RchiOBHm_Chr2g0116981

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000159
Physical Location & Seq
Reverse (-)
29306605 .. 29312890
6286 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48999

Sequence Viewer

Length: 2649 bp
ATGGAGTTTGCCAGCAGCATTGCAGAGAACGTCTTGGATAGGCTAGCTTCACATGCTTCCCAAGAGATCTCCTTGGCATGGGGTGCTCAACTTGAGCTCACCAAGCTCGACAACACCTTATCTACCATCAAACTAGTGCTCGAGGATGCAGAGAAGAAGCAAGTGAGAAATCCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGATGTTTGCCATGACGTTGATGATGTCTTGGACAAACTAGACTTCCAAAAGTTGCGGTTGAAAGTGGAGGTCGACAACTGTGGGAAGATCAAAGGAAAGGTACGCCAATTTTTTTCCCGTTGGAATCCAGTACTGTTTAACTTCAGAATGGGACATAAAGTGAAAGAGATTAGAGAAAGACTGGTTGAAATTGATAATGAAAAGAGAGAGTTTGGTCTAATTGAGGTTGATAAGATAGTTGAAGATCATCATGCGCCCCAAGGGATGCACGATAATGAAAGAGAGACTGACTCTTTACTGGAGGCTACAGATGTTATTGGGAGAGATAATGATAAAAAACAGATTATTAGCCATCTCTTAAATAACAGTGATATCTCTAGTGAGGAGAATGTTTATGTTATTTCCATCCTTGGGTTAGGGGGGTTGGGAAAGACAACTCTTTCTAAATTAGTGTATAATGATAGGATGGTAGAGGAGAATTTTCAAATGAAAATGTGGGTATGTGTTTCAGAAAACTTTGATATCCGAACATTAATTCGTGGCATTATCAATGCTGCAATTGGACAAAAATGTGAGGATGAAAGTTTGGACCTTATGAAAAAAAGGTTGCAAAATACTTTTAGAGATAGAAAGTTTTTGTTAGTGTTGGATGATGTCTGGGATACGGAGTTGATTGGAATAACGATTGAAAAATGGAATGATCTAAAAGGCTTGTTAAATGTGGGAGCTAATGGAAGTAAAATCATCATAACAACACGACATGGATCAGTTGCTTCACTTGTGAATCCCGTATACATGCACCCCTTAGAAGGTCTTCCCCACAAAGACTGCATCTCCTTGTTCATCAAAAGGGCATTTAAAAGAGGAGGAGAGGAGCAGCGCTATCAACATTTGATAGAAATTGGTGAGGATATTGTGAAGAAGTGTGGAGGAGTTCCTTTAGCAGTAGCTACTGTAGGGAGCATGCTCTATTTGAATAGAGAGCGACACCATTGGTTGTGTGTCAGAGATGATGATATGTGGAGTATAGGAAATGACAATATTTTATCTGCACTCAAATTGAGCTACGATGCATTGCCACGACACTTGAAACCATGTTTTGCATTTTGTTCACTTTTCCCAAAGGACTATGAATTCAATAGTGAAATGTTGATTCCATTGTGGATGGCACAAGGCTACCTCAAGACATCTAAGAAAAATGAAGATTTGGATCAGATGGGTATAGACTATATAAGGCAGTGTTGCTCTAGGTCTTTGTTTCAAGTTGATATGGATCTCAAAACATTTATAAGTTTTAAAATACATGATCTACTACATGATTTAGCGATTTTGGTGGCACAAGTAGAGTACTCCACAGTCAATTTCCGTCCTTCTAGTGCTTTTGAAATGGTTCGACATATGTCAATATCCAAAAATGACTTGCTTGGAGAAGAGGCACAAGTTCCTGATTTCATGCTCAAGTCAAAGAAATTGCGAACCATTCGAATTACTAGAGATGGCGAGATGAATCAACATTTTGTGAAGACATGCATCTCGAGGTTCAAATATATGCGGGTGTTAGATATCGGTGGGTCGCCTCTCGAGGAGCTACCAAGTTCCATTGGTAGTTTGTTTCATTTGAGATTCCTCGACTTGTCTGGTAATAGGAATATAAAAAGGCTTCCCAATTCCATCAGTAAGCTACTGAATATGGAGACCTTGTCCCTTGAGAATTGCGACGCACTTGAAGAGATACCCAAGGACATAGGGAACCTGATTAACCTACGAAGCTTGACGATAACAACCCAACAGACCTATTTGCCAAAAGGAATTAGACGCCTCACCTCACTTCAATCTTTAAGTTTTGTTGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGTAAACTGGCGATTTGCAGTTGTGATAAGTTGGAATCCTTGCCACCAAATATGAAACACATGACTGCTTTAGATACTTTGGGTGTCGGTGATTGCGAGAAGCTTCAGTTGATGAGATCAGGGGAAGGTCCTCGAGGTCTTCGATCATTGATTATCGAGAATTCAAGTTTGGAGGCTTTGCCCCCTTGGCTCATTGAAGATTCTGCAGACACTCTACAGAGCATATATCTTGGTGGATGTCATAATCTCACGGCACTACCGGAGTTGATAAAGTTCACATTCCTCGAGGAACTACACATTGAAGTATGCTCCAAATTGTCGGCTTTGCTGCCGCAAGGTTTGCATTGCCTTACTGAGTTGAGAGAATTGAAGATTGAAGGGTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGGTCAAGATTACACTCGACGACTCTGATGATGAAGCTGACAATCGCTTGGCCGACGGTAGGATCGCGTACCTCACTTTGAGACGACGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

882

Amino Acids

100.61

Weight (kDa)

6.26

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 93 6.7e-19 Rx N-terminal domain
NB-ARC PF00931 178 - 356 1.1e-30 NB-ARC domain
WHD_DRP PF23559 440 - 510 1.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 577 - 776 6.7e-29 Leucine-rich repeat region
LRR_RPS2 PF23247 603 - 735 1.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_R13L1-DRL21 PF25019 746 - 804 9.5e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1493
AbsI CCTCGAGG 2 cut(s) 2238, 2390
AccI GTMKAC 2 cut(s) 276, 996
AccII CGCG 1 cut(s) 2621
AciI CCGC 3 cut(s) 259, 1756, 2438
AclWI GGATC 5 cut(s) 976, 1422, 1485, 2080, 2624
AcoI YGGCCR 1 cut(s) 2604
AcsI RAATTY 3 cut(s) 682, 1337, 2266
AcuI CTGAAG 2 cut(s) 331, 2195
AcyI GRCGYC 2 cut(s) 2020, 2641
AfaI GTAC 4 cut(s) 306, 336, 1553, 2624
AfeI AGCGCT 1 cut(s) 1085
AfiI CCNNNNNNNGG 4 cut(s) 78, 615, 1013, 2613
AhlI ACTAGT 1 cut(s) 133
AjiI CACGTC 1 cut(s) 2552
AjuI GAANNNNNNNTTGG 2 cut(s) 2258, 2290
Alw21I GWGCWC 3 cut(s) 88, 99, 141
Alw26I GTCTC 3 cut(s) 482, 1892, 2629
AlwI GGATC 5 cut(s) 976, 1422, 1485, 2080, 2624
Ama87I CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
Aor51HI AGCGCT 1 cut(s) 1085
AoxI GGCC 1 cut(s) 2604
ApeKI GCWGC 5 cut(s) 15, 758, 1081, 2434, 2508
ApoI RAATTY 3 cut(s) 682, 1337, 2266
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 1017, 1593
AspLEI GCGC 2 cut(s) 460, 1086
AspS9I GGNCC 2 cut(s) 793, 2234
AsuHPI GGTGA 5 cut(s) 91, 1121, 2017, 2207, 2540
AsuII TTCGAA 1 cut(s) 1687
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
AvaII GGWCC 2 cut(s) 793, 2234
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 3 cut(s) 1010, 1733, 2237
Bbv12I GWGCWC 3 cut(s) 88, 99, 141
BbvI GCAGC 5 cut(s) 27, 745, 1093, 2421, 2495
BccI CCATC 9 cut(s) 134, 564, 617, 664, 1363, 1414, 1694, 1883, 2539
BceAI ACGGC 2 cut(s) 2373, 2628
BciVI GTATCC 1 cut(s) 859
BcoDI GTCTC 3 cut(s) 482, 1892, 2629
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 7 cut(s) 44, 134, 242, 582, 1452, 1578, 1695
