Prupe.5G111200_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000159
Physical Location & Seq
Forward (+)
11545020 .. 11548482
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G111200.1

Sequence Viewer

Length: 2607 bp
ATGGAGTCATTTTCCTTCAACGTTGTTGAGAAAGTGTTGGAAAGGCTTGCTTCCCATGCTTACCACGAAATTCGTTTGGCTTTGGATGTCAAGGATGATCTAATCAAGCTCCAGGAAACCTTATCAACCATCGCAAAAGTGGTCCTAGATGCAGAGGAAAAACAGAGGAAGGATCCCCTGCTTGCTGACTGGTTAGGGAAGCTAAAAGATGTTTGCTATGATATGGATGATGTTCTCGACCAATTCGAATTCCGAAAATTGAGGATGCAAGTGCTCAACTCTCGGAGTAATGTCAAAGGAAAGGTACGCAACTTCTTTTCGCGTCCAAATTCCTTTATGTTAAGCTACAAAATTGGTCAGAGAATCAAAGATATTAGAGAAAGATTGGGTGAAATTGCAGCTGCAAAAGCTCAGTTCAATCTGTATGAACGAGCTGCAGATTGGCAAGGGATGCACATAGAGAGGGAGACCCACTCTTTTGTGCATGCCCCGGATGTGATTGGAAGAGATAAAGATAAAGAAGAGATGATCATGCATCTTCTTAATGACAATCGCACTGGTCATGGGGAGGAGAATGTTTCTGTTATTTCCATTAATGGGTTAGGAGGTCTGGGAAAGACAACACTTGCTAAACTGGTGTATAATGATAACAGAGTAGTAGGGAACTTTGAGTTGAGAATATGGGTGTGCGTCTCAGATGATTTCGACAATAAAAGATTGCTTCGAGAAATTGTTACTGCTGCAACAAGCCAAAAATGTGTGGATGAAAGCATTGAACAGATGCAAATTAAGCTGCGGCATGCTTTGACATGTAAGAAACTTCTGTTAGTGTTGGATGATGTTTGGGACAAGGGGCCAATGGGAATCACAATGAAGAAATGGATTGATTTGAAGTCTTTGTTAAATGTTGTAGCCAATGGGAGTAAAATCATTGTAACAACACGCAATGAATCGGTTGCATTGCTTATGGGGCATGCACACATGCATCTACTGAAGGGCCTTCCCCACTCAGATTGCATGACTATATTCATAAAAGTGGCATTTACAAAAAGAGAACAGGGAGACTATCCCAAGTTAATAAAAATTGGGGAAGATATTGTGAGGAAGTGTGGAGGGGTTCCTCTTGCCTTGTACACCCTAGGGGGCCTGCTCTATTCGAACAAGGATGAGCGATACTGGTCACATGTTAGAGACAGTGACATATGGAAATTGGAACAGGGAAGCGACGACATTTTACCTGCGCTTAAGTTAAGCTACGATGCATTGCCGATCTACTTGAAACCTTGTTTTGCCTTTTGTTCACTTTATCCAAAGGACTATGTATTTAGAAGCGCAGAATTGATCCCGTTGTTGATGGCTGAAGGCTTCATTCAATCCTCCAAAGGTAACGGGAACCAAGAGCTTGAAGATATTGGTCTGGACTACATAAGGCAACTATGTTCTAGGTATTTCTTTCAGATTGAGGAAGATGATTTCCTTTTTCTACGGTTTAGAATGCATGACCTTGTTCATGATTTAGCAATCTCAATGGCAAGGGTAGAGTATTCGTCTCTAAATTTTCGTCCCTCTGATTCATCTAAGATGGTTCGGCATGTGTCAATATCTCAGAAGGACTTGTCCAAGGAAAATGAAGAAAACCCCAAATTCTTACTTCGTCTGGAGAAATTGAGAACCATTCTAATTCCAGATTTGGACAGTGAATATGTTCCTATAAAAGTTGGGATCAACAGTCAATCTTTTTTAAAGAAATGCATCTCAAGAATCAACTACCTACGAGTGCTAGATCTTAGTAACTTGACTCTTAAGGTGCTGCCATGTTCCATCGGTAACTTGAGTCACTTGAGGTATCTCGATGTGTCATATAATCAGCATATAAATAAGCTTCCTGATTCCATTTGCAAGCTGCATCATTTACAATCCTTGCTACTTATAAACTGTGGAAAACTCAAGGAGTTGCCTAAAGACATGGGAAATCTGATTAGCCTTAGGTACTTGGCCTTAACCATAAAGAAGACGCATTTGCCAGAAGCAATTGGTCGTCTCACCTCTCTTCGAACTTTATGCGTCAGTGCATGCAAAAATCTTAAATCTTTGGGCAAAGAGATGCGATCCCTCACTAACCTTCGAATGCTAGTCATTGTGAGCTGTCAAAACTTGGAATCCTTGTCATCTTGCAACATGACCACATTGGAAACTTTAGTCATCTATAGTTGCCCAAAGCTAAATCTGATGGGGTCAGAAGAAGGCATTCGAGGTCTTCAATCGTTTTGGATTGTAGGTTCGAATTTGACAGCCTTGCCTCATTGGTTGCAAGAGTCTGCAAACACACTAAAAACATTACAGTTGGGATTTTGTACCTATCTACAGTCACTTCCGGAATGGTTTCAAAACTTTCCTTCGCTTCAAAAACTAAAGATTACAAATTGTTCCCATTTGTTTGCTTTGCCCGAGGGCATGGATCGCCTTACTGCATTGAGAGAGTTGGAGATTAGCAATTGTCCTTTAGTTAGGAGATGCAGGTATGAAGGTGAGGATTGGTCCAAGATCGCTCATGTCCCAAAGATCATACTCAATGGTCATCGACGGCTAATTAGATCTCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

869

Amino Acids

99.38

Weight (kDa)

8.72

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1931
Acc36I ACCTGC 2 cut(s) 1246, 2508
AccII CGCG 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 2374
AciI CCGC 1 cut(s) 796
AclI AACGTT 1 cut(s) 21
AclWI GGATC 6 cut(s) 167, 180, 1336, 1730, 2103, 2466
AcsI RAATTY 6 cut(s) 69, 248, 328, 1555, 1643, 2284
AcuI CTGAAG 2 cut(s) 1013, 1380
AfaI GTAC 4 cut(s) 306, 1133, 1991, 2356
AfiI CCNNNNNNNGG 1 cut(s) 597
AflII CTTAAG 2 cut(s) 1244, 1802
AflIII ACRYGT 2 cut(s) 809, 1183
AjnI CCWGG 1 cut(s) 111
AloI GAACNNNNNNTCC 2 cut(s) 2263, 2295
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 6 cut(s) 461, 697, 1056, 1185, 1554, 2045
AlwI GGATC 6 cut(s) 167, 180, 1336, 1730, 2103, 2466
Ama87I CYCGRG 1 cut(s) 2447
Aor13HI TCCGGA 1 cut(s) 2374
AoxI GGCC 4 cut(s) 854, 997, 1144, 1995
ApeKI GCWGC 7 cut(s) 398, 401, 434, 740, 793, 1810, 1903
ApoI RAATTY 6 cut(s) 69, 248, 328, 1555, 1643, 2284
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 3 cut(s) 1704, 2247, 2382
AspA2I CCTAGG 1 cut(s) 1138
AspLEI GCGC 2 cut(s) 1243, 1334
AspS9I GGNCC 5 cut(s) 142, 854, 997, 1144, 2538
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 401, 2035, 2540
AsuII TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
AvaI CYCGRG 1 cut(s) 2447
AvaII GGWCC 2 cut(s) 142, 2538
AvrII CCTAGG 1 cut(s) 1138
AxyI CCTNAGG 1 cut(s) 1985
BamHI GGATCC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 2018, 2249
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 7 cut(s) 388, 410, 421, 727, 780, 1797, 1890
BccI CCATC 5 cut(s) 137, 1348, 1576, 1829, 2224
BceAI ACGGC 1 cut(s) 2600
BcgI CGANNNNNNTGC 2 cut(s) 2043, 2077
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 6 cut(s) 461, 697, 1056, 1185, 1554, 2045
BfaI CTAG 5 cut(s) 146, 1139, 1443, 1781, 2132
BfmI CTRYAG 3 cut(s) 435, 2206, 2363
BfrI CTTAAG 2 cut(s) 1244, 1802
BfuAI ACCTGC 2 cut(s) 1246, 2508
BglII AGATCT 2 cut(s) 1783, 2594
BisI GCNGC 8 cut(s) 399, 402, 435, 741, 794, 797, 1811, 1904
