MD14G1231900.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000159
Physical Location & Seq
Forward (+)
31227770 .. 31230877
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1231900.v1.1.491

Sequence Viewer

Length: 2733 bp
ATGGCGGAATTCGTGTCTACCATTGCCGAAAGTGTCATGGCAGGGCTGGCTTCCTTTGCTTACCAAGAAGTTTGCTTGGCATGGGGTGTCGAAGAGGAGCTAAAAAGGCTTCGGAATACCTTTTCCCACATCAAACTGTTGATCGTGGACGCTGAGGAAAGGCAGAGGAAGGAACCCCTTCTAACCCAATGGTTAGGAAGGCTCAAAGATGCTTGCTACGACATAGATGACGTGCTCGATGAGTTTGCATTCCAAGAAATGCAGATGAAAATGCTGGTCCCTGGCGGGAGTATCAAAGGAAAGGTATGCATATTCTTTTCGCGCTGGAATCCTATCATGTTTAACTTCATTATGGGGCATAAGATCAAAGAAATTAGAGAAAGGTTAGATGACATGGGAGATGAAAAATCTCGCTTTCATCTTGTGGAACGAGTTGGAGATTGGCAGGTTGTGCACATAAAGAGGGAGACTCACTCTTTTGTGCAGCCATATGATGTTATTGGAAGAGAGGATGATAAGAAACAGGTTATCATCCATCTCTTGAACTTGAACCTGAAGGAAGCAAGTGGCGGTGCCCCCGAGCATGTTTCTGTTATATCCATTATCGGATTAGGAGGCATAGGCAAGACCACTCTTGCTAAATTGGTGTATAATGATCATCGGGTTGTCAGAAACTTCGACATGAGAATGTGGGTGTGTGTTTCGGACCACTTTGATCACAAAAGATTGGTTCATGACATTCTTACTTCAGCAACTAACCAAAACTGTGGTGATCATGAAAGCTTAGACCAAATGCAGAAAAAGTTGCAAGACGCTCTGAGGGATAAGCAGTTTCTGTTGGTGTTGGACGATGTTTGGGACAAGGCTCATGTCGGAGTAACATCCGCAAAGTGGTTTGATATGAAAGCTCTATTAAATGTTGGAGCCGAGGGGAGTAAAGTCATCGTAACAACTCGCAGTGAATCAGTTGCTTCGTTGATGAGCTCCGCGAGAGTGCATCAACTAAAAGGTCTTCCTCTCAAGGTTTGCAAGTCTTTGCTTATAAGACGGGCATTTGGTCAAAAAGGTGAGGAGCAACGCTATCAACACTTGATGGAGATTGCGGAAAGTATTGCAGATAAATGCGGTGGGGTTCCTCTAGCGGTAACCACACTAGGGAGCCTACTGCATTCAAAAAGGGAGAAACAGGTTTGGTCCAAAGTCAGAGACAGTGACATATGGACATTGGACCAGGGAAACGAAGACATTCTACCTGCACTGAAGTTAAGCTACGATGCATTGCCGCCCTACTTGAAGCCTTGTTTCGCCATTTGTTCACTGTTCCCGAAAGACTATGTGTTCCAAAGTACAGATTTGGTTTCGCTATGGATGGCACAAGGCTTCCTGCAATCCTCTAATCCAACAAGATCTAAAGGAGACCAAGAGTTGGAAGAGATTGGCCTAGACTACATCAGGCAATTAGGTTCGAGATTCTTATTTGAAATAGAGGAAGATTCGATTGATTTCATAGTATTTAAAATGCATAATCTTGTCCATGATTTAGCTATTTCAGTGTCAAAACTAGAGTGCTCTTCCATAAATTTCCGTCCTAACCCCGATACTTGTAAAAGGGTTCGGCATGTGTCAATGTCAAGGGATGACTTACCCACCAAGAAAGGGGAACGAGTTCCTGAGTTCTTTCTTCAGTTGCAGAAAGTTTGGACCATTCTATTTCCAATTCCAGGACATGAAGGATTCTCCAACAAATCTGTGTTGAAATCTTGCATCCTGAAATTCAAGTACCTACGGGTGCTCGATCTTAGAGGTTCAACTTTTGAGGAGTTGCCGGGTTCTGTTGGCAACTTGAGTGGCCTGAGATATCTCAACTTGTCCAAAAATCCTAGCATGCAAAAGCTTCCGGGTTCCATTTGCAGGCTGCTAAACTTGCAGACCCTACTACTTACTAATTGTGAGAAACTCAAGAAGCTGCCGAGAGACATAGGGAACCTGATCAACCTCAGAACATTGGTGCTGACAACAAATCAGACGGTTATGCCAGAAGGAATTGAACGCCTCACCTCTCTTCGATTTTTACAGGTCGACAGCTGCAGTCATCTTGTATCTTTCGGCGAAATGATGCAGCGCCTCACAAACCTCCGAGTGCTGGTTATAAGCAACTGTCAAAGTCTAGAGTCATTGCCAGGTGACTTGGAAAACCTGACAGCGTTAAAGGCTTTAGCCATCTCCGACTGCGTAAATCTTGACTTGATGAGGTCGGGAGAAGGCATTCGCGATCTTCGATCGGTTTCAGTCTCGCATTTAGAGGCTCTTCCCCCTTGGCTTAAAGAGTCTACAGACACTCTACAGAGCTTAAGTATCAAGAACTGCGAGGGCCTCAAGGAACTGCCGGAGTGGCTGCAAACCTTCAAGTTGCTGGAACATCTAGTGATCGAATACTGCCCTCAGCTGTCGAGCTTGCCGGAGGGGATGCATCTCCTCGGTGCATTGAGAGAGCTGAAGGTCGACGGGTGCTCGAAGTTGAGTGAAAGATGCAAAGGAAAAGAAGGTGCAGATTGGTTGAAGATCGTTCATGTCAGCAAGGTTACAGTTGATGGAGAGATTGACAATTTTGCTAACAAGGGTTTGCCTTCTTCAACTTTAGTATGGCATGATTCCTCTCTGTTGGTGGTTCATCCTGGTTTGTTCTTAGACTATGTTGGCTTTCTTAATTTAGACCTTTTAGTTTGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

911

Amino Acids

102.96

Weight (kDa)

6.55

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 90 9.4e-21 Rx N-terminal domain
NB-ARC PF00931 195 - 352 1.2e-31 NB-ARC domain
WHD_DRP PF23559 440 - 515 1.7e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 587 - 811 1.3e-26 Leucine-rich repeat region
LRR_8 PF13855 594 - 650 3.7e-06 Leucine rich repeat
LRR_RPS2 PF23247 684 - 795 1e-05 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1043, 2150
Acc36I ACCTGC 2 cut(s) 436, 1261
AccB1I GGYRCC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 1426
AccI GTMKAC 4 cut(s) 17, 2079, 2330, 2502
AccII CGCG 3 cut(s) 322, 989, 2271
AciI CCGC 9 cut(s) 5, 285, 570, 885, 987, 1103, 1125, 1142, 1283
AcsI RAATTY 3 cut(s) 8, 1579, 1772
AcuI CTGAAG 5 cut(s) 575, 732, 1280, 1667, 2516
AfaI GTAC 2 cut(s) 1348, 1781
AfiI CCNNNNNNNGG 7 cut(s) 891, 1155, 1426, 1656, 1721, 1911, 2143
AflII CTTAAG 1 cut(s) 2350
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 5 cut(s) 280, 1230, 1720, 2179, 2674
Alw21I GWGCWC 6 cut(s) 237, 456, 986, 1571, 1794, 2513
Alw26I GTCTC 5 cut(s) 461, 1200, 1410, 1968, 2296
Alw44I GTGCAC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 835
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 3 cut(s) 1438, 1849, 2371
ApaLI GTGCAC 1 cut(s) 452
ApeKI GCWGC 6 cut(s) 484, 1915, 1966, 2085, 2119, 2395
ApoI RAATTY 3 cut(s) 8, 1579, 1772
Asp700I GAANNNNTTC 4 cut(s) 177, 1245, 1665, 2265
AspLEI GCGC 2 cut(s) 324, 2124
AspS9I GGNCC 6 cut(s) 277, 706, 1194, 1228, 1701, 2371
AsuC2I CCSGG 2 cut(s) 1827, 1899
AsuHPI GGTGA 4 cut(s) 782, 1079, 2047, 2195
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 5 cut(s) 277, 706, 1194, 1228, 1701
BaeGI GKGCMC 2 cut(s) 456, 577
BanI GGYRCC 1 cut(s) 572
BanII GRGCYC 1 cut(s) 986
BarI GAAGNNNNNNTAC 4 cut(s) 1233, 1253, 1265, 1285
