Rroxscaffold_2G00127350

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000159
Physical Location & Seq
Forward (+)
62815454 .. 62816338
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127350.1

Sequence Viewer

Length: 885 bp
ATGGAGTTCGTCTACAGCATTGCAGACAACGTCTTGGATAGGCTAGCTTCACATGCTTCCCAAGAGATCTCCTTGGCATGGGGTGCCCAACTTGAACTCACCAAGCTCAACAAGACCTTATCTACCATCAAACTAGTGCTTGAAGATGCGGAGAAGAAGCAAGTGAGGAATTCCCTAATCACTCGTTGGTTGGGAAATCTCAAAGATGTTTGTCATGACATTGATGATGTCTTAGACGAACTGGAGTTCCAAAAGTTGCGGTTGAAAGTGGAGGTCGACAACTGCAGGAAGATCAAAAGAAAGGTACGCCAATTTTTTTCCCGATGGAATCCAGTGGTGTTTAATTTCAAAATGGGACATAAAGTGAAACAGATTAGAGAAAGACTAGTTGAAATTGATAATGAAAAGAGTGAGTTTGCTCTCTTTGAGTTTGCTAAGATAGCTGAAGATCCTAGTGTGCTCCGAGGGATGCACGATAATAAAAGAGAGACGGACTCCTTGGTGGAGGCTTCAGATGTAATTGGCAGAGATGATGATAAAAAACAGATTATTAGTCATCTCTTAAATAACACTGATATCTCTAATGAGGAGAATGTTTCTGTTATTTCCATCCTCGGGTTAGGAGGGTTGGGAAAGACAACTCTCGCTAAATTAGTGTATAATGATAGCATGGTGGAGGAGAATTTCGACATGAAAATGTGGATATGCGTCTCAAACAACTTTGATATCCAAACATTAATTTGCTGCATTATCAATGCTGTAATTGAACAAAAATATGAGGATAAAAGTTTGGACCTTATGAAAAGAAGATTGCAAGATACTTTGCGAGGTAGAAGATTTTTGTTAGTGTTGGATGATGTCTGGGATATGGAGCGATTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

294

Amino Acids

34.19

Weight (kDa)