BlnI CCTAGG 1 cut(s) 1138
BlsI GCNGC 8 cut(s) 400, 403, 436, 742, 795, 798, 1812, 1905
Bme1390I CCNGG 2 cut(s) 113, 491
Bme18I GGWCC 2 cut(s) 142, 2538
BmeT110I CYCGRG 1 cut(s) 2447
BmgT120I GGNCC 5 cut(s) 142, 854, 997, 1144, 2538
BmiI GGNNCC 5 cut(s) 174, 855, 1119, 1145, 1394
BmrFI CCNGG 2 cut(s) 113, 491
BpiI GAAGAC 2 cut(s) 2018, 2249
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 2 cut(s) 95, 1679
Bpu14I TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
BpuEI CTTGAG 4 cut(s) 1741, 1852, 1861, 1931
BpuMI CCSGG 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 4 cut(s) 489, 1138, 1620, 2448
BsaWI WCCGGW 1 cut(s) 2374
Bsc4I CCNNNNNNNGG 1 cut(s) 597
Bse1I ACTGG 4 cut(s) 194, 562, 639, 1181
Bse21I CCTNAGG 1 cut(s) 1985
Bse3DI GCAATG 3 cut(s) 952, 959, 1262
BseAI TCCGGA 1 cut(s) 2374
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 4 cut(s) 489, 1138, 1620, 2448
BseGI GGATG 9 cut(s) 91, 100, 232, 270, 456, 499, 769, 841, 1171
BseLI CCNNNNNNNGG 1 cut(s) 597
BseMI GCAATG 3 cut(s) 952, 959, 1262
BseMII CTCAG 4 cut(s) 425, 708, 1023, 1619
BseNI ACTGG 4 cut(s) 194, 562, 639, 1181
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 7 cut(s) 388, 410, 421, 727, 780, 1797, 1890
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 4 cut(s) 856, 999, 1146, 1997
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 2447
BsiSI CCGG 2 cut(s) 491, 2375
BslFI GGGAC 3 cut(s) 860, 1548, 2540
BslI CCNNNNNNNGG 1 cut(s) 597
BsmAI GTCTC 6 cut(s) 461, 697, 1056, 1185, 1554, 2045
BsmBI CGTCTC 3 cut(s) 697, 1554, 2045
BsmFI GGGAC 3 cut(s) 860, 1548, 2540
BsmI GAATGC 3 cut(s) 1500, 2133, 2247
BsnI GGCC 4 cut(s) 856, 999, 1146, 1997
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 2447
Bsp119I TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
Bsp1286I GDGCHC 1 cut(s) 276
Bsp13I TCCGGA 1 cut(s) 2374
Bsp1407I TGTACA 1 cut(s) 1131
BspACI CCGC 1 cut(s) 796
BspANI GGCC 4 cut(s) 856, 999, 1146, 1997
BspCNI CTCAG 4 cut(s) 424, 707, 1022, 1618
BspEI TCCGGA 1 cut(s) 2374
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 1510
BspLI GGNNCC 5 cut(s) 174, 855, 1119, 1145, 1394
BspMAI CTGCAG 1 cut(s) 439
BspMI ACCTGC 2 cut(s) 1246, 2508
BspPI GGATC 6 cut(s) 167, 180, 1336, 1730, 2103, 2466
BspT104I TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
BspTI CTTAAG 2 cut(s) 1244, 1802
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 3 cut(s) 952, 959, 1262