BbsI GAAGAC 2 cut(s) 1004, 1248
Bbv12I GWGCWC 6 cut(s) 237, 456, 986, 1571, 1794, 2513
BbvCI CCTCAGC 2 cut(s) 153, 2442
BbvI GCAGC 6 cut(s) 496, 1902, 1953, 2072, 2131, 2382
BccI CCATC 5 cut(s) 543, 1087, 1363, 2228, 2585
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 5 cut(s) 282, 1232, 1722, 2181, 2676
BclI TGATCA 4 cut(s) 655, 715, 772, 1989
BcnI CCSGG 2 cut(s) 1827, 1899
BcoDI GTCTC 5 cut(s) 461, 1200, 1410, 1968, 2296
BfaI CTAG 7 cut(s) 1139, 1154, 1442, 1562, 1881, 2168, 2423
BfmI CTRYAG 3 cut(s) 2086, 2331, 2342
BfoI RGCGCY 1 cut(s) 2125
BfrI CTTAAG 1 cut(s) 2350
BfuAI ACCTGC 2 cut(s) 436, 1261
BglI GCCNNNNNGGC 1 cut(s) 2392
BglII AGATCT 1 cut(s) 1406
BisI GCNGC 7 cut(s) 485, 1283, 1916, 1967, 2086, 2120, 2396
BlsI GCNGC 7 cut(s) 486, 1284, 1917, 1968, 2087, 2121, 2397
Bme1390I CCNGG 7 cut(s) 282, 1232, 1722, 1827, 1899, 2181, 2676
Bme18I GGWCC 5 cut(s) 277, 706, 1194, 1228, 1701
BmeT110I CYCGRG 1 cut(s) 578
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 6 cut(s) 277, 706, 1194, 1228, 1701, 2371
BmiI GGNNCC 8 cut(s) 174, 279, 574, 925, 1134, 1160, 1903, 1985
BmrFI CCNGG 7 cut(s) 282, 1232, 1722, 1827, 1899, 2181, 2676
BmsI GCATC 8 cut(s) 199, 1006, 1264, 1773, 2106, 2457, 2479, 2519
BpiI GAAGAC 2 cut(s) 1004, 1248
BplI GAGNNNNNCTC 4 cut(s) 454, 486, 458, 490
Bpu10I CCTNAGC 2 cut(s) 153, 2442
BpuEI CTTGAG 4 cut(s) 1004, 1864, 1943, 2360
BpuMI CCSGG 2 cut(s) 1827, 1899
BsaI GGTCTC 1 cut(s) 1410
BsaJI CCNNGG 5 cut(s) 280, 927, 1231, 2315, 2476
Bsc4I CCNNNNNNNGG 7 cut(s) 891, 1155, 1426, 1656, 1721, 1911, 2143
Bse3DI GCAATG 3 cut(s) 21, 1277, 2174
BseBI CCWGG 5 cut(s) 282, 1232, 1722, 2181, 2676
BseDI CCNNGG 5 cut(s) 280, 927, 1231, 2315, 2476
BseGI GGATG 8 cut(s) 517, 531, 881, 1374, 1642, 1764, 2472, 2671
BseLI CCNNNNNNNGG 7 cut(s) 891, 1155, 1426, 1656, 1721, 1911, 2143
BseMI GCAATG 3 cut(s) 21, 1277, 2174
BseMII CTCAG 6 cut(s) 144, 809, 1662, 1844, 2011, 2456
BseRI GAGGAG 4 cut(s) 110, 1085, 1832, 2465
BseSI GKGCMC 2 cut(s) 456, 577
BseXI GCAGC 6 cut(s) 496, 1902, 1953, 2072, 2131, 2382
BsgI GTGCAG 3 cut(s) 503, 1239, 2568
Bsh1236I CGCG 3 cut(s) 322, 989, 2271
Bsh1285I CGRYCG 1 cut(s) 2282
BshFI GGCC 3 cut(s) 1440, 1851, 2373
BshNI GGYRCC 1 cut(s) 572
BsiEI CGRYCG 1 cut(s) 2282
BsiHKAI GWGCWC 6 cut(s) 237, 456, 986, 1571, 1794, 2513
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 4 cut(s) 1826, 1898, 2387, 2459
BslFI GGGAC 2 cut(s) 263, 872