5.47

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 96 3.2e-20 Rx N-terminal domain
NB-ARC PF00931 178 - 293 1e-25 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000159)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22800 FvH4_1g23030 FvH4_2g17621 FvH4_2g17623 FvH4_2g17640 FvH4_3g16170 FvH4_4g15173 FvH4_4g15190 FvH4_5g16900 FvH4_6g51550 FvH4_6g51570 FvH4_6g51610 FvH4_6g51610 FvH4_7g31270 FvH4_7g31321 FvH4_7g31321
malus_domestica MD06G1096400.v1.1 MD14G1231900.v1.1
prunus_persica Prupe.5G111200_v2.0.a1 Prupe.5G227800_v2.0.a1
pyrus_communis pycom06g09270
rosa_chinensis RchiOBHm_Chr2g0116281 RchiOBHm_Chr2g0116321 RchiOBHm_Chr2g0116651 RchiOBHm_Chr2g0116681 RchiOBHm_Chr2g0116691 RchiOBHm_Chr2g0116761 RchiOBHm_Chr2g0116981 RchiOBHm_Chr2g0117271 RchiOBHm_Chr2g0117561 RchiOBHm_Chr2g0117671 RchiOBHm_Chr5g0009011 RchiOBHm_Chr5g0009021 RchiOBHm_Chr5g0009031 RchiOBHm_Chr5g0051501 RchiOBHm_Chr5g0059261 RchiOBHm_Chr7g0179121 RchiOBHm_Chr7g0179151 RchiOBHm_Chr7g0180011 RchiOBHm_Chr7g0180031
rosa_laevigata RLG00000018253 RLG00000018256 RLG00000018298 RLG00000018318 RLG00000018339 RLG00000018341 RLG00000018355 RLG00000018360 RLG00000018363 RLG00000018364 RLG00000021097
rosa_multiflora Rmu_co8108666.1_g000001 Rmu_co8113786.1_g000001 Rmu_co8303371.1_g000001 Rmu_co8353409.1_g000001 Rmu_sc0000888.1_g000020 Rmu_sc0001035.1_g000046 Rmu_sc0001617.1_g000028 Rmu_sc0001617.1_g000031 Rmu_sc0002009.1_g000005 Rmu_sc0002009.1_g000024 Rmu_sc0002034.1_g000013 Rmu_sc0002191.1_g000001 Rmu_sc0002983.1_g000027 Rmu_sc0003499.1_g000025 Rmu_sc0004602.1_g000012 Rmu_sc0004795.1_g000010 Rmu_sc0004795.1_g000017 Rmu_sc0004888.1_g000050 Rmu_sc0004888.1_g000052 Rmu_sc0004888.1_g000054 Rmu_sc0004942.1_g000014 Rmu_sc0004942.1_g000015 Rmu_sc0004942.1_g000017 Rmu_sc0004942.1_g000020 Rmu_sc0005792.1_g000003 Rmu_sc0006184.1_g000014 Rmu_sc0006730.1_g000011 Rmu_sc0006730.1_g000012 Rmu_sc0011574.1_g000001 Rmu_sc0011574.1_g000002 Rmu_sc0011969.1_g000003 Rmu_sc0016190.1_g000001 Rmu_sc0016190.1_g000002 Rmu_sc0020362.1_g000003 Rmu_sc0020362.1_g000004 Rmu_sc0020362.1_g000005 Rmu_sc0025557.1_g000002 Rmu_sc0031855.1_g000001 Rmu_ssc0000119.1_g000002 Rmu_ssc0000119.1_g000006 Rmu_ssc0000119.1_g000008 Rmu_ssc0000119.1_g000015 Rmu_ssc0000119.1_g000018 Rmu_ssc0000119.1_g000023 Rmu_ssc0000238.1_g000010 Rmu_ssc0000238.1_g000014
rosa_roxburghii Rroxscaffold_1G00067000 Rroxscaffold_1G00067640 Rroxscaffold_2G00126270 Rroxscaffold_2G00126830 Rroxscaffold_2G00126970 Rroxscaffold_2G00127350 Rroxscaffold_3G00273210 Rroxscaffold_3G00273240 Rroxscaffold_3G00274260 Rroxscaffold_3G00274370 Rroxscaffold_4G00321190
rosa_rugosa Rorug02G0205600 Rorug02G0209300 Rorug02G0209500 Rorug02G0211700 Rorug02G0212300 Rorug02G0213200 Rorug02G0213200 Rorug02G0213400 Rorug03G0352400 Rorug03G0352500 Rorug04G0440900 Rorug05G0171300 Rorug05G0322100 Rorug06G0421200 Rorug06G0421400 Rorug06G0421500 Rorug06G0421600 Rorug06G0429800 Rorug06G0430000 Rorug06G0430100
rosa_samantha Rh1DG100600 Rh2AG260900 Rh2AG261000 Rh2AG263400 Rh2AG263600 Rh2AG263900 Rh2AG265300 Rh2AG269300 Rh2AG269700 Rh2BG281000 Rh2CG267300 Rh2CG267500 Rh2CG270600 Rh2CG270700 Rh2CG271100 Rh2DG274100 Rh2DG290500 Rh2DG291600 Rh4DG089700 Rh5CG077900 Rh5CG421700 Rh5DG065900 Rh5DG066000 Rh7BG020200 Rh7BG020700 Rh7BG029700 Rh7BG029800 Rh7BG029900 Rh7BG030100 Rh7CG031400 Rh7CG031900 Rh7DG021300 Rh7DG030600 Rh7DG030900