BsrGI TGTACA 1 cut(s) 1131
BsrI ACTGG 4 cut(s) 194, 562, 639, 1181
BssECI CCNNGG 4 cut(s) 489, 1138, 1620, 2448
BssT1I CCWWGG 2 cut(s) 1138, 1620
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 7 cut(s) 1196, 1488, 1697, 1730, 1937, 2343, 2367
Bst6I CTCTTC 3 cut(s) 499, 516, 2055
BstAFI CTTAAG 2 cut(s) 1244, 1802
BstAPI GCANNNNNTGC 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 1131
BstBI TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
BstC8I GCNNGC 8 cut(s) 48, 183, 486, 801, 975, 1148, 1901, 2074
BstDEI CTNAG 7 cut(s) 411, 694, 1009, 1578, 1605, 1787, 1985
BstF5I GGATG 9 cut(s) 91, 100, 232, 270, 456, 499, 769, 841, 1171
BstFNI CGCG 1 cut(s) 322
BstHHI GCGC 2 cut(s) 1243, 1334
BstMAI GTCTC 6 cut(s) 461, 697, 1056, 1185, 1554, 2045
BstMWI GCNNNNNNNGC 6 cut(s) 56, 407, 451, 790, 970, 2460
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 8 cut(s) 488, 803, 813, 977, 985, 1187, 1595, 2076
BstSCI CCNGG 2 cut(s) 111, 489
BstSFI CTRYAG 3 cut(s) 435, 2206, 2363
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 7 cut(s) 388, 410, 421, 727, 780, 1797, 1890
BstV2I GAAGAC 2 cut(s) 2018, 2249
BstX2I RGATCY 3 cut(s) 172, 1783, 2594
BstYI RGATCY 3 cut(s) 172, 1783, 2594
Bsu36I CCTNAGG 1 cut(s) 1985
BsuRI GGCC 4 cut(s) 856, 999, 1146, 1997
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 9 cut(s) 91, 100, 232, 270, 456, 499, 769, 841, 1171
BtsIMutI CAGTG 4 cut(s) 555, 1201, 1702, 2074
BveI ACCTGC 2 cut(s) 1246, 2508
Cac8I GCNNGC 8 cut(s) 48, 183, 486, 801, 975, 1148, 1901, 2074
CciI TCATGA 1 cut(s) 1510
CfoI GCGC 2 cut(s) 1243, 1334
Cfr13I GGNCC 5 cut(s) 142, 854, 997, 1144, 2538
CseI GACGC 4 cut(s) 311, 679, 2023, 2053
Csp6I GTAC 4 cut(s) 305, 1132, 1990, 2355
CspCI CAANNNNNGTGG 2 cut(s) 995, 1030
CviQI GTAC 4 cut(s) 305, 1132, 1990, 2355
DdeI CTNAG 7 cut(s) 411, 694, 1009, 1578, 1605, 1787, 1985
DraI TTTAAA 1 cut(s) 1743
Eam1104I CTCTTC 3 cut(s) 499, 516, 2055
EarI CTCTTC 3 cut(s) 499, 516, 2055
Eco130I CCWWGG 2 cut(s) 1138, 1620
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 2 cut(s) 142, 2538
Eco57I CTGAAG 2 cut(s) 1013, 1380
Eco81I CCTNAGG 1 cut(s) 1985
Eco88I CYCGRG 1 cut(s) 2447
EcoO109I RGGNCCY 2 cut(s) 997, 1144
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 2 cut(s) 1138, 1620
EcoT22I ATGCAT 5 cut(s) 537, 987, 1264, 1500, 1754
ErhI CCWWGG 2 cut(s) 1138, 1620
Esp3I CGTCTC 3 cut(s) 697, 1554, 2045
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 3 cut(s) 860, 1548, 2540