BslI CCNNNNNNNGG 7 cut(s) 891, 1155, 1426, 1656, 1721, 1911, 2143
BsmAI GTCTC 5 cut(s) 461, 1200, 1410, 1968, 2296
BsmFI GGGAC 2 cut(s) 263, 872
BsmI GAATGC 3 cut(s) 248, 1168, 2265
BsnI GGCC 3 cut(s) 1440, 1851, 2373
Bso31I GGTCTC 1 cut(s) 1410
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 7 cut(s) 237, 456, 577, 986, 1571, 1794, 2513
Bsp68I TCGCGA 1 cut(s) 2271
BspACI CCGC 9 cut(s) 5, 285, 570, 885, 987, 1103, 1125, 1142, 1283
BspANI GGCC 3 cut(s) 1440, 1851, 2373
BspCNI CTCAG 6 cut(s) 145, 810, 1663, 1845, 2010, 2455
BspFNI CGCG 3 cut(s) 322, 989, 2271
BspHI TCATGA 2 cut(s) 733, 775
BspLI GGNNCC 8 cut(s) 174, 279, 574, 925, 1134, 1160, 1903, 1985
BspMAI CTGCAG 1 cut(s) 2090
BspMI ACCTGC 2 cut(s) 436, 1261
BspQI GCTCTTC 2 cut(s) 1576, 2313
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 2350
BspTNI GGTCTC 1 cut(s) 1410
BsrDI GCAATG 3 cut(s) 21, 1277, 2174
BssECI CCNNGG 5 cut(s) 280, 927, 1231, 2315, 2476
BssT1I CCWWGG 1 cut(s) 2315
Bst2UI CCWGG 5 cut(s) 282, 1232, 1722, 2181, 2676
Bst4CI ACNGT 7 cut(s) 138, 767, 1211, 1320, 2029, 2159, 2587
Bst6I CTCTTC 6 cut(s) 87, 499, 1425, 1576, 2067, 2313
BstAFI CTTAAG 1 cut(s) 2350
BstAPI GCANNNNNTGC 1 cut(s) 451
BstC8I GCNNGC 5 cut(s) 48, 214, 1886, 1913, 2456
BstDEI CTNAG 9 cut(s) 153, 784, 818, 1671, 1799, 1853, 1997, 2442, 2686
BstEII GGTNACC 1 cut(s) 1144
BstF5I GGATG 8 cut(s) 517, 531, 881, 1374, 1642, 1764, 2472, 2671
BstFNI CGCG 3 cut(s) 322, 989, 2271
BstH2I RGCGCY 1 cut(s) 2125
BstHHI GCGC 2 cut(s) 324, 2124
BstMAI GTCTC 5 cut(s) 461, 1200, 1410, 1968, 2296
BstMCI CGRYCG 1 cut(s) 2282
BstMWI GCNNNNNNNGC 7 cut(s) 47, 56, 106, 451, 1924, 2210, 2392
BstNI CCWGG 5 cut(s) 282, 1232, 1722, 2181, 2676
BstNSI RCATGY 3 cut(s) 587, 1622, 1888
BstPI GGTNACC 1 cut(s) 1144
BstSCI CCNGG 7 cut(s) 280, 1230, 1720, 1825, 1897, 2179, 2674
BstSFI CTRYAG 3 cut(s) 2086, 2331, 2342
BstSLI GKGCMC 2 cut(s) 456, 577
BstUI CGCG 3 cut(s) 322, 989, 2271
BstV1I GCAGC 6 cut(s) 496, 1902, 1953, 2072, 2131, 2382
BstV2I GAAGAC 2 cut(s) 1004, 1248
BstX2I RGATCY 1 cut(s) 1406
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 1 cut(s) 1406
BsuRI GGCC 3 cut(s) 1440, 1851, 2373
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 8 cut(s) 517, 531, 881, 1374, 1642, 1764, 2472, 2671
BtsI GCAGTG 1 cut(s) 964
BtsIMutI CAGTG 5 cut(s) 964, 1216, 1256, 1316, 1557
BtuMI TCGCGA 1 cut(s) 2271
BveI ACCTGC 2 cut(s) 436, 1261