rosa_wichuraiana Rw0G008860 Rw1G007590 Rw2G020460 Rw2G020740 Rw2G020770 Rw2G020820 Rw2G020920 Rw2G021100 Rw2G021120 Rw2G021260 Rw2G021280 Rw2G021290 Rw5G006520 Rw5G028690 Rw5G036360 Rw5G036370 Rw7G001730 Rw7G002430 Rw7G002450 Rw7G002470 Rw7G002510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 12, 276
AciI CCGC 2 cut(s) 149, 259
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 2 cut(s) 169, 682
AcuI CTGAAG 2 cut(s) 465, 495
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 78, 615
AgsI TTSAA 6 cut(s) 95, 143, 265, 349, 392, 767
AhlI ACTAGT 2 cut(s) 133, 385
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
AluBI AGCT 3 cut(s) 47, 106, 443
AluI AGCT 3 cut(s) 47, 106, 443
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 2 cut(s) 482, 715
AlwI GGATC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 614
ApeKI GCWGC 1 cut(s) 744
ApoI RAATTY 2 cut(s) 169, 682
AseI ATTAAT 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 793
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 88
BanI GGYRCC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 731
BccI CCATC 3 cut(s) 134, 318, 617
BcoDI GTCTC 2 cut(s) 482, 715
BcuI ACTAGT 2 cut(s) 133, 385
BfaI CTAG 4 cut(s) 44, 134, 386, 453
BfmI CTRYAG 2 cut(s) 13, 283
BglII AGATCT 1 cut(s) 66
BisI GCNGC 1 cut(s) 745
BlsI GCNGC 1 cut(s) 746
Bme18I GGWCC 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 1 cut(s) 793
BmiI GGNNCC 1 cut(s) 85
BmsI GCATC 2 cut(s) 136, 459
BmtI GCTAGC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 72, 463, 498, 613
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 615
Bse1I ACTGG 2 cut(s) 246, 332
Bse3DI GCAATG 1 cut(s) 18
BseDI CCNNGG 4 cut(s) 72, 463, 498, 613
BseGI GGATG 3 cut(s) 474, 609, 859
BseLI CCNNNNNNNGG 2 cut(s) 78, 615
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 246, 332
BseRI GAGGAG 2 cut(s) 602, 692
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 1 cut(s) 731
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 78, 615
BsmAI GTCTC 2 cut(s) 482, 715
BsmBI CGTCTC 2 cut(s) 482, 715
BsmFI GGGAC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 88, 462
Bsp143I GATC 3 cut(s) 66, 291, 448
BspACI CCGC 2 cut(s) 149, 259
BspHI TCATGA 1 cut(s) 214
BspLI GGNNCC 1 cut(s) 85
BspMAI CTGCAG 1 cut(s) 287
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 246, 332
BssECI CCNNGG 4 cut(s) 72, 463, 498, 613
BssMI GATC 3 cut(s) 66, 291, 448
BssT1I CCWWGG 2 cut(s) 72, 498
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 232, 435
BstF5I GGATG 3 cut(s) 474, 609, 859
BstKTI GATC 3 cut(s) 69, 294, 451
BstMAI GTCTC 2 cut(s) 482, 715
BstMBI GATC 3 cut(s) 66, 291, 448
BstMWI GCNNNNNNNGC 3 cut(s) 53, 83, 440
BstNSI RCATGY 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 13, 283
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 66, 448
BstYI RGATCY 2 cut(s) 66, 448
BtsCI GGATG 3 cut(s) 474, 609, 859
BtsIMutI CAGTG 2 cut(s) 339, 570
Cac8I GCNNGC 1 cut(s) 45
CciI TCATGA 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 793
CseI GACGC 1 cut(s) 697
Csp6I GTAC 1 cut(s) 305
CviAII CATG 5 cut(s) 53, 78, 215, 670, 691
CviJI RGCY 5 cut(s) 43, 47, 106, 443, 509
CviKI_1 RGCY 5 cut(s) 43, 47, 106, 443, 509
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 2 cut(s) 232, 435
DpnI GATC 3 cut(s) 68, 293, 450
DpnII GATC 3 cut(s) 66, 291, 448
Eco130I CCWWGG 2 cut(s) 72, 498
Eco32I GATATC 2 cut(s) 577, 727
Eco47I GGWCC 1 cut(s) 793
Eco57I CTGAAG 2 cut(s) 465, 495
Eco88I CYCGRG 1 cut(s) 614