FauNDI CATATG 1 cut(s) 1202
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 8 cut(s) 399, 402, 435, 741, 794, 797, 1811, 1904
FokI GGATG 9 cut(s) 98, 107, 239, 277, 463, 506, 776, 848, 1178
Fsp4HI GCNGC 8 cut(s) 399, 402, 435, 741, 794, 797, 1811, 1904
FspBI CTAG 5 cut(s) 146, 1139, 1443, 1781, 2132
GlaI GCGC 2 cut(s) 1242, 1333
GluI GCNGC 8 cut(s) 399, 402, 435, 741, 794, 797, 1811, 1904
GsuI CTGGAG 2 cut(s) 95, 1679
HaeIII GGCC 4 cut(s) 856, 999, 1146, 1997
HapII CCGG 2 cut(s) 491, 2375
HgaI GACGC 4 cut(s) 311, 679, 2023, 2053
HhaI GCGC 2 cut(s) 1243, 1334
Hin6I GCGC 2 cut(s) 1241, 1332
HinP1I GCGC 2 cut(s) 1241, 1332
HindIII AAGCTT 1 cut(s) 1880
HpaII CCGG 2 cut(s) 491, 2375
HphI GGTGA 3 cut(s) 401, 2035, 2540
Hpy166II GTNNAC 2 cut(s) 1134, 1301
Hpy8I GTNNAC 2 cut(s) 1134, 1301
Hpy99I CGWCG 2 cut(s) 1229, 2586
HpyCH4III ACNGT 7 cut(s) 1196, 1488, 1697, 1730, 1937, 2343, 2367
HpyCH4IV ACGT 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 407, 451, 790, 970, 2460
HpyF3I CTNAG 7 cut(s) 411, 694, 1009, 1578, 1605, 1787, 1985
HpySE526I ACGT 1 cut(s) 21
HspAI GCGC 2 cut(s) 1241, 1332
Kpn2I TCCGGA 1 cut(s) 2374
Ksp22I TGATCA 1 cut(s) 528
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 7 cut(s) 388, 410, 421, 727, 780, 1797, 1890
MaeI CTAG 5 cut(s) 146, 1139, 1443, 1781, 2132
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 9 cut(s) 733, 934, 1179, 1196, 1385, 1790, 1826, 1835, 2367
MfeI CAATTG 2 cut(s) 2031, 2494
MflI RGATCY 3 cut(s) 172, 1783, 2594
MhlI GDGCHC 1 cut(s) 276
MlyI GAGTC 4 cut(s) 14, 1792, 1843, 2324
MmeI TCCRAC 3 cut(s) 18, 813, 2463
Mph1103I ATGCAT 5 cut(s) 537, 987, 1264, 1500, 1754
MroI TCCGGA 1 cut(s) 2374
MroXI GAANNNNTTC 3 cut(s) 1704, 2247, 2382
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 401
MspCI CTTAAG 2 cut(s) 1244, 1802
MspI CCGG 2 cut(s) 491, 2375
MspR9I CCNGG 2 cut(s) 113, 491
MunI CAATTG 2 cut(s) 2031, 2494
Mva1269I GAATGC 3 cut(s) 1500, 2133, 2247
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 6 cut(s) 56, 407, 451, 790, 970, 2460
NciI CCSGG 1 cut(s) 491
NdeI CATATG 1 cut(s) 1202
NlaIV GGNNCC 5 cut(s) 174, 855, 1119, 1145, 1394
NmuCI GTSAC 4 cut(s) 1179, 1196, 1835, 2367
NsiI ATGCAT 5 cut(s) 537, 987, 1264, 1500, 1754
NspI RCATGY 8 cut(s) 488, 803, 813, 977, 985, 1187, 1595, 2076
NspV TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
PaeI GCATGC 4 cut(s) 488, 803, 977, 2076
PagI TCATGA 1 cut(s) 1510
