Rh5DG066000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000159
Physical Location & Seq
Reverse (-)
5614656 .. 5615112
457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG066000.1

Sequence Viewer

Length: 360 bp
ATGGAGTCTTTTGCCTCAAACAAAGCACAGATCGTCTTCGAAAAGCTAGCTTCCCGTTCTTACGAAGAAATTCACATCGCATTGAGTGTCAAAGTTGAGCTGAACAAGCTTGAGGAGACTTTATCAAGCATCACTTTACTGCTCCTGGATGCGGAGGAAAAGCAGATGAACAACCGCGTACTCACTGAGTGGTTGGGAAAGCTCAAAGATGTTTGCTACGATATTGATGATGAGCTCGATGACTTTGCATTCAAACAGTTGCGGATGCAAGTGCTTGTCCGTGGAAGCGTGGCCGGAAAGCTGCCAAAGATCAGTTTCATCTTAAAGAAAGAGCCGGAGAGTGGCATGGTCTGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

119

Amino Acids

13.55

Weight (kDa)

5.08

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 90 1.8e-19 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 3 cut(s) 152, 175, 262
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 189
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 151, 341
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 7 cut(s) 46, 50, 100, 109, 202, 235, 301
AluI AGCT 7 cut(s) 46, 50, 100, 109, 202, 235, 301
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 1 cut(s) 301
ApoI RAATTY 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 69
AsuII TTCGAA 1 cut(s) 39
AsuNHI GCTAGC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 288
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 47
BisI GCNGC 1 cut(s) 302
BlsI GCNGC 1 cut(s) 303
Bme1390I CCNGG 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 3 cut(s) 138, 139, 255
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 280
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 341
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 154, 270
BseLI CCNNNNNNNGG 2 cut(s) 151, 341
BseMII CTCAG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 288
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 2 cut(s) 294, 335
BslI CCNNNNNNNGG 2 cut(s) 151, 341
BsmAI GTCTC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 30, 309
BspACI CCGC 3 cut(s) 152, 175, 262
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 178
BspFNI CGCG 1 cut(s) 177
BspOI GCTAGC 1 cut(s) 50
BspT104I TTCGAA 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 2 cut(s) 30, 309
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 154, 270
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 2 cut(s) 33, 312
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 30, 309
BstMWI GCNNNNNNNGC 2 cut(s) 106, 351
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 28
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 2 cut(s) 154, 270
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 179
CviAII CATG 2 cut(s) 346, 357
CviJI RGCY 9 cut(s) 46, 50, 100, 109, 202, 235, 293, 301, 334
CviKI_1 RGCY 9 cut(s) 46, 50, 100, 109, 202, 235, 293, 301, 334
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 1 cut(s) 186
DpnI GATC 2 cut(s) 32, 311
DpnII GATC 2 cut(s) 30, 309
DraIII CACNNNGTG 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 2 cut(s) 349, 360
FaiI YATR 2 cut(s) 347, 358
FalI AAGNNNNNCTT 2 cut(s) 118, 150
FatI CATG 2 cut(s) 345, 356
Fnu4HI GCNGC 1 cut(s) 302
FokI GGATG 2 cut(s) 161, 277
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 302
FspBI CTAG 1 cut(s) 47
GluI GCNGC 1 cut(s) 302
HaeIII GGCC 1 cut(s) 293
HapII CCGG 2 cut(s) 294, 335
Hin1II CATG 2 cut(s) 349, 360
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 1 cut(s) 5
HpaII CCGG 2 cut(s) 294, 335
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4V TGCA 3 cut(s) 248, 268, 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 351
HpyF3I CTNAG 1 cut(s) 186
Hsp92II CATG 2 cut(s) 349, 360
Kzo9I GATC 2 cut(s) 30, 309
LmnI GCTCC 1 cut(s) 147
LpnPI CCDG 4 cut(s) 131, 158, 307, 348
Lsp1109I GCAGC 1 cut(s) 288
LweI GCATC 3 cut(s) 138, 139, 255
MaeI CTAG 1 cut(s) 47
MalI GATC 2 cut(s) 32, 311
MboI GATC 2 cut(s) 30, 309
MboII GAAGA 2 cut(s) 28, 77
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 1 cut(s) 69
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 3 cut(s) 25, 106, 148
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 323
MspI CCGG 2 cut(s) 294, 335
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 106, 351
NdeII GATC 2 cut(s) 30, 309
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 2 cut(s) 349, 360
NspV TTCGAA 1 cut(s) 39
PctI GAATGC 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 69
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 1 cut(s) 303
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 1 cut(s) 302
Sau3AI GATC 2 cut(s) 30, 309
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 237
SetI ASST 7 cut(s) 48, 52, 102, 111, 204, 237, 303
SfaNI GCATC 3 cut(s) 138, 139, 255
SfuI TTCGAA 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 1 cut(s) 69
SsiI CCGC 3 cut(s) 152, 175, 262
SspMI CTAG 1 cut(s) 47
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 39, 237
TasI AATT 1 cut(s) 69
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 1 cut(s) 190
TseI GCWGC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 182, 307
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 190
XapI RAATTY 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.