BfmI CTRYAG 4 cut(s) 510, 1158, 2310, 2321
BfoI RGCGCY 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 2293
BglII AGATCT 1 cut(s) 66
BisI GCNGC 6 cut(s) 16, 759, 1082, 2435, 2438, 2509
BlsI GCNGC 6 cut(s) 17, 760, 1083, 2436, 2439, 2510
BmcAI AGTACT 2 cut(s) 336, 1553
Bme18I GGWCC 2 cut(s) 793, 2234
BmeT110I CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
BmgBI CACGTC 1 cut(s) 2552
BmgT120I GGNCC 2 cut(s) 793, 2234
BmiI GGNNCC 1 cut(s) 1955
BmsI GCATC 5 cut(s) 136, 459, 1044, 1264, 1743
BmtI GCTAGC 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 1603
BpiI GAAGAC 3 cut(s) 1010, 1733, 2237
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 1687
BpuEI CTTGAG 4 cut(s) 113, 1370, 1646, 1931
BsaBI GATNNNNATC 2 cut(s) 1413, 1476
BsaHI GRCGYC 2 cut(s) 2020, 2641
BsaI GGTCTC 1 cut(s) 1892
BsaJI CCNNGG 5 cut(s) 72, 463, 613, 1941, 2291
BsaWI WCCGGW 1 cut(s) 2365
BsaXI ACNNNNNCTCC 4 cut(s) 1022, 1052, 1889, 1919
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 615, 1013, 2613
Bse1I ACTGG 4 cut(s) 332, 390, 507, 2118
Bse3DI GCAATG 3 cut(s) 18, 1277, 2449
Bse8I GATNNNNATC 2 cut(s) 1413, 1476
BseDI CCNNGG 5 cut(s) 72, 463, 613, 1941, 2291
BseGI GGATG 9 cut(s) 151, 474, 609, 675, 787, 859, 1374, 2060, 2348
BseJI GATNNNNATC 2 cut(s) 1413, 1476
BseLI CCNNNNNNNGG 4 cut(s) 78, 615, 1013, 2613
BseMI GCAATG 3 cut(s) 18, 1277, 2449
BseMII CTCAG 1 cut(s) 2451
BseNI ACTGG 4 cut(s) 332, 390, 507, 2118
BseRI GAGGAG 8 cut(s) 602, 692, 1083, 1086, 1091, 1149, 1802, 2546
BseXI GCAGC 5 cut(s) 27, 745, 1093, 2421, 2495
BsgI GTGCAG 1 cut(s) 1239
Bsh1236I CGCG 1 cut(s) 2621
BshFI GGCC 1 cut(s) 2606
BsiHKAI GWGCWC 3 cut(s) 88, 99, 141
BsiHKCI CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
BsiSI CCGG 1 cut(s) 2366
BslFI GGGAC 2 cut(s) 369, 1891
BslI CCNNNNNNNGG 4 cut(s) 78, 615, 1013, 2613
BsmAI GTCTC 3 cut(s) 482, 1892, 2629
BsmBI CGTCTC 1 cut(s) 2629
BsmFI GGGAC 2 cut(s) 369, 1891
BsnI GGCC 1 cut(s) 2606
Bso31I GGTCTC 1 cut(s) 1892
BsoBI CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
Bsp119I TTCGAA 1 cut(s) 1687
Bsp1286I GDGCHC 3 cut(s) 88, 99, 141
BspACI CCGC 3 cut(s) 259, 1756, 2438
BspANI GGCC 1 cut(s) 2606
BspCNI CTCAG 1 cut(s) 2452
BspFNI CGCG 1 cut(s) 2621
BspLI GGNNCC 1 cut(s) 1955
BspMAI CTGCAG 1 cut(s) 2314
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 5 cut(s) 976, 1422, 1485, 2080, 2624
BspT104I TTCGAA 1 cut(s) 1687
BspTNI GGTCTC 1 cut(s) 1892
