Rh2AG263400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000159
Physical Location & Seq
Forward (+)
30413753 .. 30414742
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG263400.1

Sequence Viewer

Length: 990 bp
ATGCTCAAGTCTAAGAAACTGCGAACCATTCTAATTCCTGAAGAAGGTCAGATGAGTCAACGTTTTGTAAAGACATGCATTTCGAGATTCAAATATATGCGGGTGCTAGATCTTGGGGGGTCGCATCTTGAGGAGCTACCAAGTTCCATCGTTAGTTTGTTTCATTTGAGATATCTCGACTTGTCTAATAATAGGAAGATAAAAAGGCTTCCCAATTTCATCAGTAAGCTGCTGAATTTGGAGACCTTGAACTCTGGGGGTTGTGACGCACTTGAGGAGATACCCAAAGACATAGGGAACCTGATCAAGCTCCGAAGCTTGCTGATAACTACACAACAGACGTATTTGCCAAAAGGAATTAGACGCCTCACCTCACTTCAATATTTATGGTTTGATGGATGTGTCAATCTTAAATCTTTGGGCGAAGAGATCCAATTCCTCAACAACCTTCGCACTTTGTCGATTAGAAGGTGTAATAATTTGGCATCCTTGCCACCAAATATGAAACACATGACCGCTTTAGATACTTTGGGTGTCCATGATTGCCCGATGCTTCAGTTGATGATGAGATCAGGGGAAGGTCCTCAACGTCTTCGATCATTGCTTATTATGGATTCAAGTTTGGAGGCGTTGCCCCCTTGGCTCATTGAAGATTCTGCAGACACTCTACAGAGCATAGCACTTGAAGGATGTCATAATCTCACGGCACTTCCGGAGTTGATAAAGTTCACATTCCTCGAGCAACTACGCATTGAAGACTGCTCCAAATTGTCGGCTTTGCCGCAAGAGTTGCATTGCCTTACTGAGTTGAGAGAATTGAAGATTATTGGTTGTCCTGAATTGAGCAAAAGCTGCAAAAGGCAATTAAAAGGTGAGGAGTGGTCAAAGATGGCACGTCAGGCAAAGATTACACTCGACGACTCTGATGATGAAGCCGACAATCGCTTGGCCGACGGTAGGATCGCGTACCACACGCGACGACGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

329

Amino Acids

37.64

Weight (kDa)

