Rroxscaffold_2G00126270

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000159
Physical Location & Seq
Forward (+)
61429547 .. 61433088
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126270.1

Sequence Viewer

Length: 564 bp
ATGGAGTTTGCCAGCAGCATTGCAGATAACGTCTTGCGTAGGCTAGCTTTACATGCTTCCCCGCAGATCTCCTTGTCATGGAATGCCCAACTTGAGCTCACCAAGCTCAACAACACCTTATCTACCATCAAACTAGTGCTCGAGGATGCAGAGAAGAAGCAAGTGAGAAATCCGCTAATCACTCGTTGGTTAGGAAATCTCAAAGATGTTTGTCATGACGTTGATGATGTCTTGGACGAACTGGACTTCCAAAAGTTGCGGTTGAAAGTGGAGGTCGACAACTATGGGAAGATCAAAGGAAAGGTACGCCAATTTTTTTCCCGATGGAATCCAGTAGTGTTTAACTTCAAAATGGGACATAAAGTGAAAGAGATTAGAAAAAGACTAGTTGAAATTGATAATGAACAGAGACAGTTTGCTCTCCTTGAGTTTGCTAAGATAGCTGAAGATCTTCGTGCGCCCCAACAAGTGCGCGATGACAGAAGGATGACTACCTCTACGGTTGAGGTTTCAAATGTTATTGGAAGAGATGATGATAAAAACAGATTATCAAGCATCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

187

Amino Acids

21.73

Weight (kDa)