BsrDI GCAATG 3 cut(s) 18, 1277, 2449
BsrI ACTGG 4 cut(s) 332, 390, 507, 2118
BssECI CCNNGG 5 cut(s) 72, 463, 613, 1941, 2291
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 2 cut(s) 2020, 2641
BssT1I CCWWGG 5 cut(s) 72, 463, 613, 1941, 2291
Bst1107I GTATAC 1 cut(s) 997
Bst4CI ACNGT 6 cut(s) 284, 339, 572, 1159, 1561, 2612
Bst6I CTCTTC 3 cut(s) 1629, 1926, 2076
BstACI GRCGYC 2 cut(s) 2020, 2641
BstAPI GCANNNNNTGC 3 cut(s) 83, 2446, 2508
BstBI TTCGAA 1 cut(s) 1687
BstC8I GCNNGC 3 cut(s) 13, 45, 1169
BstDEI CTNAG 3 cut(s) 1009, 1395, 2460
BstF5I GGATG 9 cut(s) 151, 474, 609, 675, 787, 859, 1374, 2060, 2348
BstFNI CGCG 1 cut(s) 2621
BstH2I RGCGCY 1 cut(s) 1087
BstHHI GCGC 2 cut(s) 460, 1086
BstMAI GTCTC 3 cut(s) 482, 1892, 2629
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2446, 2508
BstNSI RCATGY 4 cut(s) 56, 1003, 1171, 1734
BstPAI GACNNNNGTC 1 cut(s) 1603
BstSFI CTRYAG 4 cut(s) 510, 1158, 2310, 2321
BstUI CGCG 1 cut(s) 2621
BstV1I GCAGC 5 cut(s) 27, 745, 1093, 2421, 2495
BstV2I GAAGAC 3 cut(s) 1010, 1733, 2237
BstX2I RGATCY 3 cut(s) 66, 1477, 2085
BstYI RGATCY 3 cut(s) 66, 1477, 2085
BstZ17I GTATAC 1 cut(s) 997
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 1 cut(s) 2606
BtrI CACGTC 1 cut(s) 2552
BtsCI GGATG 9 cut(s) 151, 474, 609, 675, 787, 859, 1374, 2060, 2348
BtsI GCAGTG 1 cut(s) 1448
BtsIMutI CAGTG 2 cut(s) 577, 1448
Cac8I GCNNGC 3 cut(s) 13, 45, 1169
CfoI GCGC 2 cut(s) 460, 1086
Cfr13I GGNCC 2 cut(s) 793, 2234
CseI GACGC 2 cut(s) 1931, 2028
Csp6I GTAC 4 cut(s) 305, 335, 1552, 2623
CspCI CAANNNNNGTGG 2 cut(s) 1272, 1307
CviQI GTAC 4 cut(s) 305, 335, 1552, 2623
DdeI CTNAG 3 cut(s) 1009, 1395, 2460
DraI TTTAAA 2 cut(s) 1063, 1501
EaeI YGGCCR 1 cut(s) 2604
Eam1104I CTCTTC 3 cut(s) 1629, 1926, 2076
EarI CTCTTC 3 cut(s) 1629, 1926, 2076
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 5 cut(s) 72, 463, 613, 1941, 2291
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 1892
Eco32I GATATC 3 cut(s) 577, 727, 1768
Eco47I GGWCC 2 cut(s) 793, 2234
Eco47III AGCGCT 1 cut(s) 1085
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 331, 2195
Eco88I CYCGRG 5 cut(s) 140, 1738, 1784, 2238, 2390
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2234
EcoRI GAATTC 2 cut(s) 1337, 2266
EcoRV GATATC 3 cut(s) 577, 727, 1768
EcoT14I CCWWGG 5 cut(s) 72, 463, 613, 1941, 2291
EcoT22I ATGCAT 2 cut(s) 1279, 1736
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 5 cut(s) 72, 463, 613, 1941, 2291
Esp3I CGTCTC 1 cut(s) 2629