8.87

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 26 - 227 6.5e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 195 - 254 1.9e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 965, 976
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 3 cut(s) 100, 516, 782
AclI AACGTT 1 cut(s) 61
AclWI GGATC 2 cut(s) 424, 968
AcoI YGGCCR 1 cut(s) 948
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 60, 539
AcyI GRCGYC 2 cut(s) 364, 982
AfaI GTAC 1 cut(s) 968
AfiI CCNNNNNNNGG 2 cut(s) 44, 957
AgsI TTSAA 8 cut(s) 91, 250, 380, 618, 650, 686, 755, 820
AjiI CACGTC 1 cut(s) 896
AluBI AGCT 5 cut(s) 136, 229, 310, 318, 852
AluI AGCT 5 cut(s) 136, 229, 310, 318, 852
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 424, 968
Ama87I CYCGRG 1 cut(s) 737
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 1 cut(s) 948
ApeKI GCWGC 2 cut(s) 229, 852
ApoI RAATTY 1 cut(s) 235
ArsI GACNNNNNNTTYG 2 cut(s) 64, 96
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 361, 884
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 581
BbsI GAAGAC 2 cut(s) 584, 762
BbvI GCAGC 2 cut(s) 216, 839
BccI CCATC 3 cut(s) 155, 389, 883
BceAI ACGGC 2 cut(s) 720, 969
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 2 cut(s) 657, 668
BglI GCCNNNNNGGC 1 cut(s) 640
BglII AGATCT 1 cut(s) 109
BisI GCNGC 3 cut(s) 230, 782, 853
BlsI GCNGC 3 cut(s) 231, 783, 854
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 1 cut(s) 737
BmgBI CACGTC 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 299
BmsI GCATC 3 cut(s) 133, 494, 540
BpiI GAAGAC 2 cut(s) 584, 762
BpuEI CTTGAG 2 cut(s) 149, 293
BsaHI GRCGYC 2 cut(s) 364, 982
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 638
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 957
Bse3DI GCAATG 2 cut(s) 599, 793
BseAI TCCGGA 1 cut(s) 712
BseDI CCNNGG 1 cut(s) 638
BseGI GGATG 3 cut(s) 404, 485, 695
BseLI CCNNNNNNNGG 2 cut(s) 44, 957
BseMI GCAATG 2 cut(s) 599, 793
BseMII CTCAG 1 cut(s) 795
BseRI GAGGAG 3 cut(s) 146, 290, 890
BseXI GCAGC 2 cut(s) 216, 839
Bsh1236I CGCG 2 cut(s) 965, 976
BshFI GGCC 1 cut(s) 950
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 713
BslI CCNNNNNNNGG 2 cut(s) 44, 957
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 1 cut(s) 950
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 737
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 6 cut(s) 109, 303, 429, 569, 596, 960
BspACI CCGC 3 cut(s) 100, 516, 782
BspANI GGCC 1 cut(s) 950
BspCNI CTCAG 1 cut(s) 796
BspEI TCCGGA 1 cut(s) 712
BspFNI CGCG 2 cut(s) 965, 976
BspLI GGNNCC 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 661
BspPI GGATC 2 cut(s) 424, 968
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 599, 793
BssECI CCNNGG 1 cut(s) 638
BssMI GATC 6 cut(s) 109, 303, 429, 569, 596, 960
BssNI GRCGYC 2 cut(s) 364, 982
BssT1I CCWWGG 1 cut(s) 638
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 2 cut(s) 364, 982
BstAPI GCANNNNNTGC 2 cut(s) 790, 852
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 12, 804
BstENI CCTNNNNNAGG 1 cut(s) 42
BstF5I GGATG 3 cut(s) 404, 485, 695
BstFNI CGCG 2 cut(s) 965, 976
BstKTI GATC 6 cut(s) 112, 306, 432, 572, 599, 963
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 6 cut(s) 109, 303, 429, 569, 596, 960
BstMWI GCNNNNNNNGC 4 cut(s) 640, 790, 852, 899
BstNSI RCATGY 1 cut(s) 78
BstSFI CTRYAG 2 cut(s) 657, 668
BstUI CGCG 2 cut(s) 965, 976
BstV1I GCAGC 2 cut(s) 216, 839
BstV2I GAAGAC 2 cut(s) 584, 762
BstX2I RGATCY 2 cut(s) 109, 429
BstYI RGATCY 2 cut(s) 109, 429
BsuRI GGCC 1 cut(s) 950
BtrI CACGTC 1 cut(s) 896
BtsCI GGATG 3 cut(s) 404, 485, 695
Cac8I GCNNGC 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 2 cut(s) 275, 372
Csp6I GTAC 1 cut(s) 967
CviAII CATG 3 cut(s) 75, 511, 539
CviQI GTAC 1 cut(s) 967
DdeI CTNAG 2 cut(s) 12, 804
DpnI GATC 6 cut(s) 111, 305, 431, 571, 598, 962
DpnII GATC 6 cut(s) 109, 303, 429, 569, 596, 960
EaeI YGGCCR 1 cut(s) 948
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco130I CCWWGG 1 cut(s) 638