Cac8I GCNNGC 5 cut(s) 48, 214, 1886, 1913, 2456
CaiI CAGNNNCTG 1 cut(s) 835
CciI TCATGA 2 cut(s) 733, 775
CfoI GCGC 2 cut(s) 324, 2124
Cfr13I GGNCC 6 cut(s) 277, 706, 1194, 1228, 1701, 2371
CseI GACGC 2 cut(s) 158, 821
Csp6I GTAC 2 cut(s) 1347, 1780
CspCI CAANNNNNGTGG 2 cut(s) 1828, 1863
CviQI GTAC 2 cut(s) 1347, 1780
DdeI CTNAG 9 cut(s) 153, 784, 818, 1671, 1799, 1853, 1997, 2442, 2686
DraI TTTAAA 1 cut(s) 1516
Eam1104I CTCTTC 6 cut(s) 87, 499, 1425, 1576, 2067, 2313
EarI CTCTTC 6 cut(s) 87, 499, 1425, 1576, 2067, 2313
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 984
Eco130I CCWWGG 1 cut(s) 2315
Eco24I GRGCYC 1 cut(s) 986
Eco31I GGTCTC 1 cut(s) 1410
Eco32I GATATC 1 cut(s) 1859
Eco47I GGWCC 5 cut(s) 277, 706, 1194, 1228, 1701
Eco53kI GAGCTC 1 cut(s) 984
Eco57I CTGAAG 5 cut(s) 575, 732, 1280, 1667, 2516
Eco88I CYCGRG 1 cut(s) 578
Eco91I GGTNACC 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 984
EcoO109I RGGNCCY 1 cut(s) 2371
EcoO65I GGTNACC 1 cut(s) 1144
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 5 cut(s) 280, 1230, 1720, 2179, 2674
EcoRV GATATC 1 cut(s) 1859
EcoT14I CCWWGG 1 cut(s) 2315
EcoT22I ATGCAT 4 cut(s) 311, 1279, 1524, 2472
EcoT38I GRGCYC 1 cut(s) 986
ErhI CCWWGG 1 cut(s) 2315
FaqI GGGAC 2 cut(s) 263, 872
FauI CCCGC 1 cut(s) 278
FauNDI CATATG 2 cut(s) 490, 1217
FbaI TGATCA 4 cut(s) 655, 715, 772, 1989
FblI GTMKAC 4 cut(s) 17, 2079, 2330, 2502
Fnu4HI GCNGC 7 cut(s) 485, 1283, 1916, 1967, 2086, 2120, 2396
FokI GGATG 8 cut(s) 518, 524, 868, 1381, 1649, 1751, 2479, 2658
FriOI GRGCYC 1 cut(s) 986
Fsp4HI GCNGC 7 cut(s) 485, 1283, 1916, 1967, 2086, 2120, 2396
FspBI CTAG 7 cut(s) 1139, 1154, 1442, 1562, 1881, 2168, 2423
GlaI GCGC 2 cut(s) 323, 2123
GluI GCNGC 7 cut(s) 485, 1283, 1916, 1967, 2086, 2120, 2396
HaeII RGCGCY 1 cut(s) 2125
HaeIII GGCC 3 cut(s) 1440, 1851, 2373
HapII CCGG 4 cut(s) 1826, 1898, 2387, 2459
HgaI GACGC 2 cut(s) 158, 821
HhaI GCGC 2 cut(s) 324, 2124
Hin6I GCGC 2 cut(s) 322, 2122
HinP1I GCGC 2 cut(s) 322, 2122
HincII GTYRAC 2 cut(s) 2080, 2503
HindII GTYRAC 2 cut(s) 2080, 2503
HindIII AAGCTT 2 cut(s) 781, 1892
HinfI GANTC 9 cut(s) 328, 469, 962, 1470, 1493, 1734, 2171, 2327, 2651
HpaII CCGG 4 cut(s) 1826, 1898, 2387, 2459
HphI GGTGA 4 cut(s) 782, 1079, 2047, 2195
Hpy166II GTNNAC 7 cut(s) 18, 148, 454, 1316, 2080, 2331, 2503
Hpy8I GTNNAC 7 cut(s) 18, 148, 454, 1316, 2080, 2331, 2503
Hpy99I CGWCG 1 cut(s) 2507