FaqI GGGAC 2 cut(s) 369, 1891
FauI CCCGC 1 cut(s) 1749
FauNDI CATATG 1 cut(s) 1602
FblI GTMKAC 2 cut(s) 276, 996
Fnu4HI GCNGC 6 cut(s) 16, 759, 1082, 2435, 2438, 2509
FokI GGATG 9 cut(s) 158, 481, 596, 682, 794, 866, 1381, 2067, 2355
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 6 cut(s) 16, 759, 1082, 2435, 2438, 2509
FspBI CTAG 7 cut(s) 44, 134, 242, 582, 1452, 1578, 1695
GlaI GCGC 2 cut(s) 459, 1085
GluI GCNGC 6 cut(s) 16, 759, 1082, 2435, 2438, 2509
GsuI CTGGAG 1 cut(s) 524
HaeII RGCGCY 1 cut(s) 1087
HaeIII GGCC 1 cut(s) 2606
HapII CCGG 1 cut(s) 2366
HgaI GACGC 2 cut(s) 1931, 2028
HhaI GCGC 2 cut(s) 460, 1086
Hin1I GRCGYC 2 cut(s) 2020, 2641
Hin6I GCGC 2 cut(s) 458, 1084
HinP1I GCGC 2 cut(s) 458, 1084
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 1972, 2207
HinfI GANTC 9 cut(s) 328, 494, 988, 1357, 1711, 1827, 2141, 2306, 2576
HpaII CCGG 1 cut(s) 2366
HphI GGTGA 5 cut(s) 91, 1121, 2017, 2207, 2540
Hpy166II GTNNAC 5 cut(s) 277, 997, 1316, 2111, 2382
Hpy188I TCNGA 6 cut(s) 350, 715, 731, 1211, 1419, 2581
Hpy188III TCNNGA 7 cut(s) 1387, 1649, 1738, 1784, 2263, 2492, 2560
Hpy8I GTNNAC 5 cut(s) 277, 997, 1316, 2111, 2382
Hpy99I CGWCG 4 cut(s) 1925, 2576, 2612, 2643
HpyAV CCTTC 5 cut(s) 1007, 1584, 2114, 2225, 2477
HpyCH4III ACNGT 6 cut(s) 284, 339, 572, 1159, 1561, 2612
HpyCH4IV ACGT 3 cut(s) 30, 219, 2551
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2446, 2508
HpyF3I CTNAG 3 cut(s) 1009, 1395, 2460
HpySE526I ACGT 3 cut(s) 30, 219, 2551
Hsp92I GRCGYC 2 cut(s) 2020, 2641
HspAI GCGC 2 cut(s) 458, 1084
LmnI GCTCC 5 cut(s) 929, 1078, 1164, 1789, 2420
Lsp1109I GCAGC 5 cut(s) 27, 745, 1093, 2421, 2495
LweI GCATC 5 cut(s) 136, 459, 1044, 1264, 1743
MaeI CTAG 7 cut(s) 44, 134, 242, 582, 1452, 1578, 1695
MaeII ACGT 3 cut(s) 30, 219, 2551
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 66, 1477, 2085
MhlI GDGCHC 3 cut(s) 88, 99, 141
MlyI GAGTC 2 cut(s) 488, 2570
MmeI TCCRAC 4 cut(s) 305, 831, 2032, 2118
Mph1103I ATGCAT 2 cut(s) 1279, 1736
MroXI GAANNNNTTC 2 cut(s) 1017, 1593
MslI CAYNNNNRTG 1 cut(s) 477
MspI CCGG 1 cut(s) 2366
MunI CAATTG 1 cut(s) 762
MvnI CGCG 1 cut(s) 2621
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 103, 2293, 2446, 2508
NdeI CATATG 1 cut(s) 1602
NheI GCTAGC 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 1955
NsiI ATGCAT 2 cut(s) 1279, 1736
NspI RCATGY 4 cut(s) 56, 1003, 1171, 1734
NspV TTCGAA 1 cut(s) 1687
PaeI GCATGC 1 cut(s) 1171
PaeR7I CTCGAG 5 cut(s) 140, 1738, 1784, 2238, 2390