Eco31I GGTCTC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 581
Eco57I CTGAAG 2 cut(s) 60, 539
Eco88I CYCGRG 1 cut(s) 737
EcoNI CCTNNNNNAGG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 581
EcoRV GATATC 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 638
EcoT22I ATGCAT 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 638
FaeI CATG 3 cut(s) 78, 514, 542
FatI CATG 3 cut(s) 74, 510, 538
FauI CCCGC 1 cut(s) 93
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 230, 782, 853
FokI GGATG 3 cut(s) 411, 472, 702
Fsp4HI GCNGC 3 cut(s) 230, 782, 853
FspBI CTAG 1 cut(s) 107
GluI GCNGC 3 cut(s) 230, 782, 853
HaeIII GGCC 1 cut(s) 950
HapII CCGG 1 cut(s) 713
HgaI GACGC 2 cut(s) 275, 372
Hin1I GRCGYC 2 cut(s) 364, 982
Hin1II CATG 3 cut(s) 78, 514, 542
HincII GTYRAC 1 cut(s) 59
HindII GTYRAC 1 cut(s) 59
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 5 cut(s) 55, 87, 614, 653, 920
HpaII CCGG 1 cut(s) 713
HphI GGTGA 2 cut(s) 361, 884
Hpy166II GTNNAC 2 cut(s) 59, 729
Hpy188I TCNGA 3 cut(s) 51, 314, 925
Hpy188III TCNNGA 6 cut(s) 38, 84, 128, 176, 713, 836
Hpy8I GTNNAC 2 cut(s) 59, 729
Hpy99I CGWCG 4 cut(s) 920, 956, 981, 984
HpyAV CCTTC 5 cut(s) 38, 458, 462, 572, 680
HpyCH4III ACNGT 1 cut(s) 956
HpyCH4IV ACGT 4 cut(s) 61, 341, 589, 895
HpyCH4V TGCA 4 cut(s) 78, 659, 793, 855
HpyF10VI GCNNNNNNNGC 4 cut(s) 640, 790, 852, 899
HpyF3I CTNAG 2 cut(s) 12, 804
HpySE526I ACGT 4 cut(s) 61, 341, 589, 895
Hsp92I GRCGYC 2 cut(s) 364, 982
Hsp92II CATG 3 cut(s) 78, 514, 542
Kpn2I TCCGGA 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 6 cut(s) 109, 303, 429, 569, 596, 960
LmnI GCTCC 3 cut(s) 133, 315, 767
LpnPI CCDG 7 cut(s) 51, 240, 314, 558, 726, 849, 884
Lsp1109I GCAGC 2 cut(s) 216, 839
LweI GCATC 3 cut(s) 133, 494, 540
MaeI CTAG 1 cut(s) 107
MaeII ACGT 4 cut(s) 61, 341, 589, 895
MaeIII GTNAC 1 cut(s) 263
MalI GATC 6 cut(s) 111, 305, 431, 571, 598, 962
MboI GATC 6 cut(s) 109, 303, 429, 569, 596, 960
MboII GAAGA 7 cut(s) 53, 208, 437, 584, 662, 767, 832
MflI RGATCY 2 cut(s) 109, 429
MlyI GAGTC 2 cut(s) 64, 914
MnlI CCTC 9 cut(s) 124, 268, 377, 382, 449, 594, 619, 746, 868
Mph1103I ATGCAT 1 cut(s) 80
MroI TCCGGA 1 cut(s) 712
MseI TTAA 2 cut(s) 411, 866
MspI CCGG 1 cut(s) 713
MvnI CGCG 2 cut(s) 965, 976
MwoI GCNNNNNNNGC 4 cut(s) 640, 790, 852, 899
NdeII GATC 6 cut(s) 109, 303, 429, 569, 596, 960
NlaIII CATG 3 cut(s) 78, 514, 542
NlaIV GGNNCC 1 cut(s) 299
NmuCI GTSAC 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 80
NspI RCATGY 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 737
PfeI GAWTC 3 cut(s) 87, 614, 653
PkrI GCNGC 3 cut(s) 231, 783, 854
PleI GAGTC 2 cut(s) 63, 914
PpsI GAGTC 2 cut(s) 63, 914
PpuMI RGGWCCY 1 cut(s) 581
Psp1406I AACGTT 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PspXI VCTCGAGB 1 cut(s) 737
PstI CTGCAG 1 cut(s) 661
PsuI RGATCY 2 cut(s) 109, 429
RsaI GTAC 1 cut(s) 968
RsaNI GTAC 1 cut(s) 967
SaqAI TTAA 2 cut(s) 411, 866
SatI GCNGC 3 cut(s) 230, 782, 853
Sau3AI GATC 6 cut(s) 109, 303, 429, 569, 596, 960
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 2 cut(s) 64, 914
SfaNI GCATC 3 cut(s) 133, 494, 540
SfcI CTRYAG 2 cut(s) 657, 668
Sfr274I CTCGAG 1 cut(s) 737
SinI GGWCC 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 737
SmlI CTYRAG 4 cut(s) 5, 128, 272, 737
SmoI CTYRAG 4 cut(s) 5, 128, 272, 737
SsiI CCGC 3 cut(s) 100, 516, 782
SspI AATATT 1 cut(s) 383
SspMI CTAG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 638
TaaI ACNGT 1 cut(s) 956
TaiI ACGT 4 cut(s) 64, 344, 592, 898
TaqI TCGA 6 cut(s) 83, 177, 461, 595, 738, 915
TauI GCSGC 1 cut(s) 784
TfiI GAWTC 3 cut(s) 87, 614, 653
Tru1I TTAA 2 cut(s) 411, 866
Tru9I TTAA 2 cut(s) 411, 866
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 2 cut(s) 229, 852
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 4 cut(s) 152, 208, 518, 945
VpaK11BI GGWCC 1 cut(s) 581
XagI CCTNNNNNAGG 1 cut(s) 42
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 737
XspI CTAG 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.