Rorug03G0352400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000159
Physical Location & Seq
Forward (+)
43176068 .. 43177881
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0352400.1

Sequence Viewer

Length: 579 bp
ATGGCGGAAGAGAAGCCTGAAGTGCTTGACGCGGTGCTGAAGGAAACAGTTGATTTGGAAAACATACCCATTGAGGAGGTGCTTGAGAATCTGAGATGTAGCAAAGAGGGTCTCAGCAGTGAGGCTGCTGAGGAAAGATTAGCCATTTTTGGGCATAACAAGCTTGAGGAAAAGCAGTCTTTTGTTGATGGTTGCGCTGATGGAATGATGTTTGGAGCACTTAGTACTTGCCCACTCTGTTCCAGTCATCTTCACTATTCTGGAGCCATGTACAGGTGCCATGGGTTCCTGACTGCATGGAGCAAGTGTTCTTACTCTACTCATGAACCTGAACGTCGTAAAGGGAAGTGGAAAATCCCAGAAGATACAGATAATCAATTTCTGCAAAAGTTAGCAGAGTTGATGAAGATGCTCTTCAACGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAGATGCCACTTGGAAAACTGAGTAAAAGCAATATCCAAAAGGCTTTCTTCCGGTATTCTATTTTCTCTATTCAGGGTTTGAACGGAAATACAGAATCTCTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

192

Amino Acids

21.85

Weight (kDa)