PcsI WCGNNNNNNNCGW 2 cut(s) 2199, 2245
PdmI GAANNNNTTC 2 cut(s) 1017, 1593
PfeI GAWTC 7 cut(s) 328, 988, 1357, 1711, 1827, 2141, 2306
PflFI GACNNNGTC 1 cut(s) 1903
PkrI GCNGC 6 cut(s) 17, 760, 1083, 2436, 2439, 2510
PleI GAGTC 2 cut(s) 488, 2570
PpsI GAGTC 2 cut(s) 488, 2570
PpuMI RGGWCCY 1 cut(s) 2234
PshAI GACNNNNGTC 1 cut(s) 1603
PshBI ATTAAT 1 cut(s) 737
PsiI TTATAA 1 cut(s) 1493
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 2234
PspN4I GGNNCC 1 cut(s) 1955
PspPI GGNCC 2 cut(s) 793, 2234
PspPPI RGGWCCY 1 cut(s) 2234
PspXI VCTCGAGB 3 cut(s) 140, 2238, 2390
PstI CTGCAG 1 cut(s) 2314
PsuI RGATCY 3 cut(s) 66, 1477, 2085
PsyI GACNNNGTC 1 cut(s) 1903
RsaI GTAC 4 cut(s) 306, 336, 1553, 2624
RsaNI GTAC 4 cut(s) 305, 335, 1552, 2623
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 6 cut(s) 16, 759, 1082, 2435, 2438, 2509
Sau96I GGNCC 2 cut(s) 793, 2234
ScaI AGTACT 2 cut(s) 336, 1553
SchI GAGTC 2 cut(s) 488, 2570
SduI GDGCHC 3 cut(s) 88, 99, 141
SfaNI GCATC 5 cut(s) 136, 459, 1044, 1264, 1743
SfcI CTRYAG 4 cut(s) 510, 1158, 2310, 2321
Sfr274I CTCGAG 5 cut(s) 140, 1738, 1784, 2238, 2390
SfuI TTCGAA 1 cut(s) 1687
SinI GGWCC 2 cut(s) 793, 2234
SlaI CTCGAG 5 cut(s) 140, 1738, 1784, 2238, 2390
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 9 cut(s) 92, 140, 1385, 1661, 1738, 1784, 1910, 2238, 2390
SmoI CTYRAG 9 cut(s) 92, 140, 1385, 1661, 1738, 1784, 1910, 2238, 2390
SpeI ACTAGT 1 cut(s) 133
SphI GCATGC 1 cut(s) 1171
SsiI CCGC 3 cut(s) 259, 1756, 2438
SspI AATATT 1 cut(s) 1246
SspMI CTAG 7 cut(s) 44, 134, 242, 582, 1452, 1578, 1695
SstI GAGCTC 1 cut(s) 99
StyI CCWWGG 5 cut(s) 72, 463, 613, 1941, 2291
TaaI ACNGT 6 cut(s) 284, 339, 572, 1159, 1561, 2612
TaiI ACGT 3 cut(s) 33, 222, 2554
TatI WGTACW 2 cut(s) 334, 1551
TauI GCSGC 1 cut(s) 2440
TfiI GAWTC 7 cut(s) 328, 988, 1357, 1711, 1827, 2141, 2306
TscAI CASTG 2 cut(s) 577, 1448
TseI GCWGC 5 cut(s) 15, 758, 1081, 2434, 2508
TspGWI ACGGA 2 cut(s) 884, 1559
TspRI CASTG 2 cut(s) 577, 1448
Tth111I GACNNNGTC 1 cut(s) 1903
VpaK11BI GGWCC 2 cut(s) 793, 2234
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 3 cut(s) 682, 1337, 2266
XceI RCATGY 4 cut(s) 56, 1003, 1171, 1734
XhoI CTCGAG 5 cut(s) 140, 1738, 1784, 2238, 2390
XmiI GTMKAC 2 cut(s) 276, 996
XmnI GAANNNNTTC 2 cut(s) 1017, 1593
XspI CTAG 7 cut(s) 44, 134, 242, 582, 1452, 1578, 1695
ZrmI AGTACT 2 cut(s) 336, 1553
Zsp2I ATGCAT 2 cut(s) 1279, 1736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.