9.21

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 93 5.3e-18 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 474
AciI CCGC 3 cut(s) 62, 173, 259
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 4 cut(s) 265, 349, 392, 513
AhlI ACTAGT 2 cut(s) 133, 385
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
AluBI AGCT 4 cut(s) 47, 97, 106, 443
AluI AGCT 4 cut(s) 47, 97, 106, 443
Alw21I GWGCWC 2 cut(s) 99, 141
Alw26I GTCTC 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 15
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 2 cut(s) 460, 474
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 140
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 475, 507
Bbv12I GWGCWC 2 cut(s) 99, 141
BbvI GCAGC 1 cut(s) 27
BccI CCATC 2 cut(s) 134, 318
BcoDI GTCTC 1 cut(s) 403
BcuI ACTAGT 2 cut(s) 133, 385
BfaI CTAG 3 cut(s) 44, 134, 386
BglII AGATCT 2 cut(s) 66, 448
BisI GCNGC 1 cut(s) 16
BlsI GCNGC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 140
BmsI GCATC 1 cut(s) 136
BmtI GCTAGC 1 cut(s) 47
BpuEI CTTGAG 2 cut(s) 113, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 246, 332
Bse3DI GCAATG 1 cut(s) 18
BseGI GGATG 2 cut(s) 151, 492
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 246, 332
BseXI GCAGC 1 cut(s) 27
Bsh1236I CGCG 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 99, 141
BsiHKCI CYCGRG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 403
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 99, 141
Bsp143I GATC 3 cut(s) 66, 291, 448
BspACI CCGC 3 cut(s) 62, 173, 259
BspFNI CGCG 1 cut(s) 474
BspHI TCATGA 1 cut(s) 214
BspOI GCTAGC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 246, 332
BssMI GATC 3 cut(s) 66, 291, 448
Bst4CI ACNGT 2 cut(s) 414, 502
Bst6I CTCTTC 1 cut(s) 520
BstC8I GCNNGC 2 cut(s) 13, 45
BstDEI CTNAG 1 cut(s) 435
BstF5I GGATG 2 cut(s) 151, 492
BstFNI CGCG 1 cut(s) 474
BstHHI GCGC 2 cut(s) 460, 474
BstKTI GATC 3 cut(s) 69, 294, 451
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 66, 291, 448
BstMWI GCNNNNNNNGC 3 cut(s) 53, 103, 440
BstNSI RCATGY 1 cut(s) 56
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 27
BstX2I RGATCY 2 cut(s) 66, 448
BstYI RGATCY 2 cut(s) 66, 448
BtgZI GCGATG 1 cut(s) 489
BtsCI GGATG 2 cut(s) 151, 492
Cac8I GCNNGC 2 cut(s) 13, 45
CciI TCATGA 1 cut(s) 214
CfoI GCGC 2 cut(s) 460, 474
Csp6I GTAC 1 cut(s) 305
CviAII CATG 3 cut(s) 53, 78, 215
CviJI RGCY 5 cut(s) 43, 47, 97, 106, 443
CviKI_1 RGCY 5 cut(s) 43, 47, 97, 106, 443
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 1 cut(s) 435
DpnI GATC 3 cut(s) 68, 293, 450
DpnII GATC 3 cut(s) 66, 291, 448
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 3 cut(s) 56, 81, 218
FaiI YATR 5 cut(s) 54, 79, 216, 285, 360
FalI AAGNNNNNCTT 1 cut(s) 544
FaqI GGGAC 1 cut(s) 369
FatI CATG 3 cut(s) 52, 77, 214
FauI CCCGC 1 cut(s) 69
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 16
FokI GGATG 2 cut(s) 158, 499
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 16
FspBI CTAG 3 cut(s) 44, 134, 386
GlaI GCGC 2 cut(s) 459, 473
GluI GCNGC 1 cut(s) 16
HhaI GCGC 2 cut(s) 460, 474
Hin1II CATG 3 cut(s) 56, 81, 218
Hin6I GCGC 2 cut(s) 458, 472
HinP1I GCGC 2 cut(s) 458, 472
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 328
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 277
Hpy188III TCNNGA 3 cut(s) 215, 321, 561
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 1 cut(s) 477
HpyCH4III ACNGT 2 cut(s) 414, 502
HpyCH4IV ACGT 2 cut(s) 30, 219
HpyCH4V TGCA 2 cut(s) 23, 149
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 103, 440
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 2 cut(s) 30, 219
Hsp92II CATG 3 cut(s) 56, 81, 218
HspAI GCGC 2 cut(s) 458, 472
Kzo9I GATC 3 cut(s) 66, 291, 448
LpnPI CCDG 3 cut(s) 25, 227, 345
Lsp1109I GCAGC 1 cut(s) 27
LweI GCATC 1 cut(s) 136
MaeI CTAG 3 cut(s) 44, 134, 386
MaeII ACGT 2 cut(s) 30, 219
MalI GATC 3 cut(s) 68, 293, 450
MboI GATC 3 cut(s) 66, 291, 448
MboII GAAGA 5 cut(s) 166, 301, 443, 458, 537
MflI RGATCY 2 cut(s) 66, 448
MhlI GDGCHC 2 cut(s) 99, 141
MluCI AATT 2 cut(s) 311, 393
MnlI CCTC 4 cut(s) 136, 265, 499, 505
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 88
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 3 cut(s) 53, 103, 440
NdeII GATC 3 cut(s) 66, 291, 448
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 3 cut(s) 56, 81, 218
NspI RCATGY 1 cut(s) 56
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 214
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 99
PspXI VCTCGAGB 1 cut(s) 140
PsuI RGATCY 2 cut(s) 66, 448
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 16
Sau3AI GATC 3 cut(s) 66, 291, 448
SduI GDGCHC 2 cut(s) 99, 141
SfaNI GCATC 1 cut(s) 136
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 3 cut(s) 92, 140, 425
SmoI CTYRAG 3 cut(s) 92, 140, 425
SpeI ACTAGT 2 cut(s) 133, 385
Sse9I AATT 2 cut(s) 311, 393
SsiI CCGC 3 cut(s) 62, 173, 259
SspMI CTAG 3 cut(s) 44, 134, 386
SstI GAGCTC 1 cut(s) 99
TaaI ACNGT 2 cut(s) 414, 502
TaiI ACGT 2 cut(s) 33, 222
TaqI TCGA 2 cut(s) 141, 276
TasI AATT 2 cut(s) 311, 393
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseI GCWGC 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 417
XceI RCATGY 1 cut(s) 56
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 44, 134, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.