HpyCH4III ACNGT 7 cut(s) 138, 767, 1211, 1320, 2029, 2159, 2587
HpyCH4IV ACGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 56, 106, 451, 1924, 2210, 2392
HpyF3I CTNAG 9 cut(s) 153, 784, 818, 1671, 1799, 1853, 1997, 2442, 2686
HpySE526I ACGT 1 cut(s) 231
HspAI GCGC 2 cut(s) 322, 2122
Ksp22I TGATCA 4 cut(s) 655, 715, 772, 1989
LguI GCTCTTC 2 cut(s) 1576, 2313
LmnI GCTCC 5 cut(s) 97, 923, 989, 1072, 1158
Lsp1109I GCAGC 6 cut(s) 496, 1902, 1953, 2072, 2131, 2382
LweI GCATC 8 cut(s) 199, 1006, 1264, 1773, 2106, 2457, 2479, 2519
MaeI CTAG 7 cut(s) 1139, 1154, 1442, 1562, 1881, 2168, 2423
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 6 cut(s) 877, 946, 1144, 1211, 2183, 2581
MflI RGATCY 1 cut(s) 1406
MhlI GDGCHC 7 cut(s) 237, 456, 577, 986, 1571, 1794, 2513
MlyI GAGTC 3 cut(s) 463, 2180, 2336
MmeI TCCRAC 8 cut(s) 415, 825, 853, 901, 1407, 1424, 1764, 2250
Mph1103I ATGCAT 4 cut(s) 311, 1279, 1524, 2472
MroXI GAANNNNTTC 4 cut(s) 177, 1245, 1665, 2265
MseI TTAA 9 cut(s) 342, 914, 1265, 1515, 2207, 2322, 2351, 2706, 2731
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 2085, 2446
MspCI CTTAAG 1 cut(s) 2350
MspI CCGG 4 cut(s) 1826, 1898, 2387, 2459
MspR9I CCNGG 7 cut(s) 282, 1232, 1722, 1827, 1899, 2181, 2676
Mva1269I GAATGC 3 cut(s) 248, 1168, 2265
MvaI CCWGG 5 cut(s) 282, 1232, 1722, 2181, 2676
MvnI CGCG 3 cut(s) 322, 989, 2271
MwoI GCNNNNNNNGC 7 cut(s) 47, 56, 106, 451, 1924, 2210, 2392
NciI CCSGG 2 cut(s) 1827, 1899
NdeI CATATG 2 cut(s) 490, 1217
NlaIV GGNNCC 8 cut(s) 174, 279, 574, 925, 1134, 1160, 1903, 1985
NmeAIII GCCGAG 2 cut(s) 952, 1995
NmuCI GTSAC 2 cut(s) 1211, 2183
NruI TCGCGA 1 cut(s) 2271
NsiI ATGCAT 4 cut(s) 311, 1279, 1524, 2472
NspI RCATGY 3 cut(s) 587, 1622, 1888
PaeI GCATGC 1 cut(s) 1888
PagI TCATGA 2 cut(s) 733, 775
PciSI GCTCTTC 2 cut(s) 1576, 2313
PcsI WCGNNNNNNNCGW 1 cut(s) 2275
PctI GAATGC 3 cut(s) 248, 1168, 2265
PdmI GAANNNNTTC 4 cut(s) 177, 1245, 1665, 2265
PfeI GAWTC 6 cut(s) 328, 962, 1470, 1493, 1734, 2651
PflMI CCANNNNNTGG 1 cut(s) 1426
PfoI TCCNGGA 1 cut(s) 1720
PkrI GCNGC 7 cut(s) 486, 1284, 1917, 1968, 2087, 2121, 2397
Ple19I CGATCG 1 cut(s) 2282
PleI GAGTC 3 cut(s) 463, 2179, 2335
PpsI GAGTC 3 cut(s) 463, 2179, 2335
PsiI TTATAA 2 cut(s) 1043, 2150
Psp124BI GAGCTC 1 cut(s) 986
Psp6I CCWGG 5 cut(s) 280, 1230, 1720, 2179, 2674
PspEI GGTNACC 1 cut(s) 1144
PspGI CCWGG 5 cut(s) 280, 1230, 1720, 2179, 2674
PspN4I GGNNCC 8 cut(s) 174, 279, 574, 925, 1134, 1160, 1903, 1985
