNNRTG 1 cut(s) 2052
Msp20I TGGCCA 1 cut(s) 1701
MspR9I CCNGG 1 cut(s) 1340
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 2 cut(s) 1037, 2414
MvaI CCWGG 1 cut(s) 1340
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2440, 2502
NdeI CATATG 1 cut(s) 1416
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 2 cut(s) 85, 1356
NmuCI GTSAC 1 cut(s) 1343
NsiI ATGCAT 2 cut(s) 1037, 1733
NspI RCATGY 7 cut(s) 56, 1003, 1039, 1168, 1222, 1601, 1731
PaeI GCATGC 1 cut(s) 1168
PaeR7I CTCGAG 2 cut(s) 140, 2387
PagI TCATGA 3 cut(s) 798, 1516, 2262
PceI AGGCCT 1 cut(s) 509
PciI ACATGT 2 cut(s) 1218, 1597
PcsI WCGNNNNNNNCGW 1 cut(s) 2196
PctI GAATGC 2 cut(s) 1037, 2414
PdmI GAANNNNTTC 2 cut(s) 967, 1590
PfeI GAWTC 8 cut(s) 169, 328, 945, 988, 1708, 1740, 2138, 2303
PflFI GACNNNGTC 1 cut(s) 1900
PkrI GCNGC 3 cut(s) 760, 1884, 2504
PleI GAGTC 2 cut(s) 488, 2564
PpsI GAGTC 2 cut(s) 488, 2564
PpuMI RGGWCCY 1 cut(s) 2234
PscI ACATGT 2 cut(s) 1218, 1597
PshAI GACNNNNGTC 1 cut(s) 1600
PshBI ATTAAT 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 1714
Psp5II RGGWCCY 1 cut(s) 2234
Psp6I CCWGG 1 cut(s) 1338
PspEI GGTNACC 1 cut(s) 1343
PspGI CCWGG 1 cut(s) 1338
PspN4I GGNNCC 2 cut(s) 85, 1356
PspPI GGNCC 2 cut(s) 793, 2234
PspPPI RGGWCCY 1 cut(s) 2234
PspXI VCTCGAGB 2 cut(s) 140, 2387
PstNI CAGNNNCTG 1 cut(s) 2336
PsuI RGATCY 3 cut(s) 1451, 1474, 1762
PsyI GACNNNGTC 1 cut(s) 1900
RsaI GTAC 4 cut(s) 306, 1550, 2485, 2614
RsaNI GTAC 4 cut(s) 305, 1549, 2484, 2613
RseI CAYNNNNRTG 1 cut(s) 2052
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 3 cut(s) 759, 1883, 2503
Sau96I GGNCC 2 cut(s) 793, 2234
ScaI AGTACT 1 cut(s) 1550
SchI GAGTC 2 cut(s) 488, 2564
ScrFI CCNGG 1 cut(s) 1340
SduI GDGCHC 3 cut(s) 88, 99, 141
SfaNI GCATC 3 cut(s) 136, 459, 1740
SfcI CTRYAG 2 cut(s) 1155, 2318
Sfr274I CTCGAG 2 cut(s) 140, 2387
SinI GGWCC 2 cut(s) 793, 2234
SlaI CTCGAG 2 cut(s) 140, 2387
SmiMI CAYNNNNRTG 1 cut(s) 2052
SpeI ACTAGT 1 cut(s) 133
SphI GCATGC 1 cut(s) 1168
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 2 cut(s) 1753, 2432
SspI AATATT 2 cut(s) 595, 1243
SspMI CTAG 8 cut(s) 44, 134, 582, 1449, 1511, 1575, 1760, 2307
SstI GAGCTC 1 cut(s) 99
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 1338
StyI CCWWGG 4 cut(s) 72, 463, 613, 2291
TaaI ACNGT 6 cut(s) 31, 284, 1156, 1296, 1362, 2188
TaiI ACGT 2 cut(s) 222, 1717
TaqI TCGA 9 cut(s) 141, 276, 1594, 2085, 2190, 2248, 2388, 2537, 2568
TatI WGTACW 2 cut(s) 1548, 2483
TfiI GAWTC 8 cut(s) 169, 328, 945, 988, 1708, 1740, 2138, 2303
TscAI CASTG 4 cut(s) 577, 1365, 1539, 2450
TseFI GTSAC 1 cut(s) 1343
TseI GCWGC 3 cut(s) 758, 1882, 2502
Tsp45I GTSAC 1 cut(s) 1343
TspGWI ACGGA 2 cut(s) 884, 1556
TspRI CASTG 4 cut(s) 577, 1365, 1539, 2450
Tth111I GACNNNGTC 1 cut(s) 1900
VpaK11BI GGWCC 2 cut(s) 793, 2234
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 4 cut(s) 682, 1888, 2087, 2381
XbaI TCTAGA 2 cut(s) 1448, 2306
XceI RCATGY 7 cut(s) 56, 1003, 1039, 1168, 1222, 1601, 1731
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XhoI CTCGAG 2 cut(s) 140, 2387
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 2 cut(s) 967, 1590
XspI CTAG 8 cut(s) 44, 134, 582, 1449, 1511, 1575, 1760, 2307
ZrmI AGTACT 1 cut(s) 1550
Zsp2I ATGCAT 2 cut(s) 1037, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.