5.26

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 21 - 58 4.6e-09 Cation transporter/ATPase, N-terminus
Zn_ribbon_PADR1 PF08063 73 - 113 6.6e-15 PADR1 domain, zinc ribbon fold
PARP_reg PF02877 129 - 172 2.1e-12 Poly(ADP-ribose) polymerase, regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 276
AccII CGCG 1 cut(s) 32
AciI CCGC 2 cut(s) 5, 32
AclI AACGTT 1 cut(s) 420
AcsI RAATTY 1 cut(s) 449
AcuI CTGAAG 2 cut(s) 39, 59
AfaI GTAC 2 cut(s) 226, 272
AfiI CCNNNNNNNGG 2 cut(s) 150, 273
AgsI TTSAA 3 cut(s) 418, 425, 550
AloI GAACNNNNNNTCC 2 cut(s) 292, 324
AluBI AGCT 2 cut(s) 163, 437
AluI AGCT 2 cut(s) 163, 437
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 125, 437
ApoI RAATTY 1 cut(s) 449
AspLEI GCGC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 220
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 112, 424
BccI CCATC 3 cut(s) 182, 194, 439
BcoDI GTCTC 1 cut(s) 116
BisI GCNGC 2 cut(s) 126, 438
BlsI GCNGC 2 cut(s) 127, 439
BmcAI AGTACT 1 cut(s) 226
BmiI GGNNCC 3 cut(s) 265, 278, 287
BmsI GCATC 2 cut(s) 399, 462
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 104, 185
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 273
Bse1I ACTGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 280
BseLI CCNNNNNNNGG 2 cut(s) 150, 273
BseMII CTCAG 4 cut(s) 83, 120, 127, 479
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 2 cut(s) 112, 424
Bsh1236I CGCG 1 cut(s) 32
BshNI GGYRCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 1 cut(s) 520
BslI CCNNNNNNNGG 2 cut(s) 150, 273
BsmAI GTCTC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 220
Bsp1407I TGTACA 1 cut(s) 270
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 2 cut(s) 5, 32
BspCNI CTCAG 4 cut(s) 84, 121, 126, 480
BspFNI CGCG 1 cut(s) 32
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 265, 278, 287
BspQI GCTCTTC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 276
BspTNI GGTCTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 270
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 280
BssT1I CCWWGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 2 cut(s) 3, 419
BstAUI TGTACA 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 92, 113, 129, 221, 488
BstDSI CCRYGG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 197
BstMAI GTCTC 1 cut(s) 116
BstMWI GCNNNNNNNGC 2 cut(s) 22, 160
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 112, 424
BtgI CCRYGG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 124
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 197
CseI GACGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 225, 271
CviAII CATG 5 cut(s) 268, 281, 297, 323, 442
CviJI RGCY 7 cut(s) 16, 125, 143, 163, 266, 437, 512
CviKI_1 RGCY 7 cut(s) 16, 125, 143, 163, 266, 437, 512
CviQI GTAC 2 cut(s) 225, 271
DdeI CTNAG 5 cut(s) 92, 113, 129, 221, 488
Eam1104I CTCTTC 2 cut(s) 3, 419
EarI CTCTTC 2 cut(s) 3, 419
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 39, 59
EcoT14I CCWWGG 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 5 cut(s) 271, 284, 300, 326, 445
FaiI YATR 9 cut(s) 65, 156, 269, 282, 298, 324, 430, 443, 464
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 500, 532
FatI CATG 5 cut(s) 267, 280, 296, 322, 441
Fnu4HI GCNGC 2 cut(s) 126, 438
Fsp4HI GCNGC 2 cut(s) 126, 438
GlaI GCGC 1 cut(s) 196
GluI GCNGC 2 cut(s) 126, 438
GsuI CTGGAG 1 cut(s) 282
HapII CCGG 1 cut(s) 520
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 197
Hin1II CATG 5 cut(s) 271, 284, 300, 326, 445
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 2 cut(s) 88, 563
HpaII CCGG 1 cut(s) 520
Hpy188I TCNGA 2 cut(s) 93, 469
Hpy188III TCNNGA 3 cut(s) 261, 289, 323
Hpy99I CGWCG 1 cut(s) 339
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 2 cut(s) 334, 420
HpyCH4V TGCA 2 cut(s) 296, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 160
HpyF3I CTNAG 5 cut(s) 92, 113, 129, 221, 488
HpySE526I ACGT 2 cut(s) 334, 420
Hsp92II CATG 5 cut(s) 271, 284, 300, 326, 445
HspAI GCGC 1 cut(s) 195
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 3 cut(s) 215, 263, 300
LpnPI CCDG 9 cut(s) 30, 246, 256, 259, 302, 342, 372, 527, 533
Lsp1109I GCAGC 2 cut(s) 112, 424
LweI GCATC 2 cut(s) 399, 462
MaeII ACGT 2 cut(s) 334, 420
MboII GAAGA 6 cut(s) 20, 242, 374, 406, 418, 508
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 2 cut(s) 377, 449
MnlI CCTC 6 cut(s) 67, 70, 100, 115, 124, 160
MseI TTAA 1 cut(s) 570
MspI CCGG 1 cut(s) 520
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 22, 160
NcoI CCATGG 1 cut(s) 280
NlaIII CATG 5 cut(s) 271, 284, 300, 326, 445
NlaIV GGNNCC 3 cut(s) 265, 278, 287
PagI TCATGA 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 88, 563
PkrI GCNGC 2 cut(s) 127, 439
Psp1406I AACGTT 1 cut(s) 420
PspN4I GGNNCC 3 cut(s) 265, 278, 287
RsaI GTAC 2 cut(s) 226, 272
RsaNI GTAC 2 cut(s) 225, 271
SapI GCTCTTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 2 cut(s) 126, 438
ScaI AGTACT 1 cut(s) 226
SduI GDGCHC 1 cut(s) 220
SetI ASST 7 cut(s) 81, 165, 278, 331, 337, 423, 439
SfaNI GCATC 2 cut(s) 399, 462
SmlI CTYRAG 2 cut(s) 83, 164
SmoI CTYRAG 2 cut(s) 83, 164
Sse9I AATT 2 cut(s) 377, 449
SsiI CCGC 2 cut(s) 5, 32
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 2 cut(s) 337, 423
TasI AATT 2 cut(s) 377, 449
TatI WGTACW 2 cut(s) 224, 270
TfiI GAWTC 2 cut(s) 88, 563
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 124
TseI GCWGC 2 cut(s) 125, 437
TspDTI ATGAA 2 cut(s) 339, 419
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 1 cut(s) 124
XapI RAATTY 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.