PspPI GGNCC 6 cut(s) 277, 706, 1194, 1228, 1701, 2371
PstI CTGCAG 1 cut(s) 2090
PstNI CAGNNNCTG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 1406
PvuI CGATCG 1 cut(s) 2282
PvuII CAGCTG 2 cut(s) 2085, 2446
RruI TCGCGA 1 cut(s) 2271
RsaI GTAC 2 cut(s) 1348, 1781
RsaNI GTAC 2 cut(s) 1347, 1780
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 986
SalI GTCGAC 2 cut(s) 2078, 2501
SapI GCTCTTC 2 cut(s) 1576, 2313
SaqAI TTAA 9 cut(s) 342, 914, 1265, 1515, 2207, 2322, 2351, 2706, 2731
SatI GCNGC 7 cut(s) 485, 1283, 1916, 1967, 2086, 2120, 2396
Sau96I GGNCC 6 cut(s) 277, 706, 1194, 1228, 1701, 2371
SchI GAGTC 3 cut(s) 463, 2180, 2336
ScrFI CCNGG 7 cut(s) 282, 1232, 1722, 1827, 1899, 2181, 2676
SduI GDGCHC 7 cut(s) 237, 456, 577, 986, 1571, 1794, 2513
SfaNI GCATC 8 cut(s) 199, 1006, 1264, 1773, 2106, 2457, 2479, 2519
SfcI CTRYAG 3 cut(s) 2086, 2331, 2342
SinI GGWCC 5 cut(s) 277, 706, 1194, 1228, 1701
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 5 cut(s) 1019, 1843, 1958, 2350, 2375
SmoI CTYRAG 5 cut(s) 1019, 1843, 1958, 2350, 2375
SphI GCATGC 1 cut(s) 1888
SsiI CCGC 9 cut(s) 5, 285, 570, 885, 987, 1103, 1125, 1142, 1283
SspMI CTAG 7 cut(s) 1139, 1154, 1442, 1562, 1881, 2168, 2423
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 7 cut(s) 280, 1230, 1720, 1825, 1897, 2179, 2674
StyI CCWWGG 1 cut(s) 2315
TaaI ACNGT 7 cut(s) 138, 767, 1211, 1320, 2029, 2159, 2587
TaiI ACGT 1 cut(s) 234
TatI WGTACW 1 cut(s) 1346
TauI GCSGC 1 cut(s) 1285
TfiI GAWTC 6 cut(s) 328, 962, 1470, 1493, 1734, 2651
Tru1I TTAA 9 cut(s) 342, 914, 1265, 1515, 2207, 2322, 2351, 2706, 2731
Tru9I TTAA 9 cut(s) 342, 914, 1265, 1515, 2207, 2322, 2351, 2706, 2731
TscAI CASTG 5 cut(s) 964, 1216, 1263, 1323, 1557
TseFI GTSAC 2 cut(s) 1211, 2183
TseI GCWGC 6 cut(s) 484, 1915, 1966, 2085, 2119, 2395
Tsp45I GTSAC 2 cut(s) 1211, 2183
TspGWI ACGGA 1 cut(s) 1574
TspRI CASTG 5 cut(s) 964, 1216, 1263, 1323, 1557
Van91I CCANNNNNTGG 1 cut(s) 1426
Vha464I CTTAAG 1 cut(s) 2350
VneI GTGCAC 1 cut(s) 452
VpaK11BI GGWCC 5 cut(s) 277, 706, 1194, 1228, 1701
XapI RAATTY 3 cut(s) 8, 1579, 1772
XbaI TCTAGA 1 cut(s) 2167
XceI RCATGY 3 cut(s) 587, 1622, 1888
XmiI GTMKAC 4 cut(s) 17, 2079, 2330, 2502
XmnI GAANNNNTTC 4 cut(s) 177, 1245, 1665, 2265
XspI CTAG 7 cut(s) 1139, 1154, 1442, 1562, 1881, 2168, 2423
Zsp2I ATGCAT 4 cut(s) 311, 1279, 1524, 2472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.