EcoRI GAATTC 1 cut(s) 169
EcoRV GATATC 2 cut(s) 577, 727
EcoT14I CCWWGG 2 cut(s) 72, 498
ErhI CCWWGG 2 cut(s) 72, 498
Esp3I CGTCTC 2 cut(s) 482, 715
FaeI CATG 5 cut(s) 56, 81, 218, 673, 694
FaqI GGGAC 1 cut(s) 369
FatI CATG 5 cut(s) 52, 77, 214, 669, 690
FblI GTMKAC 2 cut(s) 12, 276
Fnu4HI GCNGC 1 cut(s) 745
FokI GGATG 3 cut(s) 481, 596, 866
Fsp4HI GCNGC 1 cut(s) 745
FspBI CTAG 4 cut(s) 44, 134, 386, 453
GluI GCNGC 1 cut(s) 745
GsuI CTGGAG 1 cut(s) 263
HgaI GACGC 1 cut(s) 697
Hin1II CATG 5 cut(s) 56, 81, 218, 673, 694
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 328, 494
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 13, 277
Hpy188I TCNGA 2 cut(s) 464, 514
Hpy188III TCNNGA 2 cut(s) 215, 321
Hpy8I GTNNAC 2 cut(s) 13, 277
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 23, 285, 472, 747, 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 83, 440
HpyF3I CTNAG 2 cut(s) 232, 435
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 5 cut(s) 56, 81, 218, 673, 694
Kzo9I GATC 3 cut(s) 66, 291, 448
LmnI GCTCC 2 cut(s) 465, 871
LpnPI CCDG 4 cut(s) 227, 271, 345, 847
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 2 cut(s) 136, 459
MaeI CTAG 4 cut(s) 44, 134, 386, 453
MaeII ACGT 1 cut(s) 30
MalI GATC 3 cut(s) 68, 293, 450
MboI GATC 3 cut(s) 66, 291, 448
MboII GAAGA 6 cut(s) 155, 166, 301, 458, 819, 846
MflI RGATCY 2 cut(s) 66, 448
MhlI GDGCHC 2 cut(s) 88, 462
MluCI AATT 9 cut(s) 169, 311, 343, 393, 519, 650, 682, 738, 762
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 831
MseI TTAA 3 cut(s) 342, 563, 737
MslI CAYNNNNRTG 1 cut(s) 695
MwoI GCNNNNNNNGC 3 cut(s) 53, 83, 440
NdeII GATC 3 cut(s) 66, 291, 448
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 5 cut(s) 56, 81, 218, 673, 694
NlaIV GGNNCC 1 cut(s) 85
NspI RCATGY 1 cut(s) 56
PagI TCATGA 1 cut(s) 214
PfeI GAWTC 1 cut(s) 328
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 746
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PshBI ATTAAT 1 cut(s) 737
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 793
PstI CTGCAG 1 cut(s) 287
PsuI RGATCY 2 cut(s) 66, 448
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 695
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 3 cut(s) 342, 563, 737
SatI GCNGC 1 cut(s) 745
Sau3AI GATC 3 cut(s) 66, 291, 448
Sau96I GGNCC 1 cut(s) 793
SchI GAGTC 1 cut(s) 488
SduI GDGCHC 2 cut(s) 88, 462
SetI ASST 9 cut(s) 33, 49, 108, 119, 276, 306, 445, 798, 832
SfaNI GCATC 2 cut(s) 136, 459
SfcI CTRYAG 2 cut(s) 13, 283
SinI GGWCC 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 695
SpeI ACTAGT 2 cut(s) 133, 385
Sse9I AATT 9 cut(s) 169, 311, 343, 393, 519, 650, 682, 738, 762
SsiI CCGC 2 cut(s) 149, 259
SspMI CTAG 4 cut(s) 44, 134, 386, 453
StyI CCWWGG 2 cut(s) 72, 498
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 276, 687
TasI AATT 9 cut(s) 169, 311, 343, 393, 519, 650, 682, 738, 762
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 3 cut(s) 342, 563, 737
Tru9I TTAA 3 cut(s) 342, 563, 737
TscAI CASTG 2 cut(s) 339, 577
TseI GCWGC 1 cut(s) 744
TspDTI ATGAA 3 cut(s) 417, 707, 815
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 2 cut(s) 339, 577
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 793
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 2 cut(s) 169, 682
XceI RCATGY 1 cut(s) 56
XmiI GTMKAC 2 cut(s) 12, 276
XspI CTAG 4 cut(s) 44, 134, 386, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.