PciI ACATGT 2 cut(s) 809, 1183
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 3 cut(s) 1500, 2133, 2247
PdmI GAANNNNTTC 3 cut(s) 1704, 2247, 2382
PfeI GAWTC 7 cut(s) 363, 864, 950, 1571, 1761, 1889, 2159
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 8 cut(s) 400, 403, 436, 742, 795, 798, 1812, 1905
PleI GAGTC 4 cut(s) 13, 1792, 1842, 2323
PpsI GAGTC 4 cut(s) 13, 1792, 1842, 2323
PscI ACATGT 2 cut(s) 809, 1183
PshBI ATTAAT 1 cut(s) 594
PsiI TTATAA 1 cut(s) 1931
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 5 cut(s) 174, 855, 1119, 1145, 1394
PspPI GGNCC 5 cut(s) 142, 854, 997, 1144, 2538
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 3 cut(s) 172, 1783, 2594
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 4 cut(s) 306, 1133, 1991, 2356
RsaNI GTAC 4 cut(s) 305, 1132, 1990, 2355
RseI CAYNNNNRTG 1 cut(s) 1034
SatI GCNGC 8 cut(s) 399, 402, 435, 741, 794, 797, 1811, 1904
Sau96I GGNCC 5 cut(s) 142, 854, 997, 1144, 2538
SchI GAGTC 4 cut(s) 14, 1792, 1843, 2324
ScrFI CCNGG 2 cut(s) 113, 491
SduI GDGCHC 1 cut(s) 276
SfcI CTRYAG 3 cut(s) 435, 2206, 2363
SfuI TTCGAA 5 cut(s) 246, 1157, 2053, 2125, 2282
SinI GGWCC 2 cut(s) 142, 2538
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 6 cut(s) 1244, 1756, 1802, 1831, 1840, 1946
SmoI CTYRAG 6 cut(s) 1244, 1756, 1802, 1831, 1840, 1946
SphI GCATGC 4 cut(s) 488, 803, 977, 2076
SsiI CCGC 1 cut(s) 796
SspMI CTAG 5 cut(s) 146, 1139, 1443, 1781, 2132
StyD4I CCNGG 2 cut(s) 111, 489
StyI CCWWGG 2 cut(s) 1138, 1620
TaaI ACNGT 7 cut(s) 1196, 1488, 1697, 1730, 1937, 2343, 2367
TaiI ACGT 1 cut(s) 24
TatI WGTACW 1 cut(s) 1131
TauI GCSGC 1 cut(s) 799
TfiI GAWTC 7 cut(s) 363, 864, 950, 1571, 1761, 1889, 2159
TscAI CASTG 4 cut(s) 562, 1201, 1702, 2074
TseFI GTSAC 4 cut(s) 1179, 1196, 1835, 2367
TseI GCWGC 7 cut(s) 398, 401, 434, 740, 793, 1810, 1903
Tsp45I GTSAC 4 cut(s) 1179, 1196, 1835, 2367
TspRI CASTG 4 cut(s) 562, 1201, 1702, 2074
Vha464I CTTAAG 2 cut(s) 1244, 1802
VpaK11BI GGWCC 2 cut(s) 142, 2538
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 6 cut(s) 69, 248, 328, 1555, 1643, 2284
XceI RCATGY 8 cut(s) 488, 803, 813, 977, 985, 1187, 1595, 2076
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmaJI CCTAGG 1 cut(s) 1138
XmnI GAANNNNTTC 3 cut(s) 1704, 2247, 2382
XspI CTAG 5 cut(s) 146, 1139, 1443, 1781, 2132
Zsp2I ATGCAT 5 cut(s) 537, 987, 1264, 1